CN104931314B - A kind of processing method of fecal specimens and its application - Google Patents
A kind of processing method of fecal specimens and its application Download PDFInfo
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- CN104931314B CN104931314B CN201510297661.XA CN201510297661A CN104931314B CN 104931314 B CN104931314 B CN 104931314B CN 201510297661 A CN201510297661 A CN 201510297661A CN 104931314 B CN104931314 B CN 104931314B
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Abstract
The present invention provides a kind of processing method of fecal specimens and applications, and described method includes following steps：Fecal specimens to be measured are placed in resealable container；Drier is added into container；Container is sealed, drier absorbs the moisture in fecal specimens and in air.The processing method of the present invention effectively keeps the stability that ingredient is detected in fecal specimens, makes it possible that patient's self-service sample extraction at home, the dejecta treatment method will not hinder detected downstream（Immunology detection）, the mixture of excrement and drier can be directly entered testing process, greatly facilitate the follow-up processing flow flexibility of fecal specimens, contribute to the detection and treatment of disease of digestive system.
Description
Technical field
The invention belongs to biochemical field, it is related to processing method and its application of a kind of fecal specimens.
Background technology
Be presently used for disease detection fecal specimens it is unstable be difficult to preserve, therefore the excrement sample of hospital or medical center
Product are chemically examined, it is necessary to inspection immediately.This causes certain inconvenience to patient, also increases work load to medical staff, meanwhile,
Also it increases running to testing laboratory and the difficulty of result is provided in time.To cause patient to coordinate under the ratio for providing sample
Drop makes the validity of follow-up relevant medical procedure also be affected.
Although the existing relevant protection liquid of research and development, technical sophistication is expensive, and transports inconvenience, may cause liquid
Leakage.Current main detection method is still detected in hospital's field sampling, and Chinese population radix is excessive, is only detected with hospital
It is the demand that much cannot be satisfied our people and increasingly improve health examination.Therefore, a kind of low price is developed, safety, effectively
Fecal specimens antihunt means are a problems for being medically badly in need of solving at present.
Invention content
The present invention is directed to disadvantage mentioned above, provides a kind of fecal specimens processing method, reaches cheap safety, while can be effective
The stability for being detected ingredient in fecal specimens, nontoxic or corrosion composition is kept not to interfere detected downstream, easy-to-operate
Purpose.
To achieve the above object, the present invention takes following technical proposals to realize：
A kind of processing method of fecal specimens, includes the following steps：
Fecal specimens to be measured are placed in resealable container；
Drier is added into container；
Container is sealed, drier absorbs the moisture in fecal specimens and in air.
Further, the water content after drying in fecal specimens is less than 10%.
Further, the drier is silica or activated alumina.
Preferably, drier is activated alumina.
Further, the grain size of activated alumina is less than 10mm.
Preferably, the grain size of activated alumina is 1-2mm.
Further, the dosage of activated alumina is 1.5-3 times of fecal specimens weight.
Further, the quality of the fecal specimens of merging with can closed container capacity ratio be 0.05-0.15g/mL.
The present invention also provides application of the processing method of above-mentioned fecal specimens in detecting disease of digestive system.The present invention's
Fecal specimens processing method can be used for detecting protein, nucleic acid equal samples in fecal specimens, all kinds of so as to be used to detect
Disease of digestive system, including hemorrhage of gastrointestinal tract, all kinds of malignant tumours are such as：Gastric cancer, colorectal cancer etc..
Further, the present invention provides application of the processing method of above-mentioned fecal specimens in detecting colorectal cancer.
In short, beneficial effects of the present invention are：
The present invention makes fecal specimens be dehydrated by the method for adding drier, such as silica, activated alumina etc..Added
The drier added is in together with fecal specimens in a closed space, and drier can directly absorb the moisture in sample, also may be used
The moisture in air is absorbed, finally makes the state that sample is in drying and dehydrating stable to achieve the effect that.
The mass propagation of bacterium will produce a large amount of metabolic waste, and decomposite various enzymes, and the above exactly fecal specimens are not
The main reason for stablizing, under the dry condition, the growth and breeding of bacterium is in holddown；Various enzymes, such as protease, DNA
Enzyme can directly degrade the detected material in sample, but all enzymes only have it is just active in a liquid state, when drying condition, enzyme
Activity almost lose；Tested composition in excrement is with protein, and based on DNA, RNA, these compositions are in liquid condition
It is unstable but with good stability in the solid state, and dry method can make the above substance be maintained at solid-like
State.
The fecal specimens processing method of the present invention is easy to operate, and can effectively keep being detected the steady of ingredient in fecal specimens
It is qualitative, make it possible that patient's self-service sample extraction at home, the excrement stabilizing solution will not hinder detected downstream (immunology inspection
Survey), the mixture of excrement and drier can be directly entered testing process, greatly facilitate the subsequent processing stream of fecal specimens
Journey flexibility contributes to the detection and treatment of disease of digestive system.
Drier used in the present invention is to commonly use substance in usually living, and is different from other biological product, does not have to the mankind
Have it is any potentially hazardous, have environmentally protective, safe to use advantage.
High-tech product utilization rate in the not high crowd of the elderly and educational level is not high, mainly due to its specification depth
Difficult to understand hard to understand, operation is excessively complicated.Though the Related product of the present invention is high-tech product, explanation and use are very simple, are easy to
By the elderly and educational level, relatively not high crowd receives.The malignant tumours such as colorectal cancer elderly population incidence very
Height, certain hotspots, if Haining reaches 6 ﹪ or more, the present invention can be very good to improve the pole that the elderly detects intestinal cancer
Product property.
In short, the cheap safety of processing method of the present invention, while can effectively keep being detected ingredient in fecal specimens
Stability, nontoxic or corrosion composition do not interfere detected downstream, easy-to-operate；It can be used for the detection of all kinds of digestive diseases, it is special
It is not the detection of the malignant tumours such as gastric cancer, colorectal cancer.
Further, drier composition used in the present invention is cheap, by taking activated alumina as an example, only 8 yuan of per kilogram, with
Every part of amount of samples 12g is calculated, and cost is 0.1 yuan of per person.Many economy fall behind relatively at present, medical condition wretched insufficiency
Most of people in area be both without access to the service of hospital, can not also undertake expensive biological technology products.The present invention is great
Production cost is reduced, it is cheap effective, the ordinary consumer in these places can be made to be able to enjoy disease detection screening at a low price
Service.
Further, the activated alumina grain size that the present invention uses is smaller, and dispersibility is better, and rate of drying is also faster, has
Conducive to the decomposition of object to be measured in reduction fecal specimens.
Specific implementation mode
With reference to specific embodiment, the present invention will be described in detail.
Embodiment 1：
Two groups of experiments are set, pipe 1 and pipe 2 be can closed container, be separately added into 5g fecal specimens in middle pipe 1 and pipe 2,
(fecal specimens are detected as feminine gender through immunogold assay Test paper (FOB)), is then added into two pipes respectively
100ug/ml human hemoglobins.
12g activated alumina desiccants (wherein a diameter of 1mm -2mm of activated alumina) are added in pipe 1, are then sealed
Stopped pipe body；Pipe 2 as a contrast, is not added with drier closed tube.The above sample was stored in 25 degrees Celsius, in the 0th, 1,2,3,6,10 day
FOB detections are done, record the minimum hemoglobin concentration that can be detected respectively.
Detection method：It takes sample in pipe to carry out gradient dilution, records maximum dilution multiple, original blood is removed with this number
Hemoglobin concentration 100ug/ml, obtains after preservation, can detect hemoglobin concentration minimum in excrement.The 0th in experiment
It testing result is 0.2ug/ml, this is the Monitoring lower-cut of FOB test paper.For excrement after preservation, excrement sample can be detected by comparing
The variation of minimum hemoglobin in product, it is to be understood that whether hemoglobin is stablized in excrement.
Testing result is as shown in table 1.
1 25 degrees Celsius of table preserves the minimum hemoglobin concentration that the FOB detection methods of lower sample can detect
| Time | 0 day | 1 day | 2 days | 3 days | 6 days | 10 days |
| Pipe 1 | 0.2ug/ml | 0.4ug/ml | 0.4ug/ml | 0.4ug/ml | 0.4ug/ml | 1ug/ml |
| Pipe 2 | 0.2ug/ml | 0.5ug/ml | 2ug/ml | 20ug/ml | It can not detect |
As shown in table 1, processing method using the present invention adds the pipe 1 of activated alumina desiccant, can be in 6 days
The minimum hemoglobin concentration detected is 0.4ug/ml, until the 10th day is 1ug/ml, therefore at least 10 days, in pipe 1
Hemoglobin in fecal specimens is stabilized, and can be detected.And pipe 2 as a contrast, it degrades, arrives on day 1
2nd day, minimum hemoglobin concentration just became initial 10 times, can not detect completely within the 6th day.
Embodiment 2
Two groups of experiments are set, pipe 1 and pipe 2 be can closed container, be separately added into 5g fecal specimens in middle pipe 1 and pipe 2,
100ug/ml human hemoglobins are then added in (fecal specimens are detected as feminine gender through FOB) into two pipes respectively.
12g activated alumina desiccants (wherein a diameter of 1mm -2mm of activated alumina) are added in pipe 1, are then sealed
Stopped pipe body；Pipe 2 as a contrast, is not added with drier closed tube.The above sample was stored in 37 degrees Celsius, in the 0th, 1,2,3,6,10 day
FOB detections are done, record the minimum hemoglobin concentration that can be detected respectively.
Detection method：It takes sample in pipe to carry out gradient dilution, records maximum dilution multiple, original blood is removed with this number
Hemoglobin concentration 100ug/ml, obtains after preservation, can detect hemoglobin concentration minimum in excrement.The 0th in experiment
It testing result is 0.2ug/ml, this is the Monitoring lower-cut of FOB test paper.For excrement after preservation, excrement sample can be detected by comparing
The variation of minimum hemoglobin in product, it is to be understood that whether hemoglobin is stablized in excrement.
Testing result is as shown in table 2.
2 37 degrees Celsius of table preserves the minimum hemoglobin concentration that the FOB detection methods of lower sample can detect
| Time | 0 day | 1 day | 2 days | 3 days | 6 days | 10 days |
| Pipe 1 | 0.2ug/ml | 0.5ug/ml | 0.5ug/ml | 0.5ug/ml | 1ug/ml | 2ug/ml |
| Pipe 2 | 0.2ug/ml | 1ug/ml | 10ug/ml | It can not detect |
As shown in table 2, under hot conditions, the decomposition rate of sample is faster.And processing method using the present invention, addition are lived
The pipe 1 of property alumina desiccant, in 3 days, the minimum hemoglobin concentration that can be detected is 0.5ug/ml；By the 6th day,
For 1ug/ml.Therefore at least 6 days, the hemoglobin in fecal specimens in pipe 1 is stabilized, and can be detected.And conduct
The pipe 2 of control is degraded on day 1, and by the 2nd day, minimum hemoglobin concentration just became initial 50 times, and the 3rd day i.e.
It can not detect completely.
And in practice, the blood content of the fecal occult blood positive just has clinical medicine meaning than normal high decades of times, so detection
As long as the reduction of sensitivity is no more than 10 times, there is practical value.According to China express delivery speed, national most area
Sample in that can be reached in 6 days, protect by the processing method of the data in above-described embodiment 1 and embodiment 2, fecal specimens of the present invention
Storage sample product are effective.In addition, when seasoning preserves sample, the unstability of sample occurs mainly in 24 hours after sampling
Interior, sample at this time is not also fully dry, and part checking matter is degraded at this time.And the stability in sample later stage is relatively high, this
It is very favorable for reducing the error that different haulage times generate.
Application example：
Cooperation Hangzhou hospital carries out mirror to the intestinal cancer people at highest risk of Zhejiang rural area 50 years old or more and looks into, using this hair
Bright method preserves the fecal specimens of examinee, and carries out FOB detections.The result of FOB analyzes quilt as one of evaluation parameter
Inspection person suffers from the risk of intestinal cancer.The specific method is as follows：
50ml, which can be closed in probe tube, is added about 12g active oxygen assumed name's aluminium drier, and attached there are one sample spoon, examinee
It takes a scoop sample product (about 5g) to probe tube, then returns, carry out FOB detections.In practical flow, carried out from sampling to returning
FOB detection times are about 4-7 days, and during which sample is in room temperature.After tester receives sample, 30ml is added water to, fills a part mixing
Sample dissolution carries out colloidal gold method FOB detections, and reads signal strength, and contrast standard product calculate blood red in tested excrement
Protein content.
Key parameter processing method when specific calculating is as follows：(1) it after adding water to 30ml, since raw manure is 5g, presses
Sample is diluted 6 times of calculating；(2) sample needs can just be detected for 4-7 days after sampling, according to existing experimental studies results, sample
Product (after sampling 24 hours) when early period is unseasoned abundant have Partial digestion, and sensitivity can reduce by 2 times, so calculating correction
When, baseline results are multiplied by 2.
It is the partial data of testing result below
The FOB data of 3 intestinal cancer people at highest risk's fecal specimens of table detection
| Number | Raw manure hemoglobin concentration ug/ml |
| 9600 | 0.288 |
| 9609 | 0.294 |
| 2934 | 0.51 |
| 9746 | 0.576 |
| 9730 | 0.78 |
| 9586 | 1.164 |
| 9578 | 2.76 |
| 9603 | 2.85 |
| 2929 | 3.408 |
| 9745 | 4.098 |
| 2704 | 4.368 |
| 9561 | 4.506 |
| 9556 | 4.866 |
| 9623 | 7.35 |
| 9744 | 7.356 |
| 9748 | 7.812 |
| 9702 | 7.878 |
| 2941 | 7.908 |
| 9528 | 8.166 |
| 9708 | 8.652 |
| 9694 | 8.748 |
| 9553 | 8.916 |
| 9640 | 8.982 |
| 9636 | 12.33 |
| 9705 | 16.146 |
| 2844 | 16.32 |
| 9554 | It is negative |
| 9594 | It is negative |
| 9632 | It is negative |
| 9719 | It is negative |
| 9610 | It is negative |
| 9624 | It is negative |
| 9631 | It is negative |
| 9606 | It is negative |
| 9680 | It is negative |
| 9574 | It is negative |
| 9634 | It is negative |
| 9658 | It is negative |
| 9692 | It is negative |
| 2620 | It is negative |
| 9633 | It is negative |
| 2943 | It is negative |
| 2721 | It is negative |
| 9601 | It is negative |
| 9657 | It is negative |
| 9643 | It is negative |
| 9555 | It is negative |
| 9630 | It is negative |
| 9654 | It is negative |
| 9566 | It is negative |
| 9604 | It is negative |
| 2932 | It is negative |
| 9665 | It is negative |
| 9686 | It is negative |
| 9596 | It is negative |
55 parts of samples are had detected in this example altogether, wherein 26 parts of FOB test positive, 29 parts are feminine gender, and positive rate is close
Half is higher than hospital's routine FOB detection sensitivities.Since this experiment is directed to intestinal cancer people at highest risk, FOB Positive rate height is just
Often occur as, while high positive rate illustrate the present invention sample treatment respectively be effective.
Although the invention has been described by way of example and in terms of the preferred embodiments, but it is not for limiting the present invention, any this field
Technical staff without departing from the spirit and scope of the present invention, may be by the methods and technical content of the disclosure above to this hair
Bright technical solution makes possible variation and modification, therefore, every content without departing from technical solution of the present invention, and according to the present invention
Technical spirit any simple modifications, equivalents, and modifications that above example is made, belong to the technology of the present invention side
The protection domain of case.
Claims (8)
1. a kind of processing method of fecal specimens, for detecting the protein in excrement, which is characterized in that the method includes such as
Lower step：
Fecal specimens to be measured are placed in resealable container；
The mixture that drier forms excrement and drier is added into container；
Container is sealed, drier absorbs the moisture in fecal specimens and in air；
Water content after drying in fecal specimens is less than 10%.
2. processing method according to claim 1, which is characterized in that the drier is silica or active oxidation
Aluminium.
3. processing method according to claim 2, which is characterized in that drier is activated alumina.
4. processing method according to claim 2, which is characterized in that the grain size of activated alumina is less than 10mm.
5. processing method according to claim 3, which is characterized in that the dosage of activated alumina is fecal specimens weight
1.5-3 again.
6. processing method according to claim 1, which is characterized in that the quality of the fecal specimens of merging with can closed container
Capacity ratio be 0.05-0.15g/mL.
7. application of the processing method of fecal specimens as described in claim 1 in detecting disease of digestive system.
8. application of the processing method of fecal specimens as described in claim 1 in detecting colorectal cancer.
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