CN105030875A - Preparation method of honey-fried codonopsis pilosula medicinal slices - Google Patents

Abstract

The invention discloses a preparation method of honey-fried codonopsis pilosula medicinal slices. The preparation method of the honey-fried codonopsis pilosula medicinal slices comprises the following steps of (1) drying fresh codonopsis pilosula medicinal materials at the temperature of 70-90 DEG C until the water content of the codonopsis pilosula medicinal materials reaches 30%-70% and cutting off the dried codonopsis pilosula medicinal materials to obtain fresh codonopsis pilosula slices; (2) moistening the fresh codonopsis pilosula slices in processed honey for 2-24 hours under the condition that the mass ratio of the processed honey to the fresh codonopsis pilosula slices is (1-5): 10; (3) paving the moistened codonopsis pilosula slices, heating the codonopsis pilosula slices in a microwave manner and stirring the codonopsis pilosula slices at time intervals of 1 minute; and (4) drying the slices which are heated in the microwave manner until the slices are dry, taking out the slices and airing the slices at room temperature so as to obtain the honey-fried codonopsis pilosula medicinal slices. Compared with the prior art, the preparation method has the advantages that the medicinal materials are cut off easily and the thicknesses of the slices can be uniform under the condition of the adjusted water content, and the danger of mildewing and deterioration of the medicinal materials can be avoided.

Description

A kind of preparation method of processed with honey Radix Codonopsis decoction pieces

Technical field

The present invention relates to the concocting method field of Chinese medicine, be specifically related to a kind of preparation method of processed with honey Radix Codonopsis decoction pieces.

Background technology

The quality of the prepared slices of Chinese crude drugs directly has influence on the clinical efficacy of Chinese medicine.The concocting process of decoction pieces is all from medical material for a long time, through the process only making, moisten system, cutting, big gun are processed.And before medical material cutting, usually needing to carry out sofening treatment, softening process then can cause the loss of decoction pieces effective ingredient to some extent.If at source by the medical material of fresh collection by namely cutting into slices after suitable dry in the sun, not only can reduce the loss of effective ingredient, simultaneously owing to decreasing middle storage, transit link, also can avoid because haul-cycle time and preserve the improper About The Quality of Sliced Herbal Medicine caused and decline and loss.Can reduce the input of decoction pieces manufacturing enterprise in equipment, manpower and the energy in whole process, the final production cost reducing the prepared slices of Chinese crude drugs, promotes the quality of decoction pieces.

Radix Codonopsis is the dry root of Campanulaceae Radix Codonopsis Codonopsispilosula (Franch.) Nannf., element flower Radix Codonopsis CodonopsispilosulaNannf.var.modesta (Nannf.) L.T.Shen or radix codonpsis tangshen CodonopsistangshenOliv..Property is put down, and sweet in the mouth is conventional Chinese medicine.Traditional Chinese medical theory thinks raw Radix Codonopsis invigorating the spleen and replenishing QI, invigorating the spleen to benefit the lung, and the raw product of Radix Codonopsis are not identical with effect of goods Radix Codonopsis, emphasize particularly on different fields, clinically during use cure or parching to brown multiplex with processed product.Refer to processed with honey Radix Codonopsis in " controlling gold " clearly, the tight western thing of the Qing Dynasty mentions Radix Codonopsis " tonifying the lung, honey is mixed and cooked " in " must join book on Chinese herbal medicine ".

Traditional processed with honey process is the every 100kg of Radix Codonopsis decoction pieces, uses refined honey 25kg, and get the appropriate cold boiled water dilution of refined honey, mix thoroughly with Radix Codonopsis, slightly vexed, after mulse exhausts, drop in hot pot, heating is stirred, and to Radix Codonopsis in golden yellow, not too takes out during sticky hands and cools.Whole process takes time and effort, and temperature and time etc. processed by the not clear and definite fire of technological parameter, and due to the selected quality directly affecting Radix Codonopsis processed with honey product of technological parameter, therefore the processing procedure of specification processed with honey Radix Codonopsis decoction pieces is most important; Moistening time also more difficult grasp in processed with honey Radix Codonopsis process in addition, by the impact of above-mentioned factor, the stability of processed with honey quality is often criticized in very difficult guarantee.

Summary of the invention

Object of the present invention is exactly for above-mentioned defect of the prior art, provides a kind of preparation method of processed with honey Radix Codonopsis decoction pieces.

To achieve these goals, technical scheme provided by the invention is: a kind of preparation method of processed with honey Radix Codonopsis decoction pieces, comprises the following steps:

1) get fresh codonopsis pilosula, divide into the medical material layer of 5-10cm thickness, be placed in baking oven, be dried to water content 30%-70% with 70-90 DEG C, period is stirred 1-2 time, then is cut into the disk of 5-10mm, obtains Radix Codonopsis fresh slices;

2) by step 1) the Radix Codonopsis fresh slices that obtains is placed in refined honey moistening, and the mass ratio of refined honey and Radix Codonopsis fresh slices is (1-5): 10, moistening 2-24h, obtains Radix Codonopsis section after moistening;

3) by step 2) after the moistening that obtains Radix Codonopsis section divide and be suitable in container in microwave, heat 1-5min with microwave power 500-1000W, stir once every 1min, obtain microwave heating section;

4) by step 3) section of the microwave heating that obtains is placed in iron pan, and with 2100W electromagnetic oven, the paramount fire of moderate heat is fried to dry, takes out, dries in the air to room temperature, and sieve removes chip, vacuum packaging, namely obtains processed with honey Radix Codonopsis decoction pieces.

Further, the preparation method of above-mentioned a kind of processed with honey Radix Codonopsis decoction pieces,

Described step 1) in, bake out temperature is 70-80 DEG C, and being dried to water content is 40%-50%, and period stirs 2 times;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 1:10, and the moistening time is 4h;

Described step 3) in, microwave power is 700W, and heat time heating time is 2-4min.

Further, the preparation method of above-mentioned a kind of processed with honey Radix Codonopsis decoction pieces,

Described step 1) in, bake out temperature is 70-80 DEG C, and being dried to water content is 50%-60%, and period stirs 1 time;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, and the moistening time is 4-10h;

Described step 3) in, microwave power is 700W, and heat time heating time is 2-4min.

Further, the preparation method of above-mentioned a kind of processed with honey Radix Codonopsis decoction pieces,

Described step 1) in, bake out temperature is 70-90 DEG C, and being dried to water content is 60%-70%, and period stirs 1 time;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 1:10, and the moistening time is 4-24h;

Described step 3) in, microwave power is 700W, and heat time heating time is 2-4min.

Further, the preparation method of above-mentioned a kind of processed with honey Radix Codonopsis decoction pieces, described step 1) in, bake out temperature is 70-90 DEG C, and being dried to water content is 40%-70%.

Further, the preparation method of above-mentioned a kind of processed with honey Radix Codonopsis decoction pieces,

Described step 1) in, being dried to water content is 50%;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, and the moistening time is 8h;

Described step 3) in, microwave power is 700W, and heat time heating time is 2-4min.

Beneficial effect of the present invention is: the preparation method of a kind of processed with honey Radix Codonopsis decoction pieces provided by the invention, compared with prior art, fresh codonopsis pilosula first adopt baking oven 70-90 DEG C smoke to water content be 30%-70%, take out section, on the one hand under this water content, medical material cutting easily and the easy control strip of medical material sheet is evenly thick, decreases natural drying process on the other hand because of reasons such as weather and causes the danger of medical material moldy metamorphism.The concrete processing procedure parameter of the processed with honey Radix Codonopsis provided, comprises the addition of refined honey, furnace drying method, the heating power of microwave power during oven dry, time and electromagnetic oven; Core procedure is that medical material 70-90 DEG C smokes and carries out cutting to during water content 50%, and now medical material cutting is comparatively easy, allows medical material cut into slices simultaneously and fully contact with refined honey before processed with honey, to reach the abundant moistening that medical material is cut into slices.Modern dry technology, as the use of microwave drying and electromagnetic heating technique, greatly reduces operating procedure and time, more efficient, and achieves the quantitatively controlled of process.Meanwhile, the assay condition of the present invention foundation lobetyolin and atractylenoide Ⅲ, by investigating the content of Radix Codonopsis polysaccharide in processed with honey Radix Codonopsis, Radix Codonopsis total flavones, lobetyolin and atractylenoide Ⅲ, draw the best processing procedure of Radix Codonopsis, standardization for Radix Codonopsis is produced and is provided theoretical foundation, is beneficial to the quality controlling Radix Codonopsis decoction pieces.

Detailed description of the invention

Embodiment 1:

The processing procedure of Radix Codonopsis can be reduced to five steps: fresh medical material is smoked, cut into slices, add refined honey moistening, microwave drying, electromagnetic oven parch, cooling.

In above-mentioned six steps, need the technological parameter determined to be respectively: when fresh medical material baked temperature, section medical material moisture, add refined honey moistening time, the refined honey amount added, microwave time, electromagnetic oven parch.

For determining the design parameter of above-mentioned technique, do following several groups of experiments:

1, the preparation of sample:

1.1 by the preparation of the inventive method processed with honey Radix Codonopsis sample:

Sample S1-S8 processed with honey method:

Get fresh codonopsis pilosula 8 parts, divide into the thick medical material layer of 5-10cm respectively, put 80 DEG C, baking oven and be dried to water content and be respectively 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, period turns over 1 time, is cut into the disk of 5-10mm.Moistening in refined honey, the mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, moistening 8h, then the section of the Radix Codonopsis of moistening in refined honey is divided and is placed in the applicable disk of microwave or stainless steel disc.Microwave power is that 700W heats 4min, stirs once every 1min.After microwave heating, mixture is put in iron pan, and 2100W electromagnetic oven height fire is fried to dry.Take out, dry in the air to room temperature.Sieve removes chip, vacuum packaging, obtains processed with honey Radix Codonopsis decoction pieces.

Sample S9-S16 processed with honey method:

Get fresh codonopsis pilosula 8 parts, divide into the thick medical material layer of 5-10cm respectively, put baking oven respectively at 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C, 100 DEG C, 110 DEG C, 120 DEG C are dried to water content and are respectively 50%, and period turns over 1 time, is cut into the disk of 5-10mm.Moistening in refined honey, the mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, moistening 8h, then the section of the Radix Codonopsis of moistening in refined honey is divided and is placed in the applicable disk of microwave or stainless steel disc.Microwave power is that 700W heats 4min, stirs once every 1min.After microwave heating, mixture is put in iron pan, and 2100W electromagnetic oven height fire is fried to dry.Take out, dry in the air to room temperature.Sieve removes chip, vacuum packaging, obtains processed with honey Radix Codonopsis decoction pieces.

The preparation of 1.2 traditional processed with honey Radix Codonopsis decoction pieces:

The every 100kg of Radix Codonopsis decoction pieces, uses refined honey 25kg, and get the appropriate cold boiled water dilution of refined honey, mix thoroughly with Radix Codonopsis, slightly vexed, after mulse exhausts, drop in hot pot, heating is stirred, and to Radix Codonopsis in golden yellow, not too takes out during sticky hands, cools and obtain traditional processed with honey Radix Codonopsis decoction pieces.

2, inspection target assay method:

The mensuration of 2.1 lobetyolin's content:

2.1.1 chromatographic condition:

Chromatographic column DiamonsilC18 (250 × 4.6mm, 5 μm).Take acetonitrile as mobile phase A, with 0.2% acetic acid for Mobile phase B, carry out gradient elution by regulation in table 1; Column temperature 30 DEG C, determined wavelength 267nm.Table 1 is shown as elution requirement.

Table 1

Time (min)	Mobile phase (A) (%)	Mobile phase (B) (%)
			0-20	10→20	90→80
20-60	20→100	80→0

2.1.2 the preparation of need testing solution:

Get Radix Codonopsis decoction pieces pulverize and cross 20 mesh sieves, accurately weighed 2.0g, put in round-bottomed flask, add 10, the methanol of 8 times amount, reflux, extract, 2 times, each 40min, filter, get subsequent filtrate and be concentrated to 10mL.Shake up standing, get supernatant, filter with microporous filter membrane (0.45 μm), get subsequent filtrate, obtain each technique need testing solution.

2.1.3 the preparation of standard solution:

Take 1.18mg lobetyolin in the volumetric flask of 2mL, concentration is 0.590mg/mL.

2.1.4 measure:

The above-mentioned reference substance of accurate absorption and need testing solution 10 μ L respectively, injection liquid chromatography, measures, to obtain final product.

The mensuration of 2.2 atractylenoide Ⅲ content:

2.2.1 chromatographic condition:

Chromatographic column DiamonsilC18 (250 × 4.6mm, 5 μm).Being mobile phase A with acetonitrile, take water as Mobile phase B, A:B (71:29); Flow velocity: 1mL/min, column temperature 30 DEG C, determined wavelength 220nm, sample size 10 μ l.

2.2.2 the preparation of need testing solution:

Get Radix Codonopsis decoction pieces pulverize and cross 20 mesh sieves, accurately weighed 2.0g, put in round-bottomed flask, add 10, the methanol of 8 times amount, reflux, extract, 2 times, each 40min, filter, get subsequent filtrate and be concentrated to 10mL.Shake up standing, get supernatant, filter with microporous filter membrane (0.45 μm), get subsequent filtrate, obtain each technique need testing solution.

2.2.3 the preparation of standard solution:

Take 1.97mg standard substance in the volumetric flask of 2mL, concentration is 0.985mg/mL.

2.2.4 measure:

The above-mentioned reference substance of accurate absorption and need testing solution 10 μ L respectively, injection liquid chromatography, measures, to obtain final product.

The foundation of 2.3 finger printing:

2.3.1 chromatographic condition:

Chromatographic column DiamonsilC18 (250 × 4.6mm, 5 μm).Take acetonitrile as mobile phase A, with 0.2% acetic acid water for Mobile phase B, gradient elution, elution requirement in table 2, flow velocity: 1ml/min, column temperature 30 DEG C, determined wavelength 267nm, sample size 10 μ l.Chromatographic elution conditions is as shown in table 2.

Table 2

Time (min)	Mobile phase (A) (%)	Mobile phase (B) (%)
			0-15	10→20	90→80
15-30	20	80
			30-40	20→30	80→70
40-55	30→45	70→55
			55-70	45→100	55→0

2.3.2 the preparation of need testing solution:

Processed with honey Radix Codonopsis is pulverized, and cross 40 mesh sieves, accurately weighed 2.00g, puts in the conical flask of 250ml, and precision adds 45% ethanol 100ml, jam-pack, weighed weight, leaves standstill after 1 hour, connects reflux condensing tube, is heated to boiling, and keeps micro-ly boiling for 1.5 hours.Conical flask is taken off, close plug after letting cool, weighed, supply the weight of less loss with 45% ethanol, shake up, filter with dry filter, obtain ethanol extract.

Precision measures 75ml ethanol extract, after concentrated by rotary evaporation to 5ml, by macroporous adsorbent resin (HPD-100), first use 100ml distilled water eluting, discard water liquid, then with 150ml, 50% ethanol elution, collect ethanol elution, by eluent evaporate to dryness, by chromatograph methanol constant volume to 2ml, filter with microporous filter membrane (0.45 μm), get subsequent filtrate, obtain each technique need testing solution.

2.3.3 methodological study:

2.3.3.1 withinday precision is tested:

Accurate absorption same sample solution, repeat sample introduction 5 times, each 10 μ l, be with reference to peak with lobetyolin, the relative retention time of each main peaks and relative peak area in calculated fingerprint collection of illustrative plates, the RSD of the relative retention time of each chromatographic peak of result is 0.117%, and the RSD of relative peak area is 1.101%, shows that instrument precision is good.

2.3.3.2 day to day precision is tested:

Accurate absorption same sample solution, continuous five day every day sample introduction 1 time, each sample size is 10 μ l, be with reference to peak with lobetyolin, the relative retention time of each main peaks and relative peak area in calculated fingerprint collection of illustrative plates, the RSD of the relative retention time of each chromatographic peak of result is 0.099%, and the RSD of relative peak area is 1.125%, show that instrument precision is good, meet the requirements.

2.3.3.3 stability test:

Accurate absorption same sample solution, ambient temperatare is put, respectively 0,12,24,48h sample introduction 10 μ l measures, the RSD of the relative retention time at each main total peak of result is 0.892%, and the RSD of relative peak area is 1.135%, and sample solution is at room temperature more stable in 48h.

2.3.3.4 replica test:

By the method for 2.3.2, prepare the processed with honey Radix Codonopsis sample solution 6 parts of same lot number, according to above-mentioned condition sample introduction respectively, record finger printing, the RSD of the relative retention time at each main total peak of result is 0.961%, and the RSD of relative peak area is 1.457%, shows that this method repeatability is good.

2.3.3.5 the demarcation of total fingerprint peaks:

Have finger print region at the processed with honey Radix Codonopsis sample of 16 Different Preparation and demarcated 17 total peaks altogether, selected total peak area sum accounts for more than 90% of total peak area, comparatively comprehensively can reflect the inherent quality of sample.

Antioxidant activity research (DPPH) of each processing technique of the fresh medical material of 2.4 Radix Codonopsis:

2.4.1DPPH the configuration of methanol solution:

Precise gets 4mgDPPH, and with absolute methanol standardize solution in 100mL volumetric flask, obtaining concentration is 0.1mmolL ^-1dPPH solution, keep in Dark Place in brown bottle, matching while using.

2.4.2 the preparation of sample solution:

Method is with " 2.3.2 ".

2.4.3 sample determination:

2.4.3.1 sample liquid 100 μ L and 0.1mmolL is pipetted ^-1dPPH solution 2mL adds after the concussion of same test tube mesoscale eddies shakes up, and is placed in darkroom, accurate response 30min, surveys its absorbance As at 517nm wavelength, repeat to average for 3 times.

2.4.3.2 0.1mmolL is pipetted ^-1dPPH solution 2mL mixes with 100 μ L absolute methanol solutions, measures mixed absorbance A b.

2.4.3.2 pipette absolute methanol 2mL to mix with 100 μ L sample solutions, measure mixed absorbance A c.

According to formulae discovery clearance rate SR (Thepercentageofscavengingradicalcapacity) below.

SR(％)＝[1-(As-Ac)/Ab)]×100％；

Ab: the absorbance of blank;

As: the absorbance adding sample solution;

Ac: Background control;

Experiment application of sample table is as shown in table 3.

Table 3

	Sample solution	0.1mmol/LDPPH solution	Methanol solution
				As	100μL	2mL	0
Ab	0	2mL	100μL
				Ac	100μL	0	2mL

2.5 experimental results:

2.5.1S1-S16 the assay result (n=3) as shown in table 4 of lobetyolin and atractylenoide Ⅲ in number processed with honey Radix Codonopsis decoction pieces.

Table 4

Sample	Lobetyolin (mg/g)	Atractylenoide Ⅲ (μ g/g)
			Tradition sample	1.42±0.02	3.57±0.08
S1	1.11±0.04	3.70±0.11
			S2	1.38±0.05	10.88±0.70
S3	1.90±0.04	10.95±0.66
			S4	0.82±0.04	9.14±0.18
S5	1.01±0.04	10.64±0.47
			S6	0.91±0.04	4.27±0.13
S7	1.04±0.03	6.66±0.03
			S8	1.27±0.01	5.44±0.06
S9	0.67±0.04	2.10±0.07
			S10	0.95±0.03	9.20±0.06
S11	1.58±0.02	8.23±0.07
			S12	1.90±0.04	9.02±0.08
S13	2.01±0.04	4.20±0.07
			S14	1.94±0.04	3.89±0.13

S15	1.86±0.02	3.98±0.02
			S16	1.49±0.04	4.55±0.11

As shown in Table 4, in sample S3, lobetyolin and atractylenoide Ⅲ are all higher, and far away higher than traditional sample.The content of sample S11-S16 lobetyolin is all higher than traditional sample; S10-S16 atractylenoide Ⅲ content is far away higher than traditional sample.

2.5.2 fingerprint similarity analysis:

Adopt Chinese Pharmacopoeia Commission's similarity evaluation (2004A) to carry out data analysing method and set S1 as reference collection of illustrative plates, the chromatographic peak of other samples is carried out Auto-matching with reference to collection of illustrative plates, calculate S1 ~ S16 sample and the similarity contrasting collection of illustrative plates, it is as shown in the table to obtain the similarity of the ethanol extract finger printing of different modern processed mode.Between the Radix Codonopsis decoction pieces ethanol extraction of each processing method gained, similarity is higher, and all more than 0.930, the similarity of the alcohol leaching thing finger printing of Different treatments is as shown in table 5.To say objectively the primary chemical feature similarity of the codonopsis pilosula of each modern processed is understood.There is again difference definitely in the similarity carefully comparing each sample, illustrates that between different modern processed modes, chemical feature has certain difference.

Table 5

2.5.2 the results and analysis of finger printing:

The peak area at main total peak and the ratio of sample weighting amount (g) are in table 6, table 7 under 267nm to adopt different processed with honey Radix Codonopsis sample, and the processed with honey Radix Codonopsis prepared by the inventive method has the rate of change of peak-to-peak area and sample weighting amount to react the diversity between each technique relative to processed with honey Radix Codonopsis sample finger printing prepared by traditional method.Table 6 is shown as S1-S8 sample and has the ratio of peak-to-peak area and sample weighting amount (g) and the comparative result of traditional handicraft.Table 7 is shown as S9-S15 sample and has the ratio of peak-to-peak area and sample weighting amount (g) and the comparative result of traditional handicraft (TD).

Table 6

Total peak	S1	S2	S3	S4	S5	S6	S7	S8	TD
										P1	640.71	634.03	642.44	629.03	608.62	607.85	493.67	506.81	500.64
P2	183.08	125.67	150.85	154.51	204.82	247.35	224.59	156.98	156.86
										P3	617.96	565.65	764.18	109.58	321.35	579.83	217.24	321.88	481.52
P4	152.31	150.68	154.34	101.60	108.37	56.31	95.80	66.11	47.08
										P5	260.65	139.32	382.51	169.07	240.78	238.70	232.69	45.35	469.01
P6	199.98	185.62	203.01	182.30	205.73	137.02	120.83	123.26	149.21
										P7	345.56	279.49	439.57	455.48	459.52	437.79	499.44	390.54	359.59
P8	126.35	157.17	245.02	76.00	97.70	88.05	122.41	98.05	154.23
										P9	443.60	326.73	794.26	369.87	453.20	365.54	438.66	450.87	414.02
P10	178.60	183.03	160.97	148.60	178.23	203.76	293.73	262.01	112.86
										P11	125.25	78.68	149.70	117.61	123.55	81.83	101.44	123.99	58.06
P12	106.93	135.66	145.01	121.20	136.27	141.48	130.22	134.82	100.46
										P13	3486.9	4368.1	4298.8	3659.9	3714.3	3815.1	3568.9	3776.7	3433.9
P14	133.83	125.12	150.78	171.16	272.23	179.21	110.84	107.60	278.67
										P15	107.54	132.87	301.22	218.45	221.88	235.98	192.05	257.67	183.01
P16	302.02	278.34	379.75	297.44	418.34	438.86	378.21	529.15	409.17
										P17	169.10	156.00	279.95	264.27	111.54	100.84	123.53	126.21	65.25
On average	445.90	471.89	567.20	426.24	463.32	467.97	432.01	439.88	433.74

Table 7

Total peak	S9	S10	S11	S12	S13	S14	S15	S16	TD
										P1	657.04	571.33	619.33	642.44	438.76	916.41	931.05	1031.8	500.64
P2	158.31	216.29	199.51	150.85	153.33	527.53	313.27	237.58	156.86
										P3	464.17	442.51	783.51	764.18	738.28	1879.0	1895.4	963.12	481.52
P4	116.32	88.44	90.66	154.34	138.27	161.87	173.21	244.09	47.08
										P5	333.13	295.17	335.68	382.51	380.67	392.82	395.08	350.41	469.01
P6	206.64	173.05	154.99	203.01	180.91	209.44	199.71	200.02	149.21
										P7	409.37	458.53	431.34	439.57	370.20	745.72	945.21	550.70	359.59
P8	141.70	195.85	185.54	245.02	178.04	165.96	151.89	119.28	154.23
										P9	466.20	464.53	645.49	794.26	601.03	693.03	670.44	492.43	414.02
P10	188.77	204.43	501.88	160.97	189.49	190.00	205.64	163.40	112.86
										P11	190.64	167.28	170.18	149.70	228.67	209.16	256.53	137.02	58.06
P12	122.63	120.71	270.34	145.01	146.47	140.13	145.59	133.10	100.46
										P13	2792.5	2853.8	4480.7	4298.8	4198.3	4034.0	4378.9	4311.0	3433.9
P14	205.68	193.74	432.26	150.78	148.99	161.82	192.63	210.97	278.67
										P15	461.19	433.27	760.85	301.22	215.90	214.74	254.77	140.05	183.01

P16	332.09	301.55	345.69	379.75	255.00	285.34	282.89	202.66	409.17
										P17	211.59	158.14	115.64	279.95	198.06	200.66	164.32	114.88	65.25
On average	438.70	431.68	619.03	567.20	515.32	654.57	679.80	564.85	433.74

As shown in Table 6, total peak P2, P3, P5, P8, P9, P11, P13, P14, P15, P16 and No. P17, the peak area at the total peak at these 11 peaks and sample weighting amount ratio are to change of moisture content sensitivity.Wherein except No. 2 peaks the peak area at the total peak at other peaks and sample weighting amount ratio all with S3 the highest while being dried to water content 50% (80 DEG C cut into slices).And the peak area at the total peak at P2, P3 and No. P16 3 peaks and sample weighting amount ratio higher with traditional handicraft processing mode, all the other each peaks are all that the ratio of the total peak-to-peak area of the Radix Glycyrrhizae (processed with honey) that this inventive method obtains and sample weighting amount is higher.

As shown in Table 7, P1, P2, P4, P6, P7, P8, P9, P10, P15, P16, No. P17 11 peak have the peak area at peak and sample weighting amount ratio to the sensitive of temperature.Wherein except P1, P2, P7 and No. P10 4 peaks, the peak area at the total peak at all the other peaks and sample weighting amount ratio are all at S12 the highest while being dried to water content 50% (80 DEG C cut into slices).Except P16 peak has peak area and sample weighting amount ratio a little less than traditional handicraft, all the other are all higher than traditional handicraft.

2.6S1-S18 sample DPPH clearance rate result, as shown in table 8.

Table 8

Sample	DPPH clearance rate %
		Tradition sample	47.59±0.59
S1	48.01±0.23
		S2	54.90±0.82
S3	56.40±0.82
		S4	51.50±0.70
S5	53.07±0.35
		S6	51.91±0.12
S7	49.42±0.35
		S8	44.77±1.06
S9	44.10±0.35
		S10	46.59±0.35
S11	47.84±0.94
		S12	55.23±0.82
S13	56.06±0.82
		S14	58.55±0.35
S15	60.50±0.02
		S16	47.76±0.82

As shown in Table 8, the DPPH clearance rate of the processed with honey Radix Codonopsis prepared by the present invention compares with traditional processed with honey Radix Codonopsis sample, and except S9 and S10 two sample DPPH clearance rate are less than except traditional handicraft, the DPPH clearance rate of all the other samples is all better than traditional processed with honey Radix Codonopsis sample.And it is the highest with the DPPH clearance rate of S3 (56.40%) and S2 (55.23).

Consider, select 80 DEG C of baking ovens to smoke fresh codonopsis pilosula to medical material water content 50%, section.The mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, and moistening 8h, 700W microwave heating 4min, stirs once every 1min.After microwave heating, the paramount fire of 2100W electromagnetic oven moderate heat is fried to dry, as best Honey processing technology.Illustrate that method of the present invention is compared with traditional method, there is the loss reducing effective ingredient, strengthen the effect of oxidation resistance.

Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (6)

1. a preparation method for processed with honey Radix Codonopsis decoction pieces, is characterized in that, comprises the following steps:

1) get fresh codonopsis pilosula, divide into the medical material layer of 5-10cm thickness, be placed in baking oven, be dried to water content 30%-70% with 70-90 DEG C, period is stirred 1-2 time, then is cut into the disk of 5-10mm, obtains Radix Codonopsis fresh slices;

2) Radix Codonopsis fresh slices step 1) obtained is placed in refined honey moistening, and the mass ratio of refined honey and Radix Codonopsis fresh slices is (1-5): 10, moistening 2-24h, and after obtaining moistening, Radix Codonopsis is cut into slices;

3) by step 2) after the moistening that obtains Radix Codonopsis section divide and be suitable in container in microwave, heat 1-5min with microwave power 500-1000W, stir once every 1min, obtain microwave heating section;

4) microwave heating section step 3) obtained is placed in iron pan, and with 2100W electromagnetic oven, the paramount fire of moderate heat is fried to dry, takes out, dries in the air to room temperature, and sieve removes chip, vacuum packaging, namely obtains processed with honey Radix Codonopsis decoction pieces.

2. the preparation method of a kind of processed with honey Radix Codonopsis decoction pieces according to claim 1, is characterized in that,

In described step 1), bake out temperature is 70-80 DEG C, and being dried to water content is 40%-50%, and period stirs 2 times;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 1:10, and the moistening time is 4h;

In described step 3), microwave power is 700W, and heat time heating time is 2-4min.

3. the preparation method of a kind of processed with honey Radix Codonopsis decoction pieces according to claim 1, is characterized in that,

In described step 1), bake out temperature is 70-80 DEG C, and being dried to water content is 50%-60%, and period stirs 1 time;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, and the moistening time is 4-10h;

In described step 3), microwave power is 700W, and heat time heating time is 2-4min.

4. the preparation method of a kind of processed with honey Radix Codonopsis decoction pieces according to claim 1, is characterized in that,

In described step 1), bake out temperature is 70-90 DEG C, and being dried to water content is 60%-70%, and period stirs 1 time;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 1:10, and the moistening time is 4-24h;

In described step 3), microwave power is 700W, and heat time heating time is 2-4min.

5. the preparation method of a kind of processed with honey Radix Codonopsis decoction pieces according to claim 1, it is characterized in that, in described step 1), bake out temperature is 70-90 DEG C, and being dried to water content is 40%-70%.

6. the preparation method of a kind of processed with honey Radix Codonopsis decoction pieces according to claim 1, is characterized in that,

In described step 1), being dried to water content is 50%;

Described step 2) in, the mass ratio of refined honey and Radix Codonopsis fresh slices is 2:10, and the moistening time is 8h;

In described step 3), microwave power is 700W, and heat time heating time is 2-4min.