CN104897590A - Method for quickly detecting content of petroleum hydrocarbon and thallus in oily sewage treatment system - Google Patents
Method for quickly detecting content of petroleum hydrocarbon and thallus in oily sewage treatment system Download PDFInfo
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- CN104897590A CN104897590A CN201510297486.4A CN201510297486A CN104897590A CN 104897590 A CN104897590 A CN 104897590A CN 201510297486 A CN201510297486 A CN 201510297486A CN 104897590 A CN104897590 A CN 104897590A
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Abstract
The invention relates to a method for quickly detecting content of petroleum hydrocarbon and thallus in an oily sewage treatment system. Under condition of different wavelength, the influences of petroleum hydrocarbon and bacteria on light absorption are different, and therefore, under the condition of a specific wavelength, the total absorption value of a suspension containing petroleum hydrocarbon and bacteria is the sum of the absorption values of petroleum hydrocarbon and bacteria. The method is combined with the characteristic that microorganisms and petroleum hydrocarbon have different absorption characteristics (especially, the maximum absorption peak has remarkable difference) in different wavelength. According to the invention, a simple linear equation in two unknowns is taken as a detection manner, and compared with conventional methods, the method disclosed by the invention is simple and easy and relatively accurate, thus providing a simple manner for providing the data of the content of petroleum hydrocarbon and thallus.
Description
Technical field
The present invention relates to the new method of a kind of quick detection oily water treatment system Petroleum Hydrocarbon and thalline content.
Background technology
Biological treatment is the biological chemistry action utilizing microorganism, is simple material, noxious material is converted into innocuous substance by the organic substance decomposing of complexity, and waste water is purified.The petroleum hydrocarbon contaminated research of Biochemical method starts from phase early 1990s, and the feature of, non-secondary pollution low, effective because of its expense, has become the focus of research.Petroleum hydrocarbon content and micro organism quantity are requisite important indicators in oily water treatment.
The method of traditional detection petroleum hydrocarbon utilizes carbon tetrachloride extraction out to detect absorbance the crude oil in oily sewage, takes time and effort; And the method detecting thalline content is separated by centrifugal method by thalline, then measure its OD600 value to analyze content of microorganisms, these work take time and effort.The Cleaning Principle of above-mentioned two kinds of methods is material and has certain absorption to different light, and its absorption characteristic (maximum absorption band and extinction characteristic) is different.Under different wave length condition, petroleum hydrocarbon is different with the impact of bacterium on light absorption.Therefore under a specific wavelength condition, the absorption value sum that the total absorption value of suspension containing petroleum hydrocarbon and bacterium is both petroleum hydrocarbon and bacterium.
This method has different absorption characteristic (especially its maximum absorption band has significant difference) at different wavelengths respectively in conjunction with microorganism and petroleum hydrocarbon, sets up the new method of the content of a kind of quick detection solution Petroleum Hydrocarbon and microorganism.
Summary of the invention
A kind of high flux is the object of the present invention is to provide to detect the new method of solution Petroleum Hydrocarbon and content of microorganisms fast.The method comprises the steps:
(1) in oily sewage, add bacterium to cultivate, get nutrient solution 2ml after 12h and carry out centrifugal, be separated and obtain bacterium within the scope of wavelength 200-600, measure OD value under different wave length, take wavelength as horizontal ordinate, OD value is ordinate drawing.
(3) extracted by supernatant phenixin after 2ml medium centrifugal, the oil extracted measures the OD value under different wave length within the scope of wavelength 200-600, with wavelength be horizontal ordinate, OD value be ordinate draw.
(4) be coefficient simulation formula z=ax+by with a, b, z is the OD value of mixed liquor, and x is the absorbance of petroleum hydrocarbon, and y is the OD value of thalline
(5) get nutrient solution and measure its OD value wavelength 400 and 600 times, obtaining data is z1 and z2, and the equation in simultaneous (4) can obtain x respectively, the value of y, i.e. petroleum hydrocarbon and the independent content of thalline
Advantage of the present invention is to build simple linear equation in two unknowns, solution Petroleum Hydrocarbon and bacteria content is detected fast based on this in conjunction with spectrophotometric method, simple compared with classic method, provide a kind of succinct mode for providing the data of petroleum hydrocarbon and thalline content.
Accompanying drawing explanation
Nothing.
Embodiment
This experimental technique is further illustrated below in conjunction with example:
Get different oil and bacterial suspension, the wavelength of employing 405 and 490nm detects respectively, found that, it is equation (1) and equation (2) that bacterium and the oil absorbance under two kinds of different wave lengths closes,
Containing in the bacterial cultures of oil, its solution detects under the wavelength of 405nm and 490nm, can obtain
with
and total absorbance value is the stack result of two kinds of heterogeneities, so equation (3) and (4) can be known,
Equation (1) and (2) are substituted into equation (3) can obtain:
Convert equation (4) to equation (6),
Equation (6) is substituted into equation (5), obtains equation (7),
If
for a,
for b, obtain equation (8),
Claims (3)
1. detect containing wastewater treatment system Petroleum Hydrocarbon and a thalline content method fast, the method comprises the steps:
(1) in oily sewage, add bacterium to cultivate, get nutrient solution 2ml after 12h and carry out centrifugal, be separated and obtain bacterium within the scope of wavelength 200-600, measure OD value under different wave length, take wavelength as horizontal ordinate, OD value is ordinate drawing.
(3) extracted by supernatant phenixin after 2ml medium centrifugal, the oil extracted measures the OD value under different wave length within the scope of wavelength 200-600, with wavelength be horizontal ordinate, OD value be ordinate draw.
(4) be coefficient simulation formula z=ax+by with a, b, z is the OD value of mixed liquor, and x is the absorbance of petroleum hydrocarbon, and y is the OD value of thalline
(5) get nutrient solution and measure its OD value wavelength 400 and 600 times, obtaining data is z1 and z2, and the equation in simultaneous (4) can obtain x respectively, the value of y, i.e. petroleum hydrocarbon and the independent content of thalline.
2. detect containing wastewater treatment system Petroleum Hydrocarbon and thalline content method fast according to the one described in claim 1, it is characterized in that the linear figure line obtaining petroleum hydrocarbon and thalline content respectively, in conjunction with both coefficient, obtain simple binary formula, thus spectrophotometric method can be used to detect the content of oily sewage Petroleum Hydrocarbon and bacterium fast.
3. detect fast containing wastewater treatment system Petroleum Hydrocarbon and thalline content method according to the one described in claim 1, it is characterized in that utilizing comparatively simple binary formula can obtain the independent content of petroleum hydrocarbon and thalline fast and more adequately.
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Cited By (5)
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| CN109564158A (en) * | 2016-08-04 | 2019-04-02 | 爱阔特株式会社 | Concentration of oil measuring device and concentration of oil measurement method |
| CN112098548A (en) * | 2020-09-02 | 2020-12-18 | 江汉大学 | Method for detecting purity of finished product of thio-organic arsenic |
| CN112345487A (en) * | 2019-08-08 | 2021-02-09 | 湖南中烟工业有限责任公司 | Method for judging concentration of monomer incense raw material solution based on near infrared spectrum technology |
| CN112557386A (en) * | 2020-12-14 | 2021-03-26 | 浙江海洋大学 | Identification method of microorganism capable of catalyzing alkane molecules in petroleum to produce long-chain fatty acid |
| CN113533683A (en) * | 2021-06-02 | 2021-10-22 | 广东新泓环境咨询有限公司 | Surface soil petroleum hydrocarbon pollution early warning method and system |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109564158A (en) * | 2016-08-04 | 2019-04-02 | 爱阔特株式会社 | Concentration of oil measuring device and concentration of oil measurement method |
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| CN112557386A (en) * | 2020-12-14 | 2021-03-26 | 浙江海洋大学 | Identification method of microorganism capable of catalyzing alkane molecules in petroleum to produce long-chain fatty acid |
| CN112557386B (en) * | 2020-12-14 | 2022-05-31 | 浙江海洋大学 | Identification method of microorganism capable of catalyzing alkane molecules in petroleum to produce long-chain fatty acid |
| CN113533683A (en) * | 2021-06-02 | 2021-10-22 | 广东新泓环境咨询有限公司 | Surface soil petroleum hydrocarbon pollution early warning method and system |
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Application publication date: 20150909 |