CN104887657A - Application of acetyl austroinulin in preparation of antitumor drugs - Google Patents
Application of acetyl austroinulin in preparation of antitumor drugs Download PDFInfo
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- CN104887657A CN104887657A CN201410084099.8A CN201410084099A CN104887657A CN 104887657 A CN104887657 A CN 104887657A CN 201410084099 A CN201410084099 A CN 201410084099A CN 104887657 A CN104887657 A CN 104887657A
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Abstract
The invention belongs to the chemical engineering and medical fields, and relates to an application of acetyl austroinulin in preparation of antitumor drugs. The invention provides the application of the acetyl austroinulin in preparation of the antitumor drugs. The invention also provides a method for inhibiting in-vitro tumor cell proliferation, namely a method adding the acetyl austroinulin into a tumor cell culture solution. The acetyl austroinulin is a natural product, and can significantly inhibit the tumor cell proliferation. The small-molecular compound is used as a new antitumor drug or an auxiliary component of antitumor drugs for development, has an obvious tumor inhibiting effect, is green and environmentally friendly, and provides a new way and method for treating and curing tumors.
Description
Technical field
The invention belongs to chemical field and field of medicaments, relate to acetyl group Australia euparin and preparing the application in antitumor drug.
Background technology
Tumor be body under various carcinogenic factor effect, some cells of local organization lose the normal regulation to its growth on gene level, cause its clonal abnormality hypertrophy and the abnormality that formed.The oncosis number of cases of China is quite huge, has data to show and accounts for 55% of whole world case load.
The impact of benign tumor on body is less, and main manifestations is local compression and obstructive symptom.Malignant tumor due to break up immature, growth is very fast, infiltrates the 26S Proteasome Structure and Function destroying organ, and can shift, thus serious on body impact.Malignant tumor, except can causing the local compression similar to above-mentioned benign tumor and obstructive symptom, also can have heating, intractable pain, can occur late period seriously becoming thin, the state of weak, anemia and cachexia.
The traditional Chinese medical science is thought, first the cause of cancer is equilibrium between yin and yang in human body, and histiocyte is under different carcinogenic factor long terms, and cell mutation causes.Cancerous tissue is also a part for human body in fact, and only at this imbalance between YIN and YANG of people, under generation-inhibition in five elements takes advantage of the prerequisite of insulting and changing, the immunosurveillance system of human body just can lose monitoring to it, develops as one pleases.Chinese herbal medicine can be saved one's life with QI and blood regulating, regulating YIN and YANG balance, maintenance normal vital signs; To train correction gas, to produce antibody, clear up " malicious source " and effect a permanent cure.Thus, Chinese herbal medicine extract becomes treatment tumor important directions.
Acetyl group Australia euparin is the extract of Folium Stevlae Rebaudianae.Folium Stevlae Rebaudianae is a kind of perennial catananche, and containing inulin glycosides in blade, its sugariness is 150 ~ 300 times of sucrose, and being a kind of natural sweetener of sugariness low in calories, high, is one of raw material of food and pharmaceutical industries.External being used for replaces glucide to do low caloric products, not only have no side effect, and some disease can be treated, as controlled diabetes, regulate blood pressure, vessel softening, reduction blood fat, blood sugar lowering, antibacterial hemostasis, analgesia, fat-reducing and beauty, YIN nourishing and the production of body fluid promoting, help digest, promote pancreas, spleen and stomach function and heat-clearing and toxic substances removing.Also effective in cure to obesity, heart disease, children's worm tooth etc., and enhance metabolism, the effect of strong health.
Acetyl group Australia euparin belongs to natural product, and bioavailability is high, Nature comparison is stable, has Clinical practice and is worth.Along with people's going deep into its chemistry and biology research, its molecular mechanism of action will be progressively clear and definite, and this will promote modifying for chemical structure and the structure activity study of this compounds further, and contribute to the medical value improving Folium Stevlae Rebaudianae.
Summary of the invention
The object of this invention is to provide the new medicinal usage of acetyl group Australia euparin.
On the one hand, the invention provides acetyl group Australia euparin and prepare the application in antitumor drug.Wherein, the English full name of acetyl group Australia Herba Lycopi is acetyl-austroinulin, and its structure is as shown in formula I.
Described antitumor drug can be medicines resistant to liver cancer, antiprostate cancer, medicament for resisting cervical cancer, also can be anti-leukemia medicine.
Experimental result shows, acetyl group Australia euparin can suppress multiple growth of cancer cells.Some of IC
50reach and micro-ly to rub (μm ol/L or μM) level.
This antitumor drug can be injection or tablet.
On the other hand, the invention provides a kind of method suppressing tumor cell in vitro to be bred, add in the culture fluid of tumor cell by acetyl group Australia euparin.
Described tumor cell can be hepatoma carcinoma cell, blood cell, cervical cancer cell, breast cancer cell or pancreatic cancer cell.In preference of the present invention, in said method, described tumor cell can be hepatoma carcinoma cell, stomach cancer cell or blood cell.
Better, described tumor cell is pc-3, PANC-1, K562, HGC or Hela.
The final concentration adding acetyl group Australia euparin is 1-100 μM.Such as, 1-5 μM, 1-10 μM, 2-12 μM, 2-20 μM, 2-30 μM, 2-60 μM, 14-28 μM, 15-30 μM, 10-50 μM, 5-60 μM, etc.
On the other hand, the invention provides a kind of antitumor drug, the active component of described antitumor drug is acetyl group Australia euparin.
In preference of the present invention, above-mentioned antineoplastic agent for tumor be hepatoma carcinoma cell, stomach cancer cell, cervical cancer cell or leukaemia.
The invention provides acetyl group Australia euparin and prepare the application in antitumor drug.Acetyl group Australia euparin is natural product, can the propagation of obvious inhibition tumor cell.Micromolecular compound of the present invention is developed as new antitumor drug or its auxiliary element, and tumor killing effect is obvious, environmental protection, provides a kind of new approach and means by for treatment and healing tumor.
Detailed description of the invention
Experimental technique:
1.. cell recovery
1) from liquid nitrogen container, take out cryopreservation tube, directly drop in 37 DEG C of warm water, and shake makes it melt as early as possible frequently.
2) from 37 DEG C of water-baths, take out cryopreservation tube, with suction pipe sucking-off cell suspension, inject centrifuge tube and add more than 10 times culture fluid, low-speed centrifugal after mixing, abandons supernatant, then repeats to wash once with culture fluid.
3), after suitably diluting with culture fluid, inoculated and cultured bottle, be placed on 37 DEG C of incubator quiescent culture, next day changes culture fluid, continues to cultivate.Go down to posterity when being cultured to finite concentration.PANC-1 cell culture is in the DMEM high glucose medium containing 10%Gibico hyclone, and SW620 human colon cancer cell is cultivated and trained liquid at Leibovitz ' s L-15, adds 10% hyclone; The cell culture such as K562, HGC, QGY, MCF-7, PC-3 are in 1640 culture medium containing 10% hyclone, SK-hep1, HeLa, HepG2, etc. cell culture containing in the DMEM high glucose medium of 10% hyclone, containing 100U/ml penicillin and 100 μ g/ml streptomycins in culture medium.
2.. passage is cultivated
Every day observation of cell growth situation, when cell grow in culture bottle about 90% converge time go down to posterity, about went down to posterity once every 2-4 days.One bottle goes down to posterity into three bottles, or a 25cm
2go down to posterity in a 75cm
2culture bottle in.Method:
1) with 1 × phosphate buffer washed cell once.
2) add the digestion of 2-3ml trypsinization liquid, be placed in 37 DEG C of incubator numbers minute.Pat Tissue Culture Flask with hands, make cell separation.
3) trypsinization is stopped with the suitable culture medium of the Gibico hyclone containing 10-15%.Cell is sub-packed in new culture bottle, continues to cultivate.
When suspension cell goes down to posterity, be directly collected in centrifuge tube centrifugal, abandon old culture medium.General one bottle of ratio going down to posterity into three bottles is sub-packed in new culture bottle, adds fresh culture and continues to cultivate.
3.. cell cryopreservation
1) get the cell tryptase protease digestion being cultured to exponential phase, to be collected in centrifuge tube and to count, centrifugal.
2) reject trypsin and old culture fluid, add the frozen culture fluid (containing 10%DMSO, 40%DMEM and 50%Gibico hyclone) configured, in cryopreserving liquid, the ultimate density of cell is 0.5-1 × 10
7/ ml.Blowing and beating gently with suction pipe makes cell even, and be then distributed in aseptic cryopreservation tube, often pipe adds 1-1.5ml.
3) cryopreservation tube is put into freezing storing box and put-80 DEG C of quick-freezings, move in liquid nitrogen container after 5 hours and preserve.
4.. medicine prepares:
Acetyl group Australia euparin is dissolved in DMSO (dimethyl sulfoxide), and the mother solution being mixed with 100mM or 50mM is for subsequent use.
Acetyl group Australia euparin can be bought from Rui Fensi bio tech ltd, Chengdu.
The structure of acetyl group Australia euparin is as shown in structural formula I.
The invention provides a kind of antitumor drug, the active component of described antitumor drug is acetyl group Australia euparin.Described tumor can be hepatoma carcinoma cell, stomach cancer cell, cervical cancer cell or blood cell.
Micromolecular compound of the present invention can adopt the preparation method of various routine to prepare.Such as, the method for artificial chemistry synthesis is adopted.
Utilize micromolecular compound of the present invention, by various conventional screening assays, can filter out and interactional material occur, as receptor, inhibitor or antagonist etc. with acetyl group Australia euparin.
The present invention and inhibitor, antagonist etc., when carrying out using (administration) on treating, can provide different effects.Usually, these materials can be formulated in nontoxic, inertia with in pharmaceutically acceptable aqueous carrier medium, wherein pH is about 5-8 usually, and preferably pH is about 6-8, and pH value can with being formulated the character of material and disease to be treated and changing to some extent.The pharmaceutical composition prepared can carry out administration by conventional route, comprising (but being not limited to): intramuscular, intraperitoneal, subcutaneous, Intradermal or topical.
For acetyl group Australia of the present invention euparin, can by itself and suitable pharmaceutically acceptable carrier coupling.This kind of pharmaceutical composition contains the compound and pharmaceutically acceptable carrier or excipient for the treatment of effective dose.This kind of carrier comprises (but being not limited to): saline, buffer, glucose, water, glycerol, ethanol and combination thereof.Pharmaceutical preparation should match with administering mode.Acetyl group Australia of the present invention euparin can be made into injection form, such as, be prepared by conventional method with normal saline or the aqueous solution containing glucose and other adjuvant.The pharmaceutical composition of such as Tablet and Capsula and so on, is prepared by conventional method.Pharmaceutical composition such as injection, solution, Tablet and Capsula should aseptically manufacture.The dosage of active component is treatment effective dose, such as every day about 1 microgram/kg body weight-Yue 5 mg/kg body weight.In addition, acetyl group Australia of the present invention euparin also can use together with other treatment agent.
When acetyl group Australia of the present invention euparin is used as medicine; the acetyl group Australia euparin for the treatment of effective dose can be applied to mammal; wherein this treatment effective dose is usually at least about 10 micrograms/kg body weight; and be in most of the cases no more than about 8 mg/kg body weight, preferably this dosage is about 10 micrograms/kg body weight-Yue 1 mg/kg body weight.Certainly, concrete dosage also should consider the factor such as route of administration, patient health situation, and these are all within skilled practitioners skill.
Embodiment 1MTS method measures acetyl group Australia euparin to the growth inhibited effect of attached tumor cells
SK-HEP1 cell (being called for short SK cell, purchased from Chinese Academy of Sciences's cell bank) 3 × 10
3/ hole is seeded to 96 orifice plates, cultivate within 24 hours, make it adherent after add acetyl group Australia euparin, if 6 Concentraton gradient, each concentration establishes 3 multiple holes.Cell at 37 DEG C, 5%CO
2cultivate after 72 hours under condition, outwell culture fluid, measure cell survival rate with MTS test kit (Promega company).
Method of testing is: cell serum-free medium is washed one time, adds the MTS chromophoric solution (add 2ml solution 1 and 100 μ l solution 2 in 10ml serum-free medium, fully mix) prepared in advance according to the amount of 100 μ l/well.Do not have the hole of cell to be set to this bottom outlet by one, the bias light in order to calibration solution absorbs.Cell is put into cell culture incubator and continues cultivation 2 ~ 4 hours, then absorbance value (reference wavelength 630-700nm is read by microplate reader, measure wavelength 490nm), calculate cell survival rate, to measure the numerical value of hole absorbance value/control wells absorbance value as cell survival rate.According to cell survival rate, calculate acetyl group Australia euparin to the IC50 value of Sk-hep1 cell.
IC50 refers to the concentration of a suppressed half inhibitor.Here the concentration that SK-HEP1 cell quantity is a half acetyl group Australia euparin of contrast is.The calculating of IC50 generally needs the dosage effect of mensuration more than 5, then obtains function calculating by curve fitting and obtain.
Result: acetyl group Australia euparin is to IC50 value about 16.11 μm of ol/L of Sk-hep1 cell.
Use the same method test, and the result acetyl group Australia IC50 value of euparin to other Partial tumors cells is as follows:
The IC50 value of prostate gland cancer cell PC-3 is about 30.74 μMs (micro-rub),
Be about 2.71 μMs to the IC50 value of HGC cell,
Be about 30.40 μMs to the IC50 value of PANC-1,
To the IC50 value about 29.71 μMs of hep-G2 cell.
Embodiment 2 acetyl group Australia euparin is to the growth inhibited effect of human leukemia cell
Cck-8 test kit (Japanese colleague's chemistry institute) is utilized to detect.
Step:
1) planted in 96 orifice plates uniformly by K562 cell (purchased from Chinese Academy of Sciences's cell bank), every porocyte number is 10
4individual.
2) treat adherent, dosing after spending the night, dosing (refined nurse sapogenin final concentration is respectively 50,16.67,5.56,1.85,0.62 μMs), each concentration has 3 multiple holes.
3) cultivate 48 hours, complete medium is replaced to the mixture (10:1) of serum-free medium and CCK8, hatch 2 hours in 37 DEG C of incubators.
4) be measure wavelength with 450nm, take 650nm as contrast wavelength, in microplate reader, measure reading.
Result: acetyl group Australia euparin is to the IC50 value about 2.62 μMs of K562 cell.
Claims (10)
1. the acetyl group Australia euparin of formula I is preparing the application in antitumor drug;
2. apply as claimed in claim 1, it is characterized in that, described antitumor drug is antiprostate cancer.
3. apply as claimed in claim 1, it is characterized in that, described antitumor drug is anti-gastric cancer medicament.
4. apply as claimed in claim 1, it is characterized in that, this antitumor drug is injection or tablet.
5. the method suppressing tumor cell in vitro to be bred, is characterized in that, is added in the culture fluid of tumor cell by acetyl group Australia euparin.
6. method as claimed in claim 5, it is characterized in that, described tumor cell is hepatoma carcinoma cell, stomach cancer cell, prostate gland cancer cell or pancreatic cell.
7. method as claimed in claim 6, it is characterized in that, described tumor cell is PC-3, HGC, HepG2 or PANC-1.
8. method as claimed in claim 5, it is characterized in that, the final concentration adding acetyl group Australia euparin is 1-100 μM.
9. an antitumor drug, is characterized in that, the active component of described antitumor drug is acetyl group Australia euparin.
10. antitumor drug as claimed in claim 9, it is characterized in that, described tumor is hepatoma carcinoma cell, stomach cancer cell, pancreatic cancer cell or prostate gland cancer cell.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410084099.8A CN104887657A (en) | 2014-03-07 | 2014-03-07 | Application of acetyl austroinulin in preparation of antitumor drugs |
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|---|---|---|---|
| CN201410084099.8A CN104887657A (en) | 2014-03-07 | 2014-03-07 | Application of acetyl austroinulin in preparation of antitumor drugs |
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| CN201410084099.8A Pending CN104887657A (en) | 2014-03-07 | 2014-03-07 | Application of acetyl austroinulin in preparation of antitumor drugs |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318484A (en) * | 2011-08-04 | 2012-01-18 | 阿拉尔新糖高科技有限公司 | Mechanical field sowing method for stevia rebaudiana |
| CN102813861A (en) * | 2011-06-07 | 2012-12-12 | 刘河 | Novel cough-relieving medicine prescription |
-
2014
- 2014-03-07 CN CN201410084099.8A patent/CN104887657A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102813861A (en) * | 2011-06-07 | 2012-12-12 | 刘河 | Novel cough-relieving medicine prescription |
| CN102318484A (en) * | 2011-08-04 | 2012-01-18 | 阿拉尔新糖高科技有限公司 | Mechanical field sowing method for stevia rebaudiana |
Non-Patent Citations (1)
| Title |
|---|
| ABRAHAM GARCIA,ET AL.: "Absolute configuration assignments by experimental and theoretical approaches of ent-labdane- and cis-ent-cleodane-type diterpenes isolated from Croton glabellus", 《CAN.J.CHEM.》 * |
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Application publication date: 20150909 |
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