CN105145360A - Rapid propagation method for tomato test tube seedling capable of improving proliferation rate - Google Patents
Rapid propagation method for tomato test tube seedling capable of improving proliferation rate Download PDFInfo
- Publication number
- CN105145360A CN105145360A CN201510590003.XA CN201510590003A CN105145360A CN 105145360 A CN105145360 A CN 105145360A CN 201510590003 A CN201510590003 A CN 201510590003A CN 105145360 A CN105145360 A CN 105145360A
- Authority
- CN
- China
- Prior art keywords
- tomato
- concentration
- test
- tube plantlet
- callus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a rapid propagation method for a tomato test tube seedling capable of improving proliferation rate. The rapid propagation method comprises an explant processing step and a subculture multiplication step. According to the rapid propagation method, a lot of tomato test tube seedlings can be rapidly obtained; and the obtained tomato test tube seedlings have the advantages of strong growth force and high quality. Therefore, a good foundation is laid for tomato biotechnology research, industrial and large-scale rapid tomato seedling propagation and the like.
Description
Technical field
The present invention is specifically related to a kind of tomato test-tube plantlet fast breeding method improving the rate of increase.
Background technology
Tomato is the annual herb plant that Solanaceae tomato belongs to tomato species, is main culturing vegetable.The features such as it has numerous in variety, and wide accommodation, output are high, nutritious, vegetative period is long, therefore its area under cultivation constantly expands.Along with the continuous increase of area under cultivation, the kind of tomato is constantly updating, and new varieties are constantly applied in production.At present, tomato cultivation is main mainly with seedling, is easily subject to the harm of arid, cold and multiple damage by disease and insect etc., causes the resistance of breeding reduce and affect yield and quality; Simultaneously tomato seeds is almost F1 generation hybrid, needs the annual production of hybrid seeds or from external import seed, seed costs can be in any more, increases production cost.Utilize tissue culture technique to carry out the problem such as seedling breeding, transgeneic procedure, crossbreeding, Germ-plasma resources protection of Fast-propagation to tomato and all have larger meaning, production cost can be reduced, keep its merit, there is provided a large amount of seedling to market in a short time, there is very high economic worth.But in tomato tissue cultivation is numerous soon, during its test-tube plantlet propagation, apical dominance is very strong, little generation Multiple Buds or from joint sprout, usually carry out expansion with stem section numerous, so that reproduction coefficient is lower, object numerous soon in production can not be reached, for this reason, on the basis of cultivating early stage, its culture technique and condition are optimized, to increase effective joint number of tomato test-tube plantlet, filter out suitable culture medium prescription, thus improve its growth coefficient, to meet the needs of production.
Summary of the invention
The technical problem to be solved in the present invention, is to provide a kind of tomato test-tube plantlet fast breeding method improving the rate of increase.
The present invention is achieved in that a kind of tomato test-tube plantlet fast breeding method improving the rate of increase, comprises the following steps:
(1) explant process: get cultivation 25 ~ 30d and the tomato Shoots in vitro of robust growth, excision blade after the petiole staying 0.5cm long, 45° angle is cut sth. askew the explant that the stem-segment with node of getting 0.5 ~ 1.0cm is bred as tomato Plantlet subculture;
(2) shoot proliferation is cultivated: be inoculated in subculture multiplication medium by explant 45° angle oblique cutting, and in temperature 25 ± 1 DEG C, intensity of illumination is the LED of 3000 ~ 5000lx, cultivates 28d under the condition of illumination every day 12h, obtains tomato Shoots in vitro new after breeding.
Further, described subculture multiplication medium comprises following compositions: MS medium, 6-benzyl aminoadenine 0.01mg/L, indolebutyric acid 0.5mg/L, sucrose 30g/L and agar powder 6g/L; Wherein, the concentration of each composition all with the volume of the total solution of subculture multiplication medium for benchmark.
The invention has the advantages that: a large amount of tomato test-tube plantlet can not only be obtained fast; and the tomato test-tube plantlet obtained has the strong and high-quality advantage of growing power, thus have laid a good foundation for tomato biological technical research and industrial massive tomato seedling fast breeding etc.
Embodiment
One, improve a tomato test-tube plantlet fast breeding method for the rate of increase, comprise the following steps:
(1) explant process: under aseptic condition, get cultivation 25 ~ 30d and the tomato Shoots in vitro of robust growth on aseptic filter paper, excision blade after the petiole staying 0.5cm long, 45° angle is cut sth. askew the explant that the stem-segment with node of getting 0.5 ~ 1.0cm is bred as tomato Plantlet subculture;
(2) shoot proliferation cultivate: by explant 45° angle oblique cutting and axillalry bud be inoculated in upward in subculture multiplication medium, and in temperature 25 ± 1 DEG C, intensity of illumination is the LED of 3000 ~ 5000lx, cultivates 28d under the condition of illumination every day 12h, obtains tomato Shoots in vitro new after breeding.
Described subculture multiplication medium comprises following compositions: MS medium, 6-benzyl aminoadenine 0.01 (BA) mg/L, indolebutyric acid 0.5mg/L, sucrose 30g/L and agar powder 6g/L; Wherein, the concentration of each composition all with the volume of the total solution of subculture multiplication medium for benchmark.
Described MS medium specifically comprises following composition: ammonium nitrate (NH
4nO
3) 1650mg/L, potassium nitrate (KNO
3) 1900mg/L, calcium chloride (CaCl
22H
2o) 440mg/L, magnesium sulfate (MgSO
47H
2o) 370mg/L, potassium dihydrogen phosphate (KH
2pO
4) 170mg/L, manganese sulphate (MnSO
4h
2o) 22.3mg/L, zinc sulphate (ZnSO
47H
2o) 8.6mg/L, cobalt chloride (CoCl
26H
2o) 0.025mg/L, copper sulphate (CuSO
45H
2o) 0.02mg/L, boric acid (H
3bO
3) 6.2, sodium molybdate (Na
2moO
42H
2o) 0.25mg/L, potassium iodide (KI) 0.83mg/L, ferrous sulfate (FeSO
47H
2o) 28.7mg/L, disodium ethylene diamine tetraacetate (Na
2-EDTA) 37.3mg/L, nicotinic acid 0.5mg/L, Cobastab
60.5mg/L, Cobastab
10.1mg/L, inositol 100mg/L, glycine 2mg/L.
Two, tomato test-tube plantlet inoculation technique is optimized
In tomato Plantlet subculture proliferation test process, find blade-carrying explant, often the axillalry bud of inoculation stem section can cannot be sprouted because of the nutrient competition of blade, greatly can reduce sprouting; If all removed by petiole, the bud point meeting blackening of most axillalry buds, also can be reduced germination rate greatly, be shown by test, and when the petiole of stem-segment with node retains about 0.5cm, in suitable medium, most axillalry buds can normally be sprouted.
In tomato Plantlet subculture proliferation test process, find crosscut and cut sth. askew larger on the impact of test-tube plantlet late growing stage.During crosscut, the test-tube plantlet later stage can show comparatively thin and weak, and 45° angle is cut sth. askew, and later stage test-tube plantlet growth can be strongr, and this may have relation with the contact surface of explant and medium, and contact surface is large, easily absorbs more nutrient.Research also finds, inoculation adopts 45° angle oblique cutting and axillalry bud mode upward, and the growing state of its test-tube plantlet is obviously better than the effect of vertical inoculation, and this may be that oblique cutting inoculation fully can ensure axillalry bud top all the time upward, and the contact surface of oblique cutting and medium is large, increase the absorption of nutrient.
Three, indolebutyric acid (IBA) concentration optimization in medium in tomato Multiplying culture process
1, the medium of additional different I BA concentration
The working concentration of this test to IBA is optimized, and in the subculture multiplication medium that tomato test-tube plantlet stem section is inoculated into additional different I BA concentration and J1 ~ J6 medium, observes its bud seedling growing state, record the data of each observation item after cultivating 28d.Culture medium prescription is as follows:
J1：MS+BA0.1mg/L+IBA0.01mg/L
J2：MS+BA0.1mg/L+IBA0.05mg/L
J3：MS+BA0.1mg/L+IBA0.1mg/L
J4：MS+BA0.1mg/L+IBA0.2mg/L
J5：MS+BA0.1mg/L+IBA0.5mg/L
J6：MS+BA0.1mg/L+IBA1.0mg/L
Above medium all adds sucrose 30g/L, agar powder 6g/L, and pH is adjusted to about 5.8.
2, different I BA concentration impact that tomato stem segment with axillary buds is sprouted
Above-mentioned tomato explant is inoculated in above-mentioned subculture multiplication medium J1 ~ J6, after cultivating 28d, from tomato test-tube plantlet stem segment with axillary buds germination rate (see table 1), when IBA concentration is 0.01 ~ 0.5mg/L and subculture multiplication medium J1 ~ J5, along with the increase of IBA concentration, the variation tendency of the germination rate of tomato stem segment with axillary buds is first raise to reduce afterwards to raise rear reduction again, two germination rate height points are respectively 98.89% and 82.22%, corresponding IBA concentration is respectively 0.05mg/L and 0.5mg/L (i.e. subculture multiplication medium J2 and J5), certain difference is there is between them, when IBA concentration is elevated to 1.0mg/L (i.e. subculture multiplication medium J6), decline extremely significantly when tomato stem segment with axillary buds germination rate and IBA concentration 0.5mg/L.This illustrates that IBA concentration sprouting on tomato axillalry bud has larger impact, wherein with IBA concentration for 0.05mg/L and 0.5mg/L time, germination rate is higher.
Table 1 tomato test-tube plantlet stem segment with axillary buds sprouts situation
Note: the different letter representation Deng Kenshi multiple range test significance of difference in table, wherein capitalization is difference extremely remarkable (P<0.01), and lowercase is significant difference (P<0.05).Lower same.
3, different I BA concentration is on the impact of tomato test-tube plantlet plant height, effectively joint number and the number of blade
Tomato explant mentioned above is inoculated in above-mentioned subculture multiplication medium J1 ~ J6 medium, after cultivating 28d, from tomato test-tube plantlet plant plant height, effectively joint number and the number of blade (see table 2).As can be seen from Table 2, IBA concentration at 0.01 ~ 1.0mg/L time, the plant height of tomato test-tube plantlet raises the trend of rapid reduction afterwards again in first reducing, average plant height is up to 6.21cm, and now IBA concentration is 0.5mg/L.
Because tomato test-tube plantlet apical dominance is obvious, the incidence of clump bud is low, and propagation mainly realizes by sections, and therefore this test has carried out statistical analysis to effective joint number of tomato test-tube plantlet and the number of blade.From the effective joint number of tomato test-tube plantlet plant, when IBA concentration is 0.01 ~ 1.0mg/L, the trend that the effective joint number of tomato test-tube plantlet reduces in increase after first minimizing again, when joint number is maximum, the working concentration of IBA is 0.5mg/L, and mean number is 3.79.
As can be seen from Table 2, with the rising of IBA concentration, the change of tomato test-tube plantlet blade number is consistent with the change of joint number, and in the trend that increase after first minimizing reduces again, average leaf number mostly is 5.92 most, and corresponding IBA concentration is also 0.5mg/L.
The situation of change of table 2 tomato test-tube plantlet plant height, effectively joint number and the number of blade
4, different I BA concentration is on the impact of tomato them Callus formation
In tomato test-tube plantlet incubation; its base portion often can form callus; base portion callus can affect the normal growth of tomato test-tube plantlet with test-tube plantlet competition for nutrients; after them grows callus simultaneously; its root also major part grows from callus, can affect the survival rate of test-tube plantlet.The working concentration of the generation of callus and size and growth hormone is closely related, therefore, is carrying out in IBA concentration tests, is observing the situation of tomato them callus, to determine the best working concentration of IBA.During at record Tomato Calli, a situation arises, have employed two indices, namely them callus incidence and callus index are (according to the size of callus, a little callus is designated as "+", comparatively small callus is designated as " ++ ", moderate size is designated as " +++ ", agglomerate is designated as " ++++", by that analogy).A situation arises in table 3 for tomato them callus, as can be seen from Table 3, the change of tomato them callus incidence shows as first to raise to reduce afterwards and raises rear " M " shape trend reduced again, the highest IBA of appearing at concentration be 0.05mg/L (J2 medium) and 0.1mg/L (J3 medium) time, incidence is 90.00%, IBA takes second place in 0.5mg/L (J5 medium) time, callus incidence is 83.33%, in J1 medium (IBA is 0.01mg/L), callus incidence is minimum 53.33%, this illustrates that the growth hormone of high concentration can induce tomato them to produce more callus.As can be seen from callus index, although the incidence of J2 and J3 callus is high, them callus is less, does not have too large impact to test-tube plantlet growth.
Comprehensive above tomato test-tube plantlet indices is analyzed, and in conjunction with the color of test-tube plantlet stem thickness, blade, finally determines that the IBA concentration of applicable tomato test-tube plantlet fast breeding is 0.5mg/L.
A situation arises for table 3 tomato them callus
Four, the optimization of the basic element of cell division (CTK) kind and concentration in tomato test-tube plantlet propagation
1, the medium of additional different CTK kind and concentration
In tomato test-tube plantlet breeding, its growth is subject to the tremendous influence of the basic element of cell division (CTK), in order to improve the rate of increase, studying, optimize the medium of tomato test-tube plantlet propagation to the kind of the basic element of cell division and concentration.Culture medium prescription is as follows:
BA1：MS+BA0.01mg/L+IBA0.5mg/L
BA2：MS+BA0.05mg/L+IBA0.5mg/L
BA3：MS+BA0.1mg/L+IBA0.5mg/L
BA4：MS+BA0.5mg/L+IBA0.5mg/L
BA5：MS+BA1.0mg/L+IBA0.5mg/L
KT1：MS+KT0.1mg/L+IBA0.5mg/L
KT2：MS+KT0.5mg/L+IBA0.5mg/L
KT3：MS+KT1.0mg/L+IBA0.5mg/L
TDZ1：MS+TDZ0.1mg/L+IBA0.5mg/L
TDZ2：MS+TDZ0.5mg/L+IBA0.5mg/L
TDZ3：MS+TDZ1.0mg/L+IBA0.5mg/L
ZT1：MS+ZT0.1mg/L+IBA0.5mg/L
ZT2：MS+ZT0.5mg/L+IBA0.5mg/L
ZT3：MS+ZT1.0mg/L+IBA0.5mg/L
The above KT is KT, TDZ is Thidiazuron, ZT is zeatin.The above medium all adds sucrose 30g/L, agar powder 6g/L, and pH is adjusted to about 5.8.
2, different CTK kind and concentration impact that tomato stem segment with axillary buds is sprouted
Tomato explant mentioned above is inoculated in 14 kinds of above-mentioned subculture multiplication medium, after cultivating 28d, from tomato test-tube plantlet stem segment with axillary buds germination rate (see table 4), BA concentration is when 0.01 ~ 1.0mg/L, the trend that tomato test-tube plantlet stem segment with axillary buds germination rate reduces in rising after first reduction again, tomato test-tube plantlet stem segment with axillary buds germination rate best result is not 91.11% and 82.22%, and corresponding BA concentration is respectively 0.1mg/L and 0.05mg/L; When KT concentration is 0.1mg/L ~ 1.0mg/L, tomato test-tube plantlet stem section germination rate is all the trend raised with the increase of KT concentration; When TDZ concentration is 0.1mg/L ~ 1.0mg/L, tomato test-tube plantlet stem section germination rate increases in downward trend with concentration, but the amplitude of change is smaller; When ZT concentration is 0.1mg/L ~ 1.0mg/L, tomato test-tube plantlet stem section germination rate is in downward trend slightly after first rising.Analysis result shows, from the germination rate of tomato test-tube plantlet stem section, the BA (0.01mg/L) of low concentration, the TDZ (0.1mg/L ~ 0.5mg/L) of low concentration, ZT (0.1mg/L ~ 1.0mg/L) are comparatively suitable as the basic element of cell division that induction tomato test-tube plantlet stem segment with axillary buds is sprouted, but the concentration range that they are suitable for has notable difference; The ZT (0.1mg/L) of BA (0.1mg/L ~ 1.0mg/L), high concentration KT (1.0mg/L), TDZ (0.1mg/L ~ 1.0mg/L), low concentration is comparatively suitable as the basic element of cell division of induction Man Xina test-tube plantlet stem segment with axillary buds sprouting.This illustrates that the basic element of cell division of variety classes and concentration is sprouted tomato test-tube plantlet stem segment with axillary buds and all has a great impact, and single from germination rate, what it had applicable tomato test-tube plantlet stem section to sprout under different concentration may.
Table 4 tomato test-tube plantlet stem segment with axillary buds sprouts situation
3, different CTK kind and concentration are on the impact of tomato test-tube plantlet plant height, effectively joint number and the number of blade
From tomato test-tube plantlet plant plant height, after cultivating 28d, tomato test-tube plantlet plant height situation is in table 5.As can be seen from Table 5, when BA concentration is 0.01mg/L ~ 1.0mg/L, the change that tomato test-tube plantlet plant height reduces in rising after first reduction again; When KT concentration is 0.1mg/L ~ 1.0mg/L, raises tomato test-tube plantlet plant height with concentration and show the rear rising trend of first reduction; When TDZ concentration is 0.1mg/L ~ 1.0mg/L, raise with concentration, tomato test-tube plantlet plant height is all downward trend, and this downward trend highly significant; When ZT concentration is 0.1mg/L ~ 1.0mg/L, tomato test-tube plantlet plant height raises with concentration and shows ever-increasing trend, and the trend of this increase is very remarkable.This illustrates that the basic element of cell division of variety classes and concentration has a significant impact tomato test-tube plantlet plant height.
From tomato test-tube plantlet plant joint number and the number of blade, after cultivating 28d, the effective joint number of tomato test tube and the number of blade are in table 5.As can be seen from Table 5, in the medium adding variety classes and cytokinin, the effective joint number of tomato test-tube plantlet and the number of blade have identical variation tendency.During BA concentration 0.01mg/L ~ 1.0mg/L, the trend that the effective joint number of tomato test-tube plantlet reduces in rising after first reduction again, the number of blade presents the change first reducing and raise afterwards; During KT concentration 0.1mg/L ~ 1.0mg/L, the effective joint number of tomato test-tube plantlet and the number of blade are in first reducing the change raised afterwards, but amplitude of variation is little; During TDZ concentration 0.1mg/L ~ 1.0mg/L, with the rising of concentration, the effective joint number of tomato test-tube plantlet and the number of blade are all downward trend, and as can be seen from tendency chart, low concentration TDZ (0.1mg/L) is conducive to test-tube plantlet growth.During ZT concentration 0.1mg/L ~ 1.0mg/L, raise with concentration, the effective joint number of test-tube plantlet and the number of blade are in the trend raised afterwards that first declines, and the ZT (0.1mg/L) of low concentration is conducive to the growth of test-tube plantlet.This illustrates that the impact of the basic element of cell division on the effective joint number of tomato test-tube plantlet and the number of blade of variety classes and concentration is also very large.
The situation of change of table 5 tomato test-tube plantlet plant height, effectively joint number and the number of blade
4, different CTK kind and concentration are on the impact of tomato them Callus formation
During at record Tomato Calli, a situation arises, also use two indices, namely them callus incidence and callus index are (according to the size of callus, a little callus is designated as "+", comparatively small callus is designated as " ++ ", moderate size is designated as " +++ ", agglomerate is designated as " ++++", by that analogy).A situation arises in table 6 for tomato them callus.As can be seen from Table 6, during BA concentration 0.01mg/L ~ 1.0mg/L, along with BA concentration increases, them callus incidence raises rapidly from very low level, to reaching during 0.5mg/L the highest (incidence 84.45%), decline rapidly again subsequently, but callus index is always in the trend risen, the amplitude just increased after 0.5mg/L is very little, this illustrates that the BA of low concentration effectively can control tomato them and form callus, although high concentration callus incidence declines, but base portion has the test-tube plantlet of long callus can produce huge callus, have a strong impact on the growth of test-tube plantlet.Concerning KT, when KT concentration is 0.1mg/L, tomato them does not produce callus, and with the rising of concentration, base portion callus incidence also increases sharply.TDZ has the effect strongly impelling tomato them callus to occur, and when its concentration 0.1mg/L ~ 1.0mg/L, them callus incidence is 88.89% ~ 95.56%.ZT has the effect strongly impelling its base portion to produce callus, and when concentration is 0.1mg/L ~ 1.0mg/L, its callus incidence is 73.33 ~ 97.78%.This illustrates that different basic element of cell division kinds and concentration have obvious difference to tomato them calli induction.
In sum, in conjunction with indexs such as test-tube plantlet growth gesture, leaf colors, the cell division being suitable as tomato test-tube plantlet propagation have BA and KT, wherein with BA concentration for 0.01mg/L is best.
A situation arises for table 6 tomato them callus
As can be seen from the above result to IBA concentration optimization and CTK kind and concentration optimization, the best culture medium prescription of tomato Plantlet subculture propagation is: MS+BA0.01mg/L+IBA0.5mg/L.
Claims (2)
1. can improve a tomato test-tube plantlet fast breeding method for the rate of increase, it is characterized in that: comprise the following steps:
(1) explant process: get cultivation 25 ~ 30d and the tomato Shoots in vitro of robust growth, excision blade after the petiole staying 0.5cm long, 45° angle is cut sth. askew the explant that the stem-segment with node of getting 0.5 ~ 1.0cm is bred as tomato Plantlet subculture;
(2) shoot proliferation is cultivated: be inoculated in subculture multiplication medium by explant 45° angle oblique cutting, and in temperature 25 ± 1 DEG C, intensity of illumination is the LED of 3000 ~ 5000lx, cultivates 28d under the condition of illumination every day 12h, obtains tomato Shoots in vitro new after breeding.
2. a kind of tomato test-tube plantlet fast breeding method improving the rate of increase as claimed in claim 1, is characterized in that: described subculture multiplication medium comprises following compositions: MS medium, 6-benzyl aminoadenine 0.01mg/L, indolebutyric acid 0.5mg/L, sucrose 30g/L and agar powder 6g/L; Wherein, the concentration of each composition all with the volume of the total solution of subculture multiplication medium for benchmark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510590003.XA CN105145360A (en) | 2015-09-17 | 2015-09-17 | Rapid propagation method for tomato test tube seedling capable of improving proliferation rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510590003.XA CN105145360A (en) | 2015-09-17 | 2015-09-17 | Rapid propagation method for tomato test tube seedling capable of improving proliferation rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105145360A true CN105145360A (en) | 2015-12-16 |
Family
ID=54786762
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510590003.XA Pending CN105145360A (en) | 2015-09-17 | 2015-09-17 | Rapid propagation method for tomato test tube seedling capable of improving proliferation rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105145360A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439378A (en) * | 2017-08-30 | 2017-12-08 | 福建省农业科学院农业工程技术研究所 | A kind of method of tomato test tube seedling high efficiently multiplying |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731144A (en) * | 2008-11-07 | 2010-06-16 | 中国科学院遗传与发育生物学研究所 | Method for culturing tomato tissues in test tube |
| CN104137779A (en) * | 2014-08-04 | 2014-11-12 | 江西省科学院生物资源研究所 | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly |
| CN104719136A (en) * | 2015-02-12 | 2015-06-24 | 上海杉一植物科技有限公司 | Rooting culture method of catalpa bungei tissue culture seedling |
| CN104782497A (en) * | 2015-05-02 | 2015-07-22 | 冯文杰 | In-vitro culture method for ornamental clematis L. |
-
2015
- 2015-09-17 CN CN201510590003.XA patent/CN105145360A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731144A (en) * | 2008-11-07 | 2010-06-16 | 中国科学院遗传与发育生物学研究所 | Method for culturing tomato tissues in test tube |
| CN104137779A (en) * | 2014-08-04 | 2014-11-12 | 江西省科学院生物资源研究所 | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly |
| CN104719136A (en) * | 2015-02-12 | 2015-06-24 | 上海杉一植物科技有限公司 | Rooting culture method of catalpa bungei tissue culture seedling |
| CN104782497A (en) * | 2015-05-02 | 2015-07-22 | 冯文杰 | In-vitro culture method for ornamental clematis L. |
Non-Patent Citations (1)
| Title |
|---|
| 李铁松等: "番茄外植体诱导直接分化不定芽建立高频再生系统", 《辽宁师范大学学报（自然科学版）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107439378A (en) * | 2017-08-30 | 2017-12-08 | 福建省农业科学院农业工程技术研究所 | A kind of method of tomato test tube seedling high efficiently multiplying |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102577969B (en) | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 | |
| CN101574055A (en) | Lonicera edulis tissue culturing method | |
| Moqbeli et al. | In vitro cucumber haploid line generation in several new cultivars | |
| CN107821165A (en) | A kind of tissue culture propagation and purposes for ceasing the tuber of pinellia | |
| Moshtaghi | Tissue and cell culture of saffron | |
| CN112042541B (en) | Method for propagating taxillus through somatic embryogenesis | |
| CN103548676A (en) | Tissue culture rapid propagation method for acer rubrum | |
| CN102577970B (en) | Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts | |
| CN101904302B (en) | Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill | |
| Singh et al. | Plant regeneration from alginate-encapsulated somatic embryos of Dalbergia sissoo Roxb. | |
| CN1233223C (en) | Screening method of salt enduring species of tomato | |
| CN105145360A (en) | Rapid propagation method for tomato test tube seedling capable of improving proliferation rate | |
| CN104094852B (en) | A kind of method of cassava plantlet in vitro strengthening seedling and rooting | |
| CN107439378A (en) | A kind of method of tomato test tube seedling high efficiently multiplying | |
| CN104620993A (en) | Method for rejuvenating degenerated gerbera jamesonii bolus | |
| CN112106660B (en) | Culture medium and culture method for improving potato propagation process efficiency | |
| Kumar et al. | Tissue culture studies in two important orchid taxa: Rhynchostylis retusa (L.) Bl. and Cymbidium elegans Lindl | |
| CN109169285B (en) | Method for culturing immature seeds of hot peppers and rapidly propagating seedlings | |
| CN102742503A (en) | Method for induction of test-tube seedling Dioscorea Opposita Thunb. beans and seedling formation | |
| CN103688861A (en) | Lilium oriental hybrid tissue culture seedling strengthening method | |
| CN102934613A (en) | Breeding method for inducing photinia serrulata somatic cell by using 60Co r ray radiation | |
| Lal et al. | Comparitive economics of in vitro grown plantlets of clonal apple MM 106 and MM 111 rootstocks | |
| CN104871978A (en) | Method for obtaining aseptic seedlings of ruby red flower aesculus | |
| CN105284621B (en) | A kind of method that a large amount of drepanostachyum luodianense regeneration plants are obtained by body embryo culture | |
| Zeng et al. | High efficiency in vitro plant regeneration from epicotyl explants of Ponkan Mandarin (C itrus reticulata Blanco) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151216 |