CN105125594B - A kind of antrodia Anethol Trithione composition that treating liver cancer and preparation method - Google Patents
A kind of antrodia Anethol Trithione composition that treating liver cancer and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of antrodia Anethol Trithione composition for treating liver cancer and preparation methods.The component and part of the composition includes 20~25 parts of antrodia, 3~5 parts of Anethol Trithione, 15~20 parts of diluent, 0.5~1 part of surfactant, 15~20 parts of disintegrating agent, and 0.1~0.5 part of lubricant.The preparation method of the composition includes stock, the extraction of antrodia effective component, wet granulation, whole grain, tabletting.The present invention combines two kinds of drug antrodias, Anethol Trithiones, reduces the type and pharmaceutic adjuvant dosage of bulk pharmaceutical chemicals to greatest extent.On the basis of antrodia inhibits liver cancer, Anethol Trithione can promote the recovery of liver cell, and the two synergistic effect is obvious, can effectively treat liver cancer.Cancer patient will not because of chemotherapy side effect and the defects of cause autoimmunity to reduce.Composition preparation process of the invention is simple, easy to industrialized production, convenient to take, improves the doctor of patient from property, thus therapeutic effect can be more preferable.
Description
Technical field
It is especially a kind of antrodia Anethol Trithione group for treating liver cancer the invention belongs to treat the field of medicinal compositions of liver cancer
Close object and preparation method.
Background technique
Antrodia (Antrodia camphorata) is the distinctive rare medicinal fungi in China, and single-minded parasitizes cinnamomum kanehirai
On, the chemical component of antrodia is extremely complex, and modern separation technology confirms, antrodia contains anti-cancer, anticancer, the physiology for improving immunity
Active material, main component are polysaccharide body and triterpene compound.Triterpene compound is that current antrodia fructification discovery is most
Terpene chemical component, be generally acknowledged to the main source of bitter taste composition in antrodia extract.Wherein up to more than 10 kinds of new discoveries
Triterpenes is exclusive for antrodia, and experiment confirms: these triterpene compounds can inhibit the proliferation of liver cancer cells, poisoning mouse leukemia
(P-388 murine leukemia) cell activity, the effect of anti-parasympathetic nerve, antiserotonin activity, activation nerve cell are raw
The functions such as long ability；Prior anti-tumor activity, which is play, than polysaccharide body adjusts role.Hypertensive patient is often because of blood pressure
Height, leads to rupture of blood vessel in brain and apoplexy, triterpene compound can effectively inhibit Angiotensin Converting Enzyme
The activity of (ACE Angiotensin-Converting), and then reduce blood pressure.Application No. is 200510044806.1 patents to disclose one
The antrodia capsule and preparation method thereof of kind anti-liver cancer and anti-；Please number for 201310088358.X a patent disclose antrodia fructification with
The composition of ganoderma spove powder and its application in immunological regulation, antrodia fructification and lucidum spore powder are all somewhat expensive
Drug, obtained composition price is somewhat expensive, is not that the family commonly taken in can consume purchase；Application No. is
201010502077.0 patent disclose Antrodia camphorata anti-cancer active matter, preparation method and its usage；In addition there are also other specially
Benefit is all the extracting method for studying Antrodia camphorata anti-cancer active matter, for the fewer of antrodia composition research, especially to such as
The composition what improves antrodia drug effect is studied currently without people.
Summary of the invention
It is fewer the purpose of the present invention is being studied for current antrodia composition, especially to how improving antrodia drug effect
The defect that composition is studied currently without people proposes that one kind can be improved antrodia drug effect, and two kinds of drugs are organically combined one
It rises, the antrodia Anethol Trithione composition and preparation method of the high treatment liver cancer of drug effective rate of utilization.
The object of the invention is realized by the following technical solutions:
A kind of antrodia Anethol Trithione composition for treating liver cancer, the component and part of the composition include antrodia 20~
25 parts, 3~5 parts of Anethol Trithione, 15~20 parts of diluent, 0.5~1 part of surfactant, 15~20 parts of disintegrating agent, lubricant 0.1
~0.5 part.
Further, a kind of antrodia Anethol Trithione composition for treating liver cancer, the component and parts by weight of the composition
Number include 22 parts of antrodia, 4 parts of Anethol Trithione, 18 parts of diluent, 0.7 part of surfactant, 17 parts of disintegrating agent, 0.3 part of lubricant.
Further, a kind of antrodia Anethol Trithione composition for treating liver cancer, diluent are as follows: starch, Icing Sugar, paste
At least one of essence, amylum pregelatinisatum, microcrystalline cellulose.
Further, a kind of antrodia Anethol Trithione composition for treating liver cancer, surfactant are as follows: fatty acid sorb
At least one of smooth, polysorbate, polyoxyethylene fatty acid ester, Pluronic F68.
Further, a kind of antrodia Anethol Trithione composition for treating liver cancer, disintegrating agent are as follows: dried starch, carboxymethyl
Starch receives, at least one of low-substituted hydroxypropyl cellulose, crosslinked polyvinylpyrrolidone, croscarmellose sodium.
Further, a kind of antrodia Anethol Trithione composition for treating liver cancer, lubricant are as follows: magnesium stearate, micro mist
At least one of silica gel, talcum powder.
The present invention also provides a kind of preparation method of antrodia Anethol Trithione composition for treating liver cancer, specific steps
Include:
1) it stocks up, according to a kind of component and part stock for the antrodia Anethol Trithione composition for treating liver cancer；
2) extraction of antrodia effective ingredient: crushed 200 meshes for antrodia, and 4 times of bodies of antrodia are added into ultrasonic extraction tank
Long-pending alcohol, alcohol quality concentration are 95%, smashed antrodia are added in ultrasonic extraction tank, ultrasonic 6h takes supernatant mark
It is denoted as a solution；Again into ultrasonic extraction tank be added 2 times of volumes of antrodia alcohol, alcohol quality concentration be 95%, ultrasonic 6h,
It takes supernatant labeled as b solution, merges solution a, b and obtain solution A, by solution A pressure is -0.06~-0.08Mpa, temperature is
/ 5th that solution A volume is concentrated under the conditions of 40~45 DEG C obtain concentrate B；
3) wet granulation: by the other of concentrate B obtained by step 2) and a kind of antrodia Anethol Trithione composition for treating liver cancer
Component wet granulation together, the drying equipment of wet granulation are fluidized bed, set the drying temperature of fluidized bed as 55~60 DEG C, when
Between be 15~30min, obtain particle C；
4) whole grain: particle C obtained by step 3) be crushed into 20~40 meshes and obtain particle D；
5) tabletting: particle D obtained by step 4) is subjected to tabletting by tablet press machine, slice weight is 0.5~0.8g.
In order to more effectively treat liver cancer, the present invention passes through a large amount of screening tests, by the effective component of Chinese medicine antrodia with Western medicine
Anethol Trithione combines.Antrodia effective component triterpene compound can inhibit the proliferation of liver cancer cells, poisoning mouse
The functions such as blood cell activity, the effect of anti-parasympathetic nerve, antiserotonin activity, activation nerve growth ability；Compare polysaccharide
Body plays prior anti-tumor activity and adjusts role；Antrodia polysaccharide has antifatigue, antitumor, raising immunity, drop blood
The multiple biological activities such as sugar, liver protection, anti-oxidant.The effect of Anethol Trithione are as follows: 1) promote bile discharge, make cholic acid, BILE PIGMENTS and
The secretory volume of the solid components such as cholesterol dramatically increases, especially the secretion of increase BILE PIGMENTS；2) liver can also be remarkably reinforced in this medicine
Glutathione level significantly increases glutamylcysteine ??synthase, glutathione reductase isoreactivity, to significantly increase liver
Cell viability increases bile secretion.The symptoms such as the hepatohemia of hepatitis lesion can be eliminated, promotes liver cell activation, is conducive to liver
Functional rehabilitation is normal；3) this medicine has ptyalize to promote digestion, can promote salivary secretion, subtract to saliva caused by antipsychotics
It is few especially effective；Gas in gastrointestinal motility and intestinal tube can be promoted to be discharged, abdominal distension, constipation, halitosis, nausea, abdomen can be eliminated rapidly
The indigestion symptoms such as pain；4) this medicine can promote internal alcohols material quick generation there are also cholesterol effect and detoxication is decomposed
It thanks and eliminates, reduce blood middle cholesterol content and prevent it calm or be attached to blood vessel；Alcohol, drug, food etc. are drawn
The poisoning risen has good detoxication and anti-allergic effects；5) this medicine can also promote the production and excretion of urea, have obvious
Diuresis.While guaranteeing to be easy tabletting, antrodia effective component and Anethol Trithione can be more for the selection of pharmaceutic adjuvant of the present invention
Good dissolution and absorption.
The selection of present invention process: the extraction process simple and stable of antrodia effective component, it is reproducible, by effective component
Extraction cost is minimized while utmostly extraction.The selection of wet granulation technology guarantees in granulating process operation
It not will lead to the loss of activity of antrodia effective component and Anethol Trithione, while being conducive to the operation of tableting process.
Beneficial effects of the present invention:
The present invention combines two kinds of drug antrodias, Anethol Trithiones, reduces bulk pharmaceutical chemicals to greatest extent
Type and pharmaceutic adjuvant dosage.Antrodia triterpene has the effects of increment for inhibiting liver cancer cells, and antrodia polysaccharide has antitumor, raising to exempt from
The effects of epidemic disease power, liver protection；Anethol Trithione can promote bile discharge, enhancing Activity of hepatocytes to be conducive to, and liver function recovery is normal etc. to be made
With.On the basis of antrodia inhibits liver cancer, Anethol Trithione can promote the recovery of liver cell, and the two synergistic effect is obvious, can be more effective
Treatment liver cancer.Hepatocarcinoma patient do not need carry out chemotherapy, therefore will not because of chemotherapy side effect and cause autoimmunity to drop
Low defect.Composition preparation process of the invention is simple, easy to industrialized production, convenient to take, improve the doctor of patient from
Property, thus therapeutic effect can be more preferable.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies specific material proportion, process conditions and its result described in example and is merely to illustrate the present invention without that should will not limit
The present invention described in detail in claims.
Embodiment 1
A kind of antrodia Anethol Trithione composition for treating liver cancer, the component and part of the composition include 22 parts of antrodia,
4 parts of Anethol Trithione, 18 parts of microcrystalline cellulose, 0.7 part of polysorbate, carboxymethyl starch receive 17 parts, 0.3 part of superfine silica gel powder.
A kind of preparation method of antrodia Anethol Trithione composition that treating liver cancer, specific steps include:
1) it stocks up, stocks up according to component and part described in a kind of antrodia Anethol Trithione composition for treating liver cancer；
2) extraction of antrodia effective ingredient triterpene: crushed 200 meshes for antrodia, and antrodia 4 is added into ultrasonic extraction tank
The alcohol of times volume, alcohol quality concentration are 95%, smashed antrodia are added in ultrasonic extraction tank, ultrasonic 6h takes supernatant
Liquid is labeled as a solution；The alcohol of 2 times of volumes of antrodia is added into ultrasonic extraction tank again, alcohol quality concentration is 95%, ultrasound
6h takes supernatant labeled as b solution, merges solution a, b and obtains solution A, in pressure is -0.06~-0.08Mpa, temperature by solution A
Degree for be concentrated under the conditions of 40~45 DEG C solution A volume 1/5th concentrate B；
3) wet granulation: by the other of concentrate B obtained by step 2) and a kind of antrodia Anethol Trithione composition for treating liver cancer
Component wet granulation together, the drying equipment of wet granulation are fluidized bed, set the drying temperature of fluidized bed as 55~60 DEG C, when
Between be 15min, obtain particle C；
4) whole grain: particle C obtained by step 3) be crushed into 20 meshes and obtain particle D；
5) particle D obtained by step 4) tabletting: is subjected to tabletting, slice weight 0.5g by tablet press machine.
A kind of application method of antrodia Anethol Trithione composition for treating liver cancer of the invention: daily 2~3 times, 2 tablets once,
Or it follows the doctor's advice.
Storage: shading is sealed.
Embodiment 2
A kind of antrodia Anethol Trithione composition for treating liver cancer, the component and part of the composition include 20 parts of antrodia,
3 parts of Anethol Trithione, 15 parts of amylum pregelatinisatum, 0.5 part of fatty acid sorbitan, 15 parts of low-substituted hydroxypropyl cellulose, talcum powder 0.1
Part.
A kind of preparation method of antrodia Anethol Trithione composition that treating liver cancer, specific steps include:
1) it stocks up, stocks up according to component and part described in a kind of antrodia Anethol Trithione composition for treating liver cancer；
2) extraction of antrodia effective ingredient triterpene: crushed 200 meshes for antrodia, and antrodia 4 is added into ultrasonic extraction tank
The alcohol of times volume, alcohol quality concentration are 95%, smashed antrodia are added in ultrasonic extraction tank, ultrasonic 6h takes supernatant
Liquid is labeled as a solution；The alcohol of 2 times of volumes of antrodia is added into ultrasonic extraction tank again, alcohol quality concentration is 95%, ultrasound
6h takes supernatant labeled as b solution, merges solution a, b and obtains solution A, in pressure is -0.06~-0.08Mpa, temperature by solution A
Degree for be concentrated under the conditions of 40~45 DEG C solution A volume 1/5th concentrate B；
3) wet granulation: by the other of concentrate B obtained by step 2) and a kind of antrodia Anethol Trithione composition for treating liver cancer
Component wet granulation together, the drying equipment of wet granulation are fluidized bed, set the drying temperature of fluidized bed as 55~60 DEG C, when
Between be 20min, obtain particle C；
4) whole grain: particle C obtained by step 3) be crushed into 30 meshes and obtain particle D；
5) particle D obtained by step 4) tabletting: is subjected to tabletting, slice weight 0.8g by tablet press machine.
A kind of application method of antrodia Anethol Trithione composition for treating liver cancer of the invention: daily 2~3 times, 1 tablet once,
Or it follows the doctor's advice.
Storage: shading is sealed.
Embodiment 3
A kind of antrodia Anethol Trithione composition for treating liver cancer, the component and part of the composition include 25 parts of antrodia,
5 parts of Anethol Trithione, 20 parts of Icing Sugar, 1 part of polyoxyethylene fatty acid ester, 20 parts of crosslinked polyvinylpyrrolidone, 0.5 part of magnesium stearate.
A kind of preparation method of antrodia Anethol Trithione composition that treating liver cancer, specific steps include:
1) it stocks up, stocks up according to component and part described in a kind of antrodia Anethol Trithione composition for treating liver cancer；
2) extraction of antrodia effective ingredient triterpene: crushed 200 meshes for antrodia, and antrodia 4 is added into ultrasonic extraction tank
The alcohol of times volume, alcohol quality concentration are 95%, smashed antrodia are added in ultrasonic extraction tank, ultrasonic 6h takes supernatant
Liquid is labeled as a solution；The alcohol of 2 times of volumes of antrodia is added into ultrasonic extraction tank again, alcohol quality concentration is 95%, ultrasound
6h takes supernatant labeled as b solution, merges solution a, b and obtains solution A, in pressure is -0.06~-0.08Mpa, temperature by solution A
Degree for be concentrated under the conditions of 40~45 DEG C solution A volume 1/5th concentrate B；
3) wet granulation: by the other of concentrate B obtained by step 2) and a kind of antrodia Anethol Trithione composition for treating liver cancer
Component wet granulation together, the drying equipment of wet granulation are fluidized bed, set the drying temperature of fluidized bed as 55~60 DEG C, when
Between be 30min, obtain particle C；
4) whole grain: particle C obtained by step 3) be crushed into 40 meshes and obtain particle D；
5) particle D obtained by step 4) tabletting: is subjected to tabletting, slice weight 0.6g by tablet press machine.
A kind of application method of antrodia Anethol Trithione composition for treating liver cancer of the invention: daily 2~3 times, 2 tablets once,
Or it follows the doctor's advice.
Storage: shading is sealed.
Embodiment 4
A kind of antrodia Anethol Trithione composition for treating liver cancer, the component and part of the composition include 24 parts of antrodia,
3.5 parts of Anethol Trithione, 17 parts of dextrin, 0.8 part of Pluronic F68,16 parts of croscarmellose sodium, it is stearic
0.4 part of sour magnesium.
A kind of preparation method of antrodia Anethol Trithione composition that treating liver cancer, specific steps include:
1) it stocks up, stocks up according to component and part described in a kind of antrodia Anethol Trithione composition for treating liver cancer；
2) extraction of antrodia effective ingredient triterpene: crushed 200 meshes for antrodia, and antrodia 4 is added into ultrasonic extraction tank
The alcohol of times volume, alcohol quality concentration are 95%, smashed antrodia are added in ultrasonic extraction tank, ultrasonic 6h takes supernatant
Liquid is labeled as a solution；The alcohol of 2 times of volumes of antrodia is added into ultrasonic extraction tank again, alcohol quality concentration is 95%, ultrasound
6h takes supernatant labeled as b solution, merges solution a, b and obtains solution A, in pressure is -0.06~-0.08Mpa, temperature by solution A
/ 5th that solution A volume is concentrated under the conditions of being 40~45 DEG C obtain concentrate B；
3) wet granulation: by the other of concentrate B obtained by step 2) and a kind of antrodia Anethol Trithione composition for treating liver cancer
Component wet granulation together, the drying equipment of wet granulation are fluidized bed, set the drying temperature of fluidized bed as 55~60 DEG C, when
Between be 25min, obtain particle C；
4) whole grain: particle C obtained by step 3) be crushed into 30 meshes and obtain particle D；
5) particle D obtained by step 4) tabletting: is subjected to tabletting, slice weight 0.7g by tablet press machine.
A kind of application method of antrodia Anethol Trithione composition for treating liver cancer of the invention: daily 2~3 times, 1 tablet once,
Or it follows the doctor's advice.
Storage: shading is sealed.
Slaking test inspection of the invention:
Check method inspection is examined according to " Chinese Pharmacopoeia " 2010 editions (two) annex X A disintegration time limiteds.
Instrument: lift disintegration tester.
Method: taking test sample 6, and in the glass tube for setting hanging basket respectively, starting disintegration tester is checked, each should all be
It is all disintegrated in 15min.It cannot be disintegrated completely if any 1, should separately take 6 retrials, regulation should all be met.
As a result: sample obtained by the embodiment of the present invention 1-4 meets regulation through slaking test inspection.
Friability inspection of the invention:
According to " Chinese Pharmacopoeia " 2010 editions (two) annex X G tablet friability inspection technique inspections.
Instrument: friability tester.
Method: if inspection technique slice weight is that 0.65g or following person take dry plate, making its gross weight is about 6.5g；Slice weight is greater than 0.65g
Person takes 10.The powder to fall off is blown away with hair dryer, precise weighing is set in cylinder, is rotated 100 times.It takes out, removes powder with method
End, precise weighing, less loss weight must not cross 1%, and must not detect the piece of fracture, cracking and crushing.This test generally only makees 1
It is secondary.When being more than 1% such as less loss weight, Ying Fujian 2 times, 3 average less loss weight must not cross 1%, and must not detect fracture,
Cracking and the piece crushed.
As a result: sample obtained by the embodiment of the present invention 1-4 meets regulation through friability test inspection.
Related substance of the invention
It is measured according to high performance liquid chromatography (two annex V D of Chinese Pharmacopoeia version in 2000).
Chromatographic condition and system suitability are filler with octadecylsilane chemically bonded silica；With methanol-water (80:
It 20) is mobile phase；Detection wavelength is 258nm, and column temperature is 35 DEG C.Number of theoretical plate should be not less than 5000 based on Anethol Trithione peak.
Measuring method takes test sample powder appropriate, sets in 100ml measuring bottle, and mobile phase is added to be made in every 1ml containing Anethol Trithione
The solution of 0.2mg/ml measures test solution 1ml as test solution, precision, and mobile phase is added to be made in every 1ml containing fennel three
The solution of sulphur 2mg/ml is as contrast solution；It is accurate respectively to draw test solution and each 10ml of contrast solution, inject liquid phase color
Spectrometer, 2 times of record chromatogram to main peak retention time.Such as aobvious impurity peaks of test solution, the sum of each impurity peak area must not
Greater than contrast solution main peak area (1.0%).
As a result: sample obtained by the embodiment of the present invention 1-4 meets regulation through Related substances separation.
Toxicity Analysis of the invention:
By severe combined immunodeficiency (SCID) mouse totally 40, liver cancer animal model is established by being inoculated with HepG2 cell,
The 14th day stochastic averagina is divided into four groups, respectively negative control group after modeling, 1 group of positive control, 2 groups of positive control, tests
Group.Positive controls and test group distinguish gastric infusion, 1 group of administration antrodia extract of positive control, and specification is 0.5ml/ times, and 3
Times/day；2 groups of administration Anethol Trithiones of positive control, specification are 1/ of Anethol Trithione content in antrodia Anethol Trithione composition described in embodiment 1
4,1/4 of antrodia Anethol Trithione composition antrodia content described in embodiment 1 is administered in test group, and specification is to be equivalent to antrodia extract
0.4ml/ times, 3 times/day；The change of each group mouse form and behavior is observed, modeling the 1st day, 7 days, 14 days, 21 days, 28 are chosen
It, be within 34 days time point, survey each group mouse blood SGPT (U/L) value with Lai Shi dynamic, and the 34th day whole mouse take after modeling
Liver is fixed with 10% formalin, makees PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM.
Each group mouse form behavior outcome:
Negative control group mouse and drowsiness, dispirited few dynamic, the fur shrinkage of modeling appearance in the 15th~17 day, back bow, gait are not
Surely, it the illness symptoms such as turn-takes.1 group of positive control occurred in modeling the 22nd~23 day drowsiness, dispirited few dynamic, fur shrinkage, back bow,
Instability of gait, the illness symptoms such as turn-take.2 groups of positive control occurred drowsiness, dispirited few dynamic, fur wrinkle in modeling the 18th~20 day
Contracting, back bow, instability of gait, the illness symptoms such as turn-take.There is the above illness symptom on the 26th~27 day in modeling in test group.
The detection of SGPT (U/L) value
Anesthesia: etherization mouse to eupnea, four limbs relaxation, 40~45 DEG C of warm water soak tail 20~30 seconds.
Blood sampling: dry cotton ball dries rat-tail, and scissors cuts tail point 2-4mm, and glass capillary syring draws 40 μ l blood, connects rubber
Cap is blown into rapidly in 1.65ml haemocyte dilution.
Detection method: reitman-frankel method.
SGPT (U/L) value result:
SGPT (U/L) value interpretation of result:
1 group of control of test group SGPT (U/L) value decline corresponding positive is slower, illustrates that composition drug effect of the invention compares camphor tree
Sesame extract is good to the therapeutic effect of liver cancer, and 1 group of SGPT (U/L) value decline corresponding positive of positive control compares 2 groups of slower, explanations
Antrodia extract has certain effect to the treatment of liver cancer, and 2 groups of positive control are not statistically significant with negative control group difference,
Therefore Anethol Trithione is to liver cancer without therapeutic effect.From SGPT (U/L) value interpretation of result it can be concluded that antrodia Anethol Trithione group of the invention
Closing object has apparent synergistic therapeutic action.
PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM
Mouse is taken off vertebra to put to death, liver is taken, is fixed with 10% formalin.
Negative control group: abdomen protuberance, abdomen can obviously touch lump as large as drum.Dissection is shown in that liver transfer operation tumor is rounded, quality
It is soft, it is grown in dilatancy, infiltration to liver organization, surface is distributed blood vessel abundant, and tumor weight increases to 2.26g.
1 group of positive control: abdomen protuberance, abdomen is big, can touch lump.Dissection is shown in that liver transfer operation tumor is rounded, and quality is soft, in expansion
Extensional growth, infiltration to liver organization, surface are distributed blood vessel abundant, and tumor weight increases to 1.71g.
2 groups of positive control: abdomen protuberance, abdomen can obviously touch lump as large as drum.Dissection is shown in that liver transfer operation tumor is rounded, matter
Ground is soft, grows in dilatancy, and infiltration to liver organization, surface is distributed blood vessel abundant, and tumor weight increases to 2.11g.
Test group: abdomen protuberance, abdomen is big, can touch lump.Dissection is shown in that liver transfer operation tumor is rounded, and quality is soft, is in dilatancy
Growth, infiltration to liver organization, surface are distributed blood vessel abundant, and tumor weight increases to 1.16g.
Interpretation of result:
1 group of control of test group liver transfer operation tumor growth corresponding positive is slower, illustrates that composition drug effect of the invention compares antrodia
Extract is good to the therapeutic effect of liver cancer, and 1 group of 2 groups of control of liver transfer operation tumor growth corresponding positive of positive control is slower, illustrates antrodia
Extract has certain effect to the treatment of liver cancer, and 2 groups of positive control not statistically significant with negative control group difference, therefore
Anethol Trithione is to liver cancer without therapeutic effect.From PATHOMORPHOLOGICAL OBSERVATION OF PULLORUM interpretation of result it can be concluded that antrodia Anethol Trithione of the invention combines
Object has apparent synergistic therapeutic action.
Embodiment described above only describe the preferred embodiments of the invention, not to model of the invention
It encloses and is defined, without departing from the spirit of the design of the present invention, those of ordinary skill in the art are to technical side of the invention
The various changes and improvements that case is made should all be fallen into the protection scope that claims of the present invention determines.
Claims (6)
1. a kind of antrodia Anethol Trithione composition for treating liver cancer, which is characterized in that the component and part of the composition is camphor tree
20~25 parts of sesame, 3~5 parts of Anethol Trithione, 15~20 parts of diluent, 0.5~1 part of surfactant, 15~20 parts of disintegrating agent, lubrication
0.1~0.5 part of agent；
The preparation method specific steps of the composition include:
1) it stocks up, stocks up according to the component and part of above-mentioned composition；
2) extraction of antrodia effective ingredient triterpene: crushed 200 meshes for antrodia, and 4 times of bodies of antrodia are added into ultrasonic extraction tank
Long-pending alcohol, alcohol quality concentration are 95%, smashed antrodia are added in ultrasonic extraction tank, ultrasonic 6h takes supernatant mark
It is denoted as a solution；Again into ultrasonic extraction tank be added 2 times of volumes of antrodia alcohol, alcohol quality concentration be 95%, ultrasonic 6h,
It takes supernatant labeled as b solution, merges solution a, b and obtain solution A, by solution A pressure is -0.06~-0.08Mpa, temperature is
/ 5th that solution A volume is concentrated under the conditions of 40~45 DEG C obtain concentrate B；
3) wet granulation: by concentrate B obtained by step 2) and a kind of other components for the antrodia Anethol Trithione composition for treating liver cancer
Wet granulation together, the drying equipment of wet granulation are fluidized bed, set the drying temperature of fluidized bed as 55~60 DEG C, and the time is
15~30min obtains particle C；
4) whole grain: particle C obtained by step 3) be crushed into 20~40 meshes and obtain particle D；
5) tabletting: particle D obtained by step 4) is subjected to tabletting by tablet press machine, slice weight is 0.5~0.8g.
2. a kind of antrodia Anethol Trithione composition for treating liver cancer according to claim 1, which is characterized in that the composition
Component and part are as follows: 22 parts of antrodia, 4 parts of Anethol Trithione, 18 parts of diluent, 0.7 part of surfactant, 17 parts of disintegrating agent, profit
0.3 part of lubrication prescription.
3. a kind of antrodia Anethol Trithione composition for treating liver cancer according to claim 1, which is characterized in that diluent are as follows:
At least one of Icing Sugar, dextrin, amylum pregelatinisatum, microcrystalline cellulose.
4. a kind of antrodia Anethol Trithione composition for treating liver cancer according to claim 1, which is characterized in that surfactant
Are as follows: fatty acid sorbitan, polysorbate, polyoxyethylene fatty acid ester, at least one in Pluronic F68
Kind.
5. a kind of antrodia Anethol Trithione composition for treating liver cancer according to claim 1, which is characterized in that disintegrating agent are as follows:
Sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crosslinked polyvinylpyrrolidone, in croscarmellose sodium at least
It is a kind of.
6. a kind of antrodia Anethol Trithione composition for treating liver cancer according to claim 1, which is characterized in that lubricant are as follows:
At least one of magnesium stearate, superfine silica gel powder, talcum powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510420369.2A CN105125594B (en) | 2015-07-16 | 2015-07-16 | A kind of antrodia Anethol Trithione composition that treating liver cancer and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510420369.2A CN105125594B (en) | 2015-07-16 | 2015-07-16 | A kind of antrodia Anethol Trithione composition that treating liver cancer and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105125594A CN105125594A (en) | 2015-12-09 |
| CN105125594B true CN105125594B (en) | 2019-04-09 |
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Citations (1)
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| CN1799560A (en) * | 2005-09-28 | 2006-07-12 | 莱阳农学院 | Liver cancer resistant Antrodia camphorata and preparation method thererof |
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| US20080242856A1 (en) * | 2007-04-02 | 2008-10-02 | Funci Biotechnology Co., Ltd. | Extracts of taiwanofungus camphoratus with a capacity for inhibiting the activity of matrix metalloproteinases and method for preparing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1799560A (en) * | 2005-09-28 | 2006-07-12 | 莱阳农学院 | Liver cancer resistant Antrodia camphorata and preparation method thererof |
Non-Patent Citations (2)
| Title |
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| 健脾活血中药联合茴三硫防治肝癌化疗栓塞术后肝功能损害的临床研究;邹雨荷等;《中国肿瘤临床与康复》;20060228;第13卷(第1期);第21-22,45页，尤其是第21页摘要 |
| 樟芝抗肝癌机制研究进展;陈清法等;《国际肿瘤学杂志》;20150630;第42卷(第6期);第469-471页，尤其是第469页左栏第1版 |
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