CN104911818A - Preparation method for discrete-stated PEDOT three-dimensional fiber scaffold - Google Patents
Preparation method for discrete-stated PEDOT three-dimensional fiber scaffold Download PDFInfo
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- CN104911818A CN104911818A CN201510263753.6A CN201510263753A CN104911818A CN 104911818 A CN104911818 A CN 104911818A CN 201510263753 A CN201510263753 A CN 201510263753A CN 104911818 A CN104911818 A CN 104911818A
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Abstract
The invention discloses a preparation method for a discrete-stated PEDOT three-dimensional fiber scaffold. The preparation method for the discrete-stated PEDOT three-dimensional fiber scaffold comprises the following steps that a discrete-stated electrospinning fiber scaffold is prepared; the discrete-stated electrospinning fiber scaffold is put into EDOT solution to contact fully, and then ferric trichloride solution is added to conduct ultrasonic reaction; the discrete-stated electrospinning fiber scaffold is soaked and washed by water after the reaction is finished, and then the discrete-stated PEDOT three-dimensional fiber scaffold can be obtained by freezing drying, the preparation method for the discrete-stated PEDOT three-dimensional fiber scaffold has the advantages that the electrostatic spinning technology is simple, and the cost is low; the prepared three-dimensional fiber scaffold is more easy to control.
Description
Technical field
The invention belongs to electrostatic spinning technique field, particularly relate to a kind of preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds.
Background technology
In recent years, along with the development of material science, the research report about nano material continues to increase, and especially the preparation and application research of nano-fiber material attracts widespread attention, and becomes one of the focus in investigation of materials field.Researcher develops many methods preparing nanofiber, and wherein, electrostatic spinning technique is the important means preparing nanofiber, and prepared fiber and extracellular matrix have similar structure, has the features such as great specific area and high porosity.
Current numerous research shows that electrospinning fibre builds suitable tissue engineering bracket thus forms cytoskeleton complex in vitro, and then transplants damage location in body and carry out one of tissue repair, the optimal selection promoting regeneration.If timbering material wants the application prospect being lifted at field of tissue engineering technology, improve support porosity and the functional fiber timbering material making it possess inner multiple unit mutually through is very wise move, because the support of functionalization significantly can improve the regeneration function of tissue.
Although researcher has prepared electrospinning fibre timbering material miscellaneous by change different parameters and technique.But, these supports are plane electrospinning fibre membrane structure mainly, arrange too tight between fiber and fiber, the growing space on surface can only be provided as cell culturing rack material, cell is difficult to enter into internal stent to carry out growing and breeding, and this tunica fibrosa with surface structure strongly limit its application at biomedical engineering field.
Summary of the invention
The present invention is directed in prior art and there is the defects such as technique is loaded down with trivial details, fibre three-dimensional degree is inadequate, cell compatibility is poor, provide a kind of preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds, this discrete Three Dimensional Fiber Scaffolds effectively can improve cell loading and the natural survival in vitro microenvironment of class of support.
In order to achieve the above object, the invention discloses a kind of preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds, comprise following concrete steps:
Step 1: be dissolved in by spinning material in the mixed solution of carrene and DMF, is stirred to solution clarification, then the bubble in centrifugal removing solution;
Step 2: carry out electrostatic spinning process again, obtains nanometer Three Dimensional Fiber Scaffolds;
Step 3: configure isopyknic liquor ferri trichloridi and 3 respectively, 4-ethene dioxythiophene (EDOT) solution, first being soaked in EDOT solution by nanometer Three Dimensional Fiber Scaffolds obtained in step 2 makes it fully contact, then is placed in the ultrasonic process of liquor ferri trichloridi;
Step 4: after reaction terminates, is soaked in water and rinses and after freeze drying, namely obtain discrete PEDOT Three Dimensional Fiber Scaffolds.
Preferably, in described step 1, spinning material is any one in PLA, Poly(D,L-lactide-co-glycolide, polyvinylpyrrolidone, polyacrylonitrile, polycaprolactone, carrene and N, the mixed volume of N-dimethylformamide is than being (8 ~ 2): 2, and spinning material accounts for 5% ~ 10% of mixture system gross mass.
Preferably, in described step 2, electrospinning condition is: spray silk syringe needle internal diameter is 0.22mm, and voltage 12kV, receiving range 10cm, fltting speed is 1.5mL/h.
Preferably, in described step 3, in liquor ferri trichloridi, solvent is one or more mixing in water, ether, acetone and carrene, and liquor ferri trichloridi concentration is 0.1 ~ 0.5g/mL; In EDOT solution, solvent is one or more mixing in water, ether, acetone and carrene, and EDOT solution concentration is 0.1 ~ 0.5g/mL.
Preferably, in described step 3, sonification power is 100W, and frequency is 50KHz.
Preferably, in described step 4, freeze drying adopts freeze drier, dry more than 48 hours.
Preferably, in described step 4, the fibre diameter of prepared Three Dimensional Fiber Scaffolds 1.0 ~ 2.5 microns, the aperture size of support is between 10 ~ 150 microns, and the PEDOT thickness of fiber surface is about 40 ~ 100 nanometers.
Compared with prior art, advantage of the present invention is:
(1) preparation method of the present invention is that cell chulture provides feasible Method and kit for.
(2) electrostatic spinning process of the present invention is simple, with low cost.
(3) three-dimensional rack pattern of the present invention is more easy to control.
Accompanying drawing explanation
Fig. 1 is the discrete PLA three-dimensional fiber scanning electron microscope (SEM) photograph that the present invention obtains.
Fig. 2 is the discrete PEDOT Three Dimensional Fiber Scaffolds pictorial diagram that the present invention obtains.
Detailed description of the invention
Below in conjunction with specific embodiment, illustrate the present invention further.
Embodiment 1 is that raw material makes discrete PEDOT Three Dimensional Fiber Scaffolds with PLA
1. be dissolved in by the PLA of 1.05 grams in carrene and DMF (8:2) mixed solution, mass fraction 5%, magnetic agitation is homogeneous to solution clarification, then under 1000 turns centrifugal 1 minute, the bubble in removing solution.
2. on the electric spinning equipment (its structure and operating process thereof are shown in that application number is 201310026760.5 patents) of self-service dress, carry out electrostatic spinning process, the discrete PLA three-dimensional fiber scanning electron microscope (SEM) photograph that collection obtains as shown in Figure 1; Electrospinning condition: positive pole high-pressure injector head employing internal diameter is the spray silk syringe needle of 0.22mm, and voltage 10kV, receiving range is 10cm, and fltting speed is 1.5ml/h.
3. configure the ethanolic solution of isopyknic 0.1g/mL ferric trichloride and the ethanolic solution of 0.1g/mL EDOT respectively; PLA Three Dimensional Fiber Scaffolds obtained in step 2 is soaked in the ultrasonic process of ethanolic solution 30 minutes being placed in ferric trichloride after making it fully contact in the ethanolic solution of EDOT again.
4. after reaction terminates, the three-dimensional rack in step 3 to be transferred in deionized water soaking and washing 2 hours, clean 3-5 time, remove Fe
2+, Fe
3+in remaining ion.Then put into-80 DEG C of refrigerator overnight freezing, finally use freeze drier dry 48 hours, be i.e. obtained discrete PEDOT Three Dimensional Fiber Scaffolds as shown in Figure 2.
Embodiment 2 is that raw material makes discrete PEDOT Three Dimensional Fiber Scaffolds with polyacrylonitrile
1. be dissolved in by the polyacrylonitrile of 1.2 grams in carrene and DMF (6:2) mixed solution, mass fraction 7%, magnetic agitation is homogeneous to solution clarification, then under 1000 turns centrifugal 1 minute, the bubble in removing solution.
2. on the electric spinning equipment (its structure and operating process thereof are shown in that application number is 201310026760.5 patents) of self-service dress, carry out electrostatic spinning process, electrospinning condition: positive pole high-pressure injector head employing internal diameter is the spray silk syringe needle of 0.22mm, voltage 12kV, receiving range is 10cm, and fltting speed is 1.0ml/h.
3. configure the acetone soln of isopyknic 0.3g/mL ferric trichloride and the acetone soln of 0.3g/mL EDOT respectively; Polyacrylonitrile Three Dimensional Fiber Scaffolds obtained in step 2 is soaked in ultrasonic 30 minutes of the acetone soln being placed in ferric trichloride after making it fully contact in the acetone soln of EDOT again.
4. after reaction terminates, the Three Dimensional Fiber Scaffolds in step 3 to be transferred in deionized water soaking and washing 2 hours, repeats 3-5 time, remove Fe
2+, Fe
3+in remaining ion.Then put into-80 DEG C of refrigerator overnight freezing, finally use freeze drier dry 48 hours, be i.e. obtained discrete PEDOT Three Dimensional Fiber Scaffolds.
Embodiment 3 is that raw material makes discrete PEDOT Three Dimensional Fiber Scaffolds with polycaprolactone
1. be dissolved in by the polycaprolactone of 1.19 grams in carrene and DMF (6:2) mixed solution, mass fraction is 9%, and magnetic agitation is homogeneous to solution clarification, then under 1000 turns centrifugal 1 minute, the bubble in removing solution.
2. on the electric spinning equipment (its structure and operating process thereof are shown in that application number is 201310026760.5 patents) of self-service dress, carry out electrostatic spinning process, electrospinning condition: positive pole high-pressure injector head employing internal diameter is the spray silk syringe needle of 0.22mm, voltage 15kV, receiving range is 10cm, and fltting speed is 0.8ml/h.
3. configure the dichloromethane solution of isopyknic 0.5g/mL ferric trichloride and the dichloromethane solution of 0.5g/mL EDOT respectively; Polycaprolactone Three Dimensional Fiber Scaffolds obtained in step 2 is soaked in ultrasonic 30 minutes of the dichloromethane solution being placed in ferric trichloride after making it fully contact in the dichloromethane solution of EDOT again.
4. after reaction terminates, Three Dimensional Fiber Scaffolds to be forwarded in water to soaking and washing 2 hours, repeats 3-5 time, remove Fe
2+, Fe
3+in remaining ion.Then put into-80 DEG C of refrigerator overnight freezing, finally use freeze drier freeze-drying 48 hours, be i.e. obtained discrete PEDOT Three Dimensional Fiber Scaffolds.
Claims (7)
1. a preparation method for discrete PEDOT Three Dimensional Fiber Scaffolds, is characterized in that, comprises following concrete steps:
Step 1: be dissolved in by spinning material in the mixed solution of carrene and DMF, is stirred to solution clarification, then the bubble in centrifugal removing solution;
Step 2: carry out electrostatic spinning process again, obtains nanometer Three Dimensional Fiber Scaffolds;
Step 3: configure isopyknic liquor ferri trichloridi and EDOT solution respectively, is first soaked in nanometer Three Dimensional Fiber Scaffolds obtained in step 2 in EDOT solution and makes it fully contact, then be placed in the ultrasonic process of liquor ferri trichloridi;
Step 4: after reaction terminates, is soaked in water and rinses and after freeze drying, namely obtain discrete PEDOT Three Dimensional Fiber Scaffolds.
2. the preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds as claimed in claim 1, it is characterized in that, in step 1, spinning material is any one in PLA, Poly(D,L-lactide-co-glycolide, polyvinylpyrrolidone, polyacrylonitrile, polycaprolactone.
3. the preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds as claimed in claim 1, it is characterized in that, in step 1, carrene and N, the mixed volume of N-dimethylformamide is than being (8 ~ 2): 2, and spinning material accounts for 5% ~ 10% of mixture system gross mass.
4. the preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds as claimed in claim 1, it is characterized in that, in step 2, electrospinning condition is: spray silk syringe needle internal diameter is 0.22mm, and voltage 12kV, receiving range 10cm, fltting speed is 1.5mL/h.
5. the preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds as claimed in claim 1, it is characterized in that, in step 3, in liquor ferri trichloridi, solvent is one or more mixing in water, ether, acetone and carrene, and liquor ferri trichloridi concentration is 0.1 ~ 0.5g/mL; In EDOT solution, solvent is one or more mixing in water, ether, acetone and carrene, and EDOT solution concentration is 0.1 ~ 0.5g/mL.
6. the preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds as claimed in claim 1, it is characterized in that, in step 3, sonification power is 100W, and frequency is 50KHz.
7. the preparation method of discrete PEDOT Three Dimensional Fiber Scaffolds as claimed in claim 1, is characterized in that, in step 4, freeze drying adopts freeze drier, dry more than 48 hours.
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Cited By (2)
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| CN109306539A (en) * | 2017-07-28 | 2019-02-05 | 南京理工大学 | A kind of 3D conduction cell culturing bracket and preparation method thereof |
| CN115094529A (en) * | 2022-07-11 | 2022-09-23 | 吉林大学第一医院 | Porous orientation PLGA electrostatic spinning fiber and preparation method thereof |
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| CN115094529A (en) * | 2022-07-11 | 2022-09-23 | 吉林大学第一医院 | Porous orientation PLGA electrostatic spinning fiber and preparation method thereof |
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