CN105067556A - Optical device and optical method for quickly detecting biological liquid sample - Google Patents
Optical device and optical method for quickly detecting biological liquid sample Download PDFInfo
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Abstract
The invention discloses an optical device and optical method for quickly detecting a biological liquid sample. The optical device for quickly detecting the biological liquid sample comprises a mode locking Ti sapphire femtosecond laser, a time delay device, gallium arsenide crystals, zinc telluride crystals, a high-speed photoelectric probe, a 780 nm beam splitter, a first 780 nm total reflective mirror, a second 780 nm total reflective mirror, a first focusing lens, a second focusing lens, a first paraboloidal mirror, a second paraboloidal mirror, terahertz spectrum and a sample groove, wherein the time delay device comprises the first 780 nm total reflective mirror and the second 780 nm total reflective mirror perpendicular to each other, and is fixed on a motorized translation stage.
Description
Technical field
The invention belongs to food liquid safety detection, blood sample checkout equipment technical field, particularly relate to a kind of optical devices and optical means of quick detection biological liquid sample.
Background technology
Sample analysis technology refers to as assess sample, carries out analyzing the techniques and methods detected to its special component and content.Sample has multiple sorting technique, by Form division, can be divided into gaseous sample, liquid sample, solid sample etc.; Divide by field, production piece, foodstuff samples, biological sample, environmental sample etc. can be divided into; The sample of different shape, sampling, pre-treatment and determination method are also different.Traditional sample detection analytical approach has: molecular spectrum (ultraviolet-visible spectrophotometry, fluorimetry, chemiluminescence etc.); Chromatogram (GC, LC, HPLC, IC etc.); Atomic spectrum (AAS, AFS, AES or ICP-AES etc.); Electrochemical analysis (potentiometric analysis, sensor, coulometry, polarogram, stripping voltammetry etc.); GC, LC, ICP and MS coupling, the coupling techniques such as FI, SI and photometry, chemiluminescence, atomic spectrum.
Laser measuring technology refers to the excellent specific properties such as the precision utilizing laser is high, measurement range is large, detection time is short, contactless, using it as detection exciting light, is equipped with corresponding photovalve to realize the mensuration inspection of physical quantity or chemical attribute.By using laser measuring technology, the measurement of physical quantity, as displacement, temperature, pressure, speed, refractive index etc., no longer need independent measurement, but whole Physical Quantity Field carries out contactless measurement together.Except the detection of physical quantity, utilize the interaction of light and material, analysis means as new in rotation effect, fluorescent effect, selective absorbing effect etc., laser measuring technology starts to obtain new application at the detection field of matter chemistry attribute.Due to laser detection material have highly sensitive, accuracy good, the feature that analysis speed is fast, opened up brand-new application prospect to sample detection field.
In order to strengthen the detectability of the detectability, particularly environment of society to sample and water sample, in the urgent need to developing a kind of simple and practical, economical and practical and the liquid sample detecting instrument that function is numerous and method.Obviously, the chemical measurement analysis of traditional laboratory a small amount of sample and quality information acquisition methods cannot meet the needs of modernization development.Therefore, explore a kind of new liquid sample detection technique and method, be of great practical significance and using value, be also the inevitable requirement of production development simultaneously.
Summary of the invention
The present invention mainly solves the technical matters existing for above-mentioned prior art, a kind of optical devices and optical means of quick detection biological liquid sample are provided, by spectral informations such as optical instrument extraction time delay, refractive index, absorption coefficient and absorption peaks, utilize these parameter informations to analyze composition and the content of biological liquid sample, thus effectively identify the quality of sample.
Above-mentioned technical matters of the present invention is mainly solved by following technical proposals: a kind of optical devices of quick detection biological liquid sample and optical means, and the optical devices of described quick detection biological liquid sample comprise locked mode titanium sapphire femto-second laser, time delay device, gallium arsenide, zinc telluridse crystal, high speed optoelectronic probe, 780nm beam splitter, a 780nm total reflective mirror, the 2nd 780nm total reflective mirror, the first condenser lens, the second condenser lens, the first paraboloidal mirror, the second paraboloidal mirror, Terahertz wave spectrum, sample cell; Described time delay device by angle each other the 780nm total reflective mirror at right angle and the 2nd 780nm total reflective mirror form, described time delay device is fixed on motorized precision translation stage; Described locked mode titanium sapphire femto-second laser can launch outgoing laser beam, described outgoing laser beam is divided into reference beam and detecting light beam through beam splitter, described detecting light beam converges on wavelength conversion crystal through the second condenser lens, and described detecting light beam converts the millimicron wave spectrum needed for specific sample to after wavelength conversion crystal; Described second paraboloidal mirror arranges manhole, described manhole be convenient for reference light beam through the second paraboloidal mirror and millimicron wave spectrum close restraint after jointly converge on optoelectronic induction crystal; Described motorized precision translation stage is arranged motorized precision translation stage control line, described motorized precision translation stage control line and computer, signalization transmission line on described computer, described signal transmssion line is popped one's head in high speed optoelectronic and is connected.
The optical means of described quick detection biological liquid sample is as follows:
(1) first, multiple laser measuring technology is applied to sample analysis field; According to different sample characteristics of for example, select different laser acquisition wavelength, such as infrared band can effectively differentiate organic characteristic absorption peak, this is because infrared laser is consistent with the absorption of the sum of fundamental frequencies that the hydric group (C-H, N-H) in organic molecule vibrates and frequency multiplication at different levels, thus obtain the eigen vibration information of organic molecule hydric group in sample.Different detection wavelength is by selecting different wavelength conversion crystal to realize, such as bbo crystal can realize near-infrared wavelength and export, BIBO crystal can realize middle infrared wavelength and export, and gallium selenide crystal can realize far infrared wavelength and export, and gallium arsenide can realize Terahertz wave spectrum and export.The different bandwidth of output wavelength, different-energy, different polarization and the difference ellipse degree of bias also can be realized by tuning crystal angle.
(2) then, the sample spectra information collected also needs to carry out analyzing and processing, and Pretreated spectra algorithm comprises Savitzky-GolaySmoothing smoothing method (SGS), standard normalization (SNV), Wavelet Denoising Method etc.By adopting multiple spectrum variable Optimization Selection Algorithm and combinational algorithm thereof, full wave spectral variables is selected and characteristic spectrum signal extraction, spectrum useful information distribution per sample simultaneously, garbage distributes, redundant information distributes, noise profile etc., extract the spectral band of corresponding heterogeneity information, reject the impact of disturbing factor.
(3) last, also needing the standard model information bank setting up known composition and content, by analyzing the information of contrast testing sample and standard model, thus identifying composition and the content of testing sample.Sample message, mainly from the result such as shape analysis, intensive analysis, position analysis of spectral signal, differentiates composition and the content of testing sample by time delay, absorption coefficient, the parameter information such as absorption position and refractive index extracting them.
The beneficial effect that the present invention has: by spectral informations such as optical instrument extraction time delay, refractive index, absorption coefficient and absorption peaks, utilize these parameter informations to analyze composition and the content of biological liquid sample, thus effectively identify the quality of sample; The present invention can realize the integrated informations such as quick, accurate, in enormous quantities, contactless, non-destructively to obtain liquid sample continuously composition and content; Compared with traditional chemical detection analytical approach, this specimen inspection system based on multiple laser measuring technology has many outstanding advantages:
(1) laser measuring technology belongs to non-contact measuring technology, compared with contact measurement method, have that restriction is less, efficiency is higher, the not easily advantage such as lesioned sample form, survivable sample properties, it is a kind of potential quick, easy, economic sample detection methods, detection efficiency can be improved, reduce testing cost, enlarge measurement range.
(2) adopt multiple laser measuring technology to detect the chemical attribute of liquid sample, analysis means comprises the shape analysis, intensive analysis, position analysis etc. of spectrum, and sensing range comprises the mensuration of the compositions such as inorganics, organism, microorganism and content.Traditional chemical reagent method, detection material is single, and multi-functional giant chemical detecting instrument is expensive, should not popularize.Therefore, laser measuring technology is adopted to compensate for all drawbacks and the deficiency of classic method.
The present invention will contribute to the intellectuality of sample detection means and rapid, and the standard science realizing liquid sample detects, and advances the science of China's sample detection industry to develop in a healthy way.The instrument and equipment of exploitation will have broad application prospects and economic worth.With regard to domestic present case, seldom have application and development in this respect, but market outlook are very huge, except the sample detection demand of enterprise self, only the Minister of the General Administration for Quality Supervision of national administrative department at county level just has much for the detection demand of environment liquid sample.
Accompanying drawing explanation
Fig. 1 is optical devices schematic diagram of the present invention.
Fig. 2 is optical means Technology Roadmap of the present invention.
Fig. 3 is a concrete device schematic diagram implementing example of the present invention.
Fig. 4 is a concrete Technology Roadmap implementing example of the present invention.
Fig. 5 is for the present invention specifically implements time-domain spectroscopy figure and the frequency domain spectra figure of example.
In figure: 1, locked mode titanium sapphire femto-second laser; 2, time delay device; 3, gallium arsenide; 4, zinc telluridse crystal; 5, high speed optoelectronic probe; 6, outgoing laser beam; 7,780nm beam splitter; 8, a 780nm total reflective mirror; 9, the 2nd 780nm total reflective mirror; 10, the first condenser lens; 11, the second condenser lens; 12, the first paraboloidal mirror; 13, the second paraboloidal mirror; 14, reference beam; 15, detecting light beam; 16, Terahertz wave spectrum; 17, sample cell; 18, computer; 19, motorized precision translation stage control line; 20, signal transmssion line; 21, motorized precision translation stage; 22, manhole.
Embodiment
Below by embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.
Embodiment: a kind of optical devices of quick detection biological liquid sample and optical means, as shown in Fig. 1 ~ Fig. 2, the optical devices of described quick detection biological liquid sample comprise locked mode titanium sapphire femto-second laser, time delay device, gallium arsenide, zinc telluridse crystal, high speed optoelectronic probe, 780nm beam splitter, a 780nm total reflective mirror, the 2nd 780nm total reflective mirror, the first condenser lens, the second condenser lens, the first paraboloidal mirror, the second paraboloidal mirror, Terahertz wave spectrum, sample cell; Described time delay device by angle each other the 780nm total reflective mirror at right angle and the 2nd 780nm total reflective mirror form, described time delay device is fixed on motorized precision translation stage; Described locked mode titanium sapphire femto-second laser can launch outgoing laser beam, described outgoing laser beam is divided into reference beam and detecting light beam through beam splitter, described detecting light beam converges on wavelength conversion crystal through the second condenser lens, and described detecting light beam converts the millimicron wave spectrum needed for specific sample to after wavelength conversion crystal; Described second paraboloidal mirror arranges manhole, described manhole be convenient for reference light beam through the second paraboloidal mirror and millimicron wave spectrum close restraint after jointly converge on optoelectronic induction crystal; Described motorized precision translation stage is arranged motorized precision translation stage control line, described motorized precision translation stage control line and computer, signalization transmission line on described computer, described signal transmssion line is popped one's head in high speed optoelectronic and is connected.
The optical means of described quick detection biological liquid sample is as follows:
(1) first, multiple laser measuring technology is applied to sample analysis field; According to different sample characteristics of for example, select different laser acquisition wavelength, such as infrared band can effectively differentiate organic characteristic absorption peak, this is because infrared laser is consistent with the absorption of the sum of fundamental frequencies that the hydric group (C-H, N-H) in organic molecule vibrates and frequency multiplication at different levels, thus obtain the eigen vibration information of organic molecule hydric group in sample.Different detection wavelength is by selecting different wavelength conversion crystal to realize, such as bbo crystal can realize near-infrared wavelength and export, BIBO crystal can realize middle infrared wavelength and export, and gallium selenide crystal can realize far infrared wavelength and export, and gallium arsenide can realize Terahertz wave spectrum and export.The different bandwidth of output wavelength, different-energy, different polarization and the difference ellipse degree of bias also can be realized by tuning crystal angle.
(2) then, the sample spectra information collected also needs to carry out analyzing and processing, and Pretreated spectra algorithm comprises Savitzky-GolaySmoothing smoothing method (SGS), standard normalization (SNV), Wavelet Denoising Method etc.By adopting multiple spectrum variable Optimization Selection Algorithm and combinational algorithm thereof, full wave spectral variables is selected and characteristic spectrum signal extraction, spectrum useful information distribution per sample simultaneously, garbage distributes, redundant information distributes, noise profile etc., extract the spectral band of corresponding heterogeneity information, reject the impact of disturbing factor.
(3) last, also needing the standard model information bank setting up known composition and content, by analyzing the information of contrast testing sample and standard model, thus identifying composition and the content of testing sample.Sample message, mainly from the result such as shape analysis, intensive analysis, position analysis of spectral signal, differentiates composition and the content of testing sample by time delay, absorption coefficient, the parameter information such as absorption position and refractive index extracting them.
Specific experiment process is as follows: the outgoing laser beam of 780nm femtosecond is divided into reference light and detection light through 780nm beam splitter, and detection light focuses on gallium arsenide through the second condenser lens, and then gives off Terahertz wave spectrum.Terahertz wave spectrum focuses on sample through the first paraboloidal mirror, Terahertz wave spectrum and reference light copolymerization are on the zinc telluridse crystal of electrooptical measuring device, reference light and the time delay detecting light is regulated by time delay device, scan the time domain waveform of terahertz pulse, then signal reaches computer through lock-in amplifier.The technology path of whole experimental program as shown in Figure 4.In addition, in order to avoid water vapor in air equimolecular is to the strong absorption of THz wave, the generation sniffer of THz wave need be sealed in and be full of in the cover of drying nitrogen, ensure that inner relative humidity is less than 5%, temperature controls near 295K.
Application device as shown in Figure 3 and the technology path shown in Fig. 4, the time-domain spectroscopy figure (left side) of this three vegetable oil of the soya-bean oil obtained, rapeseed oil and blending stock before and after high-temperature heating and frequency domain spectra figure (right side) as shown in Figure 5.Can find out from time-domain diagram, relative to before heating after heating, the amplitude of output signal reduces and time delay increases; Can find out from frequency domain figure, the oscillator intensity before and after heating and absorption peak etc. there are differences, and this makes grease composition there occurs change owing to heating; By information such as the shape of analytic signal, intensity and positions, namely can be and effectively differentiate that Various Edible provides foundation.
By spectral informations such as optical instrument extraction time delay, refractive index, absorption coefficient and absorption peaks, utilize these parameter informations to analyze composition and the content of biological liquid sample, thus effectively identify the quality of sample; The present invention can realize the integrated informations such as quick, accurate, in enormous quantities, contactless, non-destructively to obtain liquid sample continuously composition and content; Compared with traditional chemical detection analytical approach, this specimen inspection system based on multiple laser measuring technology has many outstanding advantages.
Finally, it should be pointed out that above embodiment is only the more representational example of the present invention.Obviously, the invention is not restricted to above-described embodiment, many distortion can also be had.Every above embodiment is done according to technical spirit of the present invention any simple modification, equivalent variations and modification, all should think and belong to protection scope of the present invention.
Claims (2)
1. detect optical devices and the optical means of biological liquid sample fast, it is characterized in that the optical devices of described quick detection biological liquid sample comprise locked mode titanium sapphire femto-second laser, time delay device, gallium arsenide, zinc telluridse crystal, high speed optoelectronic probe, 780nm beam splitter, a 780nm total reflective mirror, the 2nd 780nm total reflective mirror, the first condenser lens, the second condenser lens, the first paraboloidal mirror, the second paraboloidal mirror, Terahertz wave spectrum, sample cell; Described time delay device by angle each other the 780nm total reflective mirror at right angle and the 2nd 780nm total reflective mirror form, described time delay device is fixed on motorized precision translation stage; Described locked mode titanium sapphire femto-second laser can launch outgoing laser beam, described outgoing laser beam is divided into reference beam and detecting light beam through beam splitter, described detecting light beam converges on wavelength conversion crystal through the second condenser lens, and described detecting light beam converts the millimicron wave spectrum needed for specific sample to after wavelength conversion crystal; Described second paraboloidal mirror arranges manhole, described manhole be convenient for reference light beam through the second paraboloidal mirror and millimicron wave spectrum close restraint after jointly converge on optoelectronic induction crystal; Described motorized precision translation stage is arranged motorized precision translation stage control line, described motorized precision translation stage control line and computer, signalization transmission line on described computer, described signal transmssion line is popped one's head in high speed optoelectronic and is connected.
2. a kind of optical devices of quick detection biological liquid sample and optical means according to claim 1, is characterized in that the optical means of described quick detection biological liquid sample is as follows:
(1) first, multiple laser measuring technology is applied to sample analysis field; According to different sample characteristics of for example, select different laser acquisition wavelength, such as infrared band can effectively differentiate organic characteristic absorption peak, this is because infrared laser is consistent with the absorption of the sum of fundamental frequencies that the hydric group (C-H, N-H) in organic molecule vibrates and frequency multiplication at different levels, thus obtain the eigen vibration information of organic molecule hydric group in sample.Different detection wavelength is by selecting different wavelength conversion crystal to realize, such as bbo crystal can realize near-infrared wavelength and export, BIBO crystal can realize middle infrared wavelength and export, and gallium selenide crystal can realize far infrared wavelength and export, and gallium arsenide can realize Terahertz wave spectrum and export.The different bandwidth of output wavelength, different-energy, different polarization and the difference ellipse degree of bias also can be realized by tuning crystal angle.
(2) then, the sample spectra information collected also needs to carry out analyzing and processing, and Pretreated spectra algorithm comprises Savitzky-GolaySmoothing smoothing method (SGS), standard normalization (SNV), Wavelet Denoising Method etc.By adopting multiple spectrum variable Optimization Selection Algorithm and combinational algorithm thereof, full wave spectral variables is selected and characteristic spectrum signal extraction, spectrum useful information distribution per sample simultaneously, garbage distributes, redundant information distributes, noise profile etc., extract the spectral band of corresponding heterogeneity information, reject the impact of disturbing factor.
(3) last, also needing the standard model information bank setting up known composition and content, by analyzing the information of contrast testing sample and standard model, thus identifying composition and the content of testing sample.Sample message, mainly from the result such as shape analysis, intensive analysis, position analysis of spectral signal, differentiates composition and the content of testing sample by time delay, absorption coefficient, the parameter information such as absorption position and refractive index extracting them.
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Application publication date: 20151118 |