CN104937390B - swab interface for microfluidic device - Google Patents
swab interface for microfluidic device Download PDFInfo
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- CN104937390B CN104937390B CN201380061295.7A CN201380061295A CN104937390B CN 104937390 B CN104937390 B CN 104937390B CN 201380061295 A CN201380061295 A CN 201380061295A CN 104937390 B CN104937390 B CN 104937390B
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- swab
- cavity
- port cavity
- port
- lid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/15—Absorbent pads, e.g. sanitary towels, swabs or tampons for external or internal application to the body; Supporting or fastening means therefor; Tampon applicators
- A61F13/38—Swabs having a stick-type handle, e.g. cotton tips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N2001/028—Sampling from a surface, swabbing, vaporising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N2001/1056—Disposable (single-use) samplers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
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Abstract
This disclosure relates to a kind of swab port device.Specifically, this disclosure relates to making swab port device that swab is docked on microfluidic device, by method that swab port is attached on the device, the method for making liquid be recycled on swab and enclosure system for sealing the device.
Description
This application claims enjoy in the excellent of the U.S. Provisional Patent Application submitted the 61/705,967th on the 26th of September in 2012
First weigh；It is fully incorporated in herein by reference.
Technical field
This disclosure relates to a kind of swab port device.Specifically, this disclosure relates to swab is made to be docked to microfluidic device
On swab port device, so that swab port is attached on the device method, so that liquid is recycled on swab method, with
And seal the enclosure system of the device.
Background technology
Many researchs and clinical assay carry out sample collection by swab or other disposable sample collecting devices.Example
Such as, heredity test, infectious disease testing (such as swab from body orifice) etc., all of sample collecting device.
Sample on swab is typically communicated to analytical equipment or component, for further testing.
Processing swab needs many steps and attachment, including scissors, pipeline, swirler and centrifuge at present.Additionally need
It is used for the additional device and method of the streaming removal sample from swab.
Invention content
This disclosure relates to a kind of swab port device.Specifically, this disclosure relates to swab is made to be docked to microfluidic device
On swab port device, so that swab port is attached on the device method, so that liquid is recycled on swab method, with
And seal the enclosure system of the device.
The embodiment provides a kind of swab port devices comprising at least one agent structure and lid, the master
Body structure includes one or more surfaces, which defines with upper part and low portion the first cavity and with top
Second cavity of part and low portion；Lid is configured to the first cavity of sealing and the second cavity.In certain embodiments, first is empty
Chamber is configured to receive sample collection swab in terms of size.In certain embodiments, the first cavity and the second cavity keep fluid
Connection.In certain embodiments, the first cavity includes (such as 0.50 to 5000 μ L, 50 to 1000 μ L, 50 to 500 about 300 μ L
μ L etc.) volume capacity.In certain embodiments, agent structure further includes multiple protrusions (such as different size or shape
Protrusion or foot), such as be configured to that the device is made to be aligned with the hole in analytical equipment.In certain embodiments, lid includes lid
Containment member and washer member.In certain embodiments, the first cavity includes neck.In certain embodiments, lid, which is integrated in, wipes
In subport device.
In certain embodiments, the first cavity includes one or more inner projections (such as tooth), is wiped to be conducive to work as
When son is in contact with protrusion, material is removed from swab for example, by shearing, scraping, shock or any other active force.
Further embodiment provides a kind of systems comprising：Swab port device described here；And with the device
The chemical examination component (such as microfluidic device) of connection.In certain embodiments, the protrusion of device is inserted in the hole in chemical examination component
In, and optionally, protrusion is heat sealed or is otherwise connected on chemical examination component.In certain embodiments, the system
Further include sample analysis component, is operatively coupled on chemical examination component.
In other other embodiments, the present invention provides a kind of methods comprising：A) make swab port device and change
It tests component to be in contact, swab port device includes i) at least one agent structure comprising one or more surfaces, surface limit
The first cavity with upper part and low portion and the second cavity with upper part and low portion；Ii) from master
The outstanding multiple protrusions in the bottom of body structure, wherein chemical examination component is configured to receive the hole of protrusion in terms of being included in size；b)
Make the heat that protrusion is melted on chemical examination component that device to be sealed on chemical examination component by application.
In other other embodiments, the present invention provides a kind of methods comprising：Make system described here and packet
The swab for including sample is in contact；Optionally fracture the end of swab so that the swab portion comprising sample is retained in the of device
In one cavity；And so that the liquid being included in the second cavity is passed through the first cavity and recycled that (such as so that sample is transferred to liquid
In body).In certain embodiments, this method further includes the step for making liquid be in contact with the component (such as microfluidic device)
Suddenly.In certain embodiments, this method further include identify sample in analyte (such as including but not limited to nucleic acid, propylhomoserin,
Lipid, metabolin or chemical analyte) the step of.
In certain embodiments, the purposes of above-mentioned apparatus or system is provided here.In certain embodiments, it provides here
The purposes of above-mentioned apparatus or system, for acquiring chemistry, biology or environmentally conscious materials from swab, such as diagnosing, screen,
Treatment or research purpose (such as the medical conditions of object or communicable diagnosis).
There has been described additional embodiments.
Description of the drawings
Specification provided herein may be better understood when reading in conjunction with the accompanying, attached drawing be as example and
It is unrestricted to be included.It should be understood that unless in addition indicated in context, otherwise similar label is in all the appended drawings
Identify similar component.It will also be appreciated that certain or all attached drawings may be schematic diagram for illustrative purposes, and
Not necessarily depict practical relative size or the position of shown element.
Fig. 1 shows a kind of illustrative swab port and the lid of embodiment of the disclosure.
Fig. 2 shows the exemplary elements for being sealed to swab port on analysis component.
Fig. 3 is shown for swab port being docked and being sealed to the exemplary elements on analysis component.
Fig. 4 shows the illustrative lid washer combinations of embodiment of the disclosure.
Fig. 5 shows the lid/washer docked with swab port.
Fig. 6 shows the swab in the swab port for being inserted into embodiment of the disclosure.
Fig. 7 shows the illustrative return port of embodiment of the disclosure.
Fig. 8 shows the recycling of the nucleic acid in recycling and swab by the solution of return port.
Fig. 9 shows the exemplary means of the embodiment of the present invention.
Figure 10 shows a kind of exemplary means including internal dentation, contributes to from the swab of insertion to remove
Material.
Definition
Before describing the present invention in detail, it is to be understood that the invention is not limited in specific device, system, external member or
Method can change.Unless in addition clearly stipulate that the otherwise list used in this specification and appended claims in context
Number form formula " one ", "one" and " this " include a plurality of objects.It will also be appreciated that term used herein above is used for the purpose of
The purpose of specific embodiment is described, it is not intended to limit it.In addition, unless in addition limit, it is otherwise used herein above
All technical and scientific terms are all identical with being commonly understood by with the those of ordinary skill in these fields of the present invention
Connotation.When describing and stating the claim of the present invention, the variant of following term and its grammer will be according to set forth below
It defines to use.
In the context of nucleic acid, term " amplification " or " amplification " refer to the multiple copies or polynucleotides of polynucleotides
The generation of a part, normally starts from a small amount of polynucleotides (such as single polynucleotide molecule), wherein amplified production or
Amplicon is typically detectable.The amplification of polynucleotides includes various chemical processes and enzymatic process.It is anti-in polymerase chain
It answers and is generated more by one or several copies of target DN or template DNA molecule during (PCR) or ligase chain reaction (LCR)
Weight DNA copies are the forms of amplification.Amplification is not limited to the duplication of stringent starting molecule.For example, by Finite Number in sample
It is a kind of amplification form that the RNA of amount generates multiple cDNA molecules using reverse transcription (RT)-PCR.In addition, during transcription
It is also a kind of amplification form to generate multiple RNA molecules by single DNA molecules.
Term " base content " refers to the quantity of each residue included in amplicon or other nucleic acid, does not account for this
A little linear arrays of the residue in expanding subchain.Amplicon residue includes adenosine (A), guanosine (G), cytidine, (C) (deoxidation) thymus gland
Pyrimidine (T), uracil (U), inosine (I), nitroindoline (such as 5- nitroindolines or 3- nitro-pyrroles), dP or dK (The U.S.Section
Institute of institute reports 95 (8):The paper of Hill F in 4258-63 et al. (1998) is " in conjunction with the synthesis of degeneration pyrimidine and purine bases
Oligodeoxynucleotide polymerase identification "), contain 5- nitro indazole rings the nucleoside analog (" nucleosides of Fan Aiershe top grade people
And nucleotide " 1995,14,1053-1056), purine analogue 1- (2- deoxidations-β-D-RIBOSE base)-imidazoles -4- formyls
Amine, 2,6-diaminopurine, 5- propynyluracils, 5- propynylcytosines, phenoxazine (including G- pincers), 5- propinyl deoxidations
Cytidine, deoxythymidine nucleotides, 5- propinyls cytidine, 5- propine and its quality status stamp modified forms, including 7- denitrogenations -2'- are de-
Oxygen adenosine -5- triphosphoric acids, the iodo- 2'- BrdUs -5'- triphosphoric acids of 5-, 5 bromo- 2'- BrdUs -5'- triphosphoric acids, the bromo- 2'- of 5-
Deoxycytidine -5'- triphosphoric acids, 5-iodo-2'-deoxycytidine -5'- triphosphoric acids, 5- hydroxyl -2'- BrdU -5'- triphosphoric acids, 4-
Thio thymidine-5'-triphosphate, 5- azepine -2'- BrdU -5'- triphosphoric acids, 5-fluoro-2'-deoxyuridine -5'- triphosphoric acids, O6
Methyl -2'- deoxyguanosine -5'- triphosphoric acids, N2Dimethyl -2'- deoxyguanosine -5'- triphosphoric acids, 8- oxo -2'- deoxidation birds
Glycosides -5'- triphosphoric acids or thio thymidine-5'-triphosphate.In certain embodiments, quality modified bases include15N or13C or15N
With13C.In certain embodiments, non-natural nucleosides used herein include 5- propynyluracils, 5- propynylcytosines and
Inosine.Here the base content for being used for unmodified DNA cloning is expressed as AwGxCyTz, wherein w, x, y and z is independent
Integer represents the quantity of the nucleotide residues in amplicon.Base content for amplicon includes modified nucleosides,
It is similarly represented, to indicate natural and modified nucleosides quantity described in amplicon.Point of the base content from amplicon
Protonatomic mass is calculated in measuring, as described below.Then any given amplicon is directed to by calculated base content
It is compared with the database of base content.Matching between the base content and single database entry of calculating discloses life
The identity characteristic of object preparation.
Term " connection " refers to direct or indirect transmission or transmission, and/or at least from some things to another things
The direct or indirect ability transmitted or transmit whatsit.When fluidity material or can be transferred to separately from jobbie
When one object, these objects " are in fluid communication " each other.For example, in certain embodiments of the present invention, swab port and reflux end
Mouth keeps being in fluid communication.
Term " external member " is used to refer to the combination of the processing for being conducive to sample, method, chemical examination, analysis or operation.External member can wrap
Containing description how using the external member, swab port, microfluidic device, lid, for thermosealed component, laboratory reagent and other
The guidance (such as describing the guidance of the method for the present invention) of component.External member component encapsulate possibly together in a reservoir (such as box,
Packet etc.), for loading onto ship, store or use, or two or more containers may be encapsulated in.
Term " material " refer to including substance or by material composition certain things.Term " fluidity material ", which refers to, to be tended to flow
The dynamic or material (such as liquid or gas) consistent with its container profile.
Term " minitype plate " fingerboard or other support constructions comprising a plurality of cavities or well, cavity or well are configured to receive
Material, such as fluidity material.Well is typically below about 1.5mL (such as about 1000 μ L, about 800 μ L, about 600
μ L, about 400 μ L or less) volume capacity, but certain minitype plates (such as deep-well plate etc.) have bigger volume capacity,
Such as about 4mL is per well.Minitype plate may include the well of various quantity, for example, 6,12,24,48,96,384
A, 1536,3456,9600 or more wells.In addition, the well of minitype plate typically comprises the matrix of rectangular matrix.It is miniature
Plate is typically compliant with the standard that the American National Standards Association (ANSI) of biomolecular screening association (SBS) is promulgated that represents, that is,
ANSI/SBS1-2004：Minitype plate-coverage area size, ANSI/SBS2-2004：Minitype plate-height dimension, ANSI/SBS3-
2004：Minitype plate-bottom outward flange size and ANSI/SBS4-2004：Minitype plate-well location is set, and is combined by reference
Herein.Minitype plate can be obtained from different manufacturers, including such as Gray receives u s company (Fla. Mary
Lake) and Nalge Nunc international corporations (USA New York Rochester) etc..Minitype plate is also commonly referred to as various different names, such as
" microtiter plate ", " micro- well plate ", " multi-well container " etc..
Term " molecular mass " refers to using measuring method of mass spectrum, for example, compound determined by ESI-MS quality.Here
Compound is preferably nucleic acid.In certain embodiments, nucleic acid is the nucleic acid (such as DNA nucleic acid of double-strand) of double-strand.In certain realities
It applies in example, nucleic acid is amplicon.When nucleic acid is double-strand, molecular mass is the determination for two chains.Implement at one
In example, chain can be separated before being introduced into mass spectrograph or chain can be separated by mass spectrograph (for example, electron spray ionisation will divide
Open hybridization chain).The molecular mass of each chain is measured by mass spectrograph.
Term " nucleic acid molecules " refers to any nucleic acid for including molecule, including but not limited to DNA or RNA.The term includes
Sequence comprising the base analogue of any of DNA and RNA, including but not limited to 4- acetylcytosines, 8- hydroxyls
N6- methyladenosines, '-aziridino cytimidine, false iso-cytosine, 5- (carboxy hydroxy methyl) uracil, 5 FU 5 fluorouracil, 5- bromines
Uracil, 5- carboxymethylamino methyl -2- thiouracils, 5- carboxymethyl aminomethyls uracil, dihydrouracil, inosine, N6- are different
Amylene adenine, 1- methyl adenines, 1- methyl puppet-uracil, 1- methyl guanines, 1-methylinosine, 2,2- dimethyl birds
Purine, 2- methyl adenines, 2- methyl guanines, 3- methylcysteins, 5-methylcytosine, N6- methyl adenines, 7- methyl
Guanine, 5- Methylaminomethyls uracil, 5- Methoxyamino methyl -2- thiouracils, β-D-MANNOSE, 5'- methoxyl groups
Carbonvlmethyl uracil, methyl uracil, 2- methyl mercapto-N- isopentennyladenines, uracil -5- oxy acetic acid methyls ester, urine
Pyrimidine -5- fluoroacetic acid, oxybutynin, pseudouracil, theophylline, 2- be thio, 5-methyl-2-thiouracil, 2- thiouracils, 4- sulphur
Uracil, methyl uracil, N- uracil -5- oxy acetic acid methyls ester, uracil -5- hydroxyacetic acids, pseudouracil, theophylline,
2- is thio and 2,6-diaminopurine.
Term " system " refers to one group of object and/or device, and which form the networks for executing required purpose.
Term " sample " used herein in its broadest sense to use.For from a certain meaning, meaning
Taste sample or culture including being obtained from any source, and biology and environmental samples.Biological sample can from animal (including
People) it obtains, and include fluid, solid, tissue and gas.Biological sample includes blood products, such as blood plasma, serum etc..
Environmental samples include environmentally conscious materials, such as surface mass, soil, water and industrial sample.However this example is not construed as limiting
The sample type for being applicable to the present invention is made.
Specific implementation mode
This disclosure relates to a kind of swab port device and the method and system using this swab port device.Specifically
It says, this disclosure relates to make swab port device that swab is docked on microfluidic device, swab port is made to be attached on the device
Method, so that liquid is recycled on swab method and seal the enclosure system of the device.
The embodiment provides the swab ports on a kind of microfluidic device so that user can be lost shape with swab band
This (such as detection of medical jurisprudence, clinic, biological warfare agent, environmental samples etc.), then portion removes it or with other in the device
Mode detaches it, and closes lid, to accommodate the swab and Liquid-treatment processes of downstream experience.As the embodiments described herein
A part for performed experiment during research has studied and (such as passes through method that swab port is adhered on device
Thermo-compression bonding).Thus, embodiment of the disclosure is further provided for being linked any modularizing member by thermal pressure welding method
Method onto microfluidic device or other component of a system.Appended experimental has investigated a kind of lid mechanism, is used as sealing swab
The method of port, and another part of identity unit is used as washer, to form sealing between microfluidic card and swab port.
Which increase the lid provided for keeping swab and any liquid of downstream experience is made to be retained in inside swab port, and this
Lid is connected on card the benefit of (such as preventing from losing unintentionally) always.
Exemplary embodiment is solved and how will be conventionally used to by removing labor-intensive step from workbench
The swab of medical jurisprudence, clinical application and explosive is docked to the problem on microfluidic device.Embodiment, which further provides, to be used for
Fracture the side of swab by method that modularizing member is attached on analytical equipment (such as microfluidic device), for portion in the device
Method, the enclosure system as washer and lid and the method for making fluent material flow back on swab during microfluidic procedures.
I. device
Various aspects of the disclosure will be described in further detail in following paragraphs.In brief, these include A) swab
Port；B) modularizing member (such as swab port) is thermally compressed on microfluidic device；C) begin to function as individual member but it
Whole, the enclosure system as washer and lid is constituted with port afterwards；And D) it is used for the return port of mixing/recycling outside plane.
A) swab port
Fig. 1 shows the schematic diagram of the swab port 1 in the case where lid is opened, closed, the sectional view of device when lid is closed
And the photo of the swab port on microfluidic card 5.Swab port 1 includes ontology 2, lid 3, swab insertion component 4 and recycling
Port 6 (will be made below being more fully described).
In order to use the device, user that the sample collecting device (such as swab) comprising sample is inserted into swab and is inserted into structure
In part 4.Then user fractures, cuts off or detaches otherwise is not inserted into the 4 closed swab portion of institute of component by swab, and closes
Lid 3 is closed, as shown in the second panel of Fig. 1.Then swab port 1 can be incorporated into such as micro fluidic plate 5, for analysis.
Swab port and microfluidic device can be constructed by any suitable material.In certain embodiments, device is logical
Cavity injection mold is crossed to be made of arylide or polystyrene, but other manufacturing methods and material be also can be specifically contemplated
's.
For example, in certain embodiments, utilizing mechanical processing, molding processing, extruding, punching press, engraving, injection molding, casting
It is moulded into shape, etching (such as chemical etching etc.) or other technologies and carrys out manufacturing device.Such as Molinari's et al. (Eds)
The manufacturing automation of metal cutting and high speed machine processing (academic presses Kluwer (2002)), Altintas:Metal cutting
Mechanical part, machine vibration and computer numerical control design the metal of (Cambridge University Press (2000)), Stephenson et al.
Cutting theory and put into practice (Marcel De Keer publishing houses (1997)), injection molding principle (W. J. T. alliances (2000)),
The injection molding volume 2 (Chapman and Hall publishing house (1991)) of the thermal plastic material of Whelan, the injection molding of Rosato
Handbook the 3rd edition (academic presses Kluwer (2000)), Fisher plastics squeeze (Holstead publishing house (1976)), with
And the extruding of the polymer of Chung：These and other conjunction is described in theory and practice (perseverance letter Gardner's publication (2000))
Suitable manufacturing technology, they are incorporated by reference herein.Illustrative material include, but are not limited to ABS,
Santoprene, HDPE, PEEK, TPE, LCP, PETG, TPV, Ultem, nylon, Udel, PBT, PVC, makrolon, Rdel,
Polymethyl methacrylate, polyethylene, dimethyl silicone polymer, polyether-ether-ketone, polytetrafluoroethylene (PTFE), polystyrene, polyvinyl chloride,
Polypropylene, polysulfones, polymethylpentene and makrolon etc..In certain embodiments, device is as hybrid station or related system
Disposable artifact or consumable component be fabricated.In certain embodiments, after the fabrication, the component of a system optionally into
Row is further processed, such as by (such as can be from Whitford companies (Pennsylvania with hydrophilic coating, hydrophobic coating
State west chester city) obtained resin 1010DF/870 carbon black coatings etc.) coating surface, to prevent for example in component surface
Interaction between reagent, sample etc..
Fig. 9 shows the device of the embodiment of the present invention.Panel 1) it is injection-molded swab port body and casting
The photo of silicon casing part, size is close to one penny.Transparent devices are made of acrylic resin, and opaque device is poly-
Styrene.2) top view of polystyrene swab port body shows main swab port holes (macropore on the left side) and reflux column
(the right).Also it can be seen that channel, swab port is connected in return port, to promote reflux course on top surface.3) phase
Same injection-molded design, but be made of acrylic materials rather than styrene.4) bottom view of molding part shows use
In shell (left side) small recess portion, and shell is placed on the inside of the recess portion on bottom surface.
Figure 10 shows the configuration of some embodiments of these devices, is used wherein the inner surface for receiving the cavity of swab has
In the protrusion for helping the removing material from the swab of insertion.Five pairs of dentations are shown in Fig. 10.However, protrusion may have
There are any desired shape or form, to obtain required result (round, rectangle etc.).Protrusion is preferably through configuration so that
If swab is placed into port by user, user can reverse swab, to help to remove cellular material from swab.It goes
Except can be completed for example, by grinding or scraping action.
B the method) being thermally compressed into modularizing member on microfluidic device
In certain embodiments, the present invention provides for component (such as swab port) to be thermally compressed into analysis component
Method on (such as microfluidic device).This has shown for example, in fig. 2.
Fig. 2 shows 1) foot's feature 7;2) bottom side view, cavity of the display for optional washer；3) illustrative
8 feature of top surface hole of microfluidic device 5 is in position and corresponding with the foot on modularizing member in shape；4) microfluidic device
5 bottom side view, which show cavity features 9;5) view at the back side 3/4 of microfluidic device 5, wherein foot 7 are from swab
Port 1 passes through alignment hole and cavity feature；6) low profile view shows that foot 7 is sticked to except the bottom surface of microfluidic device 5；7)
Foot 7 is melted in cavity 9；8) finally it is thermally compressed 3/4 view of the top surface of part (these parts are shown as transparent).
In certain embodiments, the modular part (such as swab port) of thermo-compression bonding includes sufficiently long feature, so as to
Across miniflow body portion.These are referred to as the foot 7 of the part, and can have any shape/size (such as cylindrical, side
Shape stake, triangular pile etc.).In certain embodiments, modular part includes at least one 7 (example of foot for thermo-compression bonding
Such as 1,2,3,4 Ge Huogengge foots).In certain embodiments, component includes 3 or 4 foots 7, to connect below
Tie the equal distribution of power.Foot can be made of any suitable material (such as cold melt plastics, such as polyacrylic acid or polyphenyl
Ethylene).Foot may also include feather fractures and other feature, to contribute to the part to be buckled on suitable position.
In certain embodiments, analysis component (such as microfluidic device 5), which blocks, has corresponding hole 8, the top with card
The shape of foot on face matches, and include microfluidic device 5 bottom surface on bigger cavity feature 9.Hole and foot can
Include the alignment characteristics for making foot/hole positioning, and may also include the use for having key feature so that the part can only pass through
The position of foot, or unidirectionally connection carried out by the different feet shape in space (such as the part of three foots of band can have
The triangular pile of barrette positioned at bottom left, the arc-shaped pile positioned at bottom right and rest position).In foot 7 and micro-
After fluid means 5 is in contact (with the foot across hole), these devices are optionally clamped together.Then make foot
It is melted in backside cavity, and allows cooling in place.Brought up device is shown in the panel 8 of Fig. 2.
Optionally, washer can be placed between modularizing member and microfluidic card, to enhance fluidity sealing.In certain realities
It applies in example, the backside cavity 9 on microfluidic device 5 has sufficiently large volume, to adapt to foot's volume of fusing.Foot
After cooling, clamping force or compressing force can be removed if necessary, and washer it is in place or under conditions of without washer by module
Change component to be attached on microfluidic device 5.
Thermo-compression bonding completed using any suitable method, including but not limited to soldering iron, hot plate or be suitable for fusing wipe
Other devices of the foot of subport.
Once having removed clamping pressure, thermo-compression bonding part is just permanently attached on microfluidic card.If desired, passing through
Refuse foot simultaneously pulls out swab port so as to remove the part.Alternatively, any desired retention mechanism/structure can be used
Part.
The illustrative diagram for the method for making thermo-compression bonding part be aligned by key is shown in figure 3.It is one or more or
The combination of these methods can be used for the part being thermally compressed into miniflow body portion.Fig. 3, which is shown, can be used for making the component to orient
The various sizes and shape of foot 7 on to microfluidic card 5.Fig. 3 shows panel 1) multiple feet shapes are utilized, with
By being keyed feature.Microfluidic card has corresponding hole, is matched with these shapes so that the part is only a side
It is aligned upwards with card；2) the different sizes of same characteristic features type are used.3) asymmetrical foot is used；4) using movement installation
The structure of type.
In another embodiment, it substitutes using personalized foot, the major part on the periphery of the part is designed to pass through
Miniflow body portion (leaves the part for microchannel).
C) enclosure system starts from individual member, but constitutes entirety with port later, is used as washer and lid.
In certain embodiments, integral piece washer/enclosure system is used for swab port.This part is shown in Fig. 4.
Fig. 4 shows the top view and bottom view (1 and 2) of integral piece washer/lid 10.In certain embodiments, washer/lid 10 is by flexible material
Material such as silicon rubber is made, and foldable/be bent to appropriate position, to be assemblied in around the main body of swab port.
It is the edge outstanding 11 in the gasket areas of the device that Fig. 4, which also shows (lower panel),.Convex ridge is designed as making pressure
Power is concentrated, and swab port is sealed on card.Lid/housing region 3 has cavity 12 to allow to have on the swab to fracture
Head space and convex ridge 13, laterally periphery extends convex ridge 13, to be assembled in the areola inside swab retainer.
Fig. 5 shows how washer/housing device 10 matches with swab port 1.It is hot pressed until two and is connected to miniflow
When on body card, they are press-fitted/keep together by friction.Following picture, which highlights, to be shown from the bottom of sub-component
The convex ridge that protrusion is come.These convex ridges are pressed against during thermo-compression bonding on microfluidic card, to form preferable sealing.Once swab
Port, which is hot pressed, to be connected on microfluidic card, and washer is just captured, and because lid/housing region is also identical with lid/shell
Device, so lid is also permanently attached on microfluidic card/swab port assembly.This provides the lid that will not be lost on device
Additional benefit.
In other embodiments, swab port is connected to using such as solvent linking method, adhesive or double faced adhesive tape
On microfluidic device.
D) main body-buckles port/return port concept for the swab of mixing/recycling outside plane.
In certain embodiments, present disclose provides features to allow user that swab is placed on inside swab port and is rolled over
The bar part of disconnected otiose swab, while completely including the part of swab worked.In certain embodiments, the disclosure carries
The methodology for allowing liquid to flow back/pass through swab in a controlled manner is supplied.
Fig. 6 shows that swab 14, which is placed into swab, to be inserted into component 4, then fractures the mobile jib of swab, makes to include sample
This swab portion is retained in the inside of swab port.Help to fracture and is characterized in neck 15 shown in fig. 7.It is inserted in swab
The bottom for entering component 4 provides the cavity 16 of the bigger for swab.The cavity is tapered towards top, wherein its is bigger
In the bar portion of swab, to form bottleneck.This feature allows swab to be pushed with moderate active force, but still retains and wipe
Son, except non-user specially pulls it.The neck 15 also serves as the point for concentrating the active force on bar.When user is to entire swab
When using cross force, swab fractures in neck, thus useful swab portion is retained in port, and removes stock, otherwise
It will be difficult to be sealed and/or be loaded into instrument.
Fig. 7 shows the return port 17 close to swab port.Flow path (the stream of sample fluid is shown in the figure 7
Body path direction is also possible to be reversed).Return port 17 is the mullion of sky, is used as swab bottom being connected at the top of swab
On conduit or channel.Then fluid can be pumped to above by return port, at return port, by horizontal channel across
More swab port flows to the top of swab.Then the liquid of the volume is drawn on swab surface, and (liquid oozes across swab
Inside saturating swab), then under on microfluidic card, liquid is pushed back to above by return port here.The process quilt
Repeated several times (or being equivalent to several column volumes), and significantly enhance liquid and any biological material on swab and swab
The interaction of material.For example, in certain embodiments, which helps to remove cellular material from swab, and enhance thin
Cellular lysis, it is assumed that liquid solution is dissolving buffer.It also contributes to medical jurisprudence swab and clinical swab (nose/larynx/wound
Mouthful) on biomaterial (such as nucleic acid, propylhomoserin, fat, oil, metabolin etc.) the rate of recovery.Embodiment of the disclosure also exists
Purposes, such as the recycling of enhancing explosion residue object are had found in abiotic application.Return port need not use lid/shell system
System, but they can be used together (such as reducing the risk of leakage and cross contamination).In addition, the device is not necessarily used for its time
Flow the swab of function.For example, in certain embodiments, which is loaded with sample (such as whole blood), and blood is slow with dissolving
Electuary flows back.Swab port is not limited to specific sample or return port size.In certain embodiments, swab end
Mouth keeps the liquid of μ L in total ~ 300 under conditions of swab is not present；However can specifically contemplated smaller and bigger volume
Fig. 8 shows the additional illustration of the liquid recycle by return port.
II. the device in use
Fig. 8 shows the schematic diagram when device of the embodiment of the present invention uses.
The panel on the ultra-Right side of Fig. 8 demonstrates the DNA concentration of acquisition between 15-90ng/uL, is greater than or equal to tradition
The swab concentration for the treatment of of bench scale.
During studying embodiment described here in performed experimentation, liquid is recycled by swab port.
Swab is placed in swab port, and most of swab bar portions are broken off and (pumping of swab are made to be partially left in column).Coloring
Food is pumped to through microfluid on swab and return port, is fluid separation to demonstrate column, other than the grooving at top, and
And pass through microfluidic flow in bottom.Microfluidic device design from liquid storage groove for pumping to swab and return port simultaneously
It is useful.
A it) chemically examines
In certain embodiments, device described here, system and external member have found purposes in analyzing and detecting chemical examination.
Swab port and microfluidic device described here in biological (such as nucleic acid, propylhomoserin, fat, lipid, metabolin, small molecule) and
Purposes is had found in the detection and analysis of chemistry (such as environment or war chemistry) analyte.
Microfluidic device has found purposes in various chemical examinations, including but not limited to nucleic acid amplification, mix chemical examination, be immune
Measurement, biochemical assays etc..
In certain embodiments, the analyte of amplification is further detected using suitable technology.For example, certain
In embodiment, the base content of amplified production is determined by the molecular mass detected, to identify nucleic acid analyte.In these realities
Apply in example, base content usually with the corresponding template nucleic acid in the identity characteristic of biological source, genotype or given sample
Other attributes are associated.The database of base content and other information useful during these are also typically contained in these and are
In system.Suitable software and related aspect, such as determining base content from the molecular mass of detection and for executing
The other aspects of base content analysis commercially can be from (California, USA Carlsbad) Ibis bioscience public affairs
Department obtains.
Described in various patents and patent applications be optionally suitable for for system described here use based on point
The detection method of protonatomic mass and the specific embodiments of other aspects comprising such as U.S. Patent number 7,108,974; 7,
217,510; 7,226,739; 7,255,992;7,312,036 and 7,339,051 and US Patent Publication Number 2003/
0027135; 2003/0167133; 2003/0167134; 2003/0175695; 2003/0175696; 2003/
0175697; 152003/0187588; 2003/0187593; 2003/0190605; 2003/0225529; 2003/
0228571; 2004/0110169; 2004/0117129; 2004/0121309; 2004/0121310; 2004/
0121311; 2004/0121312; 2004/0121313; 2004/0121314; 2004/0121315; 2004/
0121329; 2004/0121335; 2004/0121340; 2004/0122598; 2004/0122857; 2004/
0161770; 2004/0185438; 2004/0202997; 2004/0209260; 2004/0219517; 2004/
0253583; 2004/0253619; 2005/0027459; 2005/0123952; 2005/01301962005/0142581;
2005/0164215; 2005/0266397; 2005/0270191; 2006/0014154; 2006/0121520; 2006/
0205040; 2006/0240412; 2006/0259249; 2006/0275749; 2006/0275788; 2007/
0087336; 2007/0087337; 2007/0087338; 2007/0087339; 2007/0087340; 2007/
0087341; 2007/0184434; 2007/0218467; 2007/0218467; 2007/0218489; 2007/
0224614; 2007/0238116; 2007/0243544; 2007/0248969; WO2002/070664; WO2003/
001976; WO2003/100035; WO2004/009849; WO2004/052175; WO2004/053076; WO2004/
053141; WO2004/053164; WO2004/060278; WO2004/093644; WO2004/101809; WO2004/
111187; WO2005/023083; WO2005/023986; WO2005/024046; WO2005/033271; WO2005/
036369; WO2005/086634; WO2005/089128; WO2005/091971; WO2005/092059; WO2005/
094421; WO2005/098047; WO2005/116263; WO2005/117270; WO2006/019784; WO2006/
034294; WO2006/071241; WO2006/094238; WO2006/116127; WO2006/135400; WO2007/
014045; WO2007/047778; WO2007/086904;And WO2007/100397;WO2007/118222, they pass through
It quotes and is fully incorporated in herein.
Illustratively the analysis method based on molecular mass and the other aspects for system described here are with hereafter
Be described in offering, for example, Ecker et al. (2005) " microorganism Rosetta stone database:The whole world and emerging infectious disease
Microorganism and bio-terror threats reagent compilation " BMC microbiologies 5 (1):19；" the Ibis T5000 of Ecker et al. (2006)
Omnipotent biosensor:Platform for pathogenic bacteria identification and the automation of deformation type " JALA6 (11): 341-351；
" the identification of the acinetobacter carried out by multidigit PCR and measuring method of mass spectrum and the Bao Man not lever of Ecker et al. (2006)
The Genotyping of bacterium " clinical microbiology magazine 44 (8):2921-32；Ecker's et al. (2005) " monitors for epidemic disease
Respiratory tract pathogenic bacteria quickly identify and answer Variational-Type " National Academy of Sciences 102 (22):8012-7；Hannis et al.
(2008) " the high-resolution genetic typing of the campylobacter carried out by using PCR and high-throughput measuring method of mass spectrum " is faced
Bed JOURNAL OF MICROBIOLOGY 46 (4)：1220-5；Blyn's et al. (2008) " is carried out using the ionization measuring method of mass spectrum after PCR
Adenovirus it is quick detection and molecule serotype " clinical microbiology magazine 46 (2)：644-51；Sampath et al.
(2007) " passing through the global monitoring for the urgent influenza virus genetic typing that measuring method of mass spectrum carries out " PLoS One2
(5):e489；" the urgent infectious agents carried out using PCR and ionization measuring method of mass spectrum of Sampath et al. (2007)
Quick identification " NY Academy of Sciences institute report 1102:109-20；Hall's et al. (2005) " utilizes ionization measuring method of mass spectrum
The base content of the human mitochondrial DNA of progress is analyzed:A kind of novel tools for human bioequivalence and differentiation " analytical biochemistry
344(l):53-69；Hofstadler's et al. (2003) " carries out PCR product purification by ionization measuring method of mass spectrum and takes off
Highly effective and automation the method for salt analysis " analytical biochemistry 316:50-57；Hofstadler's et al. (2006)
" ion filter of selectivity is carried out by digital threshold:A kind of chemistry unlocked complicated ESI- mass spectrums and eliminate low molecular weight is made an uproar
The method of acoustical signal " analytical chemistry 78 (2):372-378；And " the TIGER of Hofstadler et al. (2005):Omnipotent biology
Sensor " world mass spectroscopy association 242 (1):23-41, they are incorporated by reference herein.
Other than above-mentioned molecular mass and base content are analyzed, substantially any other nucleic acid amplification technologies techniques also may be used
The system that selection of land is suitable for the present invention uses.The other examples purposes and other aspects of present system include immunoassays,
Cell culture, based on cell assays, compound library screening and chemical synthesis etc..It is answered for many these in present system
To and other illustrative applications be also described in the following documents, such as molecular biology I, II and III volumes 1997
Current protocol in (F. M. Ausubel editions)；The practice guideline of Perbal molecular clonings in 1984；In enzyme agent
Series methods (academic press)；Sambrook et al. molecular cloning experiment handbooks in 2001, the 3rd edition, cold spring harbor laboratory
Publishing house, York Cold Spring Harbor；Oligonucleotide synthesizes 1984 (M.L. Gait editions)；Nucleic acid hybridization 1985 (Hames and
Higgins)；Transcription and translation 1984 (Hames and Higgins editions)；Animal cell culture 1986 (R. I. Freshney editions)；
Berger and Kimmel, molecule clone technology guide, enzyme agent method volume 152, academic press, California
San Diego city (Berger), DNA vegetative propagations：Put into practice method, I and II volumes, 1985 (D. N. Glover editions)；Fixed cell
And enzyme, 1986 (IRL publishing houses)；For the gene delivery vector of mammalian cell, 1987 (J. H. Miller and M. P.
Calos editions, cold spring harbor laboratory)；And volume 154 and volume 155 of the method in enzyme agent (is respectively Wu ＆
Grossman and Wu editions), they are incorporated by reference herein.
B) external member and system
In certain embodiments, swab port and relevant component are provided in the form of external member.In order to for example, at certain
In a little embodiments, external member only includes swab port, and in the embodiment of other examples, external member further includes lid, washer, miniflow
Body device etc..Material included in given external member often relies on the expected purpose of device (such as nucleic acid or albumen
Matter purification technical process, for cell culture process process or screening application, for tint or printing application, be used for chemical synthesis
Technical process etc.).Therefore, the unrestricted examples of materials being optionally included in external member be magnetic-responsive particulate (such as
Magnetic response pearl etc.), water, solvent, buffer, reagent, cell culture medium, cell, japanning, ink, biopolymer (such as
Nucleic acid, polypeptide etc.), solid support (such as controlled pore glass (CPG) etc.) and homologue.External member also typically includes
Utilize the guide of device and system described here.In addition, external member also generally includes the packet to contain device and/or guide
Dress.
External member receives order from the user commonly used in response.Order is received by various mechanisms, including for example logical
The personal appearance for crossing user or its agency, by postal or other services of sending (such as common carrier), by telephone communication,
Pass through E-mail communication or another electronic media or any other suitable method.In addition, external member is usually by any suitable
Method supply or be supplied to user's (such as exchanging form of payment), including by user or its procuratorial personal appearance, lead to
Cross postal or other services of sending, such as common carrier etc..
In certain embodiments, swab port and microfluidic device are provided as a part for system.In certain implementations
In example, system includes swab port (such as being provided in the form of external member) and microfluidic device and other assay devices.At certain
In a little embodiments, system further includes sample operations component and automation chemical examination component.
Sample operations component and/or other component of a system are typically coupled to suitable program processor, computer, number dress
Set or other logic devices or massaging device on (such as include analog-to-digital conversion or digital analog converter as needed), be used for basis
Preprogrammed instruction or user input instruction (such as the addition of reagent, the transmission on reagent to additional member, have it is to be passed
Fluid volume etc.) operation of instructing these instruments, receive data and information from these instruments, and to user's translation,
Operate and report the information.
Controller or computer optionally include monitor, are that cathode-ray tube (" CRT ") display, tablet are aobvious often
Show device (such as radioactivity array liquid crystal display, liquid crystal display etc.) or other displays.Computer circuits are placed often
In box comprising many IC chips, such as microprocessor, memory, interface circuit etc..Box also optionally wraps
Include hard disk drive, floppy disk, large capacity movable type driver, such as erasable CD-ROM and other common peripheral hardwares
Element.Input unit such as keyboard or mouse optionally provide for input from the user.
Computer generally includes, for receiving user instructions suitable software, the shape of one group of parameter to be inputted using user
Formula, such as in the gui, or using the form of preprogrammed instruction, such as the pre- of a variety of different specific operations
The instruction of first sequencing.
Then software converts these instructions into suitable language, the operation for instructing one or more controllers, from
And the operation needed for executing.Then computer is received from for example including the data of sensors/detectors in systems, and turn over
It translates the data or is provided with the format that user understands, or control is further initialized using the data according to programming
Device instructs.
Computer may be such as PC (Intel x86 or DOS, OS2, Windows of Pentium chip-compatibilityTM、
Windows NTTM、Windows XPTM、Windows VistaTM, machine, Macintosh based on LinuxTM, Power PC or
Machine (such as SUN based on UNIXTMWork station) or those of skill in the art known to other public commercial obtain
The computer arrived.Standard table top application such as Word (such as Microsoft WordTM, Corel Word
PerfectTM) and database software (i.e. spreadsheet, such as Microsoft ExcelTM、Corel Quattro ProTM
Or database program, such as Microsoft AccessTMOr ParadoxTM) it is suitably adapted for the present invention.For executing such as sample
The software of operation, chemical examination detection and data deconvolution optionally designs language by those of skill in the art using standardization program
Speech, such as Visual basic, C, C++, Fortran, Basic, Java etc. are constructed.
In certain embodiments, system includes detection means, is configured to from given technique, such as in microfluidic device
In detect one or more detectable signals or parameter in performed chemical examination.In certain embodiments, system configuration is inspection
Detectable signal or parameter are surveyed, the upstream and/or downstream of given chemical examination are located at.It is optionally used in such systems
Suitable signal detector have detected for example pH value, temperature, pressure, density, salinity, conductivity, liquid level, radioactivity, shine
Property, fluorescence, phosphorescent, molecular mass, emissivity, transmittance, absorbance etc..In certain embodiments, Sensor monitoring
Multiple signals, it is corresponding with " real-time " result in position.The example of detector or sensor includes PMT, CCD, enhancing
CCD, photodiode, avalanche mode photodiodes, optical sensor, scanner detector etc..The biography of these and other type
Sensor is optionally incorporated into system described here.Detector holds optionally with respect to assay device or workbench, sample
Device or other chemical examination components and move or assay device or workbench, sample container or other chemical examination components are relative to detection
Device and move.Optionally, system includes multiple detectors.
Detector is optionally included or is operatively coupled on computer, i.e., it is with system software, for that will detect
Device signal message is converted into result of laboratory test information etc..For example, detector optionally exists with separate unit, or and controller
It is integrated into single instrument.These functions are integrated into the connection that individual unit promotes these instruments and computer, pass through permission
Using several or even single communication port, to transmit information between the component of a system.In the instrument point of such as Skoog et al.
Analyse principle the 6th edition, the analytical instrument of Brooks Cole (2006) and Currell：Performance characteristic and quality, John Wiley ＆
Sons companies further describe the detection means being optionally included in present system in (2000), they are by reference
It is incorporated herein.
The system further includes optionally that at least one robot is mobile or clamping component, structure are used in workbench or are
It is clamped between the component of system and/or between workbench or system and other positions (such as other workbench etc.) and mobile
Swab port or microfluidic device or other components.Various available robotic components (mechanical arm, moveable platform etc.)
It can be used for or be used for by improvement these systems, these robotic components typically operatively connect on the controller, control
Make their movement and other functions.
Suitable linear movement component, motor and motor driver can usually be obtained from many different commercial suppliers,
Including, such as Techno-Isel linear motion systems company (the new Hyde Park in USA New York), (U.S. of NC servos scientific ＆ technical corporation
State of Michigan Westland city), Enprotech automation services (Michigan, USA Ann Arbor city), the An Chuan motors U.S. it is public
It is public to take charge of (U.S. is according to the sharp Northey state cities Wo Jigen), ISL products international corporation (USA New York oersted city), AMK drivings and control
Take charge of (Richmond, VA, USA city), Aerotech companies (city of Pennsylvania, United States Pittsburgh), HD system house
(USA New York Hauppauge city) etc.." motor and driver " ISA's (2002) and Hendershot of such as Polka et al.
It is described in " design of brushless permanent magnet motor " (Magna Institute of Physics Publishing (1994)) relevant with motor and motor driver
Subsidiary details, they are incorporated herein by reference.It is submitted within 16th in September in 2008 in such as Hofstadler et al.
No. DIBIS-0116US.L of entitled " minitype plate operating system and relevant computer program product and method " agency
Minitype plate operating member is also illustrated in people's abstract, they are fully incorporated in herein by reference.
Although the present invention of front, this field have been described in certain details for purpose that is clear and understanding
In technical staff should be understood that in the true scope for not departing from the present invention by reading this specification, can be in form and thin
Various change is carried out on section.For example, above-mentioned all technology and device various can combine to use.Cited by the application
All publications, patent, patent application and/or other documents are fully incorporated in herein by reference, to reach identical
Whole purposes of degree, as each individual publication, patent, patent application and/or other documents are by individually through drawing
With and be incorporated herein, to reach all purposes.
Claims (25)
1. a kind of swab port device comprising：
At least one agent structure comprising one or more surfaces, one or more of surfaces, which limit, to be had in size side
Face is set as receiving the first swab port cavity of the upper part and low portion of sample collection swab, and having will be described
The bottom of swab in first swab port cavity is connected to the top of the swab in the first swab port cavity
Second return port cavity of upper part and low portion, wherein the upper part of the first swab port cavity with
The upper part communication of the second return port cavity, wherein the fluid communication includes channel, and it is described
The low portion communication of the low portion of first swab port cavity and the second return port cavity,
The wherein described fluid communication includes microfluidic card；
Lid, is configured to seal first swab in the first swab port cavity and the second return port cavity
Port cavity and the second return port cavity；
Washer is located under the first swab port cavity and the second return port cavity；And
Flexible arm connects the lid and the washer, and wherein single type casing part includes the lid, the washer and described
Flexible arm.
2. the apparatus according to claim 1, which is characterized in that described device further includes at least one of sample collection swab
Point.
3. the apparatus according to claim 1, which is characterized in that the first swab port cavity and second reflux end
Mouth cavity keeps being in fluid communication.
4. the apparatus according to claim 1, which is characterized in that the first swab port cavity includes about 300 μ L
Volume capacity.
5. the apparatus according to claim 1, which is characterized in that the agent structure further includes multiple protrusions.
6. device according to claim 5, which is characterized in that the protrusion includes that different sizes or the multiple of shape dash forward
It rises.
7. device according to claim 5, which is characterized in that the protrusion is configured to make in described device and analytical equipment
Hole alignment.
8. the apparatus according to claim 1, which is characterized in that the lid includes lid containment member and washer member.
9. the apparatus according to claim 1, which is characterized in that the first swab port cavity includes neck.
10. the apparatus according to claim 1, which is characterized in that the lid is integrated into the swab port device.
11. the apparatus according to claim 1, which is characterized in that the first swab port cavity has inner surface, described
Inner surface includes one or more protrusions, is configured to contribute to remove material from the swab of insertion.
12. a kind of assay system comprising：
Device according to any one of claim 1 to 11；With
The chemical examination component being connected to described device.
13. assay system according to claim 12, which is characterized in that the agent structure of described device further includes more
The protrusion of a protrusion, the agent structure is inserted in the hole in the chemical examination component.
14. assay system according to claim 13, which is characterized in that the protrusion is thermally sealed on the chemical examination component
On.
15. assay system according to claim 12, which is characterized in that the chemical examination component is microfluidic device.
16. assay system according to claim 12, which is characterized in that the assay system further includes sample analysis structure
Part is operatively coupled on the chemical examination component.
17. a kind of assay method comprising：
A) swab port device is made to be in contact with chemical examination component, the swab port device includes i) at least one agent structure,
It includes one or more surfaces, and one or more of surfaces define the first swab with upper part and low portion
Port cavity and with by the bottom of the swab in the first swab port cavity be connected to first swab port sky
The upper part at the top of the swab in chamber and the second return port cavity of low portion, wherein first swab end
The upper part communication of the upper part and the second return port cavity of mouth cavity, wherein the stream
Body connection includes channel, and the institute of the low portion of the first swab port cavity and the second return port cavity
Low portion communication is stated, wherein the fluid communication includes microfluidic card, and lid, it is configured to wipe described first
The first swab port cavity and second return port are sealed on subport cavity and the second return port cavity
Cavity, washer, under the first swab port cavity and the second return port cavity and flexible arm, connection
The lid and the washer, wherein single type casing part include the lid, the washer and the flexible arm；And ii) from institute
The outstanding multiple protrusions in the bottom of agent structure are stated, and the chemical examination component includes hole, is arranged to connect in terms of size
Receive the protrusion；And
B) described device is sealed to the chemical examination structure by the heat for making the protrusion be melted on the chemical examination component by application
On part.
18. a kind of assay method comprising：
The assay system of claim 12 is set to be in contact with the swab including sample；With
The liquid being included in the second return port cavity is set to be recycled by the first swab port cavity.
19. assay method according to claim 18, which is characterized in that further comprising the steps of：Fracture the swab
End so that the part comprising the sample of the swab is retained in the first swab port cavity of described device.
20. assay method according to claim 18, which is characterized in that the sample is delivered to the liquid.
21. assay method according to claim 20, which is characterized in that further include making the liquid and the chemical examination component
The step of contact.
22. assay method according to claim 21, which is characterized in that further include the analyte in the identification sample
Step.
23. assay method according to claim 22, which is characterized in that the analyte is selected from by nucleic acid, propylhomoserin, fat
The group of matter, metabolin and chemicals composition.
24. the purposes of the device according to any one of claim 1-11.
25. the device according to any one of claim 1-11 is used to acquire and analyze the use of the sample from swab
On the way.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261705967P | 2012-09-26 | 2012-09-26 | |
| US61/705967 | 2012-09-26 | ||
| PCT/US2013/061921 WO2014052590A1 (en) | 2012-09-26 | 2013-09-26 | Swab interface for a microfluidic device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104937390A CN104937390A (en) | 2015-09-23 |
| CN104937390B true CN104937390B (en) | 2018-09-21 |
Family
ID=50388961
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380061295.7A Expired - Fee Related CN104937390B (en) | 2012-09-26 | 2013-09-26 | swab interface for microfluidic device |
Country Status (4)
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|---|---|
| US (1) | US20150241319A1 (en) |
| EP (1) | EP2901129A4 (en) |
| CN (1) | CN104937390B (en) |
| WO (1) | WO2014052590A1 (en) |
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| US10946376B2 (en) * | 2013-07-05 | 2021-03-16 | Thinxxs Microtechnology Ag | Carrier element for introducing a dry substance into a flow cell |
| EP2821138B2 (en) * | 2013-07-05 | 2022-02-09 | Thinxxs Microtechnology Ag | Flow cell with integrated dry substance |
| JP2017531807A (en) * | 2014-10-23 | 2017-10-26 | アイビス バイオサイエンシズ インコーポレイティッド | Swab port for microfluidic devices |
| KR102385794B1 (en) | 2015-04-07 | 2022-04-11 | 셀 아이디 피티이 엘티디 | A dc heater |
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| CA3177331A1 (en) * | 2020-04-29 | 2021-11-04 | Livia Schiavinato Eberlin | Collecting and analyzing swab samples |
| CN112472140A (en) * | 2020-05-11 | 2021-03-12 | 中山大学达安基因股份有限公司 | Storage tube for sampling swab |
| CN114397168B (en) * | 2022-03-28 | 2022-06-17 | 江西汉氏医学发展有限公司 | Internet-based body fluid balance intelligent health detection equipment and detection method |
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Also Published As
| Publication number | Publication date |
|---|---|
| EP2901129A4 (en) | 2016-11-02 |
| EP2901129A1 (en) | 2015-08-05 |
| US20150241319A1 (en) | 2015-08-27 |
| WO2014052590A1 (en) | 2014-04-03 |
| CN104937390A (en) | 2015-09-23 |
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