CN104950026B - Electrochemical luminescence bioanalysis flow cell based on magnetic bead - Google Patents
Electrochemical luminescence bioanalysis flow cell based on magnetic bead Download PDFInfo
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- CN104950026B CN104950026B CN201510345054.6A CN201510345054A CN104950026B CN 104950026 B CN104950026 B CN 104950026B CN 201510345054 A CN201510345054 A CN 201510345054A CN 104950026 B CN104950026 B CN 104950026B
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- 239000011324 bead Substances 0.000 title claims abstract description 44
- 238000011953 bioanalysis Methods 0.000 title claims abstract description 43
- 238000004020 luminiscence type Methods 0.000 title claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 230000003287 optical effect Effects 0.000 claims abstract description 33
- 230000037361 pathway Effects 0.000 claims description 13
- 239000013307 optical fiber Substances 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 239000011505 plaster Substances 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 3
- 241000208340 Araliaceae Species 0.000 claims 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims 1
- 235000003140 Panax quinquefolius Nutrition 0.000 claims 1
- 235000008434 ginseng Nutrition 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 8
- 238000004458 analytical method Methods 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 description 19
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- 239000000243 solution Substances 0.000 description 7
- 238000013461 design Methods 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 238000011010 flushing procedure Methods 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
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- 230000007547 defect Effects 0.000 description 1
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- 239000006210 lotion Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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Abstract
The present invention relates to a kind of electrochemical luminescence bioanalysis flow cell based on magnetic bead, including with the top opening being oppositely arranged and the funnel of bottom opening, funnel bottom is arranged on the seal of the bottom opening of closing funnel, it is arranged on the working electrode on the upper surface of seal, be embedded on funnel to electrode, the lid of the top opening of closing funnel and the magnet that can be moved relative to working electrode, to axial rings of the electrode along funnel around and be embedded in funnel, funnel includes inwall and the funnel chamber for setting and being formed is enclosed by inwall, the optical channel of lid is provided through on lid, optical channel is connected with funnel chamber, optical mirror slip is provided with optical channel, liquid access way is offered on lid, funnel chamber passes through liquid access way and ft connection, the electrochemical luminescence bioanalysis flow cell based on magnetic bead is integrally miniaturized, ECL reaction reagents for bioanalysis are relatively fewer, the ECL reaction reagents consumed are relatively fewer, and without dead volume, liquid Rapid replacement, realize quick analysis.
Description
Technical field
The present invention relates to a kind of electrochemical luminescence bioanalysis flow cell based on magnetic bead, belong to detection device.
Background technology
In in the past few decades, electrochemical luminescence (ECL) bioanalysis research based on magnetic bead achieves huge progress,
Corresponding detecting instrument mainly includes electrochemiluminescence cell, electro-optic detector and detector three parts.Electrochemiluminescence cell
It is the core of electrochemical luminescence research system.The signal acquisition of ECL analyses and detection all rely on the performance in the pond with setting
Meter.But because the electrochemiluminescence cell of current commercialization is still fewer, specialized degree is low and many performances also imperfection, big portion
Divide researcher must be according to respective requirement of experiment come designed, designed and making electrochemiluminescence cell.
At present for electrochemiluminescence cell tectonic sieving, compared with frequently be three-electrode system, i.e., working electrode, to electricity
Pole and reference electrode.Electrochemiluminescence cell can be divided into flow cell and static pond two types according to input mode.Because with it is quiet
State pond is compared, flow cell have the advantages that quickly, reappearance is good, be easy to automate and can monitor on-line, and by extensive
Concern, it, which designs and made, also turns into an important directions in electrochemiluminescence cell research.
Although common electrochemiluminescence cell makes and easy to use, but still the part that comes with some shortcomings：
1. three electrodes will be inserted into pond, the space in pond is occupied, development of the detection architecture to miniaturization is limited, this
More need to consume substantial amounts of ECL reaction reagents when external actually detected；
2. being loaded manually, pond body is dismantled repeatedly causes the relative position of three electrodes to keep fixed, influence ECL detection knots
The reappearance of fruit；
3. the relative area of pair electrode is smaller；
4. pond body is larger dead volume etc..
These will all cause the reduction of electrochemiluminescence cell performance and its analytical precision and sensitivity.In view of above-mentioned
Defect, the design people is actively subject to research and innovation, to found a kind of life of the electrochemical luminescence based on magnetic bead of new structure
Thing analyzes flow cell, makes it with more the value in industry.
The content of the invention
It is miniaturized it is an object of the invention to provide one kind, and the less electricity based on magnetic bead of ECL reaction reagents consumed
Chemiluminescence bioanalysis flow cell.
To achieve these goals, the technical solution adopted in the present invention is as follows：A kind of electrochemical luminescence based on magnetic bead
Bioanalysis flow cell, including with the top opening being oppositely arranged and the funnel of bottom opening, be arranged on the funnel bottom to seal
Close the seal of the bottom opening of funnel, the working electrode being arranged on the upper surface of the seal, be embedded on the funnel
To electrode, the lid of the top opening of the closing funnel and the magnet that can be moved relative to the working electrode, it is described to electrode
Along funnel axial rings around and be embedded in funnel, the funnel includes inwall and the funnel chamber for setting and being formed, the lid is enclosed by inwall
The optical channel of the lid is provided through on body, the optical channel is connected with funnel chamber, and optics is provided with the optical channel
Liquid access way is offered on eyeglass, the lid, the funnel chamber passes through liquid access way and ft connection.
Further, the liquid access way includes feed pathway and liquid outlet channel, and the lid, which has, stretches to institute
The plunger portion in funnel chamber is stated, the feed pathway has the feed liquor opening being opened in the plunger portion, the liquid outlet channel
Go out liquid opening with being opened in the plunger portion, the feed liquor opening goes out liquid opening less than described in.
Further, the feed liquor opening plaster is leaned on to the inwall, and the plunger portion is in circular platform type, it is described go out liquid opening be
Along the spiral slot of the side wall extension of the plunger portion.
Further, the electrochemical luminescence bioanalysis flow cell based on magnetic bead also includes reference electrode, described right
Perforate is offered on electrode, one end of the reference electrode is arranged in the perforate.
Further, the electrochemical luminescence bioanalysis flow cell based on magnetic bead includes middle part cylinder, the funnel
Formed in the middle part cylinder, the fixing groove of the fixed reference electrode is offered on the middle part cylinder.
Further, the electrochemical luminescence bioanalysis flow cell based on magnetic bead includes being located under the middle part cylinder
The bottom cylinder of side, the bottom of the middle part cylinder sets fluted, and the seal is to be protruded out upwards from the bottom cylinder
The boss of formation, the seal coordinates with the groove, and the fluting for housing the magnet, institute are offered on the bottom cylinder
State fluting and be additionally provided with moving member in the bottom surface of the bottom cylinder, the fluting, the magnet is arranged on the movement
On part.
Further, sample holes are offered on the lid, the funnel chamber passes through the sample holes and ft connection, institute
State and sealing-plug is provided with sample holes.
Further, the sample holes are formed from bifurcated in the feed pathway.
Further, the electrochemical luminescence flow cell also includes the optical fiber being arranged in the optical channel.
Further, the optical channel is through in the bottom wall of lid, the optical channel from the side wall of the lid and had
Reflective mirror is provided with corner, the corner.
By such scheme, the present invention at least has advantages below：The electrochemical luminescence based on magnetic bead of the present invention is biological
Then analyze flow cell can pass through work by magnet by the antigen antibody complex absorption with magnetic bead on the working electrode (s
Electrode, the detection to electrode and optical channel realization to antigen antibody complex, compared with prior art, should the electrification based on magnetic bead
Learn luminous organism analysis flow cell to be integrally miniaturized, funnel is shown in electrochemical luminescence bioanalysis flow cell self structure
The effective volume of chamber is smaller, in addition, compared with prior art, the ECL reaction reagents for bioanalysis are relatively fewer, that is, disappeared
The ECL reaction reagents of consumption are relatively fewer.
Further：Because the feed liquor opening of feed pathway less than liquid outlet channel goes out liquid opening, to keep unidirectional stream, punching
Wash clean, it is to avoid the result of influence detection, what is more, because feed liquor opening plaster is leaned on to inwall, it is along plunger portion to go out liquid opening
The spiral slot of side wall extension, and plunger portion design, so as to be more conducive to avoid rinsing dead angle, be more conducive to avoid influence from examining
The result of survey.
In addition, by setting sample holes on lid so that detection sample Rapid replacement, and also contribute to improve base
In the service life of the electrochemical luminescence bioanalysis flow cell of magnetic bead.Simultaneously as the bottom opening of sample holes is opened for feed liquor
Mouthful, and the feed liquor opening plaster is leaned on to inwall, and hopper design is used, so that when inserting sample, sample can be distributed to along inwall
On working electrode, detection speed is favorably improved, it helps improve detection sensitivity, realize quick analysis.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate accompanying drawing describe in detail as after.
Brief description of the drawings
Fig. 1 is the structural representation of the electrochemical luminescence bioanalysis flow cell based on magnetic bead shown in one embodiment of the invention
Figure；
Fig. 2 is the structural representation of lid in Fig. 1；
Fig. 3 is the sectional view of the electrochemical luminescence bioanalysis flow cell based on magnetic bead shown in Fig. 1；
Fig. 4 is the electrochemical luminescence bioanalysis flow cell based on magnetic bead shown in Fig. 1 in the sectional view on another visual angle；
Fig. 5 sample injection times investigate figure；
Fig. 6 washing times investigate figure；
Fig. 7 is the section view of the electrochemical luminescence bioanalysis flow cell based on magnetic bead shown in another embodiment of the present invention
Figure.
Embodiment
With reference to the accompanying drawings and examples, the embodiment to the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
Referring to Fig. 1 to Fig. 4, a kind of electrochemical luminescence bioanalysis circulation based on magnetic bead described in the embodiment of the present invention one
Pond 100 includes with the top opening (non-label) being oppositely arranged and the funnel 1 of bottom opening (non-label), is arranged on the funnel 1
Bottom with the seal 102 of the bottom opening of closing funnel 1, the working electrode 2 being arranged on the upper surface of the seal 102,
Be embedded on the funnel 1 to electrode 3, the lid 40 of the top opening of the closing funnel 1, can relatively described working electrode 2
Mobile magnet 5 and reference electrode 6.The funnel 1 includes inwall 11 and the funnel chamber 12 for setting and being formed is enclosed by inwall 11.In this reality
Apply in example, the lid 40 is column structure, the electrochemical luminescence bioanalysis flow cell 100 based on magnetic bead can be divided into
Three bulks, bottom cylinder 10, middle part cylinder 20 and the lid 40 set successively including superposition from bottom to up, the bottom cylinder 10,
Middle part cylinder 20 and lid 40 are (not shown) fixed by fastener respectively, and the funnel 1 is formed in the middle part cylinder 20,
The bottom of the middle part cylinder 20 sets fluted 202, and the seal 102 is to protrude out to be formed convex upwards from bottom cylinder 10
Platform, the seal 102 coordinates to realize the bottom opening of closing funnel 1 with groove 202, in order to realize optimum efficiency, in this implementation
In example, the working electrode 2 on the upper surface of seal 102 shelters from the bottom opening of funnel 1, to improve the sensitive of detection
Degree, certainly, in other embodiments, the working electrode 2 can not exclusively shelter from the bottom opening of funnel 1, as long as it is located at
The underface of the bottom opening of funnel 1, still, the detection sensitivity of such a structure do not have the detection sensitivity in the present embodiment
It is high.The magnet 5 is fixed on bottom cylinder 10, and the fluting 103 for housing magnet 5 is offered on the bottom cylinder 10, described
Fluting 103, which runs through in the bottom surface 104 of bottom cylinder 10, the fluting 103, is additionally provided with moving member 105, and the magnet 5 is arranged on
On moving member 105, when specifically used, after sample is entered in funnel chamber 12, by promote moving member 105 by magnet 5 to
The direction of working electrode 2 is moved, so that the antigen antibody complex with magnetic bead is adsorbed on working electrode 2., should in the present embodiment
Fluting 103 is screwed hole, and moving member 105 is bolt.In the present embodiment, by the driving of moving member 105, the magnet 5 is with respect to work
Make electrode 2 to move up and down, in other embodiments, the moving member 105 can also be other structures, block such as be taken out, in addition, passing through
Change the mounting means of moving member 105, to change the moving direction of magnet 5, such as magnet 5 is moved with respect to working electrode or so
It is dynamic.The reference electrode 6 is fixed on the cylinder 20 of middle part, and it on middle part cylinder 20 by opening up consolidating for fixed reference electrode 6
Determine groove 61 to realize fixation.
The axial rings to electrode 3 along funnel 1 around and be embedded in funnel 1, in the present embodiment, to electrode 3 by conduction
Material, which encloses, to be set to form funnel-shaped structure.It is described that electrode connection is respectively equipped with to electrode 3 and working electrode 2, wherein, on electrode 3
Electrode connection be named as to electrode connection 71, the electrode connection on working electrode 2 is named as working electrode wiring 72, by this
It is in the present embodiment, described to the cloth of electrode connection 71 to electrode connection 71 and working electrode wiring 72 to be connected with external power source
Put in middle part cylinder 20, and stretched out from the side wall 201 of middle part cylinder 20, the working electrode wiring 72 is arranged in bottom cylinder
In 10, working electrode wiring interface 73 is provided with the side wall 101 of the bottom cylinder 10, working electrode wiring 72 enters should
Working electrode wiring interface 73.It is described to offering perforate 31 on electrode 3, one end of the reference electrode 6 is arranged on described open
On hole 31.
Liquid access way is offered on the lid 40, the funnel chamber 12 is connected by liquid access way and outside
Logical, the liquid access way is mainly used in passing in and out the coreaction liquid and cleaning solution of ECL bioanalysis, can be also used for certainly into
Sample, in the present embodiment, is connected with pipeline 43 on the liquid access way, and the liquid access way is two, including enters
Liquid passage 44 and liquid outlet channel 45, really, the liquid access way may also be only one, or multiple.The lid 40
With the plunger portion 46 stretched in the funnel chamber 12, there is detention space when preventing and rinsing so that rinse more thorough.Institute
Stating feed pathway 44 has the feed liquor opening 441 being opened in the plunger portion 46, and the liquid outlet channel 45, which has, is opened in institute
State and go out liquid opening 451 in plunger portion 46, the feed liquor opening 441 goes out liquid opening 451, in the present embodiment, institute less than described in
Feed liquor opening 441 is stated to recline to formation between the inwall 11 of the funnel 1, and the feed liquor opening 441 and the inwall 11 of funnel 1
The gap flowed through for sample, coreaction liquid, cleaning solution, the plunger portion 46 is in circular platform type, it is described go out liquid opening 451 be along described
The spiral slot that the side wall 201 of plunger portion 46 extends.The optical channel 41 of the lid 40, institute are provided through on the lid 40
State optical channel 41 to connect with funnel chamber 12, optical mirror slip 8 is provided with the optical channel 41, can be by the optical mirror slip 8
When flushing, prevent coreaction liquid or cleaning solution from being flowed out out of optical channel 41, so as to rinse more thorough.In the present embodiment,
The bottom surface of the lid 40 is arranged with recess 42, and the recess 42 is arranged in the plunger portion 46, and the optical mirror slip 8 is arranged on
In the recess 42, to reduce the overall volume of the electrochemical luminescence bioanalysis flow cell 100 based on magnetic bead, in addition, in reality
It is specially optics convex lens to apply optical mirror slip 8 used in example, really, can also use planar wave eyeglass.
The electrochemical luminescence bioanalysis flow cell 100 based on magnetic bead (is hereinafter referred to as circulated below by experimental result
Pond 100) illustrate
Experiment one：With 125fM Ru (bpy)3 2+/ TPA solution enters as checking matter to the sample injection time of the flow cell 100
Row is investigated.Rinsed first with flushing liquor TPA, then blowing air drains flushing liquor, last sample introduction, in different time points as much as possible
Detect the ECL of checking matter.With reference to as shown in figure 5, checking matter ECL intensity is first quick with the increase of sample injection time in preceding 40s
Increase, because checking matter progresses into detection architecture, replaces the washing lotion TPA not drained, and its concentration in flow cell 100 is continuous
Increase, luminous intensity is continuously increased；Because checking matter replaces most TPA completely after 40s, the content of flow cell 100 is stabilization
The 125fM Ru (bpy) of component3 2+/ TPA solution, its ECL intensity tends towards stability and reaches peak.Therefore, it is guarantee checking matter
Displacement TPA completely circulates pond 100, so that it is determined that using 45s as optimal sample injection time.
Experiment two：Using TPA as flushing liquor, to above-mentioned 125fM Ru (bpy)3 2+/ TPA detection liquid is rinsed, to investigate
The washing time of the flow cell 100.Fig. 6 is the ECL intensity levels of remaining checking matter in flow cell 100 under different washing times.With
TPA continuous flushing, ECL intensity levels are constantly reduced, i.e., remaining checking matter concentration is constantly reduced in flow cell 100, after 40s
ECL responses are almost reduced to zero.Therefore, to ensure that flow cell 100 is rinsed completely, so that it is determined that using 45s as optimal washing time.
Refer to Fig. 7 and combine Fig. 1 to Fig. 4, the electrochemical luminescence based on magnetic bead described in the embodiment of the present invention two is biological
Analyze the basic phase of structure of flow cell and the electrochemical luminescence bioanalysis flow cell 100 based on magnetic bead described in embodiment one
Together, difference is：In embodiment one, optical channel 41 is to be through to bottom surface from the top surface of lid 40；And in embodiment two, institute
The same bottom surface that lid 40 ' is through to from the side of the lid 40 ' of optical channel 41 ' is stated, but is provided with the optical channel 41 '
Optical fiber 9 ', one end of the optical fiber 9 ' is connected with optics convex lens 8.In addition, in embodiment one, when placing sample every time, needing
Lid 40 is removed, or injected by feed pathway 44；And in embodiment two, inserting and moving for the ease of sample
Remove, sample holes 47 ' are offered on the lid 40 ', the funnel chamber 12 ' passes through the sample holes 47 ' and ft connection, institute
State and rubber stopper 48 ' is provided with sample holes 47 ', the sample holes 47 ' are formed from bifurcated in the feed pathway 44 ', and its is specific
Structure is：The sample holes 47 ' have shared major trunk roads with feed pathway 44 ', and sample holes 47 ' and feed pathway 44 ' are also divided
The sample introduction point arterial highway of bifurcated formation and feed liquor arterial highway Ju You not be divided from major trunk roads.
Except above-mentioned two is implemented to make an exception, the optical channel can be through to the bottom wall of lid from the side wall of the lid, should
Have in optical channel on corner, the corner and be provided with reflective mirror, certainly, optical fiber can also be equally installed in optical channel, this
When, then reflective mirror need not be set.
In summary, the above-mentioned electrochemical luminescence bioanalysis flow cell 100 based on magnetic bead will can be carried by magnet 5
The antigen antibody complex of magnetic bead is adsorbed on working electrode 2, is then injected coreaction liquid by liquid access way and is washed
Wash, the detection to antigen antibody complex is realized finally by working electrode 2, to electrode 3 and optical channel 41,41 ', with existing skill
Art is compared, should the entirety miniaturization of electrochemical luminescence bioanalysis flow cell 100 based on magnetic bead, electrochemical luminescence bioanalysis stream
The effective volume that funnel chamber 12,12 ' is shown in the logical self structure of pond 100 is smaller, in addition, compared with prior art, being used for
The ECL reaction reagents of bioanalysis are relatively fewer, that is, the ECL reaction reagents consumed are relatively fewer.
Because the feed liquor opening 441 of feed pathway 44 less than liquid outlet channel 45 goes out liquid opening 451, to keep unidirectional stream,
Rinse well, it is to avoid the result of influence detection, what is more, because feed liquor opening 441 reclines to the inwall 11, go out liquid opening
451 be along the plunger portion 46 side wall 201 extend spiral slot, and plunger portion 46 design, so as to be more conducive to avoid
Dead angle is rinsed, the result for avoiding influence from detecting is more conducive to.
In addition, by setting sample holes 47 ' on lid 40 ' so that detection sample Rapid replacement, and also contribute to
Improve the service life of the electrochemical luminescence bioanalysis flow cell based on magnetic bead.Simultaneously as the bottom opening of sample holes 47 '
For feed liquor opening 441 ', and the feed liquor opening 441 ' reclines to the inwall 11 ', and uses hopper design, so as to insert sample
During product, sample can be distributed on working electrode 2 ' along inwall 11 ', be favorably improved detection speed, it helps improve detection spirit
Sensitivity, realizes quick analysis.
Described above is only the preferred embodiment of the present invention, is not intended to limit the invention, it is noted that for this skill
For the those of ordinary skill in art field, without departing from the technical principles of the invention, can also make it is some improvement and
Modification, these improvement and modification also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of electrochemical luminescence bioanalysis flow cell based on magnetic bead, it is characterised in that：Including with the top being oppositely arranged
Opening and bottom opening funnel, be arranged on the funnel bottom with the seal of the bottom opening of closing funnel, be arranged on it is described close
Working electrode on the upper surface of sealing, be embedded on the funnel to electrode, the lid of the top opening of the closing funnel
And the magnet that can be moved relative to the working electrode, the axial rings to electrode along funnel around and be embedded in funnel, the leakage
Bucket includes inwall and optical channel that the lid is provided through on the funnel chamber for setting and being formed, the lid is enclosed by inwall, described
Optical channel is connected with funnel chamber, is provided with the optical channel on optical mirror slip, the lid and is offered liquid access way, institute
State funnel chamber and pass through liquid access way and ft connection.
2. the electrochemical luminescence bioanalysis flow cell according to claim 1 based on magnetic bead, it is characterised in that：The liquid
Body access way includes feed pathway and liquid outlet channel, and the lid has the plunger portion stretched in the funnel chamber, described
Feed pathway has the feed liquor opening being opened in the plunger portion, and the liquid outlet channel, which has, to be opened in the plunger portion
Go out liquid opening, the feed liquor opening goes out liquid opening less than described in.
3. the electrochemical luminescence bioanalysis flow cell according to claim 2 based on magnetic bead, it is characterised in that：It is described enter
Liquid opening plaster is leaned on to the inwall, and the plunger portion is in circular platform type, it is described go out liquid opening be to extend along the side wall of the plunger portion
Spiral slot.
4. the electrochemical luminescence bioanalysis flow cell according to claim 1 based on magnetic bead, it is characterised in that：The base
Also include reference electrode in the electrochemical luminescence bioanalysis flow cell of magnetic bead, it is described to offering perforate, the ginseng on electrode
One end than electrode is arranged in the perforate.
5. the electrochemical luminescence bioanalysis flow cell according to claim 4 based on magnetic bead, it is characterised in that：The base
Include middle part cylinder in the electrochemical luminescence bioanalysis flow cell of magnetic bead, the funnel formation is in the middle part cylinder, institute
State the fixing groove that the fixed reference electrode is offered on the cylinder of middle part.
6. the electrochemical luminescence bioanalysis flow cell according to claim 5 based on magnetic bead, it is characterised in that：The base
Include being located at the bottom cylinder below the middle part cylinder, the middle part post in the electrochemical luminescence bioanalysis flow cell of magnetic bead
The bottom of body sets fluted, and the seal is to protrude out the boss to be formed upwards from the bottom cylinder, the seal with
The groove coordinates, and the fluting for housing the magnet is offered on the bottom cylinder, and the fluting runs through the bottom cylinder
Bottom surface, be additionally provided with moving member in the fluting, the magnet is arranged on the moving member.
7. the electrochemical luminescence bioanalysis flow cell based on magnetic bead according to any one in Claims 2 or 3, its
It is characterised by：Sample holes are offered on the lid, the funnel chamber passes through the sample holes and ft connection, the sample holes
On be provided with sealing-plug.
8. the electrochemical luminescence bioanalysis flow cell according to claim 7 based on magnetic bead, it is characterised in that：It is described enter
Sample hole is formed from bifurcated in the feed pathway.
9. the electrochemical luminescence bioanalysis flow cell according to claim 1 based on magnetic bead, it is characterised in that：The electricity
Chemiluminescence flow cell also includes the optical fiber being arranged in the optical channel.
10. the electrochemical luminescence bioanalysis flow cell according to claim 1 based on magnetic bead, it is characterised in that：It is described
Optical channel, which is through to from the side wall of the lid in the bottom wall of lid, the optical channel, has corner, is provided with the corner
Reflective mirror.
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| CN201510345054.6A CN104950026B (en) | 2015-06-19 | 2015-06-19 | Electrochemical luminescence bioanalysis flow cell based on magnetic bead |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510345054.6A CN104950026B (en) | 2015-06-19 | 2015-06-19 | Electrochemical luminescence bioanalysis flow cell based on magnetic bead |
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| CN104950026B true CN104950026B (en) | 2017-07-28 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105806828B (en) * | 2015-10-29 | 2019-05-14 | 北京联众泰克科技有限公司 | A kind of Electrogenerated chemiluminescent immunoassay system and its flow cell component |
| CN106556594A (en) * | 2016-12-07 | 2017-04-05 | 中国人民解放军第三军医大学第附属医院 | Magnetic control electrogenerated chemiluminescence flow detection pond |
| CN114487387A (en) * | 2022-01-24 | 2022-05-13 | 北京铂茵生物科技有限公司 | Electrochemical luminescence reaction cup, analysis method and optical detection device |
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| CN1387036A (en) * | 2002-07-09 | 2002-12-25 | 中国科学院长春应用化学研究所 | Capillary electrophoresis and flow injection universal electrochemical light flux detecting pool |
| CN102066934A (en) * | 2008-06-12 | 2011-05-18 | 株式会社日立高新技术 | Analyzer using magnetic particles |
| CN203720142U (en) * | 2014-03-07 | 2014-07-16 | 中国水产科学研究院 | Miniaturized electrochemical three-electrode system detection pond |
| CN104538168A (en) * | 2014-12-23 | 2015-04-22 | 苏州大学 | Magnetic bead preparing method and application |
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| JP6239243B2 (en) * | 2013-02-08 | 2017-11-29 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Automatic analyzer |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1387036A (en) * | 2002-07-09 | 2002-12-25 | 中国科学院长春应用化学研究所 | Capillary electrophoresis and flow injection universal electrochemical light flux detecting pool |
| CN102066934A (en) * | 2008-06-12 | 2011-05-18 | 株式会社日立高新技术 | Analyzer using magnetic particles |
| CN203720142U (en) * | 2014-03-07 | 2014-07-16 | 中国水产科学研究院 | Miniaturized electrochemical three-electrode system detection pond |
| CN104538168A (en) * | 2014-12-23 | 2015-04-22 | 苏州大学 | Magnetic bead preparing method and application |
Non-Patent Citations (1)
| Title |
|---|
| Polyelectrolyte-based electrochemiluminescence enhancement for Ru(bpy)32+ loaded by SiO2 nanoparticle carrier and its high sensitive immunoassay;Zhi-Li Ge等;《Analytica Chimica Acta》;20150112;第862卷;第24–32页 * |
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