CN105136704B - A kind of method of quick measure brewer's yeast milk dry matter content - Google Patents
A kind of method of quick measure brewer's yeast milk dry matter content Download PDFInfo
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- CN105136704B CN105136704B CN201510582594.6A CN201510582594A CN105136704B CN 105136704 B CN105136704 B CN 105136704B CN 201510582594 A CN201510582594 A CN 201510582594A CN 105136704 B CN105136704 B CN 105136704B
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Abstract
The present invention relates to a kind of method of quick measure brewer's yeast milk dry matter content, belong to biological yeast detection technical field.Technical scheme is：Yeast milk 1g is weighed as sample, is settled to 250ml；After obtaining 50 sample above at random, measure solution absorbance value A respectively under 600nm wavelength conditions, then sample dry matter content is detected with national standard method；Model foundation is carried out, obtains the standard curve between absorbance A and dry matter content；Take brewer's yeast in actual production to detect sample, detect the solution absorbance value of sample, the dry matter content of the detection sample is tried to achieve on standard curve, you can obtain the dry matter content of brewer's yeast breast in actual production.The present invention is applied to small business, it is not necessary to buys the analytical instrument of costliness, rapid and accurate determination brewer's yeast milk dry matter, in time data are provided for the enzyme-added enzymolysis of technique of next step, the time is saved for actual production, while avoids national standard method from autolysis occur because of detection time length, yeast milk.
Description
Technical field
The present invention relates to a kind of method of quick measure brewer's yeast milk dry matter content, belong to biological yeast detection technology
Field.
Background technology
Brewer's yeast has abundant nutriment, and the methods of passing through enzymolysis can be by its intracellular protein degradation
Into amino acid and polypeptide, nucleolysis is into nucleotides, and together with intracellular other active ingredients, such as B family vitamin, glutathione
And trace element etc. extracts from yeast cells together, is processed further can obtain yeast extract.It is that one kind has concurrently
Seasoning, the natural nutritious flavouring material of nutrition and health care three zones.China is Beer Brewage state maximum in the world, and annual production is
Surpass 50,000,000 tons, 1,000,000 tons or so of brewer's yeast can be produced.Therefore, strengthen the integrated application to brewer's yeast, improve product
Added value, not only with the wide market space, meanwhile, can reduce waste discharge, strengthen environmental protection, produce huge warp
Benefit of helping and social benefit.In GB/T 23530-2009《Yeast extract》In, dry pushes away solid content using moisture evaporation is counter
The method of content, detection time at least need a few hours, are not suitable for the control of critical process in production process；Quick moisture is surveyed
Determine instrument and only determine solid or the sample dry matter of pulverulence, can not be detected for liquid sample；And near infrared spectrometer etc.
Though analytical instrument can simple, quick determination sample dry, instrument cost is very high, generally more than 500,000 RMB, no
It is applicable some small business.
The content of the invention
It is an object of the present invention to provide a kind of method of quick measure brewer's yeast milk dry matter content, suitable for small-sized enterprise
Industry, it is not necessary to buy the analytical instrument of costliness, rapid and accurate determination brewer's yeast milk dry matter, add in time for the technique of next step
Enzyme enzymolysis provides data, and the time is saved for actual production, while avoids national standard method from self-dissolving occur because of detection time length, yeast milk
Phenomenon, solves above mentioned problem present in background technology.
The technical scheme is that：
A kind of method of quick measure brewer's yeast milk dry matter content, includes following processing step：
1. beer yeast slurry is cleaned, cleaned, brewer's yeast breast is obtained after separation；
2. weighing yeast milk 1g as sample, pure water dissolving, 250ml, referred to as sample are settled to；
3. at random obtain 50 sample above after, using pure water as blank, detected respectively under 600nm wavelength conditions 50 with
The solution absorbance value A of upper sample, obtain the absorbance of each sample；Then the dry with national standard method to 50 samples
Matter content is detected, and obtains the dry matter content of each sample；
4. model foundation is carried out, using the absorbance A of each sample of acquisition as abscissa, with each sample of acquisition
This dry matter content is ordinate, obtains the standard curve between absorbance A and dry matter content；
5. the measure of brewer's yeast milk dry matter content in actual production, beer yeast slurry in actual production is taken to clean, be clear
Wash, brewer's yeast breast is obtained after separation, weigh yeast milk 1g as detection sample, pure water dissolving, be settled to 250ml, referred to as examine
Test sample sheet, using pure water as blank, the solution absorbance value A of the detection sample is measured under 600nm wavelength conditions, in standard curve
On try to achieve the dry matter content of the detection sample, you can obtain the dry matter content of brewer's yeast breast in actual production.
Shown by testing detection：Sample of the present invention is settled to 250mL, and the testing result of absorbance is in 0.2~0.7 model
In enclosing, belong to the most sensitive volume of spectrophotometer；The method according to the invention, sample and difference to gathering identical source
The sample standard deviation in source carries out model prediction, provable predicted value and actual value error very little, demonstrates the accurate of the inventive method
Property.By carrying out analysis contrast to the repeated detection result of same sample of the present invention, error is in allowed band, the inventive method weight
Renaturation is good, and the degree of accuracy is high.
The present invention innovative point be：It is determined that most rational sample（It is settled to 250ml）, be the invention main points it
One；Founding mathematical models, brewer's yeast milk dry matter content in actual production is quick and precisely detected, is the invention main points
Two；Suitable for small business, it is not necessary to buy the analytical instrument of costliness, provide number in time for the enzyme-added enzymolysis of technique of next step
According to, for actual production save the time, be the invention third key point.
The beneficial effects of the invention are as follows：Suitable for small business, it is not necessary to buy the analytical instrument of costliness, quick and precisely survey
Determine brewer's yeast milk dry matter, provide data for the enzyme-added enzymolysis of technique of next step in time, save the time for actual production, simultaneously
Avoiding national standard method, there is autolysis in yeast milk because detection time is grown.
Brief description of the drawings
Fig. 1 is the model that the embodiment of the present invention quickly determines brewer's yeast milk dry matter content；
Regression equation：Y=22.114X-0.1779；Wherein X-absorbance A, Y-dry matter content；
R2=0.9991；
Fig. 2 is dry matter content predicted value probability graph of the present invention collection from identical self-dissolving tank；
Fig. 3 is dry matter content error amount probability graph of the present invention collection from identical self-dissolving tank；
Fig. 4 is dry matter content fast prediction value probability graph of the present invention collection from different self-dissolving tanks；
Fig. 5 is dry matter content error amount probability graph of the present invention collection from different self-dissolving tanks.
Embodiment
Below in conjunction with accompanying drawing, by example, the invention will be further described.
A kind of method of quick measure brewer's yeast milk dry matter content, includes following processing step：
1. beer yeast slurry is cleaned, cleaned, brewer's yeast breast is obtained after separation；
2. weighing yeast milk 1g as sample, pure water dissolving, 250ml, referred to as sample are settled to；
3. after obtaining 50 samples at random, using pure water as blank, detect 50 samples respectively under 600nm wavelength conditions
Solution absorbance value A, obtain the absorbance of each sample；Then the dry matter content of 50 samples is entered with national standard method
Row detection, obtain the dry matter content of each sample；
4. model foundation is carried out, using the absorbance A of each sample of acquisition as abscissa, with each sample of acquisition
This dry matter content is ordinate, obtains the standard curve between absorbance A and dry matter content, referring to the drawings 1；
5. the measure of brewer's yeast milk dry matter content in actual production, beer yeast slurry in actual production is taken to clean, be clear
Wash, brewer's yeast breast is obtained after separation, weigh yeast milk 1g as detection sample, pure water dissolving, be settled to 250ml, referred to as examine
Test sample sheet, using pure water as blank, the solution absorbance value A of the detection sample is measured under 600nm wavelength conditions, in standard curve
On try to achieve the dry matter content of the detection sample, you can obtain the dry matter content of brewer's yeast breast in actual production.
The present embodiment obtains sample size as 50 at random.
The technical characteristic " weighing yeast milk 1g as sample, pure water dissolving, be settled to 250ml, referred to as sample " of the present invention,
With unexpected technique effect, above-mentioned conclusion is drawn by experiment.Specific experiment process：5 samples are chosen, each
Sample weighs 1g yeast milks, is settled to different volumes respectively, 50-500 milliliters, to each sample respectively under 600nm visible rays
Absorbance A is detected, measurement three times is averaged, as a result as shown in table 1.
Table 1
Note："-" is beyond instrument range ability.
As shown in Table 1, when being settled to 250mL, the testing result of absorbance belongs to light splitting light in the range of 0.2~0.7
Spend the most sensitive volume of meter.
In the embodiment of the present invention, 50 samples are handled and detected according to the method for the present invention, dry and absorbance
Value A measurement result is as shown in table 2.
Table 2
By table 2, to draw using absorbance A as abscissa, dry matter content is the scatter diagram of ordinate, adds Trendline,
Try to achieve regression equation and R2.Referring to the drawings 1.
The equation of the standard curve is：Y=22.114X-0.1779；Wherein X-absorbance A, Y-dry matter content；
R2It is the coefficient of determination（coefficient of determination）, or be coefficient of determination, the goodness of fit, work as R2Closer to 1
When, represent that related equation reference value is higher；R of the present invention2=0.9991。
The sample standard deviation of sample and separate sources to gathering identical source carries out model prediction, provable predicted value and reality
Actual value error very little, demonstrate the accuracy of the inventive method.
First, Parallel testing three times is carried out to same sample, corresponding dry matter content is obtained through regression equation calculation, while with
The numerical value that national standard detection method obtains is contrasted, and its result is as shown in table 3：
Table 3
As shown in Table 3, Parallel testing is carried out by the method, the testing result error of same sample is in allowed band, side
Method is reproducible, and the degree of accuracy is high.
2nd, 10 samples from identical self-dissolving tank are gathered with the detection of national standard detection method in the process of the present invention respectively, as a result
As shown in table 4：
Table 4
Validation of Data：
Using MINITAB statistical analysis softwares, the error amount that predicted value and predicted value and national standard are detected to actual value divides
The confidential interval figure of normal state -95 is not done, referring to the drawings 2, accompanying drawing 3, from confidential interval it can be seen that predicted value and error amount are 95%
In confidential interval, data acquired by explanation are acceptable error scope.
3rd, to gathering from different self-dissolving tanks（4#-14# tanks）Yeast milk carry out Parallel testing three times respectively, through recurrence side
Journey calculates to obtain corresponding dry matter content, while the numerical value obtained with national standard detection method is contrasted, its result such as institute of table 5
Show：
Table 5
Validation of Data：
Using MINITAB statistical analysis softwares, the error amount that predicted value and predicted value and national standard are detected to actual value divides
The confidential interval figure of normal state -95 is not done, referring to the drawings 4, accompanying drawing 5, from confidential interval it can be seen that predicted value and error amount are 95%
In confidential interval, data acquired by explanation are acceptable error scope.
Claims (2)
- A kind of 1. method of quick measure brewer's yeast milk dry matter, it is characterised in that include following processing step：1. beer yeast slurry is cleaned, cleaned, brewer's yeast breast is obtained after separation；2. weighing yeast milk 1g as sample, pure water dissolving, 250ml, referred to as sample are settled to；3. after obtaining 50 sample above at random, using pure water as blank, 50 are detected respectively under 600nm wavelength conditions with loading This solution absorbance value A, obtain the absorbance of each sample；Then the dry of 50 samples is contained with national standard method Amount is detected, and obtains the dry matter content of each sample；4. model foundation is carried out, using the absorbance A of each sample of acquisition as abscissa, with each sample of acquisition Dry matter content is ordinate, obtains the standard curve between absorbance A and dry matter content；5. the measure of brewer's yeast milk dry matter content in actual production, beer yeast slurry removal of impurities, cleaning in actual production are taken, point Brewer's yeast breast is obtained from after, yeast milk 1g is weighed as detection sample, pure water dissolving, is settled to 250ml, referred to as detects sample This, using pure water as blank, the solution absorbance value A of the detection sample is measured under 600nm wavelength conditions, is asked on standard curve Obtain the dry matter content of the detection sample, you can obtain the dry matter content of brewer's yeast breast in actual production.
- A kind of 2. method of quick measure brewer's yeast milk dry matter according to claim 1, it is characterised in that：The mark The equation of directrix curve is：Y=22.114X-0.1779；Wherein X-absorbance A, Y-dry matter content；Coefficient of determination R2= 0.9991。
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