CN105128345B - A kind of micropore container and preparation method thereof - Google Patents
A kind of micropore container and preparation method thereof Download PDFInfo
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- CN105128345B CN105128345B CN201510486267.0A CN201510486267A CN105128345B CN 105128345 B CN105128345 B CN 105128345B CN 201510486267 A CN201510486267 A CN 201510486267A CN 105128345 B CN105128345 B CN 105128345B
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Abstract
The invention discloses a kind of micropore container and preparation method thereof, including：Base plate, microporous hollow cylinder, top cover plate and bottom surface film, the top cover plate is arranged on base plate top, the top cover plate bottom is provided with several closure pipes extended respectively in microporous hollow cylinder, the microporous hollow cylinder difference array distribution is on base plate, a servo-actuated buoyant block is respectively arranged with the microporous hollow cylinder, the bottom surface film is arranged on the lower section of microporous hollow cylinder, and the bottom surface film is located at the position of microporous hollow cylinder bottom respectively to the lower section evagination of microporous hollow cylinder.Through the above way, micropore container of the present invention and preparation method thereof, bottom is more frivolous, stickiness is good, firing rate is fast, and heat conduction is more uniform, and the servo-actuated buoyant block of covering is placed in microporous hollow cylinder, reaction solution is advantageously reduced because high temperature or its volatility are lost in, and reduce because of the heat exchange that top cover plate and temperature of charge difference cause.
Description
Technical field
The present invention relates to micropore field of containers, more particularly to a kind of micropore container and preparation method thereof.
Background technology
During PCR, reagent carries out duplication multiplication in each cycle by temperature cycles to original DNA fragment.Often
Individual temperature cycle is generally made up of three steps：（1）Denaturation（~95°C）；（2）Annealing（~50°C）；（3）Extension/extend（~70°C）.
There are two important factors very crucial for PCR detections：One is that the sample being seated in microwell plate can be rapidly achieved its temperature
Degree set point to complete whole test in the short period of time；Two is that, for each temperature set-point, reaction module keeps
The uniformity of all micropore reaction temperatures of microwell array.
Patent of invention（The patent No.：ZL 201210091110.4）Disclose one based on flat micropore container biology or
Chemical reaction method, although its flat micropore container can be heated quickly and uniformly, but due to Structure Designing Problem, it is flat
The process of micropore vessel fabrication is more complicated, and particularly base material thickness is big, and the laminating degree with heater is poor, and heat conduction is relative
It is relatively slow, and internal shortage buoyant block, it is impossible to effectively reduce the loss of reaction solution.
The content of the invention
The present invention solves the technical problem of a kind of micropore container and preparation method thereof is provided, obtaining one kind can be fast
The fast and uniform micropore container heated to reaction solution, and reduce the loss of reaction solution.
In order to solve the above technical problems, one aspect of the present invention is：A kind of micropore container is provided, including：
Base plate, microporous hollow cylinder, top cover plate and bottom surface film, the top cover plate are arranged on base plate top, it is described on
Portion cover plate bottom is provided with several closure pipes extended respectively in microporous hollow cylinder, the microporous hollow cylinder difference array distribution
On base plate, a servo-actuated buoyant block is respectively arranged with the microporous hollow cylinder, the bottom surface film is arranged on micropore
The lower section of hollow cylinder, the bottom surface film is located at the position of microporous hollow cylinder bottom respectively to the lower section evagination of microporous hollow cylinder.
In a preferred embodiment of the present invention, the base plate and microporous hollow cylinder are plastic cement integral structure, institute
It is plastic cement integral structure that closure pipe is stated with top cover plate, and the two ends of the microporous hollow cylinder protrude the upper table of base plate respectively
Face and lower surface.
In a preferred embodiment of the present invention, the outside diameter of the servo-actuated buoyant block is equal to or less than microporous hollow
The internal diameter of cylinder, the bottom of the servo-actuated buoyant block is provided with the arc surface of indent.
In a preferred embodiment of the present invention, the density of the servo-actuated buoyant block is less than water, the servo-actuated buoyant block
Surface is provided with photothermal reflectance material.
In a preferred embodiment of the present invention, the bottom surface film is constituted by thin film or plural layers are compound.
In a preferred embodiment of the present invention, photothermal reflectance layer, the photothermal reflectance are provided with the bottom surface film
Layer is photothermal reflectance film or photothermal reflectance coating.
In a preferred embodiment of the present invention, the bottom surface film is be covered in all microporous hollow cylinder bottoms whole
Film.
In a preferred embodiment of the present invention, the bottom surface film includes several corresponding with microporous hollow cylinder bottom respectively
Circular sub- film, the bottom margin of the edge of the circular sub- film respectively with microporous hollow cylinder is connected.
In order to solve the above technical problems, one aspect of the present invention is：A kind of making of micropore container is provided
Method, comprises the following steps：
Shaped by plastic mould, obtain the closure pipe and top cover plate of integral structure；
Shaped by plastic mould, obtain the base plate and microporous hollow cylinder of integral structure；
Shaped by plastic mould, obtain servo-actuated buoyant block, one layer of photothermal reflectance material is coated with the surface of servo-actuated buoyant block
Material；
The compound composition bottom surface film of selection plural layers, the material melting point of the film of top layer is slightly less than or waits in plural layers
With the fusing point of microporous hollow cylinder material, fusing point of the fusing point of the film of bottom higher than microporous hollow cylinder material in plural layers；
Bottom surface film is entirely sealed by melt process the bottom or bottom surface is thin for being fixed on all microporous hollows cylinders
Film is cut into and circular sub- film difference hot-melting sealed is existed again with after the circular sub- film of size identical of single microporous hollow cylinder bottom
The bottom of microporous hollow cylinder；
To reaction solution is injected in microporous hollow cylinder, servo-actuated buoyant block is individually positioned in microporous hollow cylinder and swims in reaction
On liquid, then top cover plate is arranged on base plate so that closure pipe is blocked to microporous hollow cylinder respectively.
In a preferred embodiment of the present invention, the melt process is：High temperature flat board is preheated, surface temperature is allowed to
Spend the melting temperature of the film of top layer in a little higher than plural layers, but the film less than bottom melting temperature, followed by height
Bottom surface film is pressed to warm flat board the bottom surface of microporous hollow cylinder, of short duration holding pressure 0.1 ~ 5 second so that the film melts of top layer
And combined with the bottom surface of microporous hollow cylinder, then high temperature flat board is separated, and room temperature is down in the bottom surface of bottom surface film and microporous hollow cylinder
After realize and be tightly connected, microporous hollow cylinder drop back to after room temperature due to sandwich construction bottom surface film shrinkage factor difference so that bottom
Face film is to protrusion outside microporous hollow cylinder.
The beneficial effects of the invention are as follows：A kind of micropore container that the present invention is pointed out and preparation method thereof, using bottom surface film
Slight evagination, be conducive to microporous hollow cylinder bottom and flat temp controlled meter face contact when, can more be adjacent to the surface, it is good to reach
Good thermo-contact, heat conduction is more uniform, and more frivolous, thermal conductivity is good, and firing rate is fast, and covering is placed in microporous hollow cylinder
Servo-actuated buoyant block, advantageously reduces reaction solution because high temperature or its volatility are lost in.
Brief description of the drawings
Technical scheme in order to illustrate more clearly the embodiments of the present invention, below will be to that will make needed for embodiment description
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention, for
For those of ordinary skill in the art, on the premise of not paying creative work, other can also be obtained according to these accompanying drawings
Accompanying drawing, wherein：
Fig. 1 is a kind of structural representation of micropore container of the invention an and preparation method thereof preferred embodiment；
Fig. 2 is the explosive view of Fig. 1；
Fig. 3 is a kind of structural representation of micropore container of the invention and preparation method thereof another preferred embodiment.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common
All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
Fig. 1 to Fig. 3 is referred to, the embodiment of the present invention includes：
A kind of micropore container, including：Base plate 1, microporous hollow cylinder 2, top cover plate 3 and bottom surface film 6, the top
Cover plate 3 is arranged on the top of base plate 1, and the bottom of top cover plate 3 is provided with several extending respectively in microporous hollow cylinder 2
Closure pipe 4, the microporous hollow cylinder 2 distinguishes array distribution on base plate 1, is respectively arranged with the microporous hollow cylinder 2
One servo-actuated buoyant block 5, the bottom surface film 6 is arranged on the lower section of microporous hollow cylinder 2, and it is empty that the bottom surface film 6 is located at micropore
The position of 2 bottoms of heart cylinder is respectively to the lower section evagination of microporous hollow cylinder 2.The bottom surface film 6 of evagination is easier and adds in heating
The surface of thermal fits, and increases contact area, improves firing rate and uniformity.
The base plate 1 is plastic cement integral structure with microporous hollow cylinder 2, and the closure pipe 4 is modeling with top cover plate 3
Glue integral structure, the two ends of the microporous hollow cylinder 2 protrude the upper and lower surface of base plate 1 respectively, it is to avoid micropore
Reaction solution in hollow cylinder 2 is splashed on base plate 1.
The outside diameter of the servo-actuated buoyant block 5 is equal to or less than the internal diameter of microporous hollow cylinder 2, the servo-actuated buoyant block
5 bottom is provided with the arc surface of indent, and reaction solution is in heating, and liquid is readily volatilized, and servo-actuated buoyant block 5 is arranged on reaction solution
On, the arc surface of its indent effectively reduces the volatilization of reaction solution.
The density of the servo-actuated buoyant block 5 is less than water, and the surface of the servo-actuated buoyant block 5 is provided with photothermal reflectance material,
The light and heat of the lower surface of servo-actuated buoyant block 5 can be reflexed to inside reaction solution, improve the efficiency of heating surface of reaction solution, reduce heat
Loss.
The bottom surface film 6 is constituted by thin film or plural layers are compound.During plural layers, every layer of fusing point is not
Together, and lower floor film melting points higher than its upper strata film melting points.
Photothermal reflectance layer is provided with the bottom surface film 6, the photothermal reflectance layer is that photothermal reflectance film or photo-thermal are anti-
Penetrate coating.
The bottom surface film 6 is whole film for being covered in all 2 bottoms of microporous hollow cylinder, such as Fig. 3.Whole film heat absorption
With the speed of heat conduction faster, but the pattern after the heat distortion of bottom surface film 6 is coarse, not as single circular sub- film comes beautiful
See, or the bottom surface film 6 include it is several respectively with the corresponding circular sub- films in microporous hollow 2 bottoms of cylinder, such as Fig. 1 and Fig. 2,
Bottom margin of the edge of the circular sub- film respectively with microporous hollow cylinder 2 is connected.
A kind of preparation method of micropore container, comprises the following steps：
Shaped by plastic mould, the closure pipe 4 and top cover plate of integral structure are obtained using polypropylene material injection
3；
Shaped by plastic mould, the base plate 1 for obtaining integral structure using polypropylene material injection is empty with micropore
Heart cylinder 2；
Shaped by plastic mould, servo-actuated buoyant block 5 is obtained using polypropylene material, servo-actuated buoyant block 5 uses polypropylene
Material is made, and density is less than reaction solution so that servo-actuated buoyant block 5 is able to swim in the surface of reaction solution, in servo-actuated buoyant block 5
Surface is coated with one layer of photothermal reflectance material to improve the efficiency of heating surface；
The compound composition bottom surface film 6 of selection plural layers, in plural layers the material melting point of the film of top layer be slightly less than or
The fusing point of 2 materials of equivalent microporous hollow cylinder, the fusing point of the film of bottom is molten higher than microporous hollow 2 materials of cylinder in plural layers
Point；
By melt process, bottom surface film 6, entirely sealing is fixed on the bottom of all microporous hollow cylinders 2 or bottom surface
Film is cut into distinguishes hot-melting sealed circular sub- film again with after the bottom circular sub- film of size identical of single microporous hollow cylinder 2
In the bottom of microporous hollow cylinder 2；
To reaction solution is injected in microporous hollow cylinder 2, servo-actuated buoyant block 5 is individually positioned in microporous hollow cylinder 2 and is swum in
On reaction solution, then top cover plate 3 is arranged on base plate 1 so that closure pipe 4 is sealed to microporous hollow cylinder 2 respectively
It is stifled.
The melt process is：High temperature flat board is preheated, top layer in a little higher than plural layers of surface temperature is allowed to
The melting temperature of film, but the film less than bottom melting temperature, bottom surface film is pressed into micropore followed by high temperature flat board
The bottom surface of hollow cylinder 2, of short duration holding pressure 0.1 ~ 5 second so that the film melts of top layer and with microporous hollow cylinder 2 bottom surface tie
Close, then high temperature flat board is separated, the bottom surface of bottom surface film 6 and microporous hollow cylinder 2 is down to after room temperature to realize and is tightly connected, micropore
Hollow cylinder 2 drop back to after room temperature due to sandwich construction bottom surface film 6 shrinkage factor difference so that bottom surface film 6 is to microporous hollow cylinder
2 outer protrusions.
In sum, a kind of micropore container that the present invention is pointed out and preparation method thereof, when the micropore container for obtaining is heated
Heat-transfer rate block, more efficiently, and heating uniformity is good for heating, is conducive to improving the accuracy of experimental data.
Embodiments of the invention are the foregoing is only, the scope of the claims of the invention is not thereby limited, it is every to utilize this hair
Equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, is included within the scope of the present invention.
Claims (2)
1. a kind of preparation method of micropore container, for the production of micropore container, the micropore container includes：It is base plate, micro-
Hole hollow cylinder, top cover plate and bottom surface film, the top cover plate are arranged on base plate top, and the top cover plate bottom sets
It is equipped with several closure pipes extended respectively in microporous hollow cylinder, it is characterised in that the microporous hollow cylinder difference array distribution
On base plate, a servo-actuated buoyant block is respectively arranged with the microporous hollow cylinder, the bottom surface film is arranged on micropore
The lower section of hollow cylinder, the bottom surface film is located at the position of microporous hollow cylinder bottom respectively to the lower section evagination of microporous hollow cylinder,
The base plate is plastic cement integral structure with microporous hollow cylinder, and the closure pipe is that plastic cement is integrated with top cover plate to be tied
Structure, the two ends of microporous hollow cylinder protrude the upper and lower surface of base plate respectively, the servo-actuated buoyant block it is cylindrical
Diameter is equal to or less than the internal diameter of microporous hollow cylinder, and the bottom of the servo-actuated buoyant block is provided with the arc surface of indent, described
The density of servo-actuated buoyant block is less than water, and the surface of the servo-actuated buoyant block is provided with photothermal reflectance material, the bottom surface film by
Thin film or the compound composition of plural layers, are provided with photothermal reflectance layer on the bottom surface film, the photothermal reflectance layer is
Photothermal reflectance film or photothermal reflectance coating, the bottom surface film are whole film for being covered in all microporous hollow cylinder bottoms,
The bottom surface film includes several circular sub- films corresponding with microporous hollow cylinder bottom respectively, the edge of the circular sub- film
The bottom margin with microporous hollow cylinder is connected respectively, it is characterised in that comprise the following steps：
Shaped by plastic mould, obtain the closure pipe and top cover plate of integral structure；
Shaped by plastic mould, obtain the base plate and microporous hollow cylinder of integral structure；
Shaped by plastic mould, obtain servo-actuated buoyant block, one layer of photothermal reflectance material is coated with the surface of servo-actuated buoyant block；
The compound composition bottom surface film of selection plural layers, the material melting point of the film of top layer is slightly less than or equivalent micro- in plural layers
The fusing point of hole hollow cylinder material, fusing point of the fusing point of the film of bottom higher than microporous hollow cylinder material in plural layers；
Bottom surface film is entirely sealed by melt process is fixed on the bottom of all microporous hollows cylinders or bottom surface film is cut
Into with the single microporous hollow circular sub- film of cylinder bottom size identical after circular sub- film is distinguished hot-melting sealed in micropore again
The bottom of hollow cylinder；
To reaction solution is injected in microporous hollow cylinder, servo-actuated buoyant block is individually positioned in microporous hollow cylinder and swims in reaction solution
On, then top cover plate is arranged on base plate so that closure pipe is blocked to microporous hollow cylinder respectively.
2. the preparation method of micropore container according to claim 1, it is characterised in that the melt process is：To high temperature
Flat board is preheated, and is allowed to the melting temperature of the film of top layer in a little higher than plural layers of surface temperature, but thin less than bottom
The melting temperature of film, bottom surface film is pressed to the bottom surface of microporous hollow cylinder, of short duration holding pressure 0.1 ~ 5 followed by high temperature flat board
Second so that the film melts of top layer and is combined with the bottom surface of microporous hollow cylinder, then high temperature flat board separation, bottom surface film and micro-
The bottom surface of hole hollow cylinder is down to after room temperature to realize and is tightly connected, and microporous hollow cylinder is dropped back to after room temperature due to the bottom surface of sandwich construction
The shrinkage factor difference of film causes bottom surface film to protrusion outside microporous hollow cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510486267.0A CN105128345B (en) | 2015-08-10 | 2015-08-10 | A kind of micropore container and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510486267.0A CN105128345B (en) | 2015-08-10 | 2015-08-10 | A kind of micropore container and preparation method thereof |
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| CN105128345A CN105128345A (en) | 2015-12-09 |
| CN105128345B true CN105128345B (en) | 2017-06-09 |
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| CN110218628B (en) * | 2019-06-19 | 2021-01-29 | 中国科学院半导体研究所 | Digital PCR chip and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03297378A (en) * | 1990-04-13 | 1991-12-27 | Seiko Instr Inc | Automatic reactor |
| US6703236B2 (en) * | 1990-11-29 | 2004-03-09 | Applera Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
| US5459300A (en) * | 1993-03-03 | 1995-10-17 | Kasman; David H. | Microplate heater for providing uniform heating regardless of the geometry of the microplates |
| DE29623597U1 (en) * | 1996-11-08 | 1999-01-07 | Eppendorf Geraetebau Netheler | Temperature control block with temperature control devices |
| US8968684B2 (en) * | 2011-04-28 | 2015-03-03 | Bin Lian | Microplates, reaction modules and detection systems |
| CN204874497U (en) * | 2015-08-10 | 2015-12-16 | 苏州晋翌生物医学仪器有限公司 | Micropore container |
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Effective date of registration: 20210508 Address after: 310000 Room 302, building 5, 1818-2, Wenyi West Road, Yuhang street, Yuhang District, Hangzhou City, Zhejiang Province Patentee after: Zhejiang Jushi Information Technology Co.,Ltd. Address before: 215500 Room 203, science and Technology Park, Changshu Economic and Technological Development Zone, Suzhou City, Jiangsu Province Patentee before: Suzhou Jinyi Biomedical Instrument Co.,Ltd. |