CN105076127A - Flower staining method of low-concentration 2,4-D gel - Google Patents

Abstract

The invention discloses a flower staining method of low-concentration 2, 4-D gel and belongs to the technical field of plant biology. The flower staining method includes: preparing a 2, 4-D mother solution of 1mg/mL; diluting the mother solution to be a low-concentration 2, 4-D working solution of 10mg/L; using the working solution to prepare a low-concentration 2, 4-D gel blocking solution containing 1% sodium carboxymethylcellulose and 8% sodium alginate; soaking flowers which blossom after 1-2 days in the gel blocking solution for 2-3 seconds, and then taking the flowers out.

Description

A kind of low concentration 2,4-D gel be stained with colored method

Technical field

A kind of low concentration 2,4-D gel be stained with colored method, belong to plant biotechnology field.

Background technology

Being stained with flower is one of important channel of improving plant percentage of fertile fruit, and the method has the plurality of advantages such as cost is low, instant effect, the cycle is short, percentage of fertile fruit is high.Usually can use dissimilar hormone when being stained with colored, as 2,4-D, IBA, NAA, 2,4-D, GA ₃, 6-BA, KT, TDZ, TIBA, ethrel, B ₉, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant body or in vitro significantly Accelerate bloom, improve and bear fruit, agricultural production plays important purposes.But, under existence conditions, be stained with hormone working solution flower fair to cause moisture or nutriment loss due to long high temperature, extremely easily lead to the failure.Therefore, a kind of fast and convenient method is needed badly for improving the one-tenth flower, fruit-setting rate etc. of plant.

Summary of the invention

A kind of low concentration 2,4-D gel be stained with colored method, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with flower, concrete steps operation is as follows:

1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition;

be stained with flower: be soaked in by the flower that 1-2 days open afterwards in above-mentioned hormone gel confining liquid and take out after 2-3s.

The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.

embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description.

Embodiment

A kind of low concentration 2,4-D gel be stained with colored method, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with flower, concrete steps operation is as follows:

1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition;

be stained with flower: the capsicum flower opened 1-2 days is afterwards soaked in above-mentioned hormone gel confining liquid and takes out after 2-3s.

Claims (1)

1. low concentration 2, a 4-D gel be stained with colored method, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with flower, concrete steps operation as follows:

1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition;

be stained with flower: be soaked in by the flower that 1-2 days open afterwards in above-mentioned hormone gel confining liquid and take out after 2-3s.