CN104861047A - Ferritin-based monofunctionalized magnetic nanometer particle - Google Patents
Ferritin-based monofunctionalized magnetic nanometer particle Download PDFInfo
- Publication number
- CN104861047A CN104861047A CN201410066418.2A CN201410066418A CN104861047A CN 104861047 A CN104861047 A CN 104861047A CN 201410066418 A CN201410066418 A CN 201410066418A CN 104861047 A CN104861047 A CN 104861047A
- Authority
- CN
- China
- Prior art keywords
- ferritin
- dps
- nano particle
- monofunctionalized
- asymmetric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The invention discloses ferritin-based monofunctionalized magnetic nanometer particle. The ferritin-based monofunctionalized magnetic nanometer particle comprises a monofunctionalized ferritin shell and a magnetic nanometer particle core. The monofunctionalized ferritin shell is an asymmetric globulin with a tetrahedral structure. The asymmetric globulin mainly comprises one mutant Dps subunit and eleven wild Dps subunits. The Dps is a starvation-induced DNA binding protein. The ferritin-based monofunctionalized magnetic nanometer particle is prepared from biomacromolecule-protein as a template material, can be reconstructed easily, can be operated conveniently, is convenient for large-scale acquisition, has only one functional group and has an important meaning for follow-up controllable assembling.
Description
Technical field
The present invention is specifically related to a kind of single functional magnetic nano particle based on ferritin, belongs to field of nano biotechnology.
Background technology
Magnetic nanoparticle is the important component part in nano material, because of the distribution of sizes that it is special, magnetic nanoparticle possesses the effects such as surface and interface effect, small-size effect, quantum size effect, macroscopic quantum tunneling, so be widely used, relate to the every field such as machinery, electronics, optics, magnetics, chemistry and biology.And magnetic nano-particle be applied to Magnetic resonance imaging, Magneto separate, useful for drug delivery a prerequisite of the aspect such as carrier and tumor thermotherapy be that there is good superparamagnetism.Though non-surface modification has good superparamagnetism, because particle diameter is little, the impact of the large and Van der Waals force of specific surface area, particle itself is easy to assemble, poor stability, in vivo easily engulf by the abundant organ of the reticuloendothelial systems such as liver spleen, transformation period is short, makes it apply and is restricted.So one of key of applying in medical science of development magnetic nanoparticle is transformed into, to carry out modification to nano particle coated.
Development in recent years is for the modification of magnetic nanoparticle and coated series of novel and the effective strategy of providing.Amphipathic nature polyalcohol molecule can be modified in magnetic nanoparticle outside by these methods, or wrapping biological polymer, thus enhances biocompatibility, to cytotoxic, and greatly extends cycling time in the blood vessel.The biomacromolecule such as protein, DNA is natural nano material, their various structures, can self-replacation, highly homogeneous, is easy to artificial and handles and a large amount of preparation.DNA shows unique advantage in Nano-technology Development; Compared with DNA, the structural information that protein carries is abundanter, is thus expected to for nanotechnology provides service platform more flexibly.Wherein, albumen cage structure, such as viral capsid, ferritin, thermal shock albumen have been widely used in the coated of nano particle or as reaction vessel synthesizing magnetic nano particle.
But because the pattern of magnetic nanoparticle own and surface properties have Sphere symmetry, general method can only form uniform decorative layer, can not form anisotropic magnetic Nano structure.Recent years, report a series of effective to the asymmetric modification of magnetic nanoparticle, coated method successively, isotropic modified by magnetic nanoparticles, its itself can be become anisotropy, this bottleneck is broken through.But, obtain a kind of asymmetric magnetic nanoparticle, and their self-assembly of controlled manipulation on a molecular scale, and their assembling product of accurate analysis, remain challenging work.
Summary of the invention
In view of deficiency of the prior art, the object of the present invention is to provide a kind of single functional magnetic nano particle based on ferritin, it can realize single functionalization of magnetic nanoparticle on a molecular scale, make magnetic nanoparticle can as construction unit, for follow-up Controllable assembly provides better strategy and development space, and then more complicated, controlled and directed assembling can be carried out.
For achieving the above object, present invention employs following technical scheme:
A kind of single functional magnetic nano particle based on ferritin, comprise single functionalization ferritin shell and magnetic nanoparticle kernel, described single functionalization ferritin shell is the asymmetric sphaeroprotein with tetrahedral structure, described asymmetric sphaeroprotein forms primarily of 1 saltant type Dps subunit and 11 wild-type Dps subunits (being called for short wtDps), and described Dps is the DNA associated proteins of hungry induction.
Further, described saltant type Dps inserts a His-tag and section at the N end of wild-type Dps to have can being obtained by specific biotinylated 15 peptides of sequence shown in SEQ ID No.1, and can referred to as HBDps.
Further, the protein-bonded functional group of DNA of described hunger induction be contained by saltant type Dps subunit can by specific biotinylated 15 peptides, being separated group is His-tag.
Further, the external diameter of described single functionalization ferritin shell is 8-10nm, and internal diameter is 4.5-5nm.
Further, the mean thickness of described single functionalization ferritin shell is 2nm.
Further, described magnetic nanoparticle kernel comprises the ferric oxide nanometer particle that diameter is 2-3nm.
Compared with prior art, beneficial effect of the present invention comprises: by adopting biomacromolecule-protein as mould material, construct the single functional magnetic nano particle based on ferritin, it is easily transformed, be convenient to manipulation, convenience obtains in a large number, and should only have a functional group based on single functional magnetic nano particle of ferritin, there is great meaning to follow-up Controllable assembly.
Accompanying drawing explanation
Fig. 1 is the SDS-PAGE glue figure in the present invention one exemplary embodiments;
Fig. 2 is the transmission electron microscope figure of a kind of asymmetric protein nano particle utilizing the DBP assembling of hungry induction obtained in the present invention one exemplary embodiments;
Fig. 3 is the transmission electron microscope figure of the DNA associated proteins absorption AuNPs of unifunctional hunger induction shown in Fig. 2;
Fig. 4 is the transmission electron microscope figure that AuNPs is adsorbed in the positives contrast of the present invention one exemplary embodiments (the DNA associated proteins of saltant type hunger induction);
Fig. 5 is the transmission electron microscope figure that negative control in the present invention one exemplary embodiments (the DNA associated proteins of wild-type hunger induction) adsorbs AuNPs.
Fig. 6 is the transmission electron microscope figure (crossing by phospho-wolframic acid negative staining) of mineralising Iron oxide magnetic nanoparticles in asymmetric protein nano particle in the present invention one exemplary embodiments;
Fig. 7 is the transmission electron microscope figure (not crossing by phospho-wolframic acid negative staining) of mineralising Iron oxide magnetic nanoparticles in asymmetric protein nano particle in the present invention one exemplary embodiments;
Fig. 8 is the agarose-gel electrophoresis figure in the present invention one exemplary embodiments;
Fig. 9 is the nucleus magnetic resonance weighted imaging figure in the present invention one exemplary embodiments.
Embodiment
Purport of the present invention is to provide a kind of single functional magnetic nano particle based on ferritin, its cardinal principle is: the DBP utilizing biomacromolecule-hunger induction is service platform, by the genetic modification on DBP surface of inducing hunger, functional group/separation group is structurally coupled dexterously, then after transformed ferritin and the ferritin of wild-type fully being mixed in specific proportions, depolymerization is assembled again, the Controllable assembly of the DBP of hungry induction is realized by the method for control two kinds of protein ratio, obtain a kind of nano particle of asymmetric functionalization.Again according on asymmetric particle with separation group, the abstraction and purification of further realize target product.Finally be separated and obtain mineralising magnetic nanoparticle in the nano particle of asymmetric functionalization, and preferably adopt AuNPs(golden nanometer particle) its functional group is characterized, confirm that obtained product is the magnetic nanoparticle of single functionalization.
Concrete, this preparation method comprises the steps:
1) DBP that hunger is induced is carried out genetic modification, build with functional group and the carrier of protein mutant being separated group;
2) express in the vector built to organism, purifying also identifies the DBP mutant obtaining hungry induction;
3) this mutant is fully mixed with the ferritin of wild-type, then under the condition regulating pH, carry out albumen depolymerization and Hybrid assembling;
4) according to the feature purifying assembling product being separated group;
5) nano particle of the asymmetric functionalization obtained is carried out mineralising process, at granular center synthetic iron oxide magnetic nanoparticle.Both the magnetic nanoparticle of object product singly-bound had been obtained.
Be easier to understand the practicality of its substantive distinguishing features and institute's tool thereof for making the single functional magnetic nano particle based on ferritin of the present invention; constipation unification exemplary embodiments and Fig. 1-Fig. 9 are described in further detail structure of the present invention and embodiment below, but the following description about embodiment and explanation do not constitute any limitation scope.
step one,the DBP of structure, expression, the induction of purified mutant type hunger.Concrete steps are as follows:
The N end of the Loop of the DBP of hunger induction insert His-tag and can by shown in specific biotinylated 15 peptide GLNDIFEAQKIEWHE(SEQ ID No.1), be built into carrier PET32a-His-Dps(His-Dps); The exactness of goal gene sequence is determined through order-checking.PET32a-His-Dps Calcium Chloride Method is proceeded to E.coli Rosetta(DE3) competent cell, access in 5 mL LB test-tube culture mediums from picking mono-clonal flat board after being coated with dull and stereotyped (being added with penbritin and paraxin in flat board) at least 12 h, add penbritin (final concentration 100 μ g/mL) and paraxin (final concentration 68 μ g/mL), in 37 DEG C of constant temperature, 180 r/min overnight incubation.In 5 mL LB test-tube culture mediums (parallel 3 pipes) are transferred according to 1% (v/v) inoculum size, add corresponding microbiotic, in 37 DEG C of constant temperature, 180 r/min shaking culture 2.5 about h (OD600 is between 0.4 ~ 0.6), wherein 1 pipe is as blank (not adding inductor IPTG), other two Guan Jun add the IPTG that inductor final concentration is 1 mM, respectively after 25 DEG C and 37 DEG C continue inducing culture 10 h and 2 h, collected by centrifugation thalline, the expression of His-Dps is detected with SDS-PAGE, all can express under finding two kinds of temperature, also there is inclusion body to produce simultaneously, wherein 37 DEG C of inclusion bodys are more.Select 25 DEG C as inducing temperature when preparing albumen in a large number.
By E.coli Rosetta(DE3)/PET32a-His-Dps is inoculated in 5 mL LB test-tube culture mediums, adds corresponding microbiotic in 37 DEG C of constant temperature, 180 r/min overnight incubation.5 mL bacterium liquid were transferred in 500 mL LB triangular flasks in second day, add penbritin (final concentration 100 μ g/mL) and paraxin (final concentration 68 μ g/mL), after 37 DEG C of constant temperature, 180 r/min shaking culture 2.5 about h (OD600 is between 0.4 ~ 0.6), add the IPTG that inductor final concentration is 1 mM, vibration inducing culture 10 h is continued, collected by centrifugation thalline at 25 DEG C.Bacterial sediment binding buffer cleaning is once resuspended in the binding buffer of certain volume afterwards and carries out ultrasonication (condition: ultrasonic power 400W, work 4s, interval 4s, total ultrasonication time 60 min), supernatant liquor, with centrifugal 30 min of 12000 r/min, is splined on through the equilibrated Ni of binding buffer by the bacterium liquid after fragmentation
2+--NTA affinity column, then use the imidazoles of lower concentration (20mM, 40mM, 60mM, 120 mM) to wash post except foreigh protein removing successively, finally use elution buffer(500mM imidazoles) wash-out target protein.
HBDps and wtDps prepared exists with spherical nanoparticle form.The concentration of HBDps and wtDps is by test kit Bradford Protein Assay(dying method with coomassie brilliant blue) measure, step is carried out according to test kit specification sheets.Verify in conjunction with SDS-PAGE simultaneously.
step 2,the assembling of single function Dps and purifying.Concrete steps are as follows:
1. His-Dps (21KD) 0.224 mL (counting 0.57mg) be wtDps (18KD) 2mL (meter 16.24mg) and the concentration of 8.12mg/mL by concentration being 1.776 mg/mL mixes, 4 DEG C of 2h that vibrate at a slow speed;
2. diluting total protein liquid is 1mg/mL to final concentration, regulates pH value depolymerization and the asymmetric protein structure of Hybrid assembling again.Concrete steps are as follows:
I. protein liquid is loaded in dialysis tubing and put into 900mL water, in water, add 0.1M HCl with the speed of 1.5mL/min with syringe pump, until pH value is transferred to 2.Make up water to 1L, 4 DEG C of standing dialysis 12 h.The principle of this process be Dps under the condition of pH=2, protein monomer can be depolymerized to by the sphaeroprotein assembled.
II. configure 1 L phosphate buffered saline buffer (0.001M/L), regulate pH=2 with concentrated hydrochloric acid, put into above-mentioned dialysis tubing, in water, add 2M/L NaOH with the speed of 0.5mL/min with syringe pump, until pH value is transferred to 7, rear 4 DEG C of standing dialysis 12 h.The principle of this process be Dps when pH=2 modulates pH=7, can sphaeroprotein be reassembled into.
3. the product obtained contains the Dps of Hybrid assembling, His-Dps and wtDps sphaeroprotein and HBDps and wtDps monomer.Isolate asymmetric Hybrid assembling Dps nano particle, need following concrete steps:
I. in assembling liquid, add 5 × binding buffer 4.2 mL, now cumulative volume is 21. mL, is splined on assembling liquid through the equilibrated Ni of binding buffer
2+-NTA affinity column, can be adsorbed onto on chromatography column only with HBDps, use binding buffer, 15mM, the DNA associated proteins of the hunger induction of 30mM imidazoles removing non-specific adsorption on post, then use 100mM imidazoles wash-out list function Dps, then use 500-1000mM imidazoles wash-out with the sphaeroprotein of multiple His-Dps monomer.The principle of this process is: Ni
2+-NTA chromatography gel matrix is connected to a NTA(nitrilotriacetic acid, nitrilotriacetic acid), can with Ni ionic bond, produce stronger sequestering action between the 6-his amino acid of Ni ion and fusion rotein, thus separate with the histidine-tagged albumen of his-tag and other protein region.Therefore when the imidazole solution wash-out by high density time marquis (as 300 mM or higher), imidazoles just with the imidazole ring competition binding of protein his-tag, fusion rotein elutes from gel the most at last.
4., by single function Dps 0.05M Tris, the 0.5M NaCl under 100mM imidazoles wash-out, the damping fluid dialysis of glycerine 5%, pH=8, is diluted to more than 10000 times.
Single function Dps characterizes:
Whether the Dps having two kinds of method validations to obtain is single functionalization.Concrete grammar is as follows:
1. obtained target protein is run SDS-PAGE glue, because wild-type Dps molecular weight is 18KD, mutagenicity Dps molecular weight is 21KD, both are in different positions in SDS-PAGE glue, according to both gray-scale values at SDS-PAGE glue, both amount of substance ratio can be judged, judge whether target protein is single functionalization with this, as shown in Figure 1, both amount of substances are calculated according to the gray-scale value of wtDps and HBDps in single function Dps, calculate both ratios and be about 30:1, namely provable obtained target protein is the Dps of single functionalization really.
2. NTA(nitrilotriacetic acid, nitrilotriacetic acid) AuNPs that modifies of-Ni can efficiently be attached on the his-tag of HBDps, but and wild-type Dps but there is no specific binding.With NTA-Ni-AuNPs adsorption experiment, sample is characterized (Fig. 3), prepare positive control (Fig. 4) and negative control (Fig. 5) simultaneously.When AuNPs and the target protein amount of substance that obtains are 2:1, target protein is an absorption gold grain substantially only, and wild-type Dps can not ADSORPTION OF GOLD particle, and full-mutant Dps then can adsorb the gold grain of 3-6 number not grade.This result illustrates: only comprise the monomer that has the His-Dps of his-tag in target protein, all the other 11 is the monomer of wild-type Dps.
step 3,synthetic iron oxide magnetic-particle in asymmetric protein nano particle.Concrete steps:
1. by above-mentioned purpose albumen 4.6 ml(330 μ g/mL) and 15mL 0.1M NaCl add be full of N2 protection jacketed reactor in, this reactor is connected with an autotitrator (TITRINO, AG is led in Switzerland ten thousand) and thermometer.The pH value of reaction mixture is regulated to be 8.5 with the NaOH of 30mM/L.Reactor is fixed on the aobvious constant temperature electric heating of SHT type stirring to put, is heated with stirring to 65 DEG C.
2. add freshly prepared 0.1mL 5mM/L ferrous ammonium sulphate and 0.075mL 2.5mM/L hydrogen peroxide, and regulate the pH value of reaction mixture to be 8.5 with the NaOH of 30mM/L, reaction 5min, repeats 8 times, finally reacts 10min again.
3. by product cool to room temperature, centrifugal 12000rpm, 30min.With the metre filter supernatant liquor of 0.2 μm, the lower liquid of filter namely for the purpose of product: the magnetic nanoparticle of single functionalization.
The sign of magnetic nanoparticle, namely verify in single function Dps whether comprise magnetic particle, have following methods:
First the sample crossed as Fig. 6 phospho-wolframic acid negative staining obviously can find out the particle at albumen and albumen center under Electronic Speculum, and through phospho-wolframic acid negative staining not as shown in Figure 7, can only see that diameter is at the small-particle of 2-3nm one by one.This proves that there is particle in albumen inner chamber ore deposit.
Whether ferric ion runs into ferrous cyanogen root can produce blue ferriferro cyanide precipitation, so can verify in albumen containing ferric oxide by prussian blue staining method.As shown in Figure 8: a is the agarose gel by Prussian blue dye, b contaminates have the agarose gel of same control sample with Xylene Brilliant Cyanine G.Wherein a3, b3 are the single function Dps not having mineralising; A2, b2 are the wt-Dps of mineralising, a1, b1 are single function Dps of mineralising, can find out, do not have the ferritin of mineralising but can not can only be dyed to Prussian blue by coomassie brilliant blue staining, and all by Maas light blue and Prussian bluely can dye blueness through the wild-type ferritin of mineralising and single function ferritin.In albumen, really ferric oxide nanometer particle is defined after mineralising is described.
Finally that NMR signal detection is carried out to sample.As shown in Figure 9: 2,3,4 holes of T1, T2 weighted imaging gray-scale map are plain buffer respectively, single function Dps(1.5 μm/L of 2 × mineralising), single function Dps(0.75um/l of 1 × mineralising).1 hole is irrelevant sample.Result shows that single function Dps of mineralising can more obviously shorten the T1 relaxation time, also can obviously shorten the T2 relaxation time simultaneously, to sum up can judge, that be combined at asymmetric single function Dps chats is ultra-fine paramagnetic iron oxide (Ustrasmall superparamagnetic iron oxide, USPIO).
The application prospect of the magnetic label probe imaging that USPIO contrast medium mediates at acceptor etc. is by the extensive attention of people.So-called magnetic label probe refers to paramagnetic particles to be combined with specific receptors, antibody or gene in some way and proceeds in cell, because this paramagnetic substance is directly proportional be connected amount of material in intracellular accumulation, therefore, the MR signal distributions that paramagnetic substance causes represents the distribution of acceptor, antibody or gene, and by the part of paramagnetic label, under receptor-mediated, produce specificity and concentrate, reach the object of selective reinforcement and then imaging.The present invention obtain the single functional magnetic nano particle based on ferritin, namely can be used as magnetic label probe, the functionalization subunit of single function Dps add suitable ligand, the object of selective reinforcement signal and then imaging can be reached.
Below be only the embodiment in the numerous embody rule example of the present invention, protection scope of the present invention is not constituted any limitation.The technical scheme that all employing equivalents or equivalence are replaced and formed, all drops within rights protection scope of the present invention.
<110> Suzhou Institute of Nano-tech. and Nano-bionics, Chinese Academy of Sciences
<120> is based on single functional magnetic nano particle of ferritin
<160> 1
<210> 1
<211> 15
<212> PRT
<213> artificial sequence
<400> 1
Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu
1 5 10 15
Claims (6)
1. the single functional magnetic nano particle based on ferritin, it is characterized in that comprising single functionalization ferritin shell and magnetic nanoparticle kernel, described single functionalization ferritin shell is the asymmetric sphaeroprotein with tetrahedral structure, described asymmetric sphaeroprotein forms primarily of 1 saltant type Dps subunit and 11 wild-type Dps subunits, and described Dps is the DNA associated proteins of hungry induction.
2. the single functional magnetic nano particle based on ferritin according to claim 1, it is characterized in that described saltant type Dps be the N-terminal of wild-type Dps insert that a Histag and section has a sequence shown in SEQ ID No.1 can by specific biotinylated 15 peptides.
3. the single functional magnetic nano particle based on ferritin according to claim 2, it is characterized in that, the protein-bonded functional group of DNA of described hunger induction is by specific biotinylated 15 peptides, can be separated group and comprising His-tag contained by saltant type Dps subunit.
4. the single functional magnetic nano particle based on ferritin according to claim 1, is characterized in that, the external diameter of described single functionalization ferritin shell is 8-10nm, and internal diameter is 4.5-5.5nm.
5. single functionalization ferritin shell according to claim 1, is characterized in that, the mean thickness of described single functionalization ferritin shell is 2nm.
6. the single functional magnetic nano particle based on ferritin according to claim 1, it is characterized in that, described magnetic nanoparticle kernel comprises the ferric oxide nanometer particle that diameter is 2-3nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410066418.2A CN104861047B (en) | 2014-02-26 | 2014-02-26 | Single function magnetic nanoparticle based on ferritin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410066418.2A CN104861047B (en) | 2014-02-26 | 2014-02-26 | Single function magnetic nanoparticle based on ferritin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104861047A true CN104861047A (en) | 2015-08-26 |
| CN104861047B CN104861047B (en) | 2018-03-20 |
Family
ID=53907273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410066418.2A Active CN104861047B (en) | 2014-02-26 | 2014-02-26 | Single function magnetic nanoparticle based on ferritin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104861047B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283251A (en) * | 2019-04-18 | 2019-09-27 | 中国科学院武汉病毒研究所 | A kind of DNA binding protein and application of the hungry induction of cross-film |
| WO2021233244A1 (en) * | 2020-05-19 | 2021-11-25 | 昆山新蕴达生物科技有限公司 | Ferritin heavy chain subunit-based conjugates and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703763A (en) * | 2002-10-04 | 2005-11-30 | 纳磁股份有限公司 | Magnetic nanoparticles and method of fabrication |
| CN102321590A (en) * | 2011-07-29 | 2012-01-18 | 中国科学院苏州纳米技术与纳米仿生研究所 | Preparation method of asymmetric virus nanoparticles |
| CN102711794A (en) * | 2010-01-04 | 2012-10-03 | Kj生物科学有限公司 | Dps fusion proteins for use in vaccines and diagnostics |
-
2014
- 2014-02-26 CN CN201410066418.2A patent/CN104861047B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703763A (en) * | 2002-10-04 | 2005-11-30 | 纳磁股份有限公司 | Magnetic nanoparticles and method of fabrication |
| CN102711794A (en) * | 2010-01-04 | 2012-10-03 | Kj生物科学有限公司 | Dps fusion proteins for use in vaccines and diagnostics |
| CN102321590A (en) * | 2011-07-29 | 2012-01-18 | 中国科学院苏州纳米技术与纳米仿生研究所 | Preparation method of asymmetric virus nanoparticles |
Non-Patent Citations (2)
| Title |
|---|
| MARTA ALMIR6N ET AL.: "A novel DNA-binding protein", 《GENES DEV》 * |
| 赵紫来等: "氧化铁磁性纳米粒子的制备、表面修饰及在分离和分析中的应用", 《化学进展》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283251A (en) * | 2019-04-18 | 2019-09-27 | 中国科学院武汉病毒研究所 | A kind of DNA binding protein and application of the hungry induction of cross-film |
| CN110283251B (en) * | 2019-04-18 | 2023-01-13 | 中国科学院武汉病毒研究所 | Transmembrane starvation-induced DNA binding protein and application thereof |
| WO2021233244A1 (en) * | 2020-05-19 | 2021-11-25 | 昆山新蕴达生物科技有限公司 | Ferritin heavy chain subunit-based conjugates and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104861047B (en) | 2018-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pareek et al. | Formation and characterization of protein corona around nanoparticles: a review | |
| Rana et al. | Engineering the nanoparticle–protein interface: applications and possibilities | |
| Aili et al. | Bioresponsive peptide–inorganic hybrid nanomaterials | |
| Wang et al. | The synthesis and bio-applications of magnetic and fluorescent bifunctional composite nanoparticles | |
| Hu et al. | Colloidal particles for cellular uptake and delivery | |
| Corr et al. | Multifunctional magnetic-fluorescent nanocomposites for biomedical applications | |
| Huang et al. | Biochemical and biomedical applications of multifunctional magnetic nanoparticles: a review | |
| Wang et al. | Advances in epitope molecularly imprinted polymers for protein detection: a review | |
| Zhou et al. | Ni2+-BSA directional coordination-assisted magnetic molecularly imprinted microspheres with enhanced specific rebinding to target proteins | |
| KR101975754B1 (en) | Peptides capable of silica deposition and use thereof | |
| CN106970215B (en) | A kind of preparation method of the Fe3O4@PEG@SiO2 artificial antibodies of detection thifensulfuronmethyl | |
| CA2812208C (en) | Synthesis of fluorescent noble metal nanoparticles | |
| Terada et al. | Nanodiamonds for bioapplications–specific targeting strategies | |
| KR20130035985A (en) | Peptides capable of silica deposition and use thereof | |
| Takahashi et al. | Surface modification of magnetic nanoparticles using asparagines-serine polypeptide designed to control interactions with cell surfaces | |
| US20160303256A1 (en) | Methods and compositions for self-assembly system of nanoparticles and microparticles for multi-targeting specificity | |
| JP2018514794A5 (en) | ||
| Chu et al. | Preparation of quantum dot-coated magnetic polystyrene nanospheres for cancer cell labelling and separation | |
| Andrade et al. | Coating nanomagnetic particles for biomedical applications | |
| Aseri et al. | Magnetic nanoparticles: Magnetic nano-technology using biomedical applications and future prospects | |
| Slegerova et al. | Nanodiamonds as intracellular probes for imaging in biology and medicine | |
| Huang et al. | Fluorescent-magnetic multifunctional nanoparticles for imaging and drug delivery | |
| Wang et al. | Assembly of multivalent protein ligands and quantum dots: a multifaceted investigation | |
| CN104903706A (en) | Metal-containing semiconducting polymer dots | |
| CN104861047A (en) | Ferritin-based monofunctionalized magnetic nanometer particle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |