CN104916200A - An application method of the sixth finger in histology and embryology experiment teaching - Google Patents
An application method of the sixth finger in histology and embryology experiment teaching Download PDFInfo
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- CN104916200A CN104916200A CN201510320129.5A CN201510320129A CN104916200A CN 104916200 A CN104916200 A CN 104916200A CN 201510320129 A CN201510320129 A CN 201510320129A CN 104916200 A CN104916200 A CN 104916200A
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- histology
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
Abstract
The invention discloses an application method of the sixth finger in histology and embryology experiment teaching. The sixth finger can be obtained through operation; after the fixation of a fixation solution of a certain concentration, the sixth finger is subjected to dehydration, hyalinization, waxdipping, embedding, slicing, dyeing and slice packaging in a certain manner; and finally, the sixth finger is used for observation of epithelial tissue, connective tissue, cartilage and bone and muscular tissue in histology and embryology experiment teaching, so that the problem that it is not easy to obtain a normal human tissue sample in the histology and embryology experiment teaching is solved, and effective utilization of experiment resources is expanded. Besides, the sixth finger is easily available, can be obtained from an operating room of the department of orthopaedics, can be obtained timely, and can be applied to the scientific research of human tissue engineering; the cell culture technique is adopted to separate epithelial cells, adipose cells and osteoblasts of the sixth finger, and thus the obtained cells are closer to real cell experiment; and the sixth finger is used for scientific experiment researches.
Description
Technical field
The invention belongs to bioconversion medium technical field, particularly relate to a kind of 6th and refer to the application process in Histology and Embryology experimental teaching.
Background technology
Human congenital finger skeleton deformity is a kind of common hereditary disease.Human embryos are grown and from 4th week end, are occurred that two to protuberance at abdomen lateral wall, i.e. anterior limb bud and hindlimb bud.Form normal four limbs through a series of growth course, cause cacomelia because genetic defect can make dysmelia, fall ill to refer to comparatively general more.6th refers to have dactylus, nail equally, also can be movable together along with the other the five fingers, but this can bring certain impact to the growth of child.So most father and mother can require operative treatment.Therapic opportunity pure polydactyly, particularly ulnar side pure polydactyly, do not have metacarpal bone and the superfluous life of metacarpophalangeal joints, should complete the treatment of polydactyly corrective procedure within the baby 3-6 month.Compositeness polydactyly, as more the many fingers of oar side compositeness and central authorities refer to and refer to, the polydactyly corrective procedure time can postpone, but must strive for completing before 2 years old, and take comfort early psychologically in order to the head of a family, infant does not also have mental handicape.Based on this, the 6th operation referred to not only relieves the physiology of patient and psychological illness, and meanwhile, the postoperative 6th refers to but provide good resource for medical experiment instruction and scientific research.Due to the 6th refer to easily acquired, and with the consistance of normal human tissue structure, for the experimental teaching of Histology and Embryology and Students ' Learning Basic Medical Curriculum provide tissue-derived, be a kind of experimental technique being applied to Histology and Embryology be worthy to be popularized.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of 6th to refer to the application process in Histology and Embryology experimental teaching, is intended to solve tissue-derived difficulty in Histology and Embryology experimental teaching, for the normal institutional framework of observation of students provides abundant tissue-derived.
The embodiment of the present invention realizes like this, a kind of by paraffin section technology, refer to through a series of fixing by the obtained the 6th, dehydration, transparent, embedding, section, the technology such as dyeing, obtain the paraffin section referred to, for the stratified squamous epithelium of the Germicidal efficacy epithelial tissue of Histology and Embryology, loose connective tissue, the structure of the bony segment flesh of finger, observe even the structure of cartilage and bone, this the 6th section referred to, solve in Histology and Embryology experiment epithelial tissue in the four large tissues observing normal organization, connective tissue, three large problems of musculature.
Another object of the embodiment of the present invention is the tissue-derived problem providing fresh bone tissue and adipose tissue, solves the problem of the original cuiture of cell chulture good in the experiment of organizational project.
The embodiment of the present invention realizes like this, pass through cell culture technology, refer to that by the obtain the 6th cell, the adipocyte of the bone required for tissue separation carry out original cuiture, the cell of separation and purification is carried out inoculate, go down to posterity, finally carry out observing and record under inverted microscope, for the 6th refers to that application in organizational project provides good tissue-derived.
Provided by the invention 6th refers to the application process in Histology and Embryology experimental teaching, supplement problem tissue-derived in experimental teaching, for the autonomous making paraffin section of student provides material source with the normal institutional framework of observation, not only be beneficial to the experimental teaching of teaching, improve quality of instruction, also for the autonomous learning of student provide convenience tissue-derived.From teaching and the Students ' Learning two aspect benefit all to some extent of teacher, this kind of method should be worthy of promotion and application.
Accompanying drawing explanation
Fig. 1 is the application process process flow diagram of the 6th finger in Histology and Embryology experimental teaching that the embodiment of the present invention provides;
Fig. 2 is that schematic diagram is shown in the 6th application of finger in Histology and Embryology experimental teaching that the embodiment of the present invention provides.
In figure: (a) epithelial tissue, stratified squamous epithelium; (b) connective tissue, loose connective tissue; (c) connective tissue, hyaline cartilage; (d) nerve fiber, corpuscula lamellosa; (e) nail; (f) sweat gland.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As depicted in figs. 1 and 2, the 6th of the embodiment of the present invention refers to that the application process in Histology and Embryology experimental teaching comprises the following steps: based on this, my seminar combines my institute's orthopaedics center, by the patient of suffered from Hexadactyly operation, register, six fingering row of partial surgical are preserved and are used for the experimental teaching of Histology and Embryology.Experiment flow: 1. Post operation refers to put in 10% formalin immobile liquid fix 48 hours by the 6th rapidly; 2. running water 12-24 hour immediately, removes immobile liquid; 3. the tissue block after running water is put into successively each 8 hours of the ethanol of 70%, 80%, 90%, 95%, in absolute ethyl alcohol 4 hours, fully slough the moisture in tissue block; 4. refer to put into the paraffin (56-60 DEG C) melted by the transparent good 6th, to immerse gradually in paraffin about 1.5 hours; 5. poured into by the paraffin melted in metal embedding frame, then the tissue block soaked into is put into the central authorities of waxdip frame, after cooling, the 6th refers to will be embedded in paraffin mass; 6. embedded tissue block is fixed on wooden unit, then is placed on microtome, be cut into 4 μm of slabs, put into warm water and launch, then to be attached on microslide and to put into baking box and dry.7. take out the microslide dried, put into dimethylbenzene 5-6 minute, slough paraffin, and then section is put into 100%, 95%, 90%, 80%, 70% ethanol each 4 minutes successively.Take off dimethylbenzene.8. hematoxylin dyeing 10 minutes is put in section, nucleus basophilla is dyed to hyacinthine, put into 0.5% acidic alcohol differentiation about 20 seconds again, running water 20 minutes, immerse again in 0.5%eosin and dye 7 minutes, tenuigenin acidophilia dyes pink, the washing several seconds washes away loose colour, use 70%, 80%, 90%, 95%, 100%, 100% ethanol dehydration more each 2 minutes, transparent 8 minutes of dimethylbenzene, after carrying out mounting with canada balsam, may be used for the microtexture that educational observation refers to.
6th paraffin section referred to can do crosscut and the rip cutting of whole, and for the experimental teaching of Histology and Embryology, because this kind of sample easily obtains, institutional framework is consistent with the structure normally pointed, and may be used for the experimental teaching of Histology and Embryology; 6th acquisition referred to is easy, can obtain, obtain in time, may be used for the scientific research of people's organizational project from bone surgery room, and be separated the epithelial cell of people, adipocyte and Gegenbaur's cell, study for scientific experiment.Refer to be soaked in rapidly in PBS liquid by the postoperative 6th, after the skin that 6th refers to is separated with hypodermis, strip out phalanges, reject periosteum, blood vessel and connective tissue, be placed with dual anti-(penicillin 100U/ml, streptomysin 100pg/m1) Hanks liquid in embathe for several times, osteocomma is put into penicillin bottle and is cut into 1-4mm
3fritter after, adding the II Collagenase Type solution (Hanks liquid prepare, 37 DEG C of water-baths, 2 hours rearmounted 4 DEG C of refrigerator overnight) of 1.5mg/ml, is 2:1 with the volume ratio of tissue block.37 DEG C of water-bath joltings digest 20 minutes, and period uses hand bottle frequently, discards suspension, rejoin collagenase solution digestion, collect upper suspension after 45 minutes, three times so repeatedly, concentrate all upper suspensions, 1000 revs/min 5 minutes centrifugal, outwell supernatant, the same rotating speed centrifuge washing of precipitation Hanks liquid three times, with the DMEM complete culture solution suspendible cell of 2ml containing 20%FCS, penicillin 100U/ml, streptomysin 100 μ g/ml, residue osteocomma discards, and tally counting cells, can obtain cell and be about lx10
6individual/ml.Thus provide tissue and cell derived for the primary cell of cell chulture in organizational project.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. one kind the 6th refers to the application process in Histology and Embryology experimental teaching, it is characterized in that, 6th refers to that the application process in Histology and Embryology experimental teaching comprises: referred to by operation acquisition the 6th, fixing through certain concentration, dehydration, transparent, waxdip, embedding, section, dyeing and mounting, finally for the observation of Histology and Embryology experimental teaching epithelial tissue, connective tissue, cartilage and bone and musculature.
2. the as claimed in claim 1 6th refers to the application process in Histology and Embryology experimental teaching, it is characterized in that, the 6th refers to that the concrete steps of the application process in Histology and Embryology experimental teaching are as follows:
Step one, Post operation refers to put in 10% formalin immobile liquid fix 48 hours by the 6th rapidly;
Step 2, immediately running water 12-24 hour, remove immobile liquid;
Step 3, puts into each 8 hours of the ethanol of 70%, 80%, 90%, 95% successively by the tissue block after running water, in absolute ethyl alcohol 4 hours, fully sloughs the moisture in tissue block;
Step 4, refers to put into the paraffin 56-60 DEG C melted by the transparent good 6th, to immerse gradually in paraffin about 1.5 hours;
Step 5, pours into the paraffin melted in metal embedding frame, then the tissue block soaked into is put into the central authorities of waxdip frame, and after cooling, the 6th refers to will be embedded in paraffin mass;
Step 6, is fixed on embedded tissue block on wooden unit, then is placed on microtome, is cut into 4 μm of slabs, puts into warm water and launch, then to be attached on microslide and to put into baking box and dry;
Step 7, takes out the microslide dried, puts into dimethylbenzene 5-6 minute, slough paraffin, and then section is put into 100%, 95%, 90%, 80%, 70% ethanol each 4 minutes successively, takes off dimethylbenzene;
Step 8, hematoxylin dyeing 10 minutes is put in section, and nucleus basophilla is dyed to hyacinthine, then puts into 0.5% acidic alcohol differentiation 20 seconds, running water 20 minutes, immerse in 0.5%eosin again and dye 7 minutes, tenuigenin acidophilia dyes pink, and the washing several seconds washes away loose colour, use 70%, 80%, 90%, 95%, 100%, 100% ethanol dehydration more each 2 minutes, transparent 8 minutes of dimethylbenzene, after carrying out mounting with natural gum, for the microtexture that educational observation refers to.
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Application publication date: 20150916 |