CN105017236A - C-aryl glucoside derivative, as well as medical composition, preparation method and application thereof - Google Patents

C-aryl glucoside derivative, as well as medical composition, preparation method and application thereof Download PDF

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CN105017236A
CN105017236A CN201510151483.XA CN201510151483A CN105017236A CN 105017236 A CN105017236 A CN 105017236A CN 201510151483 A CN201510151483 A CN 201510151483A CN 105017236 A CN105017236 A CN 105017236A
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compound
aryl
reaction
heterocyclylalkyl
solvent
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高大新
杨和平
王培�
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Shanghai de Novo Pharmatech Co Ltd
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Shanghai de Novo Pharmatech Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
    • C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
    • C07D407/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Abstract

The invention relates to a C-aryl glucoside derivative, as well as a medical composition, a preparation method and application thereof. The preparation method comprises the following steps of: method I, in a solvent, under the action of alkali, performing a deacetylation protecting group reaction on compounds 1-f; method II: 1, performing a Mitsunobu reaction on components 2-g and (as shown in the description); and 2, performing a deacetylation protecting group reaction on the compounds 2-f obtained in the step 1; and method III: 1, mixing compounds 3-g and (as shown in the description), and performing a nucleophilic substitution reaction; and 2, performing a deacetylation protecting group reaction on the compounds 3-f obtained in the step 1. The medical composition comprises the C-aryl glucoside derivative, salt and/or prodrug of the C-aryl glucoside derivative which are/is acceptable in pharmacy, and auxiliary materials. The invention further relates to the C- aryl glucoside derivative, and application of salt or medical compositions of the C- aryl glucoside derivative, which is acceptable in pharmacy, for preparing SGLT inhibitors. The C- aryl glucoside derivative disclosed by the invention provides a new direction for research of the SGLT inhibitors. (As shown in the description)

Description

C-aryl glycoside derivative, its pharmaceutical composition, preparation method and application
Technical field
The present invention relates to a kind of C-aryl glycoside derivative, its steric isomer, prodrug or pharmacy acceptable salt, its pharmaceutical composition, preparation method and application.
Background technology
The metabolism class disease of diabetes to be classes with hyperglycemia be feature, current global incidence is in ascendant trend year by year, wherein, the patient of 90% is had for type ii diabetes in diabetic subject, because of the hyperglycemia that excessive hepatic glucose produces and periphery insulin resistance causes, (pharmacy is in progress, 2003,27:88-91).At present, the most frequently used medicine being applicable to type ii diabetes has sulfonylurea, biguanides, alpha-carbon glycosides enzyme inhibitors and insulin type etc. to have control blood sugar increasing and hypoglycemic effect preferably, but along with many untoward reactions relevant to medicine, as body weight increase, insulin secretion minimizing, hypoglycemia, gastrointestinal side effect etc.Therefore need to develop safer, effective, can be oral anti-diabetic class medicine.
The glucose transporter (sodium-glucose co-transporters, SGLTs) that sodium relies on plays keying action in maintenance blood sugar for human body is stable.SGLT-1 is mainly distributed in small intestine, kidney, heart and brain, and its main Physiological Function is complete absorption to glucose at small intestine.SGLT-2 is specific is distributed in renal proximal tubules S1 position, be responsible for the heavily absorption of about 90% glucose, residue 10% is completed by the SGLT-1 being positioned at proximal convoluted tubule S3 position, therefore, the heavily absorption of specific inhibition kidney to glucose can be carried out by suppressing the activity of SGLT-2, discharge unnecessary glucose by urine and reach hypoglycemic effect of falling, not having body weight to increase and hypoglycemic risk simultaneously.Therefore, SGLT particularly SGLT2 inhibitor be very promising candidate's antidiabetic medicine (Handlon, A.L., Expert Opin.Ther.Patents (2005) 15 (11): 1531-1540; Am JPhysiol Renal Physiol, 2001,280:10-18).
At present, existing a lot of SGLT2 inhibitor is disclosed, such as, and Handlon, A.L., Expert Opin.Ther.Patents (2005) 15 (11): 1531-1540; Nature Reviews Drug Discovery, 2010, Vol.9, No.7,551-559; WO 01/27128, WO 02/083066, WO 03/099836, US2003/0114390, WO 04/063209, WO 2005/012326, US 2005/0209166.Wherein, there is the clean (Canagliflozin of the Kan Gelie of Janssen Pharmaceutica under Johnson & Johnson by the SGLT-2 inhibitor of FDA's approval listing, on March 29th, 2013 is granted), the clean (Dapagliflozin of Da Gelie of AstraZeneca and Bristol-Myers Squibb Co., on January 8th, 2014 is granted), the Li Gelie clean (Empagliflozin) of Boehringer Ingelheim and Li Lai company only obtains European people from drug administration at present and ratifies (on March 21st, 2014 is granted) with the listing of the pharmaceutical prod council (CHMP).
Summary of the invention
Technical problem to be solved by this invention is, provides a kind of C-aryl glycoside derivative, its steric isomer, prodrug or the pharmacy acceptable salt inhibited for the glucose transporter (SGLT) of sodium dependence, its pharmaceutical composition, preparation method and application.
The present invention relates to a kind of such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I;
Wherein, X is O or S; L is CH 2, CD 2, C=O or CF 2; Z is CH 2, S, O or singly-bound; W is singly-bound or (CH 2) n, n=1,2 or 3;
Y is bicyclic radicals; The preferred C of described bicyclic radicals 5-8cycloalkyl C 6aryl, C 5-8cycloalkyl C 3-5heteroaryl, C 3-7heterocyclylalkyl C 6aryl, C 3-7heterocyclylalkyl C 3-5heteroaryl, C 6aryl C 6aryl, C 3-5heteroaryl C 6aryl or C 3-5heteroaryl C 3-5heteroaryl; Described bicyclic radicals can be substituted in optional position by following one or more group: H, alkyl, CN, halogen, CF 3, OH, amino, C 2-6alkynyl, C 2-6thiazolinyl, alkoxyl group, halogenated alkoxy, cycloalkyl, cycloalkyl oxy, cycloalkylalkyl, cycloalkyl alkoxy, Heterocyclylalkyl, Heterocyclylalkyl oxygen base, hetercycloalkylalkyl, heterocycloalkylalkoxy, alkylamino, carbonyl, COOH, COOR 7, COR 7, CONR 7r 7a,-NHCOR 7,-NHSO 2r 7, aryl, heteroaryl, alkyl sulphonyl, aryl sulfonyl, or heteroarylsulfonyl, wherein, in the substituting group of described bicyclic radicals, described substituting group can further replace by following one or more group: alkyl, halogen, CF 3, OH, CN, amino, alkoxyl group or halogenated alkoxy;
R 1for H, halogen, CN, alkyl, alkoxyl group, halogenated alkoxy, OCD 3, OC 2d 5or CF 3;
R 2for H, alkyl, halogen, CF 3, CN, OH, amino, alkoxyl group, halogenated alkoxy, OCD 3, OC 2d 5, C 2-6alkynyl, C 2-6thiazolinyl, cycloalkyl, Heterocyclylalkyl, alkylamino, carbonyl, COOH, COOR 7, COR 7, CONR 7r 7a,-NHCOR 7,-NHSO 2r 7, aryl, heteroaryl, alkyl sulphonyl, aryl sulfonyl or heteroarylsulfonyl;
R 7and R 7abe selected from alkyl, cycloalkyl or Heterocyclylalkyl independently of one another, or, R 7and R 7athe Heterocyclylalkyl of 3-7 unit is formed together with the atom N that they connect.
As described R 2during for halogen, the preferred chlorine of described halogen.
At R 7and R 7aformed together with the atom N that they connect in the Heterocyclylalkyl of 3-7 unit, the Heterocyclylalkyl of described 3-7 unit also can contain 1-3 following atom or group further: N, O, S, SO and SO 2.
At R 7and R 7aformed together with the atom N that they connect in the Heterocyclylalkyl of 3-7 unit, the Heterocyclylalkyl of described 3-7 unit can be replaced by alkyl and/or methylsulfonyl.
In the present invention, in Y, described C 3-7heterocyclylalkyl C 6the preferred C of aryl 4 ~ 6heterocyclylalkyl C 6aryl.Described C 3-5heteroaryl C 6the preferred C of aryl 3-4heteroaryl C 6aryl.
In the present invention, Y more preferably in (but being not limited to) following Y1 to Y24 any one:
Wherein, R 3, R 4, R 5, R 6, R 6a, R 6band R 6cbe H, alkyl, CN, halogen, CF independently of one another 3, OH, amino, C 2-6alkynyl, C 2-6thiazolinyl, alkoxyl group, halogenated alkoxy, cycloalkyl, cycloalkyl oxy, cycloalkylalkyl, cycloalkyl alkoxy, Heterocyclylalkyl, Heterocyclylalkyl oxygen base, hetercycloalkylalkyl, heterocycloalkylalkoxy, alkylamino, carbonyl, COOH, COOR 7, COR 7, CONR 7r 7a,-NHCOR 7,-NHSO 2r 7, aryl, heteroaryl, alkyl sulphonyl, aryl sulfonyl or heteroarylsulfonyl;
Described R 3, R 4, R 5, R 6, R 6a, R 6band R 6ccan be replaced by following any group further: alkyl, halogen, CF 3, OH, CN, amino, alkoxyl group or halogenated alkoxy;
Described R 6aand R 6bthe cycloalkyl of 3-8 unit is formed together with the atom that can also connect with them, or 3-8 unit Heterocyclylalkyl.Described 3-8 unit Heterocyclylalkyl is preferably containing 1-3 following atom or group: N, O, S, SO and SO 2.The cycloalkyl of described 3-8 unit or 3-8 unit Heterocyclylalkyl can by alkyl and/or halogen substiuted.
In the present invention, described Y is preferably
more preferably * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
Described is preferably IA, IB or IC such as formula the C-aryl glycoside derivative shown in I,
In IA, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; R 1, R 2, R 3, R 4, R 5, R 6definition ditto described in;
In IB, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; R 1, R 2, R 3, R 4, R 5, R 6a, R 6b, R 6cdefinition ditto described in; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type;
In IC, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; R 1, R 2, R 3, R 4, R 5, R 6a, R 6b, R 6cdefinition ditto described in; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
Above-mentioned IB and IC general formula comprises the S type absolute configuration of carbon of * mark, R type absolute configuration and racemic modification.
Preferred such as formula the C-aryl glycoside derivative shown in IA:
Wherein, X is O; L is CH 2; Z is O; W is (CH 2) n, n=1 or 2; R 1for H or F; R 2for H, F, Cl, CN, CH 3or OCH 3; R 3, R 4, R 5be separately H, methyl or F; R 6for H.
Preferred such as formula the C-aryl glycoside derivative shown in IB:
Wherein, X is O; L is CH 2; Z is O; W is (CH 2) n, n=1 or 2; R 1for H or F; R 2for H, F, Cl, CN, CH 3or OCH 3; R 3, R 4, R 5be separately H, methyl or F; R 6a, R 6band R 6cindependently be H or CH 3; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
Preferred such as formula the C-aryl glycoside derivative shown in IC:
In IC, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; R 1for H or F; R 2for H, F, Cl, CN, CH 3or OCH 3; R 3, R 4, R 5be separately H, methyl or F; R 6a, R 6band R 6cindependently be H or CH 3; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
Described is the arbitrary compound of following I-1 ~ I-148 such as formula the C-aryl glycoside derivative shown in I:
Present invention also offers the described preparation method such as formula the C-aryl glycoside derivative shown in I, it is following either method:
Method one: described preparation method comprises the steps: in solvent, under the effect of alkali, carries out the reaction of deacetylation protecting group by compound 1-f;
Method two: described preparation method comprises the steps: 1), in solvent, under the effect of condensing agent, by compound 2-g with carry out Mitsunobu reaction; 2), in solvent, under the effect of alkali, by step 1) the compound 2-f that obtains carries out the reaction of deacetylation protecting group;
Method three: described preparation method comprises the steps: 1, in solvent, under the effect of alkali, by compound 3-g with mixing, carries out nucleophilic substitution reaction; 2, in solvent, under the effect of alkali, compound 3-f step 1 obtained carries out the reaction of deacetylation protecting group;
Wherein, V is chlorine, bromine or iodine; Described in all the other each groups and letter definition are all the same.
In method one, the condition of described deacetylation protecting group reaction and step can be condition and the step of the deacetylation protecting group reaction of this area routine, and the present invention is following reaction conditions particularly preferably: described solvent particular methanol or the mixed solvent of methyl alcohol, tetrahydrofuran (THF) and water; When adopting mixed solvent, the volume ratio of methyl alcohol, tetrahydrofuran (THF) and water preferably 4: 1: 0.5 ~ 0.5: 1: 0.5, consumption preferably 5 ~ 15mL/mmol compound 1-f of described solvent; Described alkali preferred as alkali alcoholate or alkali metal hydroxide; Described alkali is sodium methylate or lithium hydroxide more preferably; The mol ratio of described alkali and compound 1-f preferably 0.1: 1 ~ 2: 1; The temperature of described reaction preferably 10 ~ 30 DEG C; Described reaction detects by TLC, as the terminal of reaction when generally disappearing using compound 1-f, and preferably 0.5 ~ 12 hour; After described reaction terminates, be also further purified product by aftertreatment, preferably include following steps: after reaction system acetic acid is adjusted pH to 6 ~ 8, removal of solvent under reduced pressure, solid residue thin layer prepares plate or purification by silica gel column chromatography.Described thin layer is prepared the step of plate purifying or purification by silica gel column chromatography and condition and be can be step and the condition that thin layer conventional in this area prepares plate purifying or purification by silica gel column chromatography.In the present invention, described deacetylation protecting group reaction preferably adopts sodium methylate and methanol system, or lithium hydroxide, methyl alcohol, tetrahydrofuran (THF) and aqueous systems.In described lithium hydroxide, methyl alcohol, tetrahydrofuran (THF) and aqueous systems, the volume ratio of methyl alcohol, tetrahydrofuran (THF) and water preferably 4: 1: 0.5 ~ 0.5: 1: 0.5.
In method one, the preparation method of described compound 1-f can be the ordinary method of this type of reaction in this area, preferably includes following steps: in solvent, compound 1-e is carried out ethanoyl protective reaction, then carry out recrystallization;
Described ethanoyl protective reaction can be the ethanoyl protective reaction of this area routine, its reaction conditions and step all can reference: J.Med.Chem.2008,51,1145-1149, particularly preferably following condition in the present invention: the preferred methylene dichloride of described solvent; Consumption preferably 5 ~ 20mL/mmol compound 1-e of described solvent; In described ethanoyl protective reaction, the preferred diacetyl oxide of ethanoyl protection reagent; The mol ratio of described ethanoyl protection reagent and compound 1-e preferably 5: 1 ~ 20: 1.In the step of described recrystallization and condition, the solvent preferred volume concentration of employing is more than or equal to 50% and is less than the aqueous ethanolic solution of 100%, or dehydrated alcohol.Consumption preferably 3 ~ 10mL/mmol compound 1-e of the ethanol of described recrystallization.The temperature of described recrystallization preferably 50 ~ 100 DEG C.
In method one, the preparation method of described compound 1-e can be the ordinary method of this type of reaction in this area, preferably includes following steps: in solvent, is mixed by compound 1-d with triethyl silicane and boron trifluoride diethyl etherate system, carry out reduction reaction;
Described solvent can be the conventional solvent of this type of reaction of this area, the mixed solvent of preferred methylene dichloride and acetonitrile, wherein, and described methylene dichloride and the volume ratio of acetonitrile preferably 1: 1 ~ 2: 1.The consumption of described solvent does not generally affect the carrying out of reaction, preferably 5 ~ 15mL/mmol compound 1-d.In described triethyl silicane and boron trifluoride diethyl etherate system, the mol ratio of triethyl silicane and boron trifluoride diethyl etherate preferably 1: 1 ~ 1.5: 1.The mol ratio of described triethyl silicane and compound 1-d preferably 1.5: 1 ~ 3: 1.The temperature of described reduction reaction preferably-20 DEG C ~ 10 DEG C.The process of described reaction detects by HPLC or TLC, as the terminal of reaction when generally disappearing using compound 1-d, and preferably 2 ~ 5 hours.After described reaction terminates, be also further purified product by aftertreatment, preferably include following steps: after saturated sodium bicarbonate aqueous solution cancellation reaction, reaction system organic solvent extraction, organic phase is dry, and decompression removing organic solvent obtains compound 1-e.The compound 1-e that above-mentioned aftertreatment obtains is usually not purified is directly used in next step reaction.
In method one, the preparation method of described compound 1-d can be the ordinary method of this type of reaction in this area, can reference: J.Med.Chem.2008, 51, 1145-1149, the present invention preferably includes following steps: in solvent, at-78 DEG C ~-60 DEG C, compound 1-c and alkali metal lithium reagent mix are reacted 0.5 ~ 1 hour, again with 2, 3, 4, (preparation method can reference: J.Med.Chem.2008 for 6-tetra--O-(TMS)-D-glucopyra saccharon, 51, 1145-1149) hybrid reaction 0.5 ~ 2 hour (preferably 1 hour), at 10 ~ 30 DEG C, mix with the methanol solution of methylsulfonic acid again, react,
Wherein, Q is bromine or iodine.
Described solvent can be the conventional solvent of this type of reaction of this area, the mixed solvent of preferred tetrahydrofuran (THF) and toluene.In described tetrahydrofuran (THF) and the mixed solvent of toluene, the volume ratio of tetrahydrofuran (THF) and toluene preferably 1: 1 ~ 1: 4.Described organolithium reagent preferred tertiary butyllithium or n-Butyl Lithium; Work as R 2during for CN, described organolithium reagent preferred tertiary butyllithium.The mol ratio of described organolithium reagent and compound 1-c preferably 0.9: 1 ~ 2: 1.2,3,4,6-tetra-described-O-(TMS)-D-glucopyra saccharons and the mol ratio of compound 1-c preferably 1: 1 ~ 1.5: 1.The volumetric molar concentration preferably 0.5 ~ 1.2mol/L of the methanol solution of described methylsulfonic acid.The mol ratio of described methylsulfonic acid and compound 1-c preferably 1.2: 1 ~ 3.0: 1.The process of described reaction detects by HPLC or TLC, as the terminal of reaction when generally disappearing using compound 1-c.
In method one, the preparation method of described compound 1-c preferably includes following steps: in solvent, is mixed by compound 1-b with triethyl silicane and boron trifluoride diethyl etherate system, carries out reduction reaction;
Wherein, Q is bromine or iodine.
Described solvent can be the conventional solvent of this type of reaction of this area, the preferably mixed solvent of 1,2-ethylene dichloride and acetonitrile, wherein, and the volume ratio of 1,2-ethylene dichloride and acetonitrile preferably 1: 2 ~ 2: 1.The consumption of described solvent does not generally affect the carrying out of reaction, preferably 5 ~ 15mL/mmol compound 1-b.In described triethyl silicane and boron trifluoride diethyl etherate system, the mol ratio of triethyl silicane and boron trifluoride diethyl etherate preferably 1.2: 1 ~ 3: 1.The mol ratio of described triethyl silicane and compound 1-b preferably 1.5: 1 ~ 4: 1.The temperature of described reaction preferably 0 ~ 30 DEG C.The process of described reduction reaction detects by HPLC or TLC, general when compound 1-b disappears as the terminal reacted.After described reduction reaction terminates, also be further purified product by post-processing step, described post-processing step preferably includes: after saturated sodium bicarbonate aqueous solution cancellation reaction, reaction system organic solvent extraction, organic phase is dry, and decompression removing organic solvent obtains compound 1-e.Described compound 1-e is by silica gel column chromatography or recrystallization purifying.
In method one, prepare in the method for compound 1-c, work as R 2during for CN, the method preparing compound 1-c preferably includes following steps: in solvent, is first mixed with sodium borohydride and methanol system by compound 1-b, after carrying out reduction reaction, mix with triethyl silicane and boron trifluoride diethyl etherate system again and react, obtain compound 1-c.Described sodium borohydride and methanol system can be the sodium borohydride methanol system of this area routine, wherein, and the mol ratio of sodium borohydride and compound 1-b preferably 1: 1 ~ 3: 1; The consumption of described solvent does not generally affect the carrying out of reaction, preferably 5 ~ 20mL/mmol compound 1-b.
In method one, the preparation method of described compound 1-b preferably includes following steps: in solvent, in the presence of a base, by compound 1-a with mixing, carries out nucleophilic substitution reaction;
Wherein, Q is bromine or iodine.
Described solvent can be this area, and this type of reacts Conventional solvents used, preferred DMF.The amount of described solvent is generally the carrying out not affecting reaction, preferably 2 ~ 20mL/mmol compound 1-a.Described alkali can be alkali-metal alcoholate, preferred potassium tert.-butoxide.The molar weight of described alkali is preferably 0.9 ~ 2.5 times of compound 1-a.Described with the mol ratio of compound 1-a preferably 1.0: 1 ~ 2.0: 1.The temperature of described reaction preferably 20 ~ 50 DEG C.The process of described reaction detects by HPLC or TLC, as the terminal of reaction when generally disappearing using compound 1-a, and preferably 1 ~ 18 hour.After described reaction terminates, product is further purified by post-processing step, preferably include following post-processing step: after organic solvent diluting reaction system, by water and saturated common salt water washing organic phase, dry, removal of solvent under reduced pressure, obtains compound 1-b, then through silica gel column chromatography or recrystallization purifying.Described column chromatography or the method for recrystallization and condition can be selected by the method for the column chromatography of this area routine or recrystallization and condition.The solvent preferred volume concentration that described recrystallization adopts is more than or equal to 50% and is less than the aqueous ethanolic solution of 100%, or dehydrated alcohol.The consumption preferably 2 ~ 50mL/mmol of described solvent.
In method two, step 1) in, described compound 2-g prepares by the method for this area routine, such as reference: WO2011/048148A2.Described solvent can be this type of reaction common solvent of this area, preferred tetrahydrofuran (THF).The consumption of described solvent does not generally affect the carrying out of reaction, preferred 5-20ml/mmol compound 2-g.Described condensing agent can be the conventional condensation reagent system of this type of reaction of this area, triphenylphosphine and diethyl azodiformate (DEAD), or triphenylphosphine and diisopropyl azodiformate (DIAD).Described triphenylphosphine and the mol ratio of diethyl azodiformate preferably 1: 1 ~ 1.5: 1.Described triphenylphosphine and the mol ratio of diisopropyl azodiformate preferably 1: 1 ~ 1.5: 1.The mol ratio of described condensing agent and compound 2-g preferably 1: 1 ~ 3: 1.Described with the mol ratio of compound 2-g preferably 1: 1 ~ 3: 1.The temperature of reaction preferably 0 DEG C ~ 30 DEG C of described Mitsunobu reaction.The process of described reaction detects by TLC or HPLC, as the terminal of reaction when generally disappearing using compound 2-g, and preferred 2-15 hour.After described reaction terminates, product can also be further purified by post-processing step, described post-processing step preferably includes following steps: after organic solvent diluting reaction system, by aqueous ammonium chloride solution and saturated common salt water washing organic phase, dry, removal of solvent under reduced pressure, obtains compound 2-g, then through purification by silica gel column chromatography.The step of described purification by silica gel column chromatography and condition all can be selected by the step of the purification by silica gel column chromatography of this area routine and condition.
In method two; step 2) in; the condition of described deacetylation protecting group reaction and step can be condition and the step of the deacetylation protecting group reaction of this area routine, and optimum condition of the present invention is identical with step with the condition of the deacetylation protecting group reaction described in aforesaid method one.
In method three, in step 1, described compound 3-g prepares by the method for this area routine, such as reference: WO2011/048148A2.Described solvent can be the conventional solvent of this type of reaction of this area, preferred DMF solution or dimethyl sulfoxide (DMSO).The consumption of described solvent does not generally affect the carrying out of reaction, preferably 5 ~ 20mL/mmol compound 3-g.The preferred cesium carbonate of described alkali or salt of wormwood.The mol ratio of described alkali and compound 3-g preferably 1 ~ 2.5.Described with the mol ratio of compound 3-g preferably 1 ~ 2.5.The temperature of described nucleophilic substitution reaction preferably 40 DEG C-65 DEG C.The process of described nucleophilic substitution reaction detects by HPLC or TLC, as the terminal of reaction when generally disappearing using compound 3-g, and preferred 2-15 hour.After described reaction terminates, product can also be further purified by aftertreatment, described aftertreatment preferably includes following steps: after diluted ethyl acetate reaction system, by aqueous ammonium chloride solution and saturated common salt water washing organic phase, dry, removal of solvent under reduced pressure, obtains compound 3-g, then through purification by silica gel column chromatography.The step of described purification by silica gel column chromatography and condition all can be selected by the step of the purification by silica gel column chromatography of this area routine and condition.
In method three; in step 2; the condition of described deacetylation protecting group reaction and step can be condition and the step of the deacetylation protecting group reaction of this area routine, and optimum condition of the present invention is identical with step with the condition of the deacetylation protecting group reaction described in aforesaid method one.
In the present invention, involved compound all prepares by aforesaid method one.
The invention further relates to the compound of following arbitrary structure:
wherein, each group definition as previously mentioned; Compound f can be compound 1-f, compound 2-f or compound 3-f.
Except as otherwise noted, the following term occurred in the present invention has following implication:
Term " alkyl " refers to the saturated straight chain or branched hydrocarbyl that comprise 1-20 carbon atom, preferably 1 ~ 10 carbon atom, more preferably 1 ~ 8 carbon atom, such as: methyl, ethyl, n-propyl, sec.-propyl, normal-butyl, the tertiary butyl, isobutyl-, amyl group, hexyl, heptyl, octyl group, nonyl, decyl, 4,4-dimethyl amyl group, 2,2,4-tri-methyl-amyl, undecyl, dodecyl, and their various isomer etc.
Term " cycloalkyl " refers to and comprises the saturated of 3-20 carbon atom or monocycle, dicyclo or the tricyclic hydrocarbon base of part unsaturated (comprising 1 or 2 double bond), preferred 3-10 unit monocycle alkyl, more preferably 5-8 unit monocycle alkyl, such as: cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, suberyl, ring octyl group, ring decyl, cyclo-dodecyl, cyclohexenyl, bornyl, decahydro naphthyl, dicyclo [2.2.1] heptenyl etc.Term " C5-8 cycloalkyl " refers to the cycloalkyl comprising 5-8 carbon atom.
Term " Heterocyclylalkyl " refers to by carbon atom and the non-aromatic cyclic radical being selected from the saturated or part unsaturated (comprising 1 or 2 double bond) that the heteroatomss such as nitrogen, oxygen or sulphur form, this cyclic group can be monocycle, dicyclo or three cyclic groups, preferred 3-10 unit Heterocyclylalkyl, more preferably 5-8 unit Heterocyclylalkyl, most preferably 5-8 unit monocyclic heterocycloalkyl.In the present invention, heteroatoms number preferably 1 or 2 in Heterocyclylalkyl, the nitrogen in Heterocyclylalkyl, carbon or sulphur atom are optionally oxidized.Nitrogen-atoms can optionally be replaced by other groups further and form tertiary amine or quaternary ammonium salt.Such as: '-aziridino, tetrahydrofuran (THF)-2-base, morpholine-4-base, thiomorpholine-4-base, thiomorpholine-S-oxide-4-base, piperidin-1-yl, N-Alkylpiperidine-4-base, pyrrolidin-1-yl, N-alkyl pyrrolidine-2-base, piperazine-1-base, 4-alkylpiperazine-1-base, with deng.
Term " C 3-7heterocyclylalkyl " refer to the monocyclic heterocycloalkyl comprising 3-7 carbon atom, wherein heteroatoms number is 1-2.Term " C 4-6heterocyclylalkyl " refer to the monocyclic heterocycloalkyl comprising 4-6 carbon atom, wherein heteroatoms number is 1-2.
Term " cycloalkylalkyl " is referred to and is connected by alkyl between cycloalkyl with mother nucleus structure.Thus, " cycloalkylalkyl " comprises the definition of abovementioned alkyl and cycloalkyl.
Term " hetercycloalkylalkyl " is referred to and is connected by alkyl between Heterocyclylalkyl with mother nucleus structure.Thus, " hetercycloalkylalkyl " comprises the definition of abovementioned alkyl and Heterocyclylalkyl.
What term " alkoxyl group " referred to be connected by oxo bridge has described carbonatoms object ring-type or acyclic alkyl groups, comprises alkyl oxy, cycloalkyl oxy and Heterocyclylalkyl oxygen base.Thus, " alkoxyl group " comprises the definition of abovementioned alkyl, Heterocyclylalkyl and cycloalkyl.
Term " cycloalkyl alkoxy " refers to the group of the hydrogen evolution in cycloalkyl substituted alkoxyl group on alkyl.Thus, " cycloalkyl alkoxy " comprises the definition of above-mentioned cycloalkyl and alkoxyl group.
Term " heterocycloalkylalkoxy " refers to the group of the hydrogen evolution in Heterocyclylalkyl substituted alkoxy on alkyl.Thus, " heterocycloalkylalkoxy " comprises the definition of above-mentioned Heterocyclylalkyl and alkoxyl group.
Term " thiazolinyl " refers to straight chain, side chain or the non-aromatic alkyl of ring-type containing at least 1 carbon-carbon double bond.Wherein can there is 1-3 carbon-carbon double bond, preferably there is 1 carbon-carbon double bond.Term " C 2-6thiazolinyl " refer to the thiazolinyl with 2-6 carbon atom, comprise vinyl, propenyl, butenyl, 2-methyl butene base and cyclohexenyl.Described thiazolinyl can be substituted.
Term " alkynyl " refers to straight chain, side chain or cyclic hydrocarbon group containing at least 1 carbon carbon triple bond.Wherein can there is 1-3 carbon carbon triple bond, preferably there is 1 carbon carbon triple bond.Term " C 2-6alkynyl " refer to the alkynyl with 2-6 carbon atom, comprise ethynyl, proyl, butynyl and 3-methylbutynyl
Term " aryl " refers to any stable 6-10 unit's monocycle or bicyclic aromatic groups, such as: phenyl, naphthyl, tetralyl, 2,3-indanyls or xenyl etc.Term " C 6aryl " refer to 6 yuan of monocyclic aryl, such as phenyl.
The heteroatoms that the carbon atom that term " heteroaryl " refers at least 1 ring is selected from nitrogen, oxygen or sulphur replaces the aromatic group formed, and it can be 5-7 unit's single ring architecture or 7-12 unit twin nuclei, preferred 5-6 unit heteroaryl.In the present invention, heteroatoms number preferably 1 or 2, comprise indazolyl, iso indazolyl, indyl, pseudoindoyl, benzofuryl, benzothienyl, benzo [d] [1,3] dioxolanyl, benzothiazolyl, benzoxazolyl, quinolyl, isoquinolyl, quinazolyl etc.Term " C 3-5heteroaryl " refer to the bicyclic heteroaryl comprising 3-5 carbon atom, wherein heteroatoms number is 1-2.Term " C 3-4heteroaryl " refer to the bicyclic heteroaryl comprising 3-4 carbon atom, wherein heteroatoms number is 1-2.
Term " bicyclic radicals " refer to two ring texturees share each other condensed ring structure that two adjacent atoms are formed or and ring structure, ring texture can comprise aryl, heteroaryl, cycloalkyl and Heterocyclylalkyl.A ring is had at least to be aromatic nucleus in indication twin nuclei of the present invention.In the present invention, C 5-8cycloalkyl C 6the specific embodiment of aryl includes but not limited to: benzocyclobutene, 2,3-dihydro-1-H-indenes, 1,2,3,4-naphthane, 6,7,8,9-tetrahydrochysene-5H-benzo [7] annulene, 6,9-dihydro-5H-benzo [7] annulenes or 5,6,7,8,9,10-hexahydrobenzene is [8] annulene also; C 5-8cycloalkyl C 3-5the specific embodiment of heteroaryl includes but not limited to: 2,3-cyclopentenopyridine, 5,6-dihydro-4H-cyclopentyl [B] thiophene or 5,6-dihydro-4H-cyclopentyl [B] furans; C 4-6heterocyclylalkyl C 6the specific embodiment of aryl includes but not limited to: 2,3-Dihydrobenzofuranes 1,3-dihydroisobenzofuran indoline, 2,3-dihydrobenzos [b] thiophene, dihydrobenzo piperazine are muttered, 1,2,3,4-tetrahydroquinoline, 2,3-dihydros-Isosorbide-5-Nitrae-benzdioxan, 3,4-dihydro-2H-1,4-benzoxazine, or c 3-7heterocyclylalkyl C 3-5the specific embodiment of heteroaryl includes but not limited to: naphthyridines, or c 6aryl C 6the specific embodiment of aryl includes but not limited to: naphthalene; C 3-4heteroaryl C 6the specific embodiment of aryl includes but not limited to: cumarone, thionaphthene, benzopyrrole, benzothiazole, benzoxazoles, indazole, benzo pyridazine, benzoglyoxaline, indoles, quinoline, isoquinoline 99.9; C 3-5heteroaryl C 3-5the specific embodiment of heteroaryl includes but not limited to: purine or pyridine of talking endlessly;
When there being a ring to be non-aromatic ring in bicyclic radicals, be interconnected preferably by any carbon atom on non-aromatic ring and the side base W shown in formula I structure, the preferred cycloalkyl of non-aromatic ring or Heterocyclylalkyl.When in bicyclic radicals, two rings are aromatic nucleus, the side base W preferably by structure shown in any carbon atom on two rings and formula I is interconnected, and aromatic nucleus can be aryl or heteroaryl." bicyclic radicals " described in the present invention comprises the above-mentioned definition for " aryl ", " Heterocyclylalkyl ", " cycloalkyl " and " heteroaryl ".
Term " halogen " represents fluorine, chlorine, bromine or iodine.
Term " haloalkyl " refers to the alkyl replaced arbitrarily by halogen.Thus, " haloalkyl " comprises the definition of above halogen and alkyl.
Term " halogenated alkoxy " refers to the alkoxyl group replaced arbitrarily by halogen.Thus, " halogenated alkoxy " comprises the definition of above halogen and alkoxyl group.
Term " amino " refers to-NH 2, term " alkylamino " refer at least one hydrogen atom amino upper replace by alkyl.Thus, " alkylamino " comprises abovementioned alkyl and amino definition.
Term " alkylsulfonyl " refers to-SO 2-.
Term " alkyl sulphonyl " refers between alkyl and mother nucleus structure by alkylsulfonyl-SO 2-be connected.Therefore, " alkyl sulphonyl " comprises the definition of abovementioned alkyl and alkylsulfonyl.
Term " aryl sulfonyl " refers between aryl and mother nucleus structure by alkylsulfonyl-SO 2-be connected.Therefore, " aryl sulfonyl " comprises the definition of above-mentioned aryl and alkylsulfonyl.
Term " heteroarylsulfonyl " refers between heteroaryl and mother nucleus structure by alkylsulfonyl-SO 2-be connected.Thus, " heteroarylsulfonyl " comprises the definition of above alkylsulfonyl and heteroaryl.
The invention still further relates to a kind of pharmaceutical composition, it comprises described such as formula C-aryl glycoside derivative, isotropic substance substitutive derivative, its pharmacy acceptable salt and/or the prodrug shown in I, and pharmaceutically acceptable auxiliary material.
Described steric isomer comprises enantiomer and diastereomer.
Described isotropic substance substitutive derivative comprises: in formula I arbitrary hydrogen atom by 1-5 D atom replace obtain isotropic substance substitutive derivative, arbitrary carbon atom to be replaced in the isotropic substance substitutive derivative or formula I obtained Sauerstoffatom arbitrarily and is replaced the isotropic substance substitutive derivative obtained by 1-3 oxygen 18 atom by 1-3 carbon 14 atom in formula I.
Described pharmaceutical composition can contain the medicine for the treatment of diabetes or the therapeutical agent of other kind of other kind further.
Described prodrug to refer to through various approach after organism administration, can discharge the prodrug of active compound of the present invention.Described prodrug includes but not limited to the ester and hydrate etc. of the aryl glycoside of C-shown in formula I derivative.
Described auxiliary material can be pharmaceutically acceptable carrier, thinner and/or vehicle etc.
The invention still further relates to described such as formula the application of C-aryl glycoside derivative, its pharmacy acceptable salt or the described pharmaceutical composition shown in I in preparation SGLT inhibitor.
The invention still further relates to the described application for the treatment of in preparation such as formula C-aryl glycoside derivative, its pharmacy acceptable salt or the described pharmaceutical composition shown in I in the medicine of the relative disease of SGLT mediation.
The relative disease of described SGLT mediation generally comprises: diabetes (comprising II type and I type), diabetic complication, obesity, high blood pressure disease, and any metabolic trouble relevant to diabetes.
In above-mentioned application, described such as formula C-aryl glycoside derivative, its pharmacy acceptable salt or the described pharmaceutical composition shown in I, also can further with the second therapeutical agent coupling.
One or more in the described preferred Remedies for diabetes of the second therapeutical agent, treating diabetic complications agent, lipid-lowering therapy agent, obesity treatment agent and hypertension therapeutic agent.
In the present invention, described method one preferably synthetic route is:
In the present invention, room temperature refers to 10 ~ 30 DEG C.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can arbitrary combination, obtains the preferred embodiments of the invention.
Agents useful for same of the present invention and raw material are all commercially.
Positive progressive effect of the present invention is: aryl glycoside derivative of the present invention has extraordinary restraining effect for SGLT-2, and effectively can treat the relative disease of SGLT mediation, be novel antidiabetic medicine.
Embodiment
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, conventionally and condition, or selects according to catalogue.
The structure of all compounds of the present invention by nucleus magnetic resonance ( 1h NMR) and/or mass spectrometric detection (MS) qualification.
1h nmr chemical displacement (δ) is with PPM record (10 -6).NMR is undertaken by BrukerAVANCE-400 spectrograph.Suitable solvent is deuterochloroform (CDCl 3), deuterated methanol (CD 3oD), deuterated dimethyl sulfoxide (DMSO-d 6), tetramethylsilane is as interior mark (TMS).
Algorithm (MS) is measured by Agilent 1200HPLC/6120 mass spectrograph, uses XBridge C18,4.6 × 50mm, 3.5 μm, condition of gradient elution one: 80-5% solvent orange 2 A 1with 20-95% solvent B 1(1.8 minutes), then 95% solvent B 1with 5% solvent orange 2 A 1(more than 3 minutes), percentage ratio is the percent by volume that a certain solvent accounts for total solvent volume.Solvent orange 2 A 1: the aqueous solution of 0.01% trifluoroacetic acid (TFA); Solvent B 1: the acetonitrile solution of 0.01% trifluoroacetic acid; Percentage ratio is the percent by volume that solute accounts for solution.Condition of gradient elution two: 80-5% solvent orange 2 A 2with 20-95% solvent B 2(1.5 minutes), then 95% solvent B 2with 5% solvent orange 2 A 2(more than 2 minutes), percentage ratio is the percent by volume that a certain solvent accounts for total solvent volume.Solvent orange 2 A 2: the aqueous solution of the bicarbonate of ammonia of 10mM; Solvent B 2: acetonitrile.
The chiral analysis of the compounds of this invention uses supercritical fluid chromatography analyser SFC MethodStation (Thar, Waters), and analysis condition is A, B 1, B 2or C; Use chiral high performance liquid chromatography analyser Chiral HPLC (Agilent 1200), analysis condition is D.Determined wavelength is 214nm or 254nm.
Chiral analysis condition A: with chiral column OJ-H 4.6*250mm, 5um (DAICEL), moving phase is carbonic acid gas: methyl alcohol (containing 0.1% diethylamide, percentage ratio is percent by volume)=60: 40, flow velocity is 3mL/min, and column temperature is 40 DEG C;
Chiral analysis condition B 1: with chiral column OZ-H 4.6*250mm, 5um (DAICEL), moving phase is carbonic acid gas: methyl alcohol (containing 0.1% diethylamide, percentage ratio is percent by volume)=55: 45, and flow velocity is 3mL/min, and column temperature is 40 DEG C;
Chiral analysis condition B 2: with chiral column OZ-H 4.6*150mm, 5um (DAICEL), moving phase is carbonic acid gas: methyl alcohol (containing 0.2% methanol ammonia, percentage ratio is percent by volume)=50: 50, and flow velocity is 3mL/min, and column temperature is 40 DEG C;
Chiral analysis condition C: with chiral column OJ-H 4.6*250mm, 5um (DAICEL), moving phase is carbonic acid gas: methyl alcohol (containing 0.1% diethylamide, percentage ratio is percent by volume)=55: 45, flow velocity is 3mL/min, and column temperature is 40 DEG C.
Chiral analysis condition D: with chiral column IC 4.6*250mm, 5um (DAICEL), moving phase is that normal hexane is (containing 0.1% diethylamide, percentage ratio is percent by volume): methyl alcohol is (containing 0.1% diethylamide, percentage ratio is percent by volume)=80: 20, flow velocity is 1mL/min, and column temperature is 40 DEG C.
The chirality preparation of the compounds of this invention uses chiral high performance liquid chromatography (Chiral HPLC) and supercritical fluid chromatography (SFC), total following A, B, C, D and E five kinds of splitting condition:
Chiral high performance liquid chromatography preparing instrument uses HPLC-Gilso GX-281Method Station, splitting condition A: chiral column CHIRALPAK OZ-H 30*250mm 5um (Daicel), moving phase is that normal hexane is (containing 0.1% diethylamide, percentage ratio is percent by volume): ethanol is (containing 0.1% diethylamide, percentage ratio is percent by volume)=30: 70, flow velocity is 30g/min, column temperature is 25 DEG C, sample concentration: 12.5mg/mL, sample size: 4mL; Splitting condition B: chiral column OZ-H 30*250mm 5um (Daicel), moving phase is methyl alcohol (containing 0.5% diethylamide, percentage ratio is percent by volume), flow velocity is 50mL/min, column temperature is 35 DEG C, sample concentration: 18mg/mL, sample size: 1mL; Splitting condition C: chiral column CHIRALPAK IC 20*250mm 5um (DAICEL), moving phase is normal hexane: ethanol=80: 20, and flow velocity is 50mL/min, and column temperature is 40 DEG C, sample concentration: 6 ~ 12mg/mL, sample size: 2.0mL;
Supercritical fluid chromatography analyser uses SFC-80 (Thar, Waters), splitting condition D: chiral column OJ-H 20*250mm, 5um (DAICEL), moving phase is carbonic acid gas: methyl alcohol is (containing 0.5% ammoniacal liquor, percentage ratio is percent by volume)=45: 55, flow velocity is 80g/min, and column temperature is 35 DEG C; Splitting condition E: chiral column CHIRALPAK OZ-H 20*250mm 5um (DAICEL), moving phase is carbonic acid gas: methyl alcohol is (containing 0.2% methanol ammonia, percentage ratio is percent by volume)=50: 50, flow velocity is flow velocity is 80g/min, and column temperature is 35 DEG C.
The optically-active of the compounds of this invention uses RUDOLPH Automatic Polarimeter automatic polarimeter to measure, and light source is D light, and sample pool length is 1cm.
Thin-layer silicon offset plate is Yantai Huanghai Sea HSGF254 or Qingdao GF254 silica-gel plate.Column chromatography generally uses Yantai Huanghai Sea 200-300 order silica gel as carrier.
Embodiment 1:2,3,4,6-tetra-synthesis of-O-(TMS)-D-glucopyra saccharon
By maltonic acid delta-lactone (100.0g, 0.56mol) be dissolved in tetrahydrofuran (THF) (700mL), triethylamine (511g is added under ice bath, trimethylchlorosilane (427g is dripped 5.05mol), 3.93mol) about 1 hour, dropwise rear ice bath and stir 2 hours, system rises to stirred overnight at room temperature.Add ethyl acetate (2L), wash with saturated biphosphate sodium water solution (1L × 2), water (1L) and saturated aqueous common salt (1L × 2) respectively, organic phase anhydrous magnesium sulfate drying, filtration, solvent under reduced pressure is revolved steaming obtain colorless oil, add toluene (100mL × 2), underpressure distillation obtains 2 to constant weight, 3,4,6-tetra--O-(TMS)-D-glucopyra saccharon (254g, productive rate 97%) is colourless liquid.
1HNMR(400MHz，CDCl 3)：δ4.17-4.20(m，1H)，4.01(d，J＝8.0Hz，1H)，3.92(t，J＝7.2Hz，1H)，3.75-3.85(m，3H)，0.21(S，9H)，0.19(S，9H)，0.18(S，9H)，0.14(S，9H)。
Method 1
The synthesis of the bromo-2-cyanobenzoic acid of embodiment 2:5-
In tetrahydrofuran (THF) (30mL), be cooled to-30 DEG C by molten for diisopropylamine (6.67g, 65.9mmol) under nitrogen protection, slowly drip the hexane solution (2.5M, 26.3mL, 65.9mmol) of n-Butyl Lithium.Reaction system is cooled to-70 DEG C after stirring 30 minutes at-30 DEG C, slowly drips bromobenzylcyanide (10g, 54.9mmol), keeps-70 DEG C to react after 30 minutes and adds carbon dioxide solid.Reaction system slowly rises to stirred overnight at room temperature.Add water (50mL) and ethyl acetate (100mL), be separated organic phase, aqueous phase ethyl acetate (100mL × 2) extracts, merge organic phase, use saturated common salt water washing, remove desolventizing obtain the bromo-2-cyanobenzoic acid of 5-(7.25g, productive rate: 58%) be white solid with anhydrous magnesium sulfate drying, filtration, vacuum rotary steam.
m/z：[M-H] -225.9
The synthesis of the bromo-O-Anisic Acid of embodiment 3:5-
2-methoxyl group-5-bromobenzaldehyde (20g, 93.0mmol) is dissolved into (100mL) in acetone, after being cooled to 5 DEG C, in 2 hours, adds potassium permanganate solid (14.7g, 93.0mmol) in batches.Finish, reaction system room temperature for overnight.Filtered by mixture, filtrate adjusts pH=1 with 5M hydrochloric acid, separates out faint yellow solid, filters.The bromo-O-Anisic Acid of 5-(10.5g, yield: 49%) is obtained after vacuum-drying.
m/z：[M-H] -228.9
The bromo-2-p-methoxy-phenyl of embodiment 4:(5-) synthesis of (4-fluorophenyl) ketone
The bromo-2-hydroxy phenyl of step 1:(5-) (4-fluorophenyl) ketone
The 2-methoxyl group-5-bromo-benzoic acid (1.5g, 6.49mmol) embodiment 3 obtained is mixed in methylene dichloride (20mL), drips oxalyl chloride (1.24g, 9.74mmol).Reaction system at room temperature stirs after 2 hours and is removed by excessive oxalyl chloride vacuum rotary steam, and resistates is dissolved in fluorobenzene (6.24g, 64.9mmol), adds aluminum chloride (0.95g, 7.14mmol) at 10 DEG C.Reaction solution reacted after 2 hours at 75 DEG C to be poured in frozen water (30g), aqueous phase ethyl acetate (30mL × 3) extracts, merge organic phase, anhydrous sodium sulfate drying, vacuum rotary steam obtains (the bromo-2-hydroxy phenyl of 5-) (4-fluorophenyl) ketone (1.57g, productive rate: 82%).
The bromo-2-p-methoxy-phenyl of step 2:(5-) (4-fluorophenyl) ketone
(the bromo-2-hydroxy phenyl of 5-) (4-fluorophenyl) ketone (920mg that step 1 is prepared, 3.12mmol) be dissolved in acetone (10mL), add salt of wormwood (861mg, 6.24mmol), methyl iodide (663mg, 4.68mmol).Reaction system stirs 1 hour at 10 DEG C, and mixture filters, and filtrate decompression is revolved and steamed (the bromo-2-p-methoxy-phenyl of 5-) (4-fluorophenyl) ketone (930mg, yield: 97%).
1H NMR(400MHz，CDCl 3)：δ7.82-7.86(m，2H)，7.58(dd，J＝8.8，2.8Hz，1H)，7.46(d，J＝2.8Hz，1H)，7.13(t，J＝8.8Hz，2H)，6.90(d，J＝8.8Hz，1H)，3.74(s，3H)。
The bromo-2-chloro-phenyl-of embodiment 5:(5-) synthesis of (4-fluorophenyl) ketone
Chloro-for 2-5-bromo-benzoic acid (100g, 0.42mol) is mixed in methylene dichloride (500mL), drips oxalyl chloride (107mL, 1.27mol) and DMF (2mL).At room temperature stir after 2 hours and excessive oxalyl chloride underpressure distillation removed, resistates is dissolved in fluorobenzene (500mL), adds aluminum chloride (62g, 0.46mol) at 10 DEG C.Reaction solution is poured in frozen water (2L) after reacting at 75 DEG C and spending the night, add concentrated hydrochloric acid (400mL), aqueous phase ethyl acetate (500mL × 3) extracts, merge organic phase with 5% aqueous sodium hydroxide solution, 2M hydrochloric acid soln and saturated common salt water washing, anhydrous sodium sulfate drying, vacuum rotary steam obtains (the bromo-2-chloro-phenyl-of 5-) (4-fluorophenyl) ketone (104g, productive rate: 78%) be off-white color solid.
1H NMR(400MHz，CDCl 3)：δ7.84-7.87(m，2H)，7.58(dd，J＝8.8，2.4Hz，1H)，7.52(d，J＝2.4Hz，1H)7.36(d，J＝8.8Hz，1H)，7.18(t，J＝8.4Hz，2H)。
By embodiment 5 synthetic method synthetic example 6 ~ 8 compound
Embodiment 6:(3-bromophenyl) (4-fluorophenyl) ketone
m/z：[M+H] +278.9
The bromo-2-of embodiment 7:4-(4-fluoro benzophenone base) cyanophenyl
1H NMR(400MHz，CDCl 3)：δ7.82-7.85(m，3H)，7.66(dd，J＝8.0，1.6Hz，1H)，7.63(d，J＝1.6Hz，1H)，7.20(t，J＝8.4Hz，2H)。
The bromo-2-aminomethyl phenyl of embodiment 8:(5-) (4-fluorophenyl) ketone
m/z：[M+H] +292.9
Embodiment 9: the synthesis of cumarone-2-base methyl alcohol
Step 1: cumarone-2-formaldehyde
Under-78 DEG C of conditions; to the cumarone (10g of nitrogen protection; hexane solution (the 2.5M of n-Butyl Lithium is dripped in dry tetrahydrofuran (100mL) solution 84.7mmol); 33.9mL; 84.7mmol), reaction system keeps-78 DEG C to stir 0.5 hour, drips N; dinethylformamide (19.6mL, 254mmol).Reaction system keeps-78 DEG C to stir 1 hour, add saturated aqueous ammonium chloride (20mL) cancellation, add ethyl acetate (300mL) and water (100mL), layering, aqueous phase ethyl acetate (50mL) extracts, merge organic phase, with water, saturated common salt water washing, anhydrous sodium sulfate drying.Vacuum rotary steam removes desolventizing, crude product silica gel column chromatography (sherwood oil: ethyl acetate=10: 1) purifying obtains cumarone-2-formaldehyde (7.5g, productive rate: 61%) be yellow oil.
1H NMR(400MHz，CDCl 3)：δ9.90(s，1H)，7.78(d，J＝8.8Hz，1H)，7.63(d，J＝8.2Hz，1H)，7.59(s，1H)，7.53-7.57(m，1H)，7.35-7.39(m，1H)。
Step 2: cumarone-2-base methyl alcohol
Under condition of ice bath, add sodium borohydride (1.55g) in methyl alcohol (40mL) solution of cumarone-2-formaldehyde (4.0g, 27.4mmol) prepared to step 1 in batches, stir 1 hour.Add ethyl acetate (100mL) dilution, organic phase washed with water (50mL × 3) and saturated common salt water washing, use anhydrous sodium sulfate drying.Vacuum rotary steam removes desolventizing and obtains cumarone-2-base methyl alcohol (4.0g, productive rate: 98%) be yellow oily liquid. 1H NMR(400MHz，CDCl 3)：δ7.57-7.59(m，1H)，7.48-7.50(m，1H)，7.23-7.33(m，2H)，6.66(s，1H)，4.78(s，2H)，2.03(brs，1H)。
Embodiment 10:(3,3-dimethyl-1,3-dihydroisobenzofuran-1-base) synthesis of methyl alcohol
The bromo-2-vinyl benzene of step 1:1-
Under condition of ice bath, the hexane solution (2.5M, 18mL, 45mmol) of n-Butyl Lithium is added drop-wise in tetrahydrofuran (THF) (50mL) mixed solution of methyltriphenylphospbromide bromide phosphorus (16.2g, 45mmol).Reaction system stirs 1 hour at 0 DEG C, and dissolution of solid adds 2-bromobenzaldehyde (8.0g, 43mmol) in reaction system, and reaction system stirs 1 hour at 0 DEG C.Poured into by reaction mixture in water (20mL), aqueous layer with ethyl acetate extracts, and merges organic phase, anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, and crude product is by silica gel column chromatography (sherwood oil: ethyl acetate=10: 1) purifying obtains the bromo-2-vinyl benzene of 1-(5.2g, yield: 65%) be yellow oil.
Step 2:2-(2-bromophenyl) oxyethane
Under condition of ice bath, by metachloroperbenzoic acid (2.0g, 10.9mmol) join in methylene dichloride (20mL) mixed solution of the bromo-2-vinyl benzene (3.0g, 13.1mmol) of 1-that step 1 prepares, reaction system stirs 2 hours at 0 DEG C in batches.Add saturated sodium bicarbonate aqueous solution (30mL) cancellation reaction, aqueous layer with ethyl acetate (50mL × 3) extracts, and merges organic phase, anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, and crude product thin layer prepares plate (sherwood oil: ethyl acetate=10: 1) purifying obtains 2-(2-bromophenyl) oxyethane (1.2g, yield: 54%) be yellow oil.
Step 3:(3,3-dimethyl-1,3-dihydroisobenzofuran-1-base) methyl alcohol
Under-70 DEG C of conditions, to 2-(2-bromophenyl) oxyethane (930mg that step 2 prepares, the Skellysolve A solution (1.3M, 7.1mL, 9.34mmol) of tert-butyl lithium is dripped in tetrahydrofuran (THF) (10mL) solution 4.67mmol).Reaction system drips acetone (542mg, 9.34mmol) after stirring 10 minutes, stirs 1 hour after temperature slowly rises to 15 DEG C.Poured into by reaction mixture in water (20mL), aqueous layer with ethyl acetate extracts, and merges organic phase, anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, crude product thin layer prepares plate (sherwood oil: ethyl acetate=1: 1) purifying obtains (3,3-dimethyl-1,3-dihydroisobenzofuran-1-base) methyl alcohol (670mg, yield: 80%) be pale yellow oil.
1H NMR(400MHz，CDCl 3)：δ7.31-7.34(m，2H)，7.15-7.21(m，2H)，5.31-5.34(m，1H)，3.95-4.00(m，1H)，3.77-3.83(m，1H)，1.60(s，3H)，1.52(s，3H)。
Embodiment 11:(1,3-dihydroisobenzofuran-1-base) synthesis of methyl alcohol
Step 1:2-vinylbenzaldehyde
Under-78 DEG C of conditions, by n-Butyl Lithium (2.5M, 7.2mL, 18.0mmol) be added drop-wise to the bromo-2-vinyl benzene of the 1-(3g that embodiment 10 step 1 prepares, in tetrahydrofuran (THF) (20mL) solution 16.4mmol), keep-70 DEG C to stir after 1 hour and drip DMF (1.8g, 24.6mmol), reaction system stirs 30 minutes after rising to room temperature gradually.Reaction system is poured in water (20mL), with ethyl acetate (20mL × 2) extraction, merges organic phase, anhydrous sodium sulfate drying.Underpressure distillation removes desolventizing and obtains 2-vinylbenzaldehyde (2.0g, yield: 91%).
Step 2:(2-vinyl benzene) methyl alcohol
2-vinylbenzaldehyde (the 2.0g that step 1 is prepared, 15.3mmol) be dissolved in the mixed solvent of tetrahydrofuran (THF) (14mL) and methyl alcohol (14mL), add sodium borohydride (1.15g under 0 DEG C of condition in batches, 30.3mmol), reaction system stirring at room temperature 2 hours.Add ethyl acetate (50mL), with saturated aqueous ammonium chloride and saturated common salt water washing, anhydrous sodium sulfate drying.Underpressure distillation removes desolventizing and obtains (2-vinyl benzene) methyl alcohol (2.0g, yield: 100%).
Step 3:(1,3-dihydroisobenzofuran-1-base) methyl alcohol
(2-vinyl benzene) methyl alcohol (1.0g that step 2 is prepared, 7.45mmol) be dissolved in methylene dichloride (10mL), metachloroperbenzoic acid (1.82g is added under 0 DEG C of condition, 8.94mmol), reaction system room temperature for overnight, with methylene dichloride (30mL) dilution, use saturated sodium bicarbonate aqueous solution and saturated common salt water washing respectively, anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, and crude product thin layer prepares plate (sherwood oil: ethyl acetate=4: 1) purifying obtains (1,3-dihydroisobenzofuran-1-base) methyl alcohol (260mg, yield: 23%) be yellow oil.
m/z：[M+H] +151.1
Embodiment 12:(1-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methyl alcohol
The bromo-2-of step 1:1-(2-propenyl) benzene
By potassium tert.-butoxide (21.1g under nitrogen protection; 0.188mol) be suspended in tetrahydrofuran (THF) (250mL), ice bath is cooled to system temperature lower than 10 DEG C, adds methyltriphenylphospbromide bromide phosphorus (67.3g in system in batches; 0.188mol); finish insulation reaction 2 hours, be cooled to 0 DEG C, in system, drip 1-(2-bromophenyl) ethyl ketone (25g; 0.126mol); keep system temperature to be less than 5 DEG C, finish, stir 16 hours.Water (250mL) is added in system.Extract by ethyl acetate (200mL × 3), merge organic phase, wash with saturated brine, anhydrous sodium sulfate drying, filter, underpressure distillation is except desolventizing, and crude product obtains the bromo-2-of 1-(2-propenyl) benzene (18.1g, yield: 71%) be weak yellow liquid by silica gel column chromatography (sherwood oil) purifying.
1H NMR(400MHz，CDCl 3)：δ7.58(dd，J 1＝1.2Hz，J 1＝7.6Hz，1H)，7.27-7.31(m，1H)，7.21-7.23(m，1H)，7.12-7.16(m，1H)，5.26(m，1H)，4.97(m，1H)，2.13(s，3H)。
Step 2:2-(2-propenyl) phenyl aldehyde
The bromo-2-of the 1-under nitrogen protection, step 1 prepared (2-propenyl) benzene (16g; 81mmol) be dissolved in tetrahydrofuran (THF) (160mL); be cooled to about-65 DEG C; n-Butyl Lithium (2.5M is slowly added dropwise in system; 35.7mL; 89.0mmol); keep system temperature lower than-60 DEG C; finish, insulation reaction 30 minutes, is added dropwise to N in system; dinethylformamide (8.9g; 122mmol), finish, reaction system rises to stirring at room temperature 2 hours.Add saturated aqueous ammonium chloride cancellation reaction, ethyl acetate (200mL × 2) extracts, organic phase merges, use saturated common salt water washing, anhydrous sodium sulfate drying, it is weak yellow liquid that filtering and concentrating obtains 2-(2-propenyl) phenyl aldehyde (11.8g, yield 99%).
Step 3:(2-(2-propenyl) benzene) methyl alcohol
2-(2-propenyl) phenyl aldehyde (11.8g that step 2 is prepared, 80mmol) be dissolved in methyl alcohol (95mL), ice bath is cooled to 0 ~ 5 DEG C, add sodium borohydride (3.67g in batches, 97mmol), finish, insulation reaction 2 hours, add 2M hydrochloric acid soln cancellation reaction, be extracted with ethyl acetate (100mL × 3) extraction three times, merge organic phase, saturated aqueous common salt (100mL) washs, anhydrous sodium sulfate drying, filtering and concentrating is also with silica gel column chromatography (sherwood oil: ethyl acetate=15: 1) purifying obtains (2-(2-propenyl) benzene) methyl alcohol (8.8g, yield 74%) be light yellow liquid.
1HNMR(CDCl 3，400Hz)：δ7.47-7.49(m，1H)，7.28-7.33(m，2H)，7.19-7.21(m，1H)，5.27(m，1H)，4.93(m，1H)，4.72(s，2H)，2.10(s，3H)。
Step 4:1-(chloromethyl)-2-(2-propenyl) benzene
(2-(2-propenyl) benzene) methyl alcohol (7.0g that step 3 is prepared, 47.2mmol) be dissolved in methylene dichloride (70mL), ice bath is cooled to 0 ~ 5 DEG C, sulfur oxychloride (6.2g is dripped in system, 52.3mmol), finish, insulation reaction 1 hour, add saturated sodium bicarbonate aqueous solution (20mL), extract with methylene dichloride (50mL × 3), merge organic phase, saturated aqueous common salt (100mL) washs, anhydrous sodium sulfate drying, filtering and concentrating obtains 1-(chloromethyl)-2-(2-propenyl) benzene (7.5g, yield 95%) be light yellow liquid.
Step 5:(1-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methyl alcohol
1-(chloromethyl)-2-(2-propenyl) benzene (7.5g under nitrogen protection, step 4 prepared, 45mmol) be dissolved in acetone (75mL) with the mixed solvent of water (18.5mL), ice bath is cooled to 0 ~ 5 DEG C, N-methylmorpholine-N-oxide compound (15.8g is added successively in system, 135mmol) with perosmic anhydride (0.04g, 0.02mmo1), finish, room temperature reaction 16 hours, add saturated sodium sulfite cancellation reaction, stir 30 minutes, extract by ethyl acetate (100mL × 3), merge organic phase, saturated aqueous common salt (100mL) washs, anhydrous sodium sulfate drying, filtering and concentrating is also with silica gel column chromatography (sherwood oil: ethyl acetate=5: 1) purifying obtains (1-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methyl alcohol (3.7g, yield 50%) be colourless liquid.
1HNMR(CDCl 3，400Hz)：δ7.28-7.31(m，2H)，7.22-7.24(m，1H)，7.15-7.17(m，1H)，5.14(s，2H)，3.68-3.74(m，2H)，2.49(br，1H)，1.50(s，3H)。
Embodiment 13:(S) synthesis of-(1,3-dihydroisobenzofuran-1-base) methyl alcohol
Step one: 2-vinylacetic acid benzyl ester
By (2-ethenylphenyl) methyl alcohol (3.0g, 22.4mmol) with triethylamine (4.5g, 44.7mmol) be dissolved in methylene dichloride (20mL), add diacetyl oxide (4.6g, 44.7mmol) with catalytic amount N, N-lutidine (50mg), reaction system stirred overnight at room temperature, add methylene dichloride (30mL) dilution, use water and saturated common salt water washing successively, anhydrous sodium sulfate drying, filtering and concentrating, it is pale yellow oil that purification by silica gel column chromatography obtains 2-vinylacetic acid benzyl ester (3.9g, productive rate 96%).
Step 2: (R)-2-(1,2-di-alcohol) jasmal
AD-mix-β (4.5g) is mixed in the trimethyl carbinol (10mL) and water (10mL), be cooled to 0 DEG C and drip 2-vinylacetic acid benzyl ester (500mg, 3.5mmol), reaction system maintains 0 DEG C of stirring and spends the night, reaction solution S-WAT cancellation, aqueous phase is extracted with ethyl acetate (30mL × 3), merge organic phase saturated common salt water washing, anhydrous sodium sulfate drying, filtering and concentrating, plate purifying prepared by silica gel, and to obtain (R)-2-(1,2-di-alcohol) jasmal (432mg, productive rate 58%) be weak yellow liquid.
1H NMR(400MHz，CDCl 3)：δ7.50-7.58(m，1H)，7.32-7.42(m，3H)，5.18(dd，J＝12.0Hz，2H)，5.08(m，1H)，3.66-3.78(m，2H)，3.01(s，1H)，2.54(s，1H)，2.14(s，3H)。
Step 3: (R)-2-(1-hydroxyl-2-((triisopropylsilyl oxygen base) ethyl) jasmal
By (R)-2-(1,2-di-alcohol) jasmal (200mg, 0.95mmol) with imidazoles (77mg, 1.14mmol) be dissolved in methylene dichloride (2ml), be cooled to 0 DEG C, drip tri isopropyl chlorosilane (201mg, 1.05mmol), reaction system rises to ambient temperature overnight, the concentrated purification by silica gel column chromatography of reaction solution (sherwood oil: ethyl acetate=4: (1-hydroxyl-2-((triisopropylsilyl oxygen base) ethyl) jasmal (128mg) is pale yellow oily liquid body 1) to obtain (R)-2-.
1H NMR(400MHz，CDCl 3)：δ7.61-7.63(m，1H)，7.32-7.43(m，3H)，5.17(dd，J＝12.0Hz，2H)，5.08(m，1H)，3.88-3.92(m，1H)，3.65-3.69(m，1H)，3.20(s，1H)，1.14(s，12H)，1.12(s，6H)。
Step 4: (R)-2-(1-(methylsulfonyl oxygen base)-2-((triisopropylsilyl oxygen base) ethyl) jasmal
(R)-2-(1-hydroxyl-2-((triisopropylsilyl oxygen base) ethyl) jasmal (60mg, 0.16mmol) be dissolved in methylene dichloride (2mL), add triethylamine (50mg, 0.49mmol), then methylsulfonyl chloride (37mg, 0.32mmol are dissolved in 0.5mL methylene dichloride) is dripped.Reaction stirring at room temperature 2 hours, add methylene chloride dilution, and use 2N hydrochloric acid soln and saturated common salt water washing respectively, anhydrous sodium sulfate drying, filtering and concentrating obtains resistates and is directly used in the next step.
Step 5: (S)-((the different hydrogen cumarone of 1,3-bis--1-base) methoxyl group) tri isopropyl silane
By step 4 gained compound (73mg, 0.16mmol) be dissolved in dehydrated alcohol (1mL), add NaOH (13mg, 0.32mmol is dissolved in 0.5mL ethanol), reaction stirring at room temperature 1 hour, reaction solution is directly concentrated that plate purifying (sherwood oil: ethyl acetate=10: 1) obtain compound (S)-((1 prepared by thick product silica gel, the different hydrogen cumarone of 3-bis--1-base) methoxyl group) tri isopropyl silane (33mg, 2 step productive rates 66%) is colorless oil.
1H NMR(400MHz，CDCl 3)：δ7.22-7.36(m，4H)，5.29(t，J＝5.2Hz，1H)，5.10-5.20(m，2H)，3.98(dd，J＝5.2，10.0Hz，1H)，3.90(dd，J＝5.2，10.0Hz，1H)，1.02-1.16(m，21H)。
Step 6: (S)-(1,3-dihydroisobenzofuran-1-base) methyl alcohol
By (S)-((1, the different hydrogen cumarone of 3-bis--1-base) methoxyl group) tri isopropyl silane (33mg, 0.11mmol) be dissolved in tetrahydrofuran (THF) (2mL), add tetrabutyl ammonium fluoride (14mg, 0.06mmol), reaction stirring at room temperature 5 hours, reaction solution directly prepares plate (sherwood oil: ethyl acetate=3: 1) purifying obtains (S)-(1 with silica gel, 3-dihydroisobenzofuran-1-base) methyl alcohol (5mg, productive rate 30%, e.e. value: 97.2%) be colorless oil.
1H NMR(400MHz，CDCl 3)：δ7.23-7.35(m，4H)，5.35-5.36(m，1H)，5.13-5.22(m，2H)，3.97(dd，J＝3.2，12.0Hz，1H)，3.82(dd，J＝5.6，11.6Hz，1H)。
[α] D 25＝22.11(C＝0.502，MeOH)
Embodiment 14:(R) synthesis of-(1,3-dihydroisobenzofuran-1-base) methyl alcohol
Step one: 1-(chloromethyl)-2-vinyl benzene
(2-vinyl benzene) methyl alcohol (3.10g, 23.1mmol) be dissolved in methylene dichloride (30mL), be cooled to 0 DEG C, be added dropwise to thionyl chloride (2.01mL, 27.7mmol), reaction stirred overnight at room temperature, add methylene chloride (50mL) dilution, use water (30mL x 2), saturated sodium bicarbonate solution (20mL x 1) and saturated common salt water washing successively, anhydrous sodium sulfate drying, it is colorless oil that filtering and concentrating obtains 1-(chloromethyl)-2-vinyl benzene (2.92g, productive rate 83%), is directly used in the next step.
Step 2: (R)-1-(2-(chloromethyl) phenyl) ethyl-1,2-glycol
AD-mix-β (34.6g) is dissolved in butanol/water (260mL, 1: 1) in, be cooled to 0 DEG C, be added dropwise to compound 1-(chloromethyl)-2-vinyl benzene (4.0g, 26.2mmol, be dissolved in the 5mL trimethyl carbinol), react 0 DEG C to stir 2 hours, add sodium sulphite anhydrous 99.3 (39g) cancellation reaction, ethyl acetate (100mL × 2) extracts, organic phase merges, and with water and saturated common salt water washing, anhydrous sodium sulfate drying, filtering and concentrating obtains (R)-1-(2-(chloromethyl) phenyl) ethyl-1, 2-glycol (3.5g, productive rate 72%) be faint yellow solid.
1H NMR(400MHz，CDCl 3)：δ7.58(d，J＝7.6Hz，1H)，7.28-7.43(m，3H)，5.21(dd，J＝3.2，8.4Hz，1H)，4.72(s，2H)，3.86(dd，J＝3.2，11.2Hz，1H)，3.76(dd，J＝8.8，11.2Hz，1H)，2.70(br，1H)，2.23(br，1H)。
Step 3: (R)-(1,3-dihydroisobenzofuran-1-base) methyl alcohol
(R)-1-(2-(chloromethyl) phenyl) ethyl-1, 2-glycol (3.5g, 18.8mmol) be dissolved in tetrahydrofuran (THF) (35mL), be cooled to 0 DEG C, add sodium hydride (1.65g in batches, 60%, 41.3mmol), finish, room temperature reaction 2 hours, add frozen water cancellation, be extracted with ethyl acetate (50mL x2), merge organic phase, and with water and saturated common salt water washing, anhydrous sodium sulfate drying, filtering and concentrating, (R)-(1 is obtained with purification by silica gel column chromatography, 3-dihydroisobenzofuran-1-base) methyl alcohol (1.8g, productive rate 64%, e.e. be worth: 97%) be light yellow liquid.
1H NMR(400MHz，CDCl 3)：δ7.24-7.36(m，4H)，5.35-5.36(m，1H)，5.14-5.23(m，2H)，3.95(dd，J＝3.2，12Hz，1H)，3.82(dd，J＝5.6，11.6Hz，1H)。
[α] D 25＝-24.14(C＝0.505，MeOH)
Embodiment 15:(S)-(5-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methyl alcohol
By the synthetic method of embodiment 14 compound, with AD-mix-α for chiral raw material preparation (S)-(5-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methyl alcohol.
1H NMR(400MHz，CDCl 3)：δ7.13(s，1H)，7.08(s，2H)，5.52(s，1H)，5.09-5.18(m，2H)，3.95(dd，J＝3.2，12.0Hz，1H)，3.79(dd，J＝5.6，12.0Hz，1H)，2.39(s，3H)1.78(s，1H)。
Embodiment 16:(S)-(fluoro-1, the 3-dihydroisobenzofuran-1-base of 5-) methyl alcohol
By the synthetic method of embodiment 14 compound, with AD-mix-α for chiral raw material preparation (S)-(fluoro-1, the 3-dihydroisobenzofuran-1-base of 5-) methyl alcohol.
1H NMR(400MHz，CDCl 3)：δ7.19(dd，J＝4.8，8.4Hz，1H)，6.95-7.03(m，2H)，5.30(s，1H)，5.09-5.19(m，2H)，3.94(dd，J＝3.2，12.0Hz，1H)，3.79(dd，J＝6.0，12.0Hz，1H)，1.92(s，1H)。
The synthesis of embodiment 17:2-allyl group-3-fluorophenol and 2-allyl group-5-fluorophenol
Step 1:3-fluorophenyl allyl ether
3-fluorophenol (20g, 178mmol) is dissolved in acetone (200mL), adds cesium carbonate solid (87g, 268mmol), under ice bath, drip bromopropylene (19mL, 214mmol).Finish and rise to stirring at room temperature 4 hours, filtering solids, filtrate is diluted by ethyl acetate (200mL), and organic phase uses water and saturated common salt water washing respectively, uses anhydrous sodium sulfate drying.Underpressure distillation removes desolventizing and obtains 3-fluorophenyl allyl ether (25g, productive rate: 92%) be brown oil liquid.Step 2:2-allyl group-3-fluorophenol and 2-allyl group-5-fluorophenol
3-fluorophenyl allyl ether (the 10g that step 1 prepares, 66mmol) and N, N-Diethyl Aniline (15mL) stirs 2 hours under 225 DEG C of conditions, be cooled to room temperature, reaction solution ethyl acetate (250mL) is diluted, organic phase 6N hydrochloric acid soln (100mL) and saturated common salt water washing, use anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, residue purification by silica gel column chromatography (petrol ether/ethyl acetate=100: 1 ~ 20: 1) obtain 2-allyl group-5-fluorophenol (4.0g obtained, productive rate: 40%) be pale yellow oil, 2-allyl group-3-fluorophenol (2.2g, productive rate 22%) is pale yellow oil.
2-allyl group-5-fluorophenol
1H NMR(400MHz，CDCl 3)：δ7.06(dd，J＝6.8，8.0Hz，1H)，6.58-6.65(m，2H)，5.97-6.05(m，1H)；5.32(br s，1H)，5.16-5.21(m，1H)，3.39(d，J＝6.0Hz，2H).
2-allyl group-3-fluorophenol
1H NMR(400MHz，CDCl 3)：δ7.07-7.12(m，1H)，6.64-6.71(m，2H)，5.96-6.06(m，1H)，5.16-5.21(m，2H)，5.14(t，J＝2.0Hz，1H)，3.48(dd，J＝1.2，5.6Hz，2H).
The synthesis of embodiment 18:2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl) fluoro-2, the 3-Dihydrobenzofuranes of-7-
Step 1:2-fluorophenyl allyl ether
Adjacent fluorophenol (21g, 187mmol) is dissolved in acetone (210mL), adds cesium carbonate solid (91.5g, 281mmol), under ice bath, drip bromopropylene (27.1g, 225mmol).Finish and rise to stirred overnight at room temperature, filtering solids, filter cake ethyl acetate (400mL) washing, filtrate uses 10% aqueous sodium hydroxide solution (100mL), water and saturated common salt water washing respectively, uses anhydrous sodium sulfate drying.To filter and vacuum rotary steam obtains 2-fluorophenyl allyl ether (25g, productive rate: 88%) be yellow oily liquid except desolventizing.
Step 2:2-allyl group-6-fluorophenol
2-fluorophenyl allyl ether (the 5.0g that step 1 is prepared, 32mmol) be dissolved in N-Methyl pyrrolidone (50mL), be heated to 225 DEG C stir 3 hours, be cooled to room temperature, add ethyl acetate (200mL) dilution, use water and saturated common salt water washing respectively, use anhydrous sodium sulfate drying.Filtration, vacuum rotary steam are except desolventizing, and (crude product can be directly used in next step reaction for 5.0g, productive rate: 100%) be yellow oil to obtain 2-allyl group-6-fluorophenol.
Fluoro-2, the 3-Dihydrobenzofuranes-2-bases of step 3:(7-) methyl alcohol
2-allyl group-6-fluorophenol step 2 prepared is dissolved in chloroform (5.0g, 32.9mmol), adds metachloroperbenzoic acid (7.6g, 32.9mmol, 75%).Reaction system reflux stirs 3 hours, is cooled to room temperature, and reaction solution saturated sodium bicarbonate, water and saturated common salt water washing, use anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, crude product purification by silica gel column chromatography (the sherwood oil: ethyl acetate=10: 1) obtain (7-fluoro-2 obtained, 3-Dihydrobenzofuranes-2-base) methyl alcohol (2.1g, productive rate: 38%) be pale yellow oil.
1H NMR(400MHz，CDCl 3)：δ6.90-6.97(m，2H)，6.78-6.83(m，1H)，5.00-5.06(m，1H)，3.93(dd，J＝3.2，12.4Hz，1H)，3.79(dd，J＝6.0，12.0Hz，1H)，3.31(dd，J＝9.2，15.6Hz，1H)，3.14(dd，J＝7.6，16.0Hz，1H)。
Step 4:(5-bromo-2-chloro-phenyl-) (4-((fluoro-2, the 3-Dihydrobenzofuranes-2-bases of 7-) methoxyl group) phenyl) ketone
Under condition of ice bath, to (the 7-fluoro-2 that step 3 prepares, 3-Dihydrobenzofuranes-2-base) methyl alcohol (708mg, 4.21mmol) with (the chloro-phenyl of the bromo-2-of 5-) (the fluoro-phenyl of 4-) ketone (1.10g, potassium tert.-butoxide (413mg, 3.68mmol) is added in DMF (10mL) solution 3.51mmol), reaction system becomes dark-brown, rises to stirred overnight at room temperature.Add water (50mL) cancellation reaction, reaction solution ethyl acetate (30mL × 3) extraction, merge organic phase, organic phase washed with water and saturated common salt water washing, use anhydrous sodium sulfate drying.Vacuum rotary steam is except desolventizing, the residue thin layer obtained prepares plate purifying (sherwood oil: ethyl acetate=3: 1) obtain (the chloro-phenyl of the bromo-2-of 5-) (4-((7-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) phenyl) ketone (770mg, productive rate: 48%) be colorless oil.
m/z：[M+H] +461.0
Step 5:2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl) fluoro-2, the 3-Dihydrobenzofuranes of-7-
To (the chloro-phenyl of the bromo-2-of 5-) (4-((7-fluoro-2 that step 4 prepares under ice bath, nitrogen protection condition; 3-Dihydrobenzofuranes-2-base) methoxyl group) phenyl) ketone (770mg; 1.67mmol) with chlorotriethyl silane (1.07mL; 6.67mmol) 1; boron trifluoride diethyl etherate (0.62mL is dripped in the mixed solution of 2-ethylene dichloride (3mL) and acetonitrile (3mL); 5mmol); finish, reaction system rises to rt while stirring overnight gradually.Add water (30mL) and ethyl acetate (50mL), layering, aqueous phase ethyl acetate (30mL) extraction, merges organic phase, organic phase saturated sodium bicarbonate aqueous solution (20mL × 2) and saturated common salt water washing, use anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, the residue thin layer obtained prepares plate purifying (sherwood oil: ethyl acetate=5: 1) obtain 2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl)-7-fluoro-2,3-Dihydrobenzofuranes (400mg, productive rate: 54%) be white solid.
1H NMR(400MHz，CDCl 3)：δ7.23-7.31(m，3H)，7.10(d，J＝11.2Hz，2H)，6.88-7.00(m，4H)，6.79-6.84(m，1H)，5.23-5.29(m，1H)，4.24(dd，J＝5.6，10.0Hz，1H)，4.14(dd，J＝5.2，10.0Hz，1H)，4.01(s，2H)，3.44(dd，J＝9.2，15.6Hz，1H)，3.27(dd，J＝7.2，15.6Hz，1H)。
The synthetic method of embodiment 18 compound is utilized to carry out synthetic example 19 ~ 41 compound
Embodiment 19:2-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-2,3-Dihydrobenzofuranes
1H NMR(400MHz，CDCl 3)：δ7.21-7.31(m，4H)，7.10-7.17(m，3H)，6.84-6.92(m，4H)，5.14-5.20(m，1H)，4.22(dd，J＝6.8，10Hz，1H)，4.10(dd，J＝4.4，10Hz，1H)，4.01(s，2H)，3.40(dd，J＝9.6，16Hz，1H)，3.16(dd，J＝7.2，15.6Hz，1H)。
Embodiment 20:2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl) fluoro-2, the 3-Dihydrobenzofuranes of-4-
m/z：[M+H] +447.1
Embodiment 21:2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl) fluoro-2, the 3-Dihydrobenzofuranes of-5-
m/z：[M+H] +447.1
Embodiment 22:2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl) fluoro-2, the 3-Dihydrobenzofuranes of-6-
m/z：[M+H] +447.1
The bromo-2-of embodiment 23:4-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) cyanophenyl
The bromo-2-of step 1:4-((4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) phenyl) (hydroxyl) methyl) cyanophenyl
By bromo-for 4-2-(4-((2; 3-Dihydrobenzofuranes-2-base) methoxyl group) benzoyl) cyanophenyl (620mg; 1.43mmol) be dissolved in tetrahydrofuran (THF) (6mL) and methyl alcohol (6mL); be cooled to 0 DEG C; slowly add sodium borohydride (81mg, 2.14mmol).Keeping 0 DEG C to react poured in water (20mL) by reaction soln after 30 minutes, add ethyl acetate (30mL), branch vibration layer, the bromo-2-of 4-((4-((2 is obtained after the dry concentrating under reduced pressure of organic layer, 3-Dihydrobenzofuranes-2-base) methoxyl group) phenyl) (hydroxyl) methyl) cyanophenyl (600mg, productive rate: 96%).
The bromo-2-of step 2:4-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) cyanophenyl
The bromo-2-of the 4-under nitrogen protection, step 1 prepared ((4-((2; 3-Dihydrobenzofuranes-2-base) methoxyl group) phenyl) (hydroxyl) methyl) cyanophenyl (600mg; 1.38mmol); triethyl silicane (479mg; 4.13mmol) be dissolved in acetonitrile (3.5mL) and methylene dichloride (3.5mL); boron trifluoride diethyl etherate (390mg, 2.75mmol) is dripped after being cooled to 0 DEG C.Reaction system keeps 0 DEG C to react after 2 hours, mixture is poured in water (20mL), aqueous layer with ethyl acetate (20mL x 3) extracts, merge organic phase, concentrate after anhydrous sodium sulfate drying and obtain crude product, crude product thin layer is prepared plate purifying and is obtained the bromo-2-of 4-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) cyanophenyl (275mg, yield: 48%).
1H NMR(400MHz，CDCl 3)：δ7.70(d，J＝8.0Hz，1H)，7.37(dd，J＝2.0，7.6Hz，1H)，7.32(d，J＝2.0Hz，1H)，7.22(d，J＝7.6Hz，1H)，7.15(t，J＝7.6Hz，1H)，7.10(d，J＝8.4Hz，2H)，6.83-6.93(m，4H)，5.14-5.21(m，1H)，4.23(dd，J＝6.4，10.0Hz，1H)，4.12(dd，J＝4.8，9.6Hz，1H)，4.06(s，2H)，3.41(dd，J＝9.6，15.6Hz，1H)，3.16(dd，J＝7.2，15.6Hz，1H)。
Embodiment 24:2-((4-(the bromo-2-methoxy-benzyl of 5-) phenoxy group) methyl)-2,3-Dihydrobenzofuranes
m/z：[M+H] +425.2
Embodiment 25:2-((4-(3-bromobenzyl) phenoxy group) methyl)-2,3-Dihydrobenzofuranes
1H NMR(400MHz，CDCl 3)：δ7.10-7.35(m，8H)，6.84-6.91(m，4H)，5.13-5.20(m，1H)，4.21(dd，J＝6.4，10Hz，1H)，4.10(dd，J＝4.4，10Hz，1H)，3.91(s，2H)，3.40(dd，J＝9.6，16Hz，1H)，3.15(m，J＝6.8，16Hz，1H)。
Embodiment 26:2-((4-(the bromo-2-methoxy-benzyl of 5-) phenyl) methyl) cumarone
m/z：[M+H] +422.9
Embodiment 27:3-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-1,1-dimethyl-1,3-dihydroisobenzofuran
m/z：[M+H] +456.8
Embodiment 28:1-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-1,3-dihydroisobenzofuran
1H NMR(400MHz，CDCl 3)：δ7.23-7.37(m，7H)，7.09-7.12(m，2H)，6.88-6.94(m，2H)，5.61(t，J＝5.2Hz，1H)，5.17-5.27(m，2H)，4.20(d，J＝5.6Hz，2H)，4.01(s，2H)。
Embodiment 29:1-((4-(the bromo-2-methyl-benzyl of 5-) phenoxy group) methyl)-1,3-dihydroisobenzofuran
1H NMR(400MHz，CDCl 3)：δ7.22-7.38(m，6H)，7.03(d，J＝8.8Hz，3H)，6.87-6.91(m，2H)，5.61(t，J＝5.2Hz，1H)，5.17-5.27(m，2H)，4.19(d，J＝5.2Hz，2H)，3.89(s，2H)，2.20(s，3H)。
Embodiment 30:(S)-1-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-1,3-dihydroisobenzofuran
m/z：[M+H] +429.4
Embodiment 31:2-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-5-methyl-2,3-Dihydrobenzofuranes
1H NMR(400MHz，CDCl 3)：δ7.24-7.32(m，3H)，7.03-7.14(m，3H)，6.89-6.96(m，3H)，6.42(d，J＝8.0Hz，1H)，5.12-5.18(m，1H)，4.21(dd，J＝6.8，10.0Hz，1H)，4.09(dd，J＝5.6，6.0Hz，1H)，4.02(s，2H)，3.36(dd，J＝9.2，16.0Hz，1H)，3.12(dd，7.2，16.0Hz，1H)，2.31(s，3H)。.
Embodiment 32:2-((4-(the bromo-2-methyl-benzyl of 5-) phenoxy group) methyl)-2,3-Dihydrobenzofuranes
1H NMR(400MHz，CDCl 3)：δ7.26-7.28(m，1H)，7.20-7.22(m，2H)，7.14(t，J＝8.0Hz，1H)，7.02-7.04(m，3H)，6.83-6.90(m，4H)，5.13-5.20(m，1H)，4.20(dd，J＝6.0，10.0Hz，1H)，4.10(dd，J＝4.8，10Hz，1H)，3.88(s，2H)，3.40(dd，J＝9.2，15.6Hz，1H)，3.15(dd，J＝6.8，15.6Hz，1H)，2.19(s，3H)。
Embodiment 33:(S)-1-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-5-methyl isophthalic acid, 3-dihydroisobenzofuran
m/z：[M+H] +443.2
Embodiment 34:(S)-1-((4-(5-bromo-2-chlorobenzyl) phenoxy group) methyl) fluoro-1, the 3-dihydroisobenzofuran of-5-
m/z：[M+H] +447.2
Embodiment 35:(S)-2-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-2,3-Dihydrobenzofuranes
m/z：[M+H] +429.2
Embodiment 36:(R)-1-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-1,3-dihydroisobenzofuran
1H NMR(400MHz，CDCl 3)：δ7.23-7.38(m，7H)，7.10-7.12(m，2H)，6.89-6.93(m，2H)，5.61(t，J＝5.2Hz，1H)，5.17-5.27(m，2H)，4.20(d，J＝6.0Hz，2H)，4.01(s，2H)。
Embodiment 37:(S)-2-((4-(the bromo-2-methyl-benzyl of 5-) phenoxy group) methyl)-2,3-Dihydrobenzofuranes
m/z：[M+H] +409.3
Embodiment 38:2-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-2-methyl-2,3-Dihydrobenzofuranes
By embodiment 18 compou nd synthesis method, bromo-for rare for bromine in step 1 third 3-2-methacrylic is substituted and prepares embodiment 38 compound.
1H NMR(400MHz，CDCl 3)：δ7.69(m，2H)，7.47(m，1H)，7.41(m，1H)，7.25(m，1H)，7.08(m，2H)，6.88(m，2H)，6.80(m，1H)，6.71(m，1H)，4.02(m，4H)，3.28(d，J＝16Hz，1H)，2.99(d，J＝16Hz，1H)，1.56(s，3H)。
Embodiment 39:2-((4-(the bromo-2-methyl-benzyl of 5-) phenoxy group) methyl)-2-methyl-2,3-Dihydrobenzofuranes
By embodiment 18 compou nd synthesis method, bromo-for rare for bromine in step 1 third 3-2-methacrylic is substituted and prepares embodiment 39 compound.
1H NMR(400MHz，CDCl 3)：δ7.26(m，1H)，7.12-7.21(m，3H)，7.01-7.04(m，3H)，6.83-6.89(m，3H)，6.80(m，1H)，3.97-4.02(m，2H)，3.88(s，2H)，3.37(d，J＝16Hz，1H)，3.04(d，J＝16Hz，1H)，2.19(s，3H)，1.63(s，3H)。
Embodiment 40:1-((4-(the bromo-2-chlorobenzyl of 5-) phenoxy group) methyl)-1-methyl isophthalic acid, 3-dihydroisobenzofuran
1H NMR(400MHz，CDCl 3)：δ7.07-7.35(m，7H)，7.08-7.10(m，2H)，6.85-6.89(m，2H)，5.19(s，2H)，4.01-4.16(m，2H)，3.99(s，2H)，1.68(s，3H)。
Embodiment 41:1-((4-(the bromo-2-methyl-benzyl of 5-) phenoxy group) methyl)-1-methyl isophthalic acid, 3-dihydroisobenzofuran
1H NMR(400MHz，CDCl 3)：δ7.32-7.34(m，3H)，7.23-7.30(m，2H)，7.22(d，J＝2.0Hz，1H)，7.01-7.05(m，3H)，6.84-6.86(m，2H)，5.20(s，2H)，4.06(dd，J 1＝9.2Hz，J 2＝17.6Hz，2H)，3.88(s，2H)，2.21(s，3H)，1.69(s，3H)。
Embodiment 42:(2S, 3R, 4R, 5S, 6S)-2-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl) synthesis of-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-34)
Step 1:(3R, 4S, 5S, 6R)-2-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol)-2-methoxyl group tetrahydrochysene-2H-pyrans-3,4,5-triol
2-((4-(5-bromo-2-chlorobenzyl) phenoxy group) the methyl)-7-fluoro-2 that embodiment 18 is prepared, 3-Dihydrobenzofuranes (400mg, 0.89mmol) be dissolved in dry tetrahydrofuran (THF) (2mL) and dry toluene (4mL),-78 DEG C, hexane solution (the 2.5M of n-Butyl Lithium is dripped under nitrogen protection, 0.35mL, 0.89mmol), during dropping, interior temperature is no more than-60 DEG C, finish reaction system to stir 30 minutes at-70 DEG C, drip 2, 3, 4, toluene (3mL) solution of 6-tetra--0-(TMS)-D-glucopyra saccharon, finish reaction system to stir 1 hour at-70 DEG C, add the methanol solution (103mg/5mL) of methylsulfonic acid, reaction system rises to stirred overnight at room temperature.Add saturated sodium bicarbonate solution (20mL) cancellation reaction, mixed solution ethyl acetate (30mL x 3) extraction, merge organic phase, organic phase saturated common salt water washing, uses anhydrous sodium sulfate drying.Vacuum rotary steam is except desolventizing, residue purification by silica gel column chromatography (ethanol/methylene=1: 20) obtain (3R obtained, 4S, 5S, 6R)-2-(the chloro-3-of 4-(4-((the fluoro-2,3 dihydro furan of 7--2-base) methoxyl group) benzyl) phenyl)-6-(methylol)-2-methoxyl group tetrahydrochysene-2H-pyrans-3,4,5-triol (330mg, productive rate: 66%) be white foam solid.
m/z：[M-H] -558.9
Step 2:(3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol
Under-15 DEG C of conditions, to (3R, 4S, 5S, 6R)-2-(the chloro-3-of 4-(4-((7-fluoro-2,3-dihydrofuran-2-base) methoxyl group) benzyl) phenyl)-6-(methylol)-2-methoxyl group tetrahydrochysene-2H-pyrans-3,4, boron trifluoride diethyl etherate (0.11mL is dripped in the methylene dichloride (2mL) of 5-triol (330mg, 0.59mmol) and chlorotriethyl silane (0.17mL, 1.18mmol) and the mixed solution of acetonitrile (2mL), 0.88mmol), finish, stir 2 hours, in this process, reaction system rises to 0 DEG C gradually.Add saturated sodium bicarbonate aqueous solution (20mL) cancellation reaction, reaction solution ethyl acetate (20mL x 3) extraction, merge organic phase, organic phase saturated common salt water washing, uses anhydrous sodium sulfate drying.Underpressure distillation removes desolventizing and obtains (3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (300mg, productive rate: 96%) be weak yellow foam shape solid.
m/z：[M+NH 4] +547.9
Step 3:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes)-2-base) methoxyl group) benzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
(the 3R that step 2 is prepared, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (330mg, 0.62mmol) be dissolved in methylene dichloride (3mL), add aceticanhydride (380mg successively, 3.73mmol), the 4-dimethylaminopyridine of pyridine (491mg, 6.22mmol) and catalytic amount.Stirring at room temperature was diluted by ethyl acetate (50mL) after 0.5 hour, use 2N hydrochloric acid soln (10mL), water (10mL), saturated sodium bicarbonate aqueous solution (10mL) and saturated common salt water washing respectively, use anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, crude product dehydrated alcohol recrystallization obtains (2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-((7-fluoro-2,3-Dihydrobenzofuranes)-2-base) methoxyl group) benzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester (210mg, productive rate: 48%) be white solid.
m/z：[M+H 2O] +716.2
Step 4:(2S, 3R, 4R, 5S, 6S)-2-(the chloro-3-of 4-(4-((fluoro-2, the 3-Dihydrobenzofuranes-2-bases of 7-) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol
To (the 2R that step 3 prepares, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-((7-fluoro-2, 3-Dihydrobenzofuranes) 2-yl) methoxyl group) benzyl) phenyl) tetrahydrochysene-2H-pyrans-3, 4, 5-tri-base triacetyl ester (210mg, sodium methylate (16mg is added in methyl alcohol (2mL) solution 0.30mmol), 0.30mmol), reaction solution stirred at ambient temperature neutralized with acetic acid after 2 hours, vacuum rotary steam is except desolventizing, crude product directly prepares plate (methylene dichloride: methyl alcohol=10: 1) purifying obtains (2S with thin layer, 3R, 4R, 5S, 6S)-2-(chloro-3 (the 4-((7-fluoro-2 of 4-, 3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3, 4, 5-triol (120mg, productive rate: 75%) be white solid.
m/z：[M+H 2O] +548.2
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.13(d，J＝8.8Hz，2H)，7.01(d，J＝7.6Hz，1H)，6.79-6.94(m，4H)，5.18-5.24(m，1H)，4.17-4.19(m，2H)，4.00-4.11(m，3H)，3.87-3.90(d，J＝11.6Hz，1H)，3.68-3.72(m，1H)，3.36-3.46(m，4H)，3.20-3.29(m，2H)。
Utilize the synthetic method of embodiment 42 compound, and corresponding bromide carrys out synthetic example 43 ~ 56 compound
Embodiment 43:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((4-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-31)
m/z：[M+NH 4] +547.9
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.09-7.14(m，3H)，6.84-6.88(m，2H)，6.57-6.61(m，2H)，5.16-5.21(m，1H)，4.10-4.20(m，5H)，3.87-3.90(m，1H)，3.68-3.73(m，1H)，3.28-3.49(m，5H)，3.18(dd，J＝7.2，16.0Hz，1H)。
Embodiment 44:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((5-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-32)
m/z：[M+NH 4] +547.9
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.12(d，J＝8.8Hz，2H)，6.85-6.88(m，1H)，6.79-6.87(m，3H)，6.66-6.70(m，1H)，5.09-5.16(m，1H)，4.00-4.14(m，5H)，3.87-3.90(m，1H)，3.68-3.73(m，1H)，3.28-3.49(m，5H)，3.16(dd，J＝7.2Hz，16.0Hz，1H)。
Embodiment 45:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((6-fluoro-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-33)
m/z：[M+NH 4] +547.9
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.12-7.17(m，3H)，6.84-6.87(m，2H)，6.49-6.59(m，2H)，5.14-5.19(m，1H)，4.00-4.18(m，5H)，3.87-3.91(m，1H)，3.68-3.73(m，1H)，3.28-3.49(m，5H)，3.11(dd，J＝7.2，16.0Hz，1H)。
Embodiment 46:2-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-((2S, 3R, 4R, 5S, 6R)-3,4,5-trihydroxy--6-(methylol) tetrahydrochysene-2H-pyrans-2-bases) cyanophenyl (Compound I-28)
m/z：[M+NH 4] +520.9
Embodiment 47:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-p-methoxy-phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-29)
m/z：[M+NH 4] +526.0
Embodiment 48:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-30)
m/z：[M+Na] +501.1
Embodiment 49:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2-ylmethoxy) benzyl)-4-p-methoxy-phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-5)
m/z：[M+NH 4] +523.9
Embodiment 50:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((3,3-dimethyl-1,3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-63)
m/z：[M+H] +540.9
Embodiment 51:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((5-methyl-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-85)
m/z：[M+Na] +549.1
Embodiment 52:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(((S)-1,3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-37)
m/z：[M+H] +513.2
I-37 retention time RT=5.00min (e.e. value: 100%) is recorded by chiral analysis method C.
Embodiment 53:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(((S)-5-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-101)
m/z：[M+H] +527.2
Embodiment 54:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(((S)-5-fluoro-1,3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-103)
m/z：[M+H] +531.1
Embodiment 55:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(((R)-1,3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-38)
m/z：[M+H] +513.2
I-38 retention time RT=3.23min (e.e. value: 100%) is recorded by chiral analysis condition C.
Embodiment 56:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(((S)-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-26)
m/z：[M+Na] +515.2
[α] D 25＝+44.1°(C＝0.204％，MeOH)
By chiral analysis method B 1record I-26 retention time RT=9.05min (e.e. value: 100%).
Embodiment 57: the preparation of Compound I-22, I-23 and I-24
(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-22)
According to the synthetic method synthetic compound I-22 of embodiment 42 compound, (chiral analysis condition A records RT=4.18min with chiral separation method A, I-22 (800mg) to be split as Compound I-23 (300mg), e.e. value: 99.0%) and Compound I-24 (170mg) (chiral analysis condition A records RT=4.91min, e.e. value: 98.9%).Embodiment 57 is split the I-23 obtained and embodiment 102 synthesize the I-23 that obtains according to 1: 1 ratio mix after, measure biased sample retention time RT=4.27min (e.e. value: 100%) by chiral analysis condition A; Embodiment 57 is split the I-24 obtained and embodiment 103 synthesize the I-24 that obtains according to 1: 1 ratio mix after, measure biased sample retention time RT=4.78min (e.e. value: 98%) by chiral analysis condition A.
Compound I-22
m/z：[M+Na] +535.2
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.20(dd，J＝0.4，7.6Hz，1H)，7.07-7.13(m，3H)，6.82-6.87(m，3H)，6.74(d，J＝8.0Hz，1H)，5.06-5.13(m，1H)，3.99-4.13(m，5H)，3.87-3.90(m，1H)，3.68-3.72(m，1H)，3.27-3.49(m，5H)，3.14(dd，J＝7.2，16.0Hz，1H)。
Compound I-23
[α] D 25＝+54.3°(C＝0.053％，MeOH)
Compound I-24
[α] D 25＝-20.0°(C＝0.051％，MeOH)
The preparation of embodiment 58:I-25, I-26 and I-27
(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-25)
According to embodiment 42 compou nd synthesis method synthetic compound I-25, with chiral separation method B, Compound I-25 (1.0g) is split as Compound I-26 (240mg) (by chiral analysis condition B 1record RT=9.28min, e.e. value: 100%) and Compound I-27 (205mg) (by chiral analysis condition B 1record RT=13.37min, e.e. value: 98.7%).Embodiment 58 is split the I-26 obtained and embodiment 56 synthesize the I-26 that obtains according to 1: 1 ratio mix after, by chiral analysis condition B 1measure biased sample retention time RT=9.68min (e.e. value: 100%)
Compound I-25
m/z：[M+Na] +515.2
1H NMR(400MHz，CD 3OD)：δ7.04-7.22(m，7H)，6.82-6.85(m，3H)，6.74(d，J＝7.6Hz，1H)，5.06-5.12(s，1H)，4.08-4.12(m，3H)，3.88-3.94(m，3H)，3.68-3.72(m，1H)3.35-3.50(m，5H)，3.11-3.16(m，1H)，2.20(s，3H)。
Compound I-26
[α] D 25＝+44.3°(C＝0.212％，MeOH)
Compound I-27
[α] D 25＝-20.9°(C＝0.210％，MeOH)
The preparation of embodiment 59:I-105, I-106 and I-107
(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((2-methyl-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-105)
According to the synthetic method synthetic compound I-105 of embodiment 42 compound, with chiral separation method C, Compound I-105 (72mg) is split as Compound I-106 (10mg) and (records RT=10.21min by chiral analysis condition D, e.e. value: 100%) and Compound I-107 (23mg) (record RT=6.27min by chiral analysis condition D, e.e. value: 100%).
Compound I-105
m/z：[M+NH 4] +544.2
1H NMR(400MHz，CDCl 3)：δ7.32(m，1H)，7.04-7.17(m，6H)，6.75-6.87(m，4H)，4.07(m，1H)，4.00(m，2H)，3.93(s，1H)，3.80(m，3H)，3.59-3.65(m，2H)，3.40(m，2H)，3.30(d，J＝16Hz，1H)，3.00(d，J＝16Hz，1H)，1.58(s，3H)。
The preparation of embodiment 60:I-109, I-110 and I-111
(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2-methyl-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-109)
According to the synthetic method synthetic compound I-109 of embodiment 42 compound, with chiral separation method C, Compound I-109 (115mg) is split as Compound I-110 (44mg) and (records RT=13.63min by chiral analysis condition D, e.e. value: 100%) and Compound I-111 (70mg) (record RT=7.97min by chiral analysis condition D, e.e. value: 100%).
Compound I-109
m/z：[M+NH 4] +524.2
1H NMR(400MHz，CDCl 3)：δ7.10-7.17(m，5H)，6.99(m，2H)，6.86(m，1H)，6.81(m，3H)，4.12(d，J＝9.2Hz，1H)，3.96(m，2H)，3.90(s，2H)，3.82(m，1H)，3.68(m，2H)，3.47(m，3H)，3.34(d，J＝16Hz，1H)，3.01(d，J＝16Hz，1H)，2.2(s，3H)，1.60(s，3H)。
Compound I-110
[α] D 25＝+4.52°(C＝0.051％，MeOH)
Compound I-111
[α] D 25＝-27.1°(C＝0.050％，MeOH)
The preparation of embodiment 61:I-36, I-37 and I-38
(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((1,3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-36)
According to the synthetic method synthetic compound I-36 of embodiment 42 compound, with chiral separation method A, Compound I-36 (800mg) is split as Compound I-38 (190mg) and (records RT=3.28min by chiral analysis condition C, e.e. value: 100%) and Compound I-37 (100mg) (record RT=4.89min by chiral analysis condition C, e.e. value: 100%).Embodiment 61 is split the I-37 obtained and embodiment 52 synthesize the I-37 that obtains according to 1: 1 ratio mix after, measure biased sample retention time RT=5.00min (e.e. value: 99.2%) by chiral analysis condition C.Embodiment 61 is split the I-38 obtained and embodiment 55 synthesize the I-38 that obtains according to 1: 1 ratio mix after, measure biased sample retention time RT=3.31min (e.e. value: 100%) by chiral analysis condition C.
Compound I-36
m/z：[M+H] +513.2
1H NMR(400MHz，CD 3OD)：δ7.28-7.39(m，7H)，7.11-7.13(m，2H)，6.84-6.87(m，2H)，5.54(s，1H)，5.10-5.22(m，2H)，4.23(dd，J＝4.0Hz，10.0Hz，1H)，4.00-4.16(m，4H)，3.87-3.90(m，1H)，3.68-3.72(m，1H)，3.28-3.49(m，4H)。
The preparation of embodiment 62:I-43, I-75 and I-76
(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((1,3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) 4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-43)
According to the synthetic method synthetic compound I-43 of embodiment 42 compound, with chiral separation method D, Compound I-43 (30mg) is split as Compound I-76 (10mg) and (records RT=3.32min by chiral analysis condition C, e.e. value: 100%) and Compound I-75 (6mg) (record RT=5.92min by chiral analysis condition C, e.e. value: 100%).
Compound I-43
m/z：[M+NH 4] +510.3
1HNMR(400MHz，CD 3OD)：δ7.29-7.39(m，4H)，7.12-7.22(m，3H)，7.04(d，J＝8.4Hz，2H)，6.84(m，J＝8.4Hz，2H)，5.53(s，1H)，5.10-5.21(m，2H)，4.08-4.24(m，3H)，3.88-3.94(m，3H)，3.68-3.72(m，1H)，3.37-3.50(m，4H)，2.20(s，3H)。
The preparation of embodiment 63:I-108, I-115 and I-116
(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((1-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-108)
According to the synthetic method synthetic compound I-108 of embodiment 42 compound, with chiral separation method E, Compound I-108 (900mg) is split as Compound I-115 (313mg) (by chiral analysis condition B 2record RT=7.99min, e.e. value: 99.3%) and Compound I-116 (369mg) (by chiral analysis condition B 2record RT=6.23min, e.e. value: 99.2%).
Compound I-108
m/z：[M+NH 4] +544.2
1H NMR(400MHz，CD 3OD)：δ7.12-7.30(m，7H)，6.99-7.01(m，2H)，6.71-6.73(m，2H)，5.06(d，J＝4.0Hz，10.8Hz，2H)，3.85-4.00(m，5H)，3.68-3.75(m，2H)，3.49-3.60(m，2H)，3.25-3.38(m，2H)，1.57(s，3H)。
The preparation of embodiment 64:I-112, I-113 and I-114
(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((1-methyl isophthalic acid, 3-dihydroisobenzofuran-1-base) methoxyl group) benzyl) 4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-112)
According to the synthetic method synthetic compound I-112 of embodiment 42 compound, with chiral separation method E, Compound I-112 (1.20g) is split as Compound I-113 (342mg) (by chiral analysis condition B 2record RT=12.00min, e.e. value: 98.1%) and Compound I-114 (414mg) (by chiral analysis condition B 2record RT=8.78min, e.e. value: 99.4%).
Compound I-112
m/z：[M+NH 4] +524.4
1HNMR(400MHz，CD 3OD)：δ7.22-7.33(m，4H)，7.09-7.18(m，3H)，6.97(d，J＝8.8Hz，2H)，6.78(d，J＝8.4Hz，2H)，5.14(d，J＝4.0Hz，2H)，4.09-4.16(m，1H)，3.96-4.02(m，2H)，3.77-3.88(m，4H)，3.62-3.70(m，2H)，3.42-3.52(m，2H)，2.19(s，3H)，1.63(s，3H)。
Method 2
The synthesis of the chloro-2-of the bromo-1-of embodiment 65:4-(4-ethoxy benzyl) benzene
The bromo-2-chloro-phenyl-of step 1:(5-) (4-ethoxyl phenenyl) ketone
By oxalyl chloride (8.1mL, 95.6mmol) and N, dinethylformamide (0.5mL) joins in methylene dichloride (50mL) mixture of the bromo-2-chloro-benzoic acid (15g, 63.7mmol) of 5-, room temperature for overnight.Underpressure distillation goes out desolventizing, is dissolved in by resistates in methylene dichloride (30mL), under ice bath cooling conditions, adds aluminum chloride solid (10.2g, 95.6mmol) in batches.Reaction system stirs 1 hour under condition of ice bath, reaction solution is directly poured on (100g) cancellation reaction in mixture of ice and water.With ethyl acetate (100 × 2) extraction mixture, organic phase 1M aqueous sodium hydroxide solution (100 × 2), water and saturated common salt water washing, use anhydrous sodium sulfate drying.Underpressure distillation except desolventizing, crude product dehydrated alcohol recrystallization obtains (the bromo-2-chloro-phenyl-of 5-) (4-ethoxyl phenenyl) ketone (20g, productive rate: 92%) be white solid.
The chloro-2-of the bromo-1-of step 2:4-(4-ethoxy benzyl) benzene
Under condition of ice bath, to (the bromo-2-chloro-phenyl-of 5-) (4-ethoxyl phenenyl) ketone (20g that step 1 prepares, triethyl silicane (21g is added in methylene dichloride (40mL) 58.9mmol) and acetonitrile (20mL) mixing solutions, 177mmol) with boron trifluoride diethyl etherate (15g, 106mmol).Reaction system rises to rt while stirring overnight gradually, add 7M potassium hydroxide aqueous solution (50mL) cancellation reaction, reaction solution methylene dichloride (200 × 2) extracts, merge organic phase, use saturated sodium bicarbonate aqueous solution (100 × 2), water and saturated common salt water washing respectively, use anhydrous sodium sulfate drying.Vacuum rotary steam is except desolventizing, and crude product dehydrated alcohol recrystallization obtains the chloro-2-of the bromo-1-of 4-(4-ethoxy benzyl) benzene (12g, productive rate: 60%) be white solid.
1H NMR(400MHz，CDCl 3)：δ7.24-7.31(m，3H)，7.11(d，J＝8.8Hz，2H)，6.86(d，J＝8.8Hz，2H)，4.04(q，J＝6.8Hz，2H)，4.01(s，2H)，1.43(t，J＝6.8Hz，3H)。
Utilize synthetic method synthetic example 66 ~ 70 compound of embodiment 65 compound
The bromo-2-of embodiment 66:4-(4-ethoxy benzyl)-1-methylbenzene
The bromo-2-of embodiment 67:4-(4-ethoxy benzyl)-1-fluorobenzene
1H NMR(400MHz，CDCl 3)：δ7.24-7.32(m，2H)，7.12(d，J＝8.2Hz，2H)，6.84-6.96(m，3H)，4.03(q，J＝6.8Hz，2H)，3.91(s，2H)，1.42(t，J＝6.8Hz，3H)。
The chloro-2-of the bromo-1-of embodiment 68:4-(the fluoro-4-methoxy-benzyl of 3-) benzene
m/z：[M+H] +331.1
The bromo-3-of embodiment 69:1-(4-ethoxy benzyl) benzene
1H NMR(400MHz，CDCl 3)：δ7.08-7.35(m，6H)，6.84-6.88(m，2H)，4.04(q，J＝6.8Hz，2H)，3.90(s，2H)，1.43(t，J＝6.8Hz，3H)。
The bromo-2-of embodiment 70:4-(4-ethoxy benzyl) cyanophenyl
m/z：[M+NH 4] +332.8
Embodiment 71:(2R, 3R, 4R, 5S, 6S) synthesis of-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
Step 1:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-ethoxy benzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-ethoxy benzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4, the synthesis of 5-tri-base triacetyl ester can reference example 42 or JMed.Chem.2008,57,1145-1149.
Step 2:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
Under-70 DEG C of conditions, to (the 2R that step 1 prepares, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-ethoxy benzyl) phenyl) tetrahydrochysene-2H-pyrans-3, 4, 5-tri-base triacetyl ester (700mg, boron tribromide (0.94mL is dripped in anhydrous methylene chloride solution (15mL) 1.21mmol), 9.71mmol), reaction system rises to-15 DEG C and stirs 1 hour, reaction solution ether (10mL) dilutes, stir 30 minutes, water (50mL) is used to dilute again, extract with methylene dichloride (100 × 2), merge organic phase anhydrous sodium sulfate drying, vacuum rotary steam removes desolventizing and obtains (2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3, 4, 5-tri-base triacetyl ester (650mg, productive rate: 98%) be white foam solid.
m/z：[M+NH 4] +565.9
Utilize synthetic method synthetic example 72 ~ 76 compound of embodiment 71 compound
Embodiment 72:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(3-(4-hydroxybenzyl)-4-aminomethyl phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
m/z：[M+NH 4] +545.9
Embodiment 73:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the fluoro-3-of-4-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
m/z：[M+Na] +555.2
Embodiment 74:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(the fluoro-4-hydroxybenzyl of 3-) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
m/z：[M+Na] +589.2
Embodiment 75:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(3-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
m/z：[M+NH 4] +531.9
Embodiment 76:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(4-cyano group-3-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
m/z：[M+NH 4] +556.9
Embodiment 77:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3, the synthesis of 4,5-triol
Step 1:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl) 6-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
Under condition of ice bath, to (the 2R that embodiment 72 prepares, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(4-methyl-3-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester (350mg, 0.66mmol), (2,3-Dihydrobenzofuranes-2--base) methyl alcohol (298mg, 1.99mmol) and triphenylphosphine (521mg, DIAD (0.39mL, 1.99mmol) is dripped in tetrahydrofuran (THF) (10mL) solution 1.99mmol).Reaction system rises to rt while stirring overnight gradually, and with ethyl acetate (50mL) dilution, organic phase washed with water and saturated common salt water washing, use anhydrous sodium sulfate drying.Underpressure distillation is except desolventizing, crude product thin layer is prepared plate (petrol ether/ethyl acetate=2/1) purifying and is obtained (2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl) 6-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester (60mg, productive rate: 14%) be white solid.
Step 2:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol
To the ((2R that step 1 prepares, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl) 6-(3-(4-((2, 3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl) tetrahydrochysene-2H-pyrans-3, 4, 5-tri-base triacetyl ester (60mg, sodium methylate (6mg is added in methyl alcohol (2mL) solution 0.09mmol), 0.10mmol), reaction solution stirred at ambient temperature neutralized with acetic acid after 2 hours, underpressure distillation desolventizes, crude product directly with thin layer prepare plate purify (methylene dichloride: methyl alcohol=10: 1) (2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2, 3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3, 4, 5-triol (34mg, productive rate: 76%) be white solid.
m/z：[M+Na] +515.2
Utilize the synthetic method of embodiment 77 compound, and corresponding alcohol carrys out synthetic example 78 ~ 79 compound
Embodiment 78:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl)-4-fluorophenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-21)
m/z：[M+Na] +519.2
1H NMR(400MHz，CD 3OD)：δ7.29-7.31(m，2H)，7.21(d，J＝7.2Hz，1H)，7.14(d，J＝8.8Hz，2H)，7.01-7.11(m，2H)，6.82-6.87(m，3H)，6.73(d，J＝8.8Hz，1H)，5.06-5.14(m，1H)，4.08-4.13(m，3H)，3.8-3.94(m，3H)，3.67-3.72(m，1H)，3.29-3.48(m，5H)，3.11-3.16(m，1H)。
Embodiment 79:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((2,3-Dihydrobenzofuranes-2-base) methoxyl group)-3-luorobenzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-12)
m/z：[M+Na] +553.0
Method 3
The synthesis of the fluoro-Benzaldehyde,2-hydroxy of embodiment 80:5-
To p-fluorophenol (25g, sodium hydroxide (39g is added in ethanol (100mL) 223mmol) and water (75mL) solution, water (200mL) solution 970mmol), mixing solutions is heated to 70 DEG C, chloroform (55mL is dripped in this solution, 691mmol), time for adding is about 2 hours.Finish, reaction system stirs at 70 DEG C spends the night, and reaction system is down to room temperature, add 6M hydrochloric acid soln (100mL) cancellation reaction, with methylene dichloride (250mL × 2) extraction, merge organic phase washed with water and saturated common salt water washing, use anhydrous sodium sulfate drying.Vacuum rotary steam removes desolventizing, crude product silica gel column chromatography (sherwood oil: ethyl acetate=25: 1) purifying obtains the fluoro-Benzaldehyde,2-hydroxy of 5-(6.5g, productive rate: 21%) be faint yellow solid.
1H NMR(400MHz，CDCl 3)：δ10.01(s，1H)，9.88(s，1H)，7.26-7.32(m，2H)，7.00(dd，J＝4.0，8.8Hz，1H)。
The synthesis of the fluoro-Benzaldehyde,2-hydroxy of embodiment 81:4-
To m fluorophenol (5mL, 55.3mmol), dry triethylamine (27mL) and paraformaldehyde (11g) is added in the mixture of the anhydrous acetonitrile (250mL) of Magnesium Chloride Anhydrous (14.2g, 149mmol).Reaction system is refluxed 5 hours, be cooled to room temperature, add hydrochloric acid soln (250mL) the cancellation reaction of 5%, mixed solution ethyl acetate (100 × 3) extracts, merge organic phase with 5% hydrochloric acid soln, water and saturated common salt water washing, use anhydrous sodium sulfate drying.Underpressure distillation removes desolventizing, crude product silica gel column chromatography (sherwood oil: ethyl acetate=50: 1 ~ 20: 1) purifying obtains the fluoro-Benzaldehyde,2-hydroxy of 4-(5g, productive rate: 65%) be faint yellow solid.
1H NMR(400MHz，CDCl 3)：δ11.37(d，J＝1.6，1H)，9.83(s，1H)，7.55-7.59(m，1H)，6.65-6.75(m，2H)。
The synthesis of the fluoro-6-hydroxy benzaldehyde of embodiment 82:2-
Step 1:2-(3-fluorophenyl) tetrahydrochysene-2H-pyrans
By m fluorophenol (5g, 44.6mmol), 3,4-dihydro-2H-pyrans (11.2mL, 134mmol) with trifluoroacetic acid (0.3mL, methylene dichloride (60mL) mixing solutions room temperature for overnight 4.0mmol), by reaction solution saturated sodium bicarbonate aqueous solution and saturated common salt water washing, use anhydrous sodium sulfate drying.Vacuum rotary steam removes desolventizing, crude product silica gel column chromatography (sherwood oil: ethyl acetate=100: 1 ~ 50: 1) purifying obtains 2-(3-fluorophenyl) tetrahydrochysene-2H-pyrans (7.5g, productive rate: 86%) be weak yellow liquid.
1H NMR(400MHz，CD 3OD)：δ7.20-7.26(m，1H)，6.80-6.86(m，2H)，6.68-6.73(m，1H)，5.42(t，J＝3.2Hz，1H)，3.88-3.94(m，1H)，3.62-3.66(m，1H)，1.99-2.02(m，1H)，1.86-1.90(m，2H)，1.57-1.74(m，3H)。
The fluoro-6-of step 2:2-((tetrahydrochysene-2H-pyrans-2-base) oxygen base) phenyl aldehyde
Under-78 DEG C of conditions, to 2-(3-fluorophenyl) tetrahydrochysene-2H-pyrans (6g that step 1 prepares, hexane solution (the 2.5M of n-Butyl Lithium is dripped in tetrahydrofuran solution (60mL) 30.6mmol), 14mL, 36.7mmol), reaction system stirs after 1.5 hours and drips N at-78 DEG C, dinethylformamide (6.7g, 91.3mmol), reaction system rises to stirred overnight at room temperature gradually, adds saturated aqueous ammonium chloride (50mL) cancellation reaction.Extract by ethyl acetate (100 × 2), merge organic phase anhydrous sodium sulfate drying, underpressure distillation removes desolventizing and obtains the fluoro-6-of 2-((tetrahydrochysene-2H-pyrans-2-base) oxygen base) phenyl aldehyde (6.5g, productive rate: 95%) be pale yellow oil.
The fluoro-6-hydroxy benzaldehyde of step 3:2-
The fluoro-6-of the 2-that step 2 is prepared ((tetrahydrochysene-2H-pyrans-2-base) oxygen base) phenyl aldehyde (6.5g, 29.0mmol) with tosic acid (2g, methylene dichloride (30mL) solution 11.6mmol) at room temperature stirs 5 hours, reaction solution methylene dichloride (100mL) dilutes, with saturated sodium bicarbonate aqueous solution and saturated common salt water washing, use anhydrous sodium sulfate drying.Vacuum rotary steam removes desolventizing, crude product silica gel column chromatography (sherwood oil: ethyl acetate=100: 1 ~ 20: 1) purifying obtains the fluoro-6-hydroxy benzaldehyde of 2-(3.0g, productive rate: 74%) be faint yellow solid.
1H NMR：(400MHz，CDCl 3)：δ11.49(s，1H)，10.29(s，1H)，7.46-7.52(m，1H)，6.79(d，J＝8.8Hz，1H)6.63-6.68(m，1H)。
The synthesis of embodiment 83:2-(chloromethyl)-5-Fluorobenzofur
Step 1:5-Fluorobenzofur-2-carboxylic acid, ethyl ester
Fluoro-Benzaldehyde,2-hydroxy (the 0.85g of the 5-that embodiment 80 is prepared, 6.07mmol) with salt of wormwood (1.68g, mixture 12.1mmol) is heated to 60 DEG C, ethyl bromoacetate (1.27g is added in this mixture, 7.58mmol), mixed system stirs 1 hour under 60 DEG C of conditions, is warming up to 60 DEG C, stirs 10 hours.With ethyl acetate (50mL) dilution, use saturated aqueous ammonium chloride and water washing respectively, use anhydrous sodium sulfate drying.Vacuum rotary steam is except desolventizing, and crude product thin layer prepares plate (sherwood oil: ethyl acetate=10: 1) purifying obtains 5-Fluorobenzofur-2-carboxylic acid, ethyl ester (450mg, productive rate: 36%) be faint yellow solid.
1H NMR(400MHz，CDCl 3)：δ7.56(dd，J＝4.0，8.8Hz，1H)，7.51(s，1H)，7.34-7.36(m，1H)，7.17-7.23(m，1H)，4.47(q，J＝7.2Hz，2H)，1.45(t，J＝7.2Hz，3H)。
Step 2:(5-Fluorobenzofur-2-base) methyl alcohol
Under condition of ice bath, in batches to the 5-Fluorobenzofur-2-carboxylic acid, ethyl ester (450mg that step 1 prepares, Lithium Aluminium Hydride (82mg is added in tetrahydrofuran (THF) (10mL) solution 2.16mmol), 2.16mmol), after reaction system reacts half an hour at 0 DEG C, add sal glauberi cancellation reaction, filter, filter cake ethyl acetate is washed, filtrate decompression distillation is except desolventizing, crude product thin layer prepares plate (sherwood oil: ethyl acetate=5: 1) purifying obtains (5-Fluorobenzofur-2-base) methyl alcohol (300mg, productive rate: 84%) be colourless oil liquid.
1H NMR(400MHz，CDCl 3)：δ7.39(dd，J＝4.0，8.8Hz，1H)，7.20-7.23(m，1H)，6.99-7.04(m，1H)，6.64(s，1H)，4.78(s，1H)，2.24(s，1H)。
Step 3:2-(chloromethyl)-5-Fluorobenzofur
Under condition of ice bath, to (5-Fluorobenzofur-2-base) methyl alcohol (150mg that step 2 prepares, thionyl chloride (0.13mL is dripped in methylene dichloride (5mL) solution 0.90mmol), 1.81mmol), reaction system rises to stirring at room temperature 1 hour, vacuum rotary steam except desolventizing obtain 2-(chloromethyl)-5-Fluorobenzofur (165mg, productive rate: 100%) be yellow oil, not purified be directly used in next step reaction.
Utilize synthetic method synthetic example 84 ~ 87 compound of embodiment 83 compound
Embodiment 84:2-(chloromethyl)-7-Fluorobenzofur
Embodiment 85:2-(chloromethyl)-6-Fluorobenzofur
Embodiment 86:2-(chloromethyl)-4-Fluorobenzofur
Embodiment 87:2-(chloromethyl) cumarone
The synthesis of embodiment 88:2-(2-bromotrifluoromethane)-2,3-Dihydrobenzofuranes
Step 1:2,3-Dihydrobenzofuranes-2-alcohol
By 2-chavicol (5g, 37.3mmol) be dissolved in the mixed solvent of tetrahydrofuran (THF) (25mL) and water (25mL), sodium periodate (23.9g is added under ice bath, 112mmol) with perosmic anhydride (95mg, 0.37mmol), reaction system room temperature for overnight.Add water (100mL) cancellation reaction, reaction solution ethyl acetate (100 × 2) extracts, merge organic phase anhydrous sodium sulfate drying, underpressure distillation is except desolventizing, crude product silica gel column chromatography (sherwood oil: ethyl acetate=20: 1 ~ 5: 1) purifying obtains 2,3-Dihydrobenzofuranes-2-alcohol (3g, productive rate: 60%) be pale yellow oil.
1H NMR(400MHz，CDCl 3)：δ7.16-7.28(m，2H)，6.86-6.95(m，2H)，6.07(d，J＝5.6，1H)，3.85(s，1H)，3.36-3.42(m，1H)，3.03-3.07(m，1H)。
Step 2:4-(2-hydroxy phenyl) methyl crotonate
Step 1 is prepared 2; 3-Dihydrobenzofuranes-2-alcohol (1.4g; 10.3mmol) with methoxycarbonyl methylene triphenylphosphine (3.4g; methyl tertiary butyl ether (30mL) mixture 10.3mmol) reacts and spends the night at 35 DEG C; filter the most of triphenylphosphine oxide generated; filtrate decompression is revolved and is steamed except desolventizing; obtain residue over silica gel column chromatography (sherwood oil: ethyl acetate=10: 1) purifying obtains 4-(2-hydroxy phenyl) methyl crotonate (1.8g, productive rate: 91%) be weak yellow liquid.
m/z：[M+H] +193.1
Step 3:2-(2,3-Dihydrobenzofuranes-2-base) methyl acetate
4-(2-hydroxy phenyl) methyl crotonate (1.8g that step 2 is prepared, 9.36mmol) with Xin Keni (0.83g, chloroform (60mL) mixing solutions 2.81mmol) reacts four days at 35 DEG C, vacuum rotary steam is except desolventizing, obtain residue over silica gel column chromatography (sherwood oil: ethyl acetate=20: 1 ~ 10: 1) purifying obtains 2-(2,3-Dihydrobenzofuranes-2-base) methyl acetate (1.5g, productive rate 83%) is colourless liquid.
1H NMR(400MHz，CDCl 3)：δ7.12-7.20(m，2H)，6.80-6.89(m，2H)，5.16-5.24(m，1H)3.76(s，3H)，3.44(dd，J＝9.2，15.6Hz，1H)，2.88-2.99(m，2H)，2.71(dd，J＝6.4，16.0Hz，1H)。
Step: 4:2-(2,3-Dihydrobenzofuranes-2-base) ethanol
Under condition of ice bath, to the 2-(2 that step 3 prepares, 3-Dihydrobenzofuranes-2-base) methyl acetate (1.5g, add Lithium Aluminium Hydride (0.37g in tetrahydrofuran (THF) (15mL) solution 7.80mmol) in batches, 9.75mmol), stir 30 minutes under reaction system ice bath, add sal glauberi cancellation reaction, reaction system diatomite filtration, filtrate decompression is revolved and is steamed except desolventizing obtains 2-(2,3-Dihydrobenzofuranes-2-base) ethanol (1.15g, productive rate: 90%) be colourless liquid.
m/z：[M+H] +165.1
Step 5:2-(2-bromotrifluoromethane)-2,3-Dihydrobenzofuranes
The 2-(2 that step 4 is prepared, 3-Dihydrobenzofuranes-2-base) ethanol (0.35g, 2.13mmol) with triphenylphosphine oxide (0.84g, 3.2mmol) be dissolved in methylene dichloride (10mL), under condition of ice bath, add carbon tetrabromide (1.1g in reaction solution in batches, 3.2mmol), reaction system stirred at ambient temperature 1 hour, add saturated sodium bicarbonate aqueous solution (10mL) cancellation reaction, extract with methylene dichloride (30mL × 2), merge organic phase, with anhydrous sodium sulfate drying, vacuum rotary steam is except desolventizing, obtain resistates thin layer and prepare plate (sherwood oil: ethyl acetate=10: 1) purifying obtains 2-(2-bromotrifluoromethane)-2, 3-Dihydrobenzofuranes (450mg, productive rate 93%) be pale yellow oil.
Embodiment 89:(S)-2 (brooethyl)-2,3-synthesis of Dihydro-benzofuran and (S)-(2,3-Dihydrobenzofuranes-2-base) methyl alcohol
Step 1:(R)-1-(benzyl oxygen base)-3-(2-p-methoxy-phenyl) propan-2-ol
At-78 DEG C, be added dropwise to n-Butyl Lithium (25.7mL, 64.2mmol, 2.5M hexane solution), finish in the anhydrous tetrahydrofuran solution of 2-bromoanisole (12g, 64.2mmol), reaction solution stirs 1 hour in-78 DEG C.By cuprous bromide dimethyl sulphide (6.6g, 32.1mmol) disposablely add in above-mentioned solution, temperature of reaction is slowly warming up to-40 DEG C from-78 DEG C, and then be cooled to-60 DEG C, drip (R)-2-benzyloxymethyl oxyethane (2.63g, 16.1mmol), boron trifluoride diethyl etherate (109mg, 0.77mmol) is added subsequently.Mixture rises to stirred overnight at room temperature.Add sherwood oil, insolubles is crossed and is filtered, filter cake ethyl acetate is washed, filtrate concentrates and uses silica gel chromatography column purification (sherwood oil: ethyl acetate=10: 1 to 5: 1) obtain (R)-1-(benzyl oxygen base)-3-(2-p-methoxy-phenyl) propan-2-ol (2.9g, productive rate 66%), be colorless oil.
1H NMR(400MHz，CDCl 3)：δ7.30-7.40(m，5H)，7.17-7.27(m，2H)，6.88-6.94(m，2H)，4.58(s，2H)，4.11-4.16(m，1H)，3.84(s，3H)，3.54(dd，J＝4，9.6Hz，1H)，3.44(dd，J＝6.8，10Hz，1H)，2.83-2.92(m，2H)，2.58(br，1H)。
Step 2:(R) the bromo-3-of-1-(2-hydroxy phenyl) third-2-base ethyl ester
By (R)-1-(benzyl oxygen base)-3-(2-p-methoxy-phenyl) propan-2-ol (2.9g, 10.7mmol) be dissolved in the acetic acid solution (25mL) of 33% hydrogen bromide, 70 DEG C of heated and stirred are spent the night, solvent removed in vacuo, residue over silica gel chromatography (sherwood oil: ethyl acetate=5: 1) obtain the bromo-3-of (R)-1-(2-hydroxy phenyl) third-2-base ethyl ester (2.2g, productive rate 75%), be pale yellow oil.
1H NMR(400MHz，CDCl 3)：δ7.12-7.20(m，2H)，6.86-6.89(m，2H)，5.09-5.12(m，1H)，3.59(dd，J＝3.6，7.2Hz，1H)，3.48(dd，J＝5.6，10.8Hz，1H)，3.08(dd，J＝5.2，14.0Hz，1H)，2.98(dd，J＝6.0，14.4Hz，1H)，2.15(s，3H)。
Step 3:(R)-2-(the bromo-hydroxypropyl of 3-) phenol
Under room temperature, to the bromo-3-of (R)-1-(2-hydroxy phenyl) third-2-base ethyl ester (2.2g, diethyl ether solution (the 10mL of hydrogenchloride is added in methyl alcohol (20mL) solution 8.05mmol), 20mmol, 2.0M), mixture stirred overnight at room temperature.Solvent removed in vacuo, residue over silica gel chromatography (sherwood oil: ethyl acetate=5: 1) obtaining (R)-2-(the bromo-hydroxypropyl of 3-) phenol (1.7g, productive rate 91%), is light-red oil.
1H NMR(400MHz，CDCl 3)：δ7.56(s，1H)，7.18-7.22(m，1H)，7.07-7.09(m，1H)，6.93-6.95(m，1H)，6.86-6.90(m，1H)，4.22(br，1H)，3.54(dd，J＝3.6，10.4Hz，1H)，3.38(dd，J＝8.4，10.4Hz，1H)，3.23(br s，1H)，3.05(dd，J＝3.2，14.4Hz，1H)，2.94(dd，J＝7.2，14.4Hz，1H)。
Step 4:(S)-2-(brooethyl)-2,3-Dihydrobenzofuranes
Under room temperature, to (R)-2-(the bromo-hydroxypropyl of 3-) phenol (1.7g, triphenylphosphine (4.82g is added in tetrahydrofuran (THF) (20mL) solution 7.36mmol), 18.4mmol), be added dropwise to DEAD (3.2g subsequently, 18.4mmol), mixture stirred overnight at room temperature, directly obtain (S)-2-(brooethyl)-2 with silica gel chromatography column purification (sherwood oil), 3-Dihydrobenzofuranes (1.1g, productive rate 70%), be yellow oil.
1H NMR(400MHz，CDCl 3)：δ7.13-7.21(m，2H)，6.83-6.90(m，1H)，6.82(d，J＝8.0Hz，1H)，4.99-5.06(m，1H)，3.63(dd，J＝5.2，10.4Hz，1H)，3.53(dd，J＝6.8，10.4Hz，1H)，3.42(dd，J＝9.2，16.0Hz，1H)，3.15(dd，J＝6.4，15.6Hz，1H)。
[α] D 25＝+19.3°(C＝0.558％，MeOH)
Step 5:(S)-(2,3-Dihydrobenzofuranes-2-base) methyl acetate
Get (S)-2-(brooethyl)-2,3-Dihydrobenzofuranes (200mg, 0.94mmol) be placed in round-bottomed flask, add DMF (2mL), add potassium acetate (921mg, 9.39mmol), heat 75 DEG C of stirrings to spend the night, add diluted ethyl acetate, wash (x 3) and saturated common salt water washing with water, anhydrous sodium sulfate drying, version purifying (sherwood oil: ethyl acetate=5: 1) obtain (S)-(2 prepared by concentrated silica gel, 3-Dihydrobenzofuranes-2-base) methyl acetate (108mg, productive rate 54%) is pale yellow oil.
1H NMR(400MHz，CDCl 3)：δ7.13-7.20(m，2H)，6.83-6.90(m，2H)，4.99-5.06(m，1H)，4.36(dd，J＝3.6，12Hz，1H)，4.23(dd，J＝7.2，12Hz，1H)，3.34(dd，J＝10，16Hz，1H)，3.00(dd，J＝7.6，16Hz，1H)，2.12(s，3H)。
Step 6:(S)-(2,3-Dihydrobenzofuranes-2-base) methyl alcohol
Get (S)-(2, 3-Dihydrobenzofuranes-2-base) methyl acetate (100mg, 0.52mmol) be placed in round-bottomed flask, add methyl alcohol (2mL) and 2 to drip, add salt of wormwood (54mg, 0.39mmol), stirring at room temperature 30 minutes, add ethyl acetate (10mL), with water and saturated common salt water washing, anhydrous sodium sulfate drying, filtering and concentrating and with silica gel prepare plate purify (sherwood oil: ethyl acetate=3: 1) (S)-(2, 3-Dihydrobenzofuranes-2-base) methyl alcohol (60mg, productive rate 60%, e.e. be worth: 97.3%), for pale yellow oil.
1H NMR(400MHz，CDCl 3)：δ7.12-7.20(m，2H)，6.80-6.89(m，2H)，4.90-4.97(m，1H)，3.86-3.89(m，1H)，3.75-3.78(m，1H)，3.27(dd，J＝9.6，15.6Hz，1H)，3.04(dd，J＝7.2，15.6Hz，1H)，1.99(br，1H)。
Embodiment 90:(R)-2 (brooethyl)-2,3-synthesis of Dihydro-benzofuran
By the synthetic method of embodiment 89 compound, be chiral raw material preparation (R)-2 (brooethyl)-2,3-Dihydro-benzofuran with (S)-2-benzyloxymethyl oxyethane.
1H NMR(400MHz，CDCl 3)：δ7.13-7.21(m，2H)，6.90(m，1H)，6.82(d，J＝8.0Hz，1H)，4.99-5.06(m，1H)，3.63(dd，J＝5.2，10.4Hz，1H)，3.53(dd，J＝6.8，10.4Hz，1H)，3.42(dd，J＝9.2，16.0Hz，1H)，3.15(dd，J＝6.4，15.6Hz，1H)。
[α] D 25＝-33.4°(C＝0.515％，MeOH)
Embodiment 91:(2S, 3R, 4R, 5S, 6R) the synthesis (Compound I-1) of-2-(3-(4-(cumarone-2-ylmethoxy) benzyl)-4-chloro-phenyl-)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol
Step 1:(2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl) 6-(3-(4-(cumarone-2 ylmethoxy) benzyl)-4-chloro-phenyl-) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester
By (the 2R that embodiment 71 prepares, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl)-6-(the chloro-3-of 4-(4-hydroxybenzyl) phenyl) tetrahydrochysene-2H-pyrans-3, 4, 5-tri-base triacetyl ester (150mg, 0.27mmol), 2-(chloromethyl) cumarone (91mg that embodiment 87 prepares, 0.55mmol) with cesium carbonate (107mg, N 0.33mmol), dinethylformamide (2mL) mixed solution is heated to 50 DEG C and stirs 2 hours, dilute by ethyl acetate (50mL), organic phase washed with water and saturated common salt water washing, use anhydrous sodium sulfate drying.Vacuum rotary steam is except desolventizing, crude product thin layer prepares plate (petrol ether/ethyl acetate=2: 1) purifying obtains (2R, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl) 6-(3-(4-(cumarone-2 ylmethoxy) benzyl)-4-chloro-phenyl-) tetrahydrochysene-2H-pyrans-3,4,5-tri-base triacetyl ester (75mg, productive rate: 40%) be white solid.
Step 2:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2-ylmethoxy) benzyl)-4-chloro-phenyl-)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol
To (the 2R that step 1 prepares, 3R, 4R, 5S, 6S)-2-(acetoxy-methyl) 6-(3-(4-(cumarone-2 ylmethoxy) benzyl)-4-chloro-phenyl-) tetrahydrochysene-2H-pyrans-3, 4, 5-tri-base triacetyl ester (75mg, sodium methylate (9mg is added in methyl alcohol (3mL) 0.11mmol) and tetrahydrofuran (THF) (1mL) mixing solutions, 0.17mmol), reaction solution stirred at ambient temperature neutralized with acetic acid after 2 hours, underpressure distillation desolventizes, crude product directly with thin layer prepare plate purify (developping agent: methylene chloride/methanol=10: 1) (2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2 ylmethoxy) benzyl)-4-chloro-phenyl-)-6-(methylol) tetrahydrochysene-2H-pyrans-3, 4, 5-triol (48mg, productive rate: 85%) be white solid.
m/z：[M+Na] +533.0
Utilize the synthetic method of embodiment 91 compound, and bromo-derivative or chloro thing carry out synthetic example 92 ~ 103 compound accordingly
Embodiment 92:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2-ylmethoxy) benzyl)-4-aminomethyl phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-3)
m/z：[M+NH 4] +507.9
Embodiment 93:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2-ylmethoxy) benzyl)-4-fluorophenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-2)
m/z：[M+NH 4] +511.9
Embodiment 94:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2-ylmethoxy)-3-luorobenzyl)-4-chloro-phenyl-)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-11)
m/z：[M+H 2O] +546.2
Embodiment 95:(2S, 3R, 4R, 5S, 6R)-2-(3-(4-(cumarone-2-ylmethoxy) benzyl)-phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-6)
m/z：[M+NH 4] +494.0
Embodiment 96:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((7-Fluorobenzofur-2-base) methoxyl group) benzyl)-phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-10)
m/z：[M+NH 4] +545.8
1H NMR(400MHz，CDCl 3)：δ7.35-7.41(m，3H)，7.29-7.31(m，1H)，7.16-7.23(m，3H)，7.07-7.10(m，1H)，6.95-6.99(m，3H)，5.20(s，2H)，4.02-4.12(m，3H)，3.88-3.91(m，1H)，3.68-3.73(m，1H)，3.28-3.49(m，4H)。
Embodiment 97:2-(4-(cumarone-2-ylmethoxy) benzyl)-4-((2S, 3R, 4R, 5S, 6R)-3,4,5-trihydroxy--6-(methylol) tetrahydrochysene-2H-pyrans-2-bases) cyanophenyl (Compound I-4)
m/z：[M+NH 4] +518.9
Embodiment 98:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((6-Fluorobenzofur-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-9)
m/z：[M+NH 4] +545.9
1H NMR(400MHz，CDCl 3)：δ7.57(dd，J＝5.6，8.4Hz，1H)，7.35-7.38(m，2H)，7.28-7.31(m，2H)，7.14-7.17(m，2H)，7.02-7.07(m，1H)，6.94-6.97(m，2H)，6.87(s，1H)，5.15(s，2H)，4.01-4.12(m，3H)，3.88-3.91(m，1H)，3.69-3.73(m，1H)，3.28-3.49(m，4H)。
Embodiment 99:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((5-Fluorobenzofur-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-8)
m/z：[M+NH 4] +545.9
1H NMR(400MHz，CDCl 3)：δ7.47(dd，J＝4.0，8.8Hz，1H)，7.35-7.37(m，2H)，7.27-7.31(m，2H)，7.14-7.16(m，2H)，7.03-7.08(m，1H)，6.93-6.97(m，2H)，6.86(s，1H)，5.15(s，2H)，4.01-4.12(m，3H)，3.88-3.91(m，1H)，3.69-3.73(m，1H)，3.29-3.50(m，4H)。
Embodiment 100:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-((4-Fluorobenzofur-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-7)
m/z：[M+NH 4] +545.9
1H NMR(400MHz，CDCl 3)：δ7.27-7.38(m，5H)，7.15-7.17(m，2H)，6.95-6.99(m，4H)，5.17(s，2H)，4.01-4.12(m，3H)，3.87-3.91(m，1H)，3.69-3.73(m，1H)，3.29-3.50(m，4H)。
Embodiment 101:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(2-(2,3-Dihydrobenzofuranes-2-base) oxyethyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-60)
m/z：[M+NH 4] +543.9
Embodiment 102:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(((S)-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-23)
I-23 retention time RT=4.09min (e.e. value: 100%) is recorded by chiral analysis method A.
m/z：[M+Na] +535.2
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.20(dd，J＝0.4，7.6Hz，1H)，7.07-7.14(m，3H)，6.82-6.88(m，3H)，6.74(d，J＝8.0Hz，1H)，5.06-5.13(m，1H)，4.00-4.14(m，5H)，3.87-3.90(m，1H)，3.68-3.73(m，1H)，3.28-3.49(m，5H)，3.14(dd，J＝7.2，16Hz，1H)。
[α] D 25＝+50.0°(C＝0.054％，MeOH)
Compound I-23 can be synthesized according to the synthetic method of method two embodiment 77 compound.
Embodiment 103:(2S, 3R, 4R, 5S, 6R)-2-(the chloro-3-of 4-(4-(((R)-2,3-Dihydrobenzofuranes-2-base) methoxyl group) benzyl) phenyl)-6-(methylol) tetrahydrochysene-2H-pyrans-3,4,5-triol (Compound I-24)
I-24 retention time RT=4.92min (e.e. value: 98.1%) is recorded by chiral analysis method A.
m/z：[M+Na] +535.2
1H NMR(400MHz，CD 3OD)：δ7.28-7.37(m，3H)，7.20(dd，J＝0.4，7.6Hz，1H)，7.07-7.13(m，3H)，6.82-6.87(m，3H)，6.74(d，J＝8.0Hz，1H)，5.07-5.11(m，1H)，4.00-4.13(m，5H)，3.87-3.90(m，1H)，3.68-3.73(m，1H)，3.28-3.49(m，5H)，3.14(dd，J＝7.2，16Hz，1H)。
[α] D 25＝-21.8°(C＝0.055％，MeOH)
Compound I-24 can be synthesized according to the synthetic method of method two embodiment 77 compound.
Bioassay embodiment:
Effect example 1:[ 14c] absorption test (SGLT-1 and SGLT-2 Activity Screening Test) of-AMG in the Flp-in Chinese hamster ovary celI of stably express people sodium ion dependent glucose absorption ionic channel I and II
Clone from the cDNA of OriGene purchaser SGLT-1 and SGLT-2.After obtaining sequence information, be building up on pcDNA5/FRT carrier with traditional molecular biology method, then use the method for Lipofetamin2000 liposome transfection that expression plasmid is imported Flp-in Chinese hamster ovary celI.Cell after transfection carries out hygromycin resistance screening, by the method screening single cell clone of gradient dilution after 4-5 week.Use the method for RT-PCR and functional trial to detect the expression of SGLT-1 and SGLT-2 afterwards, select high-selenium corn signal cell strain to be used for follow-up test.
Cultivate the Flp-in Chinese hamster ovary celI strain of stably express SGLT-1 and SGLT-2, substratum consists of: F12 substratum (purchase of Invitrogen company), 500 μ g/ml Totomycin (purchase of Calbiochem company) and 10% foetal calf serum (purchase of Invitrogen company).When cell grows to 80% fusion, phosphoric acid buffer (purchase of the Invitrogen company) cleaning three times of cell in T75 culturing bottle, add the pancreas enzyme-EDTA solution (purchase of Invitrogen company) of 5mL afterwards, rock cultivation bottle gently, make all cells contact Digestive system, take off until cell and to add substratum 10mL transfer pipet after wall cell is blown out single cell suspension.Adjustment cell density is 3 × 105/mL, is then inoculated in the saturating Tissue Culture Plate (purchase of Coming company) of the wall white background in 96 holes by the amount in 100 μ L/ holes.
Absorption test can be carried out after cell pellet overnight cultivation in orifice plate is adherent.In cell inoculation after at least 12 hours, with absorbent solution KRH-NMG (the 120mM N-methyl-D-glucarnine (NMG) in 150 μ L/ holes, 4.7mM Repone K, 1.2mM magnesium chloride, 2.2mM calcium chloride, 10mM hydroxyethyl piperazine second thiosulfonic acid (HEPES), pH 7.4 comprises 1mM Tutofusin tris (Tris)) wash cell once.Blot each hole, containing 2.5 μ Ci/mL's 14buffer KRH-Na+ (the 120mM sodium-chlor of methyl-α-D-glucopyranoside solution (AMG) (purchase of PerkinElmer company) of C mark, 4.7mM Repone K, 1.2mM magnesium chloride, 2.2mM calcium chloride, 10mM hydroxyethyl piperazine second thiosulfonic acid (HEPES), pH 7.4 comprises 1mM Tutofusin tris (Tris)) be added in the cleaned hole of Buffer KRH-Na+ and KRH-NMG with 45 μ L/ holes.Add corresponding Compound I immediately afterwards, 5 μ L/ holes, ensure that the concentration of dimethyl sulfoxide (DMSO) is 1% (volumetric concentration).The plank adding compound is cultivated one hour in 37 DEG C of incubators.After time terminates, add in each hole immediately 150 μ L ice-cold wash plate liquid (120mM sodium-chlor, 4.7mM Repone K, 1.2mM magnesium chloride, 2.2mM calcium chloride, 10mM hydroxyethyl piperazine second thiosulfonic acid (HEPES), 0.5mM phlorizin (phlorizin), pH 7.4with 1mM Tutofusin tris (Tris)), to stop absorption test.Cleaning every Kong Sanci with washing plate liquid, finally blotting liquid in orifice plate as far as possible.In cleaning process, avoid cell detachment as far as possible.The Lysis Buffer (0.1mM sodium hydroxide) in 20 μ L/ holes join institute porose in, Sptting plate is placed on vibrator with the velocity fluctuation 5 minutes of 900rpm.The scintillation solution Microsint40 in 80 μ L/ holes join institute porose in, with the velocity fluctuation 5 minutes of 900rpm.Finally, microwell plate is delivered on MicroBeta Trilux (production of PerkinElmer company) instrument and is measured radioactivity.Analytical data, with each Compound I C of XL-fit computed in software 50.The results are shown in Table 1, table 1 shows selects compound to the IC of SGLT-1 and SGLT-2 50numerical value.
Table 1:
Wherein, A:Empagliflozin (Chinese: Li Gelie is clean, No. CAS: 864070-44-0); B:Canagliflozin (Chinese: Kan Gelie is clean, No. CAS: 842133-18-0) is two kinds of known aryl glycoside SGLT-2 inhibitor.Its structure is as follows:
Effect example 2: glucose in urine discharges test (Urinary Glucose Excretion in C57MiceTest)
Growing up, (C57 mouse sex is male to C57 mouse; Supplier: Shanghai Slac Experimental Animal Co., Ltd.) (body weight is greater than 20g) give to give glucose (5g/Kg) through gi tract again after test-compound I (10mg/Kg) through gi tract, tested C57 mouse is put into metabolic cage, collect discharge urine volume total in 24 hours, the normal saline flushing of metabolic cage 5mL, during collection is urinated, (24 hours) mouse can freely be fetched water and food.Collect after urine sample, frozen immediately, detect glucose content in urine sample subsequently, last total glucose in urine output according to total urine volume computing animal, then change the glucose in urine output (mg) in 200mg body weight 24 hours into according to mice weights.Result (mean values of 6 mouse) is in table 2.
Table 2:
Compound number Glucose in urine output (mg/200mg body weight/24h)
A 1536.5
I-1 1646.9
I-22 3106.6
I-36 1723.1
Wherein, A:Empagliflozin (Chinese: Li Gelie is clean, No. CAS: 864070-44-0) is known aryl glycoside SGLT-2 inhibitor, and concrete structure is listed in effect example 1.
As can be seen from Table 2, the experiment made on the living effect of Compound I-1 of the present invention and Compound I-22 obviously will be better than the effect of compd A of the prior art (Li Gelie is clean).
In sum, the effect of compound of the present invention in experiment made on the living has outstanding progress relative to prior art.

Claims (21)

1. one kind such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I;
Wherein, X is O or S; L is CH 2, CD 2, C=O or CF 2; Z is CH 2, S, O or singly-bound; W is singly-bound or (CH 2) n, n=1,2 or 3;
Y is bicyclic radicals; Described bicyclic radicals is substituted or is not substituted; Described bicyclic radicals is C 5-8cycloalkyl C 6aryl, C 5-8cycloalkyl C 3-5heteroaryl, C 3-7heterocyclylalkyl C 6aryl, C 3-7heterocyclylalkyl C 3-5heteroaryl, C 6aryl C 6aryl, C 3-5heteroaryl C 6aryl or C 3-5heteroaryl C 3-5heteroaryl;
When described bicyclic radicals is substituted, described bicyclic radicals is substituted in optional position by following one or more group: alkyl, CN, halogen, CF 3, OH, amino, C 2-6alkynyl, C 2-6thiazolinyl, alkoxyl group, halogenated alkoxy, cycloalkyl, cycloalkyl oxy, cycloalkylalkyl, cycloalkyl alkoxy, Heterocyclylalkyl, Heterocyclylalkyl oxygen base, hetercycloalkylalkyl, heterocycloalkylalkoxy, alkylamino, carbonyl, COOH, COOR 7, COR 7, CONR 7r 7a,-NHCOR 7,-NHSO 2r 7, aryl, heteroaryl, alkyl sulphonyl, aryl sulfonyl, or heteroarylsulfonyl;
In the substituting group of bicyclic radicals, described substituting group for not replace, or further replace by following one or more group: alkyl, halogen, CF 3, OH, CN, amino, alkoxyl group or halogenated alkoxy;
R 1for H, halogen, CN, alkyl, alkoxyl group, halogenated alkoxy, OCD 3, OC 2d 5or CF 3;
R 2for H, alkyl, halogen, CF 3, CN, OH, amino, alkoxyl group, halogenated alkoxy, OCD 3, OC 2d 5, C 2-6alkynyl, C 2-6thiazolinyl, cycloalkyl, Heterocyclylalkyl, alkylamino, carbonyl, COOH, COOR 7, COR 7, CONR 7r 7a,-NHCOR 7,-NHSO 2r 7, aryl, heteroaryl, alkyl sulphonyl, aryl sulfonyl or heteroarylsulfonyl;
R 7and R 7abe selected from alkyl, cycloalkyl or Heterocyclylalkyl independently of one another, or, R 7and R 7athe Heterocyclylalkyl of 3-7 unit is formed together with the atom N that they connect.
2. as claimed in claim 1 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: at R 7and R 7aformed together with the atom N that they connect in the Heterocyclylalkyl of 3-7 unit, the Heterocyclylalkyl of described 3-7 unit is also further containing 1-3 following atom or group: N, O, S, SO and SO 2;
At R 7and R 7aformed together with the atom N that they connect in the Heterocyclylalkyl of 3-7 unit, the Heterocyclylalkyl of described 3-7 unit is replaced by alkyl and/or methylsulfonyl.
3. as claimed in claim 1 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: in described Y, described C 3-7heterocyclylalkyl C 6aryl is C 4 ~ 6heterocyclylalkyl C 6aryl; Described C 3-5heteroaryl C 6aryl is C 3-4heteroaryl C 6aryl.
4. as claimed in claim 1 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: described Y is any one in following Y1 to Y24:
Wherein, R 3, R 4, R 5, R 6, R 6a, R 6band R 6cbe H, alkyl, CN, halogen, CF independently of one another 3, OH, amino, C 2-6alkynyl, C 2-6thiazolinyl, alkoxyl group, halogenated alkoxy, cycloalkyl, cycloalkyl oxy, cycloalkylalkyl, cycloalkyl alkoxy, Heterocyclylalkyl, Heterocyclylalkyl oxygen base, hetercycloalkylalkyl, heterocycloalkylalkoxy, alkylamino, carbonyl, COOH, COOR 7, COR 7, CONR 7r 7a,-NHCOR 7,-NHSO 2r 7, aryl, heteroaryl, alkyl sulphonyl, aryl sulfonyl or heteroarylsulfonyl;
Or, described R 6aand R 6bthe cycloalkyl of 3-8 unit is formed together with the atom that they connect, or 3-8 unit Heterocyclylalkyl; The cycloalkyl of described 3-8 unit or 3-8 unit Heterocyclylalkyl for not replace, or by alkyl and/or halogen substiuted.
5. as claimed in claim 4 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: work as R 6aand R 6bwhen the atom be connected with them does not form cycloalkyl or Heterocyclylalkyl, described R 3, R 4, R 5, R 6, R 6a, R 6band R 6creplaced by following any group further: alkyl, halogen, CF 3, OH, CN, amino, alkoxyl group or halogenated alkoxy;
Work as R 6aand R 6bform the cycloalkyl of 3-8 unit together with the atom that they connect, or during 3-8 unit Heterocyclylalkyl, described 3-8 unit Heterocyclylalkyl contains 1-3 following atom or group: N, O, S, SO and SO 2.
6. as claimed in claim 1 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: described Y is
* the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
7. as described in any one of Claims 1 to 5 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: described is IA, IB or IC such as formula the C-aryl glycoside derivative shown in I
In IA, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2;
In IB, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type;
In IC, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
8. as claimed in claim 7 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that: such as formula the C-aryl glycoside derivative shown in IA be:
Wherein, X is O; L is CH 2; Z is O; W is (CH 2) n, n=1 or 2; R 1for H or F; R 2for H, F, Cl, CN, CH 3or OCH 3; R 3, R 4, R 5be separately H, methyl or F; R 6for H;
Such as formula the C-aryl glycoside derivative shown in IB be:
Wherein, X is O; L is CH 2; Z is O; W is (CH 2) n, n=1 or 2; R 1for H or F; R 2for H, F, Cl, CN, CH 3or OCH 3; R 3, R 4, R 5be separately H, methyl or F; R 6a, R 6b, R 6cbe separately H or methyl; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type;
Such as formula the C-aryl glycoside derivative shown in IC be:
In IC, X is O; L is CH 2; Z is O; W is (CH 2) n, n is 1 or 2; R 1for H or F; R 2for H, F, Cl, CN, CH 3or OCH 3; R 3, R 4, R 5be separately H, methyl or F; R 6a, R 6b, R 6cbe separately H or CH 3; * the carbon marked is chiral carbon, and it comprises racemic modification, S type or R type.
9. as claimed in claim 1 such as formula C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or the pharmacy acceptable salt shown in I, it is characterized in that:
Described is the arbitrary compound of following I-1 ~ I-148 such as formula the C-aryl glycoside derivative shown in I:
10. the preparation method of C-aryl glycoside derivative, its steric isomer, isotropic substance substitutive derivative, prodrug or pharmacy acceptable salt as described in any one of claim 1 ~ 9, it is following either method:
Method one: described preparation method comprises the steps: in solvent, under the effect of alkali, carries out the reaction of deacetylation protecting group by compound 1-f;
Method two: described preparation method comprises the steps: 1) in solvent, under the effect of condensing agent, by compound 2-g with carry out Mitsunobu reaction; 2) in solvent, under the effect of alkali, by step 1) the compound 2-f that obtains carries out the reaction of deacetylation protecting group;
Method three: described preparation method comprises the steps: 1, in solvent, under the effect of alkali, by compound 3-g with mixing, carries out nucleophilic substitution reaction; 2, in solvent, under the effect of alkali, compound 3-f step 1 obtained carries out the reaction of deacetylation protecting group;
Wherein, V is chlorine, bromine or iodine.
11. preparation methods as claimed in claim 10, is characterized in that: in method one, and described deacetylation protecting group reaction adopts sodium methylate and methanol system, or lithium hydroxide, methyl alcohol, tetrahydrofuran (THF) and aqueous systems; Described solvent is the mixed solvent of methyl alcohol or methyl alcohol, tetrahydrofuran (THF) and water; When adopting mixed solvent, the volume ratio of methyl alcohol, tetrahydrofuran (THF) and water is 4: 1: 0.5 ~ 0.5: 1: 0.5; Described alkali is sodium methylate or lithium hydroxide; The mol ratio of described alkali and compound 1-f is 0.1: 1 ~ 3: 1; The temperature of described reaction is 10 ~ 30 DEG C.
12. preparation methods as claimed in claim 10, it is characterized in that: in method one, described deacetylation protecting group reaction comprises the steps: in solvent, and compound 1-e is carried out ethanoyl protective reaction, carry out recrystallization again, obtain described compound 1-f;
13. preparation methods as claimed in claim 12, it is characterized in that: in method one, described deacetylation protecting group reaction comprises the steps: in solvent, is mixed by compound 1-d with triethyl silicane and boron trifluoride diethyl etherate system, carry out reduction reaction, obtain described compound 1-e;
14. preparation methods as claimed in claim 13, is characterized in that: in method one, and described deacetylation protecting group reaction comprises the steps: in solvent, at-78 DEG C ~-60 DEG C, by compound 1-c and organolithium reagent hybrid reaction 0.5 ~ 1 hour, then with 2,3,4,6-tetra--O-(TMS)-D-glucopyra saccharon mixes, and at 10 ~ 30 DEG C, then mixes with the methanol solution of methylsulfonic acid, react, obtain described compound 1-d;
Wherein, Q is bromine or iodine.
The compound of 15. following arbitrary structures;
Wherein, Q is bromine or iodine, R 1, R 2, W and Y be with according to any one of claim 1 ~ 9.
16. 1 kinds of pharmaceutical compositions, it comprise as described in any one of claim 1 ~ 9 such as formula C-aryl glycoside derivative, its pharmacy acceptable salt and/or the prodrug shown in I, and pharmaceutically acceptable auxiliary material.
17. pharmaceutical compositions as claimed in claim 16, is characterized in that: described pharmaceutical composition contains the medicine for the treatment of diabetes or the therapeutical agent of other kind of other kind further; Described pharmaceutically acceptable auxiliary material is pharmaceutically acceptable carrier, thinner and/or vehicle.
18. pharmaceutical compositions as claimed in claim 17, is characterized in that: the medicine of the treatment diabetes of other described kind or the therapeutical agent of other kind are one or more in Remedies for diabetes, treating diabetic complications agent, lipid-lowering therapy agent, obesity treatment agent and hypertension therapeutic agent.
19. as described in any one of claim 1 ~ 9 such as formula C-aryl glycoside derivative, its pharmacy acceptable salt or the prodrug shown in I, or pharmaceutical composition as described in any one of claim 16 ~ 18 application in preparation SGLT inhibitor.
20. as described in any one of claim 1 ~ 9 such as formula C-aryl glycoside derivative, its pharmacy acceptable salt or the prodrug shown in I, or pharmaceutical composition as described in any one of claim 16 ~ 18 application in the medicine of the relative disease of preparation treatment SGLT mediation.
21. apply as claimed in claim 20, it is characterized in that: the relative disease of described SGLT mediation comprises: type ii diabetes, type i diabetes, diabetic complication, obesity, high blood pressure disease, and the metabolic trouble relevant to diabetes.
CN201510151483.XA 2014-04-14 2015-04-01 C-aryl glucoside derivative, as well as medical composition, preparation method and application thereof Pending CN105017236A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106928040A (en) * 2015-12-31 2017-07-07 上海医药工业研究院 The preparation method of SGLT2 inhibitor intermediate
US9914724B2 (en) 2014-04-14 2018-03-13 Shanghai De Novo Pharmatech Co., Ltd. C-aryl glycosid derivatives, pharmaceutical composition, preparation process and uses thereof
CN109988204A (en) * 2018-01-03 2019-07-09 上海迪诺医药科技有限公司 O- aryl glycoside derivative, its pharmaceutical composition and application
CN112047915A (en) * 2019-06-05 2020-12-08 吉林惠升生物制药有限公司 Novel preparation process of C-glycoside derivatives

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CN102875503A (en) * 2011-06-25 2013-01-16 山东轩竹医药科技有限公司 C-glucoside derivative
CN105001213A (en) * 2014-04-14 2015-10-28 上海迪诺医药科技有限公司 C-aryl glycoside derivative, pharmaceutical composition, preparation method and application thereof

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CN102643256A (en) * 2011-02-18 2012-08-22 上海璎黎科技有限公司 Arylglucoside compound and preparation method and application thereof
CN102875503A (en) * 2011-06-25 2013-01-16 山东轩竹医药科技有限公司 C-glucoside derivative
CN105001213A (en) * 2014-04-14 2015-10-28 上海迪诺医药科技有限公司 C-aryl glycoside derivative, pharmaceutical composition, preparation method and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9914724B2 (en) 2014-04-14 2018-03-13 Shanghai De Novo Pharmatech Co., Ltd. C-aryl glycosid derivatives, pharmaceutical composition, preparation process and uses thereof
CN106928040A (en) * 2015-12-31 2017-07-07 上海医药工业研究院 The preparation method of SGLT2 inhibitor intermediate
CN109988204A (en) * 2018-01-03 2019-07-09 上海迪诺医药科技有限公司 O- aryl glycoside derivative, its pharmaceutical composition and application
CN109988204B (en) * 2018-01-03 2023-01-24 江西青峰药业有限公司 O-aryl glycoside derivative, and pharmaceutical composition and application thereof
CN112047915A (en) * 2019-06-05 2020-12-08 吉林惠升生物制药有限公司 Novel preparation process of C-glycoside derivatives
CN112047915B (en) * 2019-06-05 2023-02-17 北京惠之衡生物科技有限公司 Novel preparation process of C-glycoside derivatives

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