CN104974958A - Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain - Google Patents
Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain Download PDFInfo
- Publication number
- CN104974958A CN104974958A CN201510397475.3A CN201510397475A CN104974958A CN 104974958 A CN104974958 A CN 104974958A CN 201510397475 A CN201510397475 A CN 201510397475A CN 104974958 A CN104974958 A CN 104974958A
- Authority
- CN
- China
- Prior art keywords
- chromium
- rhizosphere
- culture medium
- red
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a chromium-resisting bacillus separated from rhizosphere of red-spotted stonecrop (pumilus bacillus pumilus JCr9) which is preserved in China General Microbiological Culture Collection Center (CGMCC) on Dec, 1, 2014, with the preservation number of CGMCC No.10078, wherein the 16S rDNA gene sequence is shown in figure 1. The pumilus bacillus pumilus JCr9 is gram-negative bacterium and aerobic corynebacterium, has no atrichia or capsule, has strong tolerance for heavy metal chromium, and has practical significance and important engineering application value for plant-microbial remediation of soil polluted by heavy metal chromium.
Description
Technical field
The present invention relates to bacillus pumilus (Bacillus pumilus) JCr9 with chromium resistance that a strain is separated from red-spotted stonecrop rhizosphere and the application plant-microorganism combine d bioremediation chromium heavy-metal contaminated soil thereof.
Background technology
In recent years, along with industrial expansion, chromium cpd widespread use industrially, the trivalent of generation and hexavalent chromium compound via waste water, waste residue, be discharged into physical environment in a large number containing chromium powder dirt etc., make soil, water body and air be subject to serious pollution of chromium.
The pollution of chromium of China is also very serious, ploughs and is subject to area 2,000 ten thousand hm of heavy metal contamination
2, cause about 20,000,000,000 yuan of financial losses every year.About 600,000 tons of the chromium slag of the annual plant emissions of China according to statistics, over the years accumulative store up chromium slag and reaches 6,000,000 tons, through Detoxified treatment or comprehensive utilization less than 17%.
Pollution of chromium has the features such as potentiality, disguise and chronicity, once polluted-water, soil etc., is also difficult to be recovered, and has very large harm to the health of plant-growth, animal development and human body even if drop into man power and material.Heavy metal in soil is absorbed by plants and enters human body by food chain again, and at people's cylinder accumulation, causes serious threat to the health of the mankind.Cr
6+compound is a kind of carcinogenic substance.Heavy metal chromium can also cause damage to the eyes of people, skin, respiratory tract and stomach.Nearly 3.6 microgram chromium enter human body from respiratory tract for each person every day, account for 1.4% of total intake.The various diseases of the upper respiratory tract may be caused, as perforation of nasal septum, respiratory tract fester, time serious, cause lung cancer etc.Cr
6+there is corrodibility, touch skin and can cause dermatitis, eczema and chrome ulcer etc.
The symbiotic relationship of soil, microorganism, plant can be utilized, give full play to plant and microorganism remediation technology advantage separately, cover the shortage, and then improve the phytoremediation efficiency of pollutant in soil, finally reach the object of thorough restoration of soil polluted by heavy metal.Plant can be microorganism and provides existence place, and transferable oxygen makes the aerobic effect of root circle normally run, root secretions etc. provide a large amount of nutrition for microorganism again, increase the absorption of microbe, also can be used as the degraded that natural Co metabolism substrate promotes pollutent.In addition, microbial process energy pollution abatement thing, to the murder by poisoning of plant, improves tolerance and the semi-invariant of plant.As while utilizing plant to carry out contaminated soil remediation, in soil, inoculation has the obligate degradation bacteria of stronger degradation capability, can promote the degraded of pollutent.
Therefore, be separated the bacterium with stronger chromium tolerance and promotion phytoremediation chromium-polluted soil, to plant-microorganism combine d bioremediation chromium heavy-metal contaminated soil, there is realistic meaning and important engineer applied value.
Summary of the invention
Technical purpose of the present invention is to screen the bacterium that red-spotted stonecrop rhizosphere has stronger chromium tolerance.
Therefore, a first aspect of the present invention relates to chromium resistant strain bacillus pumilus (Bacillus pumilus) JCr9 that a strain is separated from red-spotted stonecrop rhizosphere, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 1st, 2014, and deposit number is CGMCC No.10078.
Preferably, the nucleotide sequence of the 16SrDNA of described red-spotted stonecrop rhizosphere chromium resistant strain Bacillus pumilus JCr9 is as shown in SEQ ID NO:3, sequence not consistent with it in GenBank database, and this bacterial strain 16S rDNA nucleotide sequence does not submit GenBank database to.
A second aspect of the present invention relates to the screening method of above-mentioned red-spotted stonecrop rhizosphere chromium resistant strain Bacilluspumilus JCr9, and it comprises step:
(1) growth selection red-spotted stonecrop plant in order processes: get chromium (Cr) solution mother liquor 2.5ml, and dilution is settled to 40ml, evenly waters in the soil being filled in growth test plant, makes the chromium content of soil reach 1000mgkg
-1; Continue to cultivate 30d under the condition of coercing, to water 40ml at interval of 4d;
(2) separation of bacterial classification, purifying and screening: get 10g soil sample at treated plant rhizosphere, be placed in the bacteria culture medium that 90ml is equipped with granulated glass sphere, 37 DEG C of constant temperature oscillators take off after 150 × g vibration 20-30min, and static 5min, makes soil precipitate; Repeatedly sample at the above every aspect of precipitation, access in new bacteria culture medium, cultivate 24h for 37 DEG C; Get the bacteria suspension after enrichment, coated on the flat board of screening culture medium by multiple dilutions method, be inverted in 37 DEG C of constant incubators, cultivate 48h; Visual inspection, selects the single bacterium colony of typical case of different shape respectively, carries out mark, the separation and picking list bacterium colony is rule in new screening culture medium at the bottom of culture dish; Repeatedly carry out above step, until obtain the consistent pure strain of colony characteristics;
(3) bacterial classification of purifying filtered out is preserved: strain inoculation separation and purification obtained is in bacteria culture medium, 60% (v/v) glycerine is added after cultivation, make the final concentration of glycerine be 10% (v/v), be placed in-80 DEG C and preserve;
Wherein said bacteria culture medium is: extractum carnis 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0;
The compound method of described screening culture medium is as follows: basic medium is bacteria culture medium 1000ml, and add liquid microelement and each 10ml of VITAMIN liquid, 121 DEG C of sterilizing 20min, are cooled to 70 DEG C, add chromium solution mother liquor, make chromium content in substratum be 1000mgL
-1, 37 DEG C of cultivations;
Wherein liquid microelement is: MnSO
40.01g, ZnSO
40.05g, H
3bO
30.01g, CaCl
20.01g, is settled to 1L, and 4 DEG C keep in Dark Place; VITAMIN liquid is: creatine 0.025g, xitix 0.025g, riboflavin 0.025g, and citric acid 0.02g, is settled to 1L, and 4 DEG C keep in Dark Place; Chromium solution mother liquor is: K
2crO
4be mixed with Cr
6+concentration is 200mgml
-1mother liquor, after filtration sterilization, room temperature preservation.
A third aspect of the present invention relates to the composition containing above-mentioned red-spotted stonecrop rhizosphere chromium resistant strain Bacilluspumilus JCr9.
Preferably, described composition has stronger chromium tolerance.
A fourth aspect of the present invention relates to above-mentioned red-spotted stonecrop rhizosphere chromium resistant strain Bacilluspumilus JCr9 and the purposes of described composition in plant-microorganism combine d bioremediation chromium heavy-metal contaminated soil.
A fifth aspect of the present invention relates to above-mentioned red-spotted stonecrop rhizosphere chromium resistant strain Bacilluspumilus JCr9 and described composition contains the purposes in the pollutent of heavy metal chromium in process.Preferably, described pollutent is contaminated soil.
The chromium resistant strain Bacillus pumilus JCr9 utilizing screening method of the present invention to separate from the red-spotted stonecrop plant rhizosphere soil that chromium is coerced, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 1st, 2014, preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.10078; The Classification And Nomenclature of its suggestion is bacillus Bacillus sp.; It is Gram-negative bacteria, aerobic coryneform bacteria, atrichia, without pod membrane.This bacterial strain heavy metal chromium has stronger patience, has realistic meaning and important engineer applied value to plant-microorganism combine d bioremediation chromium heavy-metal contaminated soil.
As well known to those skilled in the art; on the basis of the above-mentioned red-spotted stonecrop rhizosphere chromium resistant strain Bacillus pumilus JCr9 novel species obtained in the present invention; those skilled in the art can carry out suitable mutagenesis to described bacterium and continue the ability improving its heavy metal tolerance chromium, and the mutant strain based on bacterial classification of the present invention after such mutagenesis also falls into the scope of protection of the invention.Described mutagenesis comprises radiation, chemomorphosis etc.Meanwhile, the above-mentioned bacterial strains of the present invention's acquisition and other plant also may use, to reach the object of best plant-microorganism united repairing heavy metal chromium-polluted soil by those skilled in the art jointly.
Accompanying drawing explanation
The 16S rDNA sequence of Fig. 1: bacterial strain Bacillus pumilus JCr9.
The transmission electron microscope photo of Fig. 2: bacterial strain Bacillus pumilus JCr9.
Fig. 3: bacterial strain Bacillus pumilus JCr9 growth curve chart.
Embodiment
To further illustrate the present invention by following non-limiting example below, as well known to those skilled in the art, without departing from the spirit of the invention, can make many amendments to the present invention, such amendment also falls into scope of the present invention.
Following experimental technique if no special instructions, is ordinary method, and the experiment material used if no special instructions, all can easily obtain from commercial company.
Embodiment
The separation of embodiment 1 red-spotted stonecrop rhizosphere chromium resistant strain Bacillus pumilus JCr9
Materials and methods
1, substratum
Bacteria culture medium: extractum carnis 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0.
Preparation screening culture medium: basic medium is bacteria culture medium 1000ml, add liquid microelement and each 10mL of VITAMIN liquid, 121 DEG C of sterilizing 20min, are cooled to about 70 DEG C, add chromium solution mother liquor, make chromium content in substratum be 1000mgL
-1.37 DEG C of cultivations.
Wherein liquid microelement is: MnSO
40.01g, ZnSO
40.05g, H
3bO
30.01g, CaCl
20.01g, is settled to 1L, and 4 DEG C keep in Dark Place.VITAMIN liquid is: creatine 0.025g, xitix 0.025g, riboflavin 0.025g, and citric acid 0.02g, is settled to 1L, and 4 DEG C keep in Dark Place.Chromium solution mother liquor: K
2crO
4be mixed with Cr
6+concentration is 200mgmL
-1mother liquor, after filtration sterilization, room temperature preservation.
2, sample collecting and strains separation
Red-spotted stonecrop plant in order processes growth selection.Get heavy metal Cr solution mother liquor 2.5mL, dilution is settled to 40mL, evenly waters and is filled in the soil of test plant, make the chromium content of soil reach 1000mgkg
-1.Continue to cultivate 30d under the condition of coercing, to water 40mL at interval of 4d.Get 10g soil sample at treated plant rhizosphere, be placed in the bacteria culture medium that 90mL is equipped with granulated glass sphere, 37 DEG C of constant temperature oscillators take off after 150 × g vibration 20-30min, and static 5min, makes soil precipitate.Repeatedly sample at the above every aspect of precipitation, access in new bacteria culture medium, 37 DEG C of enrichment culture 24h.Get the bacteria suspension after enrichment culture, coated on the flat board of screening culture medium by multiple dilutions method, be inverted in 37 DEG C of constant incubators, cultivate 48h.Visual inspection, selects the single bacterium colony of typical case of different shape respectively, carries out mark, the separation and picking list bacterium colony is rule in new screening culture medium at the bottom of culture dish.Repeatedly carry out above step, until obtain the consistent pure strain of colony characteristics, so far complete the separation of bacterial classification, purifying and screening operation.The bacterial classification of purifying filtered out is preserved: strain inoculation separation and purification obtained, in bacteria culture medium, adds 60% (v/v) glycerine after cultivation, make the final concentration of glycerine be 10% (v/v), be placed in-80 DEG C and preserve.Be separated the bacterium that acquisition one strain heavy metal chromium has stronger patience, called after JCr9.
The strain identification of embodiment 2 bacterial strain JCr9
1, extracting genome DNA
Conveniently technique means mass propgation above-mentioned bacterial strains JCr9, then obtains its genomic dna.
2, the authentication method of the 16S rDNA of bacterial strain JCr9
The structure of the pcr amplification of the 16S rDNA of 2.1 bacterial strain JCr9, order-checking and phylogenetic tree
2.1.1 the pcr amplification of 16S rDNA gene order
Forward primer BSF8/20:5'-AGAGTTTGATCCTGGCTCAG-3'(SEQ ID NO:1) and reverse primer BSR1541/20:5 '-AAGGAGGTGATCCAGCCGCA-3 ' (SEQ IDNO:2) the two ends primer of amplification 16S rDNA gene order selects universal primer:.PCR reaction system is 50 μ L, and reaction conditions is 94 DEG C of sex change 5min; Next carry out 35 circulating reactions: 94 DEG C of sex change 45s, 50 DEG C of annealing 45s, 72 DEG C extend 90s; Then 72 DEG C extend 10min again, finally in 4 DEG C of preservations.
2.1.2 the clone and sequence of amplified production
PCR primer TAKARA company produces
vector Cloning Kit is cloned, and first by 16S rDNA gene fragment purifying, is connected to
on Vector carrier, 5 μ L ligation liquid transformed competence colibacillus cell DH5 α, and identify with bacterium colony PCR.The gene order of recon is measured by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
The qualification result of the 16S rDNA of 2.2 bacterial strain JCr9
2.2.1 the 16S rDNA gene order of bacterial strain JCr9
The 16S rDNA gene order of amplification bacterial strain JCr9, obtains the amplified fragments that length is about 1.5kb.Amplified fragments order-checking after glue recovery, connection, clone, the 16S rDNA recording bacterial strain JCr9 is 1482bp (SEQ ID NO:3, asks for an interview Fig. 1 simultaneously), and this sequence does not submit ncbi database to.
2.2.2 the similarity system design of JCr916S rDNA gene order
By the sequence inputting of JCr916S rDNA in ncbi database, Blast program is used the 16S rDNA gene order in the 16S rDNA of this strain bacterium and database to be compared, the bacterial strain with the 16S rDNA gene order of JCr9 with higher similarity is selected, in table 1 from database.
As can be drawn from Table 1, the similarity of bacterial strain JCr9 and Bacillus pumilus BF17, Bacilluspumilus Jo2, Bacillus pumilus SAFR-032, Bacillus pumilus PR13 and Bacillus sp.CSL09-1 bacterial strain is all higher, all can reach more than 99%, and be all the member that Bacillus belongs to.This illustrates that bacterial strain JCr9 is a new subspecies of Bacillus pumilus.
Table 1 bacterial strain JCr916S rDNA sequence NCBI homology search result
3. bacterial strain Bacillus pumilus JCr9 physiological and biochemical analysis
3.1 morphological specificity
HIT H-7650 model transmission electron microscope is adopted to carry out electron microscopic observation to bacterial strain JCr9.This transmission electron microscope resolving power is 0.2nm, and acceleration voltage is 40kV ~ 120kV, and (continuous amplification mode is × 200 ~ × 600000 to enlargement ratio; Low power pattern is × 50 ~ × 1000).
Fig. 2 is shown in by the transmission electron microscope observing photo of bacterial strain JCr9.Bacterial strain JCr9 is Gram-negative, coryneform bacteria, and atrichia, without pod membrane, diameter about 1 μm-1.2 μm, length about 2 μm, the morphological specificity of its bacterium colony is in table 2.
The morphological specificity of table 2 JCr9 bacterial strain bacterium colony
| Bacterial classification | Shape | Diameter (mm) | Color | Edge | Surface | Viscosity | Transparency |
| JCr9 | Circular | 0.8-1.5 | Faint yellow | Neatly | Smoothly | Not thickness | Translucent |
3.2 physiological and biochemical analysis
Carried out physiological and biochemical analysis to bacterial strain JCr9, its analytical results is in table 3.Glycolysis-analysis of experiments the results are shown in Table 4.
The physiological and biochemical test analysis of table 3 Bacillus pumilus JCr9
Note: "+" positive "-" is negative
The glycolytic analysis of table 4 Bacillus pumilus JCr9
| Glycolytic analysis | Bacillus pumilus JCr9 | Glycolytic analysis | Bacillus pumilus JCr9 |
| Glucose | + | Sucrose | + |
| Lactose | - | Maltose | + |
| Semi-lactosi | + | Fructose | + |
| Wood sugar | - |
Note: "+" represents product acid, and "-" represents does not produce acid.
3.3 Bacillus pumilus JCr9 growth curves
Bacillus pumilus JCr9 inoculation is activated 24h in LB liquid nutrient medium, gets 500 μ L and be inoculated in 100mL LB liquid nutrient medium, triangular flask is placed in 37 DEG C of constant temperature oscillator 150 × g and cultivates.Every 2h sampling once, not connect the LB liquid nutrient medium of bacterium for blank, with the light absorption value of bacterial suspension under spectrophotometric colo method mensuration 600nm wavelength, METHOD FOR CONTINUOUS DETERMINATION 36h.Take incubation time as X-coordinate, OD600 is ordinate zou, draws growth curve of bacteria, sees Fig. 3.
As can be seen from Figure 3, bacterial strain JCr9 is in lag phase in inoculating latter 4 hours, within 4-14 hour, is logarithmic phase, enters plateau after 14 hours.
Red-spotted stonecrop rhizosphere chromium resistant strain or the composition containing red-spotted stonecrop rhizosphere chromium resistant strain are processing the application in the pollutent or plant-microorganism combine d bioremediation chromium heavy-metal contaminated soil containing heavy metal chromium.
Claims (5)
1. a strain red-spotted stonecrop rhizosphere chromium resistant strain, its be bacillus pumilus (
bacillus pumilus)
jCr9, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 1st, 2014, and deposit number is CGMCC No.10078.
2. bacterial strain according to claim 1, is characterized in that the nucleotide sequence of its 16S rDNA is as shown in SEQ ID NO:3.
3. the screening method of red-spotted stonecrop rhizosphere chromium resistant strain according to claim 1 and 2, it comprises step:
(1) growth selection red-spotted stonecrop plant in order processes: get chromium (Cr) solution mother liquor 2.5ml, and dilution is settled to 40ml, evenly waters in the soil being filled in growth test plant, makes the chromium content of soil reach 1000mgkg
-1; Continue to cultivate 30d under the condition of coercing, to water 40ml at interval of 4d;
(2) separation of bacterial classification, purifying and screening: get 10g soil sample at treated plant rhizosphere, be placed in the bacteria culture medium that 90ml is equipped with granulated glass sphere, take off after 20-30min that 37 DEG C of constant temperature oscillators vibrate, static 5min, makes soil precipitate; Repeatedly sample at the above every aspect of precipitation, access in new bacteria culture medium, cultivate 24h for 37 DEG C; Get the bacteria suspension after enrichment, coated on the flat board of screening culture medium by multiple dilutions method, be inverted in 37 DEG C of constant incubators, cultivate 48h; Visual inspection, selects the single bacterium colony of typical case of different shape respectively, carries out mark, the separation and picking list bacterium colony is rule in new screening culture medium at the bottom of culture dish; Repeatedly carry out above step, until obtain the consistent pure strain of colony characteristics;
(3) bacterial classification of purifying filtered out is preserved: strain inoculation separation and purification obtained is in bacteria culture medium, 60% (v/v) glycerine is added after cultivation, make the final concentration of glycerine be 10% (v/v), be placed in-80 DEG C and preserve;
Wherein said bacteria culture medium is: extractum carnis 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0;
The compound method of described screening culture medium is as follows: get described bacteria culture medium 1000ml, and add liquid microelement and each 10ml of VITAMIN liquid, 121 DEG C of sterilizing 20min, are cooled to 70 DEG C, add chromium solution mother liquor, make chromium content in substratum be 1000mgL
-1, 37 DEG C of cultivations;
Wherein said liquid microelement is: MnSO
40.01g, ZnSO
40.05g, H
3bO
30.01g, CaCl
20.01g, is settled to 1L, and 4 DEG C keep in Dark Place; Described VITAMIN liquid is: creatine 0.025g, xitix 0.025g, riboflavin 0.025g, and citric acid 0.02g, is settled to 1L, and 4 DEG C keep in Dark Place; Described chromium solution mother liquor is: K
2crO
4be mixed with Cr
6+concentration is 200mgml
-1mother liquor, after filtration sterilization, room temperature preservation.
4. the composition containing the red-spotted stonecrop rhizosphere chromium resistant strain described in claim 1 or 2.
5. red-spotted stonecrop rhizosphere chromium resistant strain according to claim 1 and 2 or composition according to claim 4 are processing the purposes in the pollutent or plant-microorganism combine d bioremediation chromium heavy-metal contaminated soil containing heavy metal chromium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510397475.3A CN104974958A (en) | 2015-07-08 | 2015-07-08 | Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510397475.3A CN104974958A (en) | 2015-07-08 | 2015-07-08 | Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104974958A true CN104974958A (en) | 2015-10-14 |
Family
ID=54271925
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510397475.3A Pending CN104974958A (en) | 2015-07-08 | 2015-07-08 | Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104974958A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110343645A (en) * | 2019-08-09 | 2019-10-18 | 广东省农业科学院蚕业与农产品加工研究所 | One plant of bacterial strain bacillus pumilus SEM-7 and its application |
| CN110607248A (en) * | 2018-06-16 | 2019-12-24 | 华中农业大学 | Fogerella strain A6 for repairing soil cadmium pollution and application |
-
2015
- 2015-07-08 CN CN201510397475.3A patent/CN104974958A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| CHELLAIAH EDWARD RAJA ET AL.: "Characterization of boron resistant and accumulating bacteria Lysinibacillus fusiformis M1,Bacillus cereus M2,Bacillus cereus M3,Bacillus pumilus M4 isolated from former mining site,Hokkaido,Japan", 《JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH》 * |
| MUHAMMAD FAISAL: "Detoxification of Carcinogenic Cr (VI) by Combined Action of Bacillus pumilus-S4 and Pseudomonas doudoroffii-S5 in Associated with Hydrophytes", 《JOURNAL OF PURE AND APPLIED MICROBIOLOGY》 * |
| MUHAMMAD YASIN ET AL.: "Assessing the Phytotoxicity of Tannery Waste-Contaminated Soil on Zea mays (Lin) Growth", 《POL. J. ENVIRON. STUD.》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110607248A (en) * | 2018-06-16 | 2019-12-24 | 华中农业大学 | Fogerella strain A6 for repairing soil cadmium pollution and application |
| CN110607248B (en) * | 2018-06-16 | 2021-01-01 | 华中农业大学 | Fogerella strain A6 for repairing soil cadmium pollution and application |
| CN110343645A (en) * | 2019-08-09 | 2019-10-18 | 广东省农业科学院蚕业与农产品加工研究所 | One plant of bacterial strain bacillus pumilus SEM-7 and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mohanty et al. | Isolation, identification and screening of manganese solubilizing fungi from low-grade manganese ore deposits | |
| CN104974960A (en) | Sedum rhizosphere lead-resistant strain pseudomonas as well as screening method and application thereof | |
| CN105255782B (en) | There is fiber bacterium and the purposes of reducing power to Cr VI | |
| CN103013859B (en) | Contaminated soil phenanthrene and application thereof in contaminated soil restoration | |
| CN112646753B (en) | Klebsiella aerogenes and application thereof | |
| CN103667155B (en) | One bacillus subtilis Bacillus subtilis 3-2 and application thereof | |
| CN112625981B (en) | Serratia marcescens and application thereof | |
| Ajmal et al. | Bacteria isolated from wastewater irrigated agricultural soils adapt to heavy metal toxicity while maintaining their plant growth promoting traits | |
| CN104974959A (en) | Red-spotted stonecrop root lead-resistant strain Providence bacterium, screening method and application of red-spotted stonecrop root lead-resistant strain Providence bacterium | |
| CN103013850A (en) | Salt-resistant alkali-resistant Cr(VI) reduction microbe and screening method thereof | |
| CN106434470A (en) | Polycyclic aromatic hydrocarbon degrading bacterium and applications thereof | |
| CN102533616A (en) | Ralstonia pickettii highly resistant to cadmium and extraction method and application thereof | |
| CN100475950C (en) | Bacillus megaterium and use thereof | |
| CN104403965B (en) | A kind of roost rose of Sharon pseudomonad of water body tetracycline pollutant of degrading and its application | |
| Xu et al. | Biodiversity of culturable endophytic actinobacteria isolated from high yield Camellia oleifera and their plant growth promotion potential | |
| CN105018375A (en) | Sedum rhizosphere cadmium-resistant bacillus sp. strain, screening method and application thereof | |
| CN106337026A (en) | Hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp., and screening method and applications thereof | |
| CN104974958A (en) | Chromium-resisting strain separated from rhizosphere of red-spotted stonecrop, selecting method, and application of shromium-resisting strain | |
| Chai et al. | Hexavalent chromium reduction by Pannonibacter phragmitetus BB isolated from soil under chromium-containing slag heap | |
| CN103695355B (en) | One pseudomonas Pseudomonas otitidis H3 and application thereof | |
| CN102321548B (en) | Rhizobium sp. T3 and applications thereof in microbial degradation hydrogen sulfide | |
| Li et al. | Diversity of cultivable endophytic bacteria associated with halophytes in Xinjiang of China and their plant beneficial traits | |
| Ren et al. | Screening, characteristics and mechanism of Cd-tolerance Cunninghamella bertholletiae | |
| CN110205272A (en) | The Taiwan pseudomonad of one plant of tetracycline that can degrade and its application | |
| CN113976619B (en) | Screening and application of lead-cadmium pollution resistant bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151014 |