CN104990673A - Pipettor close performance detection device - Google Patents
Pipettor close performance detection device Download PDFInfo
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- CN104990673A CN104990673A CN201510487410.8A CN201510487410A CN104990673A CN 104990673 A CN104990673 A CN 104990673A CN 201510487410 A CN201510487410 A CN 201510487410A CN 104990673 A CN104990673 A CN 104990673A
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Abstract
The invention relates to a pipettor close performance detection device. The detection device is formed by a transparent reagent bottle, a glass syringe, a right latex tube, a right three-way valve, a right glass tube, a vacuum pressure gauge, a left glass tube, a left three-way valve, a left latex tube, a liquid-absorbing nozzle and a soft rubber bottle plug. A pipettor close performance detection method of the pipettor close performance detection device is characterized by adjusting a pipettor to be detected to nominal capacity; installing the liquid-absorbing nozzle to the pipettor to be detected and screwing the liquid-absorbing nozzle tightly; carrying out connection according to the installation connection step; adjusting rotary knobs of the left and right three-way valves and extracting air with the glass syringe and repeating the operation until a pointer of the vacuum pressure gauge points to -0.04 MPa; and carrying out close performance detection of the pipettor to be detected through a closed loop formed by the pipettor to be detected, the left three-way valve, the transparent reagent bottle, the vacuum pressure gauge and the b end of the right three-way valve in sequence. The detection device solves the problems that it is troublesome and not easy to carry out the detection and efficiency is not high when carrying out close performance detection on the pipettor.
Description
Technical field
The invention belongs to inspection fixture fields, particularly a kind of verifying attachment of pipettor adaptation.
Background technology
Pipettor is mainly used in the scientific research departments such as environmental protection, medicine, food hygiene, is used as sampling or the liquid feeding of liquid in biochemical analysis and chemical examination.It is a piston type suction pipe, utilizes air venting principle to carry out work, with the capacity of piston distance determination pipettor of movement in piston bush.The adaptation of pipettor refers to get in liquid, discharge opeing process at a complete liquid, pipettor piston and be transferred the relative vacuum degree of the confined space formed between liquid.JJG646-2006 " pipettor " measurement verification regulations the 6.5th pair of pipettor adaptation has made clear and definite generic specifications, and when the 7.2nd Interventions Requested regulation " first calibrating " and " follow-up calibrating ", adaptation is emergencing testeding project.At present, nearly all pipettor manufacturer does not all test to the adaptation of pipettor before product export He in user's use procedure.In fact, the accuracy problem ubiquity moving liquid result caused because adaptation is defective, analyzes according to statistics, and the accuracy problem reaching the pipettor of more than 80% causes because adaptation is defective.
Summary of the invention
For overcoming above-mentioned defect, the object of this invention is to provide the pipettor adaptation verifying attachment that a kind of structure is simple, easy to operate and with low cost.
A kind of pipettor adaptation verifying attachment, this verifying attachment is made up of transparent agent bottle, glass syringe, right emulsion tube, right T-valve, right glass tube, pressure vacuum gauge, left glass tube, left T-valve, left emulsion tube, imbibition nozzle, soft rubber bottle stopper; The bottleneck of described soft rubber bottle stopper and transparent agent bottle is wringing fit, and it is fixed on the bottleneck of transparent agent bottle; Pressure vacuum gauge is fixed on the centre of soft rubber bottle stopper, for measuring the vacuum tightness in transparent agent bottle; Right glass tube, left glass tube are oppositely arranged on the two ends of pressure vacuum gauge; One end of right glass tube is end of bleeding, and it inserts transparent agent bottle inner chamber through soft rubber bottle stopper, and the other end is connected with right T-valve; One end of right emulsion tube is connected with right T-valve, and the other end is connected with glass syringe; Right T-valve is between right emulsion tube, right glass tube.One end of left glass tube is test lead, and it inserts transparent agent bottle through soft rubber bottle stopper and immerses in distilled water, and the other end of left glass tube is connected with left T-valve; One end of left emulsion tube is connected with left T-valve, and the other end to be sleeved on imbibition nozzle and to be connected with pipettor to be checked; Left T-valve is between left glass tube, left emulsion tube; In transparent agent bottle, fill distilled water, the height of distilled water liquid level is 3/5 of transparent agent bottle height degree; Right emulsion tube, right glass tube, left glass tube, left emulsion tube be shape all at right angles; Right T-valve, left T-valve are adjustable T-valve.
The method adopting pipettor adaptation verifying attachment of the present invention to carry out the inspection of pipettor adaptation is: pipettor to be checked is transferred to nominal capacity, imbibition nozzle is installed on pipettor to be checked and screws, connect according to installation Connection Step, step 1: rotate left T-valve knob, closing a makes b, c connect, rotate right T-valve knob, close a and b, c are connected; Use glass syringe exhaust, if pressure vacuum gauge does not reach predetermined pressure; Step 2: then rotate right T-valve knob closedown b and a, c are connected, glass syringe piston is got back to initial position, then rotate right T-valve knob close a b, c are connected, use glass syringe exhaust, repeat step 2 to operate, until vacuum pressure list index is indicated to-0.04MPa; Rotate right T-valve knob and close b, after holding the loop formed to reach balance by the b of pipettor to be checked-left T-valve-transparent agent bottle-pressure vacuum meter-right T-valve, continue 5 s, now observation test end lower end, as adaptation is good, then do not have bubble in distilled water to produce, pipettor adaptation inspection to be checked meets the requirements; Otherwise then have continuous air bubbles in distilled water and occur, pipettor adaptation inspection to be checked is undesirable.
The size of described transparent agent bottle is 500 ~ 1000mL.
The dial plate diameter of described pressure vacuum gauge is 40mm, and measurement range is 0 ~-0.1MPa, and class of accuracy is 1.6 grades.
Described test lead immerses between the distillation water surface 30 ~ 50mm; Described end of bleeding is between the distilled water water surface 15 ~ 20mm.
Verifying attachment of the present invention solves pipettor when carrying out adaptation inspection, both bothers, the not easy to operate and problem that efficiency is not high.Both improve the product percent of pass of pipettor, turn improved production efficiency, be applicable to very much the needs of manufacturing enterprise, applying unit and metrological service.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of pipettor adaptation verifying attachment of the present invention;
Fig. 2 is the structural representation of soft rubber bottle stopper in pipettor adaptation verifying attachment of the present invention;
Fig. 3 is pipettor adaptation inspection process figure of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail.
As shown in Figure 1 and Figure 2, a kind of pipettor adaptation verifying attachment, this device is made up of transparent agent bottle 1, glass syringe 2, right emulsion tube 3, right T-valve 4, right glass tube 5, pressure vacuum gauge 6, left glass tube 7, left T-valve 8, left emulsion tube 9, imbibition nozzle 10, soft rubber bottle stopper 12.Soft rubber bottle stopper 12 is wringing fit with the bottleneck of transparent agent bottle 1, and it is fixed on the bottleneck of transparent agent bottle 1.Pressure vacuum gauge 6 is fixed on the centre of soft rubber bottle stopper 12, for measuring the vacuum tightness in transparent agent bottle 1.Right glass tube 5, left glass tube 7 are oppositely arranged on the two ends of pressure vacuum gauge 6.One end of right glass tube 5 is end 14 of bleeding, and it inserts the transparent agent bottle 1 inner chamber distance distillation water surface 15 ~ 20mm through soft rubber bottle stopper 12, and the other end is connected with right T-valve 4.One end of right emulsion tube 3 is connected with right T-valve 4, and the other end is connected with glass syringe 2.Right T-valve 4 is between right emulsion tube 3, right glass tube 5.One end of left glass tube 7 is test lead 13, and it inserts transparent agent bottle 1 through soft rubber bottle stopper 12 and immerses distilled water 30 ~ 50mm, and the other end of left glass tube 7 is connected with left T-valve 8; One end of left emulsion tube 9 is connected with left T-valve 8, and the other end to be sleeved on imbibition nozzle 10 and to be connected with pipettor 11 to be checked.Left T-valve 8 is between left glass tube 7, left emulsion tube 9.In transparent agent bottle 1, fill distilled water, the height of distilled water liquid level is 3/5 of transparent agent bottle height degree.Right emulsion tube 3, right glass tube 5, left glass tube 7, left emulsion tube 9 be shape all at right angles.Right T-valve 4, left T-valve 8 are adjustable T-valve.The size of transparent agent bottle 1 is 500 ~ 1000mL.The dial plate diameter of pressure vacuum gauge 6 is 40mm, and measurement range is 0 ~-0.1MPa, and class of accuracy is 1.6 grades.
The method for making of pipettor adaptation verifying attachment of the present invention:
(1) hole: with transparent agent bottle 1 supporting soft rubber bottle stopper 12 center for the center of circle, boring a diameter with boring apparatus is φ 16 ~ 18mm macropore; Radially a diameter is respectively bored on distance left and right both sides, 25mm place, macropore center is again φ 2 ~ 4mm aperture.Position, large and small hole schematic diagram as shown in Figure 2.
(2) connection is installed:
The first step: pressure vacuum gauge 6 is arranged on large hole site and tightens, mounted pressure vacuum gauge 6 should be vertical with soft rubber bottle stopper 12;
Second step: 90o is bent right glass tube 5, left glass tube 7 is arranged on left and right 2 aperture position of soft rubber bottle stopper 12 respectively;
3rd step: be 1. connected with the b end of T-valve 8 with glass tube 7 upper end, then with left emulsion tube 9, the c of T-valve 8 end be connected with pipettor imbibition nozzle 10;
4th step: glass tube 5 upper end is connected with the b end of T-valve 4, then is connected with glass syringe 2 by the c of T-valve 4 end with right emulsion tube 3;
5th step: distilled water is injected in transparent agent bottle 1, liquid level is 3/5 of reagent bottle height;
6th step: cover tightly soft rubber bottle stopper 12, regulates test lead 13 glass tube lower end, makes it immerse distilled water 50mm place, and end 14 glass tube lower end of bleeding is adjusted to apart from distilled water water surface 20mm place; Tighten each joint and stationary installation.
Adopting the present invention to carry out pipettor adaptation checks the method for self-inspection as follows:
Step 1: swivel tee valve 8 knob, close b, partition pipettor and transparent agent bottle 1 communicate, and rotate right T-valve 4 knob, closing a makes b, c connect, and forms a loop be made up of the b end-transparent agent bottle 1-pressure vacuum meter 6-right T-valve 4-glass syringe 2 of left T-valve 8.With glass syringe 2 exhaust, if pressure vacuum gauge 6 does not reach predetermined pressure; Step 2: then rotate right T-valve 4 knob closedown b and a, c are connected, glass syringe 2 piston is got back to initial position; Rotating right T-valve 4 knob closedown a again makes b, c connect, with glass syringe 2 exhaust ... repeat step 2 to operate, until vacuum pressure list index is indicated to-0.04MPa.Rotate right T-valve 4 knob and close b, pressure vacuum gauge 6 indicated value does not change at the appointed time, then prove that the self-inspection of instrument adaptation is qualified, can carry out the inspection of pipettor adaptation.
The method that employing the present invention carries out the acceptance test of pipettor adaptation is as follows:
Pipettor 11 to be checked is transferred to nominal capacity, imbibition nozzle 10 is installed on pipettor 11 to be checked and screws, connect according to installation Connection Step, step 1: rotate left T-valve 8 knob, close a and b, c are connected, rotate right T-valve 4 knob, close a and b, c are connected.With glass syringe 2 exhaust, if pressure vacuum gauge 6 does not reach predetermined pressure, step 2: then rotate right T-valve 4 knob closedown b and a, c are connected, glass syringe 2 piston is got back to initial position, rotating right T-valve 4 knob closedown a again makes b, c connect, with glass syringe 2 exhaust ... repeat step 2 to operate, until pressure vacuum gauge 6 pointer is indicated to-0.04MPa.Rotate right T-valve 4 knob and close b, after holding the loop formed to reach balance by the b of the right T-valve 4 of pipettor 11-to be checked left T-valve 8-transparent agent bottle 1-pressure vacuum meter 6-, continue 5 s, now observation test end 13 glass tube lower end, as adaptation is good, then do not have bubble to produce, pipettor 11 adaptation inspection to be checked meets the requirements; Otherwise then have continuous air bubbles and occur, pipettor adaptation inspection to be checked is undesirable.
Claims (4)
1. a pipettor adaptation verifying attachment, is characterized in that: this verifying attachment is made up of transparent agent bottle (1), glass syringe (2), right emulsion tube (3), right T-valve (4), right glass tube (5), pressure vacuum gauge (6), left glass tube (7), left T-valve (8), left emulsion tube (9), imbibition nozzle (10), soft rubber bottle stopper (12); Described soft rubber bottle stopper (12) is wringing fit with the bottleneck of transparent agent bottle (1), and it is fixed on the bottleneck of transparent agent bottle (1); Pressure vacuum gauge (6) is fixed on the centre of soft rubber bottle stopper (12), for measuring the vacuum tightness in transparent agent bottle (1); Right glass tube (5), left glass tube (7) are oppositely arranged on the two ends of pressure vacuum gauge (6); One end of right glass tube (5) is end (14) of bleeding, and it inserts transparent agent bottle (1) inner chamber through soft rubber bottle stopper (12), and the other end is connected with right T-valve (4); One end of right emulsion tube (3) is connected with right T-valve (4), and the other end is connected with glass syringe (2); Right T-valve (4) is positioned between right emulsion tube (3), right glass tube (5); One end of left glass tube (7) is test lead (13), and it inserts transparent agent bottle (1) through soft rubber bottle stopper (12) and immerses in distilled water, and the other end of left glass tube (7) is connected with left T-valve (8); One end of left emulsion tube (9) is connected with left T-valve (8), and the other end is sleeved on imbibition nozzle (10) and goes up and be connected with pipettor to be checked (11); Left T-valve (8) is positioned between left glass tube (7), left emulsion tube (9); In transparent agent bottle (1), fill distilled water, the height of distilled water liquid level is 3/5 of transparent agent bottle height degree; Right emulsion tube (3), right glass tube (5), left glass tube (7), left emulsion tube (9) be shape all at right angles; Right T-valve (4), left T-valve (8) are adjustable T-valve;
The method adopting above-mentioned pipettor adaptation verifying attachment to carry out the inspection of pipettor adaptation is: pipettor to be checked (11) is transferred to nominal capacity, imbibition nozzle (10) is installed on pipettor to be checked (11) and screws, connect according to installation Connection Step, step 1: rotate left T-valve (8) knob, closing a makes b, c connect, rotate right T-valve (4) knob, close a and b, c are connected; With glass syringe (2) exhaust, if pressure vacuum gauge (6) does not reach predetermined pressure; Step 2: then rotate right T-valve (4) knob closedown b and a, c are connected, glass syringe (2) piston is got back to initial position, rotating right T-valve (4) knob closedown a again makes b, c connect, with glass syringe (2) exhaust, repeat step 2 to operate, until pressure vacuum gauge (6) pointer is indicated to-0.04MPa; Rotate right T-valve (4) knob and close b, after holding the loop formed to reach balance by the b of pipettor to be checked (11)-left T-valve (8)-transparent agent bottle (1)-pressure vacuum meter (6)-right T-valve (4), continue 5s, now observation test end (13) lower end, as adaptation is good, then do not have bubble in distilled water to produce, the inspection of pipettor to be checked (11) adaptation meets the requirements; Otherwise then have continuous air bubbles in distilled water and occur, the inspection of pipettor to be checked (11) adaptation is undesirable.
2. pipettor adaptation verifying attachment according to claim 1, is characterized in that: the size of described transparent agent bottle (1) is 500 ~ 1000 mL.
3. pipettor adaptation verifying attachment according to claim 1, is characterized in that: the dial plate diameter of described pressure vacuum gauge (6) is 40mm, and measurement range is 0 ~-0.1MPa, and class of accuracy is 1.6 grades.
4. pipettor adaptation verifying attachment according to claim 1, is characterized in that: described test lead (13) immerses between the distillation water surface 30 ~ 50mm; Described end (14) of bleeding is between the distilled water water surface 15 ~ 20mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510487410.8A CN104990673A (en) | 2015-08-11 | 2015-08-11 | Pipettor close performance detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510487410.8A CN104990673A (en) | 2015-08-11 | 2015-08-11 | Pipettor close performance detection device |
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| CN104990673A true CN104990673A (en) | 2015-10-21 |
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Cited By (4)
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| CN105699027A (en) * | 2016-01-28 | 2016-06-22 | 遵义市产品质量检验检测院 | A multichannel pipettor sealing performance examination device |
| CN106323558A (en) * | 2016-07-29 | 2017-01-11 | 广东小天才科技有限公司 | Waterproof test method and system |
| CN107036764A (en) * | 2017-06-08 | 2017-08-11 | 南京医科大学 | A kind of High Precision Automatic hand-held pipettor metering device and its metering method |
| CN114414722A (en) * | 2022-01-18 | 2022-04-29 | 天脊煤化工集团股份有限公司 | Method for measuring content of carbon dioxide in phosphorite |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107036764A (en) * | 2017-06-08 | 2017-08-11 | 南京医科大学 | A kind of High Precision Automatic hand-held pipettor metering device and its metering method |
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Application publication date: 20151021 |
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