CN104931685B - A kind of based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen - Google Patents
A kind of based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen Download PDFInfo
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- CN104931685B CN104931685B CN201510312077.7A CN201510312077A CN104931685B CN 104931685 B CN104931685 B CN 104931685B CN 201510312077 A CN201510312077 A CN 201510312077A CN 104931685 B CN104931685 B CN 104931685B
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- Prior art keywords
- antibody
- recombinant antigen
- label
- detection method
- label recombinant
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
Abstract
The invention provides a kind of based on carrying the electrochemiluminescent immunoassay detection method of His label recombinant antigen, based on carrying, the recombiant protein of His label is coated luminous microsphere as known antigens, anti-His antibody, biotin labeling second antibody, the photosensitive microsphere of marked by streptavidin collectively constitute detectable.The present invention is to carry the recombiant protein of His label as known antigens, and most important characteristics is that this known antigens is placed in liquid phase three dimensions, there is essential distinction with conventional art immobilization antigen.And this change effectively overcomes the technical problem that conventional antibodies detection method is brought because of immobilization process.Additionally, utilize homogeneous luminescent immunoassay system based on the transmission of active oxygen energy, there is higher analysis sensitivity and precision.
Description
Technical field
The invention belongs to the antibody test technical field of Medical Laboratory Specialty, mainly include anti-allergen antibodies,
Pathogen antigen and autoantibody, especially relate to a kind of based on the luminescence carrying His label recombinant antigen
Immunologic detection method.
Background technology
Immunochemistry (antigen-antibody reaction) technology is the important method of detection specific antibody, i.e. adopts
With the unknown antibody format of known antigens detection.Elisa (ELISA) indirect method is usually used in
Detect above-mentioned antibody, it is possible to use enzyme connection dot blot assays (dot-ELISA) with " linear mode "
It is coated multiple related antigen, to realize detecting Multiple Antibodies (such as antinuclear antibodies spectrum, HIV sense simultaneously simultaneously
Dye validation test etc.).The ultimate principle of above two method is as follows: be coated solid phase carrier by known antigens
(polystyrene micropore plate or nitrocellulose filter)；Adding serum temperature to be checked bath, antibody to be checked is anti-with known
Former combination forms immune complex (or antibody to be checked is captured by solid phase antigen)；Add enzymic-labelled antibody more also
Temperature bath, traget antibody and antibodies to be checked, uncombined antibody is removed by " washing "；Finally add the end
Thing develops the color, and reads absorbance (A) by microplate reader or directly observe film surface speckle after terminating enzymatic reaction
Point result of determination.
Actual application finding, said method exists the defect of some technical elements, and these defective effects are examined
Survey result accuracy or bring some difficulty to commercially producing, being mainly manifested in following aspect:
It is that isolation technics is commonly used in non-homogeneous enzyme immunoassay analysis that one, immobilization problem solid phase adsorption separate,
Its principle is at solid phase material surface (polystyrene micropore or nitrocellulose membrane) by antigen solid phase, and treats
Antigen-antibody-hrp-antibody complex to be checked that inspection antibody and enzyme labelled antibody are formed is present in solid phase material
Surface, and free label is distributed in liquid phase.Topple over liquid solution and can easily be gone by " washing "
Except enzymic-labelled antibody interference measurement, free.It is well known, however, that antigen (wraps after being connected with solid phase material
Quilt), it is distributed in the antigen molecule on solid phase material surface, its structure no longer has the native conformation in liquid phase.
The change of this molecular conformation will affect known antigens and antibodies to be checked；Meanwhile, solid phase antigen position
In solid phase material surface, because space steric effect can have a strong impact on and antibodies to be checked.Additionally, when bag
During by peptide molecule that antigen is molecular weight, often can lose its biological activity because of the process of being coated, or tie
Close antibody ability to be remarkably decreased.
Two, envelope antigen purity and multiple antigen combination problem solid-phase coating be by antigen coated
In solid phase plane, microwell plate or nitrocellulose membrane be coated limited area, require relatively for coated antigen
High purity, and antigen purification process can increase the production cost of detection kit.Meanwhile, no matter allergy
Former sIgE antibody, or pathogen antigen or autoantibody, it is generally required to multiple protein is as known anti-
Former, and the above-mentioned solid phase mode of being coated is not appropriate for multiple antigenic and is coated simultaneously.Multiple antigenic is coated and relates to
And between component ratio and sterically hindered cause interfere.
Three, preparation process and use process complexity no matter elisa or enzyme connection speckle
Blot analysis, antigen immobilization process is the key link that diagnostic reagent produces, antigen coated-to close-dry
The process such as dry is more complicated, it is achieved standardization needs S.O.P. strict implement.In terms of user perspective,
The use of above two detectable is the most more complicated, and clinical samples needs dilution, twice incubation and repeatedly
Washing process takes long enough, and traget antibody (enzyme) is affected by many factors etc., is also unfavorable for behaviour
The use of author.
For the defect of above-mentioned technical elements, this technological invention relates to a kind of based on having carried His label
Know recombinant antigen, use active oxygen ability transmission homogeneous luminescent immunoassay principle, set up a kind of hair
Light immunoassay system, detects for specific IgE, pathogen antigen and autoantibody.
Summary of the invention
In view of this, it is contemplated that propose a kind of based on the electrochemiluminescent immunoassay carrying His label recombinant antigen
Detection method, uses active oxygen energy transmission homogeneous luminescent immunoassay principle, is put by known recombinant antigen
In liquid phase three dimensions, there is essential distinction with conventional art immobilization antigen, the present invention sets up a kind of new
Type luminescence immunoassay system, detects for specific IgE, pathogen antigen and autoantibody, has
Higher analysis sensitivity and precision, and preparation technology is simple, easy to operate.
For reaching above-mentioned purpose, the technical scheme of the invention is achieved in that
A kind of comprise the steps based on carrying the electrochemiluminescent immunoassay detection method of His label recombinant antigen,
1) His label recombinant antigen is carried in acquisition；
2) by serum to be checked, to carry His label recombinant antigen solution, the coated luminescence of His antibody micro-
Ball solution fully mixes, and in 37 DEG C of temperature baths 30～45min；
3), after temperature bath, through high speed centrifugation 10～15 minutes, supernatant is abandoned；
4) biotin labeled second antibody solution and the coated photosensitive microspheres solution of Streptavidin are added,
Fully mixing, 37 DEG C of temperature baths 15～30min；
5) use up and excite luminometer to detect optical signal under 615nm.
The described His label recombinant antigen that carries, upstream (left end) carries His label, and downstream is to be checked
The recombiant protein (known antigens) that antibody is targeted, recombiant protein is with different epitopes (as shown
Containing 3 kinds of epitopes), this recombiant protein can capture antibody to be checked.
Described biotin labeled second antibody, is marked with biotin molecule, because antibody to be checked is behaved immune
Globulin, can use rabbit anti human IgE (being used for detecting IgE antibody-like) or rabbit anti-human igg (to be used for examining
Survey IgG antibody-like).
Described Streptavidin is coated photosensitive microsphere, and photosensitive microsphere is combined with biotin, carries photosensitive simultaneously
Material, can produce active oxygen species with laser when irradiating.
The coated luminous microsphere of described anti-His albumen, luminous microsphere and His protein binding, accept simultaneously
Active oxygen energy, can induce optical signal to produce.
Preferably, described step 1) in, carry His label recombinant antigen and obtained by following steps,
According to antigen primary structure synthesize target DNA, and through Protocols in Molecular Biology by genes of interest with carry
The expression plasmid restructuring of His label, transfection suitable host carries out the expression of destination protein, more affine through nickel
Chromatographic column preliminary purification obtains His-recombiant protein, and this material is used for detecting antibody as known antigens.
Preferably, described step 2) in, after three kinds of material mix homogeneously, temperature bath 30～45min at 37 DEG C.
Preferably, described step 2) in, serum to be checked, carry His label recombinant antigen mixed solution,
The volume ratio of the coated luminous microsphere of His antibody is 1:1:1；And serum to be checked be with Tirs-HCl solution by
It is that 1:10～20 is diluted processing according to volume ratio, it is preferred that 1:10.
Described His label recombinant antigen mixed solution, the His antibody coated luminous microspheres solution of carrying
Depending on dilution ratio is according to the type of test antibodies.
Preferably, described step 3) in, high speed centrifugation speed is 10 × 103R/min, and high speed centrifugation
Time be 10min.
Preferably, described step 4) in, biotin labeled second antibody, Streptavidin are coated
Photosensitive microsphere is 2:4:1 with the volume ratio of serum to be checked.
Preferably, described step 4) in, described biotin labeled second antibody be rabbit anti human IgE or
One in rabbit anti-human igg.
Present invention also offers a kind of as above based on the electrochemiluminescent immunoassay carrying His label recombinant antigen
Detection method application in specific IgE detects.
Present invention provides a kind of as above based on the electrochemiluminescent immunoassay carrying His label recombinant antigen
Detection method application in pathogen IgG or IgM detect.
Invention also provides and a kind of exempt from based on the luminescence carrying His label recombinant antigen as above
The application in autoantibody IgG detects of the epidemic disease detection method.
Present invention also offers a kind of for as above based on the luminescence carrying His label recombinant antigen
The test kit of immunologic detection method, wherein test kit includes that biotin labeled second antibody, strepto-are affine
Element coated photosensitive microsphere, biotin labeling second antibody, carry His label recombinant antigen.
The present invention is coated luminescence based on carrying His label recombinant antigen as known antigens, anti-His antibody
Microsphere, biotin labeling second antibody (for the antibody of antibody isotype epi-position to be checked), strepto-is affine
The element photosensitive microsphere of labelling collectively constitutes detectable.
In the case of antibody to be checked exists, antibody to be checked forms immune complex with recombiant protein, and this is combined
Thing is combined, by antibody to be checked with biological by the coated luminous microsphere of His with the His antibody of upstream again
Element labelling second antibody combines, and is combined with the photosensitive microsphere of marked by streptavidin through biotin.By
Above-mentioned course of reaction, photosensitive microsphere and luminous microsphere are close to each other, and distance, less than 200 nanometers, meets and lives
Property oxygen transmission light-induced chemiluminescent condition, when irradiating with laser system luminescence (615nm), it was demonstrated that to be checked
Antibody exists, and according to luminous intensity, antibody to be checked is carried out quantitative analysis.Do not exist in specimen to be checked
Antibody to be checked, it is impossible to combine restructuring known antigens, also cannot induce chain reaction, photosensitive microsphere and sending out
The spacing of light microsphere is more than 200nm, it is impossible to meet active oxygen transmission light-induced chemiluminescent condition, with swashing
When light irradiates, system can not detect optical signal.
Relative to prior art, of the present invention a kind of based on the luminescence carrying His label recombinant antigen
Immunologic detection method, has the advantage that
1) Solid Free is coated process, and in liquid phase, antigen can keep native conformation, will not be to known antigens
Activity in conjunction with antibody to be checked produces significant impact；
2) known antigens being placed in liquid phase, be different from and be coated rear solid phase surface antigen, liquid phase is provided
Be " three-dimensional space ", solid phase provides " two dimensional surface space ".Three-dimensional space is several
Do not deposit " sterically hindered " effect, and can effectively reduce Ag-Ab and reach to balance required time.
3) multicomponent system detecting antibody as known antigens it is suitable for.Food allergen sIgE detects,
Because any single diet contains multiple allergic protein, this antibody-like (sIgE) is a kind of mixture.Cause of disease
Body antibody is also such.This type of antibody test needs multiple related antigen, unsuitable for solid-phase coating, and
Use designed " liquid phase reactor " pattern of this technological invention, it is possible to provide foot for multiple antigenic antibody response
Enough spaces.
4) preparation technology is simple.Anti-His antibody in the detection system of this technological invention is coated luminous micro-
Ball, biotin labeling second antibody, the photosensitive microsphere of marked by streptavidin are general detectable, suitable
For Testing index.The design of recombiant protein is also adopted by identical His label, creates bar for protein purification
Part.Meanwhile, because being not required to solid-phase coating, system is less demanding to restructuring antigen purity, simple purification.
Accompanying drawing explanation
The accompanying drawing of the part constituting the present invention is used for providing a further understanding of the present invention, the present invention's
Schematic description and description is used for explaining the present invention, is not intended that inappropriate limitation of the present invention.?
In accompanying drawing:
Fig. 1 is of the present invention a kind of based on the electrochemiluminescent immunoassay detection side carrying His label recombinant antigen
The principle schematic of method；
Description of reference numerals:
1-carries His label recombinant antigen, 2-antibody to be checked, the biotin labeled second antibody of 3-, 4-
Streptavidin is coated photosensitive microsphere, the coated luminous microsphere of 5-anti-His albumen.
Detailed description of the invention
Embodiment one
By detection milk allergy component, " beta lactoglobulin " as a example by antibody, illustrates this technological invention below
Specific implementation process.
Detectable:
(1) recombinant beta lactoglobulin, His-beta lactoglobulin
(2) biotin labeled rabbit antihuman IgE antibody
(3) the coated photosensitive microsphere of Streptavidin
(4) the coated luminous microsphere of anti-His albumen
The most biotin labeled rabbit antihuman IgE antibody, the coated photosensitive microsphere of Streptavidin, anti-
The coated luminous microsphere of His albumen is general detectable.
Serum sample:
The patients serum of doubtful milk allergy
Detection method:
(1) in detection system, the coated luminous microspheres solution of 25 μ L His antibody it is separately added into (luminous
Microsphere concentration is 20 mcg/ml), the recombinant antigen solution (protein concentration of 25 μ L band His labels
It is 10 mcg/ml), 25 μ L serum to be checked (Tris-Hcl solution 1:10 dilution), fully mix；
(2) 37 DEG C of temperature bath 30min；Through high speed centrifugation (10 × 10 after incubation3R/min) 10 minutes,
Abandon supernatant；
(3) adding 50 μ L biotin labeled second antibody solution, 100 μ l Streptavidins are coated
Photosensitive microspheres solution (concentration of photosensitive microsphere is 20 mcg/ml), fully mix；
(4) 37 DEG C of temperature bath 30min；
(5) use up excite luminometer detection optical signal (615nm).
Result interpretation:
The foregoing is only the preferred embodiment of the invention, not in order to limit present invention wound
Make, within all spirit in the invention and principle, any modification, equivalent substitution and improvement made
Deng, within should be included in the protection domain of the invention.
Claims (7)
1. one kind based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen, it is characterised in that: comprise the steps,
1) His label recombinant antigen is carried in acquisition；
2) by serum to be checked, carry the coated luminous microspheres solution of His label recombinant antigen solution, His antibody and fully mix, and in 37 DEG C of temperature baths 30～45min；
3), after temperature bath, through high speed centrifugation 10～15 minutes, supernatant is abandoned；
4) add biotin labeled second antibody solution and the coated photosensitive microspheres solution of Streptavidin, fully mix, 37 DEG C of temperature baths 15～30min；
5) use up and excite luminometer to detect optical signal under 615nm.
The most according to claim 1 based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen, it is characterized in that: described step 1) in, carry His label recombinant antigen to be obtained by following steps, target DNA is synthesized according to antigen primary structure, and through Protocols in Molecular Biology, genes of interest and the expression plasmid carrying His label are recombinated, transfection suitable host carries out the expression of destination protein, obtaining His-recombiant protein through nickel affinity chromatography post preliminary purification again, this material is used for detecting unknown antibody as known antigens.
The most according to claim 1 based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen, it is characterized in that: described step 2) in, serum to be checked, the volume ratio carrying the coated luminous microspheres solution of His label recombinant antigen solution, His antibody are 1:1:1；And serum to be checked is to use
Tris-HCl solution is that 1:10～20 is diluted processing according to volume ratio.
4. according to described in claim 1 or 3 based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen, it is characterised in that: serum to be checked be use
Tris-HCl solution is that 1:10 is diluted processing according to volume ratio.
It is the most according to claim 1 based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen, it is characterised in that: described step 3) in, high speed centrifugation speed is 10 × 103R/min, and the ultracentrifugal time be 10min.
The most according to claim 1 based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen, it is characterized in that: described step 4) in, the coated photosensitive microspheres solution of biotin labeled second antibody solution, Streptavidin is 2:4:1 with the volume ratio of serum to be checked.
7. the test kit based on the electrochemiluminescent immunoassay detection method carrying His label recombinant antigen being used for as described in any one of claim 1～6, it is characterized in that, including biotin labeled second antibody, the coated photosensitive microsphere of Streptavidin, the coated luminous microsphere of His antibody, carry His label recombinant antigen.
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| CN108351351B (en) * | 2015-11-09 | 2021-10-29 | 生物辐射实验室股份有限公司 | Assays using avidin and biotin |
| CN105486855B (en) * | 2015-11-26 | 2017-11-10 | 北京大学第一医院 | Improve indirect enzyme-linked immunosorbent assay |
| CN110823874A (en) * | 2018-08-13 | 2020-02-21 | 博阳生物科技（上海）有限公司 | Homogeneous phase chemiluminescence detection kit and application thereof |
| CN116380883B (en) * | 2023-02-13 | 2024-02-23 | 上海索昕生物科技有限公司 | Photosensitive microsphere for photoexcitation chemiluminescence detection |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101413959A (en) * | 2008-12-04 | 2009-04-22 | 江苏先声药物研究有限公司 | Immune quantitative detecting method of histilabel protein or dine fusion protein containing histidine label |
| WO2011113019A2 (en) * | 2010-03-12 | 2011-09-15 | Abbott Biotherapeutics Corp. | Ctla4 proteins and their uses |
| CN102608313A (en) * | 2012-02-27 | 2012-07-25 | 中国疾病预防控制中心病毒病预防控制所 | Anti-hepatitis A virus IgM (immunoglobulin M) AlphaLISA detection kit |
| CN102721813A (en) * | 2012-07-09 | 2012-10-10 | 沃克(天津)生物科技有限公司 | Homogeneous luminous immunoassay assay kit for prostate specific antigen and detection method therefor |
| CN102735833A (en) * | 2012-07-09 | 2012-10-17 | 沃克(天津)生物科技有限公司 | Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof |
| CN102798725A (en) * | 2012-08-13 | 2012-11-28 | 沃克(天津)生物科技有限公司 | Diagnostic kit for determination of serum total IgE, preparation method and application method |
| CN103487582A (en) * | 2013-10-11 | 2014-01-01 | 重庆出入境检验检疫局检验检疫技术中心 | Porcine reproductive and respiratory syndrome virus antibody competitive AlphaLISA detection kit and detection method thereof |
| CN103499693A (en) * | 2013-10-11 | 2014-01-08 | 重庆出入境检验检疫局检验检疫技术中心 | Competitive Alpha LISA (linked immuno sorbent assay) detection kit for classical swine fever virus (CSFV) antibody and detection method thereof |
| CN103499689A (en) * | 2013-10-11 | 2014-01-08 | 重庆出入境检验检疫局检验检疫技术中心 | Competitive Alpha LISA (linked immuno sorbent assay) detection kit for porcine circovirus (PCV) 2 antibody and detection method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8524457B2 (en) * | 2009-09-22 | 2013-09-03 | William Patterson | Method for the selection of specific affinity binders by homogeneous noncompetitive assay |
-
2015
- 2015-06-09 CN CN201510312077.7A patent/CN104931685B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101413959A (en) * | 2008-12-04 | 2009-04-22 | 江苏先声药物研究有限公司 | Immune quantitative detecting method of histilabel protein or dine fusion protein containing histidine label |
| WO2011113019A2 (en) * | 2010-03-12 | 2011-09-15 | Abbott Biotherapeutics Corp. | Ctla4 proteins and their uses |
| CN102608313A (en) * | 2012-02-27 | 2012-07-25 | 中国疾病预防控制中心病毒病预防控制所 | Anti-hepatitis A virus IgM (immunoglobulin M) AlphaLISA detection kit |
| CN102721813A (en) * | 2012-07-09 | 2012-10-10 | 沃克(天津)生物科技有限公司 | Homogeneous luminous immunoassay assay kit for prostate specific antigen and detection method therefor |
| CN102735833A (en) * | 2012-07-09 | 2012-10-17 | 沃克(天津)生物科技有限公司 | Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof |
| CN102798725A (en) * | 2012-08-13 | 2012-11-28 | 沃克(天津)生物科技有限公司 | Diagnostic kit for determination of serum total IgE, preparation method and application method |
| CN103487582A (en) * | 2013-10-11 | 2014-01-01 | 重庆出入境检验检疫局检验检疫技术中心 | Porcine reproductive and respiratory syndrome virus antibody competitive AlphaLISA detection kit and detection method thereof |
| CN103499693A (en) * | 2013-10-11 | 2014-01-08 | 重庆出入境检验检疫局检验检疫技术中心 | Competitive Alpha LISA (linked immuno sorbent assay) detection kit for classical swine fever virus (CSFV) antibody and detection method thereof |
| CN103499689A (en) * | 2013-10-11 | 2014-01-08 | 重庆出入境检验检疫局检验检疫技术中心 | Competitive Alpha LISA (linked immuno sorbent assay) detection kit for porcine circovirus (PCV) 2 antibody and detection method thereof |
Non-Patent Citations (6)
| Title |
|---|
| A Time-Resolved Fluorescence–Resonance Energy Transfer Assay for Identifying Inhibitors of Hepatitis C Virus Core Dimerization;Smitha Kota 等;《ASSAY and Drug Development Technologies》;20100228;第8卷(第1期);96-105 * |
| Chuan-Fa Chang 等.Rapid characterization of sugar-binding specifi * |
| city by in-solution proximity binding with photosensitizers.《Glycobiology》.2011,第21卷(第7期),895-902. * |
| Development of a homogeneous AlphaLISA ubiquitination assay using ubiquitin binding matrices as universal components for the detection of ubiquitinated proteins;Stephen Schneider 等;《Biochimica et Biophysica Acta》;20120405;第1823卷;2038-2045 * |
| His标签单克隆抗体的制备、鉴定及初步应用;王文礼 等;《细胞与分子免疫学杂志》;20081231;第24卷(第4期);399-400 * |
| Specific Detection of His-Tagged Proteins with Recombinant Anti-His Tag scFv-Phosphatase or scFv-Phage Fusions;P. Lindner 等;《BioTechniques》;19970131;第22卷;140-149 * |
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