CN105031043A - High-purity extraction method of tea polyphenol - Google Patents
High-purity extraction method of tea polyphenol Download PDFInfo
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- CN105031043A CN105031043A CN201510410932.8A CN201510410932A CN105031043A CN 105031043 A CN105031043 A CN 105031043A CN 201510410932 A CN201510410932 A CN 201510410932A CN 105031043 A CN105031043 A CN 105031043A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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Abstract
The invention relates to the technical field of beverages, in particular to a high-purity extraction method of tea polyphenol. The high-purity extraction method includes: through matched using of technologies of solvent leaching, precipitation, extraction and filtering, extracting a coarse product of the tea polyphenol; purifying the coarse product to obtain a pure product. Content of the tea polyphenol in the pure product is higher than 96%, the problem that the tea polyphenol is low in production efficiency and low in purity in the prior art is solved, and production cost of the tea polyphenol is lowered. The high-purity extraction method is more environment friendly and conforms to a concept of sustainable development.
Description
Technical field
The present invention relates to natural product extractive technique field, be specifically related to a kind of tea polyphenols method for extracting high purity.
Background technology
Tea polyphenols (TeaPolyphenols) is the general name of Polyphenols of Tea, comprises flavanol compound, anthocyanin class, flavonoid, flavonols and phenolic acids etc.Be mainly flavonol (catechin) class, catechin accounts for 60% ~ 80%.Class material tea polyphenols is tanned or tea tannin also known as tea, and being one of Main Ingredients and Appearance forming Tea color fragrance, is also one of Main Ingredients and Appearance having health care in Folium Camelliae sinensis.Research shows, tea polyphenols contains multiple phenolic hydroxyl group therefore tool removing toxic substances and radiation resistance, radioactive substance effectively can be stoped to invade bone marrow, and strontium 90 and Co 60 can be made to excrete rapidly, be described as " radiation jinx " by healthy and medical circle.Tea polyphenols is used widely in every field as a kind of additive, within 1989, is listed in GB2760-89 food additive by Food Additives Used in China association and uses standard, within 1997, be classified as Chinese patent medicine raw material.
Tea polyphenols has very strong antitumor action.Tea polyphenols (mainly catechin compounds) in Folium Camelliae sinensis can block the multiple carcinogen in vivo synthesis such as nitrite, and there is effect of direct killing cancerous cell and raising body immunity, to prevention and the auxiliary treatment of the kinds cancer such as gastric cancer, intestinal cancer, all helpful.
But in existing process for extracting tea polyphenol, often cost is higher, and have employed poisonous, the volatile organic solvent such as chloroform, production efficiency low-purity is lower, easily pollutes, unfriendly for environment.
Summary of the invention
The object of the present invention is to provide a kind of tea polyphenols method for extracting high purity, overcome the problem that in background technology, tea polyphenols production efficiency low-purity is lower, reduce the production cost of tea polyphenols, and environmental protection more, meet the spirit of sustainable development.
For realizing the object of the invention, the technical scheme of employing is:
A kind of tea polyphenols method for extracting high purity, is characterized in that comprising the following steps:
Prepare tea polyphenols crude extract:
A1. dry in the sun: Folium Camelliae sinensis is paved and carries out dry in the sun, dry in the sun thickness 1 ~ 3cm, dry in the sun 1 ~ 3 day, dry in the sun temperature controls at 20 ~ 24 degree;
A2. enzyme denaturing: Folium Camelliae sinensis after dry in the sun is carried out steaming and decocting, steaming and decocting 15 ~ 20min, temperature controls at 90 ~ 95 DEG C, suppresses and deactivation residual enzyme;
A3. slightly carry: joined by Folium Camelliae sinensis in ethyl acetate solvent, pH controls 2.5 ~ 3.5, reflux 2.5 ~ 3h, sucking filtration obtains lixiviating solution, and join in liquor zinci chloridi after lixiviating solution is concentrated, adjust ph is to 7, carry out Precipitation adding sodium chloride, precipitation is carried out sucking filtration and obtains filter cake;
A4. extract: joined by filter cake in hydrochloric acid solution, stirring and dissolving, pH controls 4 ~ 4.5, and be heated to micro-filtration of boiling, filtrate adds extraction into ethyl acetate three times, and combining extraction liquid concentrates and obtain tea polyphenols crude extract;
Prepare tea polyphenols sterling:
B1. dissolve: tea polyphenols crude extract is joined in sodium chloride solution, pH regulator to 7;
B2. centrifugal: centrifugal segregation particulate contamination;
B3. fine straining: employing aperture is that the melt-blown filter of 5 μm filters the tea polyphenols solution after centrifugal;
B4. ultrafiltration membrance filter: adopt ultrafilter membrane to filter the solution after centrifugal;
B5. extract: again by the filtrate ether extraction after fine straining, namely lyophilization obtains sterling.
Preferably, the rotating speed during work of step B2 centrifuge is 5000 ~ 5400r/min.
Preferably, during step B4 ultrafiltration membrance filter, the temperature of filtrate is 40 ± 2 DEG C.
By adopting above-mentioned technical scheme, the invention has the beneficial effects as follows: the present invention by the technology such as extraction, precipitation, extraction, filtration with the use of, in the crude product obtained after tea polyphenols crude extract evaporate to dryness, polyphenol content is more than 75%, again through purification step, in sterling, polyphenol content is more than 96%, can save production cost in a large number, and do not use toxicity higher and volatile solvent, substantially increase the safety of existence and product.Therefore, the present invention has complied with the tendency of the day of ecology, environmental protection, is industry exploitation safety, efficient, low cost tea polyphenols method for extracting high purity, reduces one of active approaches of environmental pollution.
Detailed description of the invention
Be described in further detail below by specific embodiment.
Prepare tea polyphenols crude extract:
A1. dry in the sun: Folium Camelliae sinensis is paved and carries out dry in the sun, dry in the sun thickness 1 ~ 3cm, dry in the sun 1 ~ 3 day, dry in the sun temperature controls at 20 ~ 24 degree;
A2. enzyme denaturing: Folium Camelliae sinensis after dry in the sun is carried out steaming and decocting, steaming and decocting 15 ~ 20min, temperature controls at 90 ~ 95 DEG C, suppresses and deactivation residual enzyme;
A3. slightly carry: joined by Folium Camelliae sinensis in ethyl acetate solvent, pH controls 2.5 ~ 3.5, reflux 2.5 ~ 3h, sucking filtration obtains lixiviating solution, and join in liquor zinci chloridi after lixiviating solution is concentrated, adjust ph is to 7, carry out Precipitation adding sodium chloride, precipitation is carried out sucking filtration and obtains filter cake;
A4. extract: joined by filter cake in hydrochloric acid solution, stirring and dissolving, pH controls 4 ~ 4.5, and be heated to micro-filtration of boiling, filtrate adds extraction into ethyl acetate three times, and combining extraction liquid concentrates and obtain tea polyphenols crude extract;
Prepare tea polyphenols sterling:
B1. dissolve: tea polyphenols crude extract is joined in sodium chloride solution, pH regulator to 7;
B2. centrifugal: centrifugal segregation particulate contamination;
B3. fine straining: adopt ultrafilter membrane to filter the solution after centrifugal;
B4. ultrafiltration membrance filter: adopt ultrafilter membrane to filter the solution after centrifugal;
B5. extract: again by the filtrate ether extraction after fine straining, namely lyophilization obtains sterling.
Preferably, the rotating speed during work of step B2 centrifuge is 5000 ~ 5400r/min.
Preferably, during step B4 ultrafiltration membrance filter, the temperature of filtrate is 40 ± 2 DEG C.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. a tea polyphenols method for extracting high purity, is characterized in that comprising the following steps:
Prepare tea polyphenols crude extract:
A1. dry in the sun: Folium Camelliae sinensis is paved and carries out dry in the sun, dry in the sun thickness 1 ~ 3cm, dry in the sun 1 ~ 3 day, dry in the sun temperature controls at 20 ~ 24 degree;
A2. enzyme denaturing: Folium Camelliae sinensis after dry in the sun is carried out steaming and decocting, steaming and decocting 15 ~ 20min, temperature controls at 90 ~ 95 DEG C, suppresses and deactivation residual enzyme;
A3. slightly carry: joined by Folium Camelliae sinensis in ethyl acetate solvent, pH controls 2.5 ~ 3.5, reflux 2.5 ~ 3h, sucking filtration obtains lixiviating solution, and join in liquor zinci chloridi after lixiviating solution is concentrated, adjust ph is to 7, carry out Precipitation adding sodium chloride, precipitation is carried out sucking filtration and obtains filter cake;
A4. extract: joined by filter cake in hydrochloric acid solution, stirring and dissolving, pH controls 4 ~ 4.5, and be heated to micro-filtration of boiling, filtrate adds extraction into ethyl acetate three times, and combining extraction liquid concentrates and obtain tea polyphenols crude extract;
Prepare tea polyphenols sterling:
B1. dissolve: tea polyphenols crude extract is joined in sodium chloride solution, pH regulator to 7;
B2. centrifugal: centrifugal segregation particulate contamination;
B3. fine straining: employing aperture is that the melt-blown filter of 5 μm filters the tea polyphenols solution after centrifugal;
B4. ultrafiltration membrance filter: adopt ultrafilter membrane to filter the solution after centrifugal;
B5. extract: again by the filtrate ether extraction after fine straining, namely lyophilization obtains sterling.
2. a kind of tea polyphenols method for extracting high purity according to claim 1, is characterized in that: the rotating speed during work of step B2 centrifuge is 5000 ~ 5400r/min.
3. a kind of tea polyphenols method for extracting high purity according to claim 1, is characterized in that: during step B4 ultrafiltration membrance filter, and the temperature of filtrate is 40 ± 2 DEG C.
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| CN201510410932.8A CN105031043A (en) | 2015-07-14 | 2015-07-14 | High-purity extraction method of tea polyphenol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943768A (en) * | 2017-04-01 | 2017-07-14 | 北京国康本草物种生物科学技术研究院有限公司 | Tea Polyphenols and its extracting method and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943768A (en) * | 2017-04-01 | 2017-07-14 | 北京国康本草物种生物科学技术研究院有限公司 | Tea Polyphenols and its extracting method and application |
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