CN105055544A - Plant extract capable of preventing brain aging - Google Patents
Plant extract capable of preventing brain aging Download PDFInfo
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Abstract
The invention discloses a plant extract capable of preventing brain aging. A preparation method of the extract comprises the following steps: (a) mixing and crushing 80-90 parts of melastoma sanguineum and 10-20 parts of rhubarb in every 100 parts of medicinal materials, carrying out heat reflux extraction with a 60% ethanol solution, mixing extracting solutions, and concentrating until the mixed solution has no alcohol taste so as to obtain an ethanol extraction concentrated solution; (b) diluting the ethanol extraction concentrated solution obtained in the step (a) with water, sequentially extracting with petroleum ether, ethyl acetate and water-saturated n-butyl alcohol, and carrying out vacuum concentration to respectively obtain a petroleum ether extract, an ethyl acetate extract and an n-butyl alcohol extract; (c) dissolving the n-butyl alcohol extract with water, filtering, enriching active ingredients with macroporous resin, flushing 12 column volumes firstly with 8% ethanol for removing large polar ingredients, then eluting 10 column volumes with 70% ethanol, collecting 70% eluent, and carrying out vacuum concentration to obtain the plant extract. The extract has the effect of preventing brain aging and can be developed into medicines for preventing brain aging.
Description
Technical field
The present invention relates to Chinese medicine extract field, be specifically related to the old plant extract of a kind of anti senile and the pharmaceutical preparation containing this extract, it is old that this extract may be used for anti senile.
Background technology
Medical material Radix Melastomatis Sanguinei is leaf or the Herb of Genus Melastoma L. of Melastomataceae Radix Melastomatis Sanguinei, has another name called jackal Canis familiaris L. tongue, red quick-fried Herba Agrimoniae (" conventional Chinese herbal medicine handbook "), jackal tooth youth (" Guangxi book on Chinese herbal medicine a collection of selected materials "), red core pig harvest (" Huiyang Chinese herbal medicine ").
Former plant Radix Melastomatis Sanguinei (formal name used at school: MelastomasanguineumSims) has another name called sweet ma, opening Fructus Jujubae, for the upright shrub of Melastomataceae (Melastomataceae), leaf to life, ovum shape lanceolar to lanceolar, long 10-14 centimetre, wide 2.5-5 centimetre, the long gradually point in top, basal circular or blunt, has longitudinal vein 5, the outside of belly is light without hair, but in the raw short and thick hair of surface volt; Spend greatly, be born in sheath for 1-3, diameter can reach 7-8 centimetre, aubergine; Calyx pipe range 1-1.5 centimetre, drapes over one's shoulders long and hard bristle, and flower is extensively opened up by base portion, sliver 5-7, and triangle, to triangular shape lanceolar, is short far beyond calyx pipe, has the attached sliver of equal wammel shape; Petal 5-7 sheet, wealthy cochlear, long 3-5 centimetre; Fruit is cup-shaped, and top is wider than base portion, long 1.2-1.6 centimetre, the coarse wool that color of draping a band of red silk over sb.'s shoulders is long and hard.Be distributed in south China, India, the ground such as Malaysia.Radix Melastomatis Sanguinei flower is large and gorgeous, and be good garden potted plant, mellow fruit is fragrant and sweet good to eat, can eat something rare, and also can be used to steep in wine.Root leaf has astringing to arrest bleeding, and help digestion the medical values such as dysentery relieving.
Former plant Radix Melastomatis Sanguinei is large shrub, high 1.5-3 rice; Stem, sprig, petiole, bennet and calyx are all by open and flat long coarse wool, and wool base expands.The hard papery of blade, ovum shape lanceolar is to lanceolar, and top is long gradually sharp or gradually sharp, base portion is blunt or circular, long 8-15 (-22) centimetre, wide 2.5-5 (-8) centimetre, Quan Yuan, base goes out arteries and veins 5, and two sides is hidden in subepidermal strigose, usually only tippy tea end exposes, it is recessed that blade face base goes out arteries and veins, and lateral vein is not obvious, and back side base goes out arteries and veins protuberance, the micro-protuberance of lateral vein, all by thin strigose that base portion expands; The long 1.5-2.5 (-4) centimetre of petiole.Corymb, top is raw, often only spends 1, sometimes 3 (-5) piece; Bract halberd shape, film quality, top is gradually sharp, the back side by short strigose, ridge being close, tool echinid; Bennet is about 5 millimeters, calyx pipe range 1-2 centimetre, diameter 1-2 centimetre, and hair is anti-outward sometimes, sliver 5 (-7), triangle, to triangular shape lanceolar, is about 1.2 centimetres, wide 4 millimeters, slightly short compared with calyx pipe, by strigose on ridge, between sliver, the linear or wire lanceolar lobelet of tool, usually slightly short compared with sliver, petal pink or aubergine, 5 (-7) piece, wide obovate, top is tiltedly slightly biased, top nick, long 3-5 centimetre, wide 2-2.2 centimetre; Stamen elder connective base portion stretches, and end 2 splits, long 1.3 centimetres of flower pesticide, and the connective that filigree comparatively extends is slightly short, and short person's connective does not stretch, long 9 millimeters of flower pesticide, base portion tool 2 tubercle; Ovary half is the next, close by bristle.Fruit cup-shaped is spherical, and placenta meat, by persistent calyx is wrapped; Persistent calyx is close by the long bristle of redness, long 1.5-2.2 centimetre, diameter 1.5-2 centimetre.Flowering fruit bearing stage is almost annual, usually in the 8-10 month.
Summary of the invention
The object of the present invention is to provide a kind of Radix Melastomatis Sanguinei extract, its preparation method and liquid phase analysis method, the pharmaceutical preparation containing this extract and utilize this extract to prepare the purposes of the old medicine for the treatment of anti senile.
Above-mentioned purpose of the present invention is achieved by technical scheme below:
A kind of Radix Melastomatis Sanguinei extract, this extract is prepared by following methods:
A () by weight, every 100 parts of medical materials comprise dry Radix Melastomatis Sanguinei 80 ~ 90 parts and Radix Et Rhizoma Rhei 10 ~ 20 parts, co-grinding, and with 60% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, uses macroporous resin enrichment active component, first remove large polar component with 8% alcohol flushing, 12 column volumes, then use 70% ethanol elution, 10 column volumes, collect 70% eluent, concentrating under reduced pressure and get final product.
Further, macroporous resin described in step (c) is AB-8 type macroporous resin.
In order to be controlled the differences between batches of extract prepared by different batches by the method setting up HPLC finger printing, the liquid phase analysis method of described extract is:
Chromatographic column: AgilentZorbaxSB-C18 post (4.6mm × 250mm, 5 μm);
Mobile phase: A is acetonitrile, and B is 0.4% phosphoric acid solution;
Gradient elution program: 0.01 ~ 5min, A10% → 15%; 5 ~ 15min, A15% → 48%; 15 ~ 25min, A48% → 78%; 25 ~ 40min, A78% → 10%;
Flow rate of mobile phase: 1.0mLmin
-1;
Determined wavelength: 260nm; Column temperature: 30 DEG C; Sample size: 10 μ L.
Pharmaceutical preparation, the described extract containing treatment effective dose and pharmaceutically acceptable carrier.
The application of described extract in the medicine that preparation treatment anti senile is old.
The application of described pharmaceutical preparation in the medicine that preparation treatment anti senile is old.
When extract of the present invention is used as medicine, directly can uses, or use in the form of a pharmaceutical preparation.
Described pharmaceutical preparation contains the Radix Melastomatis Sanguinei extract of the present invention for the treatment of effective dose, and all the other are acceptable on materia medica, nontoxic to humans and animals and pharmaceutically suitable carrier of inertia and/or excipient.
Described pharmaceutically suitable carrier or excipient are that one or more are selected from solid, semisolid and liquid diluent, filler and pharmaceutical preparation adjuvant.Pharmaceutical preparation of the present invention is used with the form of per weight dose.Extract of the present invention is applied to by form that is oral or injection the patient needing treatment.For time oral, tablet, slow releasing tablet, controlled release tablet, capsule, drop pill, micropill, suspensoid, Emulsion, powder or granule, oral liquid etc. can be made into; During for injecting, can be made into aqueous or oily solution, aseptic powder injection, liposome or the Emulsion etc. of sterilizing.
Advantage of the present invention: the present invention by extracting the Radix Melastomatis Sanguinei of drying, remove impurity, enrichment process, the extract with the old function of anti senile can be obtained; Liquid phase analysis method provided by the invention may be used for the finger printing setting up this extract, for controlling the differences between batches of extract prepared by different batches.
Accompanying drawing explanation
Fig. 1 is Radix Melastomatis Sanguinei extractive HPLC chromatograms.
Detailed description of the invention
Further illustrate essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although be explained in detail the present invention with reference to preferred embodiment, those of ordinary skill in the art should be appreciated that and can modify to technical scheme of the present invention or equivalent replacement, and does not depart from essence and the scope of technical solution of the present invention.
Embodiment 1: extract preparation (Radix Melastomatis Sanguinei 85 parts, Radix Et Rhizoma Rhei 15 parts)
Crude drug source: Radix Melastomatis Sanguinei and Radix Et Rhizoma Rhei dry rhizome are purchased from Hui nationality's Chinese Medicinal Materials Markets.
Main agents: food-grade ethanol is purchased from Shanghai Ling Feng chemical reagent company limited; Pharmaceutical grade AB-8 macroporous resin is purchased from sky tunami letter resin company limited; Acetonitrile is HPLC level, is purchased from TEDIA; 85% phosphoric acid is HPLC level, is purchased from TEDIA; Chromatographic grade pure water is heartily pure water.
Preparation method: by the Radix Et Rhizoma Rhei dry rhizome co-grinding of the Radix Melastomatis Sanguinei of 8.5kg drying and 1.5kg, with 60% alcoholic solution circumfluence distillation (25L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution (2L) without alcohol taste; Gained ethanol extraction concentrated solution is diluted with water to 3L, petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) is used to extract successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract 212g respectively; (c) n-butyl alcohol extract 1.5L water dissolution, medical absorbent cotton filters, with AB-8 macroporous resin (2kg, column volume 1.5L) deposition activity composition, first use 8% alcohol flushing, 12 column volumes (18L) to remove large polar component, then use 70% ethanol elution, 10 column volumes (15L), collect 70% eluent, concentrating under reduced pressure, obtains Radix Melastomatis Sanguinei extract and is about 120g.
Embodiment 2: extract preparation (Radix Melastomatis Sanguinei 80 parts, Radix Et Rhizoma Rhei 20 parts)
Preparation method: by the Radix Et Rhizoma Rhei dry rhizome co-grinding of the Radix Melastomatis Sanguinei of 8kg drying and 2kg, with 60% alcoholic solution circumfluence distillation (25L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution (2L) without alcohol taste; Gained ethanol extraction concentrated solution is diluted with water to 3L, petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) is used to extract successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract 212g respectively; (c) n-butyl alcohol extract 1.5L water dissolution, medical absorbent cotton filters, with AB-8 macroporous resin (2kg, column volume 1.5L) deposition activity composition, first use 8% alcohol flushing, 12 column volumes (18L) to remove large polar component, then use 70% ethanol elution, 10 column volumes (15L), collect 70% eluent, concentrating under reduced pressure, obtains Radix Melastomatis Sanguinei extract and is about 120g.
Embodiment 3: extract preparation (Radix Melastomatis Sanguinei 90 parts, Radix Et Rhizoma Rhei 10 parts)
Preparation method: by the Radix Et Rhizoma Rhei dry rhizome co-grinding of the Radix Melastomatis Sanguinei of 9kg drying and 1kg, with 60% alcoholic solution circumfluence distillation (25L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution (2L) without alcohol taste; Gained ethanol extraction concentrated solution is diluted with water to 3L, petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) is used to extract successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract 212g respectively; (c) n-butyl alcohol extract 1.5L water dissolution, medical absorbent cotton filters, with AB-8 macroporous resin (2kg, column volume 1.5L) deposition activity composition, first use 8% alcohol flushing, 12 column volumes (18L) to remove large polar component, then use 70% ethanol elution, 10 column volumes (15L), collect 70% eluent, concentrating under reduced pressure, obtains Radix Melastomatis Sanguinei extract and is about 120g.
Embodiment 4: extract preparation (Radix Melastomatis Sanguinei 75 parts, Radix Et Rhizoma Rhei 25 parts)
Preparation method: by the Radix Et Rhizoma Rhei dry rhizome co-grinding of the Radix Melastomatis Sanguinei of 7.5kg drying and 2.5kg, with 60% alcoholic solution circumfluence distillation (25L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution (2L) without alcohol taste; Gained ethanol extraction concentrated solution is diluted with water to 3L, petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) is used to extract successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract 212g respectively; (c) n-butyl alcohol extract 1.5L water dissolution, medical absorbent cotton filters, with AB-8 macroporous resin (2kg, column volume 1.5L) deposition activity composition, first use 8% alcohol flushing, 12 column volumes (18L) to remove large polar component, then use 70% ethanol elution, 10 column volumes (15L), collect 70% eluent, concentrating under reduced pressure, obtains Radix Melastomatis Sanguinei extract and is about 120g.
Embodiment 5: extract preparation (Radix Melastomatis Sanguinei 95 parts, Radix Et Rhizoma Rhei 5 parts)
Preparation method: by the Radix Et Rhizoma Rhei dry rhizome co-grinding of the Radix Melastomatis Sanguinei of 9.5kg drying and 0.5kg, with 60% alcoholic solution circumfluence distillation (25L × 3 time), merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution (2L) without alcohol taste; Gained ethanol extraction concentrated solution is diluted with water to 3L, petroleum ether (3L × 3 time), ethyl acetate (3L × 3 time) and water saturated n-butyl alcohol (3L × 3 time) is used to extract successively, concentrating under reduced pressure, obtains petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract 212g respectively; (c) n-butyl alcohol extract 1.5L water dissolution, medical absorbent cotton filters, with AB-8 macroporous resin (2kg, column volume 1.5L) deposition activity composition, first use 8% alcohol flushing, 12 column volumes (18L) to remove large polar component, then use 70% ethanol elution, 10 column volumes (15L), collect 70% eluent, concentrating under reduced pressure, obtains Radix Melastomatis Sanguinei extract and is about 120g.
Embodiment 6: liquid-phase chromatographic analysis
Need testing solution is prepared: in the brown volumetric flask of extract 5mg to 50mL that Example 1 method is obtained, add 30mL10% acetonitrile solution ultrasonic dissolution, after being cooled to room temperature, continue to add 10% acetonitrile solution standardize solution.
Analytical method:
High performance liquid chromatograph: Agilent1260, binary pump;
Chromatographic column: AgilentZorbaxSB-C18 post (4.6mm × 250mm, 5 μm);
Mobile phase: A is acetonitrile, and B is 0.4% phosphoric acid solution;
Gradient elution program: 0.01 ~ 5min, A10% → 15%; 5 ~ 15min, A15% → 48%; 15 ~ 25min, A48% → 78%; 25 ~ 40min, A78% → 10%;
Flow rate of mobile phase: 1.0mLmin
-1; Determined wavelength: 260nm; Column temperature: 30 DEG C; Sample size: 10 μ L.
Analyze with the Radix Melastomatis Sanguinei extract of 10 batches of preparation, carry out chromatographic peak coupling, 1 ~ No. 11 peak all occurs in 10 batch sample chromatograms as a result, and all reaches baseline separation with surrounding chromatographic peak.Therefore demarcate 11 peaks for total chromatographic peak, set up the HPLC quality control collection of illustrative plates of this extract accordingly, the results are shown in Figure 1.
Embodiment 7: extract pharmacological testing
One, materials and methods
1.1 medicines and reagent: Radix Melastomatis Sanguinei extract 1. ~ 5. prepare according to embodiment 1 ~ 5 respectively, be made into experimental concentration with distilled water, be placed in 4 DEG C for subsequent use; SOD test kit builds up institute of biological products by Nanjing and provides; D-galactose is provided by Shanghai reagent two factory.
1.2 laboratory animals: Kunming mouse, body weight (20 ± 2) g, provided by Anhui Province's medical test.
1.3 experimental techniques: mice 70, male and female half and half, body weight (20 ± 2) g, is divided into 7 groups at random by mice, often organize 10, i.e. Normal group, D-gal Aging model group, Radix Melastomatis Sanguinei extract 1. ~ 5. group.Except Normal group, other six treated animals nape subcutaneous injections equal every day 5%D-galactose 0.5mL, normal group mice is nape subcutaneous injection normal saline 0.5mL then.In the 10d of injection of d-galactose, Radix Melastomatis Sanguinei extract 1. ~ 5. treated animal respectively gavage give Radix Melastomatis Sanguinei extract 1. ~ 5. 200mgkg
-1, Normal group mice and aging model treated animal then every day gavage equal-volume normal saline.Continuous use 30d.In last day gavage and injection of d-galactose 2h after, eye socket gets blood, anticoagulant heparin (4 DEG C of preservations) is for the mensuration of lipid peroxide in serum (LPO) content, then sacrificed by decapitation animal, takes out cerebral tissue and makes the assay of homogenate for catalase (CAT), superoxide dismutase (SOD), peroxidase (POD).
Two, result
2.1 Radix Melastomatis Sanguinei extracts are on the impact-ultraviolet spectrophotometry of CAT activity in brain aging mice serum
Table 1 shows, and under the effect of D-galactose, the CAT in model group animal brain is active significantly to decline, and compared with Normal group, difference is very remarkable.And gavage Radix Melastomatis Sanguinei extract group 1. ~ mouse blood 3. in CAT is active obviously rises (P<0.05), higher than model group; But fill with feed Radix Melastomatis Sanguinei extract group 4. ~ mouse blood 5. in CAT active in significantly improving, illustrate Radix Melastomatis Sanguinei extract 1. ~ 3. may have protective effect to CAT activity.Note: compare * P<0.05 with model group, * * P<0.01.
Table 1 Radix Melastomatis Sanguinei extract is on the impact (X ± SD) of CAT activity in mice serum
| Group | Dosage/(mg/kg) | Protein content/mgmL -1 | CAT(k/gHb) |
| Normal group | - | 0.750 | 80.19±23.77 |
| Model group | - | 0.717 | 73.14±21.35 |
| Extract 1. | 200 | 0.701 | 90.83±8.16* |
| Extract 2. | 200 | 0.697 | 88.79±8.27* |
| Extract 3. | 200 | 0.700 | 89.75±8.13* |
| Extract 4. | 200 | 0.688 | 75.20±9.36 |
| Extract 5. | 200 | 0.691 | 76.43±8.91 |
2.2 Radix Melastomatis Sanguinei extracts are on the impact-tissue homogenate MDA colorimetry of LPO in brain aging Mice brain tissues
Table 2 shows, continuous subcutaneous injection 5% galactose aqueous solution, LPO content in Mice brain tissues is apparently higher than Normal group, illustrate that D-galactose can make cell membrane generation lipid peroxidation, and Radix Melastomatis Sanguinei extract 1. ~ the LPO content in Mice brain tissues 3. can be made to decline, with model group comparing difference obvious (P<0.05), point out it to have the effect of anti-lipid peroxidation, Radix Melastomatis Sanguinei extract 4. ~ 5. then the LPO content in Mice brain tissues is had no significant effect.Note: compare * P<0.05 with model group.
Table 2 Radix Melastomatis Sanguinei extract is on the impact (X ± SD) of LPO in Mice brain tissues
| Group | Dosage/(mg/kg) | Number of animals (only) | MDA(nmoL/mgprot) |
| Normal group | - | 10 | 1.073±0.337 |
| Model group | - | 10 | 1.131±0.175 |
| Extract 1. | 200 | 10 | 1.032±0.124* |
| Extract 2. | 200 | 10 | 1.037±0.128* |
| Extract 3. | 200 | 10 | 1.035±0.120* |
| Extract 4. | 200 | 10 | 1.127±0.133 |
| Extract 5. | 200 | 10 | 1.125±0.125 |
2.3 Radix Melastomatis Sanguinei extracts are on the impact-photo-reduction NBT method of SOD activity in brain aging Mice brain tissues
Table 3 shows, fill with feed Radix Melastomatis Sanguinei extract 1. ~ Mice brain tissues 3. in significantly improve (P<0.01) SOD active comparison with model group; And fill with feed Radix Melastomatis Sanguinei extract 4. ~ Mice brain tissues 5. in SOD active in significant change.Prompting Radix Melastomatis Sanguinei extract 1. ~ 3. have obvious potentiation to the SOD activity in mouse aging cerebral tissue.
| Group | Dosage/(mg/kg) | Number of animals (only) | SOD vigor (μ/mgprot) |
| Normal group | - | 10 | 5.393±1.444 |
| Model group | - | 10 | 3.504±1.473 |
| Extract 1. | 200 | 10 | 6.252±1.366** |
| Extract 2. | 200 | 10 | 6.247±1.351** |
| Extract 3. | 200 | 10 | 6.244±1.372** |
| Extract 4. | 200 | 10 | 3.741±1.397 |
| Extract 5. | 200 | 10 | 3.726±1.401 |
Above-mentioned experimental result shows, Radix Melastomatis Sanguinei extract has the old effect of certain anti senile.In the present invention, the content of Radix Et Rhizoma Rhei acts on most important to the anti senile of Radix Melastomatis Sanguinei extract always.
Embodiment 8: the preparation of tablet
Obtain extract by embodiment 1 method, add excipient, pelletizing press sheet in itself and excipient weight than the ratio for 1:10.
Embodiment 9: the preparation of oral liquid
By embodiment 1 method first obtained extract, oral liquid method for making makes oral liquid routinely.
Embodiment 10: the preparation of capsule or granule
By embodiment 1 method first obtained extract, add excipient in itself and excipient weight than the ratio for 1:9, make capsule or granule.
Embodiment 11: the preparation of injection
Obtain extract by embodiment 1 method, inject with water, fine straining, injection is made in embedding sterilizing.
Embodiment 12: the preparation of aseptic powder injection
By embodiment 1 method first obtained extract, be dissolved in sterile water for injection, stirring makes molten, filters with aseptic suction funnel, more aseptic fine straining, and be sub-packed in ampoule, after frozen drying, aseptic sealing by fusing obtains injectable powder.
Claims (6)
1. a Radix Melastomatis Sanguinei extract, is characterized in that described extract is prepared by following methods:
A () by weight, every 100 parts of medical materials comprise dry Radix Melastomatis Sanguinei 80 ~ 90 parts and Radix Et Rhizoma Rhei 10 ~ 20 parts, co-grinding, and with 60% alcoholic solution circumfluence distillation, merge extractive liquid, is concentrated into and obtains ethanol extraction concentrated solution without alcohol taste; B (), by step (a) gained ethanol extraction concentrated solution dilute with water, is used petroleum ether, ethyl acetate and water saturated n-butanol extraction successively, concentrating under reduced pressure, is obtained petroleum ether extract, acetic acid ethyl ester extract and n-butyl alcohol extract respectively; C () n-butyl alcohol extract water dissolution, filters, uses macroporous resin enrichment active component, first remove large polar component with 8% alcohol flushing, 12 column volumes, then use 70% ethanol elution, 10 column volumes, collect 70% eluent, concentrating under reduced pressure and get final product.
2. extract according to claim 1, is characterized in that: macroporous resin described in step (c) is AB-8 type macroporous resin.
3. extract according to claim 1, is characterized in that: the liquid phase analysis method of described extract is:
Chromatographic column: AgilentZorbaxSB-C18 post (4.6mm × 250mm, 5 μm);
Mobile phase: A is acetonitrile, and B is 0.4% phosphoric acid solution;
Gradient elution program: 0.01 ~ 5min, A10% → 15%; 5 ~ 15min, A15% → 48%; 15 ~ 25min, A48% → 78%; 25 ~ 40min, A78% → 10%;
Flow rate of mobile phase: 1.0mLmin
-1;
Determined wavelength: 260nm;
Column temperature: 30 DEG C;
Sample size: 10 μ L.
4. pharmaceutical preparation, is characterized in that: the extract according to claim 1 containing treatment effective dose and pharmaceutically acceptable carrier.
5. the application of extract according to claim 1 in the medicine that preparation treatment anti senile is old.
6. the application of pharmaceutical preparation according to claim 4 in the medicine that preparation treatment anti senile is old.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105768087A (en) * | 2016-03-30 | 2016-07-20 | 宋晓玲 | Health-care food for slowing brain aging and preparation method thereof |
| CN106074224A (en) * | 2016-06-29 | 2016-11-09 | 田东县浙缘农业科技有限公司 | A kind of removing acnes and controlling oil facial film water and preparation method thereof |
| CN106822200A (en) * | 2017-01-18 | 2017-06-13 | 中山大学 | Hair harvests the preparation technology and its antioxidation application of fruit extract |
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| CN103800386A (en) * | 2014-02-10 | 2014-05-21 | 江苏大学 | Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105768087A (en) * | 2016-03-30 | 2016-07-20 | 宋晓玲 | Health-care food for slowing brain aging and preparation method thereof |
| CN106074224A (en) * | 2016-06-29 | 2016-11-09 | 田东县浙缘农业科技有限公司 | A kind of removing acnes and controlling oil facial film water and preparation method thereof |
| CN106822200A (en) * | 2017-01-18 | 2017-06-13 | 中山大学 | Hair harvests the preparation technology and its antioxidation application of fruit extract |
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