CN104846036A - Isomaltulose preparation method - Google Patents
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- CN104846036A CN104846036A CN201510297031.2A CN201510297031A CN104846036A CN 104846036 A CN104846036 A CN 104846036A CN 201510297031 A CN201510297031 A CN 201510297031A CN 104846036 A CN104846036 A CN 104846036A
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Abstract
The invention discloses an isomaltulose preparation method which comprises the following steps: (1) by taking a cane sugar solution as a raw material, mixing the cane sugar solution and immobilized thalli containing Alpha-glucosyltransferase in a conversion tank, wherein the addition amount of the immobilized thalli is 5-10 percent of that of the cane sugar solution; introducing sterile compressed air into the conversion tank to perform conversion at 25-32DEG C and the pH of 5-7.5, and ending the conversion when the residual quantity of cane sugar in a conversion solution is below 2 percent; and (2) processing the conversion solution through the procedures of decoloring, filtering, ion-exchange purification, concentration, and cooling crystallization to obtain crystal isomaltulose. The isomaltulose preparation method is simple and convenient to operate, low in equipment investment and production cost and free from potential food security hazards, and can realize industrial production.
Description
Technical field
The present invention relates to a kind of preparation method of Palatinose, be specifically related to a kind of method directly utilizing microbial transformation sugar industry Palatinose, belong to technical field of biological fermentation.
Background technology
Palatinose (Isomaltulose), also known as palatinose, chemical name is α-D-glucopyranosyl-1,6-D-fructose is that a kind of glucose and fructose combine the reducing disaccharides formed with α-1,6-glycosidic link, naturally be present in honey, the isomers of sucrose, its mouthfeel and physical properties and sucrose closely similar, and about sugariness only has the half of sucrose.Because human body itself cannot digest Palatinose, only have and just can enter blood after slowly being decomposed by enteric microorganism and be absorbed by the body, therefore, the speed discharging monose after edible in blood stimulates insulin secretion slowly and not than sucrose, is thus of value to the control of diabetes and can prevents fatty too much accumulation.Due to the physiological function that Palatinose is special, as a kind of heath food additive, foodstuffs industry can be applied to as sucrose substitute under given conditions.
The batch production of Palatinose originates from the middle and later periods eighties 20th century, take sucrose as raw material, at sucrose isomerase (alpha-glucosyl transferring enzyme, EC.5.4.99.11) under effect, α-1, the 2-glycosidic link be connected with fructose by glucose in sucrose molecules conjugates as α-1,6-glycosidic link, form a kind of and the diverse new sugar of sucrose physics, chemical property and physiological function, i.e. Palatinose.At present, the sucrose isomerase produced for Palatinose derives from various microorganism, as klebsiella (Klebsiella), Protaminobacter (Psedumonas), serratia (Serratia), erwinia (Erwinia), Agrobacterium (Agrobacterium), Rhodopseudomonas (Psedumonas) etc.
At present, countries in the world such as Germany, the U.S., Japan, China etc. all adopt immobilized enzyme method to produce Palatinose.The process for preparing isomaltoketose of Chinese patent literature report, take passages as follows:
Chinese patent CN1030530C, preparation of parakin sugar by solidified alpha-glucosyl group transferase, the technique of this disclosure of the invention obtained palatinose by alpha-glucosyl transferring enzyme strain fermentation, immobilization, sucrose inversion and condensing crystal, adopt kaolin to adsorb in its technological process and collect the thalline in fermented liquid, then sodium alginate is adopted to mix with thalline, be added dropwise to being fixed in calcium chloride, then obtain the immobilized enzyme for invert sucrose liquid through glutaraldehyde cross-linking process.Use glutaraldehyde as linking agent in the immobilized enzyme making method of this patent, indicate glutaraldehyde in network encyclopaedia and have harm to HUMAN HEALTH: glutaraldehyde has strong impulse effect to eyes, skin and mucous membrane, and suction can cause larynx, bronchial inflammation, chemical pneumonitis, pulmonary edema etc.; Therefore, this patent immobilized enzyme method uses and there is food safety hidden danger defect in food processing field.
Chinese patent CN101200750A, rhubarb horsetails Erwinia sp and preparing the application in Palatinose, a kind of rhubarb horsetails Erwinia sp (Erwinia rhapontici) NX-5 of this disclosure of the invention and utilize this bacterium to produce sucrose isomerase and then prepare the method for Palatinose.Process for fixation in patent is: the sodium alginate of preparation 1 ~ 3% (i.e. 1 ~ 3g/100mL) concentration, add the cell (namely adding the cell of 10 ~ 25 grams in every 100mL sodium alginate soln) of 10 ~ 25%, and add 5 ~ 10% diatomite (namely adding the diatomite of 5 ~ 10 grams in every 100mL sodium alginate soln), then be the CaCl of 1 ~ 4% by concentration
2solution (g/100mL), by fixed-type for embedded material sodium alginate, reacts in this, as enzyme source.Specifically: the cell containing sucrose isomerase after the free cell containing sucrose isomerase or immobilization is filled in retort, add the sucrose solution of 45 ~ 600g/L, or loaded in filling type column type reactor by the immobilized cell obtained according to above-mentioned process for fixation, the volume of post is not limit.This reactor can 0.5 ~ 5mL/min way flow to add or circulation Continuous Flow adds the sucrose solution of 450 ~ 600g/L, enzymatic conversion reaction is carried out under the condition of 25 ~ 35 DEG C, reaction times 10 ~ 15h, by adjustment flow velocity, control the transformation efficiency of sucrose between 90 ~ 100%, to collect effluent liquid, condensing crystal obtains Palatinose.Immobilized bacterium body method and the sucrase method for transformation production efficiency of this patent are low, are only applicable to using when making a small amount of sample.Therefore, there is the defect that difficulty is produced in Palatinose industrialization in this patented method.
Chinese patent CN101591689A, the method of producing isomaltulose by transforming sucrose by biological enzymatic method, the described immobilized cell of this invention fully mixes with sucrose solution in retort, immobilized cell is suspended in sucrose solution, and apply to comprise mechanical stirring, pressurized air stirs simultaneously, the stirring actions such as recycle pump, fixation cell karyon sucrose solution is fully contacted, accelerates conversion rate, until the Palatinose content in conversion fluid reaches more than 85%.The method applies many physical property consumingly and stirs behavior, easily causes the mechanical injuries of immobilized cell, fragmentation, makes the enzymic activity rapid loss of immobilized cell and shortens work-ing life of immobilized cell, causing the significantly rising of production cost.Therefore, there is the high defect of Palatinose industrialization production cost in this patented method.
Chinese patent CN102071235A, ultrasonic wave is utilized to promote the method for zymin Efficient Conversion Palatinose, the thalline embedded immobilization of this invention and conversion process are: the sodium alginate soln of cell underflow 10mL and 10mL massfraction 2 ~ 4% and 100 ~ 1000mg glass fiber powder (about 50 ~ 1000 μm) are mixed, be added drop-wise to the CaCl of 200mL 0.2mol/L with syringe
2in solution, leave standstill solidification at 25 ~ 30 DEG C after 2 hours, filtration washing; Granule being moved to 200mL massfraction is in the glutaraldehyde solution of 0.1 ~ 0.2%, after being cross-linked 2 hours, after filtration washing, obtains spherical immobilized enzyme in 33 ~ 37 DEG C; Immobilized enzyme to be loaded in conversion tank and to fix, remaining non-contaminated state; Be provided with ultrasonic transmitter in conversion tank, certain frequency can be launched and intensity can adjust setting as required.This patent uses the method for syringe making immobilization particle body consuming time, is not suitable for suitability for industrialized production; Meanwhile, indicate glutaraldehyde in network encyclopaedia and have harm to HUMAN HEALTH: glutaraldehyde has strong impulse effect to eyes, skin and mucous membrane, and suction can cause larynx, bronchial inflammation, chemical pneumonitis, pulmonary edema etc.Therefore, this patent immobilized enzyme method uses in food processing field that also exist cannot the defect such as industrialization and food safety hidden danger.
Chinese patent CN102286454A, method for preparing isomaltulose by polyvinyl alcohol immobilized sucrose isomerase producing bacteria, a kind of method for preparing isomaltulose by polyvinyl alcohol immobilized sucrose isomerase producing bacteria of this disclosure of the invention, utilize polyvinyl alcohol or/and diatomite ratio enzyme carrier 4 ~ 50% (W/W) aqueous solution slurries that are 2:0.8 ~ 1.21, add temperature 25 ~ 30 DEG C and produce sucrose isomerase wet thallus 5 ~ 30% (W/W), stirring, it is for subsequent use to be placed on less than-20 DEG C sclerosis, during use, immobilized enzyme bacterium is taken out under being placed in room temperature and naturally thaw, then particle is cut into, add sucrose solution isomery to transform, again with producing sucrose residual in sucrose isomerase decomposition and inversion liquid, then decolouring and ion-exchange is adopted to carry out purifying conversion fluid, through condensing crystal, obtained Palatinose product after centrifugation.This patent uses polyvinyl alcohol to prepare immobilized enzyme, and collecting polyvinyl alcohol in content at network encyclopaedia entry has hazardness to HUMAN HEALTH, sucks, takes in or is harmful to health after skin absorbs, have hormesis to eyes and skin.Therefore, this patent immobilized enzyme method uses and there is food safety hidden danger defect in food processing field.
Palatinose disclosed in above patent prepares adopted thalline process for fixation and preparation technology has the following disadvantages:
1, immobilization operating process is loaded down with trivial details, consuming time, cost is high, and method is only applicable to prepare a small amount of immobilized enzyme, cannot promote the use of in suitability for industrialized production;
2, fixation support employs some harmful chemical materialss and constitutes harm to food safety, as glutaraldehyde, polyvinyl alcohol etc.;
3, in sucrose isomerase process, adopt mechanical stirring mode consumingly, easily cause immobilized thallus broken, be unfavorable for the activity keeping immobilized thallus for a long time, thus add production cost.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the object of this invention is to provide a kind of easy and simple to handle, facility investment is few, production cost is low, and without food safety hidden danger, the preparation method that can realize the Palatinose of suitability for industrialized production.
For achieving the above object, the present invention adopts following technical proposals:
A preparation method for Palatinose, step is as follows:
(1) take sucrose solution as raw material, sucrose solution and the immobilized thallus with α-glucose based transferase are mixed in conversion tank, the add-on of immobilized thallus is 5 ~ 10% (W/W) of sucrose solution; Pass into aseptic compressed air, be transform under the condition of 25 ~ 32 DEG C and pH5 ~ 7.5 in temperature, terminate when sucrose residual quantity is below 2% in conversion fluid to transform;
(2) by the conversion fluid of step (1) through decolorization filtering and ion-exchange cleaning section, then through concentrated, decrease temperature crystalline, obtain Palatinose crystal.
In step (1), described sucrose solution is the sucrose solution of Brix40 ~ 60%;
In step (1), described in there is the immobilized thallus of α-glucose based transferase preparation method be:
1) sodium alginate soln is prepared: every 100 ㎏ water add 2 ~ 6 ㎏ sodium alginates, and stir into homogeneous colloids to sodium alginate soln, stirring velocity is 50 ~ 300r/min, churning time 10 ~ 30 minutes;
2) calcium chloride solution preparation: every 100 ㎏ water add 5 ~ 10 ㎏ calcium chloride, 20 ~ 30 ㎏ sucrose, stirring and dissolving;
3) cell underflow and sodium alginate soln 1:(1 ~ 3 by volume of the bacterium of α-glucose based transferase can be produced) mix, stir speed per hour 50 ~ 100r/min, churning time 5 ~ 15 minutes;
4) the cell underflow mixed and sodium alginate mixture are moved in granulation device, the spherical thalline to corynebacterium of 5 ~ 10mm is obtained by granulation device, thalline is moved into being fixed in calcium chloride solution, and the immobilization time is 2 ~ 4 hours, being fixed thalline;
Further, described in there is the preparation method of the immobilized thallus of α-glucose based transferase, also comprise step 5) sucrose solution of thalline concentration Brix25-35% good for immobilization is washed; Remove the impurity such as chlorion, calcium ion be attached on immobilized thallus surface, alleviate the pressure of filtration, ion-exchange process in subsequent technique.
Step 3) in, the described preparation method that can produce the cell underflow of α-glucose based transferase is:
The bacterium that can produce α-glucose based transferase is seeded in fermention medium, and expand by multistage the thalline that fermentation obtains aequum, leavening temperature is 25 DEG C ~ 30 DEG C, pH6.0 ~ 7.5, fermentor tank air flow 0.3 ~ 1.2m
3/ min, stirring velocity 100 ~ 300r/min, incubation time 12 ~ 48 hours;
Undertaken centrifugal by the thalline after fermentation, collecting cell underflow, to obtain final product; Centrifuge drum is separated smallest particles and is less than 0.5 micron, centrifugal turn of centrifugal employing 10000 ~ 15000r/min degree, and whole collecting cell underflow process temperature controls at 15 ~ 30 DEG C.
The described bacterium that can produce α-glucose based transferase is selected from protaminobacter ruber (Protaminobacter rubrum), general city Serratia (Serratia plymuthica), rhubarb horsetails Erwinia sp (Erwinia rhapontici) or klebsiella (Klebsiella sp.).
Step 4) in, described granulation device comprises cylindrical shell, and cylinder body bottom is provided with the aperture that aperture is 2 ~ 5mm, and perforated area accounts for the 90-95% of cylinder body bottom area, is provided with the cavity holding material in cylindrical shell; The piston identical with cylinder internal diameter is connected with depression bar, and is moved reciprocatingly in cylindrical shell by depression bar.
In step (1), described conversion tank is the stainless cylinder of steel with heat exchange function, comprises tank body, and described tank wall is provided with chuck layer, the bottom of tank body is provided with the net device of interception immobilized thallus, and the below of described net device is provided with aseptic compressed air inlet pipe.
Further, be provided with heat transfer tube in described tank body, described heat transfer tube is tubulation, coil pipe or coiled pipe.
The temperature regulating conversion fluid in stainless cylinder of steel is exchanged by the cold water of heat transfer tube in chuck and/or tank or the cold and hot of hot water.
Described net device refers to stainless steel card or the stainless steel mesh of full hole, and hole can be the shapes such as circular, square, trilateral, about hole diameter 2mm.
Described aseptic compressed air refers to the pressurized air after dedusting, oil water removal and microbiological free air filter process, and under 0.04Mpa condition, gas flow is 0.5 ~ 1.5m
3/ min.
Beneficial effect of the present invention:
(1) the present invention adopts alginate calcium to fix sucrose isomerase to prepare Palatinose, and immobilized enzyme can be reused, and immobilized enzyme production cost is low, and without food safety hazard.
(2) immobilized bacterium production procedure, adopts mechanical stirring mode to accelerate sodium alginate soln gelatinizing process, shortens the immobilization time, enhance productivity; Adopt granulation device to make the method for immobilized thallus particle, facility investment is few, production efficiency is high, is conducive to Palatinose Industry Promotion.
(3) Palatinose conversion process, adopts and pass into air manner bottom conversion tank, and immobilized thallus is rolled in sucrose solution, is conducive to sucrose isomerase and fully contacts with sucrose molecules, makes Sucrose conversion more than 99%; And immobilized thallus has no mechanical damage, be conducive to the enzymic activity keeping immobilized thallus for a long time, thus fermentation times prepared by minimizing sucrose isomerase, can effectively reduce Palatinose production cost.
Accompanying drawing explanation
Fig. 1 is the structural representation of granulation device;
Fig. 2 a is conversion tank structural representation;
Fig. 2 b is the sectional view of conversion tank;
Wherein, 11-aperture, 12-material, 13-cylindrical shell, 14-depression bar, 15-piston;
21-aseptic compressed air inlet pipe, 22-conversion tank chuck layer, 23-net device, 24-heat transfer tube, 25-tank body, 26-water-in, 27-water outlet.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, should be noted that following explanation is only to explain the present invention, not limiting its content.
Embodiment 1: the technological process preparing Palatinose
(1) general city Serratia (Serratia plymuthica) (ATCC15928) bacterial classification is activated to three grades of shaking flask kinds from slant strains, each add-on is 10% (v/v) of total amount, proceed to 200L seeding tank fermentation culture 18 hours again, go to 5m
3fermentor tank enlarged culturing 24 hours, seeding tank is identical with the parameter of fermentor tank: leavening temperature is 25 DEG C ~ 30 DEG C, pH6.0 ~ 7.5, stirring velocity 100 ~ 300r/min.Fermention medium forms: sucrose 4%, corn steep liquor 3%, yeast extract 0.3%, Repone K 0.2%, surplus are water, pH 6.0 ~ 7.0; Charge amount 70%, air flow are 0.2 ~ 1.2m
3/ min.
(2) with GQ series of high speed tubular-bowl centrifuge, the fermented liquid centrifugation of previous step gained is obtained cell underflow, whizzer revolution 15000 revs/min, centrifuge drum is separated smallest particles and is less than 0.5 micron, whole collecting cell underflow process temperature controls at 15 ~ 30 DEG C, collects to obtain 53Kg cell underflow.
(3) by step (2) obtain the sodium alginate soln electric mixer that cell underflow 53Kg and 110Kg massfraction are 5% and carry out being stirred to fully mixing, make thalli granule with granulation device, be added to the CaCl that 100L massfraction is 10%
2in solution, leave standstill solidification at 28 DEG C after 4 hours, filtration washing, being fixed thalline, is then filled to 4m
3in conversion tank.
Wherein, the structure of granulation device as shown in Figure 1, comprises cylindrical shell 13, and be provided with the aperture 11 that aperture is 3mm bottom cylindrical shell 13, perforated area accounts for 95% of cylinder body bottom area, is provided with the cavity holding material 12 in cylindrical shell 13; The piston 15 identical with cylinder internal diameter is hinged with depression bar 14, and is moved reciprocatingly in cylindrical shell 13 by depression bar 14.Wherein depression bar can be Manual pressure bar, also can be to make piston can do the device of reciprocating by electricity or gas.
(4) 4m is used
3the sucrose solution 2m of material-compound tank configuration concentration Brix40%
3, sterilize 10 minutes, be cooled to 30 DEG C with circulated refrigerated water for 80 DEG C.
(5) be transferred in conversion tank by the sucrose solution pump that step (4) obtains, pass into aseptic compressed air, air flow is 0.3m
3/ min, maintains conversion fluid temperature at about 25 ~ 30 DEG C by cold/hot water, control PH5.5 ~ 6.5, terminates to transform when in conversion fluid, sucrose residual quantity is below 3%; In conversion fluid, Palatinose content is 76.2%, trehalulose 14.5%, glucose 1.8%, fructose 3.1%, and other are 1.3% years old.
Wherein, conversion tank is the stainless cylinder of steel with heat exchange function, its structure as shown in Figure 2, comprise tank body 25, described tank body 25 outer wall is provided with chuck layer 22, the bottom of tank body 25 is provided with the net device 23 of interception immobilized thallus, and the below of described net device 23 is provided with aseptic compressed air inlet pipe.Be provided with heat transfer tube 24 in tank body 25, heat transfer tube comprises water-in 26 and water outlet 27.
By the temperature of conversion fluid in chuck and the cold water of heat transfer tube or the stainless cylinder of steel of cold and hot exchange adjustment of hot water.
Described net device 23 refers to the stainless steel mesh of full hole, and hole can be circular, square, prismatic, hole effective diameter about 2mm.
(6) by after previous step gained conversion fluid decolorization filtering, specific conductivity is made to reach below 0.5ms/m through ion-exchange; Then adopt efficient concentration vaporizer that material is concentrated into about Brix70%, material pump is transferred in crystallizer and carries out decrease temperature crystalline by circulated refrigerated water, 5% (v/v) crystal seed in crystallisation process, can be dropped into; Use centrifuge with cutter discharge of solid separation of material, obtain Palatinose crystal, use fluidized-bed to carry out the dry Palatinose product 423Kg obtained containing free-water 0.5%.
(7) repeating step (4) ~ (6) 20 times, gained Palatinose product average content reaches more than 98.5%, and immobilized thallus enzyme activity does not obviously reduce situation, and immobilized thallus particle is without collapsing and dissolution phenomena.
In actual process production process, need in calcium chloride solution, add appropriate calcium chloride and sucrose.For 100 ㎏ calcium chloride solutions, often shift out 100 ㎏ immobilized thallus and add 2 ~ 4 ㎏ calcium chloride and 10 ~ 20 ㎏ sucrose.
Embodiment 2: the technological process preparing Palatinose
(1) be fermented bacterium by rhubarb horsetails Erwinia sp (Erwinia rhapontici) (NCPPB 1578) bacterial classification, three grades of shaking flask kinds are activated to from slant strains, each add-on is 10% (v/v) of total amount, proceed to 200L seeding tank fermentation culture 18 hours again, go to 5m
3fermentor tank enlarged culturing 24 hours, seeding tank is identical with the parameter of fermentor tank.Fermention medium sucrose 4%, corn steep liquor 3%, yeast extract 0.5%, surplus are water, PH 6.5 ~ 7.2, and charge amount 70%, air flow are 0.5m
3/ min.
(2) 75.8Kg cell underflow is collected to obtain by the method for embodiment 1 step (2).
(3) by the method for embodiment 1 step (3), gained cell underflow and 150Kg sodium alginate soln are carried out thalline immobilization.
(4) by the method preparation sucrose solution of embodiment 1 step (4).
(5) carry out sucrose inversion by the method for embodiment 1 step (5), in conversion fluid, Palatinose content is 78.5%, trehalulose 17.3%, glucose 0.3%, fructose 1.4%, and other are 0.5% years old.
(6) by the technological process of embodiment 1 step (6), the Palatinose product 441Kg containing free-water 0.3% is obtained.
(7) repeating step (4) ~ (6) 25 times, gained Palatinose product average content reaches more than 98.7%, and immobilized thallus enzyme activity does not obviously reduce situation, and immobilized thallus particle is without collapsing and dissolution phenomena.
Claims (10)
1. a preparation method for Palatinose, is characterized in that, step is as follows:
(1) take sucrose solution as raw material, sucrose solution and the immobilized thallus with α-glucose based transferase are mixed in conversion tank, the add-on of immobilized thallus is 5 ~ 10% of sucrose solution; Pass into aseptic compressed air, be transform under the condition of 25 ~ 32 DEG C and pH5 ~ 7.5 in temperature, terminate when sucrose residual quantity is below 2% in conversion fluid to transform;
(2) by the conversion fluid of step (1) through decolorization filtering and ion-exchange cleaning section, then through concentrated, decrease temperature crystalline, obtain Palatinose crystal.
2. the preparation method of Palatinose as claimed in claim 1, it is characterized in that, in step (1), described sucrose solution is the sucrose solution of Brix40 ~ 60%.
3. the preparation method of Palatinose as claimed in claim 1, is characterized in that, in step (1), described in there is the immobilized thallus of α-glucose based transferase preparation method be:
1) sodium alginate soln is prepared: every 100 ㎏ water add 2 ~ 6 ㎏ sodium alginates, and stir into homogeneous colloids to sodium alginate soln, stirring velocity is 50 ~ 300r/min, churning time 10 ~ 30 minutes;
2) calcium chloride solution preparation: every 100 ㎏ water add 5 ~ 10 ㎏ calcium chloride, 20 ~ 30 ㎏ sucrose, stirring and dissolving;
3) can produce the cell underflow of the bacterium of α-glucose based transferase and sodium alginate soln by volume 1:1 ~ 1:3 mix, stir speed per hour 50 ~ 100r/min, churning time 5 ~ 15 minutes;
4) the cell underflow mixed and sodium alginate mixture are moved in granulation device, the spherical thalline to corynebacterium of 5 ~ 10mm is obtained by granulation device, thalline is moved into being fixed in calcium chloride solution, and the immobilization time is 2 ~ 4 hours, being fixed thalline.
4. the preparation method of Palatinose as claimed in claim 3, is characterized in that, step 3) in, the described preparation method that can produce the cell underflow of α-glucose based transferase is:
The bacterium that can produce α-glucose based transferase is seeded in fermention medium, and expand by multistage the thalline that fermentation obtains aequum, leavening temperature is 25 DEG C ~ 30 DEG C, pH6.0 ~ 7.5, fermentor tank air flow 0.3 ~ 1.2m
3/ min, stirring velocity 100 ~ 300r/min, incubation time 12 ~ 48 hours;
Undertaken centrifugal by the thalline after fermentation, collecting cell underflow, to obtain final product.
5. the preparation method of Palatinose as claimed in claim 4, it is characterized in that, when carrying out centrifugal to the thalline after fermentation, centrifuge drum is separated smallest particles and is less than 0.5 micron, centrifugal turn of centrifugal employing 10000 ~ 15000r/min degree, whole collecting cell underflow process temperature controls at 15 ~ 30 DEG C.
6. the preparation method of Palatinose as claimed in claim 3, it is characterized in that, the described bacterium that can produce α-glucose based transferase is selected from protaminobacter ruber, general city Serratia, rhubarb horsetails Erwinia sp or klebsiella.
7. the preparation method of Palatinose as claimed in claim 3, is characterized in that, step 4) in, described granulation device comprises cylindrical shell, cylinder body bottom is provided with the aperture that aperture is 2 ~ 5mm, and perforated area accounts for the 90-95% of cylinder body bottom area, is provided with the cavity holding material in cylindrical shell; The piston identical with cylinder internal diameter is connected with depression bar, and is moved reciprocatingly in cylindrical shell by depression bar.
8. the preparation method of Palatinose as claimed in claim 1, it is characterized in that, in step (1), described conversion tank comprises tank body, described tank wall is provided with chuck layer, the bottom of tank body is provided with the net device of interception immobilized thallus, and the below of described net device is provided with aseptic compressed air inlet pipe.
9. the preparation method of Palatinose as claimed in claim 8, it is characterized in that, be provided with heat transfer tube in described tank body, described heat transfer tube is tubulation, coil pipe or coiled pipe.
10. the preparation method of Palatinose as claimed in claim 1, it is characterized in that, described aseptic compressed air refers to the pressurized air after dedusting, oil water removal and microbiological free air filter process, and under 0.04Mpa condition, gas flow is 0.5 ~ 1.5m
3/ min.
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| CN105907818A (en) * | 2016-06-17 | 2016-08-31 | 青岛哈尼康生物科技有限公司 | Isomaltulose production process |
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Application publication date: 20150819 |