CN105125599B - A kind of preparation method of the tribulus terrestris extraction containing total saponins of tribulus - Google Patents
A kind of preparation method of the tribulus terrestris extraction containing total saponins of tribulus Download PDFInfo
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- CN105125599B CN105125599B CN201510498286.5A CN201510498286A CN105125599B CN 105125599 B CN105125599 B CN 105125599B CN 201510498286 A CN201510498286 A CN 201510498286A CN 105125599 B CN105125599 B CN 105125599B
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- tribulus
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- ethyl alcohol
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- MUKYLHIZBOASDM-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]acetic acid 2,3,4,5,6-pentahydroxyhexanoic acid Chemical compound NC(=N)N(C)CC(O)=O.OCC(O)C(O)C(O)C(O)C(O)=O MUKYLHIZBOASDM-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 229930182490 saponin Natural products 0.000 title claims abstract description 24
- 150000007949 saponins Chemical class 0.000 title claims abstract description 24
- 235000017709 saponins Nutrition 0.000 title claims abstract description 24
- 241000819233 Tribulus <sea snail> Species 0.000 title claims abstract description 23
- 238000000605 extraction Methods 0.000 title claims abstract description 23
- 229940047183 tribulus Drugs 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 241001521901 Tribulus lanuginosus Species 0.000 title claims abstract 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000004952 Polyamide Substances 0.000 claims abstract description 16
- 229920002647 polyamide Polymers 0.000 claims abstract description 16
- 235000021419 vinegar Nutrition 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 238000009835 boiling Methods 0.000 claims abstract description 13
- 239000000052 vinegar Substances 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 49
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 25
- 239000003480 eluent Substances 0.000 claims description 25
- 239000000741 silica gel Substances 0.000 claims description 25
- 229910002027 silica gel Inorganic materials 0.000 claims description 25
- 229960001866 silicon dioxide Drugs 0.000 claims description 25
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 21
- 235000019441 ethanol Nutrition 0.000 claims description 20
- 239000000047 product Substances 0.000 claims description 13
- 239000011347 resin Substances 0.000 claims description 11
- 229920005989 resin Polymers 0.000 claims description 11
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 claims description 10
- 238000010828 elution Methods 0.000 claims description 10
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 7
- 239000012043 crude product Substances 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 238000004587 chromatography analysis Methods 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000001764 infiltration Methods 0.000 claims description 5
- 230000008595 infiltration Effects 0.000 claims description 5
- 239000012044 organic layer Substances 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 230000006837 decompression Effects 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims 2
- 238000004440 column chromatography Methods 0.000 abstract description 2
- 238000003810 ethyl acetate extraction Methods 0.000 abstract description 2
- 238000010898 silica gel chromatography Methods 0.000 abstract 1
- 241001533104 Tribulus terrestris Species 0.000 description 28
- 239000000243 solution Substances 0.000 description 23
- 238000010025 steaming Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 238000001816 cooling Methods 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000001925 catabolic effect Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 150000005856 steroid saponins Chemical class 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 102000009151 Luteinizing Hormone Human genes 0.000 description 2
- 108010073521 Luteinizing Hormone Proteins 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 150000002338 glycosides Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000003808 methanol extraction Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 229960003604 testosterone Drugs 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- DVGGLGXQSFURLP-VWMSDXGPSA-N tribuloside Chemical compound C([C@@H]1[C@H]([C@@H]([C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=CC(O)=CC=2)=O)O1)O)O)OC(=O)\C=C\C1=CC=C(O)C=C1 DVGGLGXQSFURLP-VWMSDXGPSA-N 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 240000006122 Chenopodium album Species 0.000 description 1
- 235000009344 Chenopodium album Nutrition 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 229920006122 polyamide resin Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
Abstract
Description
Total saponins of tribulus(mg/100ml) | |
Control group | 13.22 |
Water steaming group 1 | 15.17 |
Vinegar water steaming group 1 | 22.07 |
Saline solution steaming group 1 | 14.35 |
Water steaming group 2 | 18.54 |
Vinegar water steaming group 2 | 30.89 |
Saline solution steaming group 2 | 20.21 |
Vacuum drying | Vacuum freezedrying | Spray drying | Micro-wave vacuum | |
Total saponins of tribulus content | 95.17 | 96.58 | 95.14 | 98.01 |
Moisture | 1.82 | 1.37 | 2.21 | 0.79 |
Drying time | 1h | 1h | 1h | 1h |
Dissolvent residual | Nothing | Nothing | 1.5 | Nothing |
Claims (5)
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CN201510498286.5A CN105125599B (en) | 2015-08-14 | 2015-08-14 | A kind of preparation method of the tribulus terrestris extraction containing total saponins of tribulus |
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CN201510498286.5A CN105125599B (en) | 2015-08-14 | 2015-08-14 | A kind of preparation method of the tribulus terrestris extraction containing total saponins of tribulus |
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CN105125599A CN105125599A (en) | 2015-12-09 |
CN105125599B true CN105125599B (en) | 2018-07-13 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106317160A (en) * | 2016-07-26 | 2017-01-11 | 西安岳达生物科技股份有限公司 | Tribulus terrestris saponin K extracting and separating method |
CN110227047B (en) * | 2019-06-26 | 2022-05-13 | 广州三广生物科技有限公司 | Skin care composition with effect of removing red blood streak and preparation method thereof |
CN111494440A (en) * | 2020-04-24 | 2020-08-07 | 武汉轻工大学 | Extraction, purification and preparation technology of blood sugar reducing component saponin in caltrop, caltrop saponin extract and application |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102178706A (en) * | 2011-01-24 | 2011-09-14 | 南京泽朗医药科技有限公司 | Method for extracting tribuloside |
CN103040880A (en) * | 2012-12-13 | 2013-04-17 | 大兴安岭林格贝有机食品有限责任公司 | Separation and purification method of tribulus terrestris saponin |
DE112012003484T5 (en) * | 2011-08-23 | 2014-05-15 | Mustafa Eraslan | Tribulus terrestris, Avena sativa and Panax ginseng extract combination |
-
2015
- 2015-08-14 CN CN201510498286.5A patent/CN105125599B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102178706A (en) * | 2011-01-24 | 2011-09-14 | 南京泽朗医药科技有限公司 | Method for extracting tribuloside |
DE112012003484T5 (en) * | 2011-08-23 | 2014-05-15 | Mustafa Eraslan | Tribulus terrestris, Avena sativa and Panax ginseng extract combination |
CN103040880A (en) * | 2012-12-13 | 2013-04-17 | 大兴安岭林格贝有机食品有限责任公司 | Separation and purification method of tribulus terrestris saponin |
Non-Patent Citations (1)
Title |
---|
正交试验法优选刺蒺藜皂苷的提取工艺;王霄?等;《西北药学杂志》;20080115;第23卷(第01期);24-26 * |
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