CN104855341B - 小杆线虫在防治核桃云斑天牛中的应用 - Google Patents
小杆线虫在防治核桃云斑天牛中的应用 Download PDFInfo
- Publication number
- CN104855341B CN104855341B CN201510275635.7A CN201510275635A CN104855341B CN 104855341 B CN104855341 B CN 104855341B CN 201510275635 A CN201510275635 A CN 201510275635A CN 104855341 B CN104855341 B CN 104855341B
- Authority
- CN
- China
- Prior art keywords
- rhabditis axei
- walnut
- preventing
- tumian
- rhabditis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000244173 Rhabditis Species 0.000 title claims abstract description 36
- 241001042196 Batocera horsfieldi Species 0.000 title claims abstract description 32
- 235000020234 walnut Nutrition 0.000 title claims abstract description 32
- 235000009496 Juglans regia Nutrition 0.000 title claims abstract description 30
- 240000007049 Juglans regia Species 0.000 title 1
- 241000758789 Juglans Species 0.000 claims abstract description 29
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 230000012447 hatching Effects 0.000 claims abstract description 5
- 238000005520 cutting process Methods 0.000 claims description 9
- 241001481710 Cerambycidae Species 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 5
- 239000007900 aqueous suspension Substances 0.000 claims description 2
- 238000011161 development Methods 0.000 claims description 2
- 230000018109 developmental process Effects 0.000 claims description 2
- 210000003608 fece Anatomy 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 238000012937 correction Methods 0.000 abstract description 5
- 241000244206 Nematoda Species 0.000 description 16
- 230000013872 defecation Effects 0.000 description 8
- 241000144065 Oscheius Species 0.000 description 7
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 210000004681 ovum Anatomy 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 241000254173 Coleoptera Species 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001600609 Equus ferus Species 0.000 description 2
- 241000758791 Juglandaceae Species 0.000 description 2
- 208000000291 Nematode infections Diseases 0.000 description 2
- 241000258634 Oscheius chongmingensis Species 0.000 description 2
- 240000005809 Prunus persica Species 0.000 description 2
- 235000006040 Prunus persica var persica Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000000205 computational method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000282817 Bovidae Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000244178 Rhabditidae Species 0.000 description 1
- 241000545593 Scolytinae Species 0.000 description 1
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及小杆线虫在防治核桃云斑天牛中的应用。小杆线虫R.(O.)chongmingensis Tumian(简称Tumian)可防治蛀干害虫。本发明人将小杆线虫Tumian制成制剂施用于核桃园中，结果表明小杆线虫Tumian防治核桃云斑天牛的效果显著，防治幼虫效果达90％(校正有效率91.4％)以上，阻止卵孵化效果近70％(68.4％)。
Description
技术领域
本发明涉及一种生物防治技术领域，特别是涉及利用一种小杆线虫Rhabditis(Oscheius)chongmingensis Tumian防治核桃云斑天牛的技术。
背景技术
云斑天牛(Batocera horsfieldi)又称云斑白条天牛，属鞘翅目(Coleoptera)天牛科(Cerambycidae)，是我国重大林木蛀干害虫，在核桃树上危害尤为严重。主要以幼虫在树干木质部取食钻蛀危害，并以幼虫和成虫在蛀道内和蛹室中越冬，形成大量蛀道，严重时造成树木死亡或风折，从而使之失去经济价值和生态价值。
长期以来对于此类害虫的防治主要靠人工钩杀和蛀道内注入高浓度、高毒性化学农药。但这些方法效果并不理想，而且对施药人员和生态环境毒性较大。
小杆属线虫资源丰富，分布广泛，易于获得，易于培养。传统上认为小杆属Rhabditis(Oscheius)线虫是食细菌类线虫，国外尚未见小杆线虫用于防治害虫的报道，国内仅有本发明人用小杆线虫Rhabditis spp防治地下害虫蛴螬的报道，迄今为止国内外尚未见用该小杆线虫Rhabditis(Oscheius)chongmingensis Tumian防治核桃云斑天牛的报道。
发明内容
本发明针对上述领域的空白，提供小杆线虫R.(Oscheius)chongmingensisTumian在防治核桃云斑天牛中的应用，以解决生产上的难题，拓宽生物防治途径。
小杆线虫R.(O.)chongmingensis Tumian(简称Tumian)在防治蛀干害虫中的应用。
所述蛀干害虫为天牛。
所述天牛为核桃云斑天牛。
所述应用是将所述小杆线虫制剂在植物生长发育期施于被天牛危害的树干、树枝刻槽部和排粪孔内。
所述植物为核桃树，所述天牛为核桃云斑天牛。
所述防治是抑制云斑天牛卵孵化或杀死云斑天牛幼虫或成虫。
所述小杆线虫制剂为小杆线虫的悬浮水溶液，浓度为250-8000条/孔，施用量为2-4mL/蛀孔。
所述小杆线虫制剂的浓度为2000-4000条/mL。
本发明提供了一种新的防治云斑天牛的方法，采用本发明人从内蒙棉田土壤中采集到的小杆线虫Tumian制成制剂施用于核桃园中，结果表明小杆线虫Tumian防治核桃云斑天牛的效果显著，防治幼虫效果达90％(校正有效率91.4％)以上，阻止卵孵化效果近70％(68.4％)。
附图说明
图1各剂量(IJs/mL)小杆线虫R.chongmingensis Tumian对核桃云斑天牛卵的校正有效率。图2各剂量(IJ/mL)小杆线虫R.chongmingensis Tumian对核桃云斑天牛幼虫的校正有效率。
具体实施方式
现结合实施例对本发明作进一步的详述。
本发明实施例虽然只是应用于核桃云斑天牛的防治，但对危害其它林木果树的及其它蛀干害虫(如吉丁虫、小蠹虫等)来说，基于同样的原理，小杆线虫Tumian同样适用于其它天牛危害和蛀干害虫危害的果园和林地。唯有不同的是实用技术，既不同蛀干害虫在不同地区、不同寄主上的危害时期和部位深浅不同，需根据生物学特性而调整。
实施例一、田间防治核桃云斑天牛卵的效果案例
1材料与方法
1.1试验材料
供试药剂：小杆线虫Rhabditis(Oscheius)chongmingensis Tumian(Liu Qizhi,Zdenˇek M.Zhang Lijuan,Vladimír P.and Dong Lamei.2012.Re-description ofOscheius chongmingensis(Zhang et al.,2008)(Nematoda:Rhabditidae)and itsentomopathogenicity.Nematology,14(2):139-149.),本申请第一发明人从内蒙棉田土壤中采集、分离、纯化、扩繁所得，可对外公开发放。收集后的线虫悬浮在水溶液中，在4℃保存，20天内施用。线虫施用浓度如下述试验方法。
1.2试验方法
2014年7-9月在贵州省赫章县野马川镇苏噶村随机选取被云斑天牛严重危害的核桃园，不进行其它防治。在核桃树上标记有天牛卵的新刻槽。
将线虫R.chongmingensis Tumian分别配成250IJs/mL、500IJs/mL、1000IJs/mL、2000IJs/mL、4000IJs/mL五个浓度梯度。每个处理设置3个重复(即3棵核桃树)，每个重复(每棵核桃树上)的刻槽数20～100个不等，标记所有刻槽。每个刻槽表层涂施1-2mL某一种浓度的线虫粘稠液。对照施用等量清水粘稠液。每7天观察并记录一次有粪排除的刻槽数后清除虫粪，共观察7次，至第49天。
1.3计算方法
按下列公式计算：
卵的有效率％＝无虫粪率％＝[1-(排粪刻槽数/总刻槽数)]×100％
校正有效率％＝(处理无虫粪率-对照无虫粪率)/(100％-对照无虫粪率)×100％
2结果与分析
2.1防治核桃云斑天牛卵的有效率
从图1看出：供试线虫O.chongmingensis Tumian对核桃云斑天牛卵的抑制效果在4000IJs/mL线虫剂量下达54.7～68.4％(7天——49天)。从整体趋势来看，线虫侵染时间越长，孵化抑制率越高。
实施例二、田间防治核桃云斑天牛幼虫的效果案例
1材料与方法
1.1试验材料
供试药剂：小杆线虫Rhabditis(Oscheius)chongmingensis Tumian，本第一发明人从内蒙棉田土壤中采集、分离、纯化、扩繁所得，可对外公开发放。收集后的线虫悬浮在水溶液中，在4℃保存，20天内施用。线虫施用浓度如下述试验方法。
1.2试验方法
2014年7-9月在贵州省赫章县野马川镇苏噶村随机选取被云斑天牛严重危害的核桃园，不进行其它防治。在核桃树上标记幼虫排粪孔。
将线虫R.chongmingensis Tumian分别配成250IJs/mL、500IJs/mL、1000IJs/mL、2000IJs/mL、4000IJs/mL五个浓度梯度。每个处理设置3个重复(即3棵核桃树)，每个重复(每棵核桃树上)的排粪孔数20～100个不等，标记所有排粪孔。每个排粪孔注入2-4mL某一种浓度的线虫粘稠液。对照注入等量清水粘稠液。每7天观察并记录一次有粪排除的排粪孔数后清除虫粪，共观察4次，至第28天。
1.3计算方法
按下列公式计算：
幼虫的有效率％＝无虫粪率％＝[1-(排粪孔数/总孔数)]×100％
校正有效率％＝(处理无虫粪率-对照无虫粪率)/(100％-对照无虫粪率)×100％
2结果与分析
2.1防治核桃云斑天牛幼虫的有效率
从表1看出：供试线虫Tumian对核桃云斑天牛幼虫具有很高的侵染力，防治效果达75.3～93.7％。施用该线虫浓度为250IJs/mL时，7-28天的有效率达75.3～80.1％；施用500IJs/mL时，有效率达78.4～84.4％；施用1000IJs/mL时，有效率达81.8～88.5％；施用2000IJs/mL时，有效率达83.5～90.3％；线虫施用为4000IJs/mL时，有效率达84.5～93.7％。
表1 小杆线虫R.chongmingensis Tumian侵染核桃云斑天牛幼虫有效率
2.2防治核桃云斑天牛幼虫的校正有效率
从图2看出：供试线虫Tumian对核桃云斑天牛幼虫的校正有效率在4000IJs/mL线虫剂量下第28天达91.4％。
从整体趋势来看，线虫侵染剂量越大、侵染时间越长，对幼虫的防治有效率越高。
Claims (4)
1.小杆线虫R.(O.)chongmingensis Tumian在防治蛀干害虫中的应用，所述蛀干害虫为核桃云斑天牛；
所述应用是将小杆线虫制剂在植物生长发育期施于被天牛危害的树干、树枝刻槽部和排粪孔内，所述植物为核桃树。
2.根据权利要求1所述的应用，所述防治是抑制云斑天牛卵孵化或杀死云斑天牛幼虫。
3.根据权利要求1所述的应用，所述小杆线虫制剂为小杆线虫的悬浮水溶液，浓度为250-8000条/mL，施用量为2-4mL/蛀孔。
4.根据权利要求3所述的应用，所述小杆线虫制剂的浓度为2000-4000条/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510275635.7A CN104855341B (zh) | 2015-05-26 | 2015-05-26 | 小杆线虫在防治核桃云斑天牛中的应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510275635.7A CN104855341B (zh) | 2015-05-26 | 2015-05-26 | 小杆线虫在防治核桃云斑天牛中的应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104855341A CN104855341A (zh) | 2015-08-26 |
| CN104855341B true CN104855341B (zh) | 2017-08-29 |
Family
ID=53901873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510275635.7A Expired - Fee Related CN104855341B (zh) | 2015-05-26 | 2015-05-26 | 小杆线虫在防治核桃云斑天牛中的应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104855341B (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173124A (zh) * | 2017-06-05 | 2017-09-19 | 湖北省林业科学研究院 | 一种核桃白条天牛防治方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5694883A (en) * | 1993-07-23 | 1997-12-09 | Kubota Corporation | Nematoda cultivating method |
| CN1875718A (zh) * | 2006-05-17 | 2006-12-13 | 刘奇志 | 小杆线虫在防治蛴螬中的应用 |
| CN102640731A (zh) * | 2012-04-17 | 2012-08-22 | 中国科学院东北地理与农业生态研究所 | 一种提高昆虫病原线虫侵染能力的方法 |
-
2015
- 2015-05-26 CN CN201510275635.7A patent/CN104855341B/zh not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5694883A (en) * | 1993-07-23 | 1997-12-09 | Kubota Corporation | Nematoda cultivating method |
| CN1875718A (zh) * | 2006-05-17 | 2006-12-13 | 刘奇志 | 小杆线虫在防治蛴螬中的应用 |
| CN102640731A (zh) * | 2012-04-17 | 2012-08-22 | 中国科学院东北地理与农业生态研究所 | 一种提高昆虫病原线虫侵染能力的方法 |
Non-Patent Citations (4)
| Title |
|---|
| 园林蛀干害虫的生物防治;严彩霞等;《河北林果研究》;20101215;第25卷(第4期);第389-393页 * |
| 小杆线虫Rhabditis sp.品系Tumian的初步鉴定、生物学及应用评价;曹海锋;《中国农业大学硕士学位论文》;20070723;摘要，第10、43页 * |
| 小杆线虫Rhabditis(Oscheius)sp.生物学特性测定;张丽娟等;《安徽农业科学》;20100101;第38卷(第1期);第212-213页 * |
| 李宁等.11个昆虫病原线虫品系对光肩星天牛的致死能力.《东北林业大学学报》.2011,第39卷(第8期),第68-69页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104855341A (zh) | 2015-08-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Di Marco et al. | Applications of Trichoderma to prevent Phaeomoniella chlamydospora infections in organic nurseries | |
| JP2008202051A (ja) | 栽培した沈香 | |
| CN104823626A (zh) | 一种防控柑桔黄龙病同时收获有机柑桔的农业生态技术 | |
| KR101033270B1 (ko) | 소나무 재선충 방제용 조성물 및 이를 이용한 방제 방법 | |
| CN105076213A (zh) | 一种应用昆虫病原线虫与化学农药联合防治韭蛆的方法 | |
| Akinlabi et al. | Regeneration, cultivation, and sustenance of bamboo | |
| CN104813877A (zh) | 樱桃异小杆线虫在防治核桃云斑天牛中的应用 | |
| Vinodhini et al. | Effect of plant extracts on root-knot nematode meloidogyne incognita infecting tomato | |
| CN104855341B (zh) | 小杆线虫在防治核桃云斑天牛中的应用 | |
| Harrell | Imidacloprid concentrations in green ash (Fraxinus pennsylvanica) following treatments with two trunk-injection methods | |
| Mahapatro et al. | Indigenous approaches for the management of termite and white grub in upland rice | |
| Karami et al. | Integrated management of the charcoal disease by silviculture, chemical and biological methods in forest parks | |
| Mani et al. | Root mealybugs and their management in horticultural crops in India | |
| Ananthakrishnan et al. | Ecological Entomology: Insect Life in Odd Environments | |
| CN107668077A (zh) | 一种含氟吡菌酰胺的杀线虫组合物 | |
| Sarpong | In vitro evaluation of the effect of selected plant extracts on the Phytophthora fungus causing disease in MD2 variety of pineapple in the central region of Ghana | |
| Parajuli et al. | Causal agents responsible for the die-back of Dalbergia sissoo in Nepal's eastern Tarai | |
| CN100360037C (zh) | 小杆线虫Rhabditis（Oscheius）spp.在防治植物线虫中的应用 | |
| Meddich et al. | Use of Olive Mill Wastewaters as Bio-Insecticides for the Control of Potosia Opaca in Date Palm (Phoenix dactylifera L.) | |
| Audley | Assessment of Pityophthorus juglandis colonization characteristics and implications for further spread of thousand cankers disease | |
| Javaid | Research on shisham (Dalbergia sissoo Roxb.) decline in Pakistan–a review | |
| Chowdhury | Production of agarwood through aeration method into the agar tree | |
| Shonga et al. | Cassava scale: a new threat for a food security crop in Ethiopia | |
| CN109362814A (zh) | 一种防治果园虫害的生物杀虫剂及其制备方法和使用方法 | |
| Taha | Effect of Intercropping of Nematotoxic Plants on Root-Knot Nematode, Meloidogne incognita Infecting some Vegetable Plants in the Screenhouse |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170829 Termination date: 20190526 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |