CN104940191A - Fat soluble epigallocatechin gallate-palmitate disinfectant and preparation method - Google Patents
Fat soluble epigallocatechin gallate-palmitate disinfectant and preparation method Download PDFInfo
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Abstract
The invention discloses a fat soluble epigallocatechin gallate-palmitate disinfectant and a preparation method. The disinfectant is formed by mixing of an organic solution and an inorganic solution. The organic solution contains glycerin, benzyl alcohol, gluconolactone, vitamin E, and fat soluble epigallocatechin gallate-palmitate. The inorganic solution contains deionized water, citric acid, sodium benzoate, Carbomer, hydroxyethyl cellulose, and an aloe extract. The preparation steps include: 1. preparing an organic solution, i.e. adding fat soluble epigallocatechin gallate-palmitate and auxiliary materials, and mixing the materials uniformly to obtain the organic solution; 2. preparing the inorganic solution, i.e. dissolving citric acid, sodium benzoate, Carbomer, hydroxyethyl cellulose and the aloe extract in deionized water, adjusting the pH value to obtain a jelly solution; and 3. adding the organic solution into the inorganic solution to obtain the disinfectant. The disinfectant provided by the invention has the advantages of reasonable formula, convenient use, long term efficacy, high efficiency, no toxicity, and oxidation resistance, does not contain contaminants or toxic substances, and is suitable for infants, children and adults.
Description
Technical field
The present invention relates to medical art, more specifically relate to a kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant (Epigallocatechin gallate-Palmitate, EGCG-p), also relate to a kind of preparation method of fat-soluble nutgall catechin gallic acid cetylate disinfectant simultaneously, can be used on family as surface disinfectant, hospital, school, public place, poultry cultivation field, animal husbandry, and other may suffer the place of viral infection.Not only kill already present antibacterial and virus in time, in long-time, keep antiviral effect simultaneously.Background technology
Epigallocatechin gallate (EGCG) (Epigallocatechin gallate, EGCG) is the most effective active component in tea polyphenols, belongs to catechin.EGCG has antibacterial, antiviral, antioxidation, arteriosclerosis, antithrombus formation, antiangiogenic, antiinflammatory and antitumor action, due to its hypotoxicity and the native compound be present in green tea, it is made to become a kind of development object with very big captivation.Epigallocatechin gallate (EGCG) (EGCG) is a kind of composition extracted from Chinese green tea, it is the main activity of green tea and water-soluble components, it is the component that in catechin, content is the highest, account for the 9%-13% of green tea gross weight, because have special stereochemical structure, EGCG has very strong antioxidant activity, antioxidant activity is at least ascorbic more than 100 times, it is 25 times of vitamin E, can Cell protection and DNA undermined, this infringement is believed and cancer, heart disease is relevant with other major diseases, these effects of EGCG ascribe their removing (antioxidation) ability to oxygen-derived free radicals to.EGCG appears on the inventory of FDA as safe consumption product.
But EGCG is water soluble molecules and unstable, very easily oxidized, cannot make the medicine that stability is strong.Doctor Xu De carried out modification with regard to proposing to tea polyphenols/EGCG as far back as 2006, and then strengthened its stability, fat-soluble, particularly to the activity of viral kill.This new concept obtains confirmation in international new drug development field.Modification EGCG is eager to excel tens times than not having the EGCG of modification to the killing effect of virus.
The known road EGCG of people has the effect suppressing virus before this, but direct drinks green tea, EGCG can decompose at once in vivo, cannot play a role.EGCG is unstable in water, and easily oxidation causes loss.For this reason, ours is be proved to be the fat-soluble EGCG-p(through modification with antiviral activity to combine fatty acid cetylate Palmitate).Not only water miscible EGCG modification is become fat-soluble, improve the stability of compound simultaneously.Due to the raising of stability, reduce and decompose and be oxidized thus the loss caused, EGCG-p substantially increases the activity of kill virus.
China disinfectant market does not have the long-acting product preventing viral infection at present.At bird flu, simple spore rash, hand-foot-mouth disease disease, hepatitis B etc. take place frequently, cause in the environment of death, our this of research and development is that the mechanism of action of the disinfectant solution prophylaxis of viral infections of one of main component is all different from the disinfectant that current any market is sold with EGCG-p, is characterized in long-acting, efficiently, nontoxic.Long-actingly refer to each for after dermal application or surface sprinkling, in 2 hours, can prevent multiple viral infection from infecting (action time of ethanol is 14 seconds).Efficiently refer to its valid density very low, deactivation can be carried out to multiple virus.Nontoxic is that its toxicity is even lower than green tea because of the esterification modification of new compound from natural molecule.All above-mentioned contents are all by a large amount of experiment in early stage, comprise that human trial gets, and have many sections of doctors Xu De and other research teams to be published in research report on International Periodicals as basis.
The feature adopting the disinfectant solution of novel form is long-acting, nontoxic, pollution-free.For the prevention of hand-foot-mouth disease, in the occasion that child assembles, such as kindergarten, the popularizations such as primary school.For the prevention of influenza, school will be generalized to, hospital, the places such as unit.What another emphasis prevented is the virus infected by property, as simple spore rash, and human papilloma virus, HIV (human immunodeficiency virus) etc.
Novel long-acting nontoxic pollution-free disinfectant solution is mainly used in skin care and surface sterilization, prevents hand-foot-mouth disease virus, and influenza (comprising bird flu) virus, hepatitis virus, spore exanthema virus, HIV (human immunodeficiency virus) etc. are to the infection of human body.Add the demand (reaching annual 21700000000 dollars for 2010) of animal husbandry disinfectant solution produce market.The long-effective protection agent that this product will be the multiple viral infection of a kind of strick precaution completely newly.Its market prospect is boundless.
Summary of the invention
The object of the invention is to there are provided a kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, formula is reasonable, easy to use.Be characterized in long-acting, efficiently, nontoxic.Long-actingly refer to each for after dermal application or surface sprinkling, in 2 hours, can prevent multiple viral infection from infecting (action time of ethanol is 14 seconds).Efficiently refer to its valid density very low, deactivation can be carried out to multiple virus.Nontoxic is that its toxicity is even lower than green tea because of the esterification modification of new compound from natural molecule.And to skin, there is protective effect, do not pollute the environment.
Another object of the present invention is the preparation method that there are provided a kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, method is feasible, easy and simple to handle, not containing pollutant and noxious substance, can be made into disinfectant solution, containing disinfectant solution napkin, spray agent, wipe hands liquid, dermal application agent, lubricant etc.Baby, child, can use safely per capita with becoming.With other products unlike long-acting, efficiently, nontoxic, and play protection skin, antioxidative effect.
In order to realize above-mentioned object, the present invention adopts following technical measures:
Its technical conceive is: the present invention is a kind of disinfectant solution, and a kind of disinfectant solution that is Main Function composition with fat-soluble nutgall catechin gallic acid cetylate, is mixed by organic solution and inorganic solution.For kill bacteria and virus.Organic solution can contain glycerol, benzyl alcohol, gluconic acid lactone (anticorrosion), vitamin E (antioxidation), fat-soluble nutgall catechin gallic acid cetylate.Inorganic solution can contain deionized water, citric acid (antisepsis and sterilization), sodium benzoate (antisepsis and sterilization), carbomer, hydroxyethyl-cellulose, Aloe extract etc.
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up of the raw material of following weight portion:
Parts by weight of raw materials
Organic solution accounts for the 10-20 of cumulative volume.
Inorganic solution accounts for the 80-90 of cumulative volume.
Described organic solution can contain glycerol, benzyl alcohol, gluconic acid lactone (anticorrosion), vitamin E (antioxidation), any mixing of fat-soluble nutgall catechin gallic acid cetylate wherein a kind of or two to five kinds.
Described inorganic solution can contain deionized water, citric acid (antisepsis and sterilization), sodium benzoate (antisepsis and sterilization), carbomer, hydroxyethyl-cellulose, any mixing of Aloe extract wherein a kind of or two to six kinds.
Deionized water accounts for the 75-85 of cumulative volume, and glycerol accounts for the 8-12 of cumulative volume, and fat-soluble nutgall catechin gallic acid cetylate accounts for the 0.01-5 of cumulative volume.Citric acid accounts for the 0.1-5 of cumulative volume, and sodium benzoate accounts for the 0.1-5 of cumulative volume.Benzyl alcohol accounts for the 0.1-5 of cumulative volume, and carbomer accounts for the 0.1-0.5 of cumulative volume.Aloe extract accounts for the 0.1-0.5 of cumulative volume, and hydroxyethyl-cellulose accounts for the 0.1-5 of cumulative volume, and vitamin E accounts for the 0.1-0.5 of cumulative volume.
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up of the raw material of following weight portion:
Parts by weight of raw materials
Deionized water (or containing ethanol) 75-85
Glycerol 8-12
Fat-soluble nutgall catechin gallic acid cetylate 0.01-5
Citric acid 0.1-5
Sodium benzoate 0.1-5
Benzyl alcohol 0.1-5
Carbomer 0.1-5
Hydroxyethyl-cellulose 0.1-5
Aloe extract 0.1-0.5
Vitamin E 0.1-0.5
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up (preferable range) of the raw material of following weight portion:
Parts by weight of raw materials
Deionized water 76-84
Glycerol 9-11
Fat-soluble nutgall catechin gallic acid cetylate 0.1-0.5
Citric acid 0.2-4
Sodium benzoate 0.2-4
Benzyl alcohol 0.2-4
Carbomer 0.2-2
Hydroxyethyl-cellulose 0.1-3
Aloe extract 0.2-0.4
Vitamin E 0.2-0.4
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up (best scope) of the raw material of following weight portion:
Parts by weight of raw materials
Deionized water 78-82
Glycerol 10-10.5
Fat-soluble nutgall catechin gallic acid cetylate 0.2-1
Citric acid 0.3-3
Sodium benzoate 0.3-3
Benzyl alcohol 0.3-3
Carbomer 0.25-0.3
Hydroxyethyl-cellulose 0.5-2
Aloe extract 0.25-0.3
Vitamin E 0.25-0.3
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up (optimum) of the raw material of following weight portion:
Parts by weight of raw materials
Deionized water 82
Glycerol 10
Fat-soluble nutgall catechin gallic acid cetylate 0.2
Citric acid 0.3
Sodium benzoate 1
Benzyl alcohol 1
Carbomer 0.5
Hydroxyethyl-cellulose 0.5
Aloe extract 0.2
Vitamin E 0.1.
A preparation method for fat-soluble nutgall catechin gallic acid cetylate disinfectant, the steps include:
1, organic solution is prepared: get 1 ~ 5ml benzyl alcohol, after adding fat-soluble nutgall catechin gallic acid cetylate 0.1 ~ 5g and adjuvant Homogeneous phase mixing, be mixed to get organic solution with 10 ~ 15ml glycerol again.
2, prepare inorganic solution: citric acid, sodium benzoate, carbomer, hydroxyethyl-cellulose, Aloe extract, to be dissolved in 75ml ~ 85ml ionized water.Regulate acid-base value to pH-7.0 to pH-8.0.Solution agglutination.
3, organic solution slowly joined in inorganic solution, limit edged is stirred to uniform solution, obtains the one fat-soluble nutgall catechin gallic acid cetylate disinfectant of 100ml.
Described adjuvant is citric acid, any composition of a kind of in benzyl alcohol and sodium benzoate four kinds or two to four kinds;
Described solvent is any mixing of benzyl alcohol and glycerol wherein a kind of or two kinds.
This for fat-soluble nutgall catechin gallic acid cetylate long-acting, nontoxic disinfectant component dissolves in nontoxic, free of contamination Glycerol solvents, is made the nontoxic non-stimulated disinfectant with long-effective protection ability by the present invention.
The present invention compared with prior art, has the following advantages and effect:
A, formula are reasonable, easy to use.Be characterized in long-acting, efficiently, nontoxic.
B, long-actingly refer to each for after dermal application or surface sprinkling, in 2 hours, can prevent multiple viral infection from infecting (action time of ethanol is 14 seconds).Deactivation is carried out to multiple virus.Nontoxic is that its toxicity is even lower than green tea because of the esterification modification of new compound from natural molecule.And to skin, there is protective effect, do not pollute the environment.
C, method are feasible, easy and simple to handle, not containing pollutant and noxious substance, can be made into disinfectant solution, and containing disinfectant solution napkin, spray agent, wipes hands liquid, dermal application agent, lubricant etc.
D, can to use safely per capita baby, child and becoming.With other products unlike long-acting, efficiently, nontoxic, and play protection skin, antioxidative effect.
This formula and the maximum difference of other products are by the composition of powerful kill virus-fat-soluble nutgall catechin gallic acid cetylate-be attached to Skin Cell surface or other material surface for a long time, make virally inactivated, cannot infection cell.The sterile products made has the effect of efficient kill bacteria and virus, and fat-soluble nutgall catechin gallic acid cetylate forms protecting film at skin surface, and the virus effectiveness that goes out, more than two hours, does not stimulate skin.
Detailed description of the invention
Embodiment 1:
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up of the raw material of following weight portion:
Parts by weight of raw materials
Deionized water 84.4
Glycerol 9
Fat-soluble nutgall catechin gallic acid cetylate 0.2
Citric acid 2
Sodium benzoate 1.5
Benzyl alcohol 1.5
Carbomer 0.5
Hydroxyethyl-cellulose 0.5
Aloe extract 0.3
Vitamin E 0.1.
A preparation method for fat-soluble nutgall catechin gallic acid cetylate disinfectant, the steps include:
1, prepare organic solution: get 1 or 2 or 3 or 4 or 5ml benzyl alcohol, after adding fat-soluble nutgall catechin gallic acid cetylate 0.1 or 0.5 or 1 or 2 or 3 or 4 or 5g and adjuvant Homogeneous phase mixing, be mixed to get organic solution with 10 or 12 or 13 or 15ml glycerol again.
2, inorganic solution is prepared: carbomer is dissolved in 75ml or 79 or 83 or 85ml ionized water.Regulate acid-base value to pH6.5 or 6.9 or 7.4. add the citric acid be dissolved in deionized water, sodium benzoate, Aloe extract,
3, organic solution slowly joined in inorganic solution, limit edged is stirred to uniform solution, obtains the one fat-soluble nutgall catechin gallic acid cetylate disinfectant of 100ml.
A kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up of the raw material of following weight portion:
| Embodiment | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| Deionized water | 84.59 | 84.55 | 84.5 | 84.1 | 83.6 | 83.1 | 82.1 |
| Glycerol | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
| Fat-soluble nutgall catechin gallic acid cetylate | 0.01 | 0.05 | 0.1 | 0.5 | 1.0 | 1.5 | 2 |
| Citric acid | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| Sodium benzoate | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Benzyl alcohol | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Carbomer | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Hydroxyethyl-cellulose | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Aloe extract | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 | 0.3 |
| Vitamin E | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 | 0.1 |
| Amount to | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
its preparation process is identical with embodiment 1.
This product contains two kinds of formula of fat-soluble tea polyphenol-and fat-soluble nutgall catechin gallic acid cetylate.One is spirituosity (ethanol), another kind of soft.For surface disinfectant.
The disinfectant that this product contains fat-soluble tea polyphenol-and fat-soluble nutgall catechin gallic acid cetylate infects for prophylaxis of microbial, particularly the infection of virus on human and animal.
The disinfectant that this product contains fat-soluble tea polyphenol-and fat-soluble nutgall catechin gallic acid cetylate is used for preventing microorganism to the infection of human and animal as long-acting surface disinfectant.
This product contains the viral infection of disinfectant for preventative propagation of fat-soluble tea polyphenol-and fat-soluble nutgall catechin gallic acid cetylate.
The disinfectant that this product contains fat-soluble tea polyphenol-and fat-soluble nutgall catechin gallic acid cetylate for by the skin of viral infection, mucosa.Reach later stage disinfective action.
This product contains the formula of the disinfectant of fat-soluble tea polyphenol-and fat-soluble nutgall catechin gallic acid cetylate for different product kind.Such as wipe hands liquid, disinfected paper napkin, surface sterilization spray agent, skin surface liniment, skin lubrication agent, medical disinfectant etc.
Embodiment 9: disinfectant solution is stored in the stability test of 25 DEG C:
(humidity 75%) storage 6 months in disinfectant 48 degree of couveuses, room temperature storage 2 years, detects once the content of fat-soluble nutgall catechin gallic acid cetylate every half a year, the results are shown in Table 1
It is as shown in the table that table 1 embodiment of the present invention disinfectant solution is stored in the stability test of 25 DEG C:
Experimental result shows this disinfectant character and stablizes, effective antimicrobial activity stable components.
Embodiment 10: in " sterile products inspection technology specification " that disinfectant solution is issued by former Ministry of Public Health the detection of the bactericidal effect of escherichia coli and staphylococcus aureus, method of regulation is carried out, specific as follows:
One, experiment purpose: detect the bactericidal effect of disinfectant solution to escherichia coli and staphylococcus aureus.
Two, sample source: test sample EGCG cetylate disinfectant solution is product prepared by the embodiment of the present invention 1,2,3,4,5,6,7,8.
Three, experimental procedure
1, test equipment
1) strain: staphylococcus aureus ATCC 6538, escherichia coli 8099,
2) phosphate buffer: see appendix A.
3) sterile distilled water.
4) disinfectant dilution hard water: see appendix A.
5) organic interfering substance matter: suspension test 3%BSA solution.
6) TSA culture medium: tryptose soya agar culture medium (TSA) is shown in appendix A.
7) containing the pancreas peptone soybean broth culture medium (nertralizer TSB) of nertralizer, nertralizer is through qualified.
8) measuring pipette (1.0ml, 5.0ml, 10.0ml), capillary pipette.
9) constant water bath box.
10) motorized agitator.
11) stopwatch.
12) digital adjustable pipette (10 μ l, 20 μ l, 100 μ l, 200 μ l, 1000 μ l) and supporting disposable plastic suction nozzle.
13) centrifuge.
14) scopometer.
2, bacterial suspension preparation
1) get freeze-drying lactobacillus pipe, open under sterile working, add adequate nutrition meat soup with capillary pipette, soft pressure-vaccum for several times, makes strain melt dispersion.Get containing 5.0 ml ~ 10.0ml nutrient broth medium test tubes, instill a little bacteria suspension, put 37 DEG C and cultivate 18h ~ 24h.Get the bacteria suspension of 1st generation cultivation with inoculating loop, streak inoculation, on nutrient agar flat board, cultivates 18h ~ 24h in 37 DEG C.Colonies typical in the above-mentioned 2nd culture thing of picking, is inoculated in nutrient agar slopes, cultivates 18h ~ 24h, be the 3rd culture thing in 37 DEG C.
2) get strain the 3rd generation ~ nutrient agar inclined-plane fresh cultured thing (18h ~ 24h) in 14 generations, draw 3.0 ml ~ 5.0ml diluents with 5.0ml suction pipe and add in slant tube, pressure-vaccum repeatedly, washes lower lawn.Subsequently, with 5.0ml suction pipe, washing liquid is moved in another sterile test tube, with motorized agitator mixing (vibration) 20s, or shake on palm and strike 80 times, to make bacterial suspension even.
3) bacteria suspension tentatively made, first with the bigness scale of bacterial concentration nephelometry its containing bacteria concentration, then with diluted to the concentration of required use.
4) bacterial propagule suspension should be kept in 4 DEG C of refrigerators for subsequent use.Should must not use and spend the night in sky.
3, test grouping
Following group should be divided in test:
(1) test group.Optional test bacterium and a decontaminant concentration effect 10min test.
(2) positive controls.According to the regulation of various test, replace disinfectant solution with diluent, test by above-mentioned same step.Acquired results represents the original concentration of bacterium liquid, using it as calculating the initial concentration killing logarithm.
4, Suspension quantitative bactericidal test operation sequence
1) use Sterile Hard Water preparation disinfectant solution test sample, the concentration of preparation is 1.25 times of concentration to be measured, and it is for subsequent use to put 20 DEG C ± 1 DEG C water-bath.
2) according to 2 preparation experiment bacteria suspensions, concentration is 1 × 10
8cfu/ml ~ 5 × 10
8cfu/ml.
3) get the aseptic Boiling tube of sterilizing test, first add 0.5ml test bacteria suspension, then add 0.5ml organic interfering substance matter, mixing, put in 20 DEG C ± 1 DEG C water-bath after 5min, draw above-mentioned concentration disinfectant solution 4.0 ml inject wherein with aseptic straw, mixing is also clocked immediately rapidly.
4) bacterium to be tested and disinfectant interact to each scheduled time, and absorption 0.5ml test organisms and disinfectant mixed liquor are added on 4.5ml in the nertralizer of sterilizing respectively, mixing.
5) each pipe test organisms and disinfectant mixed liquor are after adding nertralizer effect 10min, draw 1.0ml sample liquid respectively, and cultivate method of counting mensuration by viable bacteria and deposit viable count, often pipe sample liquid inoculates 2 plates.When clump count as grow on plates is more, after series 10 times dilution can be carried out, then carry out viable bacteria cultivation counting.
6) replace disinfectant solution with diluent simultaneously, carry out parallel test, as positive control.
7) all test samples are all cultivated in 37 DEG C of incubators, cultivate 48h observe final result to bacterial propagule.
(8) test repetition 3 times, calculate the viable bacteria concentration (cfu/ml) of each group, and be scaled logarithm value (N), be then calculated as follows kill oncomelania:
Logarithm value (the No)-test group viable bacteria concentration logarithm value (Nx) of kill oncomelania (KL)=matched group mean viable concentration
5, embodiment of the present invention disinfectant solution to the bactericidal effect of escherichia coli and staphylococcus aureus in table 2
Table 2: embodiment disinfectant solution is to the killing effect of escherichia coli and staphylococcus aureus
| Sample | Action time (min) | To escherichia coli kill oncomelania | To staphylococcus aureus kill oncomelania |
| Embodiment 1 | 10 | 6.1 | 6.5 |
| Embodiment 2 | 10 | 6.0 | 6.6 |
| Embodiment 3 | 10 | 6.2 | 6.5 |
| Embodiment 4 | 10 | 6.1 | 6.5 |
| Embodiment 5 | 10 | 6.1 | 6.6 |
| Embodiment 6 | 10 | 6.0 | 6.7 |
| Embodiment 7 | 10 | 6.2 | 6.5 |
| Embodiment 8 | 10 | 6.0 | 6.6 |
6, result evaluation:
This test is by the bactericidal effect of scraps of paper test method evaluation solution E GCG palmitic acid lipid disinfectant solution to escherichia coli and staphylococcus aureus, selected two kinds of bacterial strains are all modal pathogenic bacterium, are also Gram-negatives and Gram-positive is prevailing represents bacterium.As can be seen from the result of done laboratory observation, detected sample EGCG palmitic acid lipid disinfectant solution has clear and definite fungistatic effect to escherichia coli and staphylococcus aureus, and its bacteriostasis is seemingly stronger than Gram-negative (escherichia coli) to Gram-positive (staphylococcus aureus).Conclusion: EGCG palmitic acid lipid disinfectant solution has clear and definite bacteriostasis to escherichia coli and staphylococcus aureus.
The method of disinfectant sample to " sterile products inspection technology specification " middle regulation that the detection of poliovirus inactivating efficacy is issued by former Ministry of Public Health is carried out, specific as follows:
Experimental principle: the amount measuring poliovirus in disinfectant effect experimental group and matched group sample by cell infection method.Using cytopathy as judge index, determine the infection titer of each papova, calculate disinfectant to the inactivation ratio of poliovirus.Because in this experiment, the sample of disinfectant does not have corresponding nertralizer, so use in the physical method of dilution here and disinfectant effect, select cell maintenance medium as retarder thinner.
One, preliminary experiment:
1, cell maintenance medium+Vero cell → cultivation
Add cell maintenance medium, put in 37 DEG C of CO2 gas incubator cultivate 72 hours, every day observation of cell growth and morphology situation.
2, disinfectant+cell → cultivation
Experimental group cell is added respectively different dilution disinfectant (diluting with cell maintenance medium), act on after 2-3 hour, suck liquid, separately add cell maintenance medium, put in 37 DEG C of CO2 gas incubator cultivate 72 hours, every day observation of cell growth and morphology situation.
Note: by above-mentioned experimental verification Growth of Cells to the maximum tolerated concentration of disinfectant.
Five, sterilize experiment
1, disinfectant solution stock solution to be checked is diluted to Concentraton gradient to be measured (fix tentatively as original content, 10
-1, 10
-2) to put 20 DEG C ± 1 DEG C water-bath for subsequent use.
2, viral suspension is put 20 DEG C ± 1 DEG C water-bath 5min, take out 0.2mL viral suspension and mix timing with the disinfectant equal-volume of three kinds of concentration respectively; Act on to the stipulated time (first by 1,2,5,10min, look at that time is suitable, with this time for T, then be time gradient 1/2T, T, 3/2T), take out 0.1ml immediately, join mixing effect 10min in 0.9ml nertralizer, suitably dilution is inoculated in 96 porocyte plates, cultivate 72h, observe every day and record CPE.
3, positive control replaces disinfectant with PBS, operates by above-mentioned steps, adopts a time.Negative control is exactly cell hole flat board not adding virus.
Five data compilations
Each papova TCID50 logarithm value is calculated according to formula (1):
Kill oncomelania=Log (positive control TCID50)-Log (T papova TCID50) (1)
The results are shown in Table 4:
Table 4: embodiment disinfectant solution is to PV-I killing effect
| Action time (min) | Kill oncomelania | |
| Embodiment 1 | 10 | 4.6 |
| Embodiment 2 | 10 | 4.3 |
| Embodiment 3 | 10 | 4.5 |
| Embodiment 4 | 10 | 4.7 |
| Embodiment 5 | 10 | 4.8 |
| Embodiment 6 | 10 | 5.1 |
| Embodiment 7 | 10 | 5.3 |
| Embodiment 8 | 10 | 5.3 |
Result display EGCG palmitic acid lipid disinfectant solution has good killing action to poliovirus-I.
Above-described embodiment, the just one of the present invention's more preferably detailed description of the invention, the usual change that those skilled in the art carries out within the scope of technical solution of the present invention and replacing all should be included in protection scope of the present invention.
Claims (7)
1. a fat-soluble nutgall catechin gallic acid cetylate disinfectant, it is made up of the raw material of following weight portion:
Parts by weight of raw materials
Organic solution accounts for the 10-20 of cumulative volume
Inorganic solution accounts for the 80-90 of cumulative volume
Described organic solution contains any mixing of glycerol, benzyl alcohol, gluconic acid lactone, vitamin E, fat-soluble nutgall catechin gallic acid cetylate wherein a kind of or two to five kinds;
Described inorganic solution contains any mixing of deionized water, citric acid, sodium benzoate, carbomer, hydroxyethyl-cellulose, Aloe extract wherein a kind of or two to six kinds.
2. one according to claim 1 fat-soluble nutgall catechin gallic acid cetylate disinfectant, is characterized in that:
Parts by weight of raw materials
Deionized water 75-85
Glycerol 8-12
Fat-soluble nutgall catechin gallic acid cetylate 0.01-5
Citric acid 0.1-5
Sodium benzoate 0.1-5
Benzyl alcohol 0.1-5
Carbomer 0.1-5
Hydroxyethyl-cellulose 0.1-5
Aloe extract 0.1-0.5
Vitamin E 0.1-0.5.
3. one according to claim 1 fat-soluble nutgall catechin gallic acid cetylate disinfectant, is characterized in that:
Parts by weight of raw materials
Deionized water 76-84
Glycerol 9-11
Fat-soluble nutgall catechin gallic acid cetylate 0.1-0.5
Citric acid 0.2-4
Sodium benzoate 0.2-4
Benzyl alcohol 0.2-4
Carbomer 0.2-2
Hydroxyethyl-cellulose 0.1-3
Aloe extract 0.2-0.4
Vitamin E 0.2-0.4.
4. one according to claim 1 fat-soluble nutgall catechin gallic acid cetylate disinfectant, is characterized in that:
Parts by weight of raw materials
Deionized water 78-82
Glycerol 10-10.5
Fat-soluble nutgall catechin gallic acid cetylate 0.2-1
Citric acid 0.3-3
Sodium benzoate 0.3-3
Benzyl alcohol 0.3-3
Carbomer 0.25-0.3
Hydroxyethyl-cellulose 0.5-2
Aloe extract 0.25-0.3
Vitamin E 0.25-0.3.
5. one according to claim 1 fat-soluble nutgall catechin gallic acid cetylate disinfectant, is characterized in that:
Parts by weight of raw materials
Deionized water 82
Glycerol 10
Fat-soluble nutgall catechin gallic acid cetylate 0.2
Citric acid 0.3
Sodium benzoate 1
Benzyl alcohol 1
Carbomer 0.5
Hydroxyethyl-cellulose 0.5
Aloe extract 0.2
Vitamin E 0.1.
6. one according to claim 1 fat-soluble nutgall catechin gallic acid cetylate disinfectant, is characterized in that:
Parts by weight of raw materials
Deionized water 84.4
Glycerol 9
Fat-soluble nutgall catechin gallic acid cetylate 0.2
Citric acid 2
Sodium benzoate 1.5
Benzyl alcohol 1.5
Carbomer 0.5
Hydroxyethyl-cellulose 0.5
Aloe extract 0.3
Vitamin E 0.1.
7. the preparation method of a kind of fat-soluble nutgall catechin gallic acid cetylate disinfectant according to claim 1, the steps include:
A, preparation organic solution: get 1 ~ 5ml benzyl alcohol, after adding fat-soluble nutgall catechin gallic acid cetylate 0.1 ~ 5g and adjuvant Homogeneous phase mixing, be mixed to get organic solution with 10 ~ 15ml glycerol again;
B, preparation inorganic solution: citric acid, sodium benzoate, carbomer, hydroxyethyl-cellulose, Aloe extract, be dissolved in 75ml ~ 85ml ionized water, regulate acid-base value to pH-7.0 to pH-8.0, solution agglutination;
C, join in inorganic solution by organic solution, limit edged is stirred to uniform solution, obtains the one fat-soluble nutgall catechin gallic acid cetylate disinfectant of 100ml;
Described adjuvant is citric acid, any composition of a kind of in benzyl alcohol and sodium benzoate four kinds or two to four kinds;
Described solvent is any mixing of benzyl alcohol and glycerol wherein a kind of or two to two kinds.
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| CN105853676A (en) * | 2016-04-08 | 2016-08-17 | 武汉艾文斯生物科技有限公司 | Gallocatechin palmitate and alcohol compound disinfectant and preparation method thereof |
| CN110352992A (en) * | 2019-07-31 | 2019-10-22 | 长兴净安环保科技有限公司 | A kind of compound disinfectant based on QUAT12 degerming factor |
| IT201900019190A1 (en) | 2019-10-17 | 2021-04-17 | Univ Politecnica Delle Marche | DERIVATIVE OF EPIGALLOCATECHIN GALLATO |
| WO2021183878A1 (en) * | 2020-03-13 | 2021-09-16 | Augusta University Research Institute, Inc. | Compositions and methods for inhibiting endospores using green tea polyphenols |
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| CN102085159A (en) * | 2011-01-24 | 2011-06-08 | 长沙优威生物科技有限公司 | Nursing type disinfecting liquid soap |
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| CN105853676A (en) * | 2016-04-08 | 2016-08-17 | 武汉艾文斯生物科技有限公司 | Gallocatechin palmitate and alcohol compound disinfectant and preparation method thereof |
| CN110352992A (en) * | 2019-07-31 | 2019-10-22 | 长兴净安环保科技有限公司 | A kind of compound disinfectant based on QUAT12 degerming factor |
| IT201900019190A1 (en) | 2019-10-17 | 2021-04-17 | Univ Politecnica Delle Marche | DERIVATIVE OF EPIGALLOCATECHIN GALLATO |
| WO2021183878A1 (en) * | 2020-03-13 | 2021-09-16 | Augusta University Research Institute, Inc. | Compositions and methods for inhibiting endospores using green tea polyphenols |
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| CN104940191B (en) | 2018-10-12 |
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