CN104945463A - Extracting method for tea saponin - Google Patents
Extracting method for tea saponin Download PDFInfo
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- CN104945463A CN104945463A CN201510454084.0A CN201510454084A CN104945463A CN 104945463 A CN104945463 A CN 104945463A CN 201510454084 A CN201510454084 A CN 201510454084A CN 104945463 A CN104945463 A CN 104945463A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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Abstract
The invention provides an extracting method for tea saponin. The extracting method comprises the following steps: a) mixing tea seed cakes with an extracting solution to be subjected to crude extraction treatment, so as to obtain a tea saponin extracting solution; b) mixing the tea saponin extracting solution with a sterols complexing agent to be subjected to complexing treatment, so as to obtain a tea saponin complex; c) mixing the tea saponin complex and a decomplexing solvent to be subjected to decomplexing separation treatment, so as to obtain the tea saponin. Compared with the prior art, the extracting method provided by the invention has the advantages that tea saponin and the sterols complexing agent are subjected to complexing to obtain the solid through the imprinting effects, and other impurities are not subjected to complexing, so that the separation on the chemical reaction level is realized; further, the high-purity tea saponin is obtained through decomplexing. A detecting result shows that the purity of the tea saponin obtained through the extracting method reaches as high as 98% or above, and the extraction ratio can reach 30%.
Description
Technical field
The present invention relates to technological field of biochemistry, more particularly, relate to a kind of extracting method of tea saponin.
Background technology
Tea saponin has another name called TS; by the class candy glucoside compound extracted in tea tree seed; it is a kind of natural surface active agent of good performance; it can be widely used in the fields such as light industry, chemical industry, agricultural chemicals, feed, cultivation, weaving, oil recovery, mining, building materials and expressway construction, manufactures emulsifying agent, cleaning agent, inert ingredient, fodder additives, crab shrimp aquaculture protective material, textile auxiliary agent, oilfield foaming agent, mining flotation agent and gas concrete suds-stabilizing agent and concrete admixture---frostproofer etc.
China's tea resource is very abundant, and wherein camellia oleifera lam area is about fifty-five million mu, and containing a large amount of tea saponin, tea oil and protein etc. in the leached tea oil slag obtained after the oil expression of tea seed, has very wide application prospect and considerable economic benefit.But due to the restriction of tea saponin extractive technique, leached tea oil slag does not also obtain rational development and utilization, apart from exporting to outside the country such as Japan and South East Asia on a small quantity, most of leached tea oil slag is burnt as waste material.
At present, extracting method mainly organic solvent method and the water seaoning of tea saponin, and the novel method promoted on this basis, as water extraction-precipitator method, water extraction-ol extraction method, resin adsorption method, supersonic method, membrane separation process etc.But aforesaid method all utilizes the dissolubility difference of the impurity such as tea saponin and protein in different solvents, realizes the effect of separation and Extraction in physical reaction aspect, and the extraction yield of tea saponin is low; The more important thing is, extract that the tea saponin purity that obtains is low, shade deviation and unstable properties, product only can use as thick tea saponin, lacks competitiveness, and limits the application of product to a great extent.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of extracting method of tea saponin, the tea saponin purity that extracting method provided by the invention obtains is good and extraction yield is high.
The invention provides a kind of extracting method of tea saponin, comprise the following steps:
A) leached tea oil slag is mixed with extracting solution, slightly propose process, obtain tea saponin extract;
B) described tea saponin extract is mixed with sterols complexing agent, carry out complexing process, obtain tea saponin complex compound;
C) by described tea saponin complex compound and decomplexing solvent, carry out decomplexing separating treatment, obtain tea saponin.
Preferably, step a) described in extracting solution be one or more in methyl alcohol, ethanol, water, n-propyl alcohol, Virahol and butanols.
Preferably, step a) described in the amount ratio of leached tea oil slag and extracting solution be 1g:(1mL ~ 12mL).
Preferably, step a) described in slightly propose process process specifically comprise the following steps:
Carry out successively after leached tea oil slag is mixed with extracting solution heating, filtering, obtain tea saponin extract;
The temperature of described heating is 50 DEG C ~ 110 DEG C, and the time is 1h ~ 3h.
Preferably, step b) described in sterols complexing agent be cholesterol, one or more in Stigmasterol and Sitosterol.
Preferably, step b) described in the process of complexing process specifically comprise the following steps:
Carry out successively after described tea saponin extract is mixed with sterols complexing agent heating, concentrating and filter, obtain tea saponin complex compound;
The temperature of described heating is 50 DEG C ~ 110 DEG C, and the time is 4h ~ 6h.
Preferably, step c) described in separate sequester be toluene, hexanaphthene, one or more in isopropyl ether and sherwood oil.
Preferably, step c) described in tea saponin complex compound with to separate the amount ratio of sequester be 1g:(0.7mL ~ 8mL).
Preferably, step c) described in the process of decomplexing separating treatment specifically comprise the following steps:
Heat carrying out successively after described tea saponin complex compound and decomplexing solvent, filter, obtain tea saponin;
The temperature of described heating is 50 DEG C ~ 120 DEG C, and the time is 1h ~ 3h.
Preferably, step a) described in leached tea oil slag is mixed with extracting solution before, also comprise:
Leached tea oil slag is crushed to 80 order ~ 120 orders.
The invention provides a kind of extracting method of tea saponin, comprise the following steps: a) leached tea oil slag is mixed with extracting solution, slightly propose process, obtain tea saponin extract; B) described tea saponin extract is mixed with sterols complexing agent, carry out complexing process, obtain tea saponin complex compound; C) by described tea saponin complex compound and decomplexing solvent, carry out decomplexing separating treatment, obtain tea saponin.Compared with prior art, extracting method provided by the invention, by trace effect, make tea saponin and sterols complexing agent complexes obtain solid, and there is not complexing in other impurity, thus realize the separation of chemical reaction aspect, then obtain highly purified tea saponin by decomplexing.Detected result shows, the tea saponin high purity more than 98% that extracting method provided by the invention obtains, extraction yield can reach 30%.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described technical scheme of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The invention provides a kind of extracting method of tea saponin, comprise the following steps:
A) leached tea oil slag is mixed with extracting solution, slightly propose process, obtain tea saponin extract;
B) described tea saponin extract is mixed with sterols complexing agent, carry out complexing process, obtain tea saponin complex compound;
C) by described tea saponin complex compound and decomplexing solvent, carry out decomplexing separating treatment, obtain tea saponin.
In the present invention, leached tea oil slag is mixed with extracting solution, slightly propose process, obtain tea saponin extract.In the present invention, described leached tea oil slag is obtained after extracting oil, containing compositions such as tea saponin, tea oil and protein in described leached tea oil slag by Theaceae, Camellia Plants seed; The source of the present invention to described leached tea oil slag is not particularly limited, and is preferably camellia seed meal.In the present invention, before leached tea oil slag is mixed with extracting solution, preferably also comprise:
Leached tea oil slag is crushed to 80 order ~ 120 orders.In the present invention, the object pulverized by leached tea oil slag is conducive to extracting solution fully to contact with leached tea oil slag, is convenient to slightly propose process.The equipment of the present invention to described pulverizing is not particularly limited, and adopts pulverizer well known to those skilled in the art.In the present invention, the order number that described leached tea oil slag is pulverized is preferably 80 order ~ 120 orders, is more preferably 90 order ~ 110 orders, most preferably is 100 orders.
In the present invention, described extracting solution be preferably in methyl alcohol, ethanol, water, n-propyl alcohol, Virahol and butanols one or more, be more preferably methyl alcohol, ethanol or Virahol, most preferably be Virahol.The source of the present invention to described extracting solution is not particularly limited, and adopts the commercial goods of above-mentioned methyl alcohol well known to those skilled in the art, ethanol, n-propyl alcohol, Virahol and butanols.In the present invention, described extracting solution is comparatively large to the solubleness of the tea saponin in leached tea oil slag, less to other the solubleness as impurity such as protein, thus realizes the thick extraction to tea saponin in leached tea oil slag.
In the present invention, the amount ratio of described leached tea oil slag and extracting solution is preferably 1g:(1mL ~ 12mL), be more preferably 1g:(3mL ~ 6mL), most preferably be 1g:5mL.
In the present invention, the described process slightly proposing process preferably specifically comprises the following steps:
Carry out successively after leached tea oil slag is mixed with extracting solution heating, filtering, obtain tea saponin extract.In the present invention, the process of described heating is preferably carried out under the condition stirred, and object promotes the dissolving of tea saponin in extracting solution in leached tea oil slag.In the present invention, the temperature of described heating is preferably 50 DEG C ~ 110 DEG C, is more preferably 60 DEG C ~ 100 DEG C, most preferably is 100 DEG C; The time of described heating is preferably 1h ~ 3h, is more preferably 2h.
After completing described heating, the mixture after heating filters by the present invention, obtains tea saponin extract.In the present invention, described filtration is preferably heat filtering; Described heat filtering refers to and to filter on the temperature basis of heating, ensures that being dissolved in tea saponin in extracting solution can not separate out because temperature reduces.
In the present invention, the process of described filtration obtains filtrate and filter residue respectively, and wherein, described filtrate is tea saponin extract, and described filter residue preferably proceeds slightly to propose process 1 ~ 2 time, and object is fully extracted by a small amount of residual tea saponin in filter residue.
After obtaining described tea saponin extract, described tea saponin extract mixes with sterols complexing agent by the present invention, carries out complexing process, obtains tea saponin complex compound.In the present invention, described sterols complexing agent is preferably cholesterol, one or more in Stigmasterol and Sitosterol, is more preferably Stigmasterol.The source of the present invention to described sterols complexing agent is not particularly limited, and adopts above-mentioned cholesterol well known to those skilled in the art, the commercial goods of Stigmasterol and Sitosterol.In the present invention, described sterols complexing agent can with the tea saponin in tea saponin extract by trace effect, and complexing obtains solid---tea saponin complex compound, and there is not complexing in other impurity, thus realize the further extraction and isolation to tea saponin extract.
In the present invention, the process of described complexing process preferably specifically comprises the following steps:
Carry out successively after described tea saponin extract is mixed with sterols complexing agent heating, concentrating and filter, obtain tea saponin complex compound.In the present invention, the process of described heating is preferably carried out under the condition stirred, and object promotes tea saponin and sterols complexing agent generation complexing in tea saponin extract, separates out uniform tea saponin complex compound, and sterols complexing agent crystal is dissolved completely.In the present invention, the temperature of described heating is preferably 50 DEG C ~ 110 DEG C, is more preferably 60 DEG C ~ 100 DEG C, most preferably is 100 DEG C; The time of described heating is preferably 4h ~ 6h, is more preferably 5h.
After completing described heating, the mixture after heating concentrates by the present invention.In the present invention, described concentrated object is convenient to carrying out smoothly of subsequent filter process; Described concentrated process preferably carries out vacuum concentration under cryogenic, and object obtains more tea saponin complex compounds.In the present invention, described concentrated temperature is preferably-5 DEG C ~ 5 DEG C, is more preferably 0 DEG C; The described concentrated time is preferably 1h ~ 3h, is more preferably 2h.
Complete described concentrated after, the mixture after concentrated filters by the present invention, obtains tea saponin complex compound.In the present invention, described filtration is preferably suction filtration; The solid that described suction filtration obtains is tea saponin complex compound, and the filtrate that described suction filtration process obtains can be used as the extracting solution slightly putting forward treating processes and reclaims use.
After obtaining described tea saponin complex compound, the present invention, by described tea saponin complex compound and decomplexing solvent, carries out decomplexing separating treatment, obtains tea saponin.In the present invention, described solution sequester is preferably toluene, hexanaphthene, one or more in isopropyl ether and sherwood oil, is more preferably toluene, isopropyl ether or sherwood oil, most preferably is sherwood oil.The source of the present invention to described solution sequester is not particularly limited, and adopts above-mentioned toluene well known to those skilled in the art, hexanaphthene, the commercial goods of isopropyl ether and sherwood oil.In the present invention, the tea saponin in tea saponin complex compound and sterols complexing agent can be carried out decomplexing by described solution sequester, and are dissolved by described sterols complexing agent, and tea saponin is insoluble in solution sequester, thus realize separating effect.
In the present invention, described tea saponin complex compound is preferably 1g:(0.7mL ~ 8mL with the amount ratio separating sequester), be more preferably 1g:(1.5mL ~ 3.5mL).
In the present invention, the process of described decomplexing separating treatment preferably specifically comprises the following steps:
Heat carrying out successively after described tea saponin complex compound and decomplexing solvent, filter, obtain tea saponin.In the present invention, the process of described heating is preferably carried out under the condition stirred, and object promotes that tea saponin complex compound fully contacts with solution sequester, realizes decomplexing.In the present invention, the temperature of described heating is preferably 50 DEG C ~ 120 DEG C, is more preferably 60 DEG C ~ 110 DEG C, most preferably is 60 DEG C; The time of described heating is preferably 1h ~ 3h, is more preferably 2h.
After completing described heating, the mixture after heating filters by the present invention, obtains tea saponin.In the present invention, described filtration is preferably heat filtering; Described heat filtering refers to and to filter on the temperature basis of heating, and ensureing to be dissolved in the sterols complexing agent separated in sequester can not separate out because temperature reduces.
After obtaining described tea saponin, the present invention preferably also comprises:
Described tea saponin is carried out decomplexing separating treatment 1 ~ 2 time.In the present invention, repeatedly carrying out decomplexing separating treatment fully can be separated a small amount of sterols complexing agent remained in tea saponin, improves the purity of tea saponin further.In the present invention, the filtrate that the process that described decomplexing is separated obtains preferably through further concentrated obtain decomplexing solvent recuperation after continue to use; Described concentrated after the solid that obtains reclaim after continue to use as sterols complexing agent.
The invention provides a kind of extracting method of tea saponin, comprise the following steps: a) leached tea oil slag is mixed with extracting solution, slightly propose process, obtain tea saponin extract; B) described tea saponin extract is mixed with sterols complexing agent, carry out complexing process, obtain tea saponin complex compound; C) by described tea saponin complex compound and decomplexing solvent, carry out decomplexing separating treatment, obtain tea saponin.Compared with prior art, extracting method provided by the invention, by trace effect, make tea saponin and sterols complexing agent complexes obtain solid, and there is not complexing in other impurity, thus realize the separation of chemical reaction aspect, then obtain highly purified tea saponin by decomplexing.Detected result shows, the tea saponin high purity more than 98% that extracting method provided by the invention obtains, and product color is good, stable performance, and the extraction yield of tea saponin can reach 30% simultaneously.
In addition, the extracting method technological operation of tea saponin provided by the invention is simple and production energy consumption is low, and the product that each step obtains can recycle, and cost is lower, is applicable to suitability for industrialized production.
In order to further illustrate the present invention, be described in detail below by following examples.Following examples of the present invention camellia seed meal used is provided by Hunan Jinhao Camellia Oil Co., Ltd.; Sherwood oil used is provided by Nanchang Jinsha Chemical Co., Ltd., technical grade, and boiling point is 60 DEG C ~ 80 DEG C.
Embodiment 1
(1) 100g is milled to 100 object camellia seed meals to mix with 500mL Virahol, at 100 DEG C, stirs 2h, then through heat filtering, obtain tea saponin extract.
(2) above-mentioned tea saponin extract is mixed with 27g Stigmasterol, at 100 DEG C, stir 5h, then carry out vacuum concentration 2h at 0 DEG C, dry through suction filtration, obtain 61g tea saponin complex compound.
(3) mixed with 200mL sherwood oil by above-mentioned tea saponin complex compound, at 60 DEG C, stir 2h, then through heat filtering, obtain 29g tea saponin, the extraction yield calculating tea saponin is 29%.
Adopt the purity of Shimadzu LC10 type high performance liquid chromatograph to tea saponin to detect, testing conditions is:
Moving phase is methyl alcohol: water=40:60;
Wavelength is 280nm;
Flow velocity is 1.0mL/min;
Sample size is 20 μ L;
Chromatographic column is Shimadzu WondaSil C18,4.6mm × 150mm × 5 μm.
Detected result shows, the purity 98.0% of the tea saponin that the extracting method of the tea saponin that the embodiment of the present invention 1 provides obtains.
Embodiment 2
(1) 100g is milled to 100 object camellia seed meals to mix with 500mL Virahol, at 100 DEG C, stirs 2h, then through heat filtering, obtain tea saponin extract A and filter residue A respectively;
Then, described filter residue A is mixed with 400mL Virahol, at 100 DEG C, stirs 2h, then through heat filtering, obtain tea saponin extract B and filter residue B respectively;
Finally, described filter residue B is mixed with 300mL Virahol, at 100 DEG C, stirs 2h, then through heat filtering, after obtaining tea saponin extract C, merge with tea saponin extract A and tea saponin extract B and obtain tea saponin extract.
(2) above-mentioned tea saponin extract is mixed with 32g Stigmasterol, at 100 DEG C, stir 5h, then carry out vacuum concentration 2h at 0 DEG C, dry through suction filtration, obtain 62g tea saponin complex compound.
(3) above-mentioned tea saponin complex compound is mixed with 200mL sherwood oil, at 60 DEG C, stir 2h, then through heat filtering, obtain tea saponin A;
Then, described tea saponin A is mixed with 150mL sherwood oil, at 60 DEG C, stirs 2h, then through heat filtering, obtain tea saponin B;
Finally, mixed by described tea saponin B, at 60 DEG C, stir 2h with 80mL sherwood oil, then through heat filtering, obtain 28g tea saponin, the extraction yield calculating tea saponin is 28%.
Detect the purity of tea saponin, detected result shows, the purity 98.3% of the tea saponin that the extracting method of the tea saponin that the embodiment of the present invention 2 provides obtains.
Embodiment 3
(1) 100g is milled to 110 object camellia seed meals and 300mL methanol mixed, at 60 DEG C, stirs 2h, then through heat filtering, obtain tea saponin extract A and filter residue A respectively;
Then, by described filter residue A and 200mL methanol mixed, at 60 DEG C, stir 2h, then through heat filtering, obtain tea saponin extract B and filter residue B respectively;
Finally, by described filter residue B and 100mL methanol mixed, at 60 DEG C, stir 2h, then through heat filtering, after obtaining tea saponin extract C, merge with tea saponin extract A and tea saponin extract B and obtain tea saponin extract.
(2) above-mentioned tea saponin extract is mixed with 28g cholesterol, at 60 DEG C, stir 5h, then carry out vacuum concentration 2h at 0 DEG C, dry through suction filtration, obtain 56g tea saponin complex compound.
(3) above-mentioned tea saponin complex compound is mixed with 100mL toluene, at 110 DEG C, stir 2h, then through heat filtering, obtain tea saponin A;
Then, described tea saponin A is mixed with 60mL toluene, at 110 DEG C, stirs 2h, then through heat filtering, obtain tea saponin B;
Finally, mixed by described tea saponin B, at 110 DEG C, stir 2h with 40mL toluene, then through heat filtering, obtain 30g tea saponin, the extraction yield calculating tea saponin is 30%.
Detect the purity of tea saponin, detected result shows, the purity 98.2% of the tea saponin that the extracting method of the tea saponin that the embodiment of the present invention 3 provides obtains.
Embodiment 4
(1) 100g is milled to 90 object camellia seed meals to mix with 400mL ethanol, at 78 DEG C, stirs 2h, then through heat filtering, obtain tea saponin extract A and filter residue A respectively;
Then, described filter residue A is mixed with 300mL ethanol, at 78 DEG C, stirs 2h, then through heat filtering, obtain tea saponin extract B and filter residue B respectively;
Finally, described filter residue B is mixed with 200mL ethanol, at 78 DEG C, stirs 2h, then through heat filtering, after obtaining tea saponin extract C, merge with tea saponin extract A and tea saponin extract B and obtain tea saponin extract.
(2) above-mentioned tea saponin extract is mixed with 30g Sitosterol, at 78 DEG C, stir 5h, then carry out vacuum concentration 2h at 0 DEG C, dry through suction filtration, obtain 60g tea saponin complex compound.
(3) above-mentioned tea saponin complex compound is mixed with 180mL isopropyl ether, at 60 DEG C, stir 2h, then through heat filtering, obtain tea saponin A;
Then, described tea saponin A is mixed with 100mL isopropyl ether, at 60 DEG C, stirs 2h, then through heat filtering, obtain tea saponin B;
Finally, mixed by described tea saponin B, at 60 DEG C, stir 2h with 80mL sherwood oil, then through heat filtering, obtain 29g tea saponin, the extraction yield calculating tea saponin is 29%.
Detect the purity of tea saponin, detected result shows, the purity 98.2% of the tea saponin that the extracting method of the tea saponin that the embodiment of the present invention 4 provides obtains.
The above-mentioned explanation of the disclosed embodiments, enables professional and technical personnel in the field realize or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (10)
1. an extracting method for tea saponin, is characterized in that, comprises the following steps:
A) leached tea oil slag is mixed with extracting solution, slightly propose process, obtain tea saponin extract;
B) described tea saponin extract is mixed with sterols complexing agent, carry out complexing process, obtain tea saponin complex compound;
C) by described tea saponin complex compound and decomplexing solvent, carry out decomplexing separating treatment, obtain tea saponin.
2. extracting method according to claim 1, is characterized in that, step a) described in extracting solution be one or more in methyl alcohol, ethanol, water, n-propyl alcohol, Virahol and butanols.
3. extracting method according to claim 1, is characterized in that, step a) described in the amount ratio of leached tea oil slag and extracting solution be 1g:(1mL ~ 12mL).
4. extracting method according to claim 1, is characterized in that, step a) described in slightly propose process process specifically comprise the following steps:
Carry out successively after leached tea oil slag is mixed with extracting solution heating, filtering, obtain tea saponin extract;
The temperature of described heating is 50 DEG C ~ 110 DEG C, and the time is 1h ~ 3h.
5. extracting method according to claim 1, is characterized in that, step b) described in sterols complexing agent be cholesterol, one or more in Stigmasterol and Sitosterol.
6. extracting method according to claim 1, is characterized in that, step b) described in the process of complexing process specifically comprise the following steps:
Carry out successively after described tea saponin extract is mixed with sterols complexing agent heating, concentrating and filter, obtain tea saponin complex compound;
The temperature of described heating is 50 DEG C ~ 110 DEG C, and the time is 4h ~ 6h.
7. extracting method according to claim 1, is characterized in that, step c) described in separate sequester be toluene, hexanaphthene, one or more in isopropyl ether and sherwood oil.
8. extracting method according to claim 1, is characterized in that, step c) described in tea saponin complex compound with to separate the amount ratio of sequester be 1g:(0.7mL ~ 8mL).
9. extracting method according to claim 1, is characterized in that, step c) described in the process of decomplexing separating treatment specifically comprise the following steps:
Heat carrying out successively after described tea saponin complex compound and decomplexing solvent, filter, obtain tea saponin;
The temperature of described heating is 50 DEG C ~ 120 DEG C, and the time is 1h ~ 3h.
10. the extracting method according to any one of claim 1 ~ 9, is characterized in that, step a) described in leached tea oil slag is mixed with extracting solution before, also comprise:
Leached tea oil slag is crushed to 80 order ~ 120 orders.
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| CN106188214A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of simple and easy method purifying tea saponin |
| CN106188213A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of Simple Purification method of tea saponin |
| CN106234774A (en) * | 2016-08-01 | 2016-12-21 | 南宁市泽威尔饲料有限责任公司 | The preparation method of sucrose magnesium complex |
| CN108997467A (en) * | 2018-07-11 | 2018-12-14 | 永州职业技术学院 | A method of Tea Saponin is extracted using biotechnology |
| CN109897727A (en) * | 2019-03-08 | 2019-06-18 | 石城县群鑫生态发展有限公司 | A kind of tea-seed oil leaching extraction process |
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| CN106188213A (en) * | 2016-07-19 | 2016-12-07 | 陕西科技大学 | A kind of Simple Purification method of tea saponin |
| CN106188214B (en) * | 2016-07-19 | 2018-04-06 | 陕西科技大学 | A kind of simple and easy method for purifying Tea Saponin |
| CN106234774A (en) * | 2016-08-01 | 2016-12-21 | 南宁市泽威尔饲料有限责任公司 | The preparation method of sucrose magnesium complex |
| CN108997467A (en) * | 2018-07-11 | 2018-12-14 | 永州职业技术学院 | A method of Tea Saponin is extracted using biotechnology |
| CN109897727A (en) * | 2019-03-08 | 2019-06-18 | 石城县群鑫生态发展有限公司 | A kind of tea-seed oil leaching extraction process |
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