CN104922098A - Application of natural fat-soluble component - Google Patents
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Abstract
The invention belongs to the field of biomedicine and health and specifically relates to an application of a natural fat-soluble component. The natural fat-soluble component is used as a substance for increasing quantity and vitality of male germ cells (sperms). The natural fat-soluble component is used as food, a beverage, a pharmaceutical health care product or a biological agent for increasing quantity and vitality of male germ cells (sperms). By the utilization of unicellular algae obtained by cell engineering large-scale culture, a carotenoid-rich substance and its oxidation derivative natural fat-soluble component are extracted. On this basis, through laboratory animal feeding verified experiments, a pharmaceutical health care product or a biological agent is developed and applied in increasing quantity and vitality of male germ cells (sperms) and enhancing fecundity.
Description
Technical field
The present invention is subordinate to biomedicine and health field, specifically a kind of application of natural lipoclastic.
Background technology
Sexual cell (sperm) quantity of buck, concentration and vigor thereof are the key factors determining its reproductive function.Oligoasthenospermia (asthenospermia) disease is usually the one of the main reasons causing male sterility or fertility to decline.With artificial example, there is a large amount of bending contorted seminiferous tubules in testis, therebetween containing Interstitial cell.Male sex-cell is grown by spermatogonium and is formed, produce sexual cell in contorted seminiferous tubules after, be released in seminiferous tubule tube chamber, now spermatid itself does not still have motor capacity, but is transported in epididymis by the contraction of tubule periphery myoid cell and the movement of liquid lumen.In epididymis, sexual cell is ripe further, and obtains motor capacity.Can store sexual cell in a small amount in epididymis, a large amount of sexual celies is then stored in deferent duct and ampulla thereof.The secretions of sexual cell and epididymis, seminal vesicle, prostate and cowper gland is mixed to form seminal fluid, containing 2,000 ten thousand-4 hundred million sexual celies in every milliliter of seminal fluid, in normal male seminal fluid, sperm count is at every milliliter more than 6,000 ten thousand, but when sperm quantity is less than 2,000 ten thousand then in oligospermatism; And azoospermia refers to that nitric oxide difference is even without mobility.Few essence and azoospermia are all difficult to make ovum fertilization usually, cause male sterility or fertility to decline.
Male sex-cell buds into ripe sexual cell from spermatogonium, needs through following different stage and process: spermatogonium → primary spermatocyte → secondary spermatocyte → sexual cell cell → sexual cell.The sex hormone level of the formation of number of germ cells and vigor and biology itself has relation directly or indirectly, and the power of number of germ cells and vigor is also the Microscopic form of sex hormone level height.Namely number of germ cells and vigor are actually the imbody of the important function that sex hormone level is played the part of in different levels such as metabolic activity in cells, individual growth growths, simultaneously, number of germ cells is also the important symbol feature of number of germ cells and vigor, and in the choosing spouse of female, Sexual behavior mode process, play indispensable key player further, biosystem is grown, procreation reproduction and biological evolution process play pivotal role.
Although the incredible amount of sexual cell sperm, be also very active, simultaneously the vitality of sexual cell sperm is also very fragile, and what be very easy to be subject to many factors affects damage and inactivation.Such as: the impact being vulnerable to variations in temperature in sexual cell production process, if testis environment temperature is too high or be subject to the impact of chemical toxicant, sexual cell produces will there is obstacle; Meanwhile, varicocele, epididymis are blocked, inflammation, congenital deferent duct or other genitals's aplasia or dysplasia occur prostate; In addition, in recent decades, coerce by many environmental factorss, eating patterns etc. constantly changes, and the comprehensive complex effects of smoking and drinking and air environmental pollution or life stress, the trend that a lot of biology has occurred that number of germ cells and vigor weaken more or less, degenerated, even feminizes, continues to occur declining with surprising speed in the number of germ cells of youth of marriageable age's males and vigor.In addition, along with social development, young people old enough to do sth are busy with cause or philosophy of life change, cause constantly postpone marriageable age, more exacerbate above-mentioned phenomenon and occur.
The Rapid Variable Design trend of above-mentioned sexual cell sperm quantity and vigor, cause difficulty to educate to present with the phenomenon such as sterile and rise appreciably, all can produce bion growth or phylogeny and affect to some extent, as the little not obvious change faint to external appearance characteristic, produce fatal irreversibility effect etc. to multiplying existence for a long time to reproduction and species greatly.How to increase the number of germ cells of male creature, density and vigor, the key subjects of basic life science and the needs concern of medical health field will be become gradually.Still difficulty has very effective biological preparation, medicine or health promoting product at present.
Under certain environmental conditions, some unicellular alga can accumulation carotenoid and oxidized derivatives thereof in a large number, such as, salt alga accumulates the carotenoid based on beta-carotene under high salt, high temperature and high light intensity, and Haematocoocus Pluvialls can accumulate the beta-carotene oxidized derivatives based on astaxanthin in a large number.The carotenoid of above-mentioned microalgae and oxidized derivatives thereof possess the plurality of advantages such as natural sex, trophism, edible safety and avirulence, and have and unique catch free radical and anti-oxidation function.Effectively utilize the ability of above-mentioned unicellular alga accumulation natural carotenoid and derivant thereof, be expected to produce acquisition natural grease soluble mixture matter by biological engineering Technology.These natural fat-soluble compounding substances comprise the lipid materials such as carotenoid and oxidized derivatives thereof such as being rich in beta-carotene, astaxanthin, zeaxanthin, phylloxanthin.What utilize above-mentioned liposoluble substance uniqueness catches free radical and anti-oxidation function, be expected to develop with the main biological preparation such as natural carotenoid and derivant thereof, realize the object of the number of germ cells of improvement or increase male creature, density and vigor to a certain extent.
Summary of the invention
The object of the invention is the application providing a kind of natural lipoclastic.
the technical solution used in the present invention is for achieving the above object:
An application for natural lipoclastic, natural lipoclastic is as the material increasing male creature sexual cell (sperm) quantity and vigor.
Described natural lipoclastic is as increasing the food of male creature sexual cell (sperm) quantity and vigor, beverage, Medicines and Health Product or biological preparation.
Liposoluble substance is natural carotenoid and oxidized derivatives thereof, or through with unicellular alga be the accumulation of raw material stress-inducing acquire be rich in carotenoid and oxidized derivatives thereof.
Described natural carotenoid and oxidized derivatives thereof, wherein main component comprises: carotenoid and the oxidized derivatives thereof such as beta-carotene, astaxanthin, zeaxanthin, phylloxanthin, and other the fat-soluble compounding substances containing some fatty acid.
Wherein, be that raw material acquires carotenoid and oxidized derivatives thereof through stress-inducing accumulation with unicellular alga, be specially:
First, select unicellular alga, with two step series connection culture techniques, cultivate in first step scale cell engineering and obtain Biomass; Then, change condition of culture, utilize stress-inducing its a large amount of accumulation carotenoid and oxidized derivatives thereof at second step;
Then, cell engineering is cultivated microalgae cell results, and namely processing obtain being rich in carotenoid and oxidized derivatives algae powder thereof, or utilize physico-chemical process to extract the liposoluble substance that acquisition is main active with carotenoid and oxidized derivatives thereof.
Described unicellular alga is salt alga (Dunaliella salina or bardawil) or Haematocoocus Pluvialls (Haematococcus pluvialis or lacustris).
Calculate according to the male of 60kg body weight, described natural carotenoid and the general >5.0mg of oxidized derivatives Day feeding amount thereof, general use amount controls in 15.0mg-100.0mg/ people/daily range.
For realizing the present invention, can the microalgae kind of a large amount of accumulation carotenoid and oxidized derivatives thereof under given conditions a lot, mainly comprise: Dunaliella salina (is called for short salt alga, Dunaliella salina or bardawil) and Haematocoocus Pluvialls (being called for short Haematococcus Pluvialis, Haematococcus pluvialis or lacustris) etc.Simultaneously, the primary bioactive components carotenoid of indication of the present invention and the kind of oxidized derivatives thereof, also abnormal rich and varied in microalgae, mainly comprise carotenoid and the oxidized derivatives thereof such as alpha-carotene, beta-carotene, astaxanthin, zeaxanthin, phylloxanthin, lycopene.In algae powder and liposoluble substance thereof, simultaneously also containing other lipid soluble ingredients such as some fatty acids.In addition, effective active matter for increasing male creature number of germ cells and viable product is carotenoid and oxidized derivatives thereof, can be compounding substances, also can be the one-component of further extraction purification, and according to the content of active substance and the concrete animal species of practical application and body weight etc., that converts its concrete every day according to relevant criterion makes (food) consumption or food ration.
Advantage of the present invention is:
1. the present invention by carotenoid and oxidized derivatives thereof for increasing male creature number of germ cells and vigor, its product is not synthetic, belong to the liposoluble substance (or its algae powder) extracted in some unicellular microalgae, therefore, product is from the advantage with natural sex.
2. the present invention is by carotenoid and oxidized derivatives thereof the lipoclastic for increasing male creature number of germ cells and vigor, wherein the effective ingredient of indication is mainly carotenoid and oxidized derivatives thereof, belong to the reducing substances (although the content of general plant is very low) produced in normal plants metabolic process, what have uniqueness catches free radical and anti-oxidation function, play important supporting role to maintaining organism metabolism balance, there is very high Nutrition.
3. the present invention is by carotenoid and oxidized derivatives thereof the lipoclastic for increasing male creature number of germ cells and vigor, wherein the effective ingredient of indication is mainly carotenoid and oxidized derivatives thereof, belong to one of organic material component of animals and plants the most basic (although content is general lower), decompose by homergy in human body, not long term accumulation; Eat in history for a long time the mankind thousands of years mankind, do not find its toxic and side effects; Product, through the toxicological experiment of zoology model, does not find any toxic and side effects, has long-term edible safety.
4. the present invention is by carotenoid and oxidized derivatives thereof the lipoclastic for increasing male creature number of germ cells and vigor, wherein the effective ingredient of indication is mainly carotenoid and oxidized derivatives thereof, method by artificial culture and biological engineering technique is produced, is extracted and processing, therefore this product quality has human controllable's property, can ensure the stable performance of its effect.
5. the present invention is by carotenoid and oxidized derivatives thereof the lipoclastic for increasing male creature number of germ cells and vigor, on animal experiment study working foundation, newly excavate carotenoid and oxidized derivatives thereof to have and increase male creature number of germ cells and vigor, new type of health product and biomedical product can be developed, carry out application in association area and there is science and reliability.
6. the present invention is by fat-soluble for increasing male creature number of germ cells and vigor of carotenoid and oxidized derivatives thereof, and it possesses special biological effectiveness, therefore should have that very high market development is worth, economic benefit and a social value.
Accompanying drawing explanation
Fig. 1 is the high pressure liquid chromatography figure of Haematococcus Pluvialis natural astaxanthin and synthetic astaxanthin product
Detailed description of the invention
A lot of based on the microalgae kind of accumulation carotenoid and oxidized derivatives thereof, wherein most typical kind comprises under given conditions, the Dunaliella salina that can accumulate in a large number based on beta-carotene (is called for short salt alga, Dunaliella salina or bardawil), and based on the Haematocoocus Pluvialls accumulating beta-carotene oxidized derivatives astaxanthin in a large number (being called for short Haematococcus Pluvialis, Haematococcus pluvialis or lacustris) etc.For this reason, only for Haematocoocus Pluvialls, produced by cell engineering Technology and obtain natural grease soluble mixture matter.These natural fat-soluble compounding substances comprise the lipid materials such as carotenoid and oxidized derivatives thereof such as being rich in beta-carotene, astaxanthin, zeaxanthin, phylloxanthin.And then illustrate raw material sources of the present invention, processing technique and product improve male creature some male characteristic effect, make (food) with the embodiment such as dosage or food ration.
Embodiment 1
A. being rich in astaxanthin is that main Haematococcus Pluvialis is cultivated
Select Haematocoocus Pluvialls, make culture fluid with the culture medium prescriptions such as BG1 (see table 1), under 22-25 DEG C of condition, with Indoor Natural stray light or auxiliary with 50 μ Em
2-s
-1the light illumination (the illumination rhythm and pace of moving things of 14:10) of intensity of illumination, cultivates Haematococcus Pluvialis algae kind.
Treat Haematococcus Pluvialis algal species cultivation 10-12 days, cell is in the exponential phase of Fast-propagation.The algae kind being cultured to exponential phase be inoculated into in new production culture fluid, then continue to cultivate in the training workshop of 20-25 DEG C, cultivate and adopt natural daylight light source, intensity of illumination is by abat vent regulable control, and intensity is at 1000-2000 μ Em
2-s
-1in scope, continue amplification culture 2 weeks.
By the Haematococcus Pluvialis algae strain of amplification culture, then be transferred in open raceway pond bioreactor by closed pipe system.Culture medium (according to table 1 formula, after removal compound fertilizer, namely obtaining the culture medium that the nitrogen and phosphorus wanes) inducing cell utilizing nitrogen phosphorus to wane transforms, and swarm cell is converted into non motile cell in a large number, one-step inducing astaxanthin and an oil accumulation of going forward side by side.Inductive condition, light source adopts natural daylight, and its intensity of illumination maintains 1000-3000 μ Em by day substantially
2-s
-1between change, about 14 days artificial culture time, period, algae liquid water temperature was with weather natural trend between 15-33 DEG C, and pH value slowly rises to 10.0 from initial 7.5, even higher.Between culture period, algae liquid relies on waterwheel stir and flow and avoid cell to sink, and ensures that Haematococcus Pluvialis non motile cell is fully exposed to sunlight, accumulates astaxanthin in a large number simultaneously.
B. the Product processing of the carotenoid and isomer etc. thereof of astaxanthin is rich in
Results rely on natural subsidence that frond is deposited, and bleed off supernatant, concentrated algae liquid; So repeatedly, denseer algae liquid is progressively obtained; Finally adopt centrifugal acquisition algae mud, and repeatedly clean algae mud with only clean Natural Water.Fresh algae is spray dried to dry algae powder, then utilizes ultrahigh speed to touch and carry out cell wall breaking processed, to increase its bioavailability.Above-mentioned algae powder can make direct material, and being directly used in development increases the product of male creature number of germ cells and vigor and apply; Also can utilize physics and chemistry technology further, extract and obtain the lipoclastic such as its carotenoid and oxidation product astaxanthin thereof, being used further to development increases the product of male creature number of germ cells and vigor and applies.
Fat-soluble product by analysis, its main component is as table 2, and wherein principle active component is carotenoid and oxidation product thereof, and content affects by cultivation and extracting parameter and there is certain change, generally accounting for the 2.0%-8.0% of lipoclastic, wherein minimum and most high-load is respectively 0.5% and 15.0%.In any case but in carotenoid and oxidation product thereof, main component, based on astaxanthin, accounts for the 75%-95% of its content.
Utilize high pressure liquid phase analysis further, obtain Haematococcus Pluvialis natural astaxanthin high pressure liquid chromatography figure and synthetic contrasts collection of illustrative plates (as Fig. 1), documents data can determine that produced Haematococcus Pluvialis natural astaxanthin is based on left-handed spiral configuration, that is: Haematococcus Pluvialis astaxanthin is with left-handed type (3S, 3 ' S) be main, and the conformation be different from Synthetic artifact astaxanthin, left-handed (the 3S of optical siomerism of the latter, 3 ' S) type, racemization (3R, 3 ' S) ratio of type and dextrorotation (3R, 3 ' R) type is 1:2:1.
Several common culture medium composition for Haematococcus Pluvialis cultivation of table 1. and formula thereof
Table 2. is rich in bioactive ingredients---the Haematococcus Pluvialis liposoluble substance composition of carotenoid and oxidized derivatives thereof and content (%)
Embodiment 2
Buck is fed and some number of germ cells and vigour-testing method
Select the male and healthy Wistar rat (body weight 150-160g) in 30 4-5 all ages, first Health survey 1 week, is then divided into 3 groups at random, often organizes 10, puts into 1 cage for every 5.23.0 ± 3.0 DEG C of temperature, 40-70% relative humidity, 12/12h illumination (light intensity 150-300Lux), separate marking under 10-15 time/h of taking a breath also is raised.Wherein 2 groups of fat-soluble mixture of Haematococcus Pluvialis obtained by rat kg body weight above-described embodiment that gavage gives low content and moderate content respectively, wherein effective ingredient is carotenoid based on astaxanthin-containing and derivant thereof, and the effective ingredient administered dose of median dose group is 0.9mg/kg body weight, the astaxanthin of high dose group is the effective ingredient administered dose of main carotenoid is 2.7mg/kg body weight, and matched group only carries out same gavage process with the vegetable oil of equal volume.The official number of every animal is coated with dye with chemical dye at animal bodies different parts and makes marks.Continuous gavage 10 weeks, the weather of period observation animal; And the body weight of itemized record male rat and food ration, and according to the actual weight of male rat, adjustment gavage consumption every day (being rich in the carotenoid liposoluble extract of Haematococcus Pluvialis astaxanthin).
The carotenoid lipoclastic that male rat gavage is fed based on astaxanthin is after 10 weeks, and its number of germ cells and vigor and vigor respectively significant change occur.Result shows: 1). with regard to Unit Weight epididymis sexual cell density, in matched group male rat group, every gram contains sperm count 49.5 hundred million, median dose group rat every gram is 67.3 hundred million containing sperm count, and high dose group rat every gram is 84.6 hundred million containing sperm count.Therefore, compared with matched group, the sexual cell density in median dose group and high dose group male rat epididymis adds 36.0% and 71.0% respectively, and difference is extremely remarkable.2). total sperm count of the one-sided tail of epididymis of matched group male rat is 17.5 hundred million, and total sperm count of the male rat tail of epididymis of feed median dose group and high dose group is respectively 22.6 and 28.1 hundred million.That is: compared with matched group, total germ cell number of median dose group male rat one-sided (right side) tail of epididymis adds 29.0%, in significant difference; And the male rat sperm sum of high dose group adds 60.0%, in pole significant difference.
Secondly, the sperm activity of activated for tool tail of epididymis is carried out analysis show: the total activity ratio of matched group sperm is 44.2%, and the total activity ratio of the sperm of middle and high dosage group is respectively 31.5% and 34.5%, the activity ratio significance that namely sperm of middle and high dosage group is total is lower than matched group (P<0.05 or P<0.01).Because inactive sperm still can not be attributed to dead sperm cells simply, also may there is still non-activated probability, be difficult to differentiation at present and belong to death and still do not activate, need to analyze the situation enlivening sperm for this reason.In control rats epididymis, every gram contains sperm count 49.5 hundred million, and median dose group every gram containing sperm 67.3 hundred million, high dose group every gram sperm count 84.6 hundred million.Therefore, great-hearted sperm concentration in Unit Weight epididymis, is respectively every gram 21.9 hundred million, 21.2 hundred million and 29.2 hundred million in matched group, median dose group and high dose group.That is, at median dose and matched group no significant difference, comparatively contrast in high dose group and be significantly improved.
Equally, as calculated with regard to activated total germ cell number in one-sided tail of epididymis, at matched group and median dose group indistinction, and in high dose group tail of epididymis, activated total germ cell number adds 25.3%, namely has significance to increase in high dose group than matched group.
In addition, by the further classification of sperm activity of activated for tool tail of epididymis, find the III level sperm count minimizing more obvious than matched group that active degree is lower in high dose group, and there is the high II level sperm count of active degree have and increase considerably.
In sum, confirm with Haematococcus Pluvialis astaxanthin case study on implementation, high dose microalgae carotene and oxidized derivatives thereof obviously increase the II level sperm ratio of reproduction of male animal cell sperm quantity, the great-hearted sperm concentration of tool and high active degree.Therefore, utilize the liposoluble substances such as unicellular microalgae natural carotenoid and oxidized derivatives thereof, or be rich in micro-algae powder (its administered dose effective ingredient >1.35mg/kg of mentioned component, general range is at 1.35mg/Kg-9.0mg/Kg), processing food, beverage, Medicines and Health Product and biological preparation, can be used for increasing male creature number of germ cells and vigor.
List of references;
1.Turujman SA,Wamer WG,Wei RR,Albert RH，Rapid liquid chromatographic method to distinguish wild salmon from aquacultured salmon fed syntheticastaxanthin.Journal of AOAC International.,3:622–632，1997
2.Higuera-Ciapara I,Félix-Valenzuela L,Goycoolea FM，Astaxanthin:A Review of its Chemistry and Applications,Food Science and Nutrition.46:185–196，2006
3.Grung M,D’Souza FML,Borowitzka M,Liaaen-Jensen S，Algal carotenoids51.Secondary carotenoids2.Haematococcus pluvialis aplanospores as a source of(3S,3'S)-astaxanthin esters.J.Appl.Phycol.4:165-171，1992
4.Renstrom B,Borch G,Skulberg OM,Liaaen-Jensen S，Optical purity of(3S,3’S)-Astaxanthin from Haematococcus pluvialis.Phytochemistry20:2561–2564，1981
5.Turujman SA,Wamer WG,Wei RR,Albert RH，Rapid liquid chromatographic method to distinguish wild salmon from aquacultured salmon fed synthetic astaxanthin.J AOAC Int80(3):622–632，1997
6.Bowen J,Soutar C,Serwata RD,Lagocki S,White DA,Davies SJ,Young AJ，Utilization of(3S,3
S)-astaxanthin acyl esters in pigmentation of rainbow trout(Oncorhynchus mykiss).Aquaculture Nutrition，8：59-68，2002 。
Claims (7)
1. an application for natural lipoclastic, is characterized in that: natural lipoclastic is as the material increasing male creature sexual cell (sperm) quantity and vigor.
2. by the application of natural lipoclastic according to claim 1, it is characterized in that: described natural lipoclastic is as increasing the food of male creature sexual cell (sperm) quantity and vigor, beverage, Medicines and Health Product or biological preparation.
3. by the application of the natural lipoclastic described in claim 1 or 2, it is characterized in that: liposoluble substance is natural carotenoid and oxidized derivatives thereof, or through with unicellular alga be the accumulation of raw material stress-inducing acquire be rich in carotenoid and oxidized derivatives thereof.
4. by the application of natural lipoclastic according to claim 3, it is characterized in that: described natural carotenoid and oxidized derivatives thereof, wherein main component comprises: carotenoid and the oxidized derivatives thereof such as beta-carotene, astaxanthin, zeaxanthin, phylloxanthin, and other the fat-soluble compounding substances containing some fatty acid.
5., by the application of the natural lipoclastic described in claim 1 or 2, it is characterized in that:
Be that raw material acquires carotenoid and oxidized derivatives thereof through stress-inducing accumulation with unicellular alga, be specially:
First, select unicellular alga, with two step series connection culture techniques, cultivate in first step scale cell engineering and obtain Biomass; Then, change condition of culture, utilize stress-inducing its a large amount of accumulation carotenoid and oxidized derivatives thereof at second step;
Then, cell engineering is cultivated microalgae cell results, and namely processing obtain being rich in carotenoid and oxidized derivatives algae powder thereof, or utilize physico-chemical process to extract the liposoluble substance that acquisition is main active with carotenoid and oxidized derivatives thereof.
6., by the application of natural lipoclastic according to claim 5, it is characterized in that: described unicellular alga is salt alga (Dunaliella salina or bardawil) or Haematocoocus Pluvialls (Haematococcus pluvialis or lacustris).
7., by the application of the natural lipoclastic described in claim 1 or 2, it is characterized in that: calculate according to the male of 60kg body weight, described natural carotenoid and the general >5.0mg of oxidized derivatives Day feeding amount thereof.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041595A (en) * | 2017-12-05 | 2018-05-18 | 杭州鑫伟低碳技术研发有限公司 | A kind of infertile composition of auxiliary treatment |
| CN108719591A (en) * | 2017-04-16 | 2018-11-02 | 韦智 | A kind of feed formula that can improve animal sperm active function |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6054491A (en) * | 1996-03-27 | 2000-04-25 | Astacarotene Ab | Agent for increasing the production of/in breeding and production mammals |
| US6410602B1 (en) * | 1997-12-09 | 2002-06-25 | Astacarotene Ab | Method of increasing the production and improving the quality of semen |
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2014
- 2014-03-19 CN CN201410102560.8A patent/CN104922098A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6054491A (en) * | 1996-03-27 | 2000-04-25 | Astacarotene Ab | Agent for increasing the production of/in breeding and production mammals |
| US6410602B1 (en) * | 1997-12-09 | 2002-06-25 | Astacarotene Ab | Method of increasing the production and improving the quality of semen |
Non-Patent Citations (1)
| Title |
|---|
| F. H. COMHAIRE ET AL: "Combined conventional/antioxidant "Astaxanthin" treatment for male infertility: a double blind, randomized trial", 《ASIAN J ANDROL》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108719591A (en) * | 2017-04-16 | 2018-11-02 | 韦智 | A kind of feed formula that can improve animal sperm active function |
| CN108041595A (en) * | 2017-12-05 | 2018-05-18 | 杭州鑫伟低碳技术研发有限公司 | A kind of infertile composition of auxiliary treatment |
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Application publication date: 20150923 |
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| RJ01 | Rejection of invention patent application after publication |