CN105030843A - Lactobacillus reuteri drops and method for preparing same - Google Patents
Lactobacillus reuteri drops and method for preparing same Download PDFInfo
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- CN105030843A CN105030843A CN201510340289.6A CN201510340289A CN105030843A CN 105030843 A CN105030843 A CN 105030843A CN 201510340289 A CN201510340289 A CN 201510340289A CN 105030843 A CN105030843 A CN 105030843A
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Abstract
The invention discloses lactobacillus reuteri drops. The lactobacillus reuteri drops comprise, by weight, 25.0-30.0% of sunflower seed oil, 67.0-70.0% of medium-chain triglyceride, 1.7-2.1% of silicon dioxide and 1.0-1.5% of lactobacillus reuteri powder. A method for preparing the lactobacillus reuteri drops includes uniformly mixing the medium-chain triglyceride and the lactobacillus reuteri powder with each other to obtain first mixtures; adding the sunflower seed oil into the first mixtures and uniformly mixing the sunflower seed oil and the first mixtures with one another to obtain second mixtures; adding the silicon dioxide into the second mixtures and uniformly mixing the silicon dioxide and the second mixtures with one another to obtain the lactobacillus reuteri drops. The lactobacillus reuteri drops and the method have the advantages that probiotics in the lactobacillus reuteri drops are high in survival rate and long in survival time; when the refrigerating temperatures are 5 DEG C, the lactobacillus reuteri drops are optimal in stability, and the probiotics are high in survival rate and can survive for the longest time; the lactobacillus reuteri drops are quite convenient to take, and sufficient probiotics can be assuredly absorbed by a patient after only a few lactobacillus reuteri drops are dripped (into warm water or food).
Description
Technical field
The present invention relates to a kind of Lactobacillus reuteri drop and preparation method thereof, belong to probiotic bacteria drop technical field.
Background technology
Lactobacillus reuteri, latin name: Lactobacillusreuteri, character: the Campylobacter in slight irregular, circular distal.Lactobacillus reuteri belongs to turning property heterofermentation strain, sugar fermentation can produce CO2, lactic acid, acetic acid and ethanol.Lactobacillus reuteri often inhabits in the intestinal tract of humans and animals, harmless to humans and animals, has good biocompatibility.Its metabolism glycerol produces a kind of special antibacterial substance---Luo Yishi element (reuterin), and its main component is the monomer of 3-HPA (3-HPA), hydrate and Cyclodimerization body.Lactobacillus reuteri is the lactobacillus that almost can be present in all vertebratess and mammalian gut reported at present, is the beneficial bacteria of intestinal tract with beneficial function.
At present, the probiotic products on market has multiple dosage form, and as electuary, chewable tablet, packs mycopowder greatly, capsule etc., but the probiotic products of these dosage forms exists following defect: probiotic bacteria is easily oxidized, causes the mortality rate of probiotic bacteria too high; In addition, when production, mycopowder is easy to just dry at bottle mouth position, causes blocking, causes product can not drip out.
Summary of the invention
For above-mentioned prior art, the invention provides a kind of Lactobacillus reuteri drop, its probiotic bacteria survival rate is high, and the time-to-live is long, present invention also offers its preparation method
The present invention is achieved by the following technical solutions:
A kind of Lactobacillus reuteri drop, be made up of following raw material: Oleum Helianthi (not adding TBHQ or other antioxidants) 25.0 ~ 30.0%, medium chain triglyceride 67.0 ~ 70.0%, silicon dioxide (as mycopowder stabilizing agent) 1.7 ~ 2.1%, Lactobacillus reuteri mycopowder 1.0 ~ 1.5%, percentage; Most preferred, be made up of following raw material: Oleum Helianthi 27.6%, medium chain triglyceride 69.2%, silica 1 .9%, Lactobacillus reuteri mycopowder 1.3%.
Described Oleum Helianthi, medium chain triglyceride, silicon dioxide can obtain in conventional market purchase, and described Lactobacillus reuteri mycopowder can obtain in conventional market purchase, or prepares according to a conventional method.
The preparation method of described Lactobacillus reuteri drop is: first by medium chain triglyceride and Lactobacillus reuteri mycopowder mixing mixing, then add Oleum Helianthi, mixing mixing, then adds silicon dioxide, and mixing mixing, to obtain final product.
Further, described Oleum Helianthi, medium chain triglyceride, silicon dioxide all need sterilizing before combination, and sterilization method is: 121 DEG C of sterilizings 20 minutes, after sterilizing, are cooled to room temperature, for subsequent use.
Further, during described mixing, maintain the temperature at less than 20 DEG C.
Further, after obtained Lactobacillus reuteri drop, be filled into bottle, then in bottle, add a slice oxygen barrier sheet (solid plastic film), then aseptic packaging.The existence of oxygen barrier sheet, more effectively can allow probiotic bacteria condense upon the top layer of oxygen barrier sheet, extend the time-to-live of probiotic bacteria greatly.
Further, above-mentioned preparation process is carried out under " gnotobasis ", that is: manufacturing enterprise must be 100,000 grades of GMP aseptic environments, and the overall process of producing must have developer or technical staff's strict control on the scene, because whole production process is very responsive to the requirement of temperature, if the temperature of any one link is more than 50 DEG C, just likely cause the death in enormous quantities of Lactobacillus reuteri.
Lactobacillus reuteri drop of the present invention, the mycopowder stabilizing agent of a slice " oxygen barrier sheet " and specific proportions is added in each bottle of the inside, and under drop product being kept at the environment of temperature 5 ~ 15 DEG C simultaneously, under such circumstances, probiotic bacteria (Lactobacillus reuteri) can (temperature be lower, and the survival rate of probiotic bacteria is just higher continued survival 2 years or longer time; But can not lower than or close to 0 DEG C).During packaging, the internal diameter of the plug of bottleneck will be arranged rationally (guaranteeing that product can not cause the problem of blocking).
Lactobacillus reuteri drop of the present invention, its probiotic bacteria survival rate is high, and the time-to-live is long, and time at refrigerated storage temperature 5 DEG C, the optimal stability of product, the survival rate of probiotic bacteria is high, and the time-to-live of probiotic bacteria is the most permanent.Lactobacillus reuteri drop of the present invention, take very convenient (especially to child), relatively existing " probiotic electuary product " commercially, only needs to drip several (inside warm water or food), just can ensure that user absorbs enough probiotic bacterias.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Instrument, reagent, material etc. involved in following embodiment, unless otherwise noted, be existing conventional instrument, reagent, material etc. in prior art, obtain by regular commercial sources.Experimental technique involved in following embodiment, detection method etc., unless otherwise noted, are existing normal experiment method in prior art, detection method etc.
Embodiment 1 prepares Lactobacillus reuteri drop
Production equipment used is mainly as shown in table 1.
Table 1
Processing step is as follows:
(1) before batching first by ingredients pot and the pipeline be connected with filling machine, mycopowder pot and with the flexible pipe sterilizing of ingredients pot necklace, drying, cooling.
(2) medium chain triglyceride, Oleum Helianthi, Lactobacillus reuteri mycopowder (as shown in table 2, totally 7 group of formula) is weighed by prescription, for subsequent use.Attention: product cannot add the composition containing any antioxidant, otherwise the Lactobacillus reuteri very short time of the inside will oxidized (and being killed), so can only use the Oleum Helianthi not adding any antioxidant.
(3) medium chain triglyceride being dropped into band stirs in pressurized chuck pot ingredients pot; Oleum Helianthi being dropped into another band stirs in pressurized chuck pot ingredients pot; Seal respectively, jacket steam heats.
(4) respectively by two autoclave jacket steam heat temperature raisings, slightly open air valve to bleed off in pot valve-off after air, temperature is made to rise to 121 DEG C, keep after 20 minutes, close jacket steam, chuck passes into cooling water-cooled, limit cools, limit is stirred, and is cooled to room temperature (less than 20 DEG C, guarantee the same even ambient temperature of all solution in pot).
(5) mycopowder pot and the prior steam sterilization of connecting hose, and successively with the hot blast through aseptic filtration (HEAP defecator), cold air drying (guaranteeing in pot without some moisture), is chilled to room temperature (less than 20 DEG C).Under laminar flow hood in activity sterilizing room, the mycopowder weighed by side is added in mycopowder pot, seal and be connected with ingredients pot flexible pipe (sterilizing in advance, drying and cool) and medium chain ester sterilizing ingredients pot (connector in production scene with under fire environment be connected, in case living contaminants).
(6) with the compressed air through aseptic filtration, the mycopowder in mycopowder pot is all pressed in ingredients pot, simultaneously rapid stirring, adds after bacterium completes, reduce mixing speed, slowly stir, until mycopowder is all dispersed in medium chain ester.
(7) Oleum Helianthi of sterilizing cooling is slowly added in medium chain ester sterilizing ingredients pot, limit edged stirs, add rear Keep agitation, until the comprehensive dispersed mixing of medium chain ester, Oleum Helianthi and mycopowder, then in ingredients pot, continue to add silicon dioxide (silicon dioxide must sterilizing in advance: 121 DEG C of sterilizings 20 minutes, after sterilizing, are cooled to room temperature), mixing mixing, prepares fill.
(8) open the valve leading to filling machine, ingredients pot inner edge stirs, limit fill, mixing speed with do not make mycopowder gradually bottom degree for degree, too not fast yet, to guarantee that in every bottle product, mycopowder content is even.After fill, in bottle, add a slice oxygen barrier sheet (solid plastic film) (oxygen barrier sheet is of a size of: length 4 centimetres, width 0.7 centimetre, thickness 0.1 centimetre), then aseptic packaging.The existence of oxygen barrier sheet, more effectively can allow probiotic bacteria condense upon the top layer of oxygen barrier sheet, extend the time-to-live of probiotic bacteria greatly.
The present invention's Oleum Helianthi used is purchased from supermarket, ten thousand, China Resources, Fanyu, Guangzhou (manufacturer: Shanghai Standard Food Co., Ltd., trade name: many power Oleum Helianthi, article number: 841450159); Medium chain triglyceride is purchased from Bei Ge Bake, Guangzhou Food Co., Ltd (trade name: medium chain triglyceride, is also pungent certain herbaceous plants with big flowers acid glyceride, Malaysian import, manufacturer: PALM-OLEO (KLANG) SDN.BHD).
The Lactobacillus reuteri drop product prepared according to the method described above, the detection of its probiotic bacteria survival volume is followed the trail of as shown in table 2.
Table 2
Can be seen by table 2, by formula (Oleum Helianthi 27.6%, the medium chain triglyceride 69.2% of the best of the present invention, silica 1 .9%, Lactobacillus reuteri mycopowder 1.3%) product for preparing, the survival rate of its probiotic bacteria is the highest, is better than other formula.
Claims (9)
1. a Lactobacillus reuteri drop, it is characterized in that: be made up of following raw material: Oleum Helianthi 25.0 ~ 30.0%, medium chain triglyceride 67.0 ~ 70.0%, silica 1 .7 ~ 2.1%, Lactobacillus reuteri mycopowder 1.0 ~ 1.5%, percentage.
2. Lactobacillus reuteri drop according to claim 1, is characterized in that: be made up of following raw material: Oleum Helianthi 27.6%, medium chain triglyceride 69.2%, silica 1 .9%, Lactobacillus reuteri mycopowder 1.3%.
3. the preparation method of the Lactobacillus reuteri drop described in claim 1,2, is characterized in that: first by medium chain triglyceride and Lactobacillus reuteri mycopowder mixing mixing, then add Oleum Helianthi, mixing mixing, then add silicon dioxide, mixing mixing, to obtain final product.
4. the preparation method of Lactobacillus reuteri drop according to claim 3, it is characterized in that: described Oleum Helianthi, medium chain triglyceride, silicon dioxide all need sterilizing before combination, sterilization method is: 121 DEG C of sterilizings 20 minutes, after sterilizing, be cooled to room temperature, for subsequent use.
5. the preparation method of Lactobacillus reuteri drop according to claim 3, is characterized in that: during described mixing, maintains the temperature at less than 20 DEG C.
6. according to the preparation method of the Lactobacillus reuteri drop described in claim 3 ~ 5, it is characterized in that: after obtained Lactobacillus reuteri drop, be filled into bottle, then in bottle, add a slice oxygen barrier sheet, then aseptic packaging.
7. the preparation method of Lactobacillus reuteri drop according to claim 6, is characterized in that: described oxygen barrier sheet is solid plastic film.
8. the preparation method of Lactobacillus reuteri drop according to claim 6, is characterized in that: described oxygen barrier sheet is of a size of: length 4 centimetres, width 0.7 centimetre, thickness 0.1 centimetre.
9. according to the preparation method of the Lactobacillus reuteri drop described in claim 3 ~ 8, it is characterized in that: the overall process of described preparation is carried out under " gnotobasis ", that is: 100,000 grades of GMP aseptic environments.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105707897A (en) * | 2016-02-02 | 2016-06-29 | 上海交大昂立股份有限公司 | Probiotic lipid suspension drop and preparation method thereof |
| CN110447725A (en) * | 2019-09-12 | 2019-11-15 | 湖南天根乐微君科技有限公司 | The preparation method of probiotics tea oil drops and probiotics tea oil drops |
| CN111700123A (en) * | 2020-06-24 | 2020-09-25 | 广州彤博士健康科技有限公司 | High-oleic acid sunflower seed oil formula containing medium chain triglyceride and preparation method thereof |
| CN112494529A (en) * | 2020-12-17 | 2021-03-16 | 拉格比(广东)健康科技有限公司 | Probiotic drop for improving intestinal colic and flatulence and preparation method thereof |
| CN115531424A (en) * | 2021-06-30 | 2022-12-30 | 锦乔生物科技有限公司 | Probiotic drops |
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| CN102481323A (en) * | 2009-07-31 | 2012-05-30 | 雀巢产品技术援助有限公司 | Nutritional Composition For Breast-fed Infants Or Pets With Probiotics And Selected Nutrients |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707897A (en) * | 2016-02-02 | 2016-06-29 | 上海交大昂立股份有限公司 | Probiotic lipid suspension drop and preparation method thereof |
| CN110447725A (en) * | 2019-09-12 | 2019-11-15 | 湖南天根乐微君科技有限公司 | The preparation method of probiotics tea oil drops and probiotics tea oil drops |
| CN111700123A (en) * | 2020-06-24 | 2020-09-25 | 广州彤博士健康科技有限公司 | High-oleic acid sunflower seed oil formula containing medium chain triglyceride and preparation method thereof |
| CN112494529A (en) * | 2020-12-17 | 2021-03-16 | 拉格比(广东)健康科技有限公司 | Probiotic drop for improving intestinal colic and flatulence and preparation method thereof |
| CN115531424A (en) * | 2021-06-30 | 2022-12-30 | 锦乔生物科技有限公司 | Probiotic drops |
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