CN104970799A - Gait analysis method of laboratory primate - Google Patents
Gait analysis method of laboratory primate Download PDFInfo
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- CN104970799A CN104970799A CN201510365961.7A CN201510365961A CN104970799A CN 104970799 A CN104970799 A CN 104970799A CN 201510365961 A CN201510365961 A CN 201510365961A CN 104970799 A CN104970799 A CN 104970799A
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- gait analysis
- primate
- laboratory animal
- transparent cabinet
- treadmill
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/103—Detecting, measuring or recording devices for testing the shape, pattern, colour, size or movement of the body or parts thereof, for diagnostic purposes
- A61B5/11—Measuring movement of the entire body or parts thereof, e.g. head or hand tremor, mobility of a limb
- A61B5/112—Gait analysis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6887—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient mounted on external non-worn devices, e.g. non-medical devices
- A61B5/6895—Sport equipment
Abstract
The invention discloses a gait analysis method of a laboratory primate. The method comprises the steps that a circle of hair on ankle joints or knee joints or shoulder joints or hip joints on two arms and two legs of the laboratory primate is shaved off, and infrared light reflecting coating is smeared; the laboratory primate smeared with the infrared light reflecting coating is put into a transparent box of a gait analysis device, and the transparent box is controlled by three or more infrared cameras in a surrounded mode; space illumination is adjusted to a gray state, a running machine is started, the laboratory primate walks on a foot path of the running machine, the infrared cameras are connected to a master control computer, gait analysis software is used for monitoring, recording and analyzing, and a result is obtained. The laboratory primate has good compliance, operation is easy, cost is low, captured images are clearer, the test result is objective and real, and the problems that the behavior of the laboratory primate is hard to control, captured images are not clear, and motion tracks are hard to capture can be effectively solved.
Description
Technical field
The present invention relates to motor function and rehabilitation field tests, more particularly relate to the gait analysis method of primate laboratory animal.
Background technology
Gait refers in motor process, limbs a kind of rapport over time and space of pedestrian, and be the regular repetitive sequence of lower limb and mode that move, it is an important parameter of kinesiology and behavior analysis.The concept of biomechanics, method, means and known anatomy, physiological knowledge is utilized, by the image technique of the present computer technology and figure, to the research method that the functional status of walking is analyzed during gait analysis.
Gait analysis range of application is comparatively extensive, after the nerve injury of patient or motor system damage, can be undertaken repairing by multiple means or compensatory, but healing effect how, often needs to be evaluated by the gait analysis of patient.Gait analysis main purpose is to provide detailed appreciation information, to put the crux of the neuromuscular skeletal system of experimenter own in order, via these assessment results, draft best treatment plan, to plan operation, the mode of rehabilitation or wearing accessory treats patient.In addition, the gait analysis after treatment also can assist to confirm that therapeutic effect is to propose further improving countermeasure.For orthopaedics therapy, gait analysis can as the detecting before and after orthopaedic disease treatment and assessment; For recovery treating, gait analysis can as treatment diagnosis, the reference weighed of assessing and return to form.
Primate and human gene's homology high, immunoreation is similar to people, in all animals, physiological and biochemical index and the mankind are the most similar, gait analysis are applied to primate trial animal, for the effect of evaluation medicine, therapeutic scheme, rehabilitation training provides good approach.The current world and domesticly have the more gait analysis method about people or other meiofaunas, but be not suitable for non-human primates laboratory animal, because, the more difficult control of non-human primates laboratory animal behavior, thus cause there is no good gait analysis method.
Summary of the invention
The present invention is to solve the deficiencies in the prior art, provides a kind of gait analysis method being applicable to evaluate primate laboratory animal.The method can solve the more difficult control of primate laboratory animal behavior effectively, obtain not fogging clear, the problem such as movement locus defies capture.
A gait analysis method for primate laboratory animal, comprises the following steps:
1. the ankle joint on two arms and two lower limbs of primate laboratory animal, knee joint, shoulder joint and hip joint place, shave off the hair of this position one circle, and coat infrared reflecting coating, described infrared reflecting coating is the ultrared coating that energy reflective infrared video camera is launched, as monoazo pigment class infrared reflection pigment etc.;
2. will scribble the primate laboratory animal of infrared reflecting coating, put into the transparent cabinet of gait analysis device, with more than 3 thermal cameras around monitoring transparent cabinet;
3. space illumination is adjusted to dim condition, start treadmill, primate laboratory animal is walked on treadmill pavement, and thermal camera is connected to main control computer, is undertaken monitoring, records, is analyzed, to obtain final product by gait analysis software.Described dim condition, refer to Illumination adjusting to the intensity not affecting thermal camera normal acquisition infrared external reflection light and primate laboratory animal and normally walk on a treadmill state, free space illuminator lamp such as daylight lamp adjusts, and increases adjustable resistor on circuit of light sources.
The gait analysis method of the above primate laboratory animal, described gait analysis device comprises treadmill and transparent cabinet, described transparent cabinet is the rectangular box of bottom surface opening, sliding door moving up and down is provided with on rear side of casing, lower end, described transparent cabinet both sides is provided with 4 support components, transparent cabinet and support component is fixed on above treadmill pavement with U-shaped button.Adopt the setting of treadmill pavement, can reduce the length of pavement, device can not only be made easier, it is convenient, accurate to make to record.The setting of sliding door, is convenient to laboratory animal to be positioned in casing.Described treadmill is that the treadmill on open market all can be used.
The gait analysis method of the above primate laboratory animal, the material of described transparent cabinet can be any transparent material with certain degree of hardness, as: transparent plastic, clear glass etc., preferred acrylic material.
Operation principle of the present invention is: primate laboratory animal is put into transparent cabinet, start treadmill, laboratory animal can be walked on the pavement of transparent cabinet, thermal camera launches infrared ray to laboratory animal infrared reflecting coating with it, infrared reflecting coating reflected infrared ray is detected by thermal camera, and information is imported in the gait analysis software of main control computer, and software can connect infrared reflecting point, composition reflective spot group, analyzes the gait of laboratory animal.
The invention has the beneficial effects as follows:
(1) gait analysis method is applied to non-human primates laboratory animal by the present invention, is the gait analysis method closest to the mankind, and the method provides good model vehicle for the screening, pharmacodynamic evaluation etc. of the pathogenetic research of the mankind, medicine.
(2) in order to avoid primate laboratory animal to wear, lighting fitting produces grabs, the resistance action such as to scratch, affect the verity of experimental result, the present invention's application infrared reflecting coating, on the arm being coated in animal and lower limb, the compliance of primate laboratory animal is good, easy to operate, cost is low, it is more clear to catch image, result of the test objective reality.
(3) gait analysis device of the present invention, the infrared light send thermal camera and infrared reflecting coating do not disturb, can by primate laboratory animal restriction on a treadmill, primate laboratory animal compliance is good, effectively solves primate laboratory animal behavior more difficult control, obtains not fogging clear, the problem such as movement locus defies capture.
Accompanying drawing explanation
Fig. 1 is the structural representation of gait analysis device of the present invention
1. treadmill, 2. transparent cabinet, 3. sliding door, 4. support component, 5.U type is buckled, 6. treadmill pavement.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but do not limit the scope of the invention and range of application.
one, main experimental material
Infrared reflection pigment, monoazo pigment class infrared reflection pigment, brand: K-LMAXS, Shanghai Wei Ruiju material trade Co., Ltd provides.
two, the making of gait analysis device
Gait analysis device of the present invention, comprise treadmill (1) and transparent cabinet (2), the rectangular box that described transparent cabinet (2) is bottom surface opening, sliding door moving up and down (3) is provided with on rear side of casing, described transparent cabinet (2) lower end, both sides is provided with 4 support components (4), described transparent cabinet (2) is integral with the adhesion of 4 support components (4), support component (4) is placed in above treadmill side plate, and compressed by U-shaped button (5), make transparent cabinet (2) be fixed on treadmill pavement (6) top.The material of described transparent cabinet (2) and sliding door (3) is acrylic material, and the thickness of material is 1cm.
three, experimental animal
The primate laboratory animal that this test uses cultivates Breeding Center by the male gloomy primates in Guangxi and provides.Through the quarantine of routine and health check-up, damaged without limbs, animal normal behaviour habit is complete, and audit report comprises clinical examination and lab testing content, all performs with reference to national standard.This research is at Guangxi Nanning Lingkang Senoke Biology Science and Technology Co., Ltd AAALAC(Association forAssessment and Accreditation of Laboratory Animal) international qualification certification laboratory completes.
Choose 6 machins, age 10-14 year, wherein 3 female monkeys, 3 public monkeys, and they are divided at random blank group, matched group, experimental group 3 groups, often organize each 2.
four, the gait analysis method of primate laboratory animal
1. blank group.
Get above-mentioned blank group machin 1, put into the transparent cabinet of the above-mentioned gait analysis device made, with 3 thermal cameras around monitoring transparent cabinet; Space illumination is adjusted to dim condition (by LED daylight lamp, circuit increases adjustable resistor, by Illumination adjusting to the intensity not affecting thermal camera normal acquisition infrared external reflection light and machin and can normally walk on a treadmill), start treadmill, governing speed is 10 kilometers/hour, and machin walks 25 minutes on treadmill pavement, and thermal camera is connected to main control computer, undertaken monitoring by gait analysis software, record, analyzed, to obtain final product.
Another 1 machin of blank group operates with method, and machin walks 25 minutes on treadmill pavement.
Result shows: continual walking 25 minutes in the scope that machin can be fixed at gait analysis device, compliance is good.Launch with it Infrared irradiation to machin by thermal camera itself, because monkey limbs joint place does not have strong reflective spot, namely there is not strong reflection particular point by the identification of gait analysis software, therefore cannot carry out gait analysis.
2. matched group.
Get above-mentioned matched group machin 1, on two arms and two lower limbs of machin, choose ankle joint, knee joint, shoulder joint and hip region, bind infrared reflecting bead respectively; To the machin of infrared reflecting bead be tied with, put into the transparent cabinet of the above-mentioned gait analysis device made, with 3 thermal cameras around monitoring transparent cabinet; Space illumination is adjusted to dim condition (intensity of illumination with blank group), start treadmill, governing speed is 10 kilometers/hour, machin walks 14 minutes on treadmill pavement, thermal camera is connected to main control computer, is undertaken monitoring by gait analysis software, record, analyzed, to obtain final product.
Another 1 machin of matched group operates with method, and machin walks 12 minutes on treadmill pavement.
Result shows: machin walks less than 15 minutes in the scope that gait analysis device is fixed, and all grab in the process of walking, the resistance action such as to scratch, have obvious anxiety, aversion, compliance is bad, and the infrared reflecting bead of bondage has the situation of being grabbed or misplacing.Infrared irradiation is launched on the infrared reflecting bead of machin by thermal camera itself, the infrared light that infrared reflecting bead reflects is caught by thermal camera, import signal into computer, and through gait analysis software identification processed, the image reflective spot obtained is clear, but all grabs in walking process, the resistance action such as to scratch, and can not adhere to walking 25 minutes, the infrared reflecting bead of another bondage has by the situation of grabbing, and affects the analysis of result of the test.
3. experimental group.
Get above-mentioned experimental group machin 1, on two arms and two lower limbs of machin, choose ankle joint, knee joint, shoulder joint and hip region, shave off the hair of this position one circle, and coat infrared reflection pigment; To the machin of infrared reflection pigment be scribbled, put into the transparent cabinet of the above-mentioned gait analysis device made, with 3 thermal cameras around monitoring transparent cabinet; Space illumination is adjusted to dim condition (intensity of illumination with blank group), start treadmill, governing speed is 10 kilometers/hour, machin walks 25 minutes on treadmill pavement, thermal camera is connected to main control computer, is undertaken monitoring by gait analysis software, record, analyzed, to obtain final product.
Another 1 machin of experimental group operates with method, and machin walks 25 minutes on treadmill pavement.
Result shows: all continually can adhere to walking 25 minutes in the scope that machin can be fixed at gait analysis device, do not have malaise symptoms in walking process, compliance is good.Launch with it Infrared irradiation to machin by thermal camera itself, because joint scribbles a circle infrared reflecting material, therefore this part to the reflex strength of infrared light far above other position, the infrared light reflected is caught by thermal camera, import signal into computer, and through gait analysis software identification processed, draw 12 bright spots (bright spot is exactly the position scribbling infrared reflecting material) be perfectly clear, software can connect these points of each frame of video, and then draw the dynamic video of these points, gait analysis can be carried out smoothly.
Claims (3)
1. a gait analysis method for primate laboratory animal, is characterized in that, comprises the following steps:
(1) ankle joint on two arms and two lower limbs of primate laboratory animal, knee joint, shoulder joint and hip joint place, shave off the hair of this position one circle, and coat infrared reflecting coating;
To the primate laboratory animal of infrared reflecting coating be scribbled, put into the transparent cabinet of gait analysis device, with more than 3 thermal cameras around monitoring transparent cabinet;
Space illumination is adjusted to dim condition, and start treadmill, primate laboratory animal is walked on treadmill pavement, and thermal camera is connected to main control computer, is undertaken monitoring, records, is analyzed, to obtain final product by gait analysis software.
2. the gait analysis method of primate laboratory animal according to claim 1, it is characterized in that: described gait analysis device comprises treadmill (1) and transparent cabinet (2), the rectangular box that described transparent cabinet (2) is bottom surface opening, sliding door moving up and down (3) is provided with on rear side of casing, described transparent cabinet (2) lower end, both sides is provided with 4 support components (4), with U-shaped button (5), transparent cabinet (2) and support component (4) is fixed on treadmill pavement (6) top.
3. according to the gait analysis method of described primate laboratory animal arbitrary in claim 1 to 2, it is characterized in that: the material of described transparent cabinet is acrylic material.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110132241A (en) * | 2019-05-31 | 2019-08-16 | 吉林化工学院 | A kind of high-precision gait recognition method and device based on time series analysis |
| CN112136706A (en) * | 2020-09-11 | 2020-12-29 | 北京希诺谷生物科技有限公司 | Testing device and method for evaluating dog athletic ability |
| CN113016715A (en) * | 2021-03-23 | 2021-06-25 | 广东省科学院动物研究所 | Method and system for analyzing fine action behaviors of primates |
| CN115116143A (en) * | 2022-08-30 | 2022-09-27 | 中国科学院自动化研究所 | Motion capture method, device, system, electronic device and storage medium |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110132241A (en) * | 2019-05-31 | 2019-08-16 | 吉林化工学院 | A kind of high-precision gait recognition method and device based on time series analysis |
| CN112136706A (en) * | 2020-09-11 | 2020-12-29 | 北京希诺谷生物科技有限公司 | Testing device and method for evaluating dog athletic ability |
| CN113016715A (en) * | 2021-03-23 | 2021-06-25 | 广东省科学院动物研究所 | Method and system for analyzing fine action behaviors of primates |
| CN115116143A (en) * | 2022-08-30 | 2022-09-27 | 中国科学院自动化研究所 | Motion capture method, device, system, electronic device and storage medium |
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Application publication date: 20151014 |