CN105137067B - Detection device - Google Patents
Detection device Download PDFInfo
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- CN105137067B CN105137067B CN201510275018.7A CN201510275018A CN105137067B CN 105137067 B CN105137067 B CN 105137067B CN 201510275018 A CN201510275018 A CN 201510275018A CN 105137067 B CN105137067 B CN 105137067B
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- analyte
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
Abstract
The invention provides a detection device. The detection device comprises a carrier which supports liquid flow; the carrier comprises an analyzed object binding area, a reaction reagent area and a sealing reagent area; the analyzed object binding area comprises a specific binding molecule; the sealing reagent area comprises one or more sealing reagents; the reaction reagent area comprises one or more reaction reagents for completing reactions; and the sealing reagent makes contact with the specific binding molecule of the analyzed object binding area prior to one reaction reagent; the reaction reagent area is located at the upstream of the analyzed object binding area, and the sealing reagent area is perpendicular to the arrangement direction of the reaction reagent area and the analyzed object binding area. The device can reduce detection false positive results and improve the detection accuracy.
Description
Technical field
It, with regard to detection means, is with regard to whether depositing analyte in a kind of analysis fluid sample more specifically that the present invention is
The detection means of matter.
Background technology
Increasingly popularized come the device of analyte in detection sample using horizontal mobility immunoreagent bar, but, detection
Accuracy still need and improve.Especially, when some antibacterials in detection sample or virus antigen or antibody when
Wait, need accurately acquisition testing result even more important.For example, blood sample streptococcus intermedius is detected using double antibody principle
In the immunoassay device of (Strep A) antigen, it usually needs a kind of antibody of conjugated antigen is fixed on carrier, such as nitre
On acid cellulose film, some closed reagents, such as bovine serum albumin (BSA) or casein are then processed on carrier again
(Casein), then it is finally prepared to dry carrier or reagent strip again carrier drying.Although so processing can improve detection
Accuracy, when a large amount of large-scale productions, so process process it is excessively loaded down with trivial details, in addition, it is such process some are divided
The detection of analysis thing can not significantly improve the accuracy of detection, or false-positive result, such as hepatitis C virus easily occur
Malicious (HCV) antigen.
The content of the invention
In order to solve problem above, the present invention provides a kind of detection means and detection method, can significantly improve detection knot
The accuracy of fruit, while simplifying production technology.
On the one hand, the present invention provides a kind of detection means, including：The carrier of liquid flowing is supported, is included on carrier：One
Individual analyte land, a reaction reagent area；One closed reagent area；Described analyte land includes a kind of special
The binding molecule of the opposite sex；Include one or more closed reagents in described closed reagent area；Wrap in described reaction reagent area
Include one or more reaction reagents for completing to react；Wherein, described closed reagent connects in advance than a kind of reaction reagent therein
Touch the specific binding molecule on the analyte land.In a preferred mode, do not apply fluid sample to
Before detection means, each area is all present in a dry state on carrier, and after being moistened by liquid, closed reagent and reaction reagent are by liquid
Move body to analyte land.
In some specific modes, can be with order to allow closed reagent to contact the mode of analyte binding molecule in advance
It is that the distance between specific binding molecule on allowing closed reagent and analyte land is less than reaction reagent and analyte
The distance between specific binding molecule on land.In more specifically one mode, closed reagent area is allowed to tie positioned at analyte
Close the upstream in area and positioned at the downstream in reaction reagent area.Alternatively, it is also possible to allow closed reagent area to be located at analyte land
Downstream, the upstream of the analyte land that reaction reagent area is located at.It is also possible that reaction reagent area ties positioned at analyte
The upstream in area is closed, the direction that closed reagent area arranges with reaction reagent area and analyte land is vertical.
In some preferred modes, reaction reagent includes the molecule of the specific bond analyte with colored particle；
The molecule of specific bond analyte of the closed reagent ratio with colored particle is contacted in advance on described analyte land
Specific binding molecule.It is gold grain or latex particle with coloured particulate labels more specifically in mode.More have
In the mode of body, the specific binding molecule on analyte land resists for the first of analyte (analyte is antigen)
Body, the molecule of the specific bond analyte with colored particle is the second antibody of analyte.
In some concrete schemes, the binding molecule of specific bond analyte is fixed on carrier.Other one
In a little modes, the closed reagent and reaction reagent on enclosed area can be moved with liquid." fixation " mentioned here refers to those
Reagent is processed on carrier and can not move with liquid, and optimum is that the reagent of 90-100% can not be moved, and also may be used
Can be that the reagent of 80-95% can not be moved with liquid on carrier；It is also likely to be 60-90%.It is mentioned here that " movement " is referred to
The reagent that those are processed on carrier can be moved by liquid, and optimum is that the reagent of 80-100% is moved by liquid on carrier
It is dynamic；It is also likely to be that the reagent of 60-95% is moved by liquid, it is also possible to which the reagent of 1-60% is moved by liquid.
In some specific modes, closed reagent can be with reference on the specific binding molecule on analyte land
Nonspecific binding site.
On the other hand, the method that the present invention also provides analyte in a kind of analysis fluid sample, including：There is provided a kind of
The carrier of liquid flowing is supported, is included on the carrier：One analyte land, includes a kind of special on described land
Binding molecule；One or more complete the reaction reagent for reacting；Tie with specificity on analyte land with one or more
Close the closed reagent that the site on molecule combines；Detectable and closed reagent are allowed as liquid is on analyte land
Specific binding molecule is moved；Closed reagent is allowed to contact the specific bond on described analyte land in advance than reaction reagent
Molecule.
In a specific mode, specific binding molecules include specific bond analyte on analyte land
A kind of antibody or antibody fragment, another kind of antibody or fragment of reaction reagent including specific bond analyte.
In above all of embodiment, analyte land may be located on nitrocellulose filter.In addition, envelope
It can be one or two in serum albumin or casein to close reagent.Reaction reagent can also include colored particle marker
Thing.Analyte can include hepatitis C virus, hepatitis B virus, hepatitis B surface antigen or HIV (human immunodeficiency virus).Closed reagent can be carried
Positive charge.In addition, in above all of embodiment, each area on carrier and carrier can be before being detected
Dry state, when being detected, sample moistens carrier and last each area of carrier, wherein reaction reagent and closing examination
Agent can be moved by liquid.
Beneficial effect：
Using the device of the present invention, the accuracy of testing result can be significantly improved, while simplify producing the device
Production technology, reduces cost.
Description of the drawings
Fig. 1 is the plan structure of reagent strip in the conventional apparatus of antigen of showing one's high ideals in double antibody sandwich method detection sample using three
Schematic diagram, Figure 1A is the overlooking the structure diagram for not applying the detection means before fluid sample；1B is to apply after fluid sample
The overlooking the structure diagram of detection means.In detection means, including a carrier 100, combine including analyte on 100
Area 105, includes a kind of binding molecule 101 of special analyte, the first antibody of such as analyte on 105；With one
Individual reaction reagent area 106, includes another specific bond point of the specific bond analyte with mark substance on 106
Son, the second antibody of analyte；Analyte land and reaction reagent area are disposed in same liquid flow direction
Upstream and downstream.
When being detected, fluid sample is applied on carrier 100, allows it from reaction reagent region to analyzed
Thing land is moved, and in the case of moistening, liquid reaches specific bond area 105 with the reagent in reagent areas, if sample
There is analyte in product, the accumulation mark substance on analyte calmodulin binding domain CaM, so as to pass through to calculate mark substance accumulation
Number judging the number of analyte.
Fig. 2 is a specific embodiment of the present invention, and Fig. 2A is the vertical view for not applying the detection means before fluid sample
Structural representation；Fig. 2 B are the overlooking the structure diagram for applying the detection means after fluid sample.In detection means, including one
Individual single carrier 100, includes analyte land 105 on 100, and a species specific binding molecule is included on 105
101, and a reaction reagent area 106, another for including the specific bond analyte with mark substance on 106 is special
Binding molecule；Include closed reagent 103 with a closed area 104,104；Analyte land and reaction reagent area are by cloth
The upstream and downstream in same liquid flow direction is put, and enclosed area 104 is configured to (Fig. 2A) vertical with analyte land,
Reaction reagent area 106 is allowed to be more than closed reagent area 104 and analyte land with the distance of analyte land 105 simultaneously
105 distance, so allows flowing time of the fluid in two intervals different, so as to the time for exhibiting up to land 105 has
Successively.When being detected, fluid sample is applied on carrier 100, allows it from reaction reagent region to analyte
Land is moved, and fluid sample is applied on carrier 104 in addition, allows liquid to move to analyte land along carrier 104
Dynamic, such closed reagent takes analyte calmodulin binding domain CaM to and first combines some sites on specific binding molecule 101 by liquid,
In the case of moistening, liquid arrives soon after specific bond area 105 with the reagent in reagent areas, if exist in sample divided
Analysis material, the accumulation mark substance on analyte calmodulin binding domain CaM, so as to by calculate mark substance accumulation number judging
The number of analyte.
Fig. 3 is a specific embodiment of the present invention, and Fig. 3 A are the vertical view for not applying the detection means before fluid sample
Structural representation；Fig. 3 B are the overlooking the structure diagram for applying the detection means after fluid sample.In detection means, including one
Individual carrier 100, includes analyte land 105, the first of material being analysed including a kind of specific bond on 105 on 100
Antibody 101, and a reaction reagent area 106, include the another another of the specific bond analyte with mark substance on 106
Individual antibody；Include closed reagent 103 with a closed area 104,104；Analyte land and reaction reagent area are arranged
In the upstream and downstream of same liquid flow direction, and closed area be arranged on reaction reagent area and analyte land it
Between.
When being detected, in the reaction reagent area 106 that fluid sample is applied on carrier 100, it is allowed from anti-
Answer reagent areas to move to analyte land 105, due to the difference of position, the speed ratio of the moisture movement in liquid compared with
Hurry up, enclosed area is reached in advance, such closed reagent reaches analyte land and first combines first antibody 101 by carrier in advance
On some sites, it is also possible in advance some big holes on closing carrier, reduce these macropores and reaction reagent are cut
Obtain；In the case of moistening, liquid arrives soon after specific bond area 105 with labeled second antibody, if in sample not
There is analyte, nonspecific combination would not occur on analyte calmodulin binding domain CaM, not accumulation mark substance, from
And by calculate mark substance accumulation number come judge analyte number.
Fig. 4 depicts another preferred embodiment of the present invention.Fig. 4 A are the stereochemical structure of reagent strip structure
Schematic diagram, Fig. 4 B are the overlooking the structure diagram of reagent strip structure.In detection means, the carrier material for constituting sample pad 18 is
Glass fibre；The material for constituting label pad is polymer PET 12, and processing in label pad has the anti-hepatitis C with latex particle
The first Monclone antibody of virus；The material for constituting sealing pad is polymer PET 14, and processing on the film has casein
(Casein)；The material for constituting analyzed pad 15 is nitrocellulose filter, and specific bond the third type liver is secured on the film
Another kind of Monclone antibody 11 of scorching virus；With the filter paper for constituting adsorptive pads 17, they join end to end, and allow the fluid sample can be from
Sample pad 18 is flowed on adsorptive pads 17.
Fig. 5 is a specific embodiment of the present invention, and Fig. 5 A are the vertical view for not applying the detection means before fluid sample
Structural representation；Fig. 5 B are the overlooking the structure diagram for applying the detection means after fluid sample.In detection means, including one
Individual carrier 100, includes analyte land 105 on 100, includes that a kind of specific bond carries mark substance on 105
Binding molecule 101, and a reaction reagent area 106, include the spy of the specific bond analyte with mark substance on 106
Different binding molecule；Include closed reagent 103 with a closed reagent area 104,104；Tie positioned at analyte in reaction reagent area 106
Close the upstream in area；Closed reagent area is located at the distance of the downstream of analyte land, closed reagent area and specific binding molecule
Less than reaction reagent area and the distance of specific binding molecule.
When being detected, fluid sample while in the reaction reagent area 106 being applied on carrier 100 and closing
Qu Shang, such closed reagent reaches analyte land first with reference to some on specific binding molecule 101 by carrier in advance
Site, in the case of moistening, liquid arrives soon after specific bond area 105 with the reagent in reagent areas, if in sample
There is analyte, the accumulation mark substance on analyte calmodulin binding domain CaM, so as to pass through to calculate many of mark substance accumulation
Less judging the number of analyte.
Fig. 6 depicts other preferred embodiments of the present invention.Fig. 6 A are the stereochemical structure of reagent strip structure
Schematic diagram, Fig. 6 B are the cross-sectional view of reagent strip structure.In detection means, the carrier material for constituting sample pad 18 is
Glass fibre；The material for constituting label pad is polymer PET 12, and label pad is located on sample reception pad, leans in one end 14 of pad 18
Closed reagent is processed on nearly cellulose membrane；A kind of antibody of the anti-analyte with colored particle is processed in label pad；
The material for constituting analyzed pad 15 is nitrocellulose filter, and the another of specific bond analyte is secured on the film
Plant antibody；With the filter paper for constituting adsorptive pads 17.
Description of symbols：Carrier 100；Specific binding molecule 101, reaction reagent area 106, reaction reagent 102；Closed reagent
103；Closed area 104；Sample applies pad 18；Constitute label pad 12；Sealing pad 14；Analyzed pad 15；Analyte
Specific binding molecule band or detection line (T lines) 11；Adsorptive pads 17.
Specific embodiment
Structure according to the present invention or these technical terms for being used are described further below.
Detection
Detection represents chemical examination, analyzes or tests a kind of material or material whether there is, such as, but be not limited to this, chemistry
Material, organic compound, inorganic compound, metabolism product, medicine or drug metabolite, organic organization or organic organization
Metabolite, nucleic acid, protein or polymer.In addition, detection represents the quantity of test substances or material.Furtherly, detect
It is also represented by immune detection, chemical detection, enzyme detection etc..
Carrier
The carrier for supporting liquid flowing refers to that liquid can flow to another area on carrier from an area.In a tool
In the mode of body, liquid can be processed and tried in reaction from reaction reagent area or closed reagent area stream to analyte land
Reagent in agent area or closed reagent area can be moved on analyzed land by liquid.
On the one hand, the flowing of this liquid can be the different of carrier material itself and allow being moved of liquid active.
In one concrete scheme, device includes a kind of absorbent material, there is provided support the carrier material of liquid flowing." carrier material " is
Refer to a kind of material supported liquid flowing and transport.In a concrete scheme, carrier material is a kind of absorbent material.Liquid flow
Crossing this device is realized by means of capillary motion effect.In different concrete schemes, carrier material can be single material
Bar (Fig. 2), or be made up of various absorbent materials for interacting in a liquid that material is constituted, such as the institutes of Fig. 4 or 6
Show, carrier includes the envelope of label pad 12, the polyester material composition of sample application pad 18, the polyester material composition of glass fibre composition
Close the analyte pad 15 of pad 14, nitrocellulose membrane composition and the adsorptive pads 17 of filter paper composition." water suction " material refers to
Those can stably be absorbed moisture and make moisture be acted on the material of transport by capillary motion wherein.The example of absorbent material
Attached bag includes nitrocellulose, filter paper, glass fibre, polyester and other suitable materials.Meanwhile, this carrier material also includes only carrying
For the carrier of one or several single capillary tubies, liquid is moved by these single capillarities.
On the other hand, the flowing of this liquid also includes passively being moved by the effect of machinery or external force, for example
When liquid is applied on carrier, such as, by plastics, the carrier of the unwetted such as glass material composition allows carrier to incline by external force
Tiltedly, liquid moves to another area from an area under gravity.
In a preferred mode, the reagent processed on carrier is present in a dry state, when liquid is applied to
When on carrier, carrier is moistened by liquid.
Sample applied area
Detection means can include a sample applied area.Sample applied area potentially includes a kind of buffer to dissolve sample
Product, it is also possible to only add the position of sample on a carrier, but be likely to comprising other reagents tested.Example
Such as, those can with reference to sample in disturbing reaction material the useful concrete scheme of " street cleaner " antibody in, " street cleaner "
Antibody can be placed in other areas of sample application area, reagent area or carrier.Therefore sample applied area is also into reaction reagent
Area.Fluid sample is using more convenient when being tested, but it is likely to be dried on test bar, can only add water, buffer and
Other reagents start test with sample dissolution.Sample itself is probably fluid sample, it is also possible to dissolved or be produced
Into liquid solid sample.Sample application area can also include sample application pad 18, when detection, fluid sample be applied
It is added in sample application pad 18.
Reaction reagent
The reagent for completing to react being included in reagent area can be mobile.In specific embodiment, one
A little reagents can be attached on mark substance, and are combined with the target analyte in sample, form the analyte of tape label.
Sample application area and/or reaction reagent area are likely to comprising the sample dissolution needed in special test and adjust the buffering of pH value
Liquid.In a concrete scheme, reagent area includes a species specific binding molecule (for example：A kind of antibody or antibody fragment) and
Mark substance is connected.Mark substance can be any suitable labelling, and such as aurosol, fluorescent dye or water solublity contaminate
Material.The binding molecule of specificity is capable of one or more epitope of specifically combining target analyte, so as to
Labelling analyte.
Reaction reagent area, analyte land
The labelling of combining target analyte provides the detection signal being observed that for generating analyte land,
When analyte is contained in sample, there is mark substance on analyzed calmodulin binding domain CaM.The specific binding molecules of analyte
Carry the labelling of reagent area.When analyte combines the binding molecule capture analyte of the specificity for going and is labeled
Analyte when analyte land is combined, just can see because mark substance gathers here this area
Observe.The binding molecule of the specificity of analyte can also be with the presence for showing analyte in sample or and analyte
The associated molecule of presence combine.Strong bonded refers to combine and reaches change result of the test or make result of the test unobvious
Degree.In some concrete schemes, the binding molecule of specificity be probably a kind of antibody or a kind of antibody fragment (for example,
A kind of Fab areas of antibody), a kind of antigen, a kind of receptor of binding partner or the fragment of receptor, or biotin-streptomycete
Avidin combine to a composition or other types of combination pair.
So reagent area can just provide mark substance, and when sample flows through reagent area, analyte is combined can
To produce the mark substance of detectable signal." mark substance block " is referred on carrier containing can be with reference to there may be in sample
Analyte material position.Therefore a reagent area is exactly a label pad." labelling " can produce can detect
Any suitable mark substance of signal.For example, labelling can be sol particle, fluorescent grain, chemiluminescent molecule, metal
Or alloy (for example, gold colloidal), or capsule, the particularly liposome comprising visible dyes.Hydrophobic sol is also useful, is dredged
Either pigment is insoluble in water or only has very limited sub-fraction solvable for the organic dyestuff of water.Mark substance can also be
Polymer particles, such as coloured granules of polystyrene (for example, spherical).Other useful granular labellings include ferrum egg
In vain, phycoerythrin, phycobilin-albumen, the metal of the either solubility of precipitation or alloy, funguses, Sargassum, or antibacterial
The chlorophyll of pigment or derivant, such as antibacterial or other plant materials.In some concrete schemes, labelling is coloured
Grain, such as dextran bead.
In other concrete schemes, labelling is probably a kind of specific binding with mark substance point of analyte
Sub (a kind of for example, antibody).For example, in a concrete scheme, target analyte is the hepatitis C virus in blood sample
(HCV) with reference to HCV it is, the HCV antigen/antibody combination with aurosol labelling.When sample reaches reagent area (or label pad),
HCV in sample is combined by the HCV antigen/antibody combination of aurosol labelling.Traget antibody is not disturbed to be fixed on analyte land
Another kind of specific binding molecule (capture molecule) and the HCV of labelling combine.For example, labelling can be with reference to the one of analyte
Individual part, and capture molecule can be with reference to another part of analyte or binding marker.HCV- anti-HEV IgGs-gold is multiple
Compound moves to the downstream of carrier.Combine to form gold-HCV-Ab IgG with capture molecule when the complex reaches the analyte binding area
Antibody-HCV- HCV antigen/antibody combination.Capture molecule is probably another kind of specific binding molecules of HCV, or is divided with reference to HCV
The specific binding molecules of the halfbody of analysis thing.When gold-anti-HCV specific binding molecules-HCV- anti-HCV specific binding molecules
When complex is attached to analyte land, analyte land is by the golden marker coloring on complex and analyzed
It is visible that thing land gold labelling becomes naked eyes.In a concrete scheme, the binding molecule of specificity is antibody or antibody piece
Section.Labelling and capture binding molecule can be marked with reference to epitope different on analyte, in a concrete scheme
The specific binding molecules of note combine β-hCG, and capture binding molecule and combine α-hCG.
" antibody " refers to immunoglobulin, either natural or some or all of synthesis.This term is also wrapped
Include the derivant of the antibody for wherein keeping binding ability, also including it is any containing binding domain homologue with immunoglobulin or
The largely protein of homologous binding domain.These protein are derived from natural materials, it is also possible to part or
All synthesize.A kind of antibody is probably monoclonal or polyclone.A kind of antibody is probably any immunoglobulin
A member in type, including the immunoglobulin class of any mankind：IgG, IgM, IgA, IgD, IgG and IgE." antibody fragment "
It is derivant or a part less than total length of antibody of antibody.Antibody fragment can retain at least one full length antibody
The notable site of binding ability.The example of antibody fragment includes Fab, Fab', F (ab')2, scFv, Fv, dsFv dimer, and Fd
Fragment, but not only include the above.
Antibody fragment can be generated by any mode.For example, antibody fragment can be by enzymolysis or chemical cracking one
Complete antibody, or can also be by the gene recombinaton from coded portion antibody sequence generating.In other words, antibody fragment
Can partially or entirely be recombinated generation.Antibody fragment can be arbitrary single chain antibody fragments.In other words, antibody piece
Section can include a plurality of peptide chain for interconnecting, for example, by disulfide linkages.Antibody fragment can also be arbitrary one kind
Multi-molecular complex.One functional antibody fragment generally comprises at least about 50 aminoacid, and more antibody fragments
Generally comprise at least about 200 aminoacid.
ScFv s (scFvs) is the antibody fragment of restructuring, and it is only by variable light (VL) and variable heavy chain (VH) mutually with
Polypeptide chain covalent bond.VLAnd VHIn a side there are amido end regions.Polypeptide chain length and composition are variable, and its length can be with
Make the mutual bridging of two variable domains and the arrangement to atom does not have a strong impact on.Polypeptide chain is generally main by glycine and silk ammonia
Sour residue extends composition, and some of which glutamic acid and lysine residue are dispersed in distribution to increase its dissolubility." dimer " is
Refer to the dimer of scFv s.The peptide chain that dimeric monomer is included is generally short than most of scFv s, and they show
Go out to form the tendency of dimer.
" Fv " fragment is by a VHWith a VLDomain is connected with each other composition with non-covalent.Term " dsFv " herein refers to bag
Containing a stable VH-VLTo intermolecular disulfide bond Fv.“F(ab')2" fragment be antibody a fragment, substantially and use stomach
It is identical that protease digests the fragment that immunoglobulin (typically IgG) obtains in pH 4.0-4.5.This fragment can also be again
It is combined into." Fab' " fragment is a kind of antibody fragment, substantially and by reducing F (ab')2Two heavy chains in fragment are mutually interconnected
The fragment that the cystine linkage for connecing is obtained is identical.Fab' fragments can also be re-combined into." Fab " fragment is a kind of substantially and to use Fructus Chaenomeliss
The fragment identical antibody fragment that protease digestion immunoglobulin (usual IgG) is obtained.Fab fragments can also be re-combined into.
Heavy chain fragment in Fab fragments is Fd fragments.
Closed reagent closed reagent area
The closed reagent for being included in closed reagent area is moveable.It is any will not be between materially affect specific binding molecule
The reagent of association reaction can serve as closed reagent, such as some inert proteins, including casein or albumin, such as cattle
Serum albumin.It is also the common technological means in this area technology of the closed reagent process on carrier, such as closing examination
Agent is configured to solution and then soaks carrier, then carrier drying.It is of course also possible to add other reagents in closed reagent
To improve the solubility property or the performance with reference to carrier of closed reagent, for example, casein is dissolved in pH value and is delayed for 7-9.5Tris
In rushing solution.When liquid is through closed reagent area, be processed at closed reagent in the area by liquid moistening then with
Liquid is moved together to analyte calmodulin binding domain CaM.In a specific example, in the case of moistening, closed reagent prior to
Specific binding molecule on reaction reagent contact analyte land.In another specific example, in detection means
When each area is to do, when detection is needed, fluid sample is applied on carrier sequentially wet by each area in device
Profit, then completes detection.In the case of this moistening, we have surprisingly found that closed reagent to the hole on carrier or special
Site on binding molecule has good sealing effect.
Closed reagent is allowed to have various prior to the mode that reaction reagent contacts the specific binding molecule on analyte land.
In a specific mode, such as shown in Fig. 5, enclosed area 104 and reaction reagent area 106 is allowed to be located at analyte
The both sides of land 105, the distance of the closed reagent 103 allowed on enclosed area and specific binding molecule 101 less than reaction reagent with
The distance of specific binding molecule, here, reaction reagent 102 includes a kind of antibody of purpose analyte, analyte land
Specific binding molecule 101 on 105 includes another kind of antibody of purpose analyte, and the antibody is fixed on land 105.
Reaction reagent and closed reagent are also processed on carrier, and they can move with fluid sample.When detection, to reaction
Apply fluid sample on reagent area 106, allow reaction reagent to move to specific binding molecule 101 with fluid sample, meanwhile, to closing
Area 104 apply fluid sample, allow closed reagent to move to specific binding molecule 101 with fluid sample, when the material of carrier it is the same
When, closed reagent can prior to reaction reagent reach analyte calmodulin binding domain CaM 105 and with specific binding molecule 101 on one
A little sites combine.The site being closed is probably some nonspecific sites on specific binding molecule.Subsequently, reaction reagent is reached
On analyte specific binding molecule 101, due to some non-specific sites it is combined after, if there is no purpose quilt in sample
Analysis material, reaction reagent would not occur specific bond with specific binding molecule, allow testing result to be accurate negative findings.
In addition to upper type, the sequencing for applying sample can also be changed by closed reagent prior to reaction reagent contact analyte
Specific binding molecule on land.For example, first apply sample and apply sample again on enclosed area 103, then to reaction reagent
In area 106；Or directly apply micro closed reagent solution on analyte calmodulin binding domain CaM, such as 1-10 microlitre, then
Apply fluid sample on reaction reagent takes again.
In another specific mode, closed reagent area 105 and reaction reagent area 106 is allowed to be all located at analyzed land
105 upstream, meanwhile, allow closed reagent area to be located between reaction reagent area and analyzed land, as shown in Figure 3.Carry out
Before test, carrier is dry state；When fluid sample being applied in reaction reagent area, fluid sample connects first with reaction reagent
Touch and drive reaction reagent to move to analyte land, because reaction reagent is present in a dry state in advance reaction reagent
Qu Shang, needs the about a bit of time, about 5-20 second, allows these dry reagents to be dissolved or be moistened by fluid sample.Here
During, due to the part moisture movement speed in fluid sample, can contact in advance positioned at the closed area in downstream
And moistening closed reagent thereon, allow closed reagent to be faster than reaction reagent and move to analyte calmodulin binding domain CaM.Move in advance
Partially enclosed reagent through analyte calmodulin binding domain CaM, on the one hand may be pointed to closed reagent and analyte land in advance
Carrier between domain, such as nitrocellulose filter, carry out advance process, are on the other hand likely to close special on the region in advance
Some non-Non-specific binding sites on different binding molecule, in addition, it is also possible to neutralize specific binding molecule on analyzed land
Electric charge etc..So, subsequent reaction reagent is allowed, such as the antibody or fragment of the specific bond analyte with mark substance
With the specific binding molecule on analyte specific binding region, another antibody or fragment of such as specific bond analyte are carried out
The combination of specificity, reduces nonspecific combination, improves the accuracy of detection.
In another mode, constitute detection means is nitrocellulose assay strip, including fluid sample applies pad
18, a label pad 12 is set in sample pad and label pad is allowed away from closed reagent；The one end 14 for applying pad in sample is processed
There is closed reagent.With a nitrocellulose filter 15, process what specific binding molecule was connected with 16 and with thin film 15 on thin film
Filter paper 17, Fig. 6 B represent the generalized section of reagent strip；Fig. 6 A represent the dimensional structure diagram of reagent strip.When fluid sample
It is applied on applying pad, partially liq sample is flowed directly on closed reagent region 14 along the direction of arrow, and another part liquid
Body sample needs first to moisten label pad 14, moistens the dry specific bond with colored particle processed in advance in label pad point
Son；Allow closed reagent under humidified condition prior to the reagent contact quilt in label pad 14 because the path of liquid flow direction is different
Specific binding molecule on analysis calmodulin binding domain CaM.
Fig. 2 is a specific embodiment of the present invention, and Fig. 2A is the vertical view for not applying the detection means before fluid sample
Structural representation；Fig. 2 B are the overlooking the structure diagram for applying the detection means after fluid sample.In detection means, including one
Individual single carrier 100, includes analyte land 105 on 100, and a species specific binding molecule is included on 105
101, and a reaction reagent area 106, another for including the specific bond analyte with mark substance on 106 is special
Binding molecule；Include closed reagent 103 with a closed area 104,104；Analyte land and reaction reagent area are by cloth
The upstream and downstream in same liquid flow direction is put, and enclosed area 104 is configured to (Fig. 2A) vertical with analyte land,
Reaction reagent area 106 is allowed to be more than closed reagent area 104 and analyte land with the distance of analyte land 105 simultaneously
105 distance, so allows flowing time of the fluid in two intervals different, so as to the time for exhibiting up to land 105 has
Successively.When being detected, fluid sample is applied on carrier 100, allows it from reaction reagent region to analyte
Land is moved, and fluid sample is applied on carrier 104 in addition, allows liquid to move to analyte land along carrier 104
Dynamic, such closed reagent takes analyte calmodulin binding domain CaM to and first combines some sites on specific binding molecule 101 by liquid,
In the case of moistening, liquid arrives soon after specific bond area 105 with the reagent in reagent areas, if exist in sample divided
Analysis material, the accumulation mark substance on analyte calmodulin binding domain CaM, so as to by calculate mark substance accumulation number judging
The number of analyte.
Fig. 4 depicts another preferred embodiment of the present invention.Fig. 4 A are the stereochemical structure of reagent strip structure
Schematic diagram, Fig. 4 B are the overlooking the structure diagram of reagent strip structure.In detection means, the carrier material that sample applies pad 18 is constituted
Expect for glass fibre；The material for constituting label pad is polymer PET 12, and anti-third type having with latex particle is processed in label pad
The first Monclone antibody of hepatitis viruss；The material for constituting sealing pad is polymer PET 14, and processing on the film has casein；Structure
Material into analyzed pad 15 is nitrocellulose filter, and the another of specific bond hepatitis C viruss is secured on the film
Plant Monclone antibody 11；With constitute adsorptive pads 17 filter paper, they join end to end, allow fluid sample can from sample pad 18 flow to
On adsorptive pads 17.
" prior to " in specific binding molecule on closed reagent " prior to " reaction reagent contact analyte land exists
The present invention can be expressed as shifting to an earlier date, ahead of time, earlier than.There may be reaction reagent contact of the closed reagent of 1-99% prior to 1-99%
The specific binding molecule of the 1-99% on analyte land；The closed reagent of 10-99% is likely to prior to 10-90%'s
The specific binding molecule of the 20-99% on reaction reagent contact analyte land；There may also be the closed reagent of 50-99%
The specific binding molecule of the 10-80% on reaction reagent contact analyte land prior to 30-80%.
Fig. 1 is the overlooking the structure diagram of reagent strip in conventional apparatus, and Figure 1A is not apply the dress of the detection before fluid sample
The overlooking the structure diagram put；1B is the overlooking the structure diagram for applying the detection means after fluid sample.In detection means,
Including a carrier 100, analyte land 105 is included on 100, including a kind of special analyte on 105
Binding molecule 101, generally, before detection, processing on analyte land 10 in advance has closed reagent and is dried；
With a reaction reagent area 106, another specific bond of the specific bond analyte with mark substance is included on 106
Molecule；Analyte land and reaction reagent area are disposed in the upstream and downstream of same liquid flow direction；This reagent strip
For the reagent strip done.When being detected, fluid sample is applied on carrier 100, allow its from reaction reagent region to
Analyte land is moved, and in the case of moistening, liquid reaches specific bond area 105 with the reagent in reagent areas,
If there is analyte in sample, the accumulation mark substance on analyte calmodulin binding domain CaM, so as to pass through to calculate label
Matter accumulation number come judge analyte number.
Detection method
On the other hand, the method that the present invention also provides analyte in a kind of analysis fluid sample, including：There is provided a kind of
The carrier 100 of liquid flowing is supported, is included on the carrier：One analyte land 105, includes one on described land
Plant specific binding molecule 101；One or more complete the reaction reagent 102 for reacting；With one or more closed reagents 103；Allow
Detectable and closed reagent are as liquid is to the specific binding molecule movement on analyte land；Closed reagent is allowed than anti-
Reagent is answered to contact the specific binding molecule on described analyte land in advance.It is analyzed in a specific mode
Specific binding molecules include a kind of antibody or antibody fragment of specific bond analyte on thing land, and reaction reagent includes
Another kind of antibody of specific bond analyte or fragment.In a concrete mode, closed reagent includes and analyte
The reagent that site on land on specific binding molecules combines.
Compared with traditional technology, this device or method of the present invention can well improve the accuracy of detection, its
Reason is probably：Under wetness conditions, closed reagent has preferably closing to the site on some molecules or the hole on carrier
Effect, while and to carrier not producing some detrimental effects in itself.Because in traditional sealing technique, needing first carrier
In being immersed in lock solution, then carrier is dried again, in such processing procedure, some tables that may be allowed on carrier
Face active agent is closed eluant solution and falls, so as to have impact on the performance of carrier.In addition, the closed reagent in apparatus of the present invention can
With negatively charged in the case of moistening, the specific binding molecule with positive charge on analyzed land can be in advance contacted,
The positive charge on special molecular has been neutralized, nonspecific combination of specific binding molecule and reaction reagent has been reduced.Especially, when
When the antibody of gold grain labelling is included in reaction reagent, it often carries negative charge, special on analyte land
The electric charge of binding molecule is closed in reagent with after the combination reduced to gold grain antibody, so as to improve the accurate of detection
Property.
The type of sample
Any kind of sample can be tested with the device of the present invention, including body fluid (for example, urine and other bodies
Liquid, and clinical sample).Fluid sample may originate from solid or semisolid sample, including excrement, biological tissue and food
Sample.These solids and semisolid sample fluid sample can be transformed into by any suitable method, such as in one kind
In appropriate liquid mix, stamp it is broken, macerate, incubation, dissolving or digest solid sample (for example, water, phosphate buffer or
Other buffer)." biological sample " includes the sample of the animal, plant and food for being derived from living, also including urine, saliva, blood
With blood constituent, cerebrospinal fluid, vaginal swab, seminal fluid, feces, perspiration, secretions, tissue, organ, tumor, tissue and organ
It is the conditioned media of culture, cell culture and there, either people or animal.Foodstuff samples include finished food
Thing composition and last product, meat, cheese, wine, milk and drinking water.Plant sample include be derived from any plant, plant tissue,
The sample of the conditioned media of plant cell cultures and there." environmental sample " is sample (for example, the lake water that those are derived from environment
The sample of sample or other water bodys, sewage sample, pedotheque, groundwater sample, seawater sample, the sample of runoff water).
The waste of sewage and correlation can also be included in environmental sample.
The type of analyte
Any analyte can be analyzed with the present invention.Can be with the example bag of the analyte of stable detection of the present invention
Include (but not only including) human chorionic gonadotropin (hCG), lutropin (LH), ovarian stimulation element (FSH), hepatitis C
The medicine of viral (HCV), hepatitis B virus (HBV), hepatitis B surface antigen, HIV (human immunodeficiency virus) and any abuse.Analyte can
Detect in any liquid or liquefied sample, such as urine, saliva, saliva, blood, blood plasma, or serum.It is other
The example of analyte also has the acid of flesh ammonia acid anhydride, bilirubin, nitrite, protein (nonspecific), blood, leukocyte, blood
Sugar, heavy metal and toxin, bacterial component (for example, the special protein and sugar of certain types of antibacterial, such as escherichia coli
0157:H7, staphylococcus aureuses, Salmonella, bacillus perfringens, Campylobacter, listeria monocytogenes, enteritis arc
Bacterium, or wax bacillus cereuss).The analyte of any other suitable lateral flow test format can be detected with this device.
Test
Application of the closed reagent in gold colloidal lateral chromatography diagnosis test paper
The following examples will be further illustrated the present invention, but should not be construed as the limit to the scope of the invention anyway
System.The structure of the reagent strip that this test is used and the preparation of associated materials and reagent are illustrated with reference to Figure of description 4.
1. test material
1.1 protein raw materials and material
| Material | Lot number | Expiration date |
| HCV antigens cs 66 (gold mark labelling) | F090609HCV-1 | 2009.10.15 |
| HCV antigens cs 55 | 090527 | 2009.10.27 |
| Tris salt buffer solutions | 9T91JA | 2011.07.07 |
| Casein Casein | 048K0067 | 2011.10.14 |
| Borax salt buffer solutions | 048K0009 | 2013.02.21 |
| Surfactant S19 | F20080110 | 2010.01.10 |
| NaCl | 108K0051 | 2014.09.30 |
| Na2PH4 | 097K0022 | 2010.08.21 |
| BSA | BAH62-900 | 2014.02.28 |
| Glass fibre | 4072402 | 2011.06.03 |
| Millipore NC (nitrocellulose membrane) | R6MN95421 | 2009.12.10 |
1.2 for examination positive serum and negative serum
2. experimental technique
2.1 preparations for processing the solution of sealing pad 14：
Tris: 2.5mg/ml
Casein: 2.5mg/ml
PH=8.0
2.2 preparations for processing the solution of gold standard pad 12：
Tris: 2.5mg/ml
BSA: 10.0mg/ml
PH=8.0
2.3 preparations for processing the solution of sample pad 18：
Borax: 2.5mg/ml
Casein: 2.5mg/ml
S19:0.5%
PH=8.0
2.4 preparations for processing the solution of T lines 11 on NC films 15：
NaCl: 8.8mg/ml
Na2PH4: 2.1mg/ml
PH=7.4
The process of 2.5 gold standard pads 12
With the labeled HCV antigens cs 66 of gold standard pad solution dilution gold grain (for our company from line flag, antigen is from depth
Zhen Fei rocs company buys), final concentration is allowed to OD3.5.Gold 1.5 milliliters of the treatment fluid of mark for having diluted uniformly sprays glass fibre
On (length × wide=0.8cm × 30.1cm), it is placed in overnight being dried in 37 DEG C of drying baker, is then cut into length × wide=0.8cm
The small pieces of × 0.6cm, as label pad g.
The process of 2.6 sample pad 18 and sealing pad 14
It is sprayed directly on glass fibre with isopyknic sample pad or sealing pad solution respectively, the such as table of the reagent in solution
Carrying out listed by 1 is processed (length of sample pad × wide=1.8cm × 0.6cm, the length × wide=0.5cm × 0.6cm of sealing pad), place
It is placed in overnight being dried in 37 DEG C of drying baker after reason.
The process of the sample pad of table 1 and sealing pad
| Process | Tris | Casein | S19 | PH |
| Sample pad a | 2.5mg/ml | 2.5mg/ml | 0.5% | 8.0 |
| Sample pad b | 2.5mg/ml | Nothing | 0.5% | 8.0 |
| Sealing pad c | 2.5mg/ml | Nothing | 0.5% | 8.0 |
| Sealing pad d | 2.55mg/ml | 2.5mg/ml | 0.5% | 8.0 |
The process of 2.7T lines
The solution (antigen is voluntarily produced for company) of T lines solution dilution HCV antigens cs 55, and with 1.0ul/cm, discharge rate spray
On NC films 15 (a width of 2.5 centimetres), NC film e are designated as.And be placed in 37 DEG C of drying baker and overnight dry.
2.8 traditional membrane closures
With on sealing pad processing solution 1ml even applications to the NC films (a width of 2.5 centimetres) for processing T lines, then it is put into
Overnight dry in 37 DEG C of drying baker, as NC film f.
3. the assembling of reagent strip
According to structure and order shown in Fig. 4, to sample pad 18, label pad 12, the (if there is) of sealing pad 14 and NC films 15,
And filter paper pads 17 are assembled, allow sample pad 18 to be superimposed upon in label pad 12, allow label pad 12 to be superimposed upon on sealing pad 14, allow
Sealing pad 14 is superimposed upon on NC films 15, allows filter paper 17 to be superimposed upon on NC films, allow reagent strip width be 0.6 centimetre；If no
The reagent strip of sealing pad, when assembling, allows label pad 12 to be directly superimposed upon on NC films 15.
4. operational approach
Then the drop of Deca 2 was with the naked eye seen for examination positive serum or negative serum in 5 minutes in the sample pad of reagent strip
The shade on T lines is examined, and ("+" represents positive, and "-" represents cloudy to be compared to interpretation testing result with the colour atla of standard
Property).
The colour atla standard of table 2 and testing result
Test 1 compare Casein in sample pad to eliminating false-positive effect
Process
Experimental result
| H | M2 | N1 | N2 | N3 | N4 | N5 | N6 | N7 | |
| Reagent strip 1 | 6+ | +5 | +3.5 | +4 | +3.6 | +3.5 | +4 | +4 | +3.5 |
| Reagent strip 2 | 7+ | +6 | +6 | +6 | +6 | +6 | +7 | +6 | +6 |
As can be seen from the above results, after process has casein reagent in sample pad, do not interfere with to positive sample
The detection of accuracy, and when negative sample is detected, although the false-positive degree of generation can be mitigated, i.e., on T lines
Color depth is significantly reduced, but signal reaction remains as the positive.
Experiment 2 is compared same concentration C asein and false-positive effect is eliminated in sealing pad and sample pad
Process
Result of the test
| H | M2 | N1 | N2 | N3 | N4 | N5 | N6 | N7 | |
| Reagent strip 1 | +6 | +5 | +3.5 | +4 | +3.6 | +3.5 | +4 | +4 | +3.5 |
| Reagent strip 3 | +6 | +5 | -1 | -1 | -2.5 | -1 | -2 | -1 | -1 |
| Reagent strip 4 | +6 | +5 | -1 | -1 | -2 | -1 | -2 | -1 | -1 |
Can be seen that from above experimental result and no matter whether processed casein in sample pad, processing on sealing pad has
Caseic reagent strip can significantly eliminate the false positive results of negative sample, make testing result more accurate；Not simultaneously
Affect the detection accuracy of positive sample.
Experiment 3 verifies whether the sealing pad without Casein equally has and eliminates false-positive effect
Process
Experimental result
| H | M2 | N1 | N2 | N3 | N4 | N5 | N6 | N7 | |
| Reagent strip 5 | +6 | +5 | +4 | +4 | +5 | +4 | +4 | +4 | +4 |
| Reagent strip 1 | +6 | +5 | +3.5 | +4 | +3.6 | +3.5 | +4 | +4 | +3.5 |
| Reagent strip 4 | +6 | +5 | -1 | -1 | -2 | -1 | -2 | -1 | -1 |
As can be seen from the above results, when there is no sealing pad or there is sealing pad but when without closed reagent, still
So do not make significant difference to reducing HCV false positives；On the contrary, when process has closed reagent caseic on sealing pad, can disappear
Except the false positive test results that ' negative ' specimens cause.
Sealing pad closing method is compared in experiment 4 and membrane closure method eliminates false sun effect.
Process
Experimental result
| H | M2 | N8 | N9 | N10 | N11 | N12 | |
| Reagent strip 3 | +5 | +4 | -2 | -1 | -2 | -1 | -2 |
| Reagent strip 6 | +7 | +5 | -2 | -1 | -2.5 | -1 | -1 |
| Reagent strip 7 | +9 | +6 | +6 | +6 | +6 | +7 | +6 |
From the point of view of the result of test 4, sealing pad closing method and spraying membrane closure method find two to eliminating false-positive effect
Person's effect is similar, while, it was found that using the method for tradition closing, there is large effect to positive sensitivity.But utilize
Sealing pad closes method, and the impact to sensitivity less, and can simplify production technology, reduce production cost, improve production efficiency.
Claims (12)
1. a kind of detection means, including:The carrier of liquid flowing is supported, is included on carrier:One analyte land, one
Individual reaction reagent area；One closed reagent area；Described analyte land includes a species specific binding molecule；It is described
Closed reagent area on include one or more closed reagents；Reaction is completed in described reaction reagent area including one or more
Reaction reagent；Wherein, the reaction reagent area is more than closed reagent area and analyte with the distance of analyte land
The distance of land；Wherein, reaction reagent area is located at the upstream of analyte land, closed reagent area and reaction reagent area and
The direction of analyte land arrangement is vertical.
2. device as claimed in claim 1, wherein, between the specific binding molecule on closed reagent and analyte land
Distance less than the distance between specific binding molecule on reaction reagent and analyte land.
3. device as claimed in claim 1, wherein, described reaction reagent includes the molecule of specific bond analyte；Quilt
Specific binding molecules on analyte land can be on association reaction reagent area specific bond analyte molecule.
4. device as claimed in claim 3, wherein, reaction reagent is also included with coloured marking particle.
5. device as claimed in claim 3, wherein, analyte land is located on nitrocellulose filter.
6. device as claimed in claim 3, wherein, described closed reagent and reaction reagent is combined with liquid to analyte
Move in area.
7. device as claimed in claim 1, wherein, reaction reagent includes the specific bond analyte being connected with colored particle
Molecule；The molecule of specific bond analyte of the closed reagent ratio with colored particle contacts in advance the analyte and combines
Specific binding molecules in area.
8. device as claimed in claim 7, wherein, the specific binding molecule on analyte land is the of analyte
One antigen, the molecule of the specific bond analyte with colored particle is the second antigen of analyte.
9. device as claimed in claim 8, wherein, the specific binding molecule specific bond on analyte land carries face
The molecule of the specific bond analyte of coloured particles.
10. the device as described in one of claim 1-9, wherein, the combination point of the specificity on the analyte land
Son is fixed on carrier.
11. devices as described in one of claim 1-9, wherein, described closed reagent is in serum albumin or casein
One or two.
12. devices as described in one of claim 1-9, wherein, described closed reagent combines non-on specific binding molecule
Specific binding site.
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| CN106290865A (en) * | 2016-08-12 | 2017-01-04 | 山西中嘉加泰生物科技有限公司 | Salmonella infects colloidal gold immunochromatographimethod 5-linked diagnostic kit |
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| CN2445326Y (en) * | 2000-10-09 | 2001-08-29 | 刘永详 | Immune analysis device for assaying saccharified protein |
| US20030129680A1 (en) * | 2001-10-31 | 2003-07-10 | O'connor Thomas Patrick | Multi-analyte assay device |
| FR2853077B1 (en) * | 2003-03-28 | 2005-12-30 | Vedalab | SOLID PHASE IMMUNOCHROMATOGRAPHIC METHODS |
| US7344893B2 (en) * | 2005-10-13 | 2008-03-18 | Auric Enterprises, Llc | Immuno-gold lateral flow assay |
| CN101017169B (en) * | 2006-07-26 | 2012-07-18 | 艾博生物医药(杭州)有限公司 | Analysis equipment of biological sample |
| CN101435823B (en) * | 2007-11-12 | 2012-08-08 | 无锡中德伯尔生物技术有限公司 | Fluorescent micro-ball immune chromatography test paper strip for detecting residual animal medicine and preparing method thereof |
| WO2009070812A1 (en) * | 2007-11-29 | 2009-06-04 | Inverness Medical Switzerland Gmbh | Assay |
| CN101509922A (en) * | 2009-03-24 | 2009-08-19 | 南通市伊士生物技术有限责任公司 | Vitro diagnosis detecting test paper and method for making same |
| CN102169120B (en) * | 2010-02-25 | 2014-09-17 | 艾博生物医药(杭州)有限公司 | Detector |
| CN102236014A (en) * | 2010-04-20 | 2011-11-09 | 艾博生物医药(杭州)有限公司 | Detection reagent strip based on immunoreaction principle |
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