CN105012286A - Use of natural fatty acid disinfectant in preparation of foot and mouth disease virus killing preparation - Google Patents
Use of natural fatty acid disinfectant in preparation of foot and mouth disease virus killing preparation Download PDFInfo
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- CN105012286A CN105012286A CN201510357032.1A CN201510357032A CN105012286A CN 105012286 A CN105012286 A CN 105012286A CN 201510357032 A CN201510357032 A CN 201510357032A CN 105012286 A CN105012286 A CN 105012286A
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Abstract
The invention discloses a use of a natural fatty acid disinfectant in preparation of a foot and mouth disease virus killing preparation. The natural fatty acid disinfectant has total fatty acid content of 9.9-11.9wt%. The natural fatty acid disinfectant is prepared from 4.6-5.5wt% of nonanoic acid, 3.1-3.7wt% of octanoic acid, 2.1-2.7wt% of capric acid and a certain amount of a solvent. The natural fatty acid disinfectant has a foot and mouth disease virus killing ratio of 100%.
Description
Technical field
The present invention relates to disinfectant applied technical field, particularly a kind of natural acid disinfectant is preparing the application killed in hoof-and-mouth disease toxin preparation.
Background technology
Foot and mouth disease Aftosa (belonging to a class infectious disease) popular name " aphtha ", " warding off Huang " are that the one artiodactylous caused by foot and mouth disease virus is acute, hot, high degree in contact sexually transmitted disease.Main infringement artiodactyl beast, occasionally in people and other animals.It faces to examine and is characterized as oral mucosa, hoof and skin of breast generation vesicle.
The one that the Some Livestocks such as foot and mouth disease is pig, cattle, sheep and other domestic, wild artiodactyl suffer from altogether is acute, hot, high degree in contact sexually transmitted disease, and susceptible animal reaches kind more than 70.Clinical symptoms is in oral mucosa, hoof and skin of breast generation vesicle rash.This sick route of transmission is many, speed is fast, once repeatedly worldwide outbreak of epidemic, causes huge politics, economic loss.Given this, OIE (OIE) is classified as first of category-A infectious disease.At present, have the popular FMD of OIE member state of 2/3rds, the moment threatens safety of livestock without FMD countries and regions and Livestock Product Trade.Foot and mouth disease virus belongs to micro ribonucleic acid Viraceae Hostis.Its largest particles diameter is 23 nanometers, and smallest particles diameter is 7-8 nanometer.Current known foot and mouth disease virus has seven principal mode c A, O, C, South Africa 1, South Africa 2, South Africa 3 and Type Asia 1s in the whole world, and more than 65 hypotypes.
O type foot and mouth disease is the popular the widest serotype in the whole world, and the popular foot and mouth disease of China is mainly O, A, C tri-type and ZB type (Baoshan, Yunnan type).According to observations, regional cows, again can be popular in the 1-2 month after effective foot-and-mouth disease vaccine injection, and this often suspects it is caused by another type or subtype virus.This is because this virus easily morphs.The resistance of this virus environment is to external world very strong, and under icy conditions, the virus in blood and feces can be survived 120-170 days.Direct sunlight can kill for lower 60 minutes; Heat 85 DEG C 15 minutes, boiling 3 minutes can be dead.To the susceptible of soda acid, therefore 1%-2% sodium hydroxide, 30% Herba Houttuyniae wood ash, 1%-2% formaldehyde etc. are all good disinfectant solution.
Especially calf is to the most susceptible of foot and mouth disease virus for cattle, and camel, sheep, goat take second place, and pig also can infection morbidity.Primary disease have popular fast, propagate wide, morbidity is anxious, endanger the epidemics such as large, epidemic-stricken area sickness rate can reach 50%-100%, and calf mortality rate is higher, and other are then lower.Ill domestic animal and incubation period animal be the most dangerous source of infection.All containing virus in the vesicle liquid of ill domestic animal, milk, urine, mouth saliva, tear and feces.This sick intrusion feature mainly digestive tract, also can through respiratory tract infection.Though primary disease is propagated without significantly seasonal, and spring and autumn is more, especially spring.Wind and birds are also one of factors of long-distance communications.
This disease 1-7 days incubation period, within average 2-4 days, sick cattle spirit is depressed, and remain silent, sialorrhea, has during opening and suck sound, and body temperature can be elevated to 40-41 DEG C.Fall ill after 1-2 days, sick Dens Bovis seu Bubali gum, lingual surface, inner aspect of lip can see Semen Viciae fabae to the large vesicle of Semen Juglandis, and saliva liquid increases and is that white foam is hung on mouth.Search for food and ruminate stopping.Vesicle is about broken through diel, and form ulcer, at this moment body temperature can be down to normally gradually.To occur in oral cavity while vesicle or after a while, between toe and on the soft skin of coronet, also vesicle to occur, also can very fast ulceration, then heal gradually.Sometimes on skin of nipple, vesicle is also shown.Primary disease generally in optimum process, got final product spontaneous recovery through about one week; If hoof has pathological changes, can be extended down to 2-3 week or more of a specified duration; Mortality rate 1%-2%, this sick type is optimum foot and mouth disease.Some sick cattle is in vesicle agglutination, the state of an illness worsens suddenly, asthenia universalis, muscle are shaken, palpitating speed, irregularity of pulse, appetite is given up absolutely, is ruminated stopping, and walking is waved, astasia, the often sudden death because of cardioplegia, this sick type is pernicious foot and mouth disease, and mortality rate is up to 25% ~ 50%.Often can't see characteristic vesicle during calf morbidity, main manifestations is gastroenteritis haemorrhagica and myocarditis, and mortality rate is high.
Except oral cavity and hoof pathological changes, be also shown in esophagus and cud mucosa has vesicle and speck; Gastrointestinal has hemorrhagic inflammation; Lung is that serosity infiltrates; The a large amount of muddy and liquid of thickness is had in pericardium.Pernicious foot and mouth disease can be seen canescence or faint yellow striped and normal myocardium and go hand in hand on myocardium tangent plane, as Budgerigar shape speckle, is commonly called as " tiger spot heart ".
Foot and mouth disease pathological changes typical case easily identification, therefore be not difficult to make tentative diagnosis in conjunction with clinical disease investigation.Its diagnostic point is:
1. fall ill anxious, popular fast, propagate wide, sickness rate is high, but mortality rate is low, and many in optimum process;
2. a large amount of sialorrhea, in drawing thread shape;
3. mouth hoof skin ulcer location clear and definite (oral mucosa, hoof and skin of nipple), pathological changes special (blister, erosion);
4. visible tiger spot heart during pernicious foot and mouth disease;
5. animal inoculation can be adopted to try fine horse, serodiagnosis and Differential Diagnosis etc. for making a definite diagnosis further.
The difference of foot and mouth disease and Cattle plague: Cattle plague infects fiercely, and mortality rate is high; The visible rotten to the corn but hoof of tumor three-bristle cudrania film is without pathological changes; Gastroenteritis is serious; Abomasum and mucous membrane of small intestine have ulcer.
Foot and mouth disease and malignant catarrh of cattle hot-zone other: the latter often distributes; Oral cavity and nasal mucosa have erosion, but do not form vesicle; The common corneal opacity.(A) fat is controlled foot and mouth disease and should be taked general prevention and treatment measure.Want active prevention at ordinary times, strengthen picking up epidemic disease, regular injections foot-and-mouth disease vaccine is wanted in normal area of sending out.Conventional vaccine has foot and mouth disease attenuated vaccine, hoof-and-mouth disease subunit Seedling and gene engineering vaccine, and cattle produces immunity in 14 days after vaccinate, and immunity can maintain 4--6 month.Once morbidity, then should notify epidemic situation at once, strictly implement the aggregate measures such as blockade, isolation, sterilization, urgent inoculation and treatment simultaneously in epidemic-stricken area; In case of emergency, still foot and mouth disease hyper-immune serum can be applied or rehabilitation animal serum carries out passive immunity, by every kg body weight 0.5-1 milliliter subcutaneous injection, duration of immunity about 2 weeks.Blockade of epidemic focus must in the end 1 ill domestic animal recovery from illness, dead or suddenly to kill latter 14 days, could remove through large sterilization comprehensively.
Suffer from the cattle of optimum foot and mouth disease, generally through multipotency spontaneous recovery in about one week.For shortening the course of disease, preventing secondary infection, can symptomatic treatment: 1. pathological change of oral cavity can use clear water, saline solution or O.1% potassium permanganate liquid cleaning, after be coated with 1% one 2% alum solution or iodine glycerol, also can be coated with and spread the loose (Borneolum Syntheticum 15 grams of Chinese medicine ice coral, Borax 150 grams, 150 grams, Natrii Sulfas, is ground into smalls) in pathological change of oral cavity place; 2. hoof pathological changes can first with a few cleaning of 3% lysol, after embrocate aldrich mixture, iodine glycerol, penicillin ointment etc., bandage; 3. breast lesion can with suds or 2%-3 ~ 3 boric acid water cleaning, after be coated with penicillin ointment.Suffer from the cattle of pernicious foot and mouth disease, except the above-mentioned Local provisions of employing, available cardiac tonic (as caffeine and sodium benzoate) and nourishing agent (as glucose saline) etc.
Conventional control method
(1) when preventing the doubtful foot and mouth disease of ill domestic animal, should report veterinary office immediately, ill domestic animal blocks on the spot, utensil used and pollute ground 2% caustic soda and sterilize.After confirmation, strictly block immediately, isolate, sterilize and a series of activities such as control.Morbidity drove wants harmless treatment after slaughtering, staff goes out to want pasteurised completely, ill domestic animal eats surplus forage or drinking-water, to burn or buried, animal house and near, with 2% caustic soda, dichloro isocyanide globefish acid sodium (containing effective chlorine >=20%), 1% ~ 2% formalin spray disinfectant, in order to avoid loose poison.Inoculation.To cattle and sheep around epidemic-stricken area, select the vaccine that the foot and mouth disease poison type popular with locality is identical, promptly inoculate, consumption, injecting method and points for attention must strictly be pressed vaccine description and be performed.
(2) treat at the beginning of disease, before namely blister appears in oral cavity, with the ill domestic animal haemodialysis of serum or resistance to mistake.Will strengthen feeding and management and nursery work to ill domestic animal, every day washs oral cavity and hoof by saline, boric acid solution etc.Feed with mollugo, soft material or wheat bran medicated porridge etc.When there is ulcer in oral cavity, puts 3 ~ 4 days on the skin with being coated with iodine glycerol mixture (1:1) every day, also can with salty sauce or 10% saline solution.Hoof pathological changes, available disinfectant solution is cleaned, and is coated with gentian violet solution (gentian voiet) or iodine glycerol, and with bandage, can not contacts wetland.
The automatic epidemic method of space electric field
For the epidemic disease of facility and pasture cultivated animals, space electric field biological effect can be utilized to prevent in real time.The space electric field being arranged at environmental safety type poultry house and pasture cultured space can meet the real-time prevention of animal epidemic from three aspects.
(1) space electric field electrode system ionized air produces ozone and the nitric acid gas (nitrogen dioxide) of trace, and ozone can kill the microorganism in air, nitric acid gas has specially killing property to foot and mouth disease virus again, the resistance to Caustic soda of foot and mouth disease virus, quicklime but extremely weak to the acidic materials resistance of pH value 2 ~ 5, nitric acid gas can make its inactivation easily.
(2) space electric field can reduce air pathogen concentration in real time.Space electric field is actually similar to electrostatic field, the output voltage, the electric current that are just applicable to the space electric field generation equipment of preventing epidemic are arranged in the scope to person poultry safety, therefore, it has the air-filtering ability of electrostatic field, air microbe concentration in poultry house at least can be reduced original over half, and then reduce and propagate risk.
(3) space electric field can carry out purification of air and foul smell is cleared up in real time.A large amount of dust or the foul gas such as pellet and ammonia is had in artiodactyls living environment, these pollutant can cause pulmonary infection, conjunctiva inflammation, and then secondary various diseases, and air particle can be removed according to electrostatic force at space electric field, and foul gas can be decomposed into harmless micromolecule again by ionized air.
The automatic epidemic method of space electric field is mainly used in the safe construction of environment in intensive livestock-raising field, guarantees that animal exempts from the propagation of sudden strong epidemic disease, reduces the use of veterinary drug to greatest extent, guarantee the bio-safety of breeding environment.
Summary of the invention
Technical problem to be solved by this invention is for the deficiency of disinfectant of the prior art existing for kill in foot and mouth disease virus and provides a kind of natural acid disinfectant preparing the application killed in hoof-and-mouth disease toxin preparation.
Technical problem to be solved by this invention can be achieved through the following technical solutions:
Natural acid disinfectant is preparing the application killed in hoof-and-mouth disease toxin preparation, and the total fatty acid content in wherein said natural acid disinfectant is 9.9wt% ~ 11.9wt%.
In a preferred embodiment of the invention, described natural acid disinfectant union is sour, sad, capric acid and solvent formulated, wherein the content of n-nonanoic acid is 4.6wt% ~ 5.5wt%, sad content is 3.1wt% ~ 3.7wt%, capric acid 2.1wt% ~ 2.7wt%.
In a preferred embodiment of the invention, described solvent is dodecylbenzene sodium sulfonate.
Natural acid disinfectant of the present invention is 100% to the killing rate of foot and mouth disease virus.
Detailed description of the invention
Natural acid disinfectant is preparing the application killed in hoof-and-mouth disease toxin preparation, and the total fatty acid content in wherein said natural acid disinfectant is 9.9wt% ~ 11.9wt%.This fatty acid disinfectant union is sour, sad, capric acid and solvent formulated, wherein the content of n-nonanoic acid is 4.6wt% ~ 5.5wt%, sad content is 3.1wt% ~ 3.7wt%, capric acid 2.1wt% ~ 2.7wt%.Solvent is dodecylbenzene sodium sulfonate.
Natural acid disinfectant of the present invention is to the detection of foot and mouth disease virus killing effect, and result of the test is reported as follows:
1. test material and preparation
1.1 natural acid disinfectant total fatty acid contents are 9.9wt% ~ 11.9wt%, are packaged as 100ml/ bottle.
1.2 diluents: 0.04mol/L phosphate buffer (pH7.6), sterilizing deionized water.
The preparation of 1.3 viruses and dilution: O type foot and mouth disease neonatal rat tissue poison (LD
50=10
-8.0), be diluted to 1: 500 times of viral suspension with 0.04mol/L phosphate buffer (pH7.6), for subsequent use.
1.4 experimental animal
4 ages in days are sucked the breast white mice (neonatal rat), are provided by Lanzhou Veterinary Inst., Chinese Acedemy of Agaricultural Sciences's laboratory animal field.
2. test method
2.1 safety testing
With deionized water, fatty acid disinfectant solution of the present invention is done 1: 50,1: 100,1: 200 times of dilution, inject 4 age in days neonatal rats respectively, every neonatal rat dorsal sc injection 0.2ml disinfectant solution diluent, often organize injection 5; If negative control, negative control often organizes 5 neonatal rats, every neonatal rat dorsal sc injection 0.2ml deionized water, Continuous Observation 72h after injection, often group test repetition 4 times.
2.2 tests killing foot and mouth disease virus effect
1: 100,1: 200,1: 300,1: 350 and 1: 400 times of dilution is done by deionized water natural acid disinfectant just of the present invention, the foot and mouth disease virus mixed in equal amounts of diluting with 1: 500 times respectively, 4 age in days neonatal rats are injected after room temperature effect 30min, often organize 5, every neonatal rat dorsal sc injection virus-disinfectant solution mixed liquor 0.2ml; If virus control (positive control), virus control often organizes 5 neonatal rats, the disinfectant solution of every neonatal rat dorsal sc injection 0.2ml 1: 1000 times of dilution; If normal healthy controls (blank), normal healthy controls often organizes 5 neonatal rats, every neonatal rat dorsal sc injection 0.2ml deionized water, Continuous Observation 7 after injection.Often group test repetition 5 times.
3. criterion
3.1 safety standards, neonatal rat is not dead or have no adverse reaction, and is judged to safety, otherwise is dangerous.
3.2 kill virus effect criterion
(1) virus control group neonatal rat is dead in virus inoculation sequela, and foot and mouth disease virus detected in neonatal rat body, and test is set up.
(2) test group neonatal rat is in viewing duration performance without exception, and whole key is lived, and judges that product is effective.
(3) test group has neonatal rat dead, and detect in neonatal rat body foot and mouth disease virus judge this concentration product invalid.
4. result of the test
4.1 natural acid disinfectant of the present invention at 1: 100 times and when diluting above, to laboratory animal safety.
The results are shown in Table 1
Table 1: disinfectant is to the Security test of animal
4.2 natural acid disinfectant of the present invention, to viral kill effect measuring, the results are shown in Table 2
Table 2 natural acid disinfectant is to the mensuration of foot and mouth disease virus killing effect
5. conclusion (of pressure testing)
5.1 natural acid disinfectant of the present invention can kill foot and mouth disease virus.
It is 1: 700 that 5.2 natural acid disinfectant 100% of the present invention kill the most high dilution of foot and mouth disease virus.
Claims (3)
1. natural acid disinfectant is preparing the application killed in hoof-and-mouth disease toxin preparation, and the total fatty acid content in wherein said fatty acid disinfectant is 9.9wt% ~ 11.9wt%.
2. apply as claimed in claim 1, it is characterized in that, described natural acid disinfectant union is sour, sad, capric acid and solvent formulated, wherein the content of n-nonanoic acid is 4.6wt% ~ 5.5wt%, sad content is 3.1wt% ~ 3.7wt%, capric acid 2.1wt% ~ 2.7wt%.
3. apply as claimed in claim 1 or 2, it is characterized in that, described solvent is dodecylbenzene sodium sulfonate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018002372A1 (en) * | 2016-06-30 | 2018-01-04 | Devenish Nutrition Limited | A composition for use in treating rotavirus infection |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010138162A (en) * | 2008-11-12 | 2010-06-24 | Bussan Biotech Kk | Anti-prrs virus agent and its utilization |
| CN102159181A (en) * | 2008-05-06 | 2011-08-17 | 马拉德克里科聚合物公司 | Cationic latex as carrier for active ingredients and methods for making and using same |
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2015
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102159181A (en) * | 2008-05-06 | 2011-08-17 | 马拉德克里科聚合物公司 | Cationic latex as carrier for active ingredients and methods for making and using same |
| JP2010138162A (en) * | 2008-11-12 | 2010-06-24 | Bussan Biotech Kk | Anti-prrs virus agent and its utilization |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018002372A1 (en) * | 2016-06-30 | 2018-01-04 | Devenish Nutrition Limited | A composition for use in treating rotavirus infection |
| JP2019524696A (en) * | 2016-06-30 | 2019-09-05 | デベニッシュ ニュートリション リミテッド | Composition for use in the treatment of rotavirus infection |
| US11344523B2 (en) | 2016-06-30 | 2022-05-31 | Devenish Nutrition Limited | Composition for use in treating rotavirus infection |
| AU2017291276B2 (en) * | 2016-06-30 | 2023-06-15 | Devenish Nutrition Limited | A composition for use in treating rotavirus infection |
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Application publication date: 20151104 |
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