CN104865341B - Thin-layer chromatography detection method of traditional Chinese medicine composition for increasing animal immunity - Google Patents
Thin-layer chromatography detection method of traditional Chinese medicine composition for increasing animal immunity Download PDFInfo
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Abstract
The invention discloses a thin-layer chromatography detection method of a traditional Chinese medicine composition. The thin-layer chromatography detection method comprises the following steps: a, preparing a test solution; b, preparing a reference solution; c, respectively applying the test solution, the reference solution of astragaloside, the reference solution of oleanolic acid and the reference solution of pinoresinol diglucoside to the same silica gel plate, developing by taking the lower solution of chloroform-ethyl acetate-methanol-water as a developing agent, taking out, airing, and heating by taking 2% vanillic aldehyde sulfuric acid solution as a colour developing agent till the colour of spots is developed clearly; and d, viewing the silica gel plate after the colour is developed. The thin-layer chromatography detection method of the traditional Chinese medicine composition disclosed by the invention can be used for simultaneously detecting astragalus membranaceus, glossy privet fruits and the bark of eucommia in the traditional Chinese medicine composition; the detection result is accurate and reliable; and simultaneously, the thin-layer chromatography detection method disclosed by the invention has the advantages of being few in process, simple and convenient to operate, short in detection time, low in cost and the like and is particularly applied to industrial application.
Description
Technical field
The present invention relates to a kind of thin-layered chromatography detection method of the Chinese medicine composition improving animal resistance.
Background technology
Thin layer chromatography (method) is a kind of conventional Chinese medical herb method, has that equipment is simple, easy and simple to handle, separating rate
Hurry up, sensitivity and resolution higher the advantages of.
The Radix Astragali, also known as Radix Astragali, are the dry root of leguminous plant Radix Astagali, have invigorating QI to consolidate the body surface resistance, profit water detumescence, poison holding row
The effects such as pus, granulation promoting, be a kind of Chinese crude drug.The complex chemical composition of the Radix Astragali, mainly has saponinss, flavonoid and its glycoside, many
Saccharide, amino acidses etc..Fructus Ligustri Lucidi is the dry mature fruit of Oleaceae Fructus Ligustri Lucidi platymiscium Fructus Ligustri Lucidi, and sweet in the mouth, slight bitter are puckery, have taste
Invigorating the liver and kidney, the work(of improving eyesight black hair, can be used for the hepatic and renal YIN deficiency, vertigo and tinnitus, early whitening of beard and hair, poor vision, Heat Diabetes, hectic fever due to YIN-deficiency tide
The symptoms such as heat, are a kind of Chinese crude drugs.The complex chemical composition of Fructus Ligustri Lucidi, mainly has three mushrooms, flavonoid, phenylethanol etc..Du
Secondary be Angiospermae Eucommiaceae Cortex Eucommiae platymiscium bark, be a kind of Chinese crude drug.
In prior art, the medical material such as the Radix Astragali, Fructus Ligustri Lucidi, Cortex Eucommiae and combinations thereof is generally differentiated using thin layer chromatography,
However, existing discrimination method can only carry out qualitative identification to one of the Radix Astragali, Fructus Ligustri Lucidi, Cortex Eucommiae medical material, such as: Liu Xiaoqing etc.
(Hu scape Lian Lixiao gorgeous Men Qiming Kang Ting state of Liu Xiao Qing Li army. in Milkvetch Root, calycosin glucoside thin layer chromatography is quick
Differentiate. Liaoning University of TCM's journal, in July, 2013, volume 15, the 7th phase .) prepared for examination by specific pre-treatment
Product solution, and be developing solvent from specific chloroform-methanol-water (7.5:2.5:0.2), thin layer speckle could be obtained clear
The preferable testing result of clear, separating degree；Zhou Yang etc. (raise Liu Li Xu De life week. the bitter thin layer chromatography of special Fructus Ligustri Lucidi in Fructus Ligustri Lucidi decoction pieces
Differentiate. Chinese Pharmaceutical, on October 5th, 2013, volume 22, the 19th phase .) to prepare test sample by specific pre-treatment molten
Liquid, and be developing solvent from specific acetic acid ethyl ester-acetone-formic acid-water (6:3:1:1), thin layer clear spot could be obtained, divide
From the preferable testing result of degree；Liu Ling etc. (Liu Ling Bao Jia section. Eucommia ulmoides indentification by TLC is studied. China National doctor among the people
Medicine, 2013 .) need testing solution is prepared by specific pre-treatment, and from specific chloroform-n-butanol-water-
Acetic acid solution (1:7:1:1) is developing solvent, could obtain thin layer clear spot, the preferable testing result of separating degree, its developing solvent
Even screening test demonstrate the minor adjustment of each group component in developing solvent also testing result can be brought with very big shadow
Ring.
Chinese document (Lee Cao Guowen that admires less Zhai becomes monarch. and the thin layer of compound recipe gold granules differentiates. Chinese veterinarian's medical magazine,
2010, the 6th phase .) disclosing the thin-layer identification method of compound recipe gold granules, the method needs to the Radix Astragali, Fructus Ligustri Lucidi etc. respectively
Need testing solution is prepared using different pre-treatments, selects different developing solvents respectively, carry out thin layer chromatography detection respectively,
The method can not carry out pre-treatment and thin layer chromatography detection to the component such as the Radix Astragali, Fructus Ligustri Lucidi in compound granular simultaneously, there is operation
Many, many defects such as complex operation, detection time length, cost height.
Accordingly, it would be desirable to a kind of operation of invention is few, easy and simple to handle and the Radix Astragali, Fructus Ligustri Lucidi, the Cortex Eucommiae can be detected simultaneously
New method.
Content of the invention
It is an object of the invention to provide a kind of thin-layered chromatography detection method of Chinese medicine composition.
A kind of thin-layered chromatography detection method of Chinese medicine composition that the present invention provides, comprises the following steps:
A, prepare need testing solution: take Chinese medicine composition, add methanol a, supersound process, filter, take supernatant；Supernatant
With microporous filter membrane filtration, it is evaporated, residue is with, after 10%v/v ammonia solvent, with water-saturated n-butanol extraction, being extracted liquid；Will
Extract is evaporated, and residue methanol b dissolves, and obtains final product need testing solution；
B, preparation reference substance solution: take astragaloside, oleanolic acid, pinoresinol diglucoside to be dissolved with methanol respectively,
Obtain the reference substance solution of astragaloside, the reference substance solution of oleanolic acid, the reference substance solution of pinoresinol diglucoside；
C, by need testing solution, the reference substance solution of astragaloside, the reference substance solution of oleanolic acid, (+)-Pinoresinol two Fructus Vitis viniferae
The reference substance solution of glucosides, distinguishes point sample in same silica gel plate, with lower floor's solution of chlorofonn-ethylacetate-methanol-water for launching
Agent, launches, and takes out, dries, and with 2% vanillin-sulfuric acid solution as developer, 105 DEG C to be heated to spot development clear；
D, inspect the silica gel plate after colour developing, you can.
Further, in step a, described Chinese medicine composition is made up of following raw material: the Radix Astragali, Fructus Ligustri Lucidi, the Cortex Eucommiae.
Further, in step a, described Chinese medicine composition is made up of the raw material of following parts by weight: 18 parts of the Radix Astragali, female
Loyal son 9 parts, 9 parts of the Cortex Eucommiae.
Further, in step a, described Chinese medicine composition is prepared from according to following steps:
1., take the Radix Astragali, Fructus Ligustri Lucidi, the Cortex Eucommiae, be crushed to 80 mesh, dry, respectively obtain radix astragali coarse powder, Fructus Ligustri Lucidi coarse powder, Du
Secondary coarse powder；
2., under the conditions of -10 DEG C～-20 DEG C, the radix astragali coarse powder that 1. step is obtained is crushed to 100 mesh～800 further
Purpose granularity, obtains Radix Astragali superfine powder；
3., Fructus Ligustri Lucidi coarse powder is mixed with Cortex Eucommiae coarse powder, boiled twice with decocting, filter, concentrate, obtain concentrated solution；
4. the Radix Astragali superfine powder that 2. concentrated solution, 3. obtaining step and step obtain mixes, and pelletizes, dries, crosses 80 mesh
Sieve, obtains final product.
Further, in step a, Chinese medicine composition is 1:15g/ml with the w/v of methanol a；Methanol a, 10%v/
V ammonia, water-saturated n-butanol, the volume ratio of methanol b are 6:2:3:1.
Further, in step a, the time of supersound process is 60min；The aperture of microporous filter membrane is 0.45 μm.
Further, in step b, the reference substance solution of astragaloside, the reference substance solution of oleanolic acid, (+)-Pinoresinol two Portugal
The concentration of the reference substance solution of polyglycoside is respectively 1mg/ml.
Further, in step c, described silica gel plate is silica gel g lamellae.
Further, in step c, in chlorofonn-ethylacetate-methanol-water, chloroform, ethyl acetate, methanol, the volume of water
Than for 20:40:22:1.
Further, in step d, silica gel plate after colour developing, on position corresponding with reference substance chromatograph, test sample chromatograph
The speckle of display same color.
The thin-layered chromatography detection method of Chinese medicine composition of the present invention, can be to the Radix Astragali in Chinese medicine composition, Fructus Ligustri Lucidi, Du
Secondary detected simultaneously, testing result accurately, reliable, meanwhile, the inventive method has that operation is few, easy and simple to handle, detection time
Short, low cost and other advantages, are especially suitable for the application in industry.
Obviously, the above according to the present invention, according to ordinary technical knowledge and the customary means of this area, without departing from
Under the premise of the present invention above-mentioned basic fundamental thought, modification, replacement or the change of other various ways can also be made.
The specific embodiment of form by the following examples, remakes further specifically to the above of the present invention
Bright.But this scope being interpreted as the above-mentioned theme of the present invention should not be only limitted to Examples below.All based on the above of the present invention
The technology realized belongs to the scope of the present invention.
Brief description
Fig. 1 is the thin layer chromatography testing result of Chinese medicine composition of the present invention, wherein, " loose " representative " (+)-Pinoresinol glucosulfone
Glycosides ", " first " representative " astragaloside ", " neat " representative " oleanolic acid ", " sample " representative " test sample ".
Specific embodiment
Used in the specific embodiment of the invention, raw material, equipment are known product, are obtained by buying commercially available prod.
Embodiment 1, the thin-layered chromatography detection method of Chinese medicine composition of the present invention
1st, prepare Chinese medicine composition
Count by weight: 18 parts of the Radix Astragali, 9 parts of Fructus Ligustri Lucidi, 9 parts of the Cortex Eucommiae；By three kinds of medical materials respectively using Universalpulverizer
It is crushed to 80 mesh, dries, make the moisture Control of each coarse powder medical material 6%～8%；Under the conditions of temperature is -10 DEG C～-20 DEG C,
Radix astragali coarse powder is crushed to the superfine powder that granularity is 100～800 mesh granularities；By Fructus Ligustri Lucidi and the mixing of Cortex Eucommiae coarse powder, boiled with decocting
Twice, filter, mixing filtrate concentrates, finally concentrated solution and Radix Astragali superfine powder are mixed, granulation post-drying, cross 80 mesh sieves, obtain
Chinese medicine composition.
2nd, detection method
Above-mentioned traditional Chinese medicine particle is ground, precision weighs powder 2.0g, is placed in triangular flask, plus methanol 30ml, surpass
Sonication 60min, filters, takes supernatant；Supernatant is filtered with 0.45 μm of microporous filter membrane (ptfe), is placed in evaporating dish and is evaporated；
Residue 10ml 10% (v/v) ammonia solvent, places 10min, frequently shakes；Solution is moved in separatory funnel, uses 15ml water
Saturation n-butanol extraction 3 times；Combining extraction liquid, is evaporated with evaporating dish, and residue adds the dissolving of 5ml methanol, obtains final product need testing solution；
Take astragaloside, oleanolic acid, pinoresinol diglucoside, add methanol, be respectively prepared every 1ml and contain the molten of 1mg
Liquid, as reference substance solution.
According to thin layer chromatography, (2010 editions annex b) tests of Pharmacopoeia of People's Republic of China, draw above-mentioned test sample molten
The each 3 μ l of liquid, reference substance solution, put respectively on same silica gel g lamellae, with chlorofonn-ethylacetate-methanol-water (20:40:
Lower floor's solution 22:1) is developing solvent, launches, and takes out, dries, and spray, with 2% vanillin-sulfuric acid solution, is heated to speckle at 105 DEG C
Point colour developing is clear；Its testing result is shown in Fig. 1, on position corresponding with reference substance chromatograph, test sample Chromatogram display same color
Speckle.
The thin-layered chromatography detection method of Chinese medicine composition of the present invention, can be to the Radix Astragali in Chinese medicine composition, Fructus Ligustri Lucidi, Du
Secondary detected simultaneously, testing result accurately, reliable, meanwhile, the inventive method has that operation is few, easy and simple to handle, detection time
Short, low cost and other advantages, are especially suitable for the application in industry.
Claims (8)
1. a kind of thin-layered chromatography detection method of Chinese medicine composition it is characterised in that: described discrimination method comprises the following steps:
A, prepare need testing solution: take Chinese medicine composition, add methanol a, supersound process, filter, take supernatant；Supernatant is with micro-
Hole filter membrane filtration, is evaporated, and residue is with, after 10%v/v ammonia solvent, with water-saturated n-butanol extraction, being extracted liquid；To extract
Liquid is evaporated, and residue methanol b dissolves, and obtains final product need testing solution；
B, preparation reference substance solution: take astragaloside, oleanolic acid, pinoresinol diglucoside to be dissolved with methanol respectively, obtain
The reference substance solution of astragaloside, the reference substance solution of oleanolic acid, the reference substance solution of pinoresinol diglucoside；
C, by need testing solution, the reference substance solution of astragaloside, the reference substance solution of oleanolic acid, pinoresinol diglucoside
Reference substance solution, respectively point sample is in same silica gel plate, with lower floor's solution of chlorofonn-ethylacetate-methanol-water as developing solvent,
Launch, take out, dry, with 2% vanillin-sulfuric acid solution as developer, 105 DEG C are heated to spot development clearly, after being developed the color
Silica gel plate；Wherein, in chlorofonn-ethylacetate-methanol-water, chloroform, ethyl acetate, methanol, the volume ratio of water are 20:40:
22:1；
D, inspect the silica gel plate after colour developing, you can；
Described Chinese medicine composition is made up of following raw material: the Radix Astragali, Fructus Ligustri Lucidi, the Cortex Eucommiae.
2. thin-layered chromatography detection method according to claim 1 it is characterised in that: in step a, described Chinese medicine composition is
It is made up of the raw material of following parts by weight: 18 parts of the Radix Astragali, 9 parts of Fructus Ligustri Lucidi, 9 parts of the Cortex Eucommiae.
3. thin-layered chromatography detection method according to claim 1 and 2 it is characterised in that: in step a, described Chinese medicine composition
Thing is prepared from according to following steps:
1., take the Radix Astragali, Fructus Ligustri Lucidi, the Cortex Eucommiae, be crushed to 80 mesh, dry, respectively obtain radix astragali coarse powder, Fructus Ligustri Lucidi coarse powder, the Cortex Eucommiae thick
Powder；
2., under the conditions of -10 DEG C～-20 DEG C, the radix astragali coarse powder that 1. step is obtained is crushed to 100 mesh～800 purposes further
Granularity, obtains Radix Astragali superfine powder；
3., Fructus Ligustri Lucidi coarse powder is mixed with Cortex Eucommiae coarse powder, boiled twice with decocting, filter, concentrate, obtain concentrated solution；
4. the Radix Astragali superfine powder that 2. concentrated solution, 3. obtaining step and step obtain mixes, and pelletizes, dries, crosses 80 mesh sieves, that is,
?.
4. thin-layered chromatography detection method according to claim 1 it is characterised in that: in step a, Chinese medicine composition and methanol
The w/v of a is 1:15g/ml；Methanol a, 10%v/v ammonia, water-saturated n-butanol, the volume ratio of methanol b are 6:2:3:
1.
5. thin-layered chromatography detection method according to claim 1 it is characterised in that: in step a, the time of supersound process is
60min；The aperture of microporous filter membrane is 0.45 μm.
6. thin-layered chromatography detection method according to claim 1 it is characterised in that: in step b, the reference substance of astragaloside
Solution, the reference substance solution of oleanolic acid, the concentration of the reference substance solution of pinoresinol diglucoside are respectively 1mg/ml.
7. thin-layered chromatography detection method according to claim 1 it is characterised in that: in step c, described silica gel plate be silica gel
G lamellae.
8. thin-layered chromatography detection method according to claim 1 it is characterised in that: in step d, silica gel plate after colour developing,
On position corresponding with reference substance chromatograph, the speckle of test sample Chromatogram display same color.
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Address after: No. 259, Middle Section of Jinfu Road, Wenjiang District, Chengdu City, Sichuan Province, 611130 Patentee after: CHENGDU QIANKUN VETERINARY PHARMACEUTICAL CO.,LTD. Address before: 611130 Wenjiang District, Chengdu, Sichuan, Chengdu science and technology development park across the Taiwan Strait Jinfu Road Patentee before: CHENGDU QIANKUN VETERINARY PHARMACEUTICAL Co.,Ltd. |