CN104846047A - Method for preparing zein antihypertensive peptide based on ultrasonic reverberant field - Google Patents
Method for preparing zein antihypertensive peptide based on ultrasonic reverberant field Download PDFInfo
- Publication number
- CN104846047A CN104846047A CN201510052239.8A CN201510052239A CN104846047A CN 104846047 A CN104846047 A CN 104846047A CN 201510052239 A CN201510052239 A CN 201510052239A CN 104846047 A CN104846047 A CN 104846047A
- Authority
- CN
- China
- Prior art keywords
- ultrasonic
- liquid
- zein
- khz
- ultrasonic wave
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Seasonings (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for preparing zein antihypertensive peptide based on an ultrasonic reverberant field, which belongs to the technical field of deep processing of agricultural and sideline products. The extract of the zein antihypertensive peptide can be prepared by preparing 180 mL of zein suspension with a concentration of 45 g/L, subjecting the zein suspension to ultrasonic pretreatment in an ultrasonic solid-liquid reverberant field, adding alkali protease for enzymatic hydrolysis after pretreatment and collecting supernatant after completion of enzymatic hydrolysis. Under the above-mentioned conditions, pretreated zein enzymatic hydrolysate has ACE and DH inhibitory activity of 42.93% and 10.35%, respectively, an increase by 44.9% and 37.6% respectively compared with untreated zein enzymatic hydrolysate. According to the invention, the zein suspension is pretreated by solid-liquid sweep-frequency ultrasonic waves, so enzymatic hydrolysis efficiency is improved, and enzymatic hydrolysis effects are enhanced.
Description
Technical field
The present invention relates to bioengineering field and corn deep processing field, refer in particular to and utilize frequency sweep ultrasonic ripple pre-treatment zein raw material in solid-liquid ultrasonic wave reverberation field, recycling enzyme solution prepares a kind of technology of zein ace inhibitory peptide.
Technical background
Corn is one of world's Three major grain crops, the farm crop that Ye Shi China is traditional, and in China, plantation is with a long history, and China's corn yield occupies second place of the world, and annual production accounts for 25% of the world.Zein powder contains rich in protein, also containing 15 kinds of inorganic salt and the exclusive yellow pigment of corn, but the protein contained by it lacks Methionin, the essential amino acids such as tryptophane, and finished product has special taste and color and luster, therefore domesticly mainly use it for roughage preparation, make nutritive food additive, or directly process discharge, greatly waste resource.In recent years, the research that protein involved is hydrolyzed into bioactive peptide is more, and the report wherein preparing corn bioactive peptide through protease hydrolysis about zein is also a lot.At present, a large amount of ace inhibitory peptides has been found from the enzymolysis product of zein, usually be also referred to as blood pressure lowering peptide, test shows that these ace inhibitory peptides can show good hypotensive effect to spontaneously hypertensive patient, to normotensive then without hypotensive effect.
But zein contains a large amount of hydrophobic groupings, and solvability is poor, there will be the not accessible restriction enzyme site of proteolytic enzyme in enzymolysis process, hydrolysis rate is slow, the low conversion rate of enzymolysis efficiency and product, the problems such as unstable product quality.The functional property of protein is primarily of its structures shape, and ultrasonic wave can make the state of medium, composition, structure and function etc. change, and more restriction enzyme sites come out, and increases the contact frequency of substrate and enzyme, to strengthen hydrolysis result.But ultrasonic reverberation field and ultrasound works pattern directly have influence on the intensity of variation of protein structure, improvement and the bioactive raising of this change and its enzymolysis property are closely related.So-called reverberation field is the ultrasonic wave of 20 kHz ~ 50 kHz exactly for frequency, absorption in the liquid medium of the aqueous solution one class is little, it will experience the multiple reflections at container inner wall, liquid level in phonochemical reaction device, although the synthesis sound field of the identical superposition but the sound wave of the random change of phase place meets at space point of these frequencies, its total sound intensity equals each row intensity of acoustic wave sum, namely do not interfere between them, this sound field is called reverberation field.It may be the principal mode of sound field in phonochemical reaction device.
In existing bibliographical information, most research is all adopt single-frequency and be that the ultrasonic device of fixed frequency processes protein, the demand of material composition change can not be met, namely ultrasonic wave can not produce with the natural frequency of raw material and farthest resonate, the due action effect of ultrasonic wave can not be given full play to, and rarely have both at home and abroad at present the research report of different mode ultrasonic pretreatment on zein physico-chemical property, constitutional features, enzymolysis property impact.
Summary of the invention
The object of the invention is to adopt frequency sweep ultrasonic ripple reverberation field pre-treatment zein, be intended to the activity improving enzymolysis product, improve hydrolysis result, reduce energy consumption.
Above-mentioned purpose of the present invention is realized by following technique means:
First design ultrasonic wave reverberation field, then ultrasonic pretreatment is carried out to corn protein solution, finally carry out enzyme digestion reaction.Concrete technical scheme is, a kind of method preparing zein blood pressure lowering peptide based on ultrasonic wave reverberation field: carry out according to following step:
(1) reverberation field is designed: namely reverberation field is the ultrasonic wave of 20 kHz ~ 50kHz for frequency, absorption in the liquid medium of the aqueous solution one class is little, it will experience the multiple reflections at container inner wall, liquid level in phonochemical reaction device, although the synthesis sound field of the identical superposition but the sound wave of the random change of phase place meets at space point of these frequencies, its total sound intensity equals each row intensity of acoustic wave sum, does not namely interfere between them.Learn according to definition, when different interface exists, can reflect, and form reverberation field.
The present invention designs solid-liquid, liquid-liquid, solution-air three kinds of interfaces respectively, form the ultrasonic reverberation field of different shape, solid-liquid interface idle ultrasonic flat board and water body are formed, and liquid-liquid interface vegetables oil and water body are formed, and liquid-vapo(u)r interface air and water body are formed.
(2) protein raw materials is taken, the aqueous solution is made into according to material concentration 4.5%, carry out the pre-treatment of solid-liquid frequency sweep ultrasonic, treatment condition are: working hour 10 s of initial temperature 18 DEG C, impulse ultrasound and intermittent time 3 s, ultrasonic frequency sweep cycle 500 ms, sonication treatment time 30 min, unit volume ultrasonic power 120 W/L.Swept frequency is 22 ± 2 kHz, 28 ± 2 kHz, 33 ± 2 kHz, 40 ± 2 kHz, 68 ± 2 kHz.
(3) Sumizyme MP enzymolysis after pre-treatment: reaction conditions is: concentration of substrate 4.5%, enzyme concentration 5616U/g, enzymolysis time 60 min, hydrolysis temperature 50 DEG C, enzymolysis pH 9.0.After enzymolysis terminates, 0.2 mol/L HCL is used to regulate pH to 7.0, and the enzyme 10min that goes out in boiling water bath, in the centrifugal 10min of 12000 rpm after cooling, collect supernatant liquor, the extracting solution of protein blood pressure-reducing peptide can be obtained.
the present invention advantageously
The present invention adopts solid-liquid frequency sweep ultrasonic ripple reverberation field pre-treatment Zein powder suspension, and be studied the ACE inhibitory activity of enzymolysis product after ultrasonication, the ACE inhibiting rate activity of enzymolysis solution improves 44.9%, DH and improves 37.6%.Compared with the ultrasonic pretreatment mode of other classifications, improve the activity of enzymolysis product, find in the ultrasonic reverberation field formed at liquid-liquid interface, very easily there is significant emulsion, so cannot form this type of interface in test and actual production, this has certain values for instrument and equipment design and industrial production.
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Accompanying drawing explanation
Fig. 1 is ultrasonic response liquid-liquid transmission medium;
Fig. 2 is ultrasonic response solution-air transmission medium;
Fig. 3 is ultrasonic response solid-liquid transmission medium.
Embodiment
Weigh the reaction efficiency of whole reaction in the present invention with degree of hydrolysis (DH), the mensuration of degree of hydrolysis (DH) adopts pH-stat method.In human body, too much angiotensin-converting enzyme (ACE) is one of major reason caused elevation of the blood pressure simultaneously, and therefore the present invention evaluates its hypotensive activity with the inhibiting rate of enzymolysis product to ACE.The mensuration of enzymolysis product ACE inhibitory activity is according to document " Liu Zhiguo, Wu Qiong, Lv Lingxiao etc.Enzymolysis rice bran protein separation and Extraction ace inhibitory peptide and structural research [J] thereof. Food science, 2007,28 (3): 223 ~ 227. " method is carried out; using N-[3-(2-furyl) acryloyl]-L-phenylalanyl glycylglycine (FAPGG) as the substrate of ACE catalysis; study the change of its absorbancy, utilize enzyme scalar quantity instrument to measure.
The present invention designs reverberation field: namely reverberation field is the ultrasonic wave of 20 kHz ~ 50kHz for frequency, absorption in the liquid medium of the aqueous solution one class is little, it will experience the multiple reflections at container inner wall, liquid level in phonochemical reaction device, although the synthesis sound field of the identical superposition but the sound wave of the random change of phase place meets at space point of these frequencies, its total sound intensity equals each row intensity of acoustic wave sum, does not namely interfere between them.Learn according to definition, when different interface exists, can reflect, and form reverberation field.
The present invention designs solid-liquid, liquid-liquid, solution-air three kinds of interfaces respectively, form the ultrasonic reverberation field of different shape, solid-liquid interface idle ultrasonic flat board and water body are formed, and liquid-liquid interface vegetables oil and water body are formed, and liquid-vapo(u)r interface air and water body are formed.
Fig. 1 is the liquid-liquid transmission medium ultrasonic wave reverberation field used in the present invention.1 is ultrasonic reaction pond, and 2 is oil phase (being formed by vegetables oil), and 4 is aqueous phase (being formed by distilled water), and the contact surface 2 and 4 forms liquid-liquid transmission interface.5 is ultrasonic wave generation plate, and can send the ultrasonic wave of 22 ± 2 kHz, 28 ± 2 kHz, 33 ± 2 kHz, 40 ± 2 kHz, 68 ± 2 kHz frequencies, ultrasound mode is frequency sweep ultrasonic, and power is 600 W.3 is sample sack, is placed in the region intermediate of 2 and 4, in liquid-liquid ultrasonic wave reverberation field, carry out ultrasonic pretreatment.
Fig. 2 is the solution-air transmission medium ultrasonic wave reverberation field used in the present invention.1 is ultrasonic reaction pond, and 2 is gas phase (being formed by air), and 4 is aqueous phase (being formed by distilled water), and the contact surface 2 and 4 forms solution-air transmission interface.5 is ultrasonic wave generation plate, and can send the ultrasonic wave of 22 ± 2 kHz, 28 ± 2 kHz, 33 ± 2 kHz, 40 ± 2 kHz, 68 ± 2 kHz frequencies, ultrasound mode is frequency sweep ultrasonic, and power is 600 W.3 is sample sack, is placed in the region intermediate of 2 and 4, in solution-air ultrasonic wave reverberation field, carry out ultrasonic pretreatment.
Fig. 3 is the solid-liquid transmission medium ultrasonic wave reverberation field used in the present invention.1 is ultrasonic reaction pond, and 2 is solid phase (being formed by idle ultrasonic flat board), and 4 is aqueous phase (being formed by distilled water), and the contact surface 2 and 4 forms solid-liquid transmission interface.5 is ultrasonic wave generation plate, and can send the ultrasonic wave of 22 ± 2 kHz, 28 ± 2 kHz, 33 ± 2 kHz, 40 ± 2 kHz, 68 ± 2 kHz frequencies, ultrasound mode is frequency sweep ultrasonic, and power is 600 W.3 is sample sack, is placed in the region intermediate of 2 and 4, in solid-liquid ultrasonic wave reverberation field, carry out ultrasonic pretreatment.
reference examples:conventional enzymolysis: compound concentration is zein suspension 180 mL of 45 g/L, be stir 30 min under the condition of 18 DEG C in temperature, add Sumizyme MP and carry out enzyme digestion reaction, reaction conditions is: enzyme concentration 5616 U/g([E]/[S]), enzymolysis time 60 min, hydrolysis temperature 50 DEG C, enzymolysis pH 9.0.After enzymolysis terminates, 0.2 mol/L HCL is used to regulate pH to 7.0, and enzyme 10 min that goes out in boiling water bath, in centrifugal 10 min of 12000 rpm after cooling, get supernatant liquor and be settled to 800 mL, redilution 5 times, measure ACE inhibit activities.Adopt pH-stat method control pH in process of the test, and measure DH value.Parallel three tests, through measuring, the ACE inhibiting rate of zein enzymolysis product diluent is 19.81%.DH is 7.52%.
embodiment 1:the ultrasonic reverberation field of design solid-liquid transmission medium, solid-liquid interface idle ultrasonic flat board and water body are formed.Compound concentration is zein suspension 180 mL of 45g/L, carry out ultrasonic pretreatment, ultrasound works pattern is frequency sweep mode (40 ± 2) kHz, and treatment condition are: working hour 10 s of initial temperature 18 DEG C, impulse ultrasound and intermittent time 3 s, ultrasonic frequency sweep cycle 500 ms, sonication treatment time 30 min, unit volume ultrasonic power 120 W/L.Feed liquid Sumizyme MP after supersound process is directly hydrolyzed reaction, and reaction conditions is: concentration of substrate 4.5%, enzyme concentration 5616 U/g([E]/[S]), enzymolysis time 60 min, hydrolysis temperature 50 DEG C, enzymolysis pH 9.0.After enzymolysis terminates, 0.2 mol/L HCL is used to regulate pH to 7.0, and enzyme 10 min that goes out in boiling water bath, in centrifugal 10 min of 12000 rpm after cooling, get supernatant liquor and be settled to 800 mL, redilution 5 times, measure ACE inhibit activities.The ACE inhibiting rate of enzymolysis supernatant liquor is 25.68%, DH is 8.81%.Reference examples is compared, and ACE inhibiting rate improves 29.63%, DH and improves 15.8%.
embodiment 2:the ultrasonic reverberation field of design solution-air transmission medium, liquid-vapo(u)r interface air and water body are formed.Compound concentration is zein suspension 180 mL of 45g/L, carries out ultrasonic pretreatment, ultrasonic procedure and the same example of enzymolysis process (1).The ACE inhibiting rate of enzymolysis supernatant liquor is 23.96%, DH is 9.24%.Reference examples is compared, and ACE inhibiting rate improves 20.94%, DH and improves 21.4%.
embodiment 3:the ultrasonic reverberation field of design liquid-liquid transmission medium, liquid-liquid interface oil and water body are formed.Compound concentration is zein suspension 180 mL of 45g/L, carries out ultrasonic pretreatment, and the ACE inhibiting rate of ultrasonic procedure and the same example of enzymolysis process (1) enzymolysis supernatant liquor is 16.03%, DH is 7.41%.Compared with reference examples, ACE inhibiting rate have dropped 19.08%, DH and have dropped 2.6%.In the ultrasonic reverberation field that liquid-liquid interface is formed, significant emulsion very easily occurs, so should avoid the formation of this type of interface in test and actual production, this has certain reference value for instrument and equipment design and industrial production.
embodiment 4:compound concentration is zein suspension 180 mL of 45 g/L, ultrasonic 30 min in solid-liquid frequency sweep ultrasonic ripple reverberation field.Ultrasonic frequency is 22 ± 2 kHz, initial temperature 18 DEG C, working hour 10 s of impulse ultrasound, intermittent time 3 s, frequency sweep cycle 500 ms, unit volume ultrasonic power 120 W/L.Add Sumizyme MP and carry out enzyme digestion reaction, the same reference examples of reaction conditions.After enzymolysis terminates, 0.2 mol/L HCL is used to regulate pH to 7.0, and enzyme 10 min that goes out in boiling water bath, in the centrifugal 10min of 12000 rpm after cooling, get supernatant liquor and be settled to 800 mL, redilution 5 times, measure ACE inhibit activities.Adopt pH-stat method control pH in process of the test, and measure DH value.Parallel three tests.Through measuring, the ACE inhibiting rate of zein enzymolysis product diluent is 41.38%, DH is 10.22%.
embodiment 5:experimentation is with example 4, and it is not all swept frequency is 28 ± 2 kHz.Through measuring, the ACE inhibiting rate of zein enzymolysis product diluent is 22.25%.DH is 10.15%.
embodiment 6:experimentation is with example 4, and it is not all swept frequency is 33 ± 2 kHz.Through measuring, the ACE inhibiting rate of zein enzymolysis product diluent is 29.94%.DH is 9.91%.
embodiment 7:experimentation is with example 4, and it is not all swept frequency is 40 ± 2 kHz.Through measuring, the ACE inhibiting rate of zein enzymolysis product diluent is 42.93%.DH is 10.35%.
embodiment 8:experimentation is with example 4, and it is not all swept frequency is 68 ± 2 kHz.Through measuring, the ACE inhibiting rate of zein enzymolysis product diluent is 32.24%.DH is 9.87%.
Claims (5)
1. prepare a method for zein blood pressure lowering peptide based on ultrasonic wave reverberation field, it is characterized in that carrying out according to following step:
(1) take protein raw materials, be made into the aqueous solution according to material concentration 4.5%, carry out ultrasonic wave reverberation field ultrasonic pretreatment,
(2) Sumizyme MP enzymolysis after pre-treatment: reaction conditions is: concentration of substrate 4.5%, enzyme concentration 5616U/g, enzymolysis time 60 min, hydrolysis temperature 50 DEG C, enzymolysis pH 9.0; After enzymolysis terminates, 0.2 mol/L HCL is used to regulate pH to 7.0, and the enzyme 10min that goes out in boiling water bath, in the centrifugal 10min of 12000 rpm after cooling, collect supernatant liquor, the extracting solution of protein blood pressure-reducing peptide can be obtained.
2. a kind of method preparing zein blood pressure lowering peptide based on ultrasonic wave reverberation field according to claim 1, is characterized in that swept frequency is 22 ± 2 kHz, 28 ± 2 kHz, 33 ± 2 kHz, 40 ± 2 kHz or 68 ± 2 kHz.
3. a kind of method preparing zein blood pressure lowering peptide based on ultrasonic wave reverberation field according to claim 1, it is characterized in that ultrasonic wave reverberation field is solid-liquid, liquid-liquid, solution-air three kinds of interfaces, form the ultrasonic reverberation field of different transmission medium, solid-liquid interface idle ultrasonic flat board and water body are formed, liquid-liquid interface vegetables oil and water body are formed, and liquid-vapo(u)r interface air and water body are formed.
4. a kind of method preparing zein blood pressure lowering peptide based on ultrasonic wave reverberation field according to claim 1, it is characterized in that, supersound process condition is: working hour 10 s of initial temperature 18 DEG C, impulse ultrasound and intermittent time 3 s, ultrasonic frequency sweep cycle 500 ms, sonication treatment time 30 min, unit volume ultrasonic power 120 W/L.
5. a kind of method preparing zein blood pressure lowering peptide based on ultrasonic wave reverberation field according to claim 3, is characterized in that the preferred solid-liquid transmission medium of ultrasonic wave reverberation field, and frequency sweep ultrasonic ripple reverberation field frequency is frequently: (40 ± 2) kHz.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510052239.8A CN104846047A (en) | 2015-01-30 | 2015-01-30 | Method for preparing zein antihypertensive peptide based on ultrasonic reverberant field |
| PCT/CN2016/071590 WO2016119629A1 (en) | 2015-01-30 | 2016-01-21 | Method for preparing functional polypeptide through multimode ultrasonic enhancing enzymolysis |
| US15/547,458 US20180023110A1 (en) | 2015-01-30 | 2016-01-21 | Method for preparing functional polypeptide through multimode ultrasonic enhancing enzymolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510052239.8A CN104846047A (en) | 2015-01-30 | 2015-01-30 | Method for preparing zein antihypertensive peptide based on ultrasonic reverberant field |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104846047A true CN104846047A (en) | 2015-08-19 |
Family
ID=53846034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510052239.8A Pending CN104846047A (en) | 2015-01-30 | 2015-01-30 | Method for preparing zein antihypertensive peptide based on ultrasonic reverberant field |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104846047A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016119629A1 (en) * | 2015-01-30 | 2016-08-04 | 江苏大学 | Method for preparing functional polypeptide through multimode ultrasonic enhancing enzymolysis |
| CN106119329A (en) * | 2016-07-08 | 2016-11-16 | 江苏大学 | A kind of synchronize the method that multiple frequency ultrasonic assisted immobilization enzyme enzymolysis prepares Semen Maydis polypeptide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101711969A (en) * | 2009-10-30 | 2010-05-26 | 江苏大学 | Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof |
| CN102398959A (en) * | 2011-11-18 | 2012-04-04 | 江苏大学 | Method for degrading organophosphorus pesticide based on sweep-frequency double-frequency ultrasonic technology |
-
2015
- 2015-01-30 CN CN201510052239.8A patent/CN104846047A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101711969A (en) * | 2009-10-30 | 2010-05-26 | 江苏大学 | Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof |
| CN102398959A (en) * | 2011-11-18 | 2012-04-04 | 江苏大学 | Method for degrading organophosphorus pesticide based on sweep-frequency double-frequency ultrasonic technology |
Non-Patent Citations (3)
| Title |
|---|
| WENJUANQU等: "Enzymolysis kinetics and activities of ACE inhibitory peptides from wheat germ protein prepared with SFP ultrasound-assisted processing", 《ULTRASONICS SONOCHEMISTRY》 * |
| XIAOFENG REN等: "Effects of sweeping frequency ultrasound treatment on enzymatic preparations of ACE-inhibitory peptides from zein", 《EUR FOOD RES TECHNOL》 * |
| 毛舒云: "扫频超声辅助酶法制备玉米ACE抑制肽的研究", 《中国学位论文全文数据库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016119629A1 (en) * | 2015-01-30 | 2016-08-04 | 江苏大学 | Method for preparing functional polypeptide through multimode ultrasonic enhancing enzymolysis |
| CN106119329A (en) * | 2016-07-08 | 2016-11-16 | 江苏大学 | A kind of synchronize the method that multiple frequency ultrasonic assisted immobilization enzyme enzymolysis prepares Semen Maydis polypeptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101711969B (en) | Device and method for biological processing with pulsed multi-frequency ultrasonic waves in sweep-frequency mode and use thereof | |
| US20180023110A1 (en) | Method for preparing functional polypeptide through multimode ultrasonic enhancing enzymolysis | |
| CN106119329A (en) | A kind of synchronize the method that multiple frequency ultrasonic assisted immobilization enzyme enzymolysis prepares Semen Maydis polypeptide | |
| CN101999266B (en) | Method and device for promoting increase in yield by treating rice seeds with sound waves | |
| CN103360509B (en) | Alginic acid and the method for alginic acid salt is prepared for raw material with fresh sargassum | |
| CN104313098A (en) | Method for preparing swimming bladder collagen powder | |
| CN103932267A (en) | Processing technique for preparing oyster meat nutritious powder by enzymolysis with assistance of ultrasound | |
| CN101418329B (en) | Preparation method of rapeseed proteolysis peptides based on pulse ultrasonic technology and use thereof | |
| Ma et al. | Pretreatment of garlic powder using sweep frequency ultrasound and single frequency countercurrent ultrasound: Optimization and comparison for ACE inhibitory activities | |
| CN104846046A (en) | Method for preparing gluten antihypertensive peptide based on sequential ultrasonic enhanced enzymolysis | |
| CN101200751A (en) | Method for preparing hairtail polypeptides | |
| CN104068423A (en) | Oyster meat nutrition powder and preparation method thereof | |
| CN107056381A (en) | A kind of preparation method rich in polypeptide deep-sea fish protein liquid | |
| CN104774894A (en) | Method for preparing gluten ACE inhibitory peptide through multi-mode ultrasonic wave strengthened enzymolysis | |
| CN104846047A (en) | Method for preparing zein antihypertensive peptide based on ultrasonic reverberant field | |
| CN101322541B (en) | Method for preparing delicate flavor agent using fresh water fish leftover bits and pieces | |
| CN107190038A (en) | Microorganism prepares active peptide technique | |
| CN109369774A (en) | The extracting method of protein in a kind of acer truncatum seed dregs of rice | |
| CN105212200A (en) | The extracting method of natural bacterium mushroom element and the fresh condiment preparation method of rich bacterium mushroom element vegetables in edible mushroom | |
| CN101720905B (en) | Flavor yeast albumen powder and preparation method thereof | |
| CN104845959A (en) | Method for preparing rapeseed polypeptide by using ultrasonic-assisted enzymolysis and immobilized alkali protease | |
| CN110229859A (en) | A kind of efficient preparation process of the anti-oxidant calcium ion chelating peptide in Optimization of Low Value Fish visceral protein source | |
| CN106689641A (en) | Production technology of walnut peptide powder | |
| CN102653780A (en) | Method for preparing low molecular peptide through proteolysis of pumpkin seeds and dregs | |
| CN201579050U (en) | Sweep-frequency mode pulse multi-frequency ultrasonic biological processing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150819 |