CN104849473A - Microalbuminuria detection kit and preparation thereof - Google Patents
Microalbuminuria detection kit and preparation thereof Download PDFInfo
- Publication number
- CN104849473A CN104849473A CN201510227239.7A CN201510227239A CN104849473A CN 104849473 A CN104849473 A CN 104849473A CN 201510227239 A CN201510227239 A CN 201510227239A CN 104849473 A CN104849473 A CN 104849473A
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- reagent
- malb
- latex
- antibody
- detection kit
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/34—Genitourinary disorders
- G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
Abstract
The invention provides a microalbuminuria (mAlb) detection kit, which is based on a latex enhanced turbidimetric immunoassay method, is a liquid double reagent, namely reagent R1 and reagent R2, wherein the reagent R1 is a reactant and the reagent R2 is a solution containing albumin immuno latex particles, and the detection kit is characterized by crosslinking by applying a chemical process, and covalently crosslinking a goat anti-human albumin antibody (also called goat anti-human mAlb antibody) with carboxylated polystyrene latex through water-soluble carbodiimide (EDC) and H-hydroxy succinimide (NHS), so as to form an mAlb antibody latex reagent. The reagent has the advantages of high sensitivity, strong specificity and simple preparation, and is worthy of further promotion.
Description
Technical field:
The invention belongs to biological technical field, be specifically related to a kind of microdose urine protein (mAlb) detection kit and preparation thereof.
Background technology:
Microalbuminuria refers to and occur microalbumin in urine.Albumin is the normal protein matter in a kind of blood, but only occurs minute quantity albumin in urine in physiological conditions.Microalbuminuria reflection renal abnormality leaky protein.Diabetic nephropathy, hypertension, pre-eclampsia are more common in increasing of microdose urine protein, are the early stage sensitive indicators of injury of kidney.The microdose urine protein which kind of disease causes is all the damage of the intrinsic cell of kidney caused because initial reason is different, and the structure of the intrinsic cell of kidney is changed, and function changes with the change of structure, the embodiment in urine.When discovery microdose urine protein is within the scope of 20mg/L ~ 200mg/L, routine urinalysis Urine proteins be shown as feminine gender (-) or (+-), just belong to microalbuminuria, illustrate that kidney damages.And when in urinating, microalbumin is more than 200mg/L, routine urinalysis test urine protein positive (+) ~ (+++), now prove that body has a large amount of albumin and spills, Hypoproteinemia may be there is, development of renal disease only has one step away from the irreversible phase, if cured not in time, Uremic will be entered.Clinical examination content generally comprises immune dysfunction assessment, and inflammatory conditions is monitored, cardiovascular risk assessment and the various aspects such as rheumatoid arthritis and streptococcal infection.
Microalbuminuria is also the sign that whole vascular system changes, and can think " window " of arterial disease, because it is the Symptoms at Primary Stage that kidney and cardiovascular system change.
Microdose urine protein is by immune turbidimetry, and immunofluorescence technique, radioimmunology, the multiple method such as enzyme immunoassay measures.The shortcomings such as radioimmunology susceptibility is high, high specificity, but has radioactive contamination, and reagent storage life is short.The maximum urine microalbumin detection method of domestic current employing is still ELISA method, but the operation steps of enzyme linked immunosorbent assay is many, therefore repeatability is poor.Albumin sensitization Carboxylated Polystyrene latex, sets up Latex agglutination inhibition to measure microdose urine protein, has quick, special, easy, inexpensive advantage.
Summary of the invention:
The object of the invention is to, a large amount of preparation mAlb antibody latex reagent, uses the mAlb antibody latex reagent obtained to prepare microdose urine protein detection kit.
Technical scheme of the present invention: applied chemistry method is cross-linked, goat-anti people mAlb antibody and Carboxylated Polystyrene latex covalent cross-linking is made by water-soluble carbodiimide (EDC) and N-hydroxy-succinamide (NHS), preparation mAlb antibody latex reagent, utilize the mAlb antibody latex reagent obtained, prepare microdose urine protein detection kit.
In the present invention, comprise the preparation of mAlb antibody latex reagent and the preparation of microdose urine protein detection kit.Concrete operations are as follows:
The preparation of mAlb antibody latex reagent:
1. the preparation of relevant buffers
The preparation of 0.01mol/L, PH7.2 PBS damping fluid: get 800ml deionized water dissolving 8g sodium chloride, 0.2g potassium chloride, 1.44g disodium-hydrogen and 0.24g potassium dihydrogen phosphate, 0.1% hydrochloric acid adjusts PH to 7.2, is settled to 1L, 15psi moist heat sterilization 20min with deionized water.
The preparation of 0.01mol/L, PH7.6 PBS damping fluid: get 800ml deionized water dissolving 8g sodium chloride, 0.2g potassium chloride, 1.44g disodium-hydrogen and 0.24g potassium dihydrogen phosphate, 0.1% hydrochloric acid adjusts PH to 7.6, is settled to 1L, 15psi moist heat sterilization 20min with deionized water.
The preparation of 2.mAlb antibody latex reagent
Raw material: be 200nm at particle diameter, concentration is in 10% Carboxylated Polystyrene latex 10ml, adds 0.01mol/L, PH7.2 phosphate buffer (PBS) 10ml, NHS 20mg and EDC 60mg, after room temperature stirs 30min, 37 DEG C of waters bath with thermostatic control react 1 hour, 2-8 DEG C of centrifugal (11000rpm, 30min), incline supernatant, returns to original volume with 0.01mol/L, PH7.2 phosphate buffer (PBS).Add 500mg/L goat-anti people mAlb antibody-solutions 2.0ml, NHS 30mg and EDC120mg, after room temperature stirs 30min, 37 DEG C of waters bath with thermostatic control react 2 hours, add 0.4mol/L, PH8.2 glycine solution 2.0ml reaction 20min and carry out cancellation.2-8 DEG C of centrifugal (11000rpm, 30min), incline supernatant, with 0.01mol/L, after PH7.6 phosphate buffer (PBS) washs 2 times, being made into concentration with same damping fluid is 1% emulsion reagent, anticorrosion with 0.1% (w/v) sodium azide solution, is mAlb antibody latex reagent.
The preparation of microdose urine protein detection kit:
This detection kit comprises reagent R1 and R2, and concrete composition is as follows:
The Cleaning Principle of this detection kit is by antibody linked in present latex particulate for goat-anti people mAlb, with the microalbumin generation antigen-antibody reaction in urine sample to be measured, cause microparticle agglutination, form certain turbidity, under 340nm wavelength, by the calibration object contrast processed equally, quantitatively detect the content of microalbumin in sample.
The use of microdose urine protein detection kit:
1) detecting instrument: the Biochemical Analyzer with 340nm wavelength, 37 DEG C of thermostats.
2) sample to be tested: urine, 2-8 DEG C of Absorbable organic halogens one day, is preferably urina sanguinis, and centrifugal (3000rpm/min) 10 minutes is stand-by.
3) basic parameter is measured:
| Predominant wavelength | 340nm | Temperature of reaction | 37℃ |
| Analysis type | End-point method | Reagent sample ratio | 20∶1 |
| Reaction time | 10 minutes | Type of calibration | Logit-4P/Spline |
4) concrete trace routine:
5) result of calculation: mAlb (mg/L)=Cs × Δ A in sample
t/ Δ A
s
In formula: Δ A
twith the sample hose absorbance of blank tube absorbance for contrast;
Δ A
swith the calibration tube absorbance of blank tube absorbance for contrast;
The concentration of mAlb in Cs calibration solution
6) reference range: 0-22.5mg/L.
7) precision: CV≤5% in batch; Relative extreme difference≤10% between batch.
8) accuracy: relative deviation < 10%.
9) range of linearity: should 200mg/L be reached, correlation coefficient r >=0.990.
Accompanying drawing illustrates:
Fig. 1: adopt reagent of the present invention and commercial reagent A respectively, adopts Olympus 5400 automatic clinical chemistry analyzer, to 50 parts of urine specimens (comprising normal and exceptional sample), measures, and carry out correlation analysis to measured value by each autoregressive parameter.The measured value of what wherein X-axis represented is reagent of the present invention, the measured value of what Y-axis represented is commercial reagent A.Related coefficient: r
2=0.9960, linear equation is: y=1.005x+0.041.
Embodiment:
Embodiment
Reagent of the present invention compares with the performance index of commercial reagent A:
1) precision measures: same sample continuous drawing measures for 20 times, calculates measured value mean, standard deviation and the coefficient of variation,
Table 1 precision testing result
Coefficient of variation CV is generally used for the precision of a measurement assay method, and CV value is less, represents that the result precision of this assay method is better.For clinical chemistry test project, CV be less than 5% method precision generally acknowledge be acceptable.In table 1, the CV value of reagent of the present invention is less than commercial reagent A, shows that the precision of the inventive method is better than commercial reagent A.
2) linear determination: adopt reagent of the present invention and commercial reagent A respectively, adopt Olympus 5400 automatic clinical chemistry analyzer, to 50 parts of urine specimens (comprising normal and exceptional sample), measure by each autoregressive parameter, and correlation analysis is carried out to measured value (the results are shown in Figure 1, the measured value of what X-axis represented is reagent of the present invention, the measured value of what Y-axis represented is commercial reagent A).Related coefficient: r
2=0.9960, linear equation is: y=1.005x+0.041, and result shows that this reagent and commercial reagent correlativity are good.
Table 2 linear correlation detection result
3) Stability Determination: detection kit of the present invention is placed on respectively room temperature and 4 DEG C of refrigerators, substitutes sample with freshly prepared 15mg/L albumin standard, measured 1 time every 1 month, and aggegation required time appears in record, the results are shown in Table 3.Result shows, kit is placed at 4 DEG C of refrigerators and do not had obvious loss of activity in more than at least 7 months, but should not deposit in room temperature.
Table 3 Detection of Stability result
| Under room temperature | There is the time (min) of aggegation | At 4 DEG C | There is the time (min) of aggegation |
[0049]
| New preparation | 1 | New preparation | 1 |
| Deposit 1 month | 2 | Deposit 1 month | 1 |
| Deposit 2 months | Not aggegation | Deposit 2 months | 1 |
| Deposit 3 months | Not aggegation | Deposit 3 months | 1 |
| Deposit 4 months | Not aggegation | Deposit 4 months | 1 |
| Deposit 5 months | Not aggegation | Deposit 5 months | 1 |
| Deposit 6 months | Not aggegation | Deposit 6 months | 1 |
| Deposit 7 months | Not aggegation | Deposit 7 months | 1 |
4) specific assay: choose 4 kinds and disturb albumen and glucose to carry out interference experiment mensuration, equal unrestraint effect, show that this kit has good specificity, result is as shown in table 4:
Table 4 specific detection result
Claims (5)
1. the invention provides a kind of microdose urine protein (mAlb) detection kit, described kit is based on latex enhancing immune turbidimetry, for liquid double reagent, comprise reagent R1 and R2, described reagent R1 is reactant, reagent R2 is the solution containing albumin immunity latex particle, its feature is that applied chemistry method is cross-linked, sheep anti-human albumin antibodies (also claiming goat-anti people mAlb antibody) and Carboxylated Polystyrene latex covalent cross-linking is made, preparation mAlb antibody latex reagent by water-soluble carbodiimide (EDC) and N-hydroxy-succinamide (NHS).
2. Chemical Crosslinking Methods prepares mAlb antibody latex reagent according to claim 1, it is characterized in that Carboxylated Polystyrene latex particle size used is 200nm.
3. Chemical Crosslinking Methods prepares mAlb antibody latex reagent according to claim 1, it is characterized in that damping fluid used is respectively 0.01mol/L, PH7.2 phosphate buffer (PBS), 0.01mol/L, PH7.6 phosphate buffer (PBS).
4. Chemical Crosslinking Methods prepares mAlb antibody latex reagent according to claim 1, it is characterized in that adding water-soluble carbodiimide (EDC) and N-hydroxy-succinamide (NHS) in Carboxylated Polystyrene latex and goat-anti people mAlb antibody covalent cross-linking process respectively.
5. microdose urine protein detection kit is characterized in that a described liquid double reagent, comprises reagent R1 and R2, and both compositions are as follows:
。
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105548560A (en) * | 2015-12-18 | 2016-05-04 | 宁波普瑞柏生物技术有限公司 | A serum albumin detecting reagent and a serum albumin detecting method |
| CN105675887A (en) * | 2016-04-13 | 2016-06-15 | 柏荣诊断产品（上海）有限公司 | High-performance human urine microalbumin detection kit |
| CN106053368A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining microalbuminuria and preparation method thereof |
| CN106093433A (en) * | 2016-06-17 | 2016-11-09 | 上海执诚生物科技有限公司 | Microalbumin test kit based on latex immunoturbidimetry and preparation method thereof |
| CN106872718A (en) * | 2017-04-26 | 2017-06-20 | 吉林省富生医疗器械有限公司 | A kind of microdose urine protein detection kit and preparation method thereof |
| CN106885906A (en) * | 2015-12-16 | 2017-06-23 | 山东博科生物产业有限公司 | A kind of stabilization, the microdose urine protein reagent of strong antijamming capability and detection method |
| CN107102150A (en) * | 2017-05-04 | 2017-08-29 | 上海奥普生物医药有限公司 | A kind of microdose urine protein determines kit and preparation method thereof |
| CN109946295A (en) * | 2017-12-20 | 2019-06-28 | 济南腾奔生物技术有限公司 | A kind of microdose urine protein Immunity transmission turbidity detection kit |
| CN114414508A (en) * | 2021-12-30 | 2022-04-29 | 苏州百源基因技术有限公司 | Urine microalbumin detection kit and use method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1028312A1 (en) * | 1999-02-10 | 2000-08-16 | UMM Electronics, Inc. | Optical analysis device |
| EP1674871A1 (en) * | 2004-12-21 | 2006-06-28 | Ortho-Clinical Diagnostics, Inc. | Methods for improving consistency in assays through additional motion |
| CN102253217A (en) * | 2011-04-07 | 2011-11-23 | 武汉生之源生物科技有限公司 | Detection kit of latex particle enhanced neutrophil gelatinase-associated lipid transfer protein |
| CN102645537A (en) * | 2012-04-26 | 2012-08-22 | 北京美康生物技术研究中心 | Latex enhanced turbidimetric immunoassay kit for diagnosing gastric diseases or gastric cancer, preparation method thereof and application |
| CN102680700A (en) * | 2012-04-27 | 2012-09-19 | 嘉兴九七九生物技术有限公司 | Quantitative testing reagent for liquid microalbumin in urine and method |
| CN103604931A (en) * | 2013-11-15 | 2014-02-26 | 陆上苏 | Human S100 protein detection reagent and preparation method thereof |
-
2015
- 2015-05-02 CN CN201510227239.7A patent/CN104849473A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1028312A1 (en) * | 1999-02-10 | 2000-08-16 | UMM Electronics, Inc. | Optical analysis device |
| EP1674871A1 (en) * | 2004-12-21 | 2006-06-28 | Ortho-Clinical Diagnostics, Inc. | Methods for improving consistency in assays through additional motion |
| CN102253217A (en) * | 2011-04-07 | 2011-11-23 | 武汉生之源生物科技有限公司 | Detection kit of latex particle enhanced neutrophil gelatinase-associated lipid transfer protein |
| CN102645537A (en) * | 2012-04-26 | 2012-08-22 | 北京美康生物技术研究中心 | Latex enhanced turbidimetric immunoassay kit for diagnosing gastric diseases or gastric cancer, preparation method thereof and application |
| CN102680700A (en) * | 2012-04-27 | 2012-09-19 | 嘉兴九七九生物技术有限公司 | Quantitative testing reagent for liquid microalbumin in urine and method |
| CN103604931A (en) * | 2013-11-15 | 2014-02-26 | 陆上苏 | Human S100 protein detection reagent and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| IAN M. BARLOW 等: "Immunoturbldlmetrlc MIcro-AlbumIn Kit Adapted to the Technicon RA-1000", 《CLINICAL CHEMISTRY》 * |
| 储迅涛 等: "免疫胶乳凝集试验测定尿微量 白蛋 白", 《江西医学检验》 * |
| 胡汉宁: "自制尿微量白蛋 白试剂盒在生化分析仪上的临床应用", 《陕西医学检验》 * |
| 金鑫: "老年高血压患者认知功能与微量白蛋白尿的关系", 《中国优秀硕士学位论文全文数据库（医药卫生科技辑）》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106885906A (en) * | 2015-12-16 | 2017-06-23 | 山东博科生物产业有限公司 | A kind of stabilization, the microdose urine protein reagent of strong antijamming capability and detection method |
| CN105548560A (en) * | 2015-12-18 | 2016-05-04 | 宁波普瑞柏生物技术有限公司 | A serum albumin detecting reagent and a serum albumin detecting method |
| CN105675887A (en) * | 2016-04-13 | 2016-06-15 | 柏荣诊断产品（上海）有限公司 | High-performance human urine microalbumin detection kit |
| CN106093433A (en) * | 2016-06-17 | 2016-11-09 | 上海执诚生物科技有限公司 | Microalbumin test kit based on latex immunoturbidimetry and preparation method thereof |
| CN106093433B (en) * | 2016-06-17 | 2018-05-08 | 上海执诚生物科技有限公司 | Microalbumin kit based on latex immunoturbidimetry and preparation method thereof |
| CN106053368A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining microalbuminuria and preparation method thereof |
| CN106872718A (en) * | 2017-04-26 | 2017-06-20 | 吉林省富生医疗器械有限公司 | A kind of microdose urine protein detection kit and preparation method thereof |
| CN107102150A (en) * | 2017-05-04 | 2017-08-29 | 上海奥普生物医药有限公司 | A kind of microdose urine protein determines kit and preparation method thereof |
| CN109946295A (en) * | 2017-12-20 | 2019-06-28 | 济南腾奔生物技术有限公司 | A kind of microdose urine protein Immunity transmission turbidity detection kit |
| CN114414508A (en) * | 2021-12-30 | 2022-04-29 | 苏州百源基因技术有限公司 | Urine microalbumin detection kit and use method thereof |
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Application publication date: 20150819 |