CN105044232A - Method used for determining puff-by-puff alkaloids in cigarette main stream smoke - Google Patents
Method used for determining puff-by-puff alkaloids in cigarette main stream smoke Download PDFInfo
- Publication number
- CN105044232A CN105044232A CN201510388049.3A CN201510388049A CN105044232A CN 105044232 A CN105044232 A CN 105044232A CN 201510388049 A CN201510388049 A CN 201510388049A CN 105044232 A CN105044232 A CN 105044232A
- Authority
- CN
- China
- Prior art keywords
- cigarette
- puff
- solenoid valve
- suction
- smoking machine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a method used for determining puff-by-puff alkaloids in cigarette main stream smoke. According to the method, an improved smoking machine is adopted; cigarettes to be tested are smoked successively; magnetic valves on a gas circuit are turned on respectively; particular matters in the smoke of the cigarettes to be tested are collected by total particular matter filter element collectors; filter elements are subjected to ultrasonic extraction and centrifugation, an obtained supernatant liquid is subjected to GC/MS analysis; and internal standard method quantitative analysis is carried out using five alkaloid standard samples. The improved smoking machine is provided with ten magnetic valves and ten total particular matter filter element collectors; smoking processes of the improved smoking machine is controlled by a control system; collection of the particular matters of each puff of twenty cigarettes is realized; the method high in repeatability and recovery rate when the method is used for determining alkaloids of particulates of smoke gas each puff; determination is convenient; efficiency is high; and practicality is high.
Description
Technical field
The invention belongs to cigarette quality detection field, be specifically related to a kind of mensuration in cigarette mainstream flue gas by the alkaloidal method of mouth.
Background technology
Alkaloid is the important component affecting quality of tobacco, and except nicotine, alkaloid main in tobacco also comprises nornicotine, myosmine, anabasine and anabasine.Nicotine directly can stimulate human central nervous, produces physiological reaction; Nornicotine has negative effect to cigarette odor-absorbing; Myosmine has a kind of mouse smell, has a strong impact on the suction taste of cigarette product; The toxicity of anabasine is slightly lower than nicotine.Alkaloid is one of direct precursor thing of tobacco-specific nitrosamine, easy metabolism is burnt and sucked in process for falling alkaloid in tobacco modulation and cigarette, and then be formed with the tobacco-specific nitrosamine of carcinogenicity with nitrite, especially nicotine transforms to nornicotine is one of principal element causing tobacco leaf NNN and total TSNA content to increase.Therefore, alkaloid in research cigarette mainstream flue gas is the important measures that cigarette quality controls.
Alkaloid in current cigarette mainstream flue gas generally by measure, in Simultaneously test cigarette mainstream smoke condensate five kinds of alkaloids (nicotine, nornicotine, myosmine, anabasine and anabasine) by sucking quantifier elimination, there is not been reported.
Summary of the invention
The object of this invention is to provide and a kind of measure by the alkaloidal method of mouth in cigarette mainstream flue gas, can five kinds of alkaloids be by the amount of sucking in Simultaneously test cigarette mainstream smoke condensate, simple to operate, detection efficiency is high, reproducible.
To achieve these goals, the present invention adopts following technical scheme: by the alkaloidal method of mouth in a kind of mensuration cigarette mainstream flue gas, comprise the steps:
(1) sample prepares
According to GB/T5606.1-2004 cigarette sampling standard, the cigarette of the same batch of same trade mark is positioned over temperature 22 ± 1 DEG C, in the climatic chamber of relative humidity 60 ± 3%, balances more than 48h, then carry out the screening of resistance to suction and weight;
(2) sample collection
By GB/T16450-2004 national standard and ISO3308:2012 tobacco business standard regulation, use the semi-automatic smoking machine of improved RM20 type temperature 22 ± 2 DEG C, aspirate in the environment of relative humidity 60 ± 5%, each takes turns 20 cigarette of suction same sample, each 35 ± the 0.3mL of pumping volume, puff duration 2s, suction frequencies 60s; During suction, gas circuit one solenoid valve opened first automatically by smoking machine, starts electronic air extracting pump and aspirates, and 20 cigarette first granule phase substances are collected in corresponding TPM filter disc drip catcher; After the first of 20 cigarette all aspirates and terminates, smoking machine stops electronic air extracting pump automatically, close gas circuit one solenoid valve, open gas circuit two solenoid valve, second takes turns suction starts automatically, second take turns suction after, 20 cigarette, second mouthful of granule phase substance is collected in corresponding TPM filter disc drip catcher; When 20 cigarette second mouthful, all suction is complete, stops electronic air extracting pump, closes gas circuit two solenoid valve, open gas circuit three solenoid valve, restart electronic air extracting pump, the like, until cigarette all sucks, trap the granule phase substance composition in each single port cigarette smoke with the cambridge filter of diameter 44mm;
(3) sample analysis
The cambridge filter of each single port granule phase substance of trapping is placed in 50mL conical flask respectively, add 100 μ l inner mark solutions, 2mL5wt% sodium hydroxide solution and 15mL ethyl acetate, vibration shakes up, conical flask is put into supersonic wave cleaning machine supersonic extraction, solution all being transferred to centrifuge tube carries out centrifugal, get supernatant liquor after organic membrane filtration in 0.45 μm of aperture, inject 2mL gas chromatography auto injection bottle, carry out determination and analysis with GC/MS;
(4) GC/MS instrumental analysis
1. GC analysis condition: chromatographic column adopts Agilent19091B-105Ultra2, and Stationary liquid is 5% phenyl-95% dimethyl polysiloxane, 50.0m*2.0mm*0.33 μm; Injector temperature 250 DEG C; With high-purity helium for carrier gas, flow is 1.8mL/min; Adopt split sampling mode, split ratio is 10:1, and sample size is 1 μ L; Heating schedule: initial temperature 100 DEG C, keeps 1min, is elevated to 180 DEG C with 6 DEG C/min heating rate, keeps 2min, then is raised to 240 DEG C with 12 DEG C/min heating rate, keeps 10min, rear running temperature 260 DEG C, keeps 4min;
2. MS analysis condition: ionization mode adopts electron ionization sources; Ionization voltage 70ev; Ion source temperature 230 DEG C; Level Four bar temperature 150 DEG C; MSD transmission line temperature 280 DEG C; Adopt full scan and Selective ion mode scan mode; NIST picture library;
(5) adopt n-compound retention time qualitative, and adopt the qualitative checking of NIST mass spectrum picture library further; Adopt five kinds of alkaloid standard models to carry out inner mark method ration to nicotine, nornicotine, myosmine, anabasine, anabasine respectively, obtain each alkaloid of total particulate matter in mainstream smoke.
Further, described smoking machine is provided with ten TPM filter disc drip catchers, and described drip catcher backup block upper end is uniformly distributed ten two-way electromagnetic valves, and one end of solenoid valve is communicated with smoke absorbing end, the other end of solenoid valve is communicated with one end of drip catcher, and the drip catcher other end is communicated with electronic air extracting pump.
Further, described smoking machine adopts PLC control system.
Further, inner mark solution described in step (3) is take ethyl acetate as solvent, and concentration is 2 of 2000 μ g/mL, 4 '-dipyridine.
Further, the supersonic extraction time described in step (3) is 20min, and ultrasonic frequency is 40kHz, and ultrasonic power is 500W.
The present invention is by ten solenoid valves that smoking machine is arranged and ten TPM filter disc drip catchers, the aspiration procedure of smoking machine is controlled through control system, achieve the trapping of the TPM once aspirating the every single port namely completing 20 cigarette, five kinds of alkaloid (nicotine, nornicotine, myosmine, anabasine and anabasine) content in energy Simultaneously test cigarette smoke condensates, adopt the present invention to measure each single port alkaloid in Smoke Particulate and there is repeatability and the higher recovery preferably, convenient test efficiency is high, practical.
Accompanying drawing explanation
Fig. 1 is schematic diagram of gas circuit of the present invention.
In figure, 1, smoke absorbing end; 2, solenoid valve; 3, drip catcher; 4, electronic air extracting pump.
Embodiment
One, the cigarette sample, main agents and the instrument that use of the present embodiment
(1) cigarette sample is in table 1
Table 1 cigarette sample and specification (mg/ props up)
| Cigarette sample | Box mark tar | Box mark nicotine | Box mark carbon monoxide |
| A | 13 | 1.3 | 13 |
| B | 8 | 0.8 | 8 |
| C | 5 | 0.6 | 8 |
| D | 8 | 0.8 | 10 |
| E | 13 | 1.2 | 13 |
(2) standard model.Nicotine, nornicotine, myosmine, anabasine, anabasine, 2,4 '-dipyridine (interior mark), above standard specimen is chromatographically pure level, is purchased from AcrosOrganics company of the U.S., and all standard specimens are all kept at lower than 4 DEG C of refrigerators for subsequent use;
(3) reagent.Ethyl acetate (chromatographically pure, SIGMA company), 5wt% sodium hydroxide solution, inner mark solution (concentration is 2 of 2000 μ g/mL, 4 '-dipyridine, take ethyl acetate as solvent);
(4) instrument.RM20/CS rotating disc type smoking machine (German Borgwaldt company, through transformation); HP6890-HP5973GC/MS gas chromatograph-mass spectrometer (Agilent company of the U.S.); Analytical balance (sensibility reciprocal 0.1mg, plum Teller-Tuo benefit group of Switzerland); TD5G hydro-extractor (Kai Da hydro-extractor company); KQ-500 type supersonic wave cleaning machine (Kunshan Ultrasonic Instruments Co., Ltd., ultrasonic frequency is 40kHz, and ultrasonic power is 500W); HY-2 type velocity-modulated oscillator (Community of Jin Tan County the earth self-reacting device factory); Direct-Q5 ultrapure water generator (MILLIPORE company of the U.S.).
As shown in Figure 1, described smoking machine is provided with ten TPM filter disc drip catchers 3, described drip catcher 3 backup block upper end is uniformly distributed ten two-way electromagnetic valves 2, one end of solenoid valve 2 is communicated with smoke absorbing end 1, the other end of solenoid valve 2 is communicated with one end of drip catcher 3, for switching from the mainstream smoke total particulate matter of smoking machine smoke absorbing end 1 aspiration procedure and each drip catcher 3 one_to_one corresponding.Drip catcher 3 other end is communicated with electronic air extracting pump 4.
Described smoking machine adopts PLC control system to control the aspiration procedure of smoking machine.
Two, the concrete method of testing of the present embodiment, comprises the following steps:
(1) sample prepares
According to GB/T5606.1-2004 cigarette sampling standard, the cigarette of the same batch of same trade mark is positioned over temperature 22 ± 1 DEG C, in the climatic chamber of relative humidity 60 ± 3%, balances more than 48h, then carry out the screening of resistance to suction and weight;
(2) sample collection
By GB/T16450-2004 national standard and ISO3308:2012 tobacco business standard regulation, use the semi-automatic smoking machine of improved RM20 type temperature 22 ± 2 DEG C, aspirate in the environment of relative humidity 60 ± 5%, each takes turns 20 cigarette of suction same sample, each 35 ± the 0.3mL of pumping volume, puff duration 2s, suction frequencies 60s; During suction, gas circuit one solenoid valve 2 opened first automatically by smoking machine, starts electronic air extracting pump 4 and aspirates, and 20 cigarette first granule phase substances to collect in corresponding TPM filter disc drip catcher 3; After the first of 20 cigarette all aspirates and terminates, smoking machine stops electronic air extracting pump 4 automatically, close gas circuit one solenoid valve 2, open gas circuit two solenoid valve 2, second takes turns suction starts automatically, second take turns suction after, 20 cigarette, second mouthful of granule phase substance is collected in corresponding TPM filter disc drip catcher 3; When 20 cigarette second mouthful, all suction is complete, stops electronic air extracting pump 4, closes gas circuit two solenoid valve 2, open gas circuit three solenoid valve 2, restart electronic air extracting pump 4, the like, until cigarette all sucks, trap the granule phase substance composition in each single port cigarette smoke with the cambridge filter of diameter 44mm;
(3) sample analysis
The cambridge filter of each single port granule phase substance of trapping is placed in 50mL conical flask respectively, add 100 μ L inner mark solutions, 2mL5wt% sodium hydroxide solution and 15mL ethyl acetate, vibration shakes up, conical flask is put into supersonic wave cleaning machine supersonic extraction 20min, solution all being transferred to centrifuge tube carries out centrifugal, get supernatant liquor after organic membrane filtration in 0.45 μm of aperture, inject 2mL gas chromatography auto injection bottle, carry out determination and analysis with GC/MS;
(4) GC/MS instrumental analysis
1. GC analysis condition: chromatographic column adopts Agilent19091B-105Ultra2, and Stationary liquid is 5% phenyl-95% dimethyl polysiloxane, 50.0m*2.0mm*0.33 μm; Injector temperature 250 DEG C; With high-purity helium for carrier gas, flow is 1.8mL/min; Adopt split sampling mode, split ratio is 10:1, and sample size is 1 μ L; Heating schedule: initial temperature 100 DEG C, keeps 1min, is elevated to 180 DEG C with 6 DEG C/min heating rate, keeps 2min, then is raised to 240 DEG C with 12 DEG C/min heating rate, keeps 10min, rear running temperature 260 DEG C, keeps 4min;
2. MS analysis condition: ionization mode adopts electron ionization sources; Ionization voltage 70ev; Ion source temperature 230 DEG C; Level Four bar temperature 150 DEG C; MSD transmission line temperature 280 DEG C; Adopt full scan and Selective ion mode scan mode; NIST picture library;
(5) qualitative and quantitative
1. qualitative: to adopt n-compound retention time directly qualitative, and further Mass Spectrometric Identification, NIST signals assigned is adopted to the qualification of mass spectrogram;
2. quantitative: adopting alkaloid standard model to carry out inner mark method ration to nicotine, nornicotine, myosmine, anabasine, these five kinds of alkaloids of anabasine respectively, is interior mark with 2,4 '-dipyridine.
The present embodiment determines the main flume of 5 kinds of trade mark cigarette by 5 kinds of alkaloidal amounts in mouth granule phase substance, and analysis result is in table 2.
Table 2 cigarette sample main flume is by alkaloid measurement result in mouth granule phase substance
Three, the mensuration of detectability and quantitative limit
Get the alkaloid standard solution 2mL of least concentration, repeat sample introduction 10 times, obtain standard deviation (SD), by detectability and the quantitative limit of 3SD and 10SD computing method, in table 3.
The detectability of table 3 method and quantitative limit
Note: detectability (LOD)=3 times standard deviation; Quantitative limit (LOQ)=10 times standard deviation
Four, repeatability and sample recovery of standard addition
Aspirated specimens B cigarette under the same conditions, collect the 4th mouthful of mainstream smoke total particulate matter, often organize suction 20, be collected on 1 cambridge filter, GC-MS mensuration is carried out respectively according to the alkaloid of the method for the invention to the 4th mouthful of Smoke Particulate, parallelly make 5 samples, calculate the relative standard deviation of 5 measurement results, in table 4.
The repeatability (μ g/ props up) (n=5) of table 4 method
As can be seen from Table 4, in measured 5 in alkaloid, the relative standard deviation of 5 measurement results is 1.33% ~ 7.58%, illustrates that the present invention is better to the repeatability of the pre-treating method of cigarette sample, is suitable for cigarette smoke by quantitative test alkaloidal in mouth TPM.
Adopt standard addition method, in extraction flask, add standard specimen, carry out recovery experiment by assay method, replicate determination 5 times, the results are shown in Table 5.
The recovery (n=5) of table 5 method
| Alkaloid title | Average content (μ g) | Addition (μ g) | Measured quantity (μ g) | The recovery (%) |
| Nicotine | 3898.12 | 4544 | 8533.17 | 101.08％ |
| Nornicotine | 9.30 | 11.5 | 20.54 | 98.75％ |
| Myosmine | 7.81 | 20.3 | 26.99 | 96.01％ |
| Anabasine | 4.42 | 117.8 | 125.68 | 102.83％ |
| Anabasine | 0.99 | 10.2 | 11.37 | 101.61％ |
As can be seen from Table 5: each alkaloidal recovery of standard addition, all between 96% ~ 102%, illustrates that this results contrast is accurate, be applicable to alkaloidally analyzing by mouth quantitative measurement.
Claims (5)
1. to measure in cigarette mainstream flue gas by the alkaloidal method of mouth, it is characterized in that, comprise the following steps:
1) according to GB/T5606.1-2004 cigarette sampling standard, the cigarette of the same batch of same trade mark is positioned over temperature 22 ± 1 DEG C, in the climatic chamber of relative humidity 60 ± 3%, balances more than 48h, then carry out the screening of resistance to suction and weight;
2) by GB/T16450-2004 national standard and ISO3308:2012 tobacco business standard regulation, use the semi-automatic smoking machine of improved RM20 type temperature 22 ± 2 DEG C, aspirate in the environment of relative humidity 60 ± 5%, each takes turns 20 cigarette of suction same sample, each 35 ± the 0.3mL of pumping volume, puff duration 2s, suction frequencies 60s; During suction, gas circuit one solenoid valve opened first automatically by smoking machine, starts electronic air extracting pump and aspirates, and 20 cigarette first granule phase substances are collected in corresponding TPM filter disc drip catcher; After the first of 20 cigarette all aspirates and terminates, smoking machine stops electronic air extracting pump automatically, close gas circuit one solenoid valve, open gas circuit two solenoid valve, second takes turns suction starts automatically, second take turns suction after, 20 cigarette, second mouthful of granule phase substance is collected in corresponding TPM filter disc drip catcher; When 20 cigarette second mouthful, all suction is complete, stops electronic air extracting pump, closes gas circuit two solenoid valve, open gas circuit three solenoid valve, restart electronic air extracting pump, the like, until cigarette all sucks, trap the granule phase substance composition in each single port cigarette smoke with the cambridge filter of diameter 44mm;
3) cambridge filter of each single port granule phase substance of trapping is placed in 50mL conical flask respectively, add 100 μ L inner mark solutions, 2mL5wt% sodium hydroxide solution and 15mL ethyl acetate, vibration shakes up, conical flask is put into supersonic wave cleaning machine supersonic extraction, solution all being transferred to centrifuge tube carries out centrifugal, get supernatant liquor after organic membrane filtration in 0.45 μm of aperture, inject 2mL gas chromatography auto injection bottle, carry out determination and analysis with GC/MS;
4) GC/MS instrumental analysis
1. GC analysis condition: chromatographic column adopts Agilent19091B-105Ultra2, and Stationary liquid is 5% phenyl-95% dimethyl polysiloxane, 50.0m*2.0mm*0.33 μm; Injector temperature 250 DEG C; With high-purity helium for carrier gas, flow is 1.8mL/min; Adopt split sampling mode, split ratio is 10:1, and sample size is 1 μ L; Heating schedule: initial temperature 100 DEG C, keeps 1min, is elevated to 180 DEG C with 6 DEG C/min heating rate, keeps 2min, then is raised to 240 DEG C with 12 DEG C/min heating rate, keeps 10min, rear running temperature 260 DEG C, keeps 4min;
2. MS analysis condition: ionization mode adopts electron ionization sources; Ionization voltage 70ev; Ion source temperature 230 DEG C; Level Four bar temperature 150 DEG C; MSD transmission line temperature 280 DEG C; Adopt full scan and Selective ion mode scan mode; NIST picture library;
5) adopt n-compound retention time qualitative, and adopt the qualitative checking of NIST mass spectrum picture library further; Adopt five kinds of alkaloid standard models to carry out inner mark method ration to nicotine, nornicotine, myosmine, anabasine, anabasine respectively, obtain each alkaloid of total particulate matter in mainstream smoke.
2. in mensuration cigarette mainstream flue gas according to claim 1 by the alkaloidal method of mouth, it is characterized in that, described smoking machine is provided with ten TPM filter disc drip catchers, described drip catcher backup block upper end is uniformly distributed ten two-way electromagnetic valves, one end of solenoid valve is communicated with smoke absorbing end, the other end of solenoid valve is communicated with one end of drip catcher, and the drip catcher other end is communicated with electronic air extracting pump.
3. in mensuration cigarette mainstream flue gas according to claim 1 and 2 by the alkaloidal method of mouth, it is characterized in that, described smoking machine adopts PLC control system.
4. in mensuration cigarette mainstream flue gas according to claim 1 and 2 by the alkaloidal method of mouth, it is characterized in that, inner mark solution described in step 3 is take ethyl acetate as solvent, and concentration is 2 of 2000 μ g/mL, 4 '-dipyridine.
5. in mensuration cigarette mainstream flue gas according to claim 1 and 2 by the alkaloidal method of mouth, it is characterized in that, the time of supersonic extraction described in step 3 is 20min, and ultrasonic frequency is 40kHz, and ultrasonic power is 500W.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510388049.3A CN105044232A (en) | 2015-07-03 | 2015-07-03 | Method used for determining puff-by-puff alkaloids in cigarette main stream smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510388049.3A CN105044232A (en) | 2015-07-03 | 2015-07-03 | Method used for determining puff-by-puff alkaloids in cigarette main stream smoke |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105044232A true CN105044232A (en) | 2015-11-11 |
Family
ID=54450941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510388049.3A Pending CN105044232A (en) | 2015-07-03 | 2015-07-03 | Method used for determining puff-by-puff alkaloids in cigarette main stream smoke |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105044232A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290458A (en) * | 2017-08-23 | 2017-10-24 | 国家烟草质量监督检验中心 | It is a kind of while determining the method for 12 kinds of alkaloids in tobacco and tobacco product |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201155988Y (en) * | 2008-01-25 | 2008-11-26 | 王树喜 | Fume collector automatic gauging device |
| CN201749074U (en) * | 2010-03-16 | 2011-02-16 | 中国烟草总公司郑州烟草研究院 | Device capable of realizing real-time measurement of cigarette smoke aerosol |
| CN102004132A (en) * | 2010-10-20 | 2011-04-06 | 中国烟草总公司郑州烟草研究院 | Method for measuring alkaloid in tobacco and tobacco product |
| CN102109393A (en) * | 2010-11-30 | 2011-06-29 | 中国烟草总公司郑州烟草研究院 | Cigarette ignition temperature distribution detector and automatic control method thereof |
| CN104237420A (en) * | 2014-10-15 | 2014-12-24 | 云南中烟工业有限责任公司 | Method for building cigarette bitter taste index evaluation model based on taste activity value |
| CN104698102A (en) * | 2015-03-17 | 2015-06-10 | 川渝中烟工业有限责任公司 | Method of measuring nicotine content in cigarette main smoke condensates |
-
2015
- 2015-07-03 CN CN201510388049.3A patent/CN105044232A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201155988Y (en) * | 2008-01-25 | 2008-11-26 | 王树喜 | Fume collector automatic gauging device |
| CN201749074U (en) * | 2010-03-16 | 2011-02-16 | 中国烟草总公司郑州烟草研究院 | Device capable of realizing real-time measurement of cigarette smoke aerosol |
| CN102004132A (en) * | 2010-10-20 | 2011-04-06 | 中国烟草总公司郑州烟草研究院 | Method for measuring alkaloid in tobacco and tobacco product |
| CN102109393A (en) * | 2010-11-30 | 2011-06-29 | 中国烟草总公司郑州烟草研究院 | Cigarette ignition temperature distribution detector and automatic control method thereof |
| CN104237420A (en) * | 2014-10-15 | 2014-12-24 | 云南中烟工业有限责任公司 | Method for building cigarette bitter taste index evaluation model based on taste activity value |
| CN104698102A (en) * | 2015-03-17 | 2015-06-10 | 川渝中烟工业有限责任公司 | Method of measuring nicotine content in cigarette main smoke condensates |
Non-Patent Citations (8)
| Title |
|---|
| 丁超 等: "主流烟气中烟碱焦油和一氧化碳的逐口递送规律", 《农产品加工》 * |
| 丁超 等: "卷烟主流烟气粒相物中逐口生物碱含量测定及其递送规律", 《烟草化学》 * |
| 中华人民共和国国家质量监督检验检疫总局，中国国家标准化管理委员会: "《GB-T 5606.1-2004 卷烟 第1部分抽样》", 14 December 2014 * |
| 中华人民共和国国家质量监督检验检疫总局: "《GBT 16450-2004 常规分析用吸烟机定义和标准条件》", 14 December 2004 * |
| 国际标准组织（INTERNATIONAL ORGANIZATION FOR STANDARDIZATION）: "《ISO 3308-2012》", 15 October 2012 * |
| 李超: "卷烟主流烟气中6种化学成分的逐口释放量分析", 《烟草科技》 * |
| 沈晓晨: "逐口主流烟气粒相物中氨与烟气常规成分的相关性", 《烟草科技》 * |
| 陈建华: "GC．MS内标法分析测定晒黄烟烟叶中5种生物碱的含量", 《光谱实验室》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107290458A (en) * | 2017-08-23 | 2017-10-24 | 国家烟草质量监督检验中心 | It is a kind of while determining the method for 12 kinds of alkaloids in tobacco and tobacco product |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105067716A (en) | Method for determining puff-by-puff nicotine and tar in cigarette mainstream smoke | |
| CN103257196B (en) | Method for detecting alkaloids and nitrosamines in tobaccos simultaneously | |
| CN102012407B (en) | Method for detecting tobacco-specific nitrosamines | |
| CN102004132B (en) | Method for measuring alkaloid in tobacco and tobacco product | |
| CN104655778B (en) | A kind of method for measuring 18 kinds of volatilizations and semi-volatile organic matter content in tobacco juice for electronic smoke and aerosol | |
| CN105675757B (en) | It is a kind of at the same determine cigarette mainstream flue gas in the peculiar N nitrosamine of tobacco and polycyclic aromatic hydrocarbon method | |
| CN106885857B (en) | The analysis method of nicotine chiral isomer in a kind of tobacco and flue gas | |
| CN102565231B (en) | Method for determining eight kinds of alkaloid in cured tobacco through gas chromatography-nitrogen chemoluminescence detection method | |
| CN103293245B (en) | GC-MS (Gas Chromatography-Mass Spectrometer)/MS (Mass Spectrometer) method for simultaneously analyzing four tobacco-specific nitrosamines (TSNAs) in main stream smoke of cigarette | |
| CN103257194B (en) | GC-MS/MS (Gas chromatography - tandem mass spectrometry) method for simultaneously analyzing three polycyclic aromatic hydrocarbons and four tobacco-specific nitrosamines in main stream smoke of cigarettes | |
| CN104133031A (en) | Method and device for determination of cigarette smoke benzo[alpha]pyrene by on-line solid phase extraction | |
| CN105372354A (en) | Method for detecting nicotine and secondary alkaloids in nicotine liquid and main stream smoke of electronic cigarette | |
| CN104237431A (en) | Method for measuring TSNAs (tobacco-specific nitrosamines) in electronic cigarette smoke | |
| CN107561182A (en) | Volatility and the detection method of semi-volatile organic compounds in cigarette mainstream flue gas | |
| CN102353741A (en) | Method for measuring contents of four kinds of tobacco-specific nitrosamines in cigarette mainstream smoke | |
| CN105527356B (en) | The peculiar N nitrosamine of tobacco and assay method while polycyclic aromatic hydrocarbon in a kind of cigarette mainstream flue gas based on suction nozzle micro-extraction | |
| CN104142374A (en) | Method for measuring content of carbonyl compound in electronic tobacco juice by adopting direct derivation/high performance liquid chromatography | |
| CN110320292A (en) | The measuring method of volatile component in a kind of full flue gas of electronic cigarette | |
| CN106442753A (en) | Method for measuring content of TSNAs (tobacco-specific nitrosamines) in mainstream smoke of cigarettes | |
| CN106841444A (en) | A kind of method of quantitative analysis ramuscule cigarette main flow by main Alkaloid in mouth flue gas | |
| CN108845064A (en) | A kind of full smoke pH method for measuring of cigarette mainstream | |
| CN108931596A (en) | Method that is a kind of while detecting acetic acid and nicotine in cigarette mainstream flue gas | |
| CN105044232A (en) | Method used for determining puff-by-puff alkaloids in cigarette main stream smoke | |
| CN106093235B (en) | It is a kind of for measuring the extract liquor and its application method of moisture and nicotine content in cigarette mainstream flue gas | |
| CN103698433A (en) | Method for detecting chloroethylene in sidestream smoke of cigarettes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151111 |
|
| WD01 | Invention patent application deemed withdrawn after publication |