CN105112048A - Novel intracellular Golgi-complex fluorescence molecular probe and application thereof - Google Patents
Novel intracellular Golgi-complex fluorescence molecular probe and application thereof Download PDFInfo
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- CN105112048A CN105112048A CN201510514205.6A CN201510514205A CN105112048A CN 105112048 A CN105112048 A CN 105112048A CN 201510514205 A CN201510514205 A CN 201510514205A CN 105112048 A CN105112048 A CN 105112048A
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- molecular probe
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Abstract
The invention discloses a novel intracellular Golgi-complex fluorescence molecular probe. The probe has a structural formula described in the specification. The novel intracellular Golgi-complex fluorescence molecular probe provided by the invention has a fluorescent response region with a pH of acidic to neutral conditions and a pH range of 2 to 8, can realize transmembrane permeation into a cell, can emit fluorescence when combining with a Golgi complex, and is capable of realizing fluorescence detection or organelle imaging. The novel intracellular Golgi-complex fluorescence molecular probe provided by the invention has the advantages of cheap synthetic raw materials, simple synthetic steps and good combining imaging selectivity to the Golgi complex.
Description
Technical field
The present invention relates to fluorescent molecular probe technical field, particularly relate to golgi body fluorescent molecular probe and application thereof in a kind of novel cell.
Background technology
Cell is the organization unit that life entity owner wants, it is made up of various organoid again, as: cytolemma, cell walls, tenuigenin, chloroplast(id), golgi body, kernel, chromatin, nuclear membrane, karyolymph, nucleopore, plastosome, endoplasmic reticulum, rrna, enchylema, vacuole skin etc.Organoid plays important indicative function, as cell proliferation, apoptosis, resistance, ion transport, Muscle contraction etc. in cell, enzyme and a lot of tissue activity.To the growthhabit of organoid monitor for life cells behavioral study and neural system research significant.Some organoids, if kernel, rrna, endoplasmic reticulum are basophilia, and golgi body is acidophilia.
Golgi body (Golgiapparatus, Golgicomplex) is also known as Golgi complex, Golgi's organs.It is one of composition of endomembrane system in eukaryotic cell.It does not exist only in animal and plant cells, and is present in protozoon and fungal cell.
Fluorescence co-focusing imaging method is a kind of detection method set up based on optical signalling, have high sensitivity, highly selective, fast can the advantage such as visibility.But existing golgi body fluorescent molecular probe is commercially as Golgi-TrackerRed, expensive, one milligram is thousands of unit, and selectivity is not good in organoid imaging.
Summary of the invention
The present invention provides a kind of Novel fluorescent molecular probe to solve above-mentioned prior art Problems existing exactly.
Object of the present invention is achieved through the following technical solutions:
Golgi body fluorescent molecular probe in a kind of novel cell, has following structural formula:
The preparation feedback of this fluorescent molecular probe is shown below, and is undertaken by the Aldol condensation reaction condition of classics.
Preferably, the fluorescence response region of described fluorescent molecular probe is pH is acidity-neutrallty condition, and pH value range is 2-8.
The application of golgi body fluorescent molecular probe in a kind of above-mentioned cell.
Preferably, the solvent of the applying soln of described fluorescent molecular probe is selected from one or both in water, dimethyl sulfoxide (DMSO).
Preferably, the working concentration of described fluorescent molecular probe in described applying soln is 1 μM of-300mM.
Described fluorescent molecular probe can enter in cell by transmembranal penetration, can emitting fluorescence when described fluorescent molecular probe is attached to golgi body, carries out fluoroscopic examination or organoid imaging.
Compared with prior art, the present invention has following beneficial effect:
Novel golgi body fluorescent molecular probe synthesis material of the present invention is inexpensive, synthesis step simple, and finished product price is low, is combined into picture selectivity good to golgi body.
Accompanying drawing explanation
Fig. 1 is the typical golgi body confocal fluorescent image that the embodiment of the present invention 1 collects; Wherein (a) is fluorescence imaging figure, and (b) is light field figure.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
Golgi body fluorescent molecular probe in a kind of novel cell, has following molecular structural formula:
An embodiment of its preparation method is as follows:
Disubstituted-4-hydroxy phenyl aldehyde 1.12g (0.01mol) and pimelinketone 0.49g (0.005mol) joins in the reactor of the tetrahydrofuran solution containing 5% sulfuric acid, room temperature reaction 20h.After reaction terminates, silica gel column chromatography (ethyl acetate/petroleum ether gradient elution), obtains pale tan oil target-probe: two (4-phenol methylene) pimelinketone of 2,6-.The structure confirmation data of pale tan oil target-probe is as follows: ESIMS (ESI+): 307.15 (M+H); Ex=385nm, Em=520nm.
The application of this fluorescent molecular probe: this fluorescent molecular probe is scattered in the mixed solution (volume ratio 1:9) of dimethyl sulfoxide (DMSO) and water with the concentration of 20 μMs, 1 hour is hatched altogether with human breast cancer cell, 60 times of object lens, with 385nm optical excitation, imaging is carried out to the confocal fluorescence image at 520nm place, obtains golgi body fluorescence imaging figure as shown in Figure 1.Result is shown, only acidophilia golgi cell utensil fluorescence, other non-eosinophil device then unstressed configuration from figure.Further, contriver verifies through many experiments, determines that the working concentration of golgi body fluorescent molecular probe in novel cell of the present invention is 1 μM of-300mM.
In novel cell of the present invention, preparation method and application's example of golgi body fluorescent molecular probe is only citing above; those skilled in the art also can make various deformation embodiment in above-mentioned scope of disclosure, and these variant embodiment are all in protection scope of the present invention.
Under the instruction of the present invention and above-described embodiment, those skilled in the art are easy to predict, cited or each raw material that exemplifies of the present invention or its equivalent alterations, each working method or its equivalent alterations can realize the present invention, and the parameter bound value of each raw material and working method, interval value can realize the present invention, do not enumerate embodiment at this.
Be only several specific embodiments of the application above, but the application is not limited thereto, the changes that any person skilled in the art can think of, all should drops in the protection domain of the application.
Claims (7)
1. a golgi body fluorescent molecular probe in cell, is characterized in that, has following structural formula:
2. golgi body fluorescent molecular probe in cell as claimed in claim 1, it is characterized in that, in described cell, the fluorescence response region of golgi body fluorescent molecular probe is pH is acidity-neutrallty condition, and pH value range is 2-8.
3. golgi body fluorescent molecular probe in cell as claimed in claim 1, it is characterized in that, in described cell, golgi body fluorescent molecular probe can enter in cell by transmembranal penetration.
4. golgi body fluorescent molecular probe in cell as claimed in claim 1, is characterized in that, can emitting fluorescence when in described cell, golgi body fluorescent molecular probe is attached to golgi body, can carry out fluoroscopic examination or organoid imaging.
5. the application of golgi body fluorescent molecular probe in arbitrary described cell in a claim 1-4.
6. the application of golgi body fluorescent molecular probe in cell as claimed in claim 5, it is characterized in that, in described cell, the solvent of the applying soln of golgi body fluorescent molecular probe is selected from one or both in water, dimethyl sulfoxide (DMSO).
7. golgi body fluorescent molecular probe in cell as claimed in claim 6, it is characterized in that, in described applying soln, in described cell, the working concentration of golgi body fluorescent molecular probe is 1 μM of-300mM.
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| CN201510514205.6A CN105112048A (en) | 2015-08-20 | 2015-08-20 | Novel intracellular Golgi-complex fluorescence molecular probe and application thereof |
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| CN201510514205.6A CN105112048A (en) | 2015-08-20 | 2015-08-20 | Novel intracellular Golgi-complex fluorescence molecular probe and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093153A (en) * | 2019-05-30 | 2019-08-06 | 山东师范大学 | A kind of positioning golgiosome near infrared fluorescent probe and its synthetic method and application |
| CN110885675A (en) * | 2019-11-22 | 2020-03-17 | 山东师范大学 | Nano fluorescent probe, preparation method and application thereof in detection of HNO in Golgi apparatus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102557969A (en) * | 2011-11-01 | 2012-07-11 | 中国科学技术大学 | Cyclic ketone derivatives and applications thereof as developers and aggregation inhibitors of amyloid protein sediments and neurofibrillary tangles |
| US20140271470A1 (en) * | 2013-03-13 | 2014-09-18 | Laurel O. Sillerud | Targeted theranostics for metastatic prostate cancer |
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2015
- 2015-08-20 CN CN201510514205.6A patent/CN105112048A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102557969A (en) * | 2011-11-01 | 2012-07-11 | 中国科学技术大学 | Cyclic ketone derivatives and applications thereof as developers and aggregation inhibitors of amyloid protein sediments and neurofibrillary tangles |
| US20140271470A1 (en) * | 2013-03-13 | 2014-09-18 | Laurel O. Sillerud | Targeted theranostics for metastatic prostate cancer |
Non-Patent Citations (1)
| Title |
|---|
| D. XIANG ET AL.，: ""The study of acurcumin-resembling molecular probe for the pH-responsive fluorometric assay and application in cell imaging"", 《TALANTA》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110093153A (en) * | 2019-05-30 | 2019-08-06 | 山东师范大学 | A kind of positioning golgiosome near infrared fluorescent probe and its synthetic method and application |
| CN110093153B (en) * | 2019-05-30 | 2022-02-08 | 山东师范大学 | Positioning Golgi body near-infrared fluorescent probe and synthetic method and application thereof |
| CN110885675A (en) * | 2019-11-22 | 2020-03-17 | 山东师范大学 | Nano fluorescent probe, preparation method and application thereof in detection of HNO in Golgi apparatus |
| CN110885675B (en) * | 2019-11-22 | 2022-07-12 | 山东师范大学 | Nano fluorescent probe, preparation method and application thereof in detection of HNO in Golgi apparatus |
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