CN104931492A - Detection reagent for lead content in seaweed and detection method using detection reagent - Google Patents
Detection reagent for lead content in seaweed and detection method using detection reagent Download PDFInfo
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- CN104931492A CN104931492A CN201510313250.5A CN201510313250A CN104931492A CN 104931492 A CN104931492 A CN 104931492A CN 201510313250 A CN201510313250 A CN 201510313250A CN 104931492 A CN104931492 A CN 104931492A
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Abstract
The invention provides a detection reagent for lead content in seaweed and a detection method using the detection reagent. The detection reagent comprises the following components: phenanthroline, a sodium hydroxide solution, ammonium citrate, dihydroxyacetone, petroleum ether, dimethylglyoxime, carbon disulfide, mercury chloride, isoprene glycol, ethanol and water. The detection method is as follows: carrying out supersonic extraction on a seaweed sample to obtain a seaweed sample solution for later use; taking two parts of detection reagent with the same volume, adding the seaweed sample solution to the first part of detection reagent to form a first mixed liquid, and adding deionized water with the same volume as the seaweed sample solution to the second part of detection reagent to form a second mixed liquid; taking the second mixed liquid as a reference, observing the color change of the first mixed liquid. Compared with the prior art, the detection reagent and the detection method, provided by the invention, can detect whether the lead content exceeds standard or not more quickly, the accuracy of the content detection is high, and the detection efficiency is higher.
Description
Technical field
The present invention relates to and detect reagent field, be specifically related to lead content in a kind of marine alga and detect reagent and detection method thereof.
Background technology
Along with the industrialized fast development of society, the harmful heavy metal in entered environment medium (air, water body and soil) constantly increases, if overrun, can cause the heavy metal pollution of environment.At present, the heavy metal that the most noticeable is mercury, cadmium, lead, chromium and metalloid arsenic etc. have remarkable bio-toxicity, mainly through approach such as contaminated food products, potable water and air finally by human consumption.Marine alga is one of marine product of often eating of the mankind, and heavy metal can the enrichment in marine alga due to marine pollution, and final serious threat human health, as saturnism will cause anaemia, failure of memory, insomnia, shock, infringement stomach, kidney and reproductive system etc.Research shows, a lot of regional marine alga content of beary metal of current China all severe overweights.Therefore, the detection of marine alga heavy metal is most important for the edible safety evaluating marine product.
Summary of the invention
goal of the invention:the present invention is directed to the deficiencies in the prior art, to provide in a kind of marine alga lead content to detect reagent and detection method thereof, not only there is higher accuracy, but also Pb In Exhausted Water content can have been detected more quickly.
technical scheme of the present invention:
In marine alga, lead content detects reagent, by weight, comprise following component: Phen 3 ~ 5 parts, sodium hydroxide solution 12 ~ 16 parts, ammonium citrate 3 ~ 5 parts, dihydroxyacetone 5 ~ 8 parts, sherwood oil 3 ~ 5 parts, dimethylglyoxime 1 ~ 3 part, carbon disulphide 1 ~ 3 part, mercuric chloride 1 ~ 2 part, isoprene 5 ~ 10 parts, ethanol 10 ~ 15 parts and 40 ~ 60 parts, water.
Preferably, by weight, comprise following component: Phen 4 ~ 5 parts, sodium hydroxide solution 14 ~ 15 parts, ammonium citrate 4 ~ 5 parts, dihydroxyacetone 6 ~ 7 parts, sherwood oil 4 ~ 5 parts, dimethylglyoxime 2 ~ 3 parts, carbon disulphide 1 ~ 2 part, mercuric chloride 1 part, isoprene 7 ~ 8 parts, ethanol 12 ~ 14 parts and 50 ~ 54 parts, water.
More preferably, by weight, following component is comprised: Phen 4 parts, sodium hydroxide solution 15 parts, ammonium citrate 4 parts, dihydroxyacetone 6 parts, sherwood oil 5 parts, dimethylglyoxime 2 parts, carbon disulphide 1 part, mercuric chloride 1 part, isoprene 7 parts, ethanol 13 parts and 52 parts, water.
Described concentration of sodium hydroxide solution is 15 ~ 35wt%.
In marine alga, lead content detects the detection method of reagent, comprises the following steps:
(1) seaweed sample is air-dry, grinding, crosses 100 ~ 500 mesh sieves, adds HNO
3in solution, then put into ultrasonic washing instrument, heating, ultrasonic process, take out and leave standstill cooling, centrifugal, obtain seaweed sample solution, for subsequent use;
(2) get the detection reagent of two parts of same volumes, detect in reagent to first part and add the obtained seaweed sample solution of step (1), form the first mixed liquor; Detect in reagent to second part and add the deionized water equal with seaweed sample liquor capacity, form the second mixed liquor;
(3) with the second mixed liquor for reference, observe the first mixed liquor color change, when lead content is higher than 1.0 mg/L, solution becomes blueness from aubergine, and when content of beary metal is lower than 1.0 mg/L, color is unchanged.
beneficial effect:
In marine alga of the present invention, lead content detects Reagent evaluation and can detect whether lead content exceeds standard more rapidly for prior art, and the accuracy rate measuring content is high, has higher detection efficiency.
Embodiment
Below the specific embodiment of the present invention is described in detail.
embodiment 1
In marine alga, lead content detects reagent, by weight, comprises following component: Phen 3 parts, sodium hydroxide solution 12 parts, ammonium citrate 3 parts, dihydroxyacetone 5 parts, sherwood oil 3 parts, dimethylglyoxime 1 part, carbon disulphide 1 part, mercuric chloride 1 part, isoprene 5 parts, ethanol 10 parts and 40 parts, water.
embodiment 2
In marine alga, lead content detects reagent, by weight, comprises following component: Phen 4 parts, sodium hydroxide solution 14 parts, ammonium citrate 4 parts, dihydroxyacetone 6 parts, sherwood oil 4 parts, dimethylglyoxime 2 parts, carbon disulphide 1 part, mercuric chloride 1 part, isoprene 7 parts, ethanol 12 parts and 50 parts, water.
embodiment 3
In marine alga, lead content detects reagent, by weight, comprises following component: Phen 4 parts, sodium hydroxide solution 15 parts, ammonium citrate 4 parts, dihydroxyacetone 6 parts, sherwood oil 5 parts, dimethylglyoxime 2 parts, carbon disulphide 1 part, mercuric chloride 1 part, isoprene 7 parts, ethanol 13 parts and 52 parts, water.
embodiment 4
In marine alga, lead content detects reagent, by weight, comprises following component: Phen 5 parts, sodium hydroxide solution 15 parts, ammonium citrate 5 parts, dihydroxyacetone 7 parts, sherwood oil 5 parts, dimethylglyoxime 3 parts, carbon disulphide 2 parts, mercuric chloride 1 part, isoprene 8 parts, ethanol 14 parts and 54 parts, water.
embodiment 5
In marine alga, lead content detects reagent, by weight, comprises following component: Phen 5 parts, sodium hydroxide solution 16 parts, ammonium citrate 5 parts, dihydroxyacetone 8 parts, sherwood oil 5 parts, dimethylglyoxime 3 parts, carbon disulphide 3 parts, mercuric chloride 2 parts, isoprene 10 parts, ethanol 15 parts and 60 parts, water.
comparative example 1
Adopt GB5009.12-2010 to measure lead content in marine alga whether to exceed standard, record detection time.
performance test
Standard: in national regulation marine alga, lead content is lower than 0.5mg/Kg.
Method of testing:
(1) seaweed sample is air-dry, grinding, crosses 100 ~ 500 mesh sieves, adds HNO
3in solution, then put into ultrasonic washing instrument, heating, ultrasonic process, take out and leave standstill cooling, centrifugal, obtain seaweed sample solution, for subsequent use;
(2) get the detection reagent of two parts of same volumes, detect in reagent to first part and add the obtained seaweed sample solution of step (1), form the first mixed liquor; Detect in reagent to second part and add the deionized water equal with seaweed sample liquor capacity, form the second mixed liquor;
(3) with the second mixed liquor for reference, observe the first mixed liquor color change, when lead content is higher than 1.0 mg/L, solution becomes blueness from aubergine, and when content of beary metal is lower than 1.0 mg/L, color is unchanged.
Adopt the lead content of embodiment 1 ~ 5 to detect reagent and measure same seaweed sample, and record time and the lead content that color starts change, the results are shown in following table:
。
As seen from the above table, whether lead content detection reagent detection lead content of the present invention exceeds standard and only needs 2.0 ~ 3.3min, and need 4.6min according to the method detection of GB5009.12-2010, and the testing result indifference of testing result and GB5009.12-2010, the detection reagent that wherein embodiment 3 is obtained is the sensitiveest, is preferred example.
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (5)
1. in marine alga, lead content detects reagent, it is characterized in that, by weight, comprise following component: Phen 3 ~ 5 parts, sodium hydroxide solution 12 ~ 16 parts, ammonium citrate 3 ~ 5 parts, dihydroxyacetone 5 ~ 8 parts, sherwood oil 3 ~ 5 parts, dimethylglyoxime 1 ~ 3 part, carbon disulphide 1 ~ 3 part, mercuric chloride 1 ~ 2 part, isoprene 5 ~ 10 parts, ethanol 10 ~ 15 parts and 40 ~ 60 parts, water.
2. in marine alga according to claim 1, lead content detects reagent, it is characterized in that, by weight, comprise following component: Phen 4 ~ 5 parts, sodium hydroxide solution 14 ~ 15 parts, ammonium citrate 4 ~ 5 parts, dihydroxyacetone 6 ~ 7 parts, sherwood oil 4 ~ 5 parts, dimethylglyoxime 2 ~ 3 parts, carbon disulphide 1 ~ 2 part, mercuric chloride 1 part, isoprene 7 ~ 8 parts, ethanol 12 ~ 14 parts and 50 ~ 54 parts, water.
3. in marine alga according to claim 1, lead content detects reagent, it is characterized in that, by weight, comprise following component: Phen 4 parts, sodium hydroxide solution 15 parts, ammonium citrate 4 parts, dihydroxyacetone 6 parts, sherwood oil 5 parts, dimethylglyoxime 2 parts, carbon disulphide 1 part, mercuric chloride 1 part, isoprene 7 parts, ethanol 13 parts and 52 parts, water.
4. in the marine alga according to any one of claim 1 ~ 3, lead content detects reagent, and it is characterized in that, described concentration of sodium hydroxide solution is 15 ~ 35wt%.
5. detect the detection method of reagent based on lead content in marine alga according to claim 1, it is characterized in that, comprise the following steps:
(1) seaweed sample is air-dry, grinding, crosses 100 ~ 500 mesh sieves, adds HNO
3in solution, then put into ultrasonic washing instrument, heating, ultrasonic process, take out and leave standstill cooling, centrifugal, obtain seaweed sample solution, for subsequent use;
(2) get the detection reagent of two parts of same volumes, detect in reagent to first part and add the obtained seaweed sample solution of step (1), form the first mixed liquor; Detect in reagent to second part and add the deionized water equal with seaweed sample liquor capacity, form the second mixed liquor;
(3) with the second mixed liquor for reference, observe the first mixed liquor color change, when lead content is higher than 1.0 mg/L, solution becomes blueness from aubergine, and when content of beary metal is lower than 1.0 mg/L, color is unchanged.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108732165A (en) * | 2018-08-15 | 2018-11-02 | 山东五洲检测有限公司 | A kind of reagent and preparation method thereof of detection Lead Content in Foodstuff |
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| CN108732165A (en) * | 2018-08-15 | 2018-11-02 | 山东五洲检测有限公司 | A kind of reagent and preparation method thereof of detection Lead Content in Foodstuff |
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Application publication date: 20150923 |