CN105087542A - Environmental stress sensitive type immobilized enzyme porous micro-sphere carrier material and preparing method of porous micro-sphere carrier material - Google Patents
Environmental stress sensitive type immobilized enzyme porous micro-sphere carrier material and preparing method of porous micro-sphere carrier material Download PDFInfo
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- CN105087542A CN105087542A CN201510593230.8A CN201510593230A CN105087542A CN 105087542 A CN105087542 A CN 105087542A CN 201510593230 A CN201510593230 A CN 201510593230A CN 105087542 A CN105087542 A CN 105087542A
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Abstract
The invention discloses an environmental stress sensitive type immobilized enzyme porous micro-sphere carrier material and a preparing method of the porous micro-sphere carrier material. The porous micro-sphere carrier material comprises the following ingredients in parts by weight: 22 to 26 parts of starch, 20 to 30 parts of oyster shells, 18 to 20 parts of dextran, 14 to 16 parts of nano calcium carbonate, 7 to 9 parts of ammonium bicarbonate solution, 12 to 16 parts of positively charged polylysine, 5 to 7 parts of ferrofluid, 14 to 18 parts of polyion liquid, 3 to 5 parts of hydroxymethyl cellulose, 2 to 3 parts of amidated low methoxyl pectin, 8 to 10 parts of carbon black, 20 to 22 parts of silicone rubber compounds, 4 to 6 parts of nanometer SiO<2>, 6 to 8 parts of nanometer Al<2>O<3> and a proper amount of deionized water. The starch, the dextran, the nano calcium carbonate, the ammonium bicarbonate solution, the positively charged polylysine and the hydroxymethyl cellulose are used as main materials; the ferrofluid, the polyion liquid, the silicone rubber compounds, the nanometer SiO<2>, the nanometer Al<2>O<3> and the amidated low methoxyl pectin are added to prepare the force sensitive colloidal fluid; the environmental stress sensitive type immobilized enzyme porous micro-sphere carrier material is jointly prepared; immobilized enzyme can be effectively adsorbed; and the foundation is laid for a biosensor with high sensitivity.
Description
Technical field
The present invention relates to enzyme immobilization carrier Material Field, specifically relate to a kind of environmental stress responsive type immobilized enzyme porous microsphere solid support material and preparation method.
Background technology
Enzyme immobilization technology can realize being separated of the prolongation of half life of enzyme, the raising of stability, recycling and product and enzyme, and enzyme immobilization technology is for all trades and professions.Meeting and microorganism generation chemical bonding in immobilization process, the physical adsorption of its macropore morphological structure and cut sunken implantation effect etc. by formation carrier in conjunction with microbial immobilized system, both be conducive to the metabolism propagation of institute's fixation of microbe, present again excellent mass-transfer performance.
Along with deepening continuously of research, the enzyme immobilization carrier material of Development of Novel is significant, ambient pressure sensor has highly sensitive because of it, has a wide range of applications in life, but uncommon to the enzyme immobilization material of environmental stress sensitivity, develop the enzyme immobilization carrier of environmental stress sensitivity and then realize the highly sensitive environmental organism sensor based on enzymic activity there are huge potentiality.
Summary of the invention
For the growth requirement of the environmental organism sensor based on enzymic activity, the invention provides a kind of environmental stress responsive type immobilized enzyme porous microsphere solid support material and preparation method.
For achieving the above object, the technical solution used in the present invention is:
A kind of environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises by the raw material of following weight part: starch 22-26, oyster shells 20-30, dextran 1 8-20, nano-calcium carbonate 14-16, ammonium bicarbonate soln 7-9, positively charged polylysine 12-16, ferrofluid 5-7, poly ion liquid 14-18, Walocel MT 20.000PV 3-5, amidated pectins 2-3, carbon black 8-10, silicone rubber compound 20-22, Nano-meter SiO_2
24-6, nanometer Al
2o
36-8, appropriate deionized water.
A preparation method for environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises the following steps:
(1) get oyster shells and put into pulverizer, pulverize, put into high-temperature calcination stove afterwards, calcining 30-40min obtains porous oyster shell powder materials for later use;
(2) by ferrofluid, poly ion liquid, silicone rubber compound, Nano-meter SiO_2
2, nanometer Al
2o
3, amidated pectins mixing, and add gross weight 3-4 water doubly, at 40-60 DEG C, heat high-speed stirring 3-4h to even that pressure-sensitive colloidal solution is for subsequent use;
(3) by starch, dextran, nano-calcium carbonate, ammonium bicarbonate soln, positively charged polylysine, Walocel MT 20.000PV mixing, and add gross weight 3-4 water doubly, adopt the ultrasonic assistant concussion process 5-7h of 200W, obtain base fluid for subsequent use;
(4) by porous oyster shell powder material, carbon black, pressure-sensitive colloidal solution, base fluid mix and blend 4-6h extremely evenly, adopt Freeze Drying Technique dry afterwards, put into muffle furnace high temperature 250 DEG C calcining 3-5h, obtain pressure-sensitive porous microsphere.
Beneficial effect of the present invention:
The present invention adopts starch, dextran, nano-calcium carbonate, ammonium bicarbonate soln, positively charged polylysine, Walocel MT 20.000PV to cook major ingredient, adds ferrofluid, poly ion liquid, silicone rubber compound, Nano-meter SiO_2
2, nanometer Al
2o
3, the power responsive type colloidal solution prepared of amidated pectins, jointly prepare environment sensitive type immobilized enzyme porous microsphere solid support material, can active adsorption immobilized enzyme, for highly sensitive biosensor lays the foundation.
Embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is further described.
Embodiment 1:
A kind of environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises by the raw material of following weight part: starch 22, oyster shells 20, dextran 18, nano-calcium carbonate 14, ammonium bicarbonate soln 7, positively charged polylysine 12, ferrofluid 7, poly ion liquid 18, Walocel MT 20.000PV 3, amidated pectins 3, carbon black 8, silicone rubber compound 22, Nano-meter SiO_2
26, nanometer Al
2o
38, appropriate deionized water.
A preparation method for environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises the following steps:
(1) get oyster shells and put into pulverizer, pulverize, put into high-temperature calcination stove afterwards, calcining 35min obtains porous oyster shell powder materials for later use;
(2) by ferrofluid, poly ion liquid, silicone rubber compound, Nano-meter SiO_2
2, nanometer Al
2o
3, amidated pectins mixing, and add the water of gross weight 3.5 times, at 50 DEG C, heat high-speed stirring 3.5h to even that pressure-sensitive colloidal solution is for subsequent use;
(3) by starch, dextran, nano-calcium carbonate, ammonium bicarbonate soln, positively charged polylysine, Walocel MT 20.000PV mixing, and add the water of gross weight 3.5 times, adopt the ultrasonic assistant concussion process 6h of 200W, obtain base fluid for subsequent use;
(4) by porous oyster shell powder material, carbon black, pressure-sensitive colloidal solution, base fluid mix and blend 5h extremely evenly, adopt Freeze Drying Technique dry afterwards, put into muffle furnace high temperature 250 DEG C calcining 4h, obtain pressure-sensitive porous microsphere.
Embodiment 2:
A kind of environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises by the raw material of following weight part: starch 24, oyster shells 25, dextran 19, nano-calcium carbonate 15, ammonium bicarbonate soln 8, positively charged polylysine 14, ferrofluid 6, poly ion liquid 16, Walocel MT 20.000PV 4, amidated pectins 2.5, carbon black 9, silicone rubber compound 21, Nano-meter SiO_2
25, nanometer Al
2o
37, appropriate deionized water.
A preparation method for environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises the following steps:
(1) get oyster shells and put into pulverizer, pulverize, put into high-temperature calcination stove afterwards, calcining 35min obtains porous oyster shell powder materials for later use;
(2) by ferrofluid, poly ion liquid, silicone rubber compound, Nano-meter SiO_2
2, nanometer Al
2o
3, amidated pectins mixing, and add the water of gross weight 3.5 times, at 50 DEG C, heat high-speed stirring 3.5h to even that pressure-sensitive colloidal solution is for subsequent use;
(3) by starch, dextran, nano-calcium carbonate, ammonium bicarbonate soln, positively charged polylysine, Walocel MT 20.000PV mixing, and add the water of gross weight 3.5 times, adopt the ultrasonic assistant concussion process 6h of 200W, obtain base fluid for subsequent use;
(4) by porous oyster shell powder material, carbon black, pressure-sensitive colloidal solution, base fluid mix and blend 5h extremely evenly, adopt Freeze Drying Technique dry afterwards, put into muffle furnace high temperature 250 DEG C calcining 4h, obtain pressure-sensitive porous microsphere.
Embodiment 3:
A kind of environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises by the raw material of following weight part: starch 26, oyster shells 30, Dextran 20, nano-calcium carbonate 16, ammonium bicarbonate soln 9, positively charged polylysine 6, ferrofluid 5, poly ion liquid 14, Walocel MT 20.000PV 5, amidated pectins 2, carbon black 10, silicone rubber compound 20, Nano-meter SiO_2
24, nanometer Al
2o
36, appropriate deionized water.
A preparation method for environmental stress responsive type immobilized enzyme porous microsphere solid support material, comprises the following steps:
(1) get oyster shells and put into pulverizer, pulverize, put into high-temperature calcination stove afterwards, calcining 35min obtains porous oyster shell powder materials for later use;
(2) by ferrofluid, poly ion liquid, silicone rubber compound, Nano-meter SiO_2
2, nanometer Al
2o
3, amidated pectins mixing, and add the water of gross weight 3.5 times, at 50 DEG C, heat high-speed stirring 3.5h to even that pressure-sensitive colloidal solution is for subsequent use;
(3) by starch, dextran, nano-calcium carbonate, ammonium bicarbonate soln, positively charged polylysine, Walocel MT 20.000PV mixing, and add the water of gross weight 3.5 times, adopt the ultrasonic assistant concussion process 6h of 200W, obtain base fluid for subsequent use;
(4) by porous oyster shell powder material, carbon black, pressure-sensitive colloidal solution, base fluid mix and blend 5h extremely evenly, adopt Freeze Drying Technique dry afterwards, put into muffle furnace high temperature 250 DEG C calcining 4h, obtain pressure-sensitive porous microsphere.
The Specifeca tion speeification test result of enzyme immobilization many balls poromerics that above-described embodiment 1-3 obtains is as shown in table 1.
The Specifeca tion speeification of enzyme immobilization many balls poromerics that table 1 embodiment 1-3 obtains
As seen from the above table, enzyme immobilization many balls poromerics aperture of the present invention has good adsorption effect and recycles effect when 14-19um, have good pressure sensitivity simultaneously, have certain meaning to the exploitation of presser sensor biosensor.
Claims (2)
1. an environmental stress responsive type immobilized enzyme porous microsphere solid support material, it is characterized in that, comprise by the raw material of following weight part: starch 22-26, oyster shells 20-30, dextran 1 8-20, nano-calcium carbonate 14-16, ammonium bicarbonate soln 7-9, positively charged polylysine 12-16, ferrofluid 5-7, poly ion liquid 14-18, Walocel MT 20.000PV 3-5, amidated pectins 2-3, carbon black 8-10, silicone rubber compound 20-22, Nano-meter SiO_2
24-6, nanometer Al
2o
36-8, appropriate deionized water.
2. a preparation method for environmental stress responsive type immobilized enzyme porous microsphere solid support material as claimed in claim 1, is characterized in that, comprise the following steps:
(1) get oyster shells and put into pulverizer, pulverize, put into high-temperature calcination stove afterwards, calcining 30-40min obtains porous oyster shell powder materials for later use;
(2) by ferrofluid, poly ion liquid, silicone rubber compound, Nano-meter SiO_2
2, nanometer Al
2o
3, amidated pectins mixing, and add gross weight 3-4 water doubly, at 40-60 DEG C, heat high-speed stirring 3-4h to even that pressure-sensitive colloidal solution is for subsequent use;
(3) by starch, dextran, nano-calcium carbonate, ammonium bicarbonate soln, positively charged polylysine, Walocel MT 20.000PV mixing, and add gross weight 3-4 water doubly, adopt the ultrasonic assistant concussion process 5-7h of 200W, obtain base fluid for subsequent use;
(4) by porous oyster shell powder material, carbon black, pressure-sensitive colloidal solution, base fluid mix and blend 4-6h extremely evenly, adopt Freeze Drying Technique dry afterwards, put into muffle furnace high temperature 250 DEG C calcining 3-5h, obtain pressure-sensitive porous microsphere.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106732436A (en) * | 2017-02-15 | 2017-05-31 | 西华师范大学 | A kind of magnetic starch compound adsorbent preparation method and application |
| CN106946382A (en) * | 2017-05-18 | 2017-07-14 | 付主枝 | A kind of high-efficient environment treating method of heavy metal chromium ion waste water |
| CN113025606A (en) * | 2021-02-25 | 2021-06-25 | 浙江工业大学 | Microbial capsule for tailing restoration and trinity tailing in-situ restoration method |
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2015
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| CN106946382A (en) * | 2017-05-18 | 2017-07-14 | 付主枝 | A kind of high-efficient environment treating method of heavy metal chromium ion waste water |
| CN113025606A (en) * | 2021-02-25 | 2021-06-25 | 浙江工业大学 | Microbial capsule for tailing restoration and trinity tailing in-situ restoration method |
| CN113025606B (en) * | 2021-02-25 | 2023-09-01 | 浙江工业大学 | Microbial capsule for tailing remediation and trinity tailing in-situ remediation method |
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