CN104965014B - The preparation method of the compound carbon paste electrode of quantum dot/enzyme for detecting SAM - Google Patents
The preparation method of the compound carbon paste electrode of quantum dot/enzyme for detecting SAM Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of the compound carbon paste electrode of quantum dot/enzyme for detecting SAM, it is characterized in that：Adulterate water solubility CdZnTe quantum dots and transmethylase in carbon paste electrode, and using the methylimidazole hexafluorophosphate of 1 ethyl 3 as adhesive, the carbon paste electrode that CNT and graphite powder are mixed with obtains final product the compound carbon paste electrode of quantum dot/enzyme.Improve 1 ~ 2 times than common carbon paste electrode electric conductivity, electrochemical window is wide, preparation method is simple, low cost, the advantages of surface easily updates, aftercurrent is small；With SAM in the electrode quick detection sample, the method sensitivity is high, the selectivity good, response time is short, interference is few, better than other detection methods, it is a kind of simple and quick, convenient-to-running SAM assay methods, fixed transmethylase electrode sensor low cost, preparation process is simple prepared by the application, specificity is good, with the possibility for realizing automation on-site measurement.
Description
Technical field
It is more particularly to a kind of for detecting S- adenosine first sulphur ammonia the present invention relates to a kind of preparation method of electrochemical sensor
Acid（SAM）The compound carbon paste electrode of quantum dot/enzyme preparation method and application.
Background technology
S-adenosylmethionine（SAM）, SAM contains active methyl, intracellular nearly all first for the modification that methylates
Base both is from SAM first sulphur energy-rich bonds.Due to the popularity of methylation reaction, it may be said that SAM is the important of intracellular participation reaction
Property be only second to a kind of coenzyme of ATP, the minor alteration of intracellular SAM concentration will be produced to the growth of cell, differentiation and function
Significant impact.SAM is mainly by SAM synzyme in bacterial body（MetK）Synthesized by methionine (Met) and ATP.WhenE.coliSAM synthesis enzyme levels decline, when causing intracellular methyl donor SAM to lack, cell would not proper splitting.If
AdoMet hydrolase genes from T3 bacteriophages are importedE.coliSomatic cells, when declining intracellular SAM levels, large intestine angstrom
Uncommon Salmonella also form the thread thalline of nondividing length.Further study showed that, in thread thalline, triggerE.coliCell division
The assembling of Z ring complexs can normally starting, but will not complete, and the SAM synthesis enzyme levels for working as leucine regulation recover normally,
When intracellular methyl donor SAM no longer lacks, cell division also recovers normal immediately.It is obvious that the growth division of bacterial cell
It is closely related with intracellular SAM concentration.
By after methyl transferase catalytic demethyl, the universal product of generation is S- adenosines half Guang high to universal bases donor SAM
Propylhomoserin（SAH）, SAH is found have universal feedback inhibition to the methylation procedure of intracellular protein and nucleic acid, is to turn
The effective competition inhibitor of methylation reaction.In mammalian cell, SAH passes through SAH hydrolases（SAHH）Catalyzing hydrolysis
Generation adenylate and homocysteine, and in the intracellular of most of pathogenic microorganisms, the metabolism of SAH is then using entirely different
Mode --- by adenosylhomocysteine nucleosidase（SAHN）Catalytic pyrolysis generates adenine and S- ribosyls half Guang ammonia high
Acid, SRH further generates homocysteine and 4,5 dihydroxy -2,3- in the presence of S- Ribosylhomocysteinases (SRHH)
Pentanedione（DPD）, homocysteine is finally by several methionine synthetases（MetH、MetE）Regenerate SAM's
Precursor --- methionine, or generate cysteine by multistep enzymatic.
Due to the high specificity of enzyme, the method has that selectivity high, good stability, detection speed be fast, the good, spirit of selectivity
The features such as sensitivity is high.Enzyme electrode research is started in the sixties in 20th century, and since two thousand, biosensor technology is examined in environment
The application of the aspects such as survey, food security, military affairs and medical science is increasingly extensive, public in the patent of Application No. 201410210210.3
The common fixed enzyme electrode preparation method and application of detection hydroquinones and catechol is opened；It is in Authorization Notice No.
A kind of method that the preparation and quick detection peroxide value of vegetable oil of enzyme electrode are disclosed in the patent of CN102435650 B；
Authorization Notice No. in the patent of CN102495115 B to disclose using malic acid in biologic enzyme electrode method detection root exudates
Electrochemical method.
Carbon paste electrode is that, using the Carbon Materials of electric conductivity, such as graphite powder is mixed and made into pastel with the adhesive of hydrophobicity,
Then it is coated in the surface of electrode bar or is packed into the class electrode being made in electrode tube.Because carbon paste electrode is nontoxic, electricity
Chemical window is wide, preparation method is simple, low cost, the advantages of surface easily updates, aftercurrent is small, be widely used to electrochemistry
In analysis, biology sensor preparation and environment measuring, food and medicine analysis.It is such as conductive but carbon paste electrode there is also some shortcomings
Poor performance, sensitivity is low, stability difference etc..In order to improve the performance of carbon paste electrode, prepare to conduct electricity very well in carbon paste electrode
CNT replace graphite powder, and the excellent water-soluble quantum dot of electric conductivity is added in Carbon Materials, quantum dot is also known as partly leading
Body is nanocrystalline, is a kind of nanoparticle being made up of II-VI race or III-group Ⅴ element.Quantum dot due to with different from block
The special light of body material, electrical properties, make it have in the fields such as solar cell, biomarker, fluorescence probe huge
Application value.The quantum dot of synthesis in water is because with good hydrophily and biocompatibility, dissolubility quantum dot is obtained extensively
Application, water-soluble quantum dot is mixed in carbon paste electrode to improve the electric conductivity of electrode.The application is using ionic
Adhesive（Such as ionic liquid）, these attempt all changing to a certain extent the performance of electrode.CNT has excellent
Electron transmission, shows excellent performance in terms of bio-sensing and catalysis.CNT also has self lubricity and biology
Compatibility, so as to be widely used in analytical chemistry field.Ionic liquid is that have to be at room temperature completely from molecular
The salt of liquid, has the advantages that stable in properties, electric conductivity is excellent and electrochemical window is wide, be widely used in material, synthesis,
The field such as living things catalysis and separating and extracting.Ionic liquid accelerates electron transfer rate, improves electrode sensitivity and selectivity.
At present, the method for the measure SAM for having reported has HPLC, and the method has chromatographic column and easily pollutes, and analysis price is held high
The detection method of the first class transferase active that the research S-adenosylmethionine such as expensive defect, AAS, paddy sturdy pines is relied on
(paddy sturdy pines etc., a kind of detection method of the first class transferase active that S-adenosylmethionine is relied on, SCI,
2012,33（3）：521 ~ 525), the method is that dependence transmethylase, adenosylhomocysteine nucleosidase and S- ribosyls are high
SAM is decomposed into homocysteine by the catalytic action of cysteine enzyme, then to homocysteine chromogenic reaction, is operated more numerous
Trivial, the degree of accuracy is also undesirable.Because the matrix of sample is more complicated, difficulty is brought to detection.Therefore, set up it is a kind of it is sensitive,
Quickly, the detection method that easy, high, the reproducible economy of specificity is used, to researcher, manufacturing enterprise, Quality Control personnel, enters
Outlet commodity inspection, government administration section etc. in the urgent need to, to the SAM contents in food, medicine, Environmental security, biological sample
Accurate quantitative analysis determine very necessary, also have for SAM productions and pharmacological research and are of great significance.
Biologic enzyme electrode sensor is that current exploitation has selectivity, stability, detection speed fast, selective good, sensitive
The features such as spending high, is widely used in medical clinic, food, environment and biology sample detection field, and transmethylase modification is existed
The detection for being used for SAM on carbon paste electrode has no report.
The content of the invention
The purpose of the present invention is that transmethylase and water solubility CdZnTe quantum dots are embedded in carbon paste electrode, using 1-
Ethyl-3-methylimidazole hexafluoro woods hydrochlorate prepares a kind of carbon paste electrode and is combined with electrochemistry as adhesive, there is provided quantum
In the preparation method of the compound carbon paste electrode of point/enzyme, and application detection SAM.
Instrument and reagent
CHI660B electrochemical workstations（Shanghai Chen Hua instrument company）, test and use three-electrode system：Supplemented by platinum electrode
Electrode is helped, Ag/AgCl is reference electrode（SCE）, quantum dot/enzyme is combined carbon paste electrode（GCE）It is working electrode；KQ-250E types
Ultrasonic cleaner（Kun Feng ultrasonic instruments Co., Ltd）.
Graphite powder, CNT, tellurium powder, caddy, zinc chloride, sodium borohydride, cysteine, 1- ethyl -3- methyl miaows
Azoles hexafluorophosphate, NaOH, transmethylase（E.C.2.1.1.3）, DNA, SAM；Sulfuric acid, nitric acid, hydrogen peroxide, phosphate
Cushioning liquid, agents useful for same is analyzes pure, and water is deionized water.
The purpose of the present invention is achieved through the following technical solutions.
The preparation method of the compound carbon paste electrode of a kind of quantum dot/enzyme, it is characterised in that there is the method following technique to walk
Suddenly：
（1）It is prepared by semiconductor precursor solution：In the reactor, by tellurium powder and the mol ratio 1 of sodium borohydride：2 ~ 4 addition
During quality is 25 ~ 50 times of deionized waters, under argon gas protection, the min of ultrasonic disperse 10, in 60 DEG C of constant temperature back flow reactions reaction 4 ~
6h, is cooled to room temperature, is placed in ice-water bath, obtains semiconductor precursor solution；
（2）It is prepared by zinc cadmium precursor liquid：In the reactor, caddy is added by following mass percent：0.1 ~ 1.2%, chlorination
Zinc：0.1 ~ 1.0%, cysteine：0.2 ~ 1.5%, deionized water：97 ~ 99%, each component sum is a hundred per cent, uses NaOH
Regulation pH value of solution is 10, is passed through argon gas, in 80 ~ 90 DEG C of constant temperature 3 ~ 5h of back flow reaction, is cooled to room temperature, and zinc cadmium precursor liquid is obtained；
（3）The preparation of water-soluble CdZnTe quantum dots：By step（1）The semiconductor precursor solution of preparation is under argon gas protection
Add step（2）In the zinc cadmium precursor liquid of preparation, in 85 DEG C of constant temperature 4 ~ 6h of back flow reaction, room temperature is cooled to, water solubility is obtained
CdZnTe quantum dots；
（4）CNT and graphite powder are pre-processed：In the reactor, graphite powder is added by following mass percent：20~
30%, CNT：8 ~ 15%, the concentrated sulfuric acid：40 ~ 50%, concentrated nitric acid：10 ~ 20%, each component sum is a hundred per cent, in 80 ~ 90 DEG C
3 ~ 5h of isothermal reaction, is cooled to room temperature, filtering, and it is neutrality to be washed with deionized, and is dried in thermostatic drying chamber, is ground to powder
End, obtains final product pretreatment CNT and graphite powder mixture；
（5）The preparation of the compound carbon paste electrode of quantum dot/enzyme：By water-soluble CdZnTe quantum dots：Transmethylase：1- second
Base -3- methylimidazole hexafluorophosphates：CNT and graphite powder mixture are 1 in mass ratio：0.2~0.4：4~10：80~90
It is well mixed, grind uniform in agate mortar, dried in 60 DEG C of warm drying boxes, obtain final product water-soluble CdZnTe quantum dots/first
Based transferase/1- ethyl-3-methylimidazoles hexafluorophosphate/CNT and graphite powder mixture carbon paste；Then by its carbon paste
Loading is connected with the interior glass tube through being Φ 4mm of copper cash, and compacting is polished with abrasive paper for metallograph, polishing, deionized water washing, i.e.,
Obtain the compound carbon paste electrode of quantum dot/enzyme.
It is as follows that the compound carbon paste electrode sensor of quantum dot/enzyme determines SAM steps：
（1）Standard liquid is prepared：Prepare one group of SAM standard liquid including the various concentrations including blank standard specimen, bottom liquid
It is the PBS of pH7.0 ~ 8.0；
（2）It is reference electrode by Ag/AgCl, platinum electrode is auxiliary electrode, quantum dot/enzyme composite carbon prepared by the present invention
Paste electrode is that working electrode constitutes three-electrode system, connects CHI660B electrochemical workstations, and bottom liquid delays for the phosphate of pH7.0
Solution is rushed, in the potential range of -1.5 ~ 0.2V, with 50mV/s scan round 15min, washing is taken out.Then chrono-amperometric is used
Method scans the solution, and operating voltage is -1.1V, takes the peak point current of SAM and SAM concentration under various concentrations and works curve；
（3）The detection of SAM：Replace step with testing sample（1）In SAM standard liquids, according to step（2）Method enter
Row detection, according to the difference of response current reduction△IAnd working curve, obtain the content of SAM in testing sample.
Advantages of the present invention and effect are：
（1）The present invention prepares the compound carbon paste electrode of quantum dot/enzyme, and adulterate water solubility CdZnTe quantum in carbon paste electrode
Point, using 1- ethyl-3-methylimidazoles hexafluorophosphate as adhesive, the carbon paste that CNT and graphite powder are mixed with is electric
1 ~ 2 times common of carbon paste electrode electric conductivity raising of ultimate ratio, and the surface modification of electrode is not needed, electrochemical window is wide, system
Preparation Method is simple, low cost, the advantages of surface easily updates, aftercurrent is small；
（2）The compound carbon paste electrode sensor of the quantum dot/enzyme shows very high selectivity and sensitivity, response electricity to SAM
Stream is in good linear relationship in the range of 0.5 ~ 12 μm of ol/L with the concentration of SAM, and coefficient R=0.998, detection is limited to
8.92×10-7mol/L；
（3）The compound carbon paste electrode of the quantum dot/enzyme does not use poisonous reagent, environment-friendly and green during preparation；
（4）Quantum dot/enzyme prepared by the present invention is combined SAM during carbon paste electrode sensor is used successfully to medicine, food
In detection, SAM detection difficults are solved.
Specific embodiment
Embodiment 1
（1）It is prepared by semiconductor precursor solution：In the reactor, it is separately added into the tellurium powder of 1.0g, the sodium borohydride of 0.9g,
In the deionized water of 50mL, under argon gas protection, the min of ultrasonic disperse 10, in 60 DEG C of constant temperature back flow reaction reaction 5h, is cooled to
Room temperature, is placed in ice-water bath, obtains semiconductor precursor solution；
（2）It is prepared by zinc cadmium precursor liquid：In the reactor, it is separately added into caddy：0.6g, zinc chloride：0.5g, half Guang ammonia
Acid：0.9g, water：98mL, it is 10 with sodium hydrate regulator solution pH, argon gas is passed through, in 85 DEG C of constant temperature back flow reaction 4h, cooling
To room temperature, zinc cadmium precursor liquid is obtained；
（3）The preparation of water-soluble CdZnTe quantum dots：By step（1）The semiconductor precursor solution of preparation is under argon gas protection
Add step（2）In the zinc cadmium precursor liquid of preparation, in 85 DEG C of constant temperature back flow reaction 5h, room temperature is cooled to, water solubility is obtained
CdZnTe quantum dots；
（4）CNT and graphite powder are pre-processed：In the reactor, it is separately added into 25g graphite powders, 10g CNTs,
The 25mL concentrated sulfuric acids, 14mL concentrated nitric acids, in 85 DEG C of isothermal reaction 4h, are cooled to room temperature, filtering, and it is neutrality to be washed with deionized,
Dried in thermostatic drying chamber, be ground to powder, obtain final product pretreatment CNT and graphite powder mixture；
（5）The preparation of the compound carbon paste electrode of quantum dot/enzyme：In agate mortar, the transmethylase of 0.2g is separately added into,
The water-soluble CdZnTe quantum dots of 0.8g, the 1- ethyl-3-methylimidazole hexafluorophosphates of 2.0g, 18g CNTs and graphite
Powder mixture, grinds uniform in agate mortar, is dried in 60 DEG C of warm drying boxes, obtains final product CdZnTe quantum dots/methyl transfer
Enzyme/1- ethyl-3-methylimidazoles hexafluorophosphate/CNT and graphite powder mixture carbon paste；Then its carbon paste is loaded and is connected
There is the interior through in the glass tube of Φ 4mm, compacting is polished with abrasive paper for metallograph, polishing, deionized water washing obtains final product quantum of copper cash
Point/enzyme is combined carbon paste electrode.
Embodiment 2
（1）It is prepared by semiconductor precursor solution：In the reactor, it is separately added into the tellurium powder of 0.5g, the sodium borohydride of 0.6g,
In the deionized water of 30mL, under argon gas protection, the min of ultrasonic disperse 10, in 60 DEG C of constant temperature back flow reaction reaction 4h, is cooled to
Room temperature, is placed in ice-water bath, obtains semiconductor precursor solution；
（2）It is prepared by zinc cadmium precursor liquid：In the reactor, it is separately added into caddy：1.0g, zinc chloride：0.8g, half Guang ammonia
Acid：1.2g, water：97mL, it is 10 with sodium hydrate regulator solution pH, argon gas is passed through, in 80 DEG C of constant temperature back flow reaction 5h, cooling
To room temperature, zinc cadmium precursor liquid is obtained；
（3）The preparation of water-soluble CdZnTe quantum dots：By step（1）The semiconductor precursor solution of preparation is under argon gas protection
Add step（2）In the zinc cadmium precursor liquid of preparation, in 85 DEG C of constant temperature back flow reaction 4h, room temperature is cooled to, water solubility is obtained
CdZnTe quantum dots；
（4）CNT and graphite powder are pre-processed：In the reactor, it is separately added into 20g graphite powders, 15g CNTs,
The 27mL concentrated sulfuric acids, 11mL concentrated nitric acids, in 80 DEG C of isothermal reaction 5h, are cooled to room temperature, filtering, and it is neutrality to be washed with deionized,
Dried in thermostatic drying chamber, be ground to powder, obtain final product pretreatment CNT and graphite powder mixture；
（5）The preparation of the compound carbon paste electrode of quantum dot/enzyme：In agate mortar, the transmethylase of 0.1g is separately added into,
The water-soluble CdZnTe quantum dots of 0.5g, the 1- ethyl-3-methylimidazole hexafluorophosphates of 1.2g, 9.5g CNTs and graphite
Powder mixture, grinds uniform in agate mortar, is dried in 60 DEG C of warm drying boxes, obtains final product CdZnTe quantum dots/methyl transfer
Enzyme/1- ethyl-3-methylimidazoles hexafluorophosphate/CNT and graphite powder mixture carbon paste；Then its carbon paste is loaded and is connected
There is the interior through in the glass tube of Φ 4mm, compacting is polished with abrasive paper for metallograph, polishing, deionized water washing obtains final product quantum of copper cash
Point/enzyme is combined carbon paste electrode.
Embodiment 3
（1）It is prepared by semiconductor precursor solution：In the reactor, it is separately added into the tellurium powder of 0.8g, the sodium borohydride of 0.5g,
In the deionized water of 40mL, under argon gas protection, the min of ultrasonic disperse 10, in 60 DEG C of constant temperature back flow reaction reaction 6h, is cooled to
Room temperature, is placed in ice-water bath, obtains semiconductor precursor solution；
（2）It is prepared by zinc cadmium precursor liquid：In the reactor, it is separately added into caddy：0.2g, zinc chloride：0.2g, half Guang ammonia
Acid：0.6g, water：99mL, it is 10 with sodium hydrate regulator solution pH, argon gas is passed through, in 85 DEG C of constant temperature back flow reaction 4h, cooling
To room temperature, zinc cadmium precursor liquid is obtained；
（3）The preparation of water-soluble CdZnTe quantum dots：By step（1）The semiconductor precursor solution of preparation is under argon gas protection
Add step（2）In the zinc cadmium precursor liquid of preparation, in 85 DEG C of constant temperature back flow reaction 6h, room temperature is cooled to, water solubility is obtained
CdZnTe quantum dots；
（4）CNT and graphite powder are pre-processed：In the reactor, it is separately added into 30g graphite powders, 8g CNTs,
The 24mL concentrated sulfuric acids, 13mL concentrated nitric acids, in 90 DEG C of isothermal reaction 4.5h, are cooled to room temperature, filtering, are washed with deionized in
Property, dried in thermostatic drying chamber, powder is ground to, obtain final product pretreatment CNT and graphite powder mixture；
（5）The preparation of the compound carbon paste electrode of quantum dot/enzyme：In agate mortar, the transmethylase of 0.1g is separately added into,
The water-soluble CdZnTe quantum dots of 0.2g, the 1- ethyl-3-methylimidazole hexafluorophosphates of 0.6g, 8.5g CNTs and graphite
Powder mixture, grinds uniform in agate mortar, is dried in 60 DEG C of warm drying boxes, obtains final product CdZnTe quantum dots/methyl transfer
Enzyme/1- ethyl-3-methylimidazoles hexafluorophosphate/CNT and graphite powder mixture carbon paste；Then its carbon paste is loaded and is connected
There is the interior through in the glass tube of Φ 4mm, compacting is polished with abrasive paper for metallograph, polishing, deionized water washing obtains final product quantum of copper cash
Point/enzyme is combined carbon paste electrode.
Embodiment 4
（1）It is prepared by semiconductor precursor solution：In the reactor, it is separately added into the tellurium powder of 0.6g, the sodium borohydride of 0.4g,
In the deionized water of 50mL, under argon gas protection, the min of ultrasonic disperse 10, in 60 DEG C of constant temperature back flow reaction reaction 5.5h, cooling
To room temperature, it is placed in ice-water bath, obtains semiconductor precursor solution；
（2）It is prepared by zinc cadmium precursor liquid：In the reactor, it is separately added into caddy：1.0g, zinc chloride：1.0g, half Guang ammonia
Acid：1.0g, water：97mL, it is 10 with sodium hydrate regulator solution pH, argon gas is passed through, it is cold in 85 DEG C of constant temperature back flow reaction 4.5h
But to room temperature, zinc cadmium precursor liquid is obtained；
（3）The preparation of water-soluble CdZnTe quantum dots：By step（1）The semiconductor precursor solution of preparation is under argon gas protection
Add step（2）In the zinc cadmium precursor liquid of preparation, in 85 DEG C of constant temperature back flow reaction 5.5h, room temperature is cooled to, water solubility is obtained
CdZnTe quantum dots；
（4）CNT and graphite powder are pre-processed：In the reactor, it is separately added into 28g graphite powders, 12g CNTs,
The 26mL concentrated sulfuric acids, 9mL concentrated nitric acids, in 85 DEG C of isothermal reaction 4.5h, are cooled to room temperature, filtering, and it is neutrality to be washed with deionized,
Dried in thermostatic drying chamber, be ground to powder, obtain final product pretreatment CNT and graphite powder mixture；
（5）The preparation of the compound carbon paste electrode of quantum dot/enzyme：In agate mortar, the transmethylase of 0.1g is separately added into,
The water-soluble CdZnTe quantum dots of 0.3g, the 1- ethyl-3-methylimidazole hexafluorophosphates of 0.8g, 9g CNTs and graphite powder
Mixture, grinds uniform in agate mortar, is dried in 60 DEG C of warm drying boxes, obtain final product CdZnTe quantum dots/transmethylase/
1- ethyl-3-methylimidazoles hexafluorophosphate/CNT and graphite powder mixture carbon paste；Then its carbon paste is loaded and is connected with copper
The interior of line is polished through in the glass tube of Φ 4mm, to be compacted, being polished with abrasive paper for metallograph, and deionized water washing obtains final product quantum dot/enzyme
Compound carbon paste electrode.
Embodiment 5
By the compound carbon paste electrode sensor of quantum dot/enzyme prepared by above-described embodiment 1 ~ 4, for the inspection of SAM in medicine
Survey, step is as follows：
（1）Standard liquid is prepared：Prepare one group of SAM standard liquid including the various concentrations including blank standard specimen, bottom liquid
It is the PBS of pH 7.5；
（2）Working curve is drawn：It is reference electrode by Ag/AgCl, platinum electrode is auxiliary electrode, electricity prepared by the present invention
Extremely working electrode composition three-electrode system, connects CHI660B electrochemical workstations, and the solution is scanned using chronoamperometry,
Operating voltage is -1.1V, goes the peak point current of SAM and SAM concentration under various concentrations to work curve, the recurrence of working curve
Equation is I=0.017+0.409c (μm ol/L), and coefficient R=0.998, the range of linearity of detection is 0.5 ~ 12 μm of ol/L, inspection
Rising limit 18.92 × 10-7mol/L；
（3）The detection of SAM：20, Transmetil tablet is taken, after grinding, is soaked with deionized water and gone 1 hour, filtered, filtrate is fixed
Hold in 250 mL volumetric flasks, be diluted in the range of working curve during measure, step is replaced with testing sample（1）In SAM mark
Quasi- solution, according to step（2）Method detected, according to response current value and working curve, obtain SAM in testing sample
Content；The rate of recovery is between 96.21 ~ 104.65%.
Immobilized transmethylase electrode sensor prepared by the present invention is used successfully to SAM in medicine, food, biological sample
In detection, the rate of recovery is between 96.21 ~ 104.65%%, therefore molecular engram sensor prepared by the present invention can be widely applied to
The association areas such as chemical industry, biological medicine, food, environmental protection tests, solve the difficulty of SAM detections.
Claims (3)
1. a kind of quantum dot/enzyme is combined the preparation method of carbon paste electrode, it is characterised in that the method has following processing step：
(1) prepared by semiconductor precursor solution：In the reactor, by tellurium powder and the mol ratio 1 of sodium borohydride：2~4 addition quality
In for 25~50 times of deionized waters, under argon gas protection, ultrasonic disperse 10min reacts 4~6h in 60 DEG C of constant temperature back flow reactions,
Room temperature is cooled to, is placed in ice-water bath, obtain semiconductor precursor solution；
(2) prepared by zinc cadmium precursor liquid：In the reactor, caddy is added by following mass percent：0.1~1.2%, chlorination
Zinc：0.1~1.0%, cysteine：0.2~1.5%, deionized water：97~99%, each component sum is a hundred per cent, uses hydrogen
Sodium oxide molybdena regulation pH value of solution is 10, is passed through argon gas, in 80~90 DEG C of constant temperature 3~5h of back flow reaction, is cooled to room temperature, and zinc cadmium is obtained
Preceding body fluid；
(3) preparation of water solubility CdZnTe quantum dots：Semiconductor precursor solution prepared by step (1) is added under argon gas protection
In zinc cadmium precursor liquid prepared by step (2), in 85 DEG C of constant temperature 4~6h of back flow reaction, room temperature is cooled to, water solubility CdZnTe is obtained
Quantum dot；
(4) CNT and graphite powder are pre-processed：In the reactor, graphite powder is added by following mass percent：20~30%,
CNT：8~15%, the concentrated sulfuric acid：40~50%, concentrated nitric acid：10~20%, each component sum for absolutely, in 80~
90 DEG C of 3~5h of isothermal reaction, are cooled to room temperature, filtering, and it is neutrality to be washed with deionized, and are dried in thermostatic drying chamber, grind
Powder is milled to, pretreatment CNT and graphite powder mixture is obtained final product；
(5) preparation of the compound carbon paste electrode of quantum dot/enzyme：By water-soluble CdZnTe quantum dots：Transmethylase：1- ethyls -3-
Methylimidazole hexafluorophosphate：CNT and graphite powder mixture are 1 in mass ratio：0.2~0.4：4~10：80~90 mix
Close uniform, grind uniform in agate mortar, dried in 60 DEG C of warm drying boxes, obtain final product water-soluble CdZnTe quantum dots/methyl
Transferase/1- ethyl-3-methylimidazoles hexafluorophosphate/CNT and graphite powder mixture carbon paste；Then its carbon paste is filled
Enter to be connected with the internal diameter of copper cash in the glass tube of Φ 4mm, compacting is polished with abrasive paper for metallograph, polishing, deionized water washing is obtained final product
Quantum dot/enzyme is combined carbon paste electrode.
2. a kind of preparation method of the compound carbon paste electrode of quantum dot/enzyme according to claim 1, is characterised by, step (5)
Described transmethylase is E.C.2.1.1.3 type transmethylases.
3. a kind of quantum dot/enzyme according to claim 1 is combined the quantum dot/enzyme prepared by the preparation method of carbon paste electrode
Compound carbon paste electrode sensor, is characterised by, the compound carbon paste electrode sensor of prepared quantum dot/enzyme is used for S- glands in sample
The measure of glycosides methionine.
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| CN1496411A (en) * | 2001-01-12 | 2004-05-12 | ��Ī������ | Method and apparatus for simultaneous quantification of different radionuclides on the surface of biological microarray |
| WO2007032788A2 (en) * | 2005-05-24 | 2007-03-22 | Sandia National Laboratories | Method and apparatus for gel electrophoretic immunoassay |
| CN102879442A (en) * | 2012-10-12 | 2013-01-16 | 上海交通大学 | Graphene quantum dot modified electrochemical sensor, and preparation method and application thereof |
| CN103454325A (en) * | 2013-09-04 | 2013-12-18 | 上海移宇科技有限公司 | Photocatalysed glucose microelectrode sensor and preparation method thereof |
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| CN1496411A (en) * | 2001-01-12 | 2004-05-12 | ��Ī������ | Method and apparatus for simultaneous quantification of different radionuclides on the surface of biological microarray |
| WO2007032788A2 (en) * | 2005-05-24 | 2007-03-22 | Sandia National Laboratories | Method and apparatus for gel electrophoretic immunoassay |
| CN102879442A (en) * | 2012-10-12 | 2013-01-16 | 上海交通大学 | Graphene quantum dot modified electrochemical sensor, and preparation method and application thereof |
| CN103454325A (en) * | 2013-09-04 | 2013-12-18 | 上海移宇科技有限公司 | Photocatalysed glucose microelectrode sensor and preparation method thereof |
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