CN104988185B - The method that Trichoderma Viride extract improves alcoholic fermentation yield - Google Patents
The method that Trichoderma Viride extract improves alcoholic fermentation yield Download PDFInfo
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- CN104988185B CN104988185B CN201510164803.5A CN201510164803A CN104988185B CN 104988185 B CN104988185 B CN 104988185B CN 201510164803 A CN201510164803 A CN 201510164803A CN 104988185 B CN104988185 B CN 104988185B
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- 238000000855 fermentation Methods 0.000 title claims abstract description 55
- 230000004151 fermentation Effects 0.000 title claims abstract description 53
- 230000001476 alcoholic effect Effects 0.000 title claims abstract description 26
- 241000223261 Trichoderma viride Species 0.000 title claims abstract description 16
- 239000000284 extract Substances 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 title claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 76
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 35
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 17
- 239000010902 straw Substances 0.000 claims abstract description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 12
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229920002472 Starch Polymers 0.000 claims abstract description 8
- 235000019698 starch Nutrition 0.000 claims abstract description 8
- 239000008107 starch Substances 0.000 claims abstract description 8
- 239000000758 substrate Substances 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 4
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 4
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 4
- 239000001110 calcium chloride Substances 0.000 claims abstract description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 4
- 238000001556 precipitation Methods 0.000 claims abstract description 4
- 238000010563 solid-state fermentation Methods 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 239000010959 steel Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims 1
- 239000003643 water by type Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 description 54
- 230000012010 growth Effects 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000012530 fluid Substances 0.000 description 5
- 239000012752 auxiliary agent Substances 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 235000017190 Vitis vinifera subsp sylvestris Nutrition 0.000 description 1
- 244000237969 Vitis vulpina Species 0.000 description 1
- 235000017242 Vitis vulpina Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009604 anaerobic growth Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940066779 peptones Drugs 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000010907 stover Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a kind of methods that Trichoderma Viride extract improves alcoholic fermentation yield, include at least following steps：（1）Solid state fermentation is carried out by raw material of stalk, straw powder is added in fermenter, adds water and ammonium nitrate, and be sufficiently stirred, then accesses Trichoderma viride CGMCC3.6619, strain is made to be come into full contact with straw powder；Continuous culture one month, dries, it is spare to obtain fermentation substrate after fermentation；（2）The extraction and purifying that extract alcohol rises are filtered after constant temperature processing with NaOH solution soaking fermentation matrix, stoste are obtained after concentrating filter liquor；Then stoste is adjusted to pH 2.0, is stood, centrifugation, is removed precipitation, product alcohol liter is obtained after clear liquid is spray-dried；（3）Alcoholic fermentation takes starch that clear water is added, and stirring makes starch be uniformly dispersed in water, and liquid alpha-amylase is then added, and calcium chloride adjusts pH, adds liquid saccharifying enzyme, saccharification terminates postcooling, and activated saccharomyces cerevisiae and alcohol liter is added, and ferments in thermostatic chamber.
Description
Technical field
The invention belongs to prepare biologically active mixture by microorganism fungus kind to improve alcoholic fermentation yield
Method.
Background technology
Fuel alcohol（Ethyl alcohol）It is one of three big new energy of 21 century, and high-concentration alcoholic fermentation is the certainty of industry development
One of trend and technical bottleneck improve yeast to the tolerance of alcohol in high concentration to improve yeast using reasonable process condition
Production alcohol ability receives significant attention.The factor for influencing high-concentration alcoholic fermentation is diversified, and restricted for these
The countermeasure of the factor, people is also by every possible means.In the initiative for carrying out breeding species, improved equipment, Optimal Medium etc.
On Research foundation, someone is conceived to the efficient utilization of existing equipment and technique, using complete batch fermentation mode, realizes high concentration
Alcoholic fermentation.Also there is researcher by adding substance necessary to the increment of saccharomycete anaerobic growth into fermentation liquid, increasing is provided
The substance that strong yeast cell resists poor environment factor finally realizes alcohol in high concentration to optimize the composition of fermentation liquid
Fermentation.
Currently, for alcoholic fermentation auxiliary agent because its is easy to use, better results and is well received by the public in the market.Intelligent dove et al. is bent to find
The fermentation assistant of French Lallemand companies production can accelerate the course of fermentation of wild grape wine, improve the conversion ratio of sugar.But its
Higher price, and total acid content increased after use.Alcoholic fermentation auxiliary agent further includes surfactant such as Tween-20, or
Person's yeast nutritire salt such as Angel fermentation accelerant, for improving the alcohol concentration in fermentation liquid.Also it very ferments synergy just like ferment
Agent and Fermentation Additive in Liquor Production etc. can improve alcoholic fermentation efficiency, but because production cost height and product using effect are limited, very
It is rare to be used widely in actual production.
Invention content
The present invention is in view of the above-mentioned problems, provide a kind of method that Trichoderma Viride extract improves alcoholic fermentation yield.
The method that a kind of Trichoderma Viride extract of the present invention improves alcoholic fermentation yield includes at least as follows
Step：
（1）Solid state fermentation is carried out by raw material of stalk
Straw powder is added in fermenter, adds water and ammonium nitrate, and be sufficiently stirred, then accesses Trichoderma viride
CGMCC3.6619 makes strain be come into full contact with straw powder；It continuously cultivates one month in 28 DEG C of thermostatic chambers, does after fermentation
It is dry, it is spare to obtain fermentation substrate；
（2）The extraction and purifying that extract alcohol rises
The NaOH solution leach step for being 3.5% with mass fraction（1）Obtained drying for standby fermentation substrate, 60 DEG C of constant temperature
It is filtered after water-bath 1h, stoste is obtained after concentrating filter liquor；Then stoste is adjusted to pH 2.0, after standing 10 min with hydrochloric acid
15min is centrifuged through 4 000rpm, removal precipitation obtains alcohol and rises product after spray-dried；
（3）Alcoholic fermentation
It takes starch 1kg to be put into stainless cylinder of steel, clear water 3L is added, stirring makes starch be uniformly dispersed in water, is then added
Liquid alpha-amylase 1mL, calcium chloride 1g, 6.5 are adjusted to by the pH of system, and feed liquid is heated to 90 DEG C, keeps the temperature 30min postcoolings
To 60 DEG C, regulation system pH to 4.5,2mL liquid saccharifying enzymes are added, 60 DEG C of saccharification 60min is maintained, is cooled to after saccharification
30 DEG C, and activated saccharomyces cerevisiae and alcohol liter 15g is added, ferment 48h in 28 DEG C of thermostatic chambers, obtains fermentation liquid, measures hair
Alcohol concentration in ferment mash is 17%（v/v）.
Further, straw powder fresh paddy rice stalk crushes after air-drying, and crosses 1mm sieves.
Further, the straw powder, water, ammonium nitrate ratio be：Straw powder 1kg, water 3kg, ammonium nitrate 10g；Institute
The Trichoderma viride CGMCC3.6619 inoculum concentrations stated are the 1% of stalk dry weight.
The beneficial effects of the invention are as follows：The present invention prepares alcohol liter by raw material of stalk, is then added to alcoholic fermentation culture
In base, to improve yeast tolerance, increase alcohol output.Alcohol liter is easy to use, is carried while reducing the production cost of alcohol
High alcohol output, using effect is apparent, is a kind of alcoholic fermentation auxiliary agent really with commercial application value.Except this it
Outside, it significantly improves the biotransformation efficiency of matrix also by addition efficient degrading bacterial strain Trichoderma viride CGMCC3.6619, this
So that stover rate is up to 75% or more during preparing alcohol and rising, transformation efficiency and fermenting speed are leading, and obtain
It is cheap that the alcohol arrived rises product price.
Description of the drawings
Fig. 1 is influence of the various concentration alcohol liter to yeast growth（107Ge ∕ ml）Figure.
Fig. 2 is influence diagram of the various concentration alcohol liter to yeast producing and ethanol.
Fig. 3 is influence table of the various concentration alcohol liter to glucose consumption.
Specific implementation mode
The invention will be further described with reference to the accompanying drawings and examples.
As shown in the figure：A kind of Trichoderma Viride extract of the present invention improves the method for alcoholic fermentation yield at least
Include the following steps：
（1）Solid state fermentation is carried out by raw material of stalk
Straw powder is added in fermenter, adds water and ammonium nitrate, and be sufficiently stirred, then accesses Trichoderma viride
CGMCC3.6619 makes strain be come into full contact with straw powder；It continuously cultivates one month in 28 DEG C of thermostatic chambers, does after fermentation
It is dry, it is spare to obtain fermentation substrate；
（2）The extraction and purifying that extract alcohol rises
The NaOH solution leach step for being 3.5% with mass fraction（1）Obtained drying for standby fermentation substrate, 60 DEG C of constant temperature
Water-bath is filtered after 1 hour, and stoste is obtained after concentrating filter liquor；Then stoste is adjusted to pH 2.0 with hydrochloric acid, stand 10 min
It is centrifuged 15 minutes by 4 000 revs/min, removal precipitation obtains alcohol and rises product after spray-dried；
（3）Alcoholic fermentation
It takes starch 1kg to be put into stainless cylinder of steel, clear water 3L is added, stirring makes starch be uniformly dispersed in water, is then added
Liquid alpha-amylase 1mL, calcium chloride 1g, 6.5 are adjusted to by the pH of system, and feed liquid is heated to 90 DEG C, keeps the temperature 30min postcoolings
To 60 DEG C, regulation system pH to 4.5,2mL liquid saccharifying enzymes are added, 60 DEG C of saccharification 60min is maintained, is cooled to after saccharification
30 DEG C, and activated saccharomyces cerevisiae and alcohol liter 15g is added, ferment 48h in 28 DEG C of thermostatic chambers, obtains fermentation liquid, measures hair
Alcohol concentration in ferment mash is 17%（v/v）.
Straw powder fresh paddy rice stalk crushes after air-drying, and crosses 1mm sieves.
The straw powder, water, ammonium nitrate ratio be：Straw powder 1kg, water 3kg, ammonium nitrate 10g；The green wood
Mould CGMCC3.6619 inoculum concentrations are the 1% of stalk dry weight.
Alcohol rises the influence experiment to yeast growth speed：
YPD fluid nutrient mediums are prepared, contain 20g peptones, 20g glucose, 10g yeast extracts in every 1L water.Additional addition
The amount that alcohol rises presses concentration 0g/L, 0.1g/L, 0.2g/L, 0.4g/L, 0.5g/L, 1g/L, 1.25g/L, 1.7g/L, 2.5g/L, 5g/
L, 10g/L are added in shaking flask.It is inoculated with equivalent yeast liquid spawn, the constant temperature incubation at 170r/min on shaking table, 28 DEG C, per 1h
Its primary yeast quantity of detection and OD values are to analyze influence of the alcohol liter to yeast growth speed.
Metabolite when alcohol is yeast growth, yeast growth situation have decisive influence, logarithm life to alcoholic fermentation
Long-term yeast production alcohol ability is most strong.Therefore, yeast number amount changes in zymotic fluid when determining anaerobic fermentation, investigates different dense
Spend influence of the alcohol liter to yeast growth.The result shows that（Fig. 1）, alcohol liter has a significant impact the variation of yeast bio amount, with hair
Alcohol rises the increase of dosage in zymotic fluid, and yeast quantity accordingly increases, also, Yeast proliferation speed improves, and shortens laundering period, logarithm
Growth period shifts to an earlier date.When the dosage that alcohol rises reaches 5.0 g/L, yeast bio amount can increase by 100%, and maximum biomass shifts to an earlier date 2
d；When the dosage that alcohol rises reaches 10.0 g/L, yeast bio amount is not further added by.
Alcohol rises the influence experiment to yeast producing and ethanol and ethanol tolerance power：
Concentration of glucose is prepared up to 25% fermentation medium, packing 280mL or so fermented and cultureds are based on 250mL specifications
In shaking flask（Culture medium liquid level is from bottleneck about 1cm）, the alcohol of addition, which rises, presses setting concentration 0g/L, 0.1g/L, 0.5g/L, 1.65g/L,
2.5g/L, 3.3g/L, 5g/L, 10g/L are added in fermentation flask, and insulating box 28 is statically placed in after the yeast liquid of inoculation equivalent activation
DEG C anaerobic fermentation, every detecting zymotic fluid yeast quantity in primary each fermentation flask, glucose residual, alcoholic strength and last for 24 hours
The attainable maximum alcoholic strength of institute, to analyze influence of the alcohol liter to yeast producing and ethanol and resistance to ethyl alcohol.
The key of high-concentration alcoholic fermentation is the Commpensation And Adaptation for solving yeast to ethyl alcohol.Result of study shows various concentration
Alcohol rise and have a facilitation to yeast producing and ethanol, alcohol concentration is significantly increased in zymotic fluid.Reach 5.0 when alcohol rises dosage
When g/L, highest alcohol concentration can be increased to 15.8% from 7.1%, and alcohol content increases 123%.And increases alcohol and rise dosage extremely
10.0 g/L, highest alcohol concentration are not further added by（Fig. 2）.It follows that alcohol liter produces alcohol to yeast and ethanol tolerance ability is equal
It is obviously improved effect.
By the examination of glucose concentration to fermentation process, the consumption of glucose and the increase of yeast bio amount are couplings
, when yeast growth speed is fast, the depletion rate of glucose is also fast, and yeast bio amount increased even more, the amount of glucose consumption
Also more（Fig. 3）.Change in conjunction with alcohol concentration and find, yeast growth also will continue to fermentation production alcohol, and highest wine after stopping
The time that smart concentration occurs is later than yeast maximum biomass 2d.
Claims (3)
1. a kind of method that Trichoderma Viride extract improves alcoholic fermentation yield, it is characterised in that include the following steps：
（1）Solid state fermentation is carried out by raw material of stalk
Straw powder is added in fermenter, adds water and ammonium nitrate, and be sufficiently stirred, then accesses Trichoderma viride
CGMCC3.6619 makes strain be come into full contact with straw powder；It continuously cultivates one month in 28 DEG C of thermostatic chambers, does after fermentation
It is dry, it is spare to obtain fermentation substrate；
（2）The extraction and purifying that extract alcohol rises
The NaOH solution leach steps for being 3.5% with mass fraction（1）Obtained drying for standby fermentation substrate, 60 DEG C of thermostatted waters
It is filtered after bath 1h, stoste is obtained after concentrating filter liquor；Then stoste is adjusted to pH 2.0 with hydrochloric acid, passed through after standing 10 min
4 000rpm centrifuge 15min, and removal precipitation obtains alcohol and rises product after spray-dried；
（3）Alcoholic fermentation
It takes starch 1kg to be put into stainless cylinder of steel, clear water 3L is added, stirring makes starch be uniformly dispersed in water, liquid is then added
The pH of system is adjusted to 6.5 by alpha-amylase 1mL, calcium chloride 1g, feed liquid is heated to 90 DEG C, heat preservation 30min postcoolings are extremely
60 DEG C, regulation system pH to 4.5,2mL liquid saccharifying enzymes are added, 60 DEG C of saccharification 60min is maintained, is cooled to after saccharification
30 DEG C, and activated saccharomyces cerevisiae and alcohol liter 15g is added, ferment 48h in 28 DEG C of thermostatic chambers, obtains fermentation liquid, measures hair
Alcohol concentration in ferment mash is 17% v/v.
2. the method for improving alcoholic fermentation yield according to the Trichoderma Viride extract described in claim 1, it is characterised in that
Straw powder fresh paddy rice stalk crushes after air-drying, and crosses 1mm sieves.
3. the method for improving alcoholic fermentation yield according to the Trichoderma Viride extract described in claim 1, it is characterised in that
The straw powder, water, ammonium nitrate ratio be：Straw powder 1kg, water 3kg, ammonium nitrate 10g；The Trichoderma viride
CGMCC3.6619 inoculum concentrations are the 1% of stalk dry weight.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1451755A (en) * | 2002-04-12 | 2003-10-29 | 中国农业大学 | Method for producing alcohol by solid fermentation of stalks |
| WO2012019308A1 (en) * | 2010-08-12 | 2012-02-16 | Iogen Energy Corporation | Process for co-fermenting glucose and xylose |
| CN103436586A (en) * | 2013-07-18 | 2013-12-11 | 济南开发区星火科学技术研究院 | Process for producing alcohol by utilizing microorganisms to ferment biomass |
| EP2774992A1 (en) * | 2013-03-06 | 2014-09-10 | IFP Energies nouvelles | Process for production of alcohol and / or solvents from biomass with washing of the lignocellulosic solid residue obtained after hydrolysis |
| CN104446895A (en) * | 2014-11-10 | 2015-03-25 | 张常书 | Trichoderma biological humic acid and product thereof |
-
2015
- 2015-04-09 CN CN201510164803.5A patent/CN104988185B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1451755A (en) * | 2002-04-12 | 2003-10-29 | 中国农业大学 | Method for producing alcohol by solid fermentation of stalks |
| WO2012019308A1 (en) * | 2010-08-12 | 2012-02-16 | Iogen Energy Corporation | Process for co-fermenting glucose and xylose |
| EP2774992A1 (en) * | 2013-03-06 | 2014-09-10 | IFP Energies nouvelles | Process for production of alcohol and / or solvents from biomass with washing of the lignocellulosic solid residue obtained after hydrolysis |
| CN103436586A (en) * | 2013-07-18 | 2013-12-11 | 济南开发区星火科学技术研究院 | Process for producing alcohol by utilizing microorganisms to ferment biomass |
| CN104446895A (en) * | 2014-11-10 | 2015-03-25 | 张常书 | Trichoderma biological humic acid and product thereof |
Non-Patent Citations (3)
| Title |
|---|
| 《pretreatment of corn stover using wet oxidation to enhance enzymatic digestibility》;Varga E,et al.;《Applied Biochemistry and Biotechnology》;20030131;第104卷(第1期);37-50 * |
| 《培养基的主要成分对优选酿酒酵母生物量的影响》;王学锋，苑伟，刘延琳;《中国酿造》;20100831(第8期);18-21 * |
| 《腐植酸对酒精酵母刺激作用及用于酿酒提高出酒率的研究》;沈林根等;《江西腐植酸》;19810930(第3期);第34页材料与试验第一段以及第39页讨论第三部分 * |
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| CN104988185A (en) | 2015-10-21 |
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