CN104880391B - For detecting the device of particulate matter in saliva - Google Patents
For detecting the device of particulate matter in saliva Download PDFInfo
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- CN104880391B CN104880391B CN201510350946.5A CN201510350946A CN104880391B CN 104880391 B CN104880391 B CN 104880391B CN 201510350946 A CN201510350946 A CN 201510350946A CN 104880391 B CN104880391 B CN 104880391B
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Abstract
This application discloses a kind of device for being used to detect particulate matter in saliva.The detection means includes：Elongate housing, it limits the fluid passage with inlet, and wherein saliva can be flowed into the fluid passage by the inlet；Liquid stream driver, it is couple to the fluid passage, for driving flowing of the saliva in the fluid passage；Light source, it is arranged in the elongate housing, for irradiating the saliva in the fluid passage, to cause the detected particulate matter in saliva to produce corresponding optic response；Photodetector, it, which is used to detect, is detected optic response caused by particulate matter in saliva；And controller, it is used to determine the detected particulate matter in saliva according to the optic response detected.
Description
Technical field
The application is related to detection technique field, more particularly, to a kind of device for being used to detect particulate matter in saliva.
Background technology
The generation of many diseases is all relevant with the bacterium in oral cavity, such as carious tooth, periodontosis, canker sore etc..In recent years
Correlative study finds, some bacteriums in oral cavity, such as grignard streptococcus, it is also possible to causes angiocardiopathy, so to oral cavity
The daily monitoring of bacterial number, for keeping health to have positive reference significance.
The more existing device for being used to detect bacterium in saliva, such as flow cytometer.But the operation of these devices is excessively
Complexity, it is inconvenient to carry, it is impossible to meets the routine use demand of ordinary people.
The content of the invention
The purpose of the application is being easy to carry and is used to detect particulate matter in saliva easily to operate in providing one kind
The device of matter.
In the one side of the application, there is provided a kind of device for being used to detect particulate matter in saliva.The detection means
Including：Elongate housing, it limits the fluid passage with inlet, and wherein saliva can be flowed into described by the inlet
In fluid passage；Liquid stream driver, it is couple to the fluid passage, for driving stream of the saliva in the fluid passage
It is dynamic；Light source, it is arranged in the elongate housing, for irradiating the saliva in the fluid passage, to cause in saliva
It is detected particulate matter and produces corresponding optic response；Photodetector, it, which is used to detect in saliva, is detected particulate matter generation
Optic response；And controller, it is used to determine the detected particulate matter in saliva according to the optic response detected.
In certain embodiments, the optic response includes scattering light.
In certain embodiments, the light source and the photodetector both sides that to be located at the fluid passage relative.
In certain embodiments, the fluid passage is microcapillary.
In certain embodiments, the liquid stream driver is configured as driving the saliva at a predetermined velocity in the fluid
Flowed in passage.
In certain embodiments, the liquid stream driver is located at the one end of the fluid passage away from the inlet.
In certain embodiments, the inlet is located on or near one end of the elongate housing, so that the proper length
When shape housing is stretched into oral cavity, the inlet can contact saliva.
In certain embodiments, the controller is further used for counting identified detected particulate matter.
In certain embodiments, in addition to impedance detector, it is special to be used to detect the impedance of saliva in the fluid passage for it
Property；Wherein, the controller is detected according further to the impedance operator for the saliva detected come the difference determined in saliva
Particulate matter.
In certain embodiments, the light source is LASER Light Source or LED source.
In certain embodiments, the light source sends the laser of predetermined wavelength range, wherein the predetermined wavelength range bag
550 are included to 650nm.
In certain embodiments, lens are provided between the light source and circulation duct, it, which is used to assemble, comes from the light
The light beam in source, to cause the diameter of the light beam to be less than predetermined value.
In certain embodiments, in addition to the display that is arranged in the elongate housing, it is used to show the control
Determination result of the device to detected particulate matter.
In certain embodiments, the optic response of the photodetector detection includes Raman scattering light；The controller
Obtained raman scattering spectrum will be detected to be compared to identify detected particulate matter with referring to raman scattering spectrum.
In certain embodiments, the detected particulate matter is bacterium.
In certain embodiments, the controller is used to perform fluorescence removal algorithm to detect obtained raman scattering spectrum
In fluorescence interference.
In the one side of the application, a kind of toothbrush is additionally provided, it includes being used to detect according to foregoing aspect
The device of particulate matter in saliva, wherein the elongate housing is the brush holder of the toothbrush, and the inlet of the fluid passage
Positioned at the brush holder close to one end of toothbrush head.
It is the general introduction of the application above, the situation that may have simplified, summary and omissions of detail, therefore those skilled in the art
Member is it should be appreciated that the part is only Illustrative, and is not intended to restriction the application scope in any way.This general introduction portion
Divide the key feature or essential feature for being both not intended to determine claimed subject, nor be intended as claimed to determine
The supplementary means of the scope of theme.
Brief description of the drawings
Combined by following description and appended claims and with accompanying drawing, it will be more fully clearly understood that this
Apply for the above and other feature of content.It is appreciated that these accompanying drawings depict only some embodiments of teachings herein, because
This is not considered as the restriction to teachings herein scope.By using accompanying drawing, teachings herein will obtain definitely and
Explain.
Fig. 1 shows the front view of the detection means according to the application one embodiment；
Fig. 2 shows the side schematic view of the fluid passage at light source position in Fig. 1；
Fig. 3 shows the cross-sectional view of the fluid passage at light source position in Fig. 1；
Fig. 4 shows light source and another configuration of photodetector and arrangement mode；
Fig. 5 shows the schematic diagram of controller；
Fig. 6 is the raman scattering spectrum for showing some exemplary bacteriums.
Embodiment
In the following detailed description, with reference to form part thereof of accompanying drawing.In the accompanying drawings, the usual table of similar symbol
Show similar part, unless otherwise indicated by context.It is described in detail, the illustrative reality described in drawings and claims
The mode of applying is not intended to limit.In the case of without departing from the spirit or scope of the theme of the application, other implementations can be used
Mode, and other changes can be made.It is appreciated that can in the application general description, diagram is said in the accompanying drawings
The various aspects of bright teachings herein carry out a variety of differently composed configurations, replacement, combination, design, and all these all bright
Really form a part for teachings herein.
Fig. 1 shows the device 100 of particulate matter in the detection saliva according to the application one embodiment.In practical application
In, the detection means 100 can be used for the quantity for measuring some particulate matters in saliva, such as bacterium, cell or food
The quantity of residue.In some cases, the detection means 100 can also to different types of bacterium in saliva, cell or other
Particulate matter is identified, and has the material of harm to determine whether there is malignant bacteria or other.Further, the detection means
100 can also be to different types of bacterium, cell or other particulate matters are identified and differential counting.
As shown in figure 1, the detection means 100 includes photodetector 101, light source 106, liquid stream driver 108, elongate housing
111 and controller 105.
Elongate housing 111 has near-end 112 and distal end 113, and in this application, " near-end " refers to close to measured oral cavity
One end, and " distal end " refers to one end away from oral cavity.When measuring operation, compared to distally 113, elongate housing 111
Near-end 112 is closer to oral cavity, and specifically, the near-end 112 of elongate housing 111 would generally be placed in oral cavity.In some implementations
In mode, the shape of elongate housing 111 substantially can be cylindricality, consequently facilitating gripping.In some embodiments, elongate housing
111 outer wall can have depression, groove or projection.For example, elongate housing 111 can be provided with its external wall is easy to finger
The screw thread or depression of gripping.In other embodiments, the outside of elongate housing 111 can have the elastic materials such as rubber
Expect that the set that forms or circle to increase friction, stably grip elongate housing 111 and avoid sliding so as to be advantageous to operator.
Inlet 131 and liquid outlet 133 are provided with the wall of elongate housing 111.Fluid passage 103 is i.e. in liquid outlet 133
Extend between inlet 131, and positioned at the inside of elongate housing 111.In certain embodiments, for the ease of operation, enter
Liquid mouth 131 is disposed proximate to the near-end 112 of elongate housing 111, and liquid outlet 133 is disposed proximate to elongate housing 111
Distally 113.When actually measuring, testing liquid (such as saliva) enters fluid passage 103 and finally from going out by inlet 131
Liquid mouth 133 flows out.In certain embodiments, fluid passage 103 is constructed with the tubular structure of same cross-sectional.This
Sample, coordinate the driving of liquid stream driver 108, testing liquid can evenly flow in fluid passage 103, and this is advantageous to quantitative
Quantity, content or the concentration of the tested particulate matter of ground analysis.In certain embodiments, fluid passage 103 is cylindrical knot
Structure, so as to realize center of the particulate matters such as the bacterium in testing liquid, cell and swill along fluid passage 103
Line at the uniform velocity flows.This is favorably improved the degree of accuracy of detection.In certain embodiments, fluid passage 103 at it close to inlet
131 front portion has the region (not shown) of larger cross-section, and it can be used for caching testing liquid.
In some embodiments, at least part region of the side wall of fluid passage 103 is made up of high light transmittance material, such as
It is made up of optical glass, quartz or macromolecule polymer material.When testing liquid flows in fluid passage 103, light source
106 can pass through the transmission region of side wall and be irradiated to testing liquid.In addition, optic response of the testing liquid to source light
(such as scattering light) can pass through the transmission region of the side wall of fluid passage 103, be irradiated on photodetector 101.In some implementations
In example, fluid passage 103 can also have larger caliber, such as several millimeters.Cell or other suspended particulates in fluid
Material can simultaneously pass through a section of fluid passage 103 with multiple.In certain embodiments, fluid passage 103 is microtriche
Tubule, its caliber can be less than 1 millimeter, for example, 100 microns to 1 millimeter.When testing liquid is led to by the fluid compared with pipe with small pipe diameter
During road 103, cell, bacterium, swill can be one by one sequentially along the center line uniform motion of fluid passage 103, simultaneously
Microcapillary can also form negative pressuren zone in the cross section that inlet 131 contacts with testing liquid so that testing liquid can pass through
Inlet 131 enter fluid passage 103, and with the collective effect of liquid stream driver 108 so that testing liquid is in fluid passage
At the uniform velocity flowed in 103.Liquid stream driver 108 provides for the liquid stream in microcapillary stablizes accurate loading stream and loading body
Product, the single bacteria stream formed in the synergy of microcapillary and liquid stream driver 108, cell stream more accurately with stably.
In some instances, filter (not shown) can be set at the inlet 131 of fluid passage 103, such as
Filter screen.The filter can avoid in saliva some there is the particulate matter of greater particle size to enter in fluid passage 103, and
And block the fluid passage 103.
As shown in figure 1, liquid stream driver 108 is located at the distal end 113 of elongate housing 111, it is fluidly coupled to fluid
In passage 103, and close to liquid outlet 133.The liquid stream driver 108 can drive saliva at the uniform velocity to be flowed in fluid passage 103
It is dynamic.In certain embodiments, liquid stream driver 108 is micropump.Conventional micropump includes piston-style pump, barrier film declines
Type pump, gear type micropump, electrohydraulic dynamic micropump, Magnetic driving micropump, miniature electrochemistry actuating mercury air pump, constant current gravity pump
Deng.In one embodiment, liquid stream driver 108 is piezoelectric ceramics Miniature diaphragm pump, by applying driving voltage, piezoelectric element
Drive vibrating tube to do fan-shaped yaw motion in face in its resonant frequency and produce centrifugal force, centrifugal force pulls drive liquid to vibrating ball
Shell flows.Due to the continuous and Incoercibility of liquid, elastic film produces convex deformation, at the same be connected to covered on pump chamber every
Film produces convex deformation, so as to form negative pressure in fluid passage 103.In the presence of negative pressure, testing liquid is by inlet 131
It is flowed into fluid passage 103, and pump chamber is flowed into by check valve.Afterwards, electric energy stops doing power or done negative work, piezoelectric element
Stop vibration, vibrating tube sets back, and driving liquid produces backflow in the presence of the elastic-restoring force of elastic film, and barrier film is extensive
Shape is restored, will treat that drawing liquid body is extruded by liquid outlet 133.It should be noted that although piezoelectric ceramic diaphragm pump is selected in the present invention
Describe as an example the present invention liquid stream driver 108, but the present invention liquid stream driver 108 be not limited to piezoelectric ceramics every
Membrane pump, other types of micropump can also be applied.
Light source 106 is arranged at the inside of elongate housing 111, positioned at the side of fluid passage 103.Light source 106 generally may be used
To be placed on closer to the position of the near-end 112 of elongate housing 111, namely close to the position of inlet 131.By inlet
After the light irradiation that liquid in 131 incoming fluid passages 103 projects through light source 106, further through the flowing out stream of liquid outlet 133
Body passage 103.The center line for the light beam that light source 106 is sent is by fluid passage 103, so as to be irradiated to the prepare liquid wherein flowed
On body.In certain embodiments, the beam center line of light source 106 can be with the vertical and intersectant centerline of fluid passage 103.One
In a little embodiments, light source 106 is LASER Light Source, such as the light source formed by laser diode.In a specific exemplary reality
Apply in example, light source 106 is all solid state laser (DPL), and it has small volume, in light weight, efficiency high, stable performance, reliability
Well, the advantages that long lifespan, high beam quality.Because the thermic load of its unit output reduces, higher power can be obtained, it is longer
Lifetime of system and reliability.In certain embodiments, light source 106 can also be light emitting diode (LED) light source.
Fig. 2 is shown in Fig. 1 in the side schematic view of the opening position fluid passage 103 of light source 106.As shown in Fig. 2 light source
Lens 161 are also provided between 106 and fluid passage 103, the light beam that the lens 161 are launched to convergent light source 106,
So that gained irradiation treats the Light beam spot diameter change of fluid measured, such as convergent beam make it that it is default that its spot diameter is less than
Value.In some instances, lens 161 may be constructed such that single element；In other examples, lens 161 can also be by
It is integrated on the outer wall of fluid passage 103, such as lens 161 is set in the region that fluid passage 103 is irradiated by light source 106.
This mode is easily achieved, because fluid passage 103 can use the printing opacity material such as quartz, glass, high polymer material
Material is formed.In some specific embodiments, the spot diameter that the outgoing beam of light source 106 is formed on fluid measured is treated can be with treating
The diameter of the bacterium that may be included in fluid measured or cell (such as bacterium to be detected or cell) is close.In general, coccus
About l μm of diameter, medium sized bacillus grows 2~3 μm, wide 0.3~0.5 μm.Correspondingly, in certain embodiments, light source 106
Lens 161 between fluid passage 103 are arranged to can be so that the spot diameter that the light beam that light source 106 is launched is formed be
0.1 to 10 μm, or preferably, 0.3 to 3 μm.
Still as shown in figure 1, photodetector 101 is arranged at the inside of elongate housing 111, positioned at fluid passage 103 and light
The relative other side in the position of source 106.In certain embodiments, photodetector 101 is photoelectric sensor, its by with
In the optic response for treating fluid measured and launching light source 106 light beam is converted into electric signal.Photoelectric sensor includes but is not limited to light
Electric diode, charge coupling device, photomultiplier etc..Photodiode has less device size, therefore can use
Photodiode array detector detects optical signal.It is for instance possible to use avalanche photodide detects optical signal.Photodiode
Array can be arranged with least part periphery of the outer wall of hydrodynamic form passage 103.In addition it is also possible to using photomultiplier come detection light
Signal.The response time of photomultiplier is short, only nanosecond；Spectral response characteristic is good, in 200~900nm spectrum
Area, quantum yield of luminscence are all higher.The gain continuously adjustable of photomultiplier, therefore dim light is measured highly beneficial.Photoelectricity
It is important to note that stability problem, operating voltage is sufficiently stable when multiplier tube is run, operating current and power can not be too big.Light
The power consumption of electric multiplier tube is generally below 0.5W；Maximum anode current is in several milliamperes.It should be noted that photomultiplier is entered
Row dark adaptation is handled, and pays attention to good magnetic screen.It is still weaker, it is necessary to by linear from the electric signal of photomultiplier output
Controller can be just supplied to carry out particulate matter analysis and identification after amplifier or logafier amplification.
In certain embodiments, the optic response that photodetector 101 predominantly detects is scattering light.According to angle after scattering
Difference, the scattering light are divided into forward angle light scatter (forward scatter, FSC) and side scatter (side
Scatter, SSC), both the above signal is from original light beam of the transmitting of light source 106, thus its wavelength and light source 106
Wavelength is identical.Technology of preparing of the light independent of any cell sample is scattered, therefore is referred to as the physical parameter of cell or intrinsic
Parameter.Scatter light intensity and its spatial distribution it is related to the intrinsic parameter of tested particle, such as with the size of tested bacterium, shape
State, plasma membrane are related to internal structure.The bacterium not suffered damage has the scattering of characteristic to light, therefore can utilize difference
Scattered light signal variety classes bacterium made a distinction and counted.In addition, Stomatocyte and swill for coming off etc.
Scattering of the grain material to light differs markedly from bacterium, thus can be distinguished itself and bacterium.Specifically, forward angle light scatter with
The size of tested cell is relevant, and exactly, it is closely related with the square value of cell dia.Correspondingly, it is specific real at some
Apply in example, photodetector 101 is located at the center face position of light source 106, the center line of the outgoing beam of light source 106 and fluid passage
103 vertical and intersectant centerline simultaneously overlaps with the center line of photodetector inspection, so that the light that photodetector 101 detects
It is mainly forward angle light scatter light to learn response.
In further embodiments, light source 106 can be monochromatic excitation light source, such as the LASER Light Source of Single wavelength, such as
Ultraviolet light, visible ray or near infrared light.In certain embodiments, the wave-length coverage that LASER Light Source can use for 200 to
1100nm.In some instances, the wavelength of LASER Light Source is 244 to 364nm Ultra-Violet Laser, and it is adapted to measure biomolecule,
And it can effectively suppress fluorescent.In some instances, the wavelength of LASER Light Source is 660 to 830nm infrared and near red
Outer laser, it can suppress fluorescent.Can have of a relatively high power using the light source 106 of LASER Light Source, when from
When the light of light source 106 is irradiated on the testing liquid flowed in fluid passage 103, light is by the material (example in testing liquid
Such as cell, food molecule or other material molecules) scatter and occurrence frequency change.Correspondingly, photodetector 101 can be detected and treated
The Raman scattering light of liquid-scattering is surveyed, and generates raman scattering spectrum.In some instances, semiconductor optoelectronic can be used
Sensor (such as InGaAs array detectors) or CCD photoelectric detectors detect Raman scattering light.In detection Raman scattering
During light, one-time detection can cover the light in wider frequency range, such as the light of 50 to 4000 wave numbers of covering simultaneously, from
And organic matter and inorganic matter can be detected and analyzed simultaneously.In addition, the detection of Raman scattering light is particularly suitable for inspection
The aqueous solution is surveyed, and the testing liquid from oral cavity is generally all the aqueous solution.In actual applications, it can also be combined, analyze resonance
Raman, surface-enhanced Raman and non-linear Raman spectroscopy come improve material detection accuracy.
Fig. 3 is shown in Fig. 1 in the cross-sectional view of the opening position fluid passage 103 of light source 106.As shown in figure 3, light source
106 and photodetector 101 be located directly opposite in the both sides of fluid passage 103, wherein photodetector 101, which is used to detecting forward angle, dissipates
Penetrate light.In the embodiment shown in fig. 3, detection means also includes multiple photodetector 101A, and it is used to detect side scatter
Light.In certain embodiments, photodetector 101A can with the circumferentially distributed of the place section of light source 106 of hydrodynamic form passage 103, and
And the part or all of region between light source 106 and photodetector 101 is occupied, so that the light for scattering to different directions is equal
It can be detected.Wherein, each photodetector 101A relative to the angle of light source 106 is predetermined, and each light
The signal that detector 101A detects to obtain can be distinguished by controller 105.
Fig. 4 shows light source and another configuration of photodetector and arrangement mode.As shown in figure 4, in fluid passage 103
One section is provided with two pairs of light source-light detectors, i.e. light source 106B and photodetector 101B, and light source 106C and light detection
Device 101C.Forward angle light scatter light of the wherein photodetector 101B for detecting the light that light source 106B is sent, and photodetector
101C is used to detect forward angle light scatter light corresponding to the light that light source 106C is sent.So, same candidate particles material can be by
Irradiate in different directions, and more scattering optical informations can be obtained.In some instances, photodetector 101B can also be used
In the side scatter light for the light that detection light source 106C is sent, and photodetector 101C can be also used for detecting light source 106B hairs
The side scatter light of the light gone out.In certain embodiments, light source 106B, 106C and photodetector 101B and 101C it
Between the side wall of fluid passage 103 on, one or more photodetector 101D can also be set, for detecting the side of other angles
Light is scattered to angle.
Still as shown in figure 1, light source 106 launches such as wavelength as 550 to 650nm continuously adjustabes laser, it is by saturating
Mirror 161 is converged to a diameter of 1 μm or so of laser beam.The center line of the laser beam and the center line of fluid passage 103 hang down
It is straight to overlap.When laser beam is irradiated to sequentially at the uniform velocity by the testing liquid of fluid passage 103, light produces scattering effect.
After the scattering of the particulate matters such as mouth desquamated cells, oral bacteria and swill, forward angle light scatter light and/or lateral angle
Scattering illumination is mapped on photodetector 101.The optical signal is converted into electric signal by photodetector 101.The electric signal can provide
Handled to controller 105 and by it.
In certain embodiments, controller 105 is located in elongate housing 111, and the optics detected by photodetector 101 rings
After answering (scattering light) to be converted into electric signal, received by controller 105.Controller 105 is determined by the analysis to electric signal
It is detected the type and quantity of particulate matter in saliva.In certain embodiments, it is thin can there be some in controller 105 in advance
The forward angle light scatter light or side scatter light curve or figure of bacterium, cell or particulate matter, these curves prestored or
Figure can be used to compared with detected curve or figure, so as to identify different cell or particulate matter, or
Further different cell or particulate matter are counted, to determine the content in testing liquid.
As shown in figure 5, controller includes signal amplifier 151, analog-digital converter 152 and microprocessor 153.Photodetector
101 change according to different optic responses, and change in electric corresponding to generation, the electric signal is put by signal amplifier 151
After big, data signal is converted in input analog-to-digital converter 152, final data signal, which is transfused in microprocessor 153, is divided
Analysis is handled.For example, microprocessor 153 can be by data signal compared with the scattering curve or figure that prestore.
In certain embodiments, light source 106 and photodetector 101 can be used for carrying out raman scattering spectrum detection.At this
Kind in the case of, controller can call prestore reference raman scattering spectrum (such as provide a flash memory with store with reference to drawing
Graceful scattering spectrum), such as refer to raman scattering spectrum corresponding to different bacterium.Collecting the Raman diffused light of testing liquid
After spectrum, controller can belong to according to the characteristic peak of the raman scattering spectrum of collection carries out first ownership identification to spectrogram, then
According to characteristic peak peak intensity than carrying out secondary ownership, so as to compare and identify the bacterial species included in testing liquid.At some
In the case of, light source 106, which irradiates material, may produce interference fluorescence.Correspondingly, controller can perform fluorescence and remove algorithm, with
The interference signal of fluorescence is removed from the raman scattering spectrum of collection, this is advantageous to the accuracy and precision that improve detection.
Fig. 6 is the raman scattering spectrum for showing some exemplary bacteriums.
As shown in fig. 6, staphylococcus aureus, proteus, Escherichia coli have the Raman signal of feature：Golden yellow Portugal
Grape coccus is 725,1330,1450cm-1Have an obvious Raman vibration peak, proteus is 650,725,950,1325,1463cm-1Have an obvious Raman vibration peak, Escherichia coli are 650,950,1125,1242,1320,1457cm-1There is obvious Raman to vibrate
Peak.The position of these bacterium Raman vibration peaks and intensity difference are obvious.Therefore, controller can be from the raman scattering spectrum of collection
Middle these Raman vibration peaks of identification, so that it is determined that corresponding bacterial species.
Still as shown in figure 1, in certain embodiments, detection means 100 can also include impedance detector 102.The impedance
Detector 102 includes impedance negative electrode 121, impedance anode 122 and impedance detection module (not shown).Impedance negative electrode 121
It is arranged on the relative both sides of fluid passage 103 with impedance anode 122, and impedance detection module and impedance negative electrode 121 and impedance
The phase of anode 122 couples.Impedance detection module is used to load voltage regulation signal between impedance negative electrode 121 and impedance anode 122, and
The voltage regulation signal caused electric current between the two electrodes is measured, so that it is determined that corresponding impedance value.When cell, bacterium, food
When the different objects such as thing residue are by detecting two interelectrode detection zones, surveyed between impedance anode 122 and impedance negative electrode 121
The impedance value obtained will change, and the change in impedance value caused by different objects is also different.Impedance detector 102 can be with
Further it is connected with controller 105, it detects obtained impedance value and can be provided in controller 105 to carry out processing point
Analysis.Controller 105 can be by the impedance of the impedance value measured and the impedance value or other particulate matters of the bacteria sample of reference
Value is contrasted, so that it is determined that being detected particulate matter in saliva, and then is counted accordingly.In certain embodiments, hinder
The testing result of anti-detector 102 can be used to be modified the result that photodetector obtains.For example, impedance detection result
Different particulate matters can be used to distinguish between, only when impedance detection result and light testing result determine tested particulate matter
For bacterium when, the ability of controller 105 counts to tested particulate matter, otherwise without counting.
Still as shown in figure 1, the detection means 100 can also include display 109, it is, for example, LCDs.This is aobvious
Show that device 109 can be embedded into elongate housing 111, and be connected to controller 105.Controller 105 analyzes and processes resulting
Testing result can be shown on display 109, so as to obtain different detected particulate matters in specific saliva immediately
Information.In certain embodiments, controller 105 can also be compared the quantity for detecting obtained harmful bacteria with predetermined threshold
Compared with, and provide alarm signal when more than predetermined threshold.Display 109 can show alarm signal, to prompt user to note
Meaning dental health.In further embodiments, controller 105 can also will detect the quantity of obtained mouth desquamated cells
Compared with predetermined threshold, and alarm signal is provided when it exceedes predetermined threshold.Display 109 can show warning
Signal, to prompt user to pay attention to oral healthy condition, for example whether canker sore be present.In certain embodiments, detection dress
Other reminding modules, such as loudspeaker can also be included by putting 100, come the information of particulate matter detected to user's prompting.
In some embodiments, the detection means 100 also includes storage device, for the detection to a period of time
Data are stored, and the particulate matter content situation of change in saliva of buccal cavity in a period of time is analyzed so as to realize.Example
Such as, it can start to gather when user starts to brush teeth and store detection data, until end of brushing teeth.Adopted during whole brush teeth
At least a portion (data at the beginning and end of such as) of particulate matter data in the saliva for collecting and storing can be shown
Out, so that user observes the effect brushed teeth.In some specific embodiments, the detection means 100 also includes data
Port, such as Micro-USB ports, so that the detection data can be transferred in external equipment and be analyzed and processed.
As shown in figure 1, the detection means 100 also includes power supply 112, the power supply 112 is integrated in elongate housing 111
In, it is powered for other electronic components to light source, display, controller, impedance detector etc..In some embodiments
In, power supply can be replaceable battery, such as lithium battery, Ni-MH battery etc., and it is installed in battery case.In other realities
Apply in mode, power supply can be the power supply without changing, such as rechargable power supplies (such as rechargeable lithium battary).The detection fills
One or more charging modes can be provided to be charged to rechargable power supplies by putting 100.In some embodiments, the detection
The charging port for connecting external power source, such as Micro-USB ports can be provided with device 100.The charging port is also
It is connected to rechargable power supplies.By the charging port, external power source can be by electrical energy transportation to rechargable power supplies, and it is entered
Row charging, additionally can directly power to the detection means.
In the embodiment shown in fig. 1, detection means 100 is integrated on toothbrush.For example, the brush holder of toothbrush defines stream
Body passage, inlet 131 can be arranged on toothbrush head 110.So, user can carry out mouth while daily brush teeth
Chamber Bacteria Detection, meet the convenience of routine testing.Preferably, user can detect once before brushing teeth, and brush teeth
Detect once again afterwards, so that it can compare this testing result difference twice, and determine how is the effect brushed teeth.At some
In embodiment, head toothbrushes detachably combine with brush holder, so that head toothbrushes are changed in timing after a certain time.Accordingly
Ground, the part that fluid passage is located at head toothbrushes can be connected with each other with positioned at the part of brush holder by screw thread.It is appreciated that
In some other embodiments, detection means 100 can also be integrated on other products, such as tooth flusher etc..
In this application, word " comprising " is not excluded for other elements and step, and wording " one ", "one" is not excluded for
Plural number.In the practical application of invention, the function of cited multiple technical characteristics in the possible perform claim requirement of a part.
Any reference in claim should not be construed as restriction on its scope.
Various embodiments of the present invention are exemplarily illustrated above in association with accompanying drawing.Those skilled in the art are according to this specification
Disclosure is readily apparent that, each embodiment suitably can be adjusted and reconfigured according to being actually needed,
Without departing from spirit herein.The protection domain of the application is defined by following claims.
Claims (16)
1. a kind of toothbrush for being used to detect particulate matter in saliva, including：
Elongate housing, it limits the fluid passage with inlet, and wherein saliva can be flowed into described by the inlet
In fluid passage；
Liquid stream driver, it is couple to the fluid passage, for driving flowing of the saliva in the fluid passage；
Light source, it is arranged in the elongate housing, for irradiating the saliva in the fluid passage, to cause in saliva
It is detected particulate matter and produces corresponding optic response；
Photodetector, it, which is used to detect, is detected optic response caused by particulate matter in saliva；And
Controller, it is used to determine the detected particulate matter in saliva according to the optic response detected
The fluid passage also has liquid outlet, and the liquid being flowed into by inlet in the fluid passage irradiates by light source
Afterwards, the fluid passage is flowed out by the liquid outlet, the liquid outlet is arranged on the wall of the elongate housing；
Wherein described elongate housing is the brush holder of the toothbrush, and the inlet of the fluid passage is located at the brush holder close to tooth
One end of brush brush.
2. toothbrush according to claim 1, it is characterised in that the optic response includes scattering light.
3. toothbrush according to claim 1, it is characterised in that the light source is located at the fluid with the photodetector and led to
The relative both sides in road.
4. toothbrush according to claim 1, it is characterised in that the fluid passage is microcapillary.
5. toothbrush according to claim 1, it is characterised in that the liquid stream driver be configured as driving the saliva with
Predetermined speed flows in the fluid passage.
6. toothbrush according to claim 1, it is characterised in that the liquid stream driver is located at the fluid passage away from institute
State one end of inlet.
7. toothbrush according to claim 1, it is characterised in that the inlet is located on or near the one of the elongate housing
End, during so that the proper elongate housing is stretched into oral cavity, the inlet can contact saliva.
8. toothbrush according to claim 1, it is characterised in that the controller is further used for being detected to identified
Particulate matter is counted.
9. toothbrush according to claim 1, it is characterised in that also including impedance detector, it is used to detect the fluid
The impedance operator of saliva in passage；Wherein, the controller determines according further to the impedance operator for the saliva detected
Difference in saliva is detected particulate matter.
10. toothbrush according to claim 1, it is characterised in that the light source is LASER Light Source or Light-Emitting Diode light
Source.
11. toothbrush according to claim 10, it is characterised in that the light source sends the laser of predetermined wavelength range, its
Described in predetermined wavelength range include 550 to 650nm.
12. toothbrush according to claim 1, it is characterised in that lens are provided between the light source and circulation duct, its
For assembling the light beam from the light source, to cause the diameter of the light beam to be less than predetermined value.
13. toothbrush according to claim 1, it is characterised in that also include the display being arranged in the elongate housing,
It is used to show determination result of the controller to being detected particulate matter.
14. toothbrush according to claim 1, it is characterised in that the optic response of the photodetector detection includes Raman
Scattering light；The controller is compared obtained raman scattering spectrum is detected to identify quilt with referring to raman scattering spectrum
Detect particulate matter.
15. toothbrush according to claim 14, it is characterised in that the detected particulate matter is bacterium.
16. toothbrush according to claim 14, it is characterised in that the controller is used to perform fluorescence removal algorithm to examine
Fluorescence interference in the raman scattering spectrum measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510350946.5A CN104880391B (en) | 2015-06-23 | 2015-06-23 | For detecting the device of particulate matter in saliva |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510350946.5A CN104880391B (en) | 2015-06-23 | 2015-06-23 | For detecting the device of particulate matter in saliva |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104880391A CN104880391A (en) | 2015-09-02 |
| CN104880391B true CN104880391B (en) | 2018-03-06 |
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ID=53947968
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510350946.5A Active CN104880391B (en) | 2015-06-23 | 2015-06-23 | For detecting the device of particulate matter in saliva |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655447B (en) * | 2019-01-28 | 2022-04-08 | 广东海天创新技术有限公司 | Detection system and method for microbial enumeration |
| CN116793907B (en) * | 2022-03-16 | 2024-05-14 | 上海勘测设计研究院有限公司 | Multidirectional diffraction scattering type particle size analyzer and particle detection method |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7978329B2 (en) * | 2000-08-02 | 2011-07-12 | Honeywell International Inc. | Portable scattering and fluorescence cytometer |
| US7277166B2 (en) * | 2000-08-02 | 2007-10-02 | Honeywell International Inc. | Cytometer analysis cartridge optical configuration |
| JP2002181812A (en) * | 2000-12-15 | 2002-06-26 | Matsushita Electric Ind Co Ltd | Toothbrush type biosensor |
| WO2009088408A1 (en) * | 2008-01-07 | 2009-07-16 | Dynamic Throughput Inc. | Discovery tool with integrated microfluidic biomarker optical detection array device and methods for use |
| US8202230B2 (en) * | 2009-08-20 | 2012-06-19 | Colgate-Palmolive Company | Oral care implement having a liquid collection system |
| CN104705996A (en) * | 2013-12-12 | 2015-06-17 | 鸿富锦精密工业(武汉)有限公司 | Smart toothbrush |
| CN103822868A (en) * | 2014-03-14 | 2014-05-28 | 大连海事大学 | Device and method for detecting grain size of phytoplankton in seawater |
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