CN105058546B - A kind of enzymatic treatment environmental protection deal board - Google Patents
A kind of enzymatic treatment environmental protection deal board Download PDFInfo
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- CN105058546B CN105058546B CN201510478917.7A CN201510478917A CN105058546B CN 105058546 B CN105058546 B CN 105058546B CN 201510478917 A CN201510478917 A CN 201510478917A CN 105058546 B CN105058546 B CN 105058546B
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Abstract
The invention discloses providing a kind of laccase, zytase, carbohydrase to cooperate with processing pine cellulosic to prepare the technique of deal board, the technique can significantly reduce the usage amount of enzyme, and obtain the fiberboard of internal bond strength higher.Its step is as follows：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 3.5 4.5 to adjust to desired pH value, leads to people's oxygen, reacts 50 70min at 28 30 DEG C, taking-up drains；Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while acting on to rock down and make slab smooth by liquid, and the fibre mat after draining first is cold-pressed water squeezing, then hot pressing.
Description
Technical field
The present invention relates to a kind of production technology of enzymatic treatment environmental protection deal board, belong to wood processing field.
Background technology
With the fast development of economic society, the demand to wildwood timber is in increase trend rapidly both at home and abroad.In recent years
Come, with the raising of countries in the world environmental consciousness, retrenchment policy has been carried out to timber export, further limit natural timber
Outlet, is further exacerbated by the nervous situation of timber demand.Develop the alternative material of wildwood timber, be to alleviate current natural forest
The important channel of material supply and demand anxiety contradiction, and the inevitable requirement built a conservation-minded society.Fast-growing, high-yield woods is that China is non-at present
Often abundant forest tree resource, China are successively proposed six big key project of forestry since last century Mo, wherein it is speed to have four
Raw high yield Timber stands construction project, is related to national 18 provinces, builds nearly 40,000,000 hectares of total scale, plan by 2015 or so to build
Vertical nearly 600,000,000 mu of fast-growing and high-yield timber forest base.
On the cultural history of furniture, the intention of the founder Nordics of pine furniture is performed meritorious deeds never to be obliterated, they, which change, rotten is
Script, is become high-quality plate, so as to make pine simple with it by mystery by the crowded quick growing species of trees of excess resin through tread flaking drying
Simple and unadorned texture, lifelike texture, pure beautiful color and luster, domestic environment is decorateeed plain, it is pure it is quiet, incorporate with it is natural
Harmony with peaceful, thus pine furniture is set to environmentally friendly furniture by humanity portion of the United Nations.Pine furniture visually first take by force by " color "
People.Indifferent to fame or gain soft pine true qualities " natural beauty difficulty is given up as hopeless " allow the low eyebrow of other seeds to bow one's head.It is not added with the pine true qualities man of carve
Tool is natural views, is nature landscape, smart spacious texture, fine and smooth lines, hard and soft and Ji.Pine is that a kind of confierophyte is (normal
The confierophyte seen has pine, China fir, cypress), it has rosin taste, color is yellowish, furuncle scar is more, appearance fast to atmospheric temperature reaction
Easily swell, the characteristic such as extremely difficult natural air drying, therefore need to be through artificial treatment, such as drying, degreasing removes organic compound, bleaches unified tree
Color, neutralizes tree property, is allowed to be unlikely to deform.
《Forest-science》The phase " enzyme activation treatment conditions and its influence pre-test to pine cellulosic glue performance " of volume 40 the 4th is public
Opened a kind of method with laccase treatment pinus khasys fiber and pressboard, the internal bond strength of plate (or combined between slab strong
Degree) the plate internal bond strength that is pressed into the fiber after laccase treatment of measurement result proof up to more than 1.0MPa, is significantly higher than
Water process fiber pressed sheet intensity.Enzyme liquid after inactivation is equivalent with water, illustrates other nutritional ingredients and auxiliary agent in enzyme liquid
Contribution to realizing timber Auto adhesion can be ignored, to realizing mainly laccase that timber Auto adhesion works in itself.And
And the optimal parameter measured is:Enzyme dosage:12-15U/g fibers；Concentration of substrate:5%；PH value:3.0 1 4.0；Enzymatic reaction temperature
Degree:(26 scholar 4) `C；Enzyme processing time:2h；Hot pressing temperature:More than 140 DEG C.Using above-mentioned enzymatic treatment and sheeting process parameter, pressure
300mmx300mmx8mm fiberboards are made, the density for obtaining plate is 1.1575g/cm3, internal bond strength measurement result is
1.536MPa.
The present invention is intended to provide a kind of laccase, zytase, carbohydrase collaboration processing pine cellulosic prepare the work of deal board
Skill, the technique can significantly reduce the usage amount of enzyme, and obtain the fiberboard of internal bond strength higher.
The content of the invention
Based on technical problem existing for background technology, the present invention for background technology there are the problem of, there is provided a kind of laccase,
Zytase, carbohydrase collaboration processing pine cellulosic prepare the technique of deal board, and the technique can significantly reduce the usage amount of enzyme,
And obtain the fiberboard of internal bond strength higher.
The purpose of the present invention is achieved through the following technical solutions：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 3.5- to adjust to desired pH value
4.5, lead to people's oxygen, react 50-70min at 28-30 DEG C, taking-up drains；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
Preferably, the design parameter of the enzymatic treatment is：Laccase dosage:5-6U/g fibers, zytase dosage:0.5-
0.8U/g fibers, be saccharified enzyme dosage:1-1.5U/g fibrous substrates concentration:5%；
Preferably, the hot pressing temperature:120-150℃；
Preferably, the pressed fiber board 400mmx400mmx9mm.
Generally, it is considered that the effect of laccase and lignin has two kinds of possible results.First, lignin is farthest degraded, this
On the one hand possible application prospect is mainly association with pulp bleaching, and papermaking wastewater is handled；Another kind is that the phenol of lignin is oxidized to through base
Polymer is generated after phenol oxygen-cent red radical, possible application prospect is to realize that timber Auto adhesion and exploitation one unsaturated monomer of lignin are total to
Poly- one enzyme of chemistry triggers system.In short, laccase can increase fiber finer cell wall Auto adhesion power.Present inventors have unexpectedly found that paint
Enzyme, zytase, carbohydrase can cooperate with processing pine cellulosic, and effect is more preferable, and dosage more saves, and cost is lower, this is the present invention
Great discovery.
The invention has the beneficial effects that：
1. present invention process is environmentally friendly, without harmful substance；
2. the deal board bond strength that the present invention is prepared is high；
3. the present invention carries out coordinative role by several enzymes, the dosage and cost of enzyme are significantly reduced.
Embodiment
Embodiment 1：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 3.5 to adjust to desired pH value,
Lead to people's oxygen, react 50min at 30 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Laccase dosage:6U/g fibers, zytase dosage:0.5U/g fibers, saccharification
Enzyme dosage:1.5U/g, fibrous substrates concentration:5%；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
The hot pressing temperature:120℃；
Hot pressing mode is double-deck slab hot pressing, and using the upper cold lower technique of heat, the size of the pressed fiber board is：
400mmx400mmx9mm。
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 2：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 4.5 to adjust to desired pH value,
Lead to people's oxygen, react 70min at 28 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Laccase dosage:5U/g fibers, zytase dosage:0.8U/g fibers, saccharification
Enzyme dosage:1U/g, fibrous substrates concentration:5%；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
The hot pressing temperature:150℃；
Hot pressing mode is double-deck slab hot pressing, and using the upper cold lower technique of heat, the size of the pressed fiber board is：
400mmx400mmx9mm。
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 3：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 3.9 to adjust to desired pH value,
Lead to people's oxygen, react 60min at 29 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Laccase dosage:6U/g fibers, zytase dosage:0.6U/g fibers, saccharification
Enzyme dosage:1.4U/g, fibrous substrates concentration:5%；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
The hot pressing temperature:140℃；
Hot pressing mode is double-deck slab hot pressing, and using the upper cold lower technique of heat, the size of the pressed fiber board is：
400mmx400mmx9mm。
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 4：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 4.4 to adjust to desired pH value,
Lead to people's oxygen, react 55min at 30 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Laccase dosage:5U/g fibers, zytase dosage:0.7U/g fibers, saccharification
Enzyme dosage:1.1U/g, fibrous substrates concentration:5%；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
The hot pressing temperature:130℃；
Hot pressing mode is double-deck slab hot pressing, and using the upper cold lower technique of heat, the size of the pressed fiber board is：
400mmx400mmx9mm。
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 5：
Only 1 is tested as a comparison with laccase treatment：
Comparative example 1：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 4.0 to adjust to desired pH value,
Lead to people's oxygen, react 65min at 28 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Enzyme dosage:14U/g fibers；Concentration of substrate:5%；PH value:4.0；Enzymatic is anti-
Answer temperature:30℃；Enzyme processing time:2h；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
Hot pressing temperature:140 DEG C, suppress 300mmx300mmx8mm fiberboards；
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 6：
Zytase is only used, saccharification enzymatic treatment tests 2 as a comparison：
Comparative example 2：
A kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 4.3 to adjust to desired pH value,
Lead to people's oxygen, react 68min at 29 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Zytase dosage:0.7U/g fibers, be saccharified enzyme dosage:1.2U/g, fiber
Concentration of substrate:5%；
(3) it is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while by the effect of liquid
Make slab smooth in the case where rocking, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
The hot pressing temperature:145℃；
Hot pressing mode is double-deck slab hot pressing, and using the upper cold lower technique of heat, the size of the pressed fiber board is：
400mmx400mmx9mm。
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 7：
3 are tested as a comparison without using processing：
Comparative example 3：
A kind of production method of deal board, its step are as follows：
(1) raw material is prepared：Prepare pine cellulosic；
(2) control treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, adjusting to desired pH value is
3.7, lead to people's oxygen, react 58min at 30 DEG C, taking-up drains；
The design parameter of the enzymatic treatment is：Enzyme solutions, fibrous substrates concentration are replaced with water:5%；
(3) it is hot-forming：Fiber pulp after control treatment is poured into shaping frame, moisture filters out, while by the work of liquid
Make slab smooth used in rocking down, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
The hot pressing temperature:125℃；
Hot pressing mode is double-deck slab hot pressing, and using the upper cold lower technique of heat, the size of the pressed fiber board is：
400mmx400mmx9mm。
By 4.8 progress doubling plates of GB/T 17657-1999 " test methods of evaluating the properties of wood-based panels and surface decorated wood-based panels "
Base pressed sheet, measured intensity level are actually the bond strength between two slabs.
Embodiment 1-7 testing results are recorded, the results are shown in Table 1：
Table 1
| Density (g/cm3) | Internal bond strength (MPa) | |
| Embodiment 1 | 1.58 | 1.87 |
| Embodiment 2 | 1.58 | 1.92 |
| Embodiment 3 | 1.58 | 1.85 |
| Embodiment 4 | 1.58 | 1.79 |
| Embodiment 5 | 1.58 | 1.52 |
| Embodiment 6 | 1.58 | 0.98 |
| Embodiment 7 | 1.58 | 0.90 |
It can be seen from the above that deal board internal bond strength produced by the present invention is very high, laccase, zytase, saccharification enzymatic treatment pine
There is significant synergistic effect, its effect is more preferable, and dosage more saves, and cost is lower, this is the great discovery of the present invention during wood-fibred.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (1)
1. a kind of production method of enzymatic treatment environmental protection deal board, its step are as follows：
（1）Prepare raw material：Prepare pine cellulosic；
（2）Enzymatic treatment：Pine cellulosic is uniformly mixed in a reservoir with water, enzyme liquid, it is 3.5-4.5 to adjust to desired pH value,
Lead to people's oxygen, react 50-70min at 28-30 DEG C, taking-up drains；
（3）It is hot-forming：Fiber pulp after enzymatic treatment is poured into shaping frame, moisture filters out, while acts on rolling by liquid
Make slab smooth under dynamic, the fibre mat after draining first is cold-pressed water squeezing, then hot pressing；
Wherein,
The design parameter of the enzymatic treatment is：Laccase dosage:5-6U/g fibers, zytase dosage:0.5-0.8U/g fibers, sugar
Change enzyme dosage:1-1.5U/g fibers, fibrous substrates concentration:5%；
The temperature of the hot pressing:120-150℃；
The fiberboard of the hot pressing is 400mmx400mmx9mm.
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| CN107443533A (en) * | 2016-06-01 | 2017-12-08 | 鲁玺 | The manufacture method of environmentally friendly dry method Thermo-bonding fibres plate |
| CN107127861A (en) * | 2017-06-04 | 2017-09-05 | 向德超 | A kind of preparation method of moisture retardant particieboard |
| CN107160530A (en) * | 2017-07-15 | 2017-09-15 | 芜湖乾凯材料科技有限公司 | High strength fiberboard and preparation method thereof |
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| FI970158A (en) * | 1997-01-14 | 1998-07-15 | Neste Oy | New fiberboard adhesive |
| FI120045B (en) * | 2005-12-22 | 2009-06-15 | Roal Oy | Treatment of cellulose materials and enzymes useful therein |
| BR112013032861A2 (en) * | 2011-07-22 | 2017-01-24 | Novozymes North America Inc | methods for increasing cellulolytic enzyme activity during hydrolysis of cellulosic material, for hydrolyzing a pretreated cellulosic material, for producing a fermentation product, and for fermenting a pretreated cellulosic material |
| US20150376833A1 (en) * | 2012-06-22 | 2015-12-31 | Suganit Systems, Inc | Method and apparatus for treatment of biomass substrates |
| CN103937768A (en) * | 2014-02-14 | 2014-07-23 | 济南诺能生物工程有限公司 | Preparation method of energy-saving papermaking compound enzyme |
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