CN104849399A - Dog urinary stone chemical component analysis method and chemical component analysis equipment - Google Patents
Dog urinary stone chemical component analysis method and chemical component analysis equipment Download PDFInfo
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- CN104849399A CN104849399A CN201510280744.8A CN201510280744A CN104849399A CN 104849399 A CN104849399 A CN 104849399A CN 201510280744 A CN201510280744 A CN 201510280744A CN 104849399 A CN104849399 A CN 104849399A
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Abstract
The invention relates to the field of dog urinary stone detection analysis and discloses a dog urinary stone chemical component analysis method and chemical component analysis equipment. A dog urinary stone sample is acquired, cleaned, dried and ground; then the dog urinary stone sample is equally divided into seven parts which are contained in different reaction test tubes; different reaction test tubes are added with different chemical reaction reagents in a dropping manner, and oscillating mixing is carried out; and the chemical composition of the dog urinary stone sample can be judged according to the reaction phenomenon in the reaction test tube after a preset time period. According to the dog urinary stone chemical component analysis method and the chemical component analysis equipment, qualitative chemical analysis automation is realized, the operation is simple and rapid, special complex instrument is not required, the method and the equipment have important significance to clinical treatment and prevention, and the cost is low.
Description
Technical field
The present invention relates to canine urinary calculi and detect analysis field, particularly relate to a kind of chemical composition analysis method and apparatus of canine urinary calculi.
Background technology
Urolithiasis is that the paracrystalline coagulum of inorganic or organic salt in urinary tract stimulates urinary system mucous membrane and a kind of disease of hemorrhage, the inflammation that causes and obstruction, is a kind of common frdquently encountered disease of animal and human's urinary system.Add up according to domestic and international data, the incidence of disease of the dog cat urolithiasis in animal is 0.5-1.0%, and after treatment, the recurrence rate of urolithiasis is also many up to 50-70% in two years.Therefore, correct anti-disease of effecting a permanent cure is necessary, and this analyzes the constituent of urinary calculus with regard to requiring, selects different control prece accordingly.Canine cat urinary calculi generally has following several: calcium oxalate, ammonium magnesium phosphate, uric acid, calcium carbonate, silicate, calcium phosphate, cystine stone etc., wherein, former 4 kinds the most common, account for more than 95% of calculus generation total amount.
The chemical composition of accurate detection urinary calculus, for the correct diagnosis urinary calculus origin cause of formation, prevention urinary calculus is formed and delays its recurrence has important clinical meaning.The main method that current stone composition detects has the methods such as chemical analysis, FFIR (FTIR), Raman spectrum (RS), X-ray diffraction analysis method (XRD) and differential thermal-thermogravimetric.Method in addition for urinary calculus pattern and structure analysis has optical microscope, polarizing microscope, scanning electron microscope (SEM), transmission electron microscope (TEM) and atomic force microscope (AFM) etc.These analytical technologies need high-end equipment mostly, and the personnel of specialty and more sample pre-treatments requirement, be thus used in scientific research, and veterinary clinic practice is not yet applied.
Summary of the invention
The invention provides a kind of chemical composition analysis method of canine urinary calculi, solve in prior art that the chemical composition cost detecting urinary calculus is higher, the technical matters of complicated operation.
The object of the invention is to be achieved through the following technical solutions:
A chemical composition analysis method for canine urinary calculi, comprising:
Obtain canine urinary calculi sample, described canine urinary calculi sample is cleaned, dried and pulverize operation;
Described canine urinary calculi sample standard deviation is divided into seven deciles, puts into differential responses test tube;
To differential responses test tube, instillation different chemical reaction reagent, and carry out vibration married operation;
After Preset Time, according to the reacting phenomenon in reaction tube, judge the chemical composition of described canine urinary calculi sample.
A kind of chemical composition analysis equipment of canine urinary calculi, comprise frame 1, testing table 2, seal closure 3, test tube group 4, dropper group 5, Electromechanical Control module 6, heating module 7 and oscillation module 8, described test tube group 4 is corresponding with described dropper group 5 to be arranged, and being placed on described testing table 2, described seal closure 3 is inner; Described Electromechanical Control module 6 controls described dropper group 5, described heating module 7 and described oscillation module 8 according to experiment flow.
By the chemical composition analysis method and apparatus of a kind of canine urinary calculi provided by the invention, by obtaining canine urinary calculi sample, described canine urinary calculi sample is cleaned, dried and pulverize operation; Described canine urinary calculi sample standard deviation is divided into seven deciles, puts into differential responses test tube; To differential responses test tube, instillation different chemical reaction reagent, and carry out vibration married operation; After Preset Time, according to the reacting phenomenon in reaction tube, judge the chemical composition of described canine urinary calculi sample.Achieve the robotization of qualitative chemical analysis, fast easy and simple to handle, without the need to the instrument and equipment of Special complex, to clinical treatment with prevent significant, and also with low cost.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, also can obtain other accompanying drawing according to these accompanying drawings.
The process flow diagram of the chemical composition analysis method of a kind of canine urinary calculi that Fig. 1 provides for the embodiment of the present invention;
The structural drawing of the chemical composition analysis equipment of a kind of canine urinary calculi that Fig. 2 provides for the embodiment of the present invention.
Embodiment
For enabling above-mentioned purpose of the present invention, feature and advantage become apparent more, and below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
If Fig. 1 is for providing a kind of process flow diagram of chemical composition analysis method of canine urinary calculi in the embodiment of the present invention, comprising:
Step 101, acquisition canine urinary calculi sample, clean described canine urinary calculi sample, dry and pulverize operation;
Step 102, described canine urinary calculi sample standard deviation is divided into seven deciles, puts into differential responses test tube;
Step 103, to differential responses test tube, instillation different chemical reaction reagent, and carry out vibration married operation;
After step 104, Preset Time, according to the reacting phenomenon in reaction tube, judge the chemical composition of described canine urinary calculi sample.
Wherein, step 103 can also comprise:
To in the first reaction tube, instillation hydrochloric acid 1ml;
To in the second reaction tube, instill 20% sodium acetate 0.5ml;
To in the 3rd reaction tube, instillation ammonium molybdate reagent 0.5ml, and carry out heating operation;
To in the 4th reaction tube, instill 10% sodium carbonate 0.4ml, and carry out vibration married operation, then instill phosphotungstate reagents 0.2ml;
To in the 5th reaction tube, instillation hydrochloric acid 1ml, and carry out vibration married operation, then instill NaOH 0.5ml;
To in the 6th reaction tube, instillation hydrochloric acid 1ml, and carry out vibration married operation, then instill NaOH 0.5ml, then instill titan yellow 0.2ml;
To in the 7th reaction tube, instillation hydrochloric acid 1ml, and carry out vibration married operation, then instill NaOH 0.5ml, then instill Nessler's reagent 0.2ml;
Step 104 can also comprise:
After Preset Time, if described first reaction tube is released if any gas, bubble of emerging, then judge that described canine urinary calculi sample contains carbonate;
After Preset Time, if described second reaction tube is if any white precipitate, then judge that described canine urinary calculi sample contains oxalates;
After Preset Time, if described 3rd reaction tube is as occurred yellow or yellow mercury oxide, then judge that described canine urinary calculi sample contains phosphate;
After Preset Time, if described 4th reaction tube is as become blueness, then judge that described canine urinary calculi sample contains uric acid;
After Preset Time, if described 5th reaction tube is as occurred white precipitate, then judge that described canine urinary calculi sample contains calcium salt;
After Preset Time, if described 6th reaction tube is as occurred blood red precipitation, then judge that described canine urinary calculi sample contains magnesium salts;
After Preset Time, if described 7th reaction tube as displaing yellow or occur red-brown precipitation, then judges that described canine urinary calculi sample contains ammonium.
In the embodiment of the present invention, finally also need to utilize computing machine, testing result is analyzed, finally to determine the chemical composition of canine urinary calculi, namely be whether positive according to detection ion, judge that its calculus forms, such as: in calculus sample, phosphate, ammonium, magnesium are all positive, are namely judged to be struvite calculus; Oxalates, calcium are all positive, are namely judged to be calcinm oxalate calculus.In general, stone composition is all more single, but also there is a small amount of combination calculus existence, such as, when phosphate and calcium are all positive, just may have the existence of calcium phosphate composition.
By the chemical composition analysis method of a kind of canine urinary calculi provided by the invention, by obtaining canine urinary calculi sample, described canine urinary calculi sample is cleaned, dried and pulverize operation; Described canine urinary calculi sample standard deviation is divided into seven deciles, puts into differential responses test tube; To differential responses test tube, instillation different chemical reaction reagent, and carry out vibration married operation; After Preset Time, according to the reacting phenomenon in reaction tube, judge the chemical composition of described canine urinary calculi sample.Achieve the robotization of qualitative chemical analysis, fast easy and simple to handle, without the need to the instrument and equipment of Special complex, to clinical treatment with prevent significant, and also with low cost.
The embodiment of the present invention additionally provides a kind of chemical composition analysis equipment of canine urinary calculi, as shown in Figure 2, comprise frame 1, testing table 2, seal closure 3, test tube group 4, dropper group 5, Electromechanical Control module 6, heating module 7 and oscillation module 8, described test tube group 4 is corresponding with described dropper group 5 to be arranged, and being placed on described testing table 2, described seal closure 3 is inner; Described Electromechanical Control module 6 controls described dropper group 5, described heating module 7 and described oscillation module 8 according to experiment flow.
Through the above description of the embodiments, those skilled in the art can be well understood to the mode that the present invention can add required hardware platform by software and realize, can certainly all be implemented by hardware, but in a lot of situation, the former is better embodiment.Based on such understanding, what technical scheme of the present invention contributed to background technology can embody with the form of software product in whole or in part, this computer software product can be stored in storage medium, as ROM/RAM, magnetic disc, CD etc., comprising some instructions in order to make a computer equipment (can be personal computer, server, or the network equipment etc.) perform the method described in some part of each embodiment of the present invention or embodiment.
Above to invention has been detailed introduction, applying specific case herein and setting forth principle of the present invention and embodiment, the explanation of above embodiment just understands method of the present invention and core concept thereof for helping; Meanwhile, for one of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, in sum, this description should not be construed as limitation of the present invention.
Claims (4)
1. a chemical composition analysis method for canine urinary calculi, is characterized in that, comprising:
Obtain canine urinary calculi sample, described canine urinary calculi sample is cleaned, dried and pulverize operation;
Described canine urinary calculi sample standard deviation is divided into seven deciles, puts into differential responses test tube;
To differential responses test tube, instillation different chemical reaction reagent, and carry out vibration married operation;
After Preset Time, according to the reacting phenomenon in reaction tube, judge the chemical composition of described canine urinary calculi sample.
2. the chemical composition analysis method of canine urinary calculi according to claim 1, is characterized in that, described to differential responses test tube, instillation different chemical reaction reagent, and carries out vibration married operation, comprising:
To in the first reaction tube, instillation hydrochloric acid 1ml;
To in the second reaction tube, instill 20% sodium acetate 0.5ml;
To in the 3rd reaction tube, instillation ammonium molybdate reagent 0.5ml, and carry out heating operation;
To in the 4th reaction tube, instill 10% sodium carbonate 0.4ml, and carry out vibration married operation, then instill phosphotungstate reagents 0.2ml;
To in the 5th reaction tube, instillation hydrochloric acid 1ml, and carry out vibration married operation, then instill NaOH 0.5ml;
To in the 6th reaction tube, instillation hydrochloric acid 1ml, and carry out vibration married operation, then instill NaOH 0.5ml, then instill titan yellow 0.2ml;
To in the 7th reaction tube, instillation hydrochloric acid 1ml, and carry out vibration married operation, then instill NaOH 0.5ml, then instill Nessler's reagent 0.2ml.
3. the chemical composition analysis method of canine urinary calculi according to claim 2, is characterized in that, after described Preset Time, according to the reacting phenomenon in reaction tube, judges the chemical composition of described canine urinary calculi sample, comprising:
After Preset Time, if described first reaction tube is released if any gas, bubble of emerging, then judge that described canine urinary calculi sample contains carbonate;
After Preset Time, if described second reaction tube is if any white precipitate, then judge that described canine urinary calculi sample contains oxalates;
After Preset Time, if described 3rd reaction tube is as occurred yellow or yellow mercury oxide, then judge that described canine urinary calculi sample contains phosphate;
After Preset Time, if described 4th reaction tube is as become blueness, then judge that described canine urinary calculi sample contains uric acid;
After Preset Time, if described 5th reaction tube is as occurred white precipitate, then judge that described canine urinary calculi sample contains calcium salt;
After Preset Time, if described 6th reaction tube is as occurred blood red precipitation, then judge that described canine urinary calculi sample contains magnesium salts;
After Preset Time, if described 7th reaction tube as displaing yellow or occur red-brown precipitation, then judges that described canine urinary calculi sample contains ammonium.
4. the chemical composition analysis equipment of a canine urinary calculi, it is characterized in that, comprise frame (1), testing table (2), seal closure (3), test tube group (4), dropper group (5), Electromechanical Control module (6), heating module (7) and oscillation module (8), described test tube group (4) is corresponding with described dropper group (5) to be arranged, and being placed on described testing table (2), described seal closure (3) is inner; Described Electromechanical Control module (6) controls described dropper group (5), described heating module (7) and described oscillation module (8) according to experiment flow.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105954257A (en) * | 2016-05-24 | 2016-09-21 | 厦门市卓厦光电科技有限公司 | Intelligent human body calculus component analyzer and application method thereof |
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Application publication date: 20150819 |