CN105092675A - Citric acid concentration detection apparatus and detection method - Google Patents
Citric acid concentration detection apparatus and detection method Download PDFInfo
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- CN105092675A CN105092675A CN201510067410.2A CN201510067410A CN105092675A CN 105092675 A CN105092675 A CN 105092675A CN 201510067410 A CN201510067410 A CN 201510067410A CN 105092675 A CN105092675 A CN 105092675A
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Abstract
The present invention discloses a citric acid concentration detection apparatus and a detection method. The citric acid concentration detection apparatus is electrically connected to a computer; the apparatus includes a test stand, a test tray, and an acoustic surface wave resonator, an electronic counter, an oscillator and a cell electrode chip, which are located on the test stand. The oscillator and the acoustic surface wave resonator from an oscillation circuit; the counter is electrically connected to the oscillation circuit; the acoustic surface wave resonator is electrically connected to the cell electrode chip; and the computer is electrically connected to the counter. The present invention has the characteristics of simple operation, good detection sensitivity, high precision, high detection speed, good device stability, and good repeatability.
Description
Technical field
The present invention relates to solution concentration detection technical field, especially relate to a kind of detection accuracy high, fireballing citric acid concentration pick-up unit and detection method.
Background technology
Existing solution concentration detection method has instrumental analysis detection method and chemical measure, although instrumental analysis detection method is simple to operate, there is the defect that accuracy of detection is low; There is complex operation in chemical measure, the deficiency of poor repeatability.
Chinese patent mandate publication number: CN103528877A, authorizes publication date on January 22nd, 2014, discloses the online system of detecting concentration of sugar of a kind of miniature biochemical reactor, comprise 1 touch-screen, be responsible for the setting and the operation that accept field staff; 2 constant flow pumps, are responsible for diluting reactant liquor; 1 online sugar analyzer, is responsible for the on-line checkingi of sugared concentration; 1 off-line sugar concentration analyzer, is responsible for the offline inspection of sugared concentration; 1 flush bonding module BL2100, as the control module of system, control the flow velocity of constant flow pump and the action of sugared concentration analyzer, gather the sugared Concentration Testing value of online glycan analysis instrument, on-line correction is carried out to collection value, to obtain in biochemical reactor sugared Concentration Testing value accurately, and according to the value of off-line sugar concentration analyzer, model parameter is revised.The weak point of this invention is, the deficiency of the low and poor repeatability of accuracy of detection.
Summary of the invention
Goal of the invention of the present invention is the deficiency in order to overcome the low and poor repeatability of detection method accuracy of detection of the prior art, provides a kind of detection accuracy high, fireballing citric acid concentration pick-up unit and detection method.
To achieve these goals, the present invention is by the following technical solutions:
A kind of citric acid concentration pick-up unit, described citric acid concentration pick-up unit is electrically connected with computing machine; Comprise testing table, detect pallet, be located at the SAW (Surface Acoustic Wave) resonator on testing table, electronic counter, oscillator and cell electrode chip; Oscillator and SAW (Surface Acoustic Wave) resonator form oscillation circuit, and counter is electrically connected with oscillation circuit, and SAW (Surface Acoustic Wave) resonator is electrically connected with cell electrode chip, and counter is electrically connected with computing machine;
Described cell electrode chip comprise ST cut type quartz make substrate, be located at upper surface of base plate palladium to electrode, palladium contrast electrode and working electrode; Described cell electrode chip lies against in the groove of detection supporting plate, and bottom portion of groove is provided with several liquid outlets, and groove is connected with experiment table by several supporting pads; Described working electrode comprises foam layers of copper and is located at the sputtering layer gold in foam layers of copper, is equipped with mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells in each mesh of sputtering layer gold; Base lower surface is provided with copper plate;
Described testing table is provided with L shape sway brace, and sway brace is provided with disk, and disk is provided with the transfusion bottle that n fills the citric acid solution of variable concentrations respectively; The container of the upper end open be positioned at below disk is provided with in the middle part of sway brace, the stirring apparatus be connected with draft tube is provided with in container, stirring apparatus is provided with several ventholes, and lower vessel portion is provided with drain pipe, and container lower end is provided with the micro pump of the citric acid solution that can just drip to working electrode; Sway brace is connected with disk by the motor that disc level can be driven to rotate, and each transfusion bottle lower end, drain pipe and draft tube are equipped with solenoid valve, and computing machine is electrically connected with micro pump, motor and each solenoid valve respectively.
The preparation process of described cell electrode is as follows:
Be provided with sputter, target disc holder, electrode supporting plate successively for sputtering in the golden sputtering chamber processed, electrode supporting plate is connected with sputtering chamber by the horizontal rotating shaft of the first motor, and target disc holder is connected with sputtering chamber by the horizontal rotating shaft of the second motor; Sputtering chamber is also provided with watch window; The horizontal rotating shaft of the first motor and the horizontal rotating shaft of the second motor coaxial;
(1-1) by purity >=99.99%, diameter is 4.5 to 5.0cm, thickness is that the gold target dish of 0.5 to 0.7cm is installed on target disc holder, by comprise ST cut type quartz make substrate, be located at the electrode chip of the working electrode that electrode, contrast electrode and foam copper material are made is installed on electrode supporting plate on substrate, make working electrode towards target disc and expose outside electrode supporting plate;
(1-2) utilize ionic pump to vacuumize sputtering chamber, make vacuum tightness reach 4.2 × 10
-5below Pa; The argon gas of purity>=99.99% is filled with to sputtering chamber;
(1-3) make the power of sputter be that staged declines gradually by W, the gold atom on target disc is splashed on working electrode; In the process of sputtering, the second driven by motor target disc at the uniform velocity rotates, and the first driven by motor electrode chip is with the first rotating Vortex of the rotating speed being greater than the second motor, then reverse rotation, and then rotating Vortex; Obtain the electrode chip after sputtering gold;
(1-4), after the electrode chip after sputtering gold being carried out drying, immersion, sterilization processing, the cell suspension made is dripped on working electrode, cultivate 2 to 3 hours, obtain cell electrode with mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells.
The working electrode of cell electrode chip of the present invention adopts foam copper material to make, foam copper has equally distributed three-dimensional netted pore structure, the reticulate texture of multi-layer stable connects closely, the satisfactory mechanical property of copper, reticulate texture can be prevented to be out of shape, to subside and the fracture on surface may.The diameter of working electrode surface mesh is between 0.15mm-0.35mm, and the mesh diameter of its inside is more and more less, this special structure is conducive to molecule or ion moves freely in mesh, make the electroactive molecule of tool or ion can enter foam copper inside configuration, increase the contact area of solution to be measured and electrode, improve sensitivity and the selectivity of working electrode.
The present invention sputters gold atom on the working electrode (s further, thus in reticulate texture, add one deck gold film, gold film has the effect of insulation foam copper and mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells, because copper is to the virulent effect of mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells tool, the activity of mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells can be reduced, and gold does not have toxic action to mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells, mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells is active better, add the biocompatibility of working electrode, further increase the sensitivity of detection.
Biological tongue of the present invention is made up of the mouse bacterium shape taste bud tart flavour taste receptor cells cultivated on the working electrode (s, because mouse bacterium shape taste bud tart flavour taste receptor cells has sensitive response to citric acid, mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells is cultivated at foam copper electrode surface as sense of taste sensitive element, the stimulation that cell is subject to taste material molecule produces specificly-response, discharge the neurotransmitter such as ATP and serotonin chemical substance simultaneously, these materials have reductibility, specificity current-responsive is obtained by foam copper anodizing, current-responsive is captured by surface acoustic wave series resonator, detect with the form of frequency, according to SAW (Surface Acoustic Wave) resonator detect frequency just can realize taste material information detect target.The method of reducing of the copper plate that base lower surface is provided with to the neurotransmitter such as ATP and serotonin chemical substance has catalytic action, increases specificity current-responsive value, further increases detection sensitivity.
Therefore, the present invention has simple to operate, and detection sensitivity is good, and accuracy of detection is high, and detection speed is fast; Device stability is good, reproducible feature.
As preferably, described stirring apparatus is the T-shaped stirring pipe be located in lower vessel portion, column is provided with in described container, column coboundary is provided with the flange of outside horizontal-extending, the lower limb of T-shaped stirring pipe is provided with the annular edge of the inside horizontal-extending suitable with flange, annular edge upper surface and flange lower surface clearance fit;
Column top is provided with back-up ring, and back-up ring upper surface contacts with annular edge lower surface; Column bottom is connected with draft tube;
Each venthole lays respectively at the outer peripheral face rear portion of T-shaped stirring pipe one end and the outer peripheral face front portion of the other end.
T-shaped stirring pipe can stir while be filled with dry air in solution, thus improves the air concentration in solution, and the solution of rich oxygen content can improve the activity of mouse bacterium shape taste bud taste receptor cells, and cytoactive is good, then the sensitivity detected is higher.
As preferably, the xsect ovalize of described T-shaped stirring pipe, the outer peripheral face upper and lower of T-shaped stirring pipe is equipped with several ventholes.
As preferably, SAW (Surface Acoustic Wave) resonator comprises piezoelectric substrate, interdigital transducer, 2 reflecting gratings, is located at 4 gain grid of reflecting grating both sides and is arranged at two sound absorption parts away from interdigital transducer side of two reflecting gratings respectively; Electrode and working electrode are electrically connected with 2 links be located on interdigital transducer respectively.
A detection method for citric acid concentration pick-up unit, comprises the steps:
(5-1) in n transfusion bottle of disk, fill the citric acid solution that concentration raises gradually successively, computing machine according to the order that concentration raises be each transfusion bottle by 1 to n number consecutively, store the citric acid solution concentration k in 1 to n transfusion bottle in computing machine
1, k
2..., k
n, the initial value of transfusion bottle sequence number i is 1, i=1 ..., n;
(5-2) closed electromagnetic valve of computer controlled drain pipe processed, by Electric Machine Control disk rotational, the transfusion bottle being numbered i is aimed at container, and the solenoid valve that computing machine controls this transfusion bottle is opened, and the citric acid solution in transfusion bottle is poured in container;
(5-3) computing machine controls to open with the solenoid valve of the draft tube of air communication, and stirring apparatus is filled with the dry air that temperature is 30 to 35 DEG C while stirring in solution, and after 5 to 9 minutes, computing machine controls the closed electromagnetic valve of draft tube;
(5-4) computing machine controls micro pump per interval T and to drip to working electrode the citric acid solution of 0.05ml, and after dripping off 295 to 308, computing machine controls micro pump and stops dropping liquid; While dropping liquid, counter gathers the frequency response curve of oscillation circuit, and computing machine gathers several frequency values on frequency response curve, and each frequency values is formed frequency signal S (t);
(5-5) second-order linearity accidental resonance model is preset with in computing machine
S (t) is inputted second-order linearity accidental resonance model and second-order linearity accidental resonance model is resonated;
Wherein, x (t) is the displacement of vibration particle, and Ω is angular frequency, r and ω is the attenuation coefficient of setting and the frequency of linear oscillator particle respectively, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting
2t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p
s(a)=p
s(-a)=q, p
s(0)=1-2q, wherein 0 < q < 0.5;
Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa
2e
-λ τ;
Wherein λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is
When model resonates, particle produces resonance in certain position, and this angular frequency Ω, attenuation coefficient r, correlation ratio λ, coefficient b, constant a, q determine;
Computing machine utilizes formula
calculate and obtain output signal-to-noise ratio SNR
i; Computer inquery is numbered the concentration k of the citric acid solution of i
i, and by k
iand SNR
ibe stored in computing machine;
(5-6) as i < n, make i value increase by 1, the solenoid valve of computer controlled drain pipe processed is opened, and flows out in unnecessary solution from container, proceeds to step (5-2); Otherwise, proceed to step (5-7);
(5-7) computing machine reads the citric acid solution concentration k stored
1, k
2..., k
n; Computing machine utilizes point (k
1, SNR
1), (k
2, SNR
2) ..., (k
n, SNR
n) fit to straight line, draw citric acid concentration forecast model according to the straight line of matching:
(5-8) by micro pump and the reservoir filling citric acid solution W to be detected, and (5-2) detection is utilized to obtain frequency signal S (t)
w, by S (t)
wthe second-order linearity accidental resonance model of input step (5-5), makes second-order linearity accidental resonance model resonate;
And utilize formula
calculate and obtain the output signal-to-noise ratio SNR corresponding with citric acid solution
w;
(5-9) by SNR
wsubstitute into
in, calculate the concentration k of citric acid solution W
w.
As preferably, described T is 0.9 to 1.4 second.
As preferably, described n is 8 to 17.
As preferably, step (5-4) Computer gathers 95 to 118 frequency values be spacedly distributed on frequency response curve.
The present invention is the citric acid solution by detecting concentration known, obtains citric acid concentration forecast model, and detects the concentration of the citric acid solution of unknown concentration with citric acid concentration forecast model; The stability, the good reliability that detect, highly sensitive, detection speed is fast.
Therefore, the present invention has following beneficial effect:
(1) simple to operate, detection sensitivity is good, and accuracy of detection is high, and detection speed is fast;
(2) device stability is good, reproducible.
Accompanying drawing explanation
Fig. 1 is a kind of theory diagram of the present invention;
Fig. 2 is a kind of structural representation of detection pallet of the present invention;
Fig. 3 is a kind of structural representation of SAW (Surface Acoustic Wave) resonator of the present invention;
Fig. 4 is a kind of structural representation of cell electrode chip of the present invention;
Fig. 5 is a kind of process flow diagram of embodiments of the invention;
Fig. 6 is a kind of structural representation of the present invention;
Fig. 7 is a kind of structural representation of T-shaped stirring pipe of the present invention, column and back-up ring.
In figure: SAW (Surface Acoustic Wave) resonator 1, electronic counter 2, oscillator 3, cell electrode chip 4, computing machine 5, substrate 6, to electrode 7, contrast electrode 8, working electrode 9, detect pallet 10, micro pump 11, liquid outlet 12, bracing frame 13, container 14, testing table 15, sway brace 16, disk 17, transfusion bottle 18, stirring apparatus 19, venthole 20, piezoelectric substrate 21, interdigital transducer 22, reflecting grating 23, gain grid 24, sound absorption part 25, link 26, painting layer 27, drain pipe 28, motor 29, solenoid valve 30, T-shaped stirring pipe 31, column 32, flange 33, annular edge 34, back-up ring 35.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment is as shown in Figure 1 a kind of citric acid concentration pick-up unit, and described citric acid concentration pick-up unit is electrically connected with computing machine; Comprise testing table, detect pallet, be located at the SAW (Surface Acoustic Wave) resonator 1 on testing table, electronic counter 2, oscillator 3 and cell electrode chip 4; Oscillator and SAW (Surface Acoustic Wave) resonator form oscillation circuit, and counter is electrically connected with oscillation circuit, and SAW (Surface Acoustic Wave) resonator is electrically connected with cell electrode chip, and counter is electrically connected with computing machine 5;
As shown in Figure 4, cell electrode chip comprise ST cut type quartz make substrate 6, be located at upper surface of base plate palladium to electrode 7, palladium contrast electrode 8 and working electrode 9; Contrast electrode is connected with working electrode by 5V bias direct current voltage.To electrode, contrast electrode and working electrode upper surface all higher than upper surface of base plate 1 centimetre.
As shown in Figure 2, cell electrode chip lies against in the groove of detection supporting plate 10, and bottom portion of groove is provided with 5 liquid outlets 12, and groove is connected with experiment table by 4 supporting pads; Working electrode comprises foam layers of copper and is located at the sputtering layer gold in foam layers of copper, is equipped with mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells in each mesh of sputtering layer gold; Base lower surface is provided with copper plate;
As shown in Figure 7, testing table 15 is provided with L shape sway brace 16, and sway brace is provided with disk 17, and disk is provided with the transfusion bottle 18 that 15 fill the citric acid solution of variable concentrations respectively; Be provided with the container 14 of the upper end open be positioned at below disk in the middle part of sway brace, be provided with the stirring apparatus 19 be connected with draft tube in container, stirring apparatus is provided with 35 ventholes 20; Lower vessel portion is provided with drain pipe 28, and container lower end is provided with the micro pump 11 of the citric acid solution that can just drip to working electrode; Sway brace is connected with disk by the motor 29 that disc level can be driven to rotate, and each transfusion bottle lower end, drain pipe and draft tube are equipped with solenoid valve 30; As shown in Figure 1, computing machine is electrically connected with micro pump, motor and each solenoid valve respectively.
As shown in Figure 3, SAW (Surface Acoustic Wave) resonator comprises piezoelectric substrate 21, interdigital transducer 22,2 reflecting gratings 23, is located at 4 gain grid 24 of reflecting grating both sides and is arranged at two sound absorption parts 25 away from interdigital transducer side of two reflecting gratings respectively; Electrode and working electrode are electrically connected with 2 links 26 be located on interdigital transducer respectively.
As shown in Figure 7, stirring apparatus is the T-shaped stirring pipe 31 be located in lower vessel portion, column 32 is provided with in described container, column coboundary is provided with the flange 33 of outside horizontal-extending, the lower limb of T-shaped stirring pipe is provided with the annular edge 34 of the inside horizontal-extending suitable with flange, annular edge upper surface and flange lower surface clearance fit; Column top is provided with back-up ring 35, and back-up ring upper surface contacts with annular edge lower surface; Column bottom is connected with draft tube; Each venthole lays respectively at the outer peripheral face rear portion of T-shaped stirring pipe one end and the outer peripheral face front portion of the other end.
The xsect ovalize of T-shaped stirring pipe, the outer peripheral face upper and lower of T-shaped stirring pipe is equipped with 10 ventholes.
As shown in Figure 5, a kind of detection method of citric acid concentration pick-up unit, comprises the steps:
Step 100 is transfusion bottle numbering
The citric acid solution that concentration raises gradually is filled successively in 15 transfusion bottles of disk, computing machine according to the order that concentration raises be each transfusion bottle by 1 to 15 number consecutively, store the citric acid solution concentration k in 1 to 15 transfusion bottle calculated according to allotment ratio in computing machine
1, k
2..., k
15, the initial value of transfusion bottle sequence number i is 1, i=1 ..., 15;
Step 200, pours into the solution be numbered in the transfusion bottle of i in container
The closed electromagnetic valve of computer controlled drain pipe processed, by Electric Machine Control disk rotational, the transfusion bottle being numbered i is aimed at container, and the solenoid valve that computing machine controls this transfusion bottle is opened, and the citric acid solution in transfusion bottle is poured in container;
Step 300, stirs and blowing air the solution in container
Computing machine controls to open with the solenoid valve of the draft tube of air communication, and stirring apparatus is filled with the dry air that temperature is 35 DEG C while stirring in solution, and after 5 to 9 minutes, computing machine controls the closed electromagnetic valve of draft tube;
Step 400, detects
Computing machine controls micro pump per interval T and to drip to working electrode the citric acid solution of 0.05ml, and after dripping off 300, computing machine controls micro pump and stops dropping liquid; While dropping liquid, counter gathers the frequency response curve of oscillation circuit, and computing machine gathers several frequency values on frequency response curve, and each frequency values is formed frequency signal S (t);
Step 500, data processing
Second-order linearity accidental resonance model is preset with in computing machine
S (t) is inputted second-order linearity accidental resonance model and second-order linearity accidental resonance model is resonated;
Wherein, x (t) is the displacement of vibration particle, and Ω is angular frequency, r and ω is the attenuation coefficient of setting and the frequency of linear oscillator particle respectively, and c is the signal adjustment coefficient of setting, and b is the quadratic noise ξ of setting
2t the coefficient of (), ξ (t) is three discrimination noises, and { dismutation of noise follows Poisson distribution to ξ (t) ∈ for-a, 0, a}, a > 0, and its probability distribution is p
s(a)=p
s(-a)=q, p
s(0)=1-2q, wherein 0 < q < 0.5;
Noise average and correlativity follow < ξ (t) >=0, < ξ (t) ξ (t+ τ) >=2qa
2e
-λ τ;
Wherein λ is correlation ratio, and the flatness of three discriminations noise ξ (t) is
Computing machine utilizes formula
calculate and obtain output signal-to-noise ratio SNR
i; Computer inquery is numbered the concentration k of the citric acid solution of i
i, and by k
iand SNR
ibe stored in computing machine;
Step 600, cycle detection
As i < 15, make i value increase by 1, the solenoid valve of computer controlled drain pipe processed is opened, and flows out, proceed to step 200 in unnecessary solution from container; Otherwise, proceed to step 700;
Step 700, draws citric acid concentration forecast model
Computing machine reads the citric acid solution concentration k stored
1, k
2..., k
15; Computing machine utilizes point (k
1, SNR
1), (k
2, SNR
2) ..., (k
15, SNR
15) fit to straight line, draw citric acid concentration forecast model according to the straight line of matching:
Step 800, detects the citric acid solution W of unknown concentration
By micro pump and the reservoir filling citric acid solution W to be detected, and step 200 detection is utilized to obtain frequency signal S (t)
w, by S (t)
wthe second-order linearity accidental resonance model of input step 500, makes second-order linearity accidental resonance model resonate;
And utilize formula
calculate and obtain the output signal-to-noise ratio SNR corresponding with citric acid solution
w;
Step 900, obtains the concentration of citric acid solution W
By SNR
wsubstitute into
in, calculate the concentration k of citric acid solution W
w.
In the present embodiment, T is 1 second, and step 500 Computer gathers 100 frequency values be spacedly distributed on frequency response curve.
Should be understood that the present embodiment is only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Claims (8)
1. a citric acid concentration pick-up unit, described citric acid concentration pick-up unit is electrically connected with computing machine; It is characterized in that, comprise testing table, detect pallet, be located at the SAW (Surface Acoustic Wave) resonator (1) on testing table, electronic counter (2), oscillator (3) and cell electrode chip (4); Oscillator and SAW (Surface Acoustic Wave) resonator form oscillation circuit, and counter is electrically connected with oscillation circuit, and SAW (Surface Acoustic Wave) resonator is electrically connected with cell electrode chip, and counter is electrically connected with computing machine (5);
Described cell electrode chip comprise ST cut type quartz make substrate (6), be located at upper surface of base plate palladium to electrode (7), palladium contrast electrode (8) and working electrode (9); Described cell electrode chip lies against in the groove of detection supporting plate (10), and bottom portion of groove is provided with several liquid outlets (12), and groove is connected with experiment table by several supporting pads; Described working electrode comprises foam layers of copper and is located at the sputtering layer gold in foam layers of copper, is equipped with mouse lingual surface bacterium shape taste bud tart flavour taste receptor cells in each mesh of sputtering layer gold; Base lower surface is provided with copper plate;
Described testing table (15) is provided with L shape sway brace (16), and sway brace is provided with disk (17), and disk is provided with the transfusion bottle (18) that n fills the citric acid solution of variable concentrations respectively; The container (14) of the upper end open be positioned at below disk is provided with in the middle part of sway brace, the stirring apparatus (19) be connected with draft tube is provided with in container, stirring apparatus is provided with several ventholes (20), lower vessel portion is provided with drain pipe (28), and container lower end is provided with the micro pump (11) of the citric acid solution that can just drip to working electrode; Sway brace is connected with disk by the motor (29) that disc level can be driven to rotate, each transfusion bottle lower end, drain pipe and draft tube are equipped with solenoid valve (30), computing machine is electrically connected with micro pump, motor and each solenoid valve respectively.
2. citric acid concentration pick-up unit according to claim 1, it is characterized in that, described stirring apparatus is the T-shaped stirring pipe (31) be located in lower vessel portion, column (32) is provided with in described container, column coboundary is provided with the flange (33) of outside horizontal-extending, the lower limb of T-shaped stirring pipe is provided with the annular edge (34) of the inside horizontal-extending suitable with flange, annular edge upper surface and flange lower surface clearance fit;
Column top is provided with back-up ring (35), and back-up ring upper surface contacts with annular edge lower surface; Column bottom is connected with draft tube;
Each venthole lays respectively at the outer peripheral face rear portion of T-shaped stirring pipe one end and the outer peripheral face front portion of the other end.
3. citric acid concentration pick-up unit according to claim 2, is characterized in that, the xsect ovalize of described T-shaped stirring pipe, and the outer peripheral face upper and lower of T-shaped stirring pipe is equipped with several ventholes.
4. the citric acid concentration pick-up unit according to claim 1 or 2 or 3, it is characterized in that, SAW (Surface Acoustic Wave) resonator comprises piezoelectric substrate, interdigital transducer, 2 reflecting gratings, is located at 4 gain grid of reflecting grating both sides and is arranged at two sound absorption parts away from interdigital transducer side of two reflecting gratings respectively; Electrode and working electrode are electrically connected with 2 links be located on interdigital transducer respectively.
5. be applicable to a detection method for citric acid concentration pick-up unit according to claim 1, it is characterized in that, comprise the steps:
(5-1) in n transfusion bottle of disk, fill the citric acid solution that concentration raises gradually successively, computing machine according to the order that concentration raises be each transfusion bottle by 1 to n number consecutively, store the citric acid solution concentration k in 1 to n transfusion bottle in computing machine
1, k
2..., k
n, the initial value of transfusion bottle sequence number i is 1, i=1 ..., n;
(5-2) closed electromagnetic valve of computer controlled drain pipe processed, by Electric Machine Control disk rotational, the transfusion bottle being numbered i is aimed at container, and the solenoid valve that computing machine controls this transfusion bottle is opened, and the citric acid solution in transfusion bottle is poured in container;
(5-3) computing machine controls to open with the solenoid valve of the draft tube of air communication, and stirring apparatus is filled with the dry air that temperature is 30 to 35 DEG C while stirring in solution, and after 5 to 9 minutes, computing machine controls the closed electromagnetic valve of draft tube;
(5-4) computing machine controls micro pump per interval T and to drip to working electrode the citric acid solution of 0.05ml, and after dripping off 295 to 308, computing machine controls micro pump and stops dropping liquid; While dropping liquid, counter gathers the frequency response curve of oscillation circuit, and computing machine gathers several frequency values on frequency response curve, and each frequency values is formed frequency signal S (t);
(5-5) second-order linearity accidental resonance model is preset with in computing machine
S (t) is inputted second-order linearity accidental resonance model and second-order linearity accidental resonance model is resonated;
Computing machine utilizes formula
calculate and obtain output signal-to-noise ratio SNR
i; Computer inquery is numbered the concentration k of the citric acid solution of i
i, and by k
iand SNR
ibe stored in computing machine;
(5-6) as i < n, make i value increase by 1, the solenoid valve of computer controlled drain pipe processed is opened, and flows out in unnecessary solution from container, proceeds to step (5-2); Otherwise, proceed to step (5-7);
(5-7) computing machine reads the citric acid solution concentration k stored
1, k
2..., k
n; Computing machine utilizes point (k
1, SNR
1), (k
2, SNR
2) ..., (k
n, SNR
n) fit to straight line, draw citric acid concentration forecast model according to the straight line of matching:
(5-8) by micro pump and the reservoir filling citric acid solution W to be detected, and (5-2) detection is utilized to obtain frequency signal S (t)
w, by S (t)
wthe second-order linearity accidental resonance model of input step (5-5), makes second-order linearity accidental resonance model resonate;
And utilize formula
calculate and obtain the output signal-to-noise ratio SNR corresponding with citric acid solution
w;
(5-9) by SNR
wsubstitute into
in, calculate the concentration k of citric acid solution W
w.
6. the detection method of citric acid concentration pick-up unit according to claim 5, is characterized in that, described T is 0.9 to 1.4 second.
7. the detection method of citric acid concentration pick-up unit according to claim 5, is characterized in that, described n is 8 to 17.
8. the detection method of the citric acid concentration pick-up unit according to claim 5 or 6 or 7 or 8, it is characterized in that, step (5-4) Computer gathers 95 to 118 frequency values be spacedly distributed on frequency response curve.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110320268A (en) * | 2019-08-15 | 2019-10-11 | 杭州市中医院 | The method for characterizing the taste substance in solution by sense of taste detection system |
| CN113008955A (en) * | 2021-03-03 | 2021-06-22 | 浙江农林大学 | Device and method for detecting concentration of glucose, fructose and sucrose mixed solution |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040191918A1 (en) * | 2003-03-28 | 2004-09-30 | Sandrine Isz | Evaluation of bitterness of active drugs |
| CN103424525A (en) * | 2013-03-18 | 2013-12-04 | 浙江工商大学 | Milk quality detection equipment and method |
| CN104089996A (en) * | 2014-01-17 | 2014-10-08 | 浙江工商大学 | Taste cell sensor using surface acoustic wave resonator and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040191918A1 (en) * | 2003-03-28 | 2004-09-30 | Sandrine Isz | Evaluation of bitterness of active drugs |
| CN103424525A (en) * | 2013-03-18 | 2013-12-04 | 浙江工商大学 | Milk quality detection equipment and method |
| CN104089996A (en) * | 2014-01-17 | 2014-10-08 | 浙江工商大学 | Taste cell sensor using surface acoustic wave resonator and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110320268A (en) * | 2019-08-15 | 2019-10-11 | 杭州市中医院 | The method for characterizing the taste substance in solution by sense of taste detection system |
| CN113008955A (en) * | 2021-03-03 | 2021-06-22 | 浙江农林大学 | Device and method for detecting concentration of glucose, fructose and sucrose mixed solution |
| CN113008955B (en) * | 2021-03-03 | 2023-03-17 | 浙江农林大学 | Device and method for detecting concentration of glucose, fructose and sucrose mixed solution |
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