CN105021443A - Turntable type frozen section staining device - Google Patents
Turntable type frozen section staining device Download PDFInfo
- Publication number
- CN105021443A CN105021443A CN201510466887.8A CN201510466887A CN105021443A CN 105021443 A CN105021443 A CN 105021443A CN 201510466887 A CN201510466887 A CN 201510466887A CN 105021443 A CN105021443 A CN 105021443A
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- lifting
- cylinder
- rotating
- rotating disk
- disc type
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- 238000010186 staining Methods 0.000 title claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims abstract description 37
- 238000004043 dyeing Methods 0.000 claims description 33
- 230000008014 freezing Effects 0.000 claims description 24
- 238000007710 freezing Methods 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 9
- 238000003825 pressing Methods 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000007599 discharging Methods 0.000 abstract 1
- 238000011010 flushing procedure Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 description 11
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a turntable type frozen section staining device and belongs to the technical field of staining devices. The turntable type frozen section staining device is technically characterized by comprising a machine body, wherein at least one set of staining unit is arranged on the upper end face of the machine body; each staining unit mainly comprises a turntable module, a lifting clamping mechanism arranged on the side edge of the turntable module and a flushing tank arranged on the side edge of the lifting clamping mechanism; each turntable module mainly comprises a turntable, and a sample injection cylinder, a sample discharging cylinder and a plurality of staining cylinders which are uniformly distributed on the turntable along the circumferential direction; each lifting clamping mechanism mainly comprises a lifting support arranged on the machine body, a first lifting sliding block arranged on the lifting support, a lifting driving mechanism connected with the first lifting sliding block, a rotating mechanism arranged on the first sliding block, a cylinder arranged on the rotating mechanism and a clamp which is connected with the cylinder and is used for clamping a slide. The invention aims at providing the turntable type frozen section staining device which is compact in structure, low in manufacturing cost and high in working efficiency and is used for staining a frozen section.
Description
Technical field
The present invention relates to a kind of dyeing instrument, more particularly, particularly relate to a kind of rotating disc type freezing microtome section dyeing instrument.
Background technology
Traditional freezing microtome section dyeing is manual operation process, Color depends on experience and the custom of operating personnel, and the staining reagent used relates to volatile toxic chemical substance, operating personnel's Long Term Contact health can produce bad reaction, damage healthy, moreover, also increase cost of labor and time.Although I has developed the product of efficiency that can ensure freezing microtome section dyeing quality and improve freezing microtome section dyeing, quality stability, cost-saving in have much room for improvement.Therefore, need to make further improvement to rotating disc type freezing microtome section dyeing instrument.
Summary of the invention
The object of the invention is to for above-mentioned the deficiencies in the prior art, provide a kind of compact conformation, manufacturing cost lower and the rotating disc type freezing microtome section dyeing instrument that work efficiency is higher.
Technical scheme of the present invention is achieved in that a kind of rotating disc type freezing microtome section dyeing instrument, comprise body, wherein said body upper surface is provided with at least one group of dye unit, described dye unit primarily of rotating disk module, be arranged on the lifting clamping device of rotating disk module side, be arranged on the bath groove composition of lifting clamping device side; Described rotating disk module, cap-opening mechanism are connected with main control unit respectively with lifting clamping device; Described rotating disk module primarily of rotating disk, be circumferentially distributed on sample introduction cylinder on rotating disk, go out sample cylinder and some staining jars composition; Described lifting clamping device primarily of the lifting support be arranged on body, the first lifting slider be arranged on lifting support, the lift drive mechanism be connected with the first lifting slider, the rotating mechanism be arranged on the first slide block, be arranged on the cylinder on rotating mechanism and be connected with cylinder and fixture for clamping slide forms; Cylinder pipeline connects external compressed air source.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described rotating disk primarily of circle support, be arranged on carriage center and the rotating shaft be fixedly connected with support and the rotary tray motor that is located at rotating shaft side form; Sample introduction cylinder, go out sample cylinder and some staining jars are circumferentially arranged on support respectively; The power output shaft of rotating shaft lower end and rotary tray motor is equipped with relative belt pulley, and two belt pulleys are linked by driving-belt, and described rotary tray motor is connected with main control unit.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described sample introduction cylinder, the peristome going out sample cylinder and some staining jars are equipped with cylinder cap, and described cylinder cap is hinged on each cylinder body one end near disk edge, is provided with the operator's console extended to outside cylinder body at the hinged end of cylinder cap; The body of rotating disk side is provided with the cap-opening mechanism matched with operator's console.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described cap-opening mechanism primarily of the bearing of uncapping be arranged on body, be vertically movably arranged on first screw mandrel of uncapping on bearing by bearing, be arranged on the motor of uncapping of bearing side of uncapping, be vertically arranged on the guidepost of uncapping on bearing, be movably set on guidepost and with the second lifting slider that the first wire rod thread connects, with the connecting link that the second lifting slider is connected and be located at pressing plate on connecting link and corresponding with operator's console and form; Connecting link is vertically arranged; Described connecting link one end is connected with the second lifting slider, and the other end is connected with fixed head, and described pressing plate is arranged on bottom fixed head; Described uncap motor and the first screw mandrel are coordinated by belt pulley and belt and link, described in motor of uncapping be connected with main control unit.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described lift drive mechanism is made up of the second screw mandrel be vertically movably arranged on by bearing on lifting support, the lifting motor being arranged on lifting support side and the lifting guidepost be vertically arranged on lifting support; Described first lifting slider is set in and is elevated on guidepost and is connected with the second wire rod thread; Described lifting motor is connected with main control unit.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described rotating mechanism primarily of being located at the electric rotating machine of lifting support side, the swingle be flexibly connected with the first lifting slider by bearing, the cantilever that is arranged on swingle upper end formed; Electric rotating machine power output shaft and swingle are equipped with corresponding belt pulley, and two belt pulleys are linked by driving-belt; Described cylinder is arranged on bottom cantilever free end; Described cylinder is connected with main control unit respectively with the solenoid valve on external compressed air source pipeline and electric rotating machine.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described cylinder is gas pawl cylinder; Described fixture is made up of the clamping plate that two pieces are matched, and two clamping plates is connected with gas pawl cylinder respectively.
In above-mentioned rotating disc type freezing microtome section dyeing instrument, described fixture is provided with liquid level sensor, and liquid level sensor is connected with main control unit.
After the present invention adopts said structure, complete, both improve work efficiency, and in turn ensure that dyeing quality by existing manual operation dyeing is become mechanical automation, product stability is good and can be cost-saving better.Particularly, by arranging the rotating disk module, lifting clamping device and the bath groove that cooperatively interact, make product with compact structure, lifting clamping device can move less scope and namely realize dyeing and rinsing, and work efficiency is high; Meanwhile, by arranging cap-opening mechanism, making each cylinder body on rotating disk can realize existing use and now opening, the used time does not close at once, effectively prevents maloperation and can ensure safety in production.
Accompanying drawing explanation
Below in conjunction with the embodiment in accompanying drawing, the present invention is described in further detail, but do not form any limitation of the invention.
Fig. 1 is structural representation of the present invention;
Fig. 2 is the structural representation of the single dye unit of the present invention;
Fig. 3 is the structural representation of rotating disk module of the present invention and cap-opening mechanism;
Fig. 4 is the inner structure schematic diagram of rotating disk of the present invention;
Fig. 5 is the perspective view that the present invention is elevated clamping device;
Fig. 6 is the sectional structure schematic diagram that the present invention is elevated clamping device; ,
Fig. 7 is the perspective view of cap-opening mechanism of the present invention;
Fig. 8 is the sectional structure schematic diagram of cap-opening mechanism of the present invention.
In figure: body 1, rotating disk module 2, rotating disk 2a, sample introduction cylinder 2b, go out sample cylinder 2c, staining jar 2d, support 2e, rotating shaft 2f, rotary tray motor 2g, lifting clamping device 3, lifting support 3a, first lifting slider 3b, cylinder 3c, fixture 3d, bath groove 4, main control unit 5, lift drive mechanism 6, second screw mandrel 6a, lifting motor 6b, lifting guidepost 6c, rotating mechanism 7, electric rotating machine 7a, swingle 7b, cantilever 7c, cylinder cap 8, operator's console 8a, cap-opening mechanism 9, uncap bearing 9a, first screw mandrel 9b, uncap motor 9c, guidepost 9d, second lifting slider 9e, connecting link 9f, pressing plate 9g, fixed head 9h, liquid level sensor 10.
Embodiment
Consult shown in Fig. 1 to Fig. 3, a kind of rotating disc type freezing microtome section dyeing instrument of the present invention, comprises body 1, be provided with at least one group of dye unit in body 1 upper surface, be provided with two groups of dye unit in the present embodiment, certainly, according to actual conditions, also more groups of dye unit can be set.
Dye unit forms primarily of rotating disk module 2, the lifting clamping device 3 being arranged on rotating disk module 2 side, the bath groove 4 that is arranged on lifting clamping device 3 side; Described rotating disk module 2 is connected with main control unit 5 respectively with lifting clamping device 3.
Consult shown in Fig. 5 and Fig. 6, lifting clamping device 3 primarily of the lifting support 3a be arranged on body 1, the first lifting slider 3b be arranged on lifting support 3a, the lift drive mechanism 6 be connected with the first lifting slider 3b, the rotating mechanism 7 be arranged on the first lifting slider 3b, be arranged on the cylinder 3c on rotating mechanism 7 and be connected with cylinder 3c and fixture 3d for clamping slide forms; Cylinder 3c pipeline connects external compressed air source.Particularly, in the present embodiment, described lift drive mechanism 6 is made up of the second screw mandrel 6a be vertically movably arranged on by bearing on lifting support 3a, the lifting motor 6b being arranged on lifting support 3a side and the lifting guidepost 6c be vertically arranged on lifting support 3a; Described first lifting slider 3b is set in and is elevated on guidepost 6c and is threaded with the second screw mandrel 6a; Described lifting motor 6b is connected with main control unit 5.Control lifting motor 6b by main control unit 5, drive the second screw mandrel 6a to rotate, and then drive the first lifting slider 3b to complete lifting action.When dye unit is multiple, main control unit 5 can adopt control eventually to combine the mode performing and control.Namely each dye unit connects corresponding execution control module respectively, and each control module that performs is connected with terminal control unit again, and terminal control unit is as master control.General be exactly for control each dye unit start and stop.
Meanwhile, described rotating mechanism 7 primarily of being located at the electric rotating machine 7a of lifting support 3a side, the swingle 7b be flexibly connected with the first lifting slider 3b by bearing, the cantilever 7c that is arranged on swingle 7b upper end forms; Electric rotating machine 7a power output shaft and swingle 7b are equipped with corresponding belt pulley, and two belt pulleys are linked by driving-belt, thus make swingle 7b complete spinning movement.Described cylinder 3c is arranged on bottom cantilever 7c free end; Described cylinder 3c is connected with main control unit 5 respectively with the solenoid valve on external compressed air source pipeline and electric rotating machine 7a.In the present embodiment, cylinder 3c is preferably gas pawl cylinder; Described fixture 3d is made up of the clamping plate that two pieces are matched, and two clamping plates is connected with gas pawl cylinder respectively.Preferably, fixture is provided with liquid level sensor 10, liquid level sensor 10 is connected with main control unit 5.The liquid level in each cylinder is detected, with the downward storke of control cylinder 3c by liquid level sensor 10.
Rotating disk module 2 primarily of rotating disk 2a, be circumferentially distributed on sample introduction cylinder 2b on rotating disk 2a, go out sample cylinder 2c and some staining jar 2d form; Consult shown in Fig. 4, in the present embodiment, described rotating disk 2a primarily of circle support 2e, be arranged on support 2e center and the rotating shaft 2f be fixedly connected with support and the rotary tray motor 2g that is located at rotating shaft 2f side forms; Sample introduction cylinder 2b, go out sample cylinder 2c and some staining jar 2d are circumferentially arranged on support 2e respectively; The power output shaft of rotating shaft 2f lower end and rotary tray motor 2g is equipped with relative belt pulley, and two belt pulleys are linked by driving-belt, and described rotary tray motor 2g is connected with main control unit 5.Meanwhile, be equipped with cylinder cap 8 at sample introduction cylinder 2b, the peristome that goes out sample cylinder 2c and some staining jar 2d, described cylinder cap 8 is hinged on each cylinder body one end near rotating disk 2a edge, is provided with the operator's console 8a extended to outside cylinder body at the hinged end of cylinder cap 8; The body 1 of rotating disk 2a side is provided with the cap-opening mechanism 9 matched with operator's console 8a.Consult shown in Fig. 7 and Fig. 8, described cap-opening mechanism 9 primarily of the bearing 9a that uncaps be arranged on body 1, be vertically movably arranged on by bearing the first screw mandrel 9b uncapped on bearing 9a, be arranged on the motor 9c that uncaps of bearing 9a side of uncapping, be vertically arranged on the guidepost 9d uncapped on bearing 9a, be movably set on guidepost 9d and the second lifting slider 9e, the connecting link 9f be connected with the second lifting slider 9e that are threaded with the first screw mandrel 9b and be located at pressing plate 9g on connecting link 9f and corresponding with operator's console 8a and form; Connecting link 9f is vertically arranged; Described connecting link 9f one end is connected with the second lifting slider 9e, and the other end is connected with fixed head 9h, and described pressing plate 9g is arranged on bottom fixed head 9h; Described uncap motor 9c and the first screw mandrel 9b are coordinated by belt pulley and belt and link, described in the motor 9c that uncaps be connected with main control unit 5.Controlling by main control unit 5 the motor 9c that uncaps drives the first screw mandrel 9b to rotate, thus drive the second lifting slider 9e is elevated, and makes pressing plate 9g coordinate with operator's console 8a action of uncapping.
During use, by equipment power-on, under the control of main control unit 5, complete following step:
The first step: loading;
Second step: press " start " key and start;
3rd step: rotating disk 2a forwards working position to sample;
4th: lifting clamping device 3 declines, fixture 3d clamps slide and rises, and lifting clamping device 3 turns to above bath groove 4;
5th step: rotating disk forwards working position to next reagent tank;
6th step: cap-opening mechanism 9 declines, opens the cylinder cap 8 of corresponding cylinder body;
7th step: lifting clamping device 3 turns to working position, lifting clamping device 3 declines, and slide immerses in dyeing liquor and dyes, and continues the regular hour;
8th step: after dyeing time arrives, lifting clamping device 3 rises, and clips slide and leaves dyeing liquor, and lifting clamping device 3 turns to above bath groove 4, and cap-opening mechanism 9 rises, and builds the lid of reagent tank;
Above 5th step is the dyeing course of a staining jar to the 8th step, one has 12 to 16 staining jars, and the process of each staining jar is identical, if some cylinders have dyeed need the step of bath, then being elevated clamping device 3 forwards to above bath groove 4, lifting clamping device 3 declines, and slide immerses in flowing water and carries out rinsing, after certain hour, lifting clamping device 3 clips slide and rises, turn to working position, carry out the dyeing of next cylinder, repeat the process of the 5th step to the 8th step.
Above illustrated embodiment is better embodiment of the present invention, only be used for conveniently the present invention being described, not any pro forma restriction is done to the present invention, have in any art and usually know the knowledgeable, if do not depart from the present invention carry in the scope of technical characteristic, utilize the Equivalent embodiments that the done local of disclosed technology contents is changed or modified, and do not depart from technical characteristic content of the present invention, all still belong in the scope of the technology of the present invention feature.
Claims (8)
1. a rotating disc type freezing microtome section dyeing instrument, comprise body (1), it is characterized in that, described body (1) upper surface is provided with at least one group of dye unit, described dye unit primarily of rotating disk module (2), be arranged on rotating disk module (2) side lifting clamping device (3), be arranged on lifting clamping device (3) side bath groove (4) composition; Described rotating disk module (2) is connected with main control unit (5) respectively with lifting clamping device (3); Described rotating disk module (2) primarily of rotating disk (2a), be circumferentially distributed on sample introduction cylinder (2b) on rotating disk (2a), go out sample cylinder (2c) and some staining jars (2d) composition; Described lifting clamping device (3) primarily of the lifting support (3a) be arranged on body (1), the first lifting slider (3b) be arranged on lifting support (3a), the lift drive mechanism (6) be connected with the first lifting slider (3b), the rotating mechanism (7) be arranged on the first lifting slider (3b), be arranged on the cylinder (3c) on rotating mechanism (7) and be connected with cylinder (3c) and fixture (3d) for clamping slide forms; Cylinder (3c) pipeline connects external compressed air source.
2. rotating disc type freezing microtome section dyeing instrument according to claim 1, it is characterized in that, described rotating disk (2a) primarily of circle support (2e), be arranged on support (2e) center and the rotating shaft (2f) be fixedly connected with support and the rotary tray motor (2g) that is located at rotating shaft (2f) side form; Sample introduction cylinder (2b), go out sample cylinder (2c) and some staining jars (2d) are circumferentially arranged on support (2e) respectively; The power output shaft of rotating shaft (2f) lower end and rotary tray motor (2g) is equipped with relative belt pulley, and two belt pulleys are linked by driving-belt, and described rotary tray motor (2g) is connected with main control unit (5).
3. rotating disc type freezing microtome section dyeing instrument according to claim 1, it is characterized in that, described sample introduction cylinder (2b), the peristome going out sample cylinder (2c) and some staining jars (2d) are equipped with cylinder cap (8), described cylinder cap (8) is hinged on each cylinder body one end near rotating disk (2a) edge, is provided with the operator's console (8a) extended to outside cylinder body at the hinged end of cylinder cap (8); The body (1) of rotating disk (2a) side is provided with the cap-opening mechanism (9) matched with operator's console (8a).
4. rotating disc type freezing microtome section dyeing instrument according to claim 3, it is characterized in that, described cap-opening mechanism (9) is primarily of the bearing of uncapping (9a) be arranged on body (1), the first screw mandrel (9b) on bearing of uncapping (9a) is vertically movably arranged on by bearing, be arranged on the motor of uncapping (9c) of bearing of uncapping (9a) side, vertically be arranged on the guidepost (9d) on bearing of uncapping (9a), be movably set in guidepost (9d) to go up and the second lifting slider (9e) be threaded with the first screw mandrel (9b), with the connecting link (9f) that the second lifting slider (9e) connects and be located at the upper and pressing plate (9g) corresponding with operator's console (8a) of connecting link (9f) and form, connecting link (9f) is vertically arranged, described connecting link (9f) one end is connected with the second lifting slider (9e), and the other end is connected with fixed head (9h), and described pressing plate (9g) is arranged on fixed head (9h) bottom, described motor of uncapping (9c) and the first screw mandrel (9b) are coordinated by belt pulley and belt and link, described in motor (9c) of uncapping be connected with main control unit (5).
5. rotating disc type freezing microtome section dyeing instrument according to claim 1, it is characterized in that, described lift drive mechanism (6) is made up of the second screw mandrel (6a) be vertically movably arranged on by bearing on lifting support (3a), the lifting motor (6b) being arranged on lifting support (3a) side and the lifting guidepost (6c) be vertically arranged on lifting support (3a); Described first lifting slider (3b) is set in and is elevated on guidepost (6c) and is threaded with the second screw mandrel (6a); Described lifting motor (6b) is connected with main control unit (5).
6. rotating disc type freezing microtome section dyeing instrument according to claim 1, it is characterized in that, described rotating mechanism (7) primarily of being located at the electric rotating machine (7a) of lifting support (3a) side, the swingle (7b) be flexibly connected with the first lifting slider (3b) by bearing, the cantilever (7c) that is arranged on swingle (7b) upper end formed; Electric rotating machine (7a) power output shaft and swingle (7b) are equipped with corresponding belt pulley, and two belt pulleys are linked by driving-belt; Described cylinder (3c) is arranged on bottom cantilever (7c) free end; Described cylinder (3c) is connected with main control unit (5) respectively with the solenoid valve on external compressed air source pipeline and electric rotating machine (7a).
7. the rotating disc type freezing microtome section dyeing instrument according to claim 1 or 6, is characterized in that, described cylinder (3c) is gas pawl cylinder; Described fixture (3d) is made up of the clamping plate that two pieces are matched, and two clamping plates is connected with gas pawl cylinder respectively.
8. a kind of rotating disc type freezing microtome section dyeing instrument according to claim 1 or 6, it is characterized in that, described fixture is provided with liquid level sensor (10), and liquid level sensor (10) is connected with main control unit (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510466887.8A CN105021443B (en) | 2015-08-03 | 2015-08-03 | Rotating disc type freezing microtome section dyeing instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510466887.8A CN105021443B (en) | 2015-08-03 | 2015-08-03 | Rotating disc type freezing microtome section dyeing instrument |
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| Publication Number | Publication Date |
|---|---|
| CN105021443A true CN105021443A (en) | 2015-11-04 |
| CN105021443B CN105021443B (en) | 2017-08-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510466887.8A Active CN105021443B (en) | 2015-08-03 | 2015-08-03 | Rotating disc type freezing microtome section dyeing instrument |
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| Country | Link |
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| CN (1) | CN105021443B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108398305A (en) * | 2018-04-28 | 2018-08-14 | 福州迈新生物技术开发有限公司 | A kind of paraffin section de-waxing device |
| CN110595857A (en) * | 2019-09-11 | 2019-12-20 | 宁波江丰生物信息技术有限公司 | Freezing slice dyeing machine and slice dyeing method thereof |
| CN111896729A (en) * | 2020-08-09 | 2020-11-06 | 深圳大学总医院 | Immunohistochemistry staining device |
| WO2020258155A1 (en) * | 2019-06-27 | 2020-12-30 | 深圳迈瑞生物医疗电子股份有限公司 | Slide maker and control method therefor |
| CN113340697A (en) * | 2021-05-31 | 2021-09-03 | 佳木斯大学 | Auxiliary device for manufacturing tumor tissue pathological section and using method thereof |
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| CN203502277U (en) * | 2013-08-22 | 2014-03-26 | 武汉兰丁医学高科技有限公司 | Low-pollution cellular tissue dyeing machine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111896729A (en) * | 2020-08-09 | 2020-11-06 | 深圳大学总医院 | Immunohistochemistry staining device |
| CN113340697A (en) * | 2021-05-31 | 2021-09-03 | 佳木斯大学 | Auxiliary device for manufacturing tumor tissue pathological section and using method thereof |
| CN113340697B (en) * | 2021-05-31 | 2023-10-20 | 佳木斯大学 | Auxiliary device for preparing pathological section of tumor tissue and use method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105021443B (en) | 2017-08-29 |
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