CN105031612B - A kind of endoplasmic reticulum albumen of homocysteine induction is preparing the application in treating or preventing EV71 infection medicines - Google Patents
A kind of endoplasmic reticulum albumen of homocysteine induction is preparing the application in treating or preventing EV71 infection medicines Download PDFInfo
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- CN105031612B CN105031612B CN201510398925.0A CN201510398925A CN105031612B CN 105031612 B CN105031612 B CN 105031612B CN 201510398925 A CN201510398925 A CN 201510398925A CN 105031612 B CN105031612 B CN 105031612B
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Abstract
The present invention discloses a kind of application of endoplasmic reticulum albumen of homocysteine induction in treatment or prevention EV71 infection medicines are prepared, and its step is：A utilizes pCMV Tag Flag2B vector construction pCMV HERP plasmids；Detection mark of the VP1 structural proteins and 3C non-structural proteins of B EV71 viruses as EV71 virus replications, and devise fluorogenic quantitative detection primer for VP1 GFPs；C time gradients are tested.Expression HERP albumen can substantially suppress the mRNA level in-site of EV71 virus VP 1s, and the dose-dependent effect of gradient reduction is presented with the raising of HERP plasmid concentrations.The expression that immunoblot experiment also show VP1 and 3C albumen reduces also with the raising of HERP expressing quantities.
Description
Technical field
The present invention relates to antiviral drugs field, is related to the endoplasmic reticulum albumen that homocysteine induces
(homocysteine-induced endoplasmic reticulum protein,HERP)(GenBank accession
No.NM_001010989 new application), it is more particularly to a kind of endoplasmic reticulum albumen of homocysteine induction and is preparing treatment
Or the application in prevention enterovirns type 71 (EV71) virus infective medicament.
Background technology
Enterovirns type 71 (Enterovirus 71, EV71) belongs to micro ribonucleic acid (tiny RNA) Viraceae
(Picornaradae) enterovirus genus (Enterovirus), it is the single strand RNA virus of a normal chain.EV71 is to cause baby young
One of youngster's hand-foot-and-mouth disease (hand, foot and mouth disease, HFMD) most important pathogen, serious EV71 infection
Some the nervous system diseases, such as BBE, aseptic meningitis and acute flaccid paralysis etc. can also be caused.From 1969
EV71 is found first and separation obtained first in 1974, and EV71 causes large-scale hand all over the world in infant
All there is breaking out for EV71 epidemic situations in sufficient stomatosis outbreak of epidemic, including the U.S., Europe and the Asian-Pacific area, and TaiWan, China was from 1998
There have been EV71 epidemic situations for beginning.And it is because complication caused by central nervous system and thing followed high mortality, draw
Highly vigilant of and the concern of countries in the world are played.Recent years, EV71 infection broke out frequently in China, to health and public
Safety all causes huge threat.
Hand-foot-and-mouth disease caused by EV71 viruses in Chinese outbreak of epidemic is mainly the C4 hypotypes of EV71 viruses, and exists
The mixed infection of EV71 and Coxsackie virus (CVA16) and periodicity are broken out.Although cause the viral fatal rate broken out in China
It is not high, but its height break out rate still need it is vigilant.Moreover, so far still without the safety for EV71 virus exploitations
Effective viral infection resisting medicine or vaccine are approved to list.In addition to natural prodcuts or compound, for EV71 viruses
Duplication feature and life cycle, exploitation medicine mainly have it is following strategy and molecular target.
Neutralizing antibody：The monoclonal antibody of EV71 viruses, by the viruslike particle infecting mouse of EV71 viruses, obtain disease
The monoclonal antibody of poison, it can specifically be combined with EV71 viruses, change the structure of virus, induce virus genomic release,
So as to infection of the blocking virus to host cell.
For poisoning intrusion：It is an antiviral available strategy to prevent poisoning intrusion cell.Some medicines are thin in infection
Born of the same parents before be incubated jointly with EV71 virions, by combine EV71 virus capsid structure, prevent virus with cell receptor it
Between combination, can substantially reduce virus invasion and progeny virus generation.These medicines can combine EV71 virions, lead to
Cross and prevent viral binding partner, block invasion, suppress EV71 virus uncoatings etc., by combine the specific structure on capsid protein come
Developed.
For virus protein：The Structural protein VP1 of EV71 viruses, combinations and duplication of the NS2 Protein A and 3C in virus
During play the role of important, therefore some specific small-molecule drugs are developed, and these medicines can be specific
With reference to virus protein (such as VP1), or suppress the proteinase activity (such as 2A and 3C) of non-structural protein.
For viral replication complex：The duplication of virus needs to rely on the RdRP replication complexs of 3D polymerases composition, because
This, it is suppressed that the replication activity of 3D polymerases, also just reach and suppressed the purpose that viral genome replicates, so as to blocking virus
Progression of infection.
For Virus translation：Because the geneome RNA of EV71 viruses depends on IRES without 5 ' end cap sequences, its duplication
Element, and receive ITAFs regulation and control, and it is reported that FBP2 albumen can be in emulative combination ITAFs PTB, so as to suppress
IRES activity, so as to realize the effect of the duplication and the translation that suppress EV71 viruses.
In addition, also some other mode may can suppress the duplication of EV71 viruses, such as RNA interference, regulation host
Immune system and with interferon therapy etc..
HERP albumen is also referred to as SUP (Stress-associated Ubiquitin-like Protein), Mif1
(methyl methanesulphonate (MMS)-inducible fragment1) or HERPUD1, encode 391 amino acid.
The expression of HERP albumen is regulated and controled by endoplasmic reticulum stress response, has a variety of cis elements and tune in er stress regulation and control HERP
The factor is controlled to participate in, can the Non-adhesion inhibition index caused by homocysteine activate, and because it is positioned in endoplasmic reticulum,
Therefore it is referred to as endoplasmic reticulum albumen (the homocysteine-induced endoplasmic of homocysteine induction
reticulum protein,HERP).Under the irradiation of both tunicamycin treatment, osmotic shock and ultraviolet, it can equally promote its table
Reach.
HERP is the ubiquitin being positioned in an endoplasmic reticulum sample memebrane protein, 391 amino acid of HERP encoding histones, in its N
End has the similar domain (ubiquitin-like domain, UBL domain) of a ubiquitination, includes N-terminal
1-87 amino acids, and there is ubiquitination function.HERP 240-391 amino acids then form C-terminal, wherein including transmembrane region
Domain, HERP albumen is set to be positioned on endoplasmic reticulum, its N-terminal and C-terminal are in the cytoplasm face of endoplasmic reticulum.
HERP function includes participating in protein degradation, maintains Ca2+Homeostasis, and it is related to some diseases.Almost institute
The presence of HERP albumen can be detected in somebody's body tissue.HERP albumen can the endoplasm caused by Non-adhesion inhibition index etc.
Net stress reaction raises, and participates in protecting cell reply er stress.Current research finds that HERP is the one of ubiquitin family
One of member, its function are to transport Endoplasmic Reticulum Associated Protein Degradation (ERAD) substrate, and ubiquitination substrate is transported into 26S eggs
Degraded in white enzyme body.
In endoplasmic reticulum stress response, HERP albumen can also help cell to maintain Ca2+Homeostasis.In nerve cell,
HERP can be regulated and controled by er stress, and when endoplasmic reticulum stress response causes Apoptosis, HERP can stablize in endoplasmic reticulum
Ca2+Horizontal, protection mitochondrial function and the activity for suppressing caspase 3, endoplasmic reticulum pressure is reduced, so as to improve the existence of cell
Ability, avoid that Apoptosis occurs.
In Kieren Perkins patient black substance and Lewy body, HERP expression quantity is significantly raised, prompt it in nerve degeneration and
Effect in neuroinflamation, HERP can rely on Ubiquitin-proteasome degradation pathway and maintain Ca in Kieren Perkins disease2+Balance and prevent
Apoptosis.And in two type alcoholic patients, influenceed by Alcoholism, HERP mRNA level in-site is in neuroprotection cell
In also obvious rise.Correlative study in systemic loupus erythematosus shows that HERP also has the ability that anti-DNA antisense is answered.
But there is presently no the reported in literature of relation between HERP and viral disease and correlated virus, also without HERP to present
The more report of the prevention and treatment of popular and stronger pathogenicity enterovirns type 71 (EV71).
The content of the invention
The purpose of the present invention is to be the provision of a kind of endoplasmic reticulum albumen (HERP) of homocysteine induction preparing
Treat or prevent the application in enterovirns type 71 (EV71) medicine.Homocysteine is induced from cell and molecular level
The anti-EV71 virus functions of endoplasmic reticulum albumen (HERP) are evaluated, and are had laid a good foundation for its further development and application.
The purpose of the present invention is achieved through the following technical solutions：
A kind of endoplasmic reticulum albumen of homocysteine induction is preparing the application in treating or preventing EV71 infection medicines,
Its step is：
A, built using pCMV-Tag-Flag2B carriers (mammalian expression vector, Agilent Technologies)
PCMV-HERP plasmids.
B, to the sensitive pernicious embryonal rhabdomyosarcoma cell of people of EV71 viruses (rhabdomyosarcoma, RD cell,
China typical culture collection center (Wuhan University) (CCTCC) obtain) in be overexpressed structure pCMV-HERP expression matter
Grain, infected after 24 hours with a certain amount of EV71 viruses.The VP1 structural proteins and 3C non-structural proteins of EV71 viruses are as EV71
The detection mark of virus replication, and devise fluorogenic quantitative detection primer for VP1 GFPs.
C, time gradient is tested, and is transfected unloaded pCMV-Tag-Flag2B carriers and pCMV-HERP plasmids and is distinguished after 24 hours
Sample extraction total serum IgE is received after infection EV71 virus-4 hours, 8 hours, 12 hours and 24 hours and detects VP1 mRNA level in-site, simultaneously
It is horizontal to detect virus protein.
The above results show：The mRNA level in-sites of EV71 virus VP 1s can substantially be suppressed by being overexpressed HERP albumen, and with
The dose-dependent effect that gradient reduces is presented in the raising of HERP plasmid concentrations.Immunoblot experiment also show VP1 and 3C albumen
Expression reduced also with the raising of HERP expressing quantities.
The control group of uninfecting virus does not have significant difference, and infected virus and be overexpressed the experimental group of HERP albumen with
Zero load is compared, in the duplication for inhibiting virus that different time points is different degrees of.VP1 and 3C albumen then is have detected, and
The result similar with mRNA level in-site is obtained
Present invention finds the new medical value of the endoplasmic reticulum albumen of homocysteine induction, for anti-Enterovirus 71
Type, treatment and/or prevention EV71 infection provide new active drug.
The present invention compared with prior art, has advantages below and effect：
The anti-EV71 viruses of endoplasmic reticulum albumen (HERP) that the present invention is induced homocysteine from cell and molecular level
Effect is evaluated, and is had laid a good foundation for its further development and application.Show that the medicine has exploitation into anti-EV71
The active drug of virus and applied to clinical prospect.
Brief description of the drawings
Fig. 1 is the mRNA level in-site (* that a kind of HERP albumen suppresses EV71 virus VP 1s：p<0.5, significant difference；**：p<
0.05, difference is extremely notable) schematic diagram.
With the rise of pCMV-HERP expression vector transfection concentrations, the suppression of dose dependent is presented in EV71 mRNA level in-site
Effect processed.
Fig. 2 is that a kind of HERP albumen suppresses EV71 virus VP 1s and 3C protein expression schematic diagrames.
With the rise of pCMV-HERP expression vector transfection concentrations, dosage is presented in EV71 VP1 albumen and 3C protein levels
The inhibition of dependence.
Fig. 3 is that a kind of HERP albumen of different time gradient suppresses the mRNA level in-site (ns. of EV71 viruses：It is poor without conspicuousness
It is different；*：p<0.5, significant difference；**：p<0.05, difference is extremely notable) schematic diagram.
Under different viral infection times, with pCMV-HERP overexpression, EV71 mRNA level in-site is presented significantly
Inhibition.
Fig. 4 is that a kind of HERP albumen of different time gradient suppresses protein expression (the h p.i. of EV71 viruses:When after infection
Between) schematic diagram.
Under different viral infection times, with pCMV-HERP overexpression, EV71 VP1 albumen and 3C albumen water
It is flat that obvious inhibition is presented.
Embodiment
Following examples are used to further illustrate present disclosure, but should not be construed as limiting the invention, not
In the case of spirit of the invention and essence, the modifications or substitutions made to the inventive method, step or condition belong to this
The scope of invention.Unless otherwise specified, the conventional hand that technological means used in embodiment is well known to those skilled in the art
Section.
Embodiment 1：
A kind of preparation of the endoplasmic reticulum albumen plasmid vector of homocysteine induction, its step are：
A, endoplasmic reticulum albumen (HERP) genophore structure of homocysteine induction：
Clone the endoplasmic reticulum albumen gene of homocysteine induction：With HEK293T cell (China typical culture collections
Center (Wuhan University) (CCTCC) obtains) cDNA goes out HERP full-length gene orders as template PCR amplifications, first pass through reverse transcription
PCR expands total cDNA, then using the cDNA as template, complete HERP genetic fragments are amplified with special primer.Primer is as follows：
P1 (forward primer)：5 '-GAATTCATGGAGTCCGAGACCGAACCCGA-3 ', restriction enzyme site EcoRI；
P2 (reverse primer)：5 '-GTCGACTCAGTTTGCGATGGCTGGGGGG-3 ', restriction enzyme site SalI.
HERP gene clonings are connected to pCMV-Tag-Flag2B plasmids and carried by EcoRI and SalI digestions, T4 ligases
On body (mammalian expression vector, Agilent Technologies), pCMV-HERP plasmids are obtained.
Embodiment 2：
A kind of application of endoplasmic reticulum albumen of homocysteine induction in anti-EV71 virus infection, its step are：
A, the effect of the anti-EV71 viruses of the endoplasmic reticulum albumen of the homocysteine induction of various concentrations：
10ml Tissue Culture Flasks cultivate the people pernicious embryonal rhabdomyosarcoma cell sensitive to EV71 viruses through 24h
When (rhabdomyosarcoma, RD cell) covers with individual layer (the hyclone culture of MEM culture mediums+10%), tipping nutrient solution, add
Enter the digestion of 1ml pancreatin, the culture medium for adding (volume ratio) hyclone of 12ml MEM culture mediums+10% reaches 6 hole steril cells
In culture plate, per hole 2ml, 37 DEG C, 5%CO are placed in212h is cultivated in cell culture incubator, cell growth is abandoned supernatant into individual layer.
The quality that addition is diluted with the MEM cell culture fluids of serum-free is respectively 0.1,1,1.5,2,3 μ g pCMV-HERP plasmids, is incubated
Infected after educating 24h with EV71 viruses, infection multiplicity (multiplicities of infection, MOI) receives sample after being 5,12h
Detection, detect EV71 viruses with real-time fluorescence quantitative PCR (Realtime PCR) and Western blotting (Western Blot) respectively
MRNA level in-site and protein level (for wherein Realtime PCR using GAPDH as internal reference, primer sequence is positive 5'-
AAGGCTGTGGGCAAGG, reverse 5'-TGGAGGAGTGGGTGTCG；VP1 primer sequences are positive 5'-
AATTGAGTTCCATAGGTG, reverse 5'-CTGTGCGAATTAAGGACAG；EV71 VP1 antibody is purchased from Abnova companies, and 3C resists
Body is purchased from Abgent companies, and HERP antibody is purchased from Abcam companies, and GAPDH antibody is purchased from three hawk companies), such as Fig. 1 and Fig. 2, cross table
The mRNA level in-site of EV71 virus VP 1s can substantially be suppressed up to HERP albumen, and ladder is presented with the raising of HERP plasmid concentrations
Spend the dose-dependent effect (Fig. 1) reduced.Immunoblot experiment also show the expression of the VP1 and 3C albumen of EV71 viruses
Reduced (Fig. 2) also with the raising of HERP expressing quantities.The mRNA level in-site and protein level gradient of EV71 virus proteins
Decline, illustrating the endoplasmic reticulum albumen of the homocysteine induction of various concentrations has the function that anti-EV71 viruses.
B, the effect of the anti-EV71 viruses of endoplasmic reticulum albumen of the homocysteine induction of different time is handled：
10ml Tissue Culture Flasks cultivate (MEM when the pernicious embryonal rhabdomyosarcoma cell of people (RD cells) covers with individual layer through 24h
The culture of the hyclone of culture medium+10%), tipping nutrient solution, the digestion of 1ml pancreatin is added, adds 12ml MEM culture mediums+10%
The culture medium of (volume ratio) hyclone is reached in 6 hole steril cell culture plates, per hole 2ml, is placed in 37 DEG C, 5%CO2Cell is trained
Support in case and cultivate 12h, cell growth is abandoned supernatant into individual layer.It is separately added into the matter diluted with the MEM cell culture fluids of serum-free
Measure the control empty plasmid and pCMV-HERP plasmids for 2 μ g, be incubated after 24h with EV71 viruses infect respectively 4 hours, 8 hours,
12 hours and 24 hours, viral infection multiplicity was 5, and EV71 is detected with Realtime PCR and Western Blot respectively after receiving sample
The mRNA level in-site and protein level of virus, such as Fig. 3 and Fig. 4, the control group of uninfecting virus does not have significant difference, and has infected disease
Poison is simultaneously overexpressed the experimental group of HERP albumen compared with zero load, different time points it is different degrees of inhibit answering for virus
Make (Fig. 3).Applicant then have detected VP1 and 3C protein expression levels, and obtain the result similar with mRNA level in-site (figure
4).These results show that the endoplasmic reticulum albumen of the homocysteine induction after different time processing has the work of anti-EV71 viruses
With.
A kind of endoplasmic reticulum albumen of 3 homocysteine of embodiment induction is preparing treatment or prevention EV71 infection medicines
In application, its step is：
A, therapeutic action of the endoplasmic reticulum albumen of homocysteine induction to EV71 virus infection
(MEM culture mediums+10% when covering with individual layer through the 24-48h culture pernicious embryonal rhabdomyosarcoma cells of people (RD cells)
Hyclone culture), tipping nutrient solution, pancreatin digestion is added, is reached in 96 hole steril cell culture plates, per the μ L of hole 100, is placed in
37 DEG C, 5%CO218-24h is cultivated in cell culture incubator, cell growth is abandoned supernatant into individual layer.The MEM of addition serum-free is thin
The virus of born of the same parents' nutrient solution dilution, infective dose MOI=5, per the μ L of hole 100, it is incubated abandoning supernatant after 1h.By HERP albumen point
It is not transfected into 0.25,0.5,1,1.5 μ g/ml concentration in cell, while sets up cell control well and (be not added with viral not dosing, only
Add nutrient solution) and virus control wells (not dosing, adding virus, add nutrient solution).It is placed in 37 DEG C, 5%CO2Cultivated in cell culture incubator
24h, add 20 μ L/ hole CellTiterAQueous One Solution Reagent continue to be incubated 4h, terminate culture.
490nm wavelength is selected, each hole absorbance value is determined on enzyme linked immunological monitor, records result, and cell is calculated according to formula
Survival rate：Cell survival rate (%)=(HERP treatment groups OD values-virus control group OD values)/(cell controls group OD values-virus
Control group OD values) × 100%.It the results are shown in Table 1.
Therapeutic actions of the HERP of table 1 to EV71 virus infection
As a result show, the endoplasmic reticulum albumen of homocysteine induction, its survival rate are added after cell infection EV71 viruses
Apparently higher than virus control group, and as the rise of HERP albumen transfection concentrations, its viral suppression also gradually rise, shown
The endoplasmic reticulum albumen of homocysteine induction has preferable therapeutic action to EV71 viruses.
B, prevention effect of the endoplasmic reticulum albumen of homocysteine induction to EV71 virus infection
(MEM culture mediums+10% when covering with individual layer through the 24-48h culture pernicious embryonal rhabdomyosarcoma cells of people (RD cells)
Hyclone culture), tipping nutrient solution, pancreatin digestion is added, is reached in 96 hole steril cell culture plates, per the μ L of hole 100, is placed in
37 DEG C, 5%CO218-24h is cultivated in cell culture incubator, cell growth is abandoned supernatant into individual layer.By final concentration of 1 μ g/ml's
HERP plasmids distinguish transfectional cell 4,8,12 and 24h, and set up cell control well (be not added with viral not dosing, only plus nutrient solution) and
Virus control wells (not dosing, it is MOI=5 to add viral infective dose, adds nutrient solution).HERP plasmid transfections reach the above-mentioned time
Afterwards, supernatant discarding, addition serum-free MEM cell culture fluids dilution virus, infective dose MOI=5, be placed in 37 DEG C,
5%CO2Cell culture incubator culture 24h, add 20 μ L/ hole CellTiterAQueous One Solution Reagent after
It is continuous to be incubated 4h, terminate culture.490nm wavelength is selected, each hole absorbance value is determined on enzyme linked immunological monitor, records result,
And cell survival rate is calculated according to formula：Cell survival rate (%)=(HERP treatment groups OD values-virus control group OD values)/(thin
Born of the same parents' control group OD values-virus control group OD values) × 100%.It the results are shown in Table 2.
Prevention effects of the HERP of table 2 to EV71 virus infection
As a result showing, cell is after the endoplasmic reticulum albumen that homocysteine induces transfects in advance, then is infected, its
Survival rate is apparently higher than virus control group, and gradually rising with HERP transfection times, and the prevention of the infection to EV71 is made
With work more obvious, that the endoplasmic reticulum albumen that above-mentioned data display homocysteine induces infects with prevention EV71 viruses
With.
Claims (1)
1. application of the endoplasmic reticulum albumen of homocysteine induction in the medicine for treating or preventing EV71 infection is prepared.
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Non-Patent Citations (4)
| Title |
|---|
| herp, a new ubiquitin-like membrane protein induced by endoplasmic reticulum stress;Koichi Kokame等;《the journal of biological chemistry》;20001020;32846-32853 * |
| how viruses hijack the ERAD tuning machinery;julia noack等;《Journal of virology》;20140930;10272-10275 * |
| 多聚C结合蛋白-1与EV-71的5"非翻译区RNA相互作用在细胞内膜相关的复制复合体上促进病毒翻译与复制;罗震等;《湖北省暨武汉微生物学会会员代表大会暨学术年会》;20131231;全文 * |
| 肠道病毒71型感染对细胞内钙离子分布的影响;田洪超;《生物学杂志》;20141231;27-30 * |
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