CN105147746A - Application of glasswort extract - Google Patents

Abstract

The invention provides antioxidant properties of a glasswort extract, and studies the effect of the glasswort extract to generation of rat liver tissue homogenate and liver mitochondria MDA as well as rat red blood cell hemolysis. The main study results are shown as follows: when the dose of the glasswort extract is within the range of 0.5-10 mg.mL<-1>, the generation of the rat liver tissue homogenate and the liver mitochondria MDA, as well as the rat red blood cell hemolysis can be inhibited, and a dose-effect relationship is represented, so that the glasswort extract has an in vitro antioxidant effect; along with the dose addition of the glasswort extract, the activity of GSH-Px and SOD in S180 tumor-bearing mice serums is improved, and the MDA content is reduced, so that in vivo antioxidant activity of the tumor-bearing mice serums is improved. The effect on the promotion of the improvement of the tumor-bearing mice in-vivo mice antioxidant property generated by 300 mg.kg<-1>.d<-1> of the glasswort extract in dose is basically the same as that generated by 10 mL.kg<-1>.d<-1> of 5-FU.

Description

A kind of purposes of Salicornia Bigelovii Torr. extract

Technical field

The present invention relates to the technical field that Salicornia Bigelovii Torr. develops, be specifically related to Salicornia Bigelovii Torr. extract and develop for antioxidative.

Background technology

Salicornia Bigelovii Torr., the nearly 40-50 many kinds in the whole world is annual Chenopodiaceae herbaceous plant.Salicornia Bigelovii Torr. belongs to carnification euhalophyte, is generally grown on saline land, hag, salt lake take up seabeach.Salicornia Bigelovii Torr. is different from general sea water vegetable, and it is the plant that can become color in growth course, and after coming to the ripening period, Salicornia Bigelovii Torr. becomes red appearance from green clothing.At present, Salicornia Bigelovii Torr. is existing plantation in China's Coastal Areas, as: Yancheng etc., and start to carry out in flakes expanding the work of cultivating.Salicornia Bigelovii Torr. is biological detection is alkaline plant, and utilize natural sea-water to irrigate plantation, need not apply chemical reagent, be a kind of natural sea-water type vegetable of green.Salicornia Bigelovii Torr. also has multiple biological activity, its nutritional labeling is several times of similar vegetable, active substance especially higher than other land plants, except as except edible vegetable, also there is the effect of medical treatment, health care, have good value to various diseases such as treatment obesity, diabetes.

Salicornia Bigelovii Torr. is as vegetable, and be a kind of seasonal vegetable of delicious food, amino acid classes and content are all high, and is rich in edible fiber and vitamin and trace element, is nutritious.Its tender point is rich in vitamin C and mineral element, as trace element such as sodium, as iodine, manganese etc.At home and abroad often eaten by as vegetable, doubly popular to people.

Salicornia Bigelovii Torr. is as oil crop, and found by test, its seed yield can reach 1800 to 2250kg/km ², seed is more much higher than the oil yield of the oil crop seeds oil expressions such as Semen sojae atricolor containing fat content, and in oil, unsaturated fatty acid is very abundant, can be used as branded oil and eats.

Salicornia Bigelovii Torr. is used for feedstuff industry, and due to the edible irrigation carrying out sea water, plantation is got up more for convenience, and output is considerable.The straw of Salicornia Bigelovii Torr. in greenfeed, can replace wheat straw, is used for feeding domestic animal, can reach the object that benign cycle utilizes, and produces biogas, regenerative feedstuff, recycle the plantation in Salicornia Bigelovii Torr. as organic fertilizer by the feces processing domestic animal.Therefore, plant as feedstuff with Salicornia Bigelovii Torr., on the one hand can using water wisely, the problem of animal feeding-stuff can also be solved simultaneously.

The self-recovery of extensive plantation to ecological environment of Salicornia Bigelovii Torr. has certain effect of supervising.First, greenhouse gases CO can be absorbed from air ₂, its assimilation effect than Semen Tritici aestivi etc. better, therefore, reduces greenhouse effect Be very effective.Secondly, Salicornia Bigelovii Torr. plant has the effect absorbing and purify soil, has certain purification to agricultural effluent, metallic wastewater.In addition, Salicornia Bigelovii Torr. development company also produces the product of Salicornia Bigelovii Torr. series, such as, the cosmetics of Salicornia Bigelovii Torr. moisturizing series, its effect is better; Crab grass-stalk is processed, makes wood-fiber products, for plank, or as fuel; After Salicornia Bigelovii Torr. is extracted oil soybean cake residue etc., by being processed into pulverizing, wherein protein content is more much higher than wheat flour, and wheat flour is wherein many containing the comparision contents of methyllanthionine, and essential amino acid also containing certain amount, is adapted at food service industry application etc.

At present, China is relatively less to the composition Study of Salicornia Bigelovii Torr., and research direction mainly concentrates on the aspects such as breeding to Salicornia Bigelovii Torr., cultivation and Mechanism of Salt-tolerant, relatively weak to this research on the one hand of physiologically active.

Summary of the invention

An object of the present invention is to provide the non-oxidizability of Salicornia Bigelovii Torr. extract.Mainly provide Salicornia Bigelovii Torr. extract to generate and the impact of Rat Erythrocytes haemolysis, for the development and utilization of Salicornia Bigelovii Torr. provides technical support liver tissues of rats homogenate, hepatic mitochondria MDA.

To achieve these goals, the technical scheme that the present invention proposes is:

1. Salicornia Bigelovii Torr. extract is at 0.5 ~ 10mg.mL ^-1in scope, the generation of liver tissues of rats homogenate and hepatic mitochondria MDA can be suppressed, suppress Rat Erythrocytes haemolysis, and in dose-effect relationship, show that Salicornia Bigelovii Torr. extract has Antioxidtive Activities in Vitro.

2. along with the increase of Salicornia Bigelovii Torr. extract doses, S ₁₈₀in tumor-bearing mice serum, GSH-Px and SOD activity improves, and MDA content reduces, thus improves antioxidant activity in its body; When dosage reaches 300mgkg ^-1d ^-1time, Salicornia Bigelovii Torr. extract and 10mLkg ^-1d ^-15-FU to the raising there was no significant difference promoting oxidation resistance in tumor-bearing mice body.

Compared with prior art, advantage of the present invention and beneficial effect are:

Current research and development utilizes the halophytes be grown on length and breadth of land coastal desert and seabeach, and become the key subjects of world today's agricultural, on salt-soda soil, farming is a kind of new mode of thinking.Because Salicornia Bigelovii Torr. growth is on the salt-soda soil of barren land, cost of land is lower.In addition, it is without insect pest, and do not need with pesticide, do not need to hoe up weeds, Salicornia Bigelovii Torr. Cultivate administration expense is not high yet.Therefore, Salicornia Bigelovii Torr. extract wide material sources, and be comparatively safe, search out a kind of efficient and safe antioxidant and come preservation fruit and vegerable and seafood product, there is very important practical application meaning in food processing industry.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is further described, but this example just lifted for the ease of understanding, should not be considered to be limitation of the scope of the invention.Equally; any person of ordinary skill in the field all can according to the description of technical scheme of the present invention and preferred embodiment thereof; make various possible equivalent change or replacement, but all these change or replace the protection domain that all should belong to claim of the present invention.

The antioxidation in vitro research of embodiment 1 Salicornia Bigelovii Torr. extract

1. Salicornia Bigelovii Torr. extract is on the impact of liver tissues of rats homogenate external generation MDA

Compared with the control, Salicornia Bigelovii Torr. extract doses is at 0.5 ~ 10mg.mL ^-1in scope, to the generation of MDA in rat liver homogenate, there is significant inhibitory action, and present dose-effect relationship; When dosage reaches 5mg.mL ^-1time, suppression ratio reaches 28.35%.

2. Salicornia Bigelovii Torr. extract is on the impact of rat liver mitochondria lipid peroxidation

Compared with the control, after the effect of Salicornia Bigelovii Torr. extract, the generation of rat liver mitochondria MDA can be made significantly to decline, effectively inhibit the generation of MDA, and in dose-effect relationship; When dosage reaches 5mg.mL ^-1time, suppression ratio reaches 29.62%, and comparatively dosage is 0.5mg.mL ^-1time suppression ratio significantly improve.

3 Salicornia Bigelovii Torr. extracts are on the impact of erythrocyte (RBC) from haemolysis

When dosage reaches 2.5mg.mL ^-1time, significant difference between Salicornia Bigelovii Torr. extract each dosage group light absorption value and matched group, shows that it is all inhibited to the outer autoxidation hemolytic reaction of red cell body, and presents dose-dependence, but along with the further increase of dosage, suppression ratio improves not remarkable; When dosage reaches 10mg.mL ^-1time, the haemolysis suppression ratio of Salicornia Bigelovii Torr. extract to mouse red blood cell reaches 42.93%.

Embodiment 2 Salicornia Bigelovii Torr. extract is to S ₁₈₀the impact of antioxidant activity in tumor-bearing mice body

Table 1 Salicornia Bigelovii Torr. extract is to S ₁₈₀the impact of SOD, GSH-Px and MDA in mice serum

As shown in Table 1, the MDA of Salicornia Bigelovii Torr. extract group generates and all declines gradually along with the increase of dosage.Salicornia Bigelovii Torr. extract group and Normal group, model control group all have significant difference in MDA generation; Salicornia Bigelovii Torr. extract doses reaches 300mgkg ^-1d ^-1time, MDA growing amount significantly lower than positive control 5-FU, is 3.55nmolmL ^-1.

Table 1 shows, along with the increase of Salicornia Bigelovii Torr. extract doses, in the mice serum of each dosage group, SOD activity rises thereupon, reaches 300mgkg at dosage ^-1d ^-1time, with the SOD activity of model control group, there is significant difference, reach 200mgkg ^-1d ^-1time, SOD active with positive control 5-FU without significant difference.

As shown in table 1, show the activity research of GSH-Px in mice serum, each dosage group of Salicornia Bigelovii Torr. extract is significant difference between product GSH-Px activity, and in dose-effect relationship, has the significance difference opposite sex compared with Normal group; GSH-Px activity all has significant difference with model control group, shows that the GSH-Px that Salicornia Bigelovii Torr. extract can improve in S180 tumor-bearing mice body is active; Salicornia Bigelovii Torr. extract reaches 300mgkg at dosage ^-1d ^-1time, produce GSH-Px activity and reach 506.05UmL ^-1, and positive control 5-FU group is 507.88UmL ^-1, difference is not remarkable, shows that Salicornia Bigelovii Torr. extract has antioxidant effect in good body.

Claims (2)

1. Salicornia Bigelovii Torr. extract is at 0.5 ~ 10mg.mL ^-1in scope, the generation of liver tissues of rats homogenate and hepatic mitochondria MDA can be suppressed, suppress Rat Erythrocytes haemolysis, and in dose-effect relationship, show that Salicornia Bigelovii Torr. extract has Antioxidtive Activities in Vitro.

2. along with the increase of Salicornia Bigelovii Torr. extract doses, S ₁₈₀in tumor-bearing mice serum, GSH-Px and SOD activity improves, and MDA content reduces, thus improves antioxidant activity in its body; When dosage reaches 300mgkg ^-1d ^-1time, Salicornia Bigelovii Torr. extract and 10mLkg ^-1d ^-15-FU to the raising there was no significant difference promoting oxidation resistance in tumor-bearing mice body.