CN104911345A - Mineral microorganism screening device and microorganism screening method - Google Patents
Mineral microorganism screening device and microorganism screening method Download PDFInfo
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- CN104911345A CN104911345A CN201510205731.4A CN201510205731A CN104911345A CN 104911345 A CN104911345 A CN 104911345A CN 201510205731 A CN201510205731 A CN 201510205731A CN 104911345 A CN104911345 A CN 104911345A
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- microorganism
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- leaching
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a mineral microorganism screening device including a gas inlet, an overflow port and a feed port, the gas inlet and the feed port are arranged at the lower part of the device, the overflow port is arranged at the upper part of the device, and a graphite felt is used as a bed layer separator plate of a fixed bed layer of the device. The invention also discloses a microorganism screening method of acidophilic oxidation sulfide ore. By use of the graphite felt as the bed layer separator plate, the air concentration in a bacterial culture solution is effectively improved, and the method has the advantages of short screening cycle, reduction of mineral particle friction damage to bacteria, and high cultured microorganism activity, by use of sea water and fresh water for mixed leaching of ocean sediment sulfides and useful metals in ocean manganese nodules, compared with microorganism leaching in the prior art, leaching fresh water usage is reduced, and the practicability is improved.
Description
Technical field
The present invention relates to microbe to screen and leaching field, ore deposit, particularly, the present invention relates to screening plant and the screening method of leaching microbacteria in a kind of oceanic deposit sulfide.
Background technology
Biological metallurgy is the very potential novel metallurgical technique of one, have that less investment, energy consumption are low, reagent consumption less and the advantage such as process low grade ore.The microorganism of current participation microbial metallurgy mainly contains following several: thiobacillus ferrooxidant, thiobacillus thioparus, thiobacillus thiooxidans, deamination thiobacillus and some heterotrophic bacteriums etc.Although these bacteriums may be used for the metallic ore processing low-grade and difficult choosing, this bacterioid is poor for seawater tolerance level, is restricted in the utilization of the large midocean multi-metallic minerals of process.
The R&D institution being engaged in bio-oxidation process research is at present not less than ten, successively there are Institute of Microorganism, Academia Sinica, Shaanxi geology and minerals bureau of Ministry of Geology and Mineral Resources and comprise tens units of Central South University and carried out the research of this respect, simultaneously also in the tolerance and bacteria domestication of different kinds of ions, achieve gratifying achievement, but for screening can tolerate seawater and the solution that do not had of the efficient bacteria screening problem leaching mineral.This area most of ore-leaching bacteria used is mostly direct to be sampled from the acidic mine water in metal sulfide and colliery, and because bacterium grows and long-term domestication under physical environment, itself is poor for seawater tolerance, limits it and soaks application in ore deposit at ocean mineral.
Summary of the invention
The object of the present invention is to provide a kind of bacteria screening device, another object of the present invention is the problem for solving the mining microbial culture of above-mentioned existing leaching, provides a kind of acidophilic microorganism cultural method.
Device provided by the invention comprises inlet mouth, overflow port and opening for feed, and described inlet mouth and opening for feed are positioned at the bottom of described device, and described overflow port is positioned at the top of described device, and the fixed bed of described device take graphite felt as bed dividing plate.
Preferably, the working temperature of described fixed bed is 0-55 degree Celsius.
Present invention also offers the method utilizing said apparatus to carry out microorganism extraction, comprising:
1) form upwelling using microbial culture medium in the lump as bacteria screening solution and air, upwelling is through fixed bed, and overflow is discharged;
2) by step 1) overflow solution in Continuous Flow mode, from bottom feed mouth import, when nutrient solution becomes reddish-brown from light green, overflowing liquid is for filtering out microorganism.
Preferably, ore fixed bed arranged is for leaching pyrite, chalcopyrite, ocean sulfuration settling and/or ocean manganese nodule.
Preferably, the pH of described microbial culture medium is 2.0-3.0.
Preferably, the substratum of described microbial culture medium is the 9K substratum adding about 5% ~ 15% seawater.According to screening process of the present invention, wherein microorganism is using inorganic salt and oceanic deposit mineral medium sulphide content as energy derive, and described inorganic salt are 9K substratum, and its main component is as follows: (NH
4)
2sO
41.0 ~ 3.0g/L, KCl0.05 ~ 0.2g/L, MgSO
47H
2o 0.2 ~ 1.0g/L, K
2hPO
40.3 ~ 1.0g/L, Ca (NO
3)
20.005 ~ 0.015g/L, FeSO
47H
2o 20 ~ 50g/L.
The microorganism of sifting out in the present invention, has the characteristic of one or more in common bacteria thiobacillus thiooxidans, thiobacillus thioparus, deamination thiobacillus and thiobacillus ferrooxidant and good tolerance.
Use the screening method of leaching microbacteria in above-mentioned oceanic deposit sulfide, the leaching microbacteria of tolerance seawater can be filtered out, shorten the screening microorganism time simultaneously.The present invention adopts new fixed bed pattern, and leach the bacterium of ore under can be used in of filtering out tolerates seawater situation, more existing leaching microbacteria can leach ocean mineral while tolerance seawater, has obvious advantage.
The present invention is based on bacteria live and growing environment, adopt graphite felt fixed bed bacteria screening device, screening can leach the microorganism of mineral; This device effectively can increase bacterial growth ambient oxygen concentration, promote bacterial growth, decreases mineral simultaneously and mutually collides in the solution and injure bacterium.This device can screening bacterium while, for the culture apparatus of bacterium before follow-up leaching ore deposit.
Accompanying drawing explanation
Graphite felt fixed bed bacteria screening device schematic diagram (rising streaming) of Fig. 1, the embodiment of the present invention.
Accompanying drawing identifies:
1, inlet mouth; 2, opening for feed; 3, overflow port; 4, fixed bed.
Embodiment
See accompanying drawing 1, the device that the present invention screens microorganism comprises inlet mouth 1, overflow port 3 and opening for feed 2, described inlet mouth 1 and opening for feed 2 are positioned at the bottom of described device, and described overflow port 3 is positioned at the top of described device, and the fixed bed 4 of described device take graphite felt as bed dividing plate.The working temperature of described fixed bed 4 is 0-55 degree Celsius.
The following is the embodiment utilizing aforementioned means to screen microorganism.
Embodiment 1
Certain seabed many metallic coppers sulphide ores, its main component is as follows: SiO
254.23%, Cu 4.24%, Fe10.52%, S, 15.41%, Al3.86%, mineral composition: metalliferous mineral is mainly pyrite and chalcopyrite, quartz and manosil AS mineral are main gangue mineral.
Take a certain amount of sample ore, levigate extremely-200 orders account for 95%, then by levigate sample ore with 20% solid-liquid ratio add containing 10% seawater without in Fe9K substratum, inoculate the bacterium of sifting out from oceanic deposit sulfide, put at 30 DEG C and shake the concussion shaking table reaction 10d that speed is 160r/min, the leaching yield of final copper reaches 90%.
Embodiment 2
Ocean manganese nodule, its chemical composition is: Mn21.24%, Fe 17.4%, SiO
244.41%, Co 0.26%, Ni0.87%, Cu0.89%.Mineral composition: metalliferous mineral is mainly manganite, Co, Ni, Cu are present in manganese ore with isomorphism form simultaneously, and quartz is main gangue mineral.
The sulphur ore in certain mine, its main component is: S 48.68%, Fe 42.66%, SiO
22.44%.Mineral composition: metalliferous mineral is mainly pyrite, quartz is main gangue mineral.
Take pyrolusite and the pyrite sample ore being crushed to-1mm in proportion, add in iron-free 9K substratum, solid-to-liquid ratio is 30%, inoculate the bacterium of sifting out from oceanic deposit sulfide, pH is transferred to 2, puts into shake the concussion shaking table that speed is 160r/min and react 24h at 30 DEG C, and the leaching yield of manganese is 98%, cobalt leaching yield 90%, nickel leaching yield reach 95%, copper leaching rate is 82%.
Claims (6)
1. one kind is screened the device of microorganism in mineral, comprise inlet mouth, overflow port and opening for feed, it is characterized in that described inlet mouth and opening for feed are positioned at the bottom of described device, described overflow port is positioned at the top of described device, and the fixed bed of described device take graphite felt as bed dividing plate.
2. device according to claim 1, is characterized in that, the working temperature of described fixed bed is 0-55 degree Celsius.
3. utilize the arbitrary described device of claim 1 ~ 2 to carry out the method for microbe to screen, comprising:
1) form upwelling using microbial culture medium in the lump as bacteria screening solution and air, upwelling is through fixed bed, and overflow is discharged;
2) by step 1) overflow solution in Continuous Flow mode, from bottom feed mouth import, when nutrient solution becomes reddish-brown from light green, overflowing liquid is the microorganism filtered out.
4. method according to claim 3, is characterized in that, the ore that fixed bed is arranged is for leaching pyrite, chalcopyrite, ocean sulfuration settling and/or ocean manganese nodule.
5. method according to claim 3, is characterized in that, the pH of described microbial culture medium is 2.0-3.0.
6. method according to claim 3, is characterized in that, the substratum of described microbial culture medium is the 9K substratum adding about 5% ~ 15% seawater.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510205731.4A CN104911345B (en) | 2015-04-27 | 2015-04-27 | The device of microorganism and the method for screening microorganism in a kind of screening mineral |
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|---|---|---|---|
| CN201510205731.4A CN104911345B (en) | 2015-04-27 | 2015-04-27 | The device of microorganism and the method for screening microorganism in a kind of screening mineral |
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| Publication Number | Publication Date |
|---|---|
| CN104911345A true CN104911345A (en) | 2015-09-16 |
| CN104911345B CN104911345B (en) | 2017-07-14 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000028099A1 (en) * | 1998-11-06 | 2000-05-18 | Bactech (Australia) Pty Ltd. | Improved reactor |
| CN201193227Y (en) * | 2008-03-19 | 2009-02-11 | 北京科技大学 | Device for quickly cultivating strain for soaking ore |
| CN101838737A (en) * | 2009-12-01 | 2010-09-22 | 西部矿业股份有限公司 | Method for leaching low-grade phosphate ore from ferrous oxide thiobacillus |
| CN103413958A (en) * | 2013-07-31 | 2013-11-27 | 中国科学院过程工程研究所 | Acidophilic microbe fuel cell and culture method of acidophilic microbes |
| CN103509946A (en) * | 2012-06-14 | 2014-01-15 | 中国科学院过程工程研究所 | Method for vulcanizing low-grade oxidized nickel ore with microbiological method |
-
2015
- 2015-04-27 CN CN201510205731.4A patent/CN104911345B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000028099A1 (en) * | 1998-11-06 | 2000-05-18 | Bactech (Australia) Pty Ltd. | Improved reactor |
| CN201193227Y (en) * | 2008-03-19 | 2009-02-11 | 北京科技大学 | Device for quickly cultivating strain for soaking ore |
| CN101838737A (en) * | 2009-12-01 | 2010-09-22 | 西部矿业股份有限公司 | Method for leaching low-grade phosphate ore from ferrous oxide thiobacillus |
| CN103509946A (en) * | 2012-06-14 | 2014-01-15 | 中国科学院过程工程研究所 | Method for vulcanizing low-grade oxidized nickel ore with microbiological method |
| CN103413958A (en) * | 2013-07-31 | 2013-11-27 | 中国科学院过程工程研究所 | Acidophilic microbe fuel cell and culture method of acidophilic microbes |
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| CN104911345B (en) | 2017-07-14 |
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Granted publication date: 20170714 Termination date: 20200427 |