CN104844742A - Preparation method for polystyrene (PS) microsphere used for nucleic acid amplification in genome sequencing - Google Patents
Preparation method for polystyrene (PS) microsphere used for nucleic acid amplification in genome sequencing Download PDFInfo
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Abstract
The invention belongs to the field of genome sequencing, and specifically relates to a preparation method for a PS microsphere applied to nucleic acid amplification in genome sequencing. The polystyrene microsphere has a particle size of 25 microns. The preparation method comprises the following concrete steps: (1) adding a dispersing agent into a reaction medium, then introducing nitrogen, and carrying out stirring to obtain a homogeneous system; and (2) adding styrene monomers and an initiator into a reaction system, heating the reaction system, then carrying out constant temperature reaction under a stirring condition, and carrying out cooling to room temperature, washing and drying. The monodisperse PS microsphere successfully prepared by using a dispersion-copolymerization method provided by the invention has a particle size of 25 microns and a low dispersion coefficient.
Description
Technical field
The invention belongs to gene order-checking field, be specifically related to a kind of preparation method being applied to the polystyrene microsphere of gene order-checking nucleic acid amplification.
Background technology
As one of most important invention of life science in last century, DNA sequencing technologies profoundly changes people to the understanding of life quintessence and control ability, has greatly promoted the development of global life science research.Were it not for sequencing technologies, gene order just cannot be determined, the investigative techniques such as enzyme is cut, clone, reverse transcription, cDNA, PCR, SNP, RNAi are not just known where to begin at all yet, and life science does not have the flourish of today.The life science GenBank that All the world knows, and the Human Genome Project that the global cooperation lasting 13 years costs 300,000,000 dollars completes, be based upon on the basis of order-checking invariably.
Since Sanger in 1977 has invented the technology of the two deoxidations termination principles mensuration nucleotide sequences that make use of DNA polysaccharase, sequencing technologies has been updated, and novel method is come out one after another, and order-checking scale also constantly expands.The simple operation of sequencing technologies, with low costization and high-throughput change into the developing direction into sequencing technologies.SOLiD, 454 and the high throughput sequencing technologies of new generation that is representative such as Solexa, divide the world of sequencing equally with the attitude of three state's tripartite confrontations.Three kinds of technology are each has something to recommend him, and the speed that development upgrades is with rapid changepl. never-ending changes and improvements.But presently, the process prepared of its sequencing library is all still comparatively complicated.
The preparation of sequencing library as first in whole sequencing procedure and and important step, have conclusive effect to the quality of sequencing result.The preparation of sequencing library generally comprises following step.The first step how to be extracted in high quality from sample to be detected by nucleic acid.For different samples, the experiment flow selected by people and mode will have larger difference; Second step how the nucleic acid of long segment is carried out the process of small segment flower, mainly for the RNA equal samples of genomic dna and long segment, because sequenator is for reading long restriction at present, can only check order to the random small segment of preparation, spliced by bioinformatics method again, draw the sequence information of full-length genome.Current nucleic acid small segmentization uses physical action to carry out substantially, and ultrasonic wave becomes due to the various advantage of himself universal way smashing DNA, by the small pieces segment DNA regulating ultrasonic power and ultrasonic time to obtain different lengths.3rd step how the two ends of small segment nucleic acid is connected the universal linker sequence needed for above checking order; 4th step how the nucleotide sequence being connected with joint sequence is carried out the amplification of unit molecule multiple copied, includes emulsion-based PCR, bridge-type PCR and rolling circle amplification etc., guarantees the raw information of real reflected sample while expanding order-checking strength of signal.And emulsion-based PCR generally adopts microballoon to catch a template DNA to a bead, utilize emulsion-based PCR to increase single template, same template is increased on a microballoon up to a million templates clones.
In general, the microballoon adopted in emulsion-based PCR is polystyrene (PS) microballoon, and carries out Streptavidin modification at microsphere surface, thus makes it can catch biotin labeled DNA molecular.At present, the method for synthesizing single dispersing PS microballoon has microemulsion polymerization method, emulsion polymerization, dispersion copolymerization method, suspension polymerization and seeded polymerization etc.But existing synthetic method be all linking agent and monomer mixing after be polymerized again, the polymer microballoon polymkeric substance of this way synthesis is overall crosslinked, often sphericity is poor, and size tunable is low, the requirement thus usually needing sorting just can reach size distribution deviation to be less than 5%.In order to synthesize the microballoon obtaining being cross-linked, but do not have influence on sphericity and the monodispersity of microballoon, although Japanese Patent JP58-106554 and JP63-191818 proposes the method for seeding polymerization, namely first obtain seed by letex polymerization, then increase, expand particle.The shortcoming of present method be microballoon in process of growth, produce secondary particle, need screening removing, cause product yield to reduce, complicated operation, less economical.Therefore, it is very difficult that the micron size that obtain uniform particle diameter has crosslinked polymer microballoon particle.
The polymer microballoon difficulty that synthesis has monodispersity is very large, its severe reaction conditions, complicated process of preparation, and reaction controlling requires abnormal strict.The prior synthesizing method of polymer microballoon has emulsion polymerization and suspension polymerization, emulsion polymerization can only prepare the microballoon being less than 500nm, although microballoon particle diameter prepared by suspension polymerization is at 100-1000 μm, but polymolecularity, even if be also difficult to obtain monodispersed microballoon through repeatedly sieving, the dispersion polymerization that nineteen seventies grows up and seeding polymerization can prepare particle diameter 1-100 μm, and there is the polymer microballoon of monodispersity, it is the better method preparing monodisperse polymer microballoon at present, but diffuse-aggregate reaction medium need with organic solvent as ethanol etc., also need to add stablizer as polyvinylpyrrolidone etc., agents useful for same makes troubles to the purity of polymerisate and aftertreatment.
Summary of the invention
An object of the present invention is to provide a kind of preparation method of polystyrene microsphere, its microballoon prepared can be used for the nucleic acid amplification in the preparation of gene order-checking library, and described microspherulite diameter is 25 microns, and concrete steps are as follows:
(1) dispersion agent is added in reaction medium, pass into nitrogen; Be stirred to homogeneous system;
(2) styrene monomer and initiator are added in reaction system; Reaction system is heated up; Then isothermal reaction is carried out under agitation; Be cooled to room temperature, washing, drying.
In one embodiment of the invention, described reaction medium is selected from the one or more kinds of arbitrary combination of water, ethanol, ethylene glycol monomethyl ether and methyl alcohol.In preferred embodiments, described reaction medium is water and ethylene glycol monomethyl ether, and wherein, the weight percent of water and ethylene glycol monomethyl ether is 10 ~ 90% and 10 ~ 90%; Also can be 20 ~ 40% and 60 ~ 80%, be preferably 23% and 77%.
Described dispersion agent is the one or more kinds of arbitrary combination in sodium phosphate, polyvinylpyrrolidone, polyoxyethylene glycol, polyacrylic acid, polyvinyl alcohol and hydroxypropylcellulose.In preferred embodiments, described dispersion agent is made up of polyvinyl alcohol, hydroxypropylcellulose and polyoxyethylene glycol, and the weight percent of polyvinyl alcohol, hydroxypropylcellulose and polyoxyethylene glycol is 10 ~ 80%, 10 ~ 80% and 10 ~ 80%; Also can be 20 ~ 40%, 30 ~ 50% and 20 ~ 50%, be preferably 25%, 35% and 40%.
Initiator is selected from one or both the arbitrary proportion combination of Diisopropyl azodicarboxylate (AIBN) or benzoyl peroxide (BPO).In preferred embodiments, described initiator is Diisopropyl azodicarboxylate.
In another embodiment of the invention, in final reaction system, the weight percent of styrene monomer, dispersion agent, initiator and reaction medium is followed successively by the styrene monomer of 5 ~ 40%, the dispersion agent of 0.1 ~ 1%, the initiator of 0.025 ~ 0.125%, reaction medium surplus; Be preferably the styrene monomer of 32%, the dispersion agent of 0.3%, the initiator of 0.05%, reaction medium surplus.
The present invention is further in embodiment, described step (2) reaction system is warming up to 10-90 DEG C, be preferably 75 DEG C; Stirring velocity controls at 80 ~ 120 revs/min, is preferably 100 revs/min.
Preparation of Monodispersed Polystyrene Microspheres by Dispersion Polymerization affects by 4 conditions such as monomer, initiator, dispersion agent and reaction mediums, and wherein monomer and dispersion agent are two principal elements affecting size distribution.Under certain reaction conditions, along with the increase of monomer consumption and concentration, the particle diameter of polystyrene microsphere increases; Along with the increase of stabilizing agent dosage, the particle diameter of polystyrene microsphere reduces.
In gene order-checking, the particle diameter for the PS microballoon of DNA library amplifying nucleic acid amplification is generally 25 microns, and requires that monodispersity is good.But in PS method for preparing microsphere, PS microballoon monodispersity prepared by dispersion copolymerization method is good, and the particle diameter of microballoon is less, although the PS microspherulite diameter of seeded polymerization and suspension polymerization preparation is comparatively large, but the monodispersity of microballoon is poor, and dispersion coefficient is high.Therefore in the art, preparing particle diameter is that the Monodisperse Polystyrene Microspheres of 20 to 50 microns also exists the problems referred to above always.The present invention is groped by lot of experiments and unexpected discovery, and under the specific reaction conditions of one, adopt dispersion copolymerization method successfully to prepare Monodisperse Polystyrene Microspheres, particle diameter is 25 microns and dispersion coefficient is low.
Embodiment
Further will illustrate the present invention below.It is pointed out that following explanation is only illustrating the technical scheme that application claims is protected, any restriction not to these technical schemes.The content that protection scope of the present invention is recorded with appended claims is as the criterion.
the preparation of embodiment 1 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.1g, the hydroxypropylcellulose of 1.54g and 1.76g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 2 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.0g, the hydroxypropylcellulose of 1.59g and 1.81g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.81g.Be warming up to 75 DEG C, isothermal reaction 12 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 3 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the hydroxypropylcellulose of 2.64g and 1.76g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 4 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.2g, the hydroxypropylcellulose of 1.74g and 1.86g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 5 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.2g, the hydroxypropylcellulose of 1.74g and 1.86g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.6g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 6 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.2g, the hydroxypropylcellulose of 1.74g and 1.86g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.85g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 7 polystyrene microsphere
The polyoxyethylene glycol of the water of 200g, the ethylene glycol monomethyl ether of 800g, the polyvinyl alcohol of 1.1g, the hydroxypropylcellulose of 1.54g and 1.76g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 8 polystyrene microsphere
The polyoxyethylene glycol of the water of 260g, the ethylene glycol monomethyl ether of 740g, the polyvinyl alcohol of 1.1g, the hydroxypropylcellulose of 1.54g and 1.76g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 473g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 9 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.1g, the hydroxypropylcellulose of 1.54g and 1.76g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 450g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, dry, obtains single dispersing PS microballoon.
the preparation of embodiment 10 polystyrene microsphere
The polyoxyethylene glycol of the water of 230g, the ethylene glycol monomethyl ether of 770g, the polyvinyl alcohol of 1.1g, the hydroxypropylcellulose of 1.54g and 1.76g is added in reaction vessel; pass into nitrogen protection; and at room temperature stir 20 minutes to homogeneous system, then add styrene monomer 500g and Diisopropyl azodicarboxylate 0.73g.Be warming up to 75 DEG C, isothermal reaction 8 hours.Reaction terminates, and be cooled to room temperature, product is centrifugal, and uses ethanol repetitive scrubbing, and dry, obtain single dispersing PS microballoon, particle diameter is 25.0 μm, dispersion coefficient 1.7%.
the sign of polystyrene microsphere prepared by embodiment 1-10
JSM-6700F scanning electronic microscope is adopted to measure the particle diameter of obtained PS microballoon and size distribution.With 100 microballoons for benchmark, the median size of measuring and calculating microballoon and size distribution, formula is as follows:
In formula: δ is standard variance; d
ifor the diameter of single particle; D is the mean diameter of particle; N is number of particles, f
sfor dispersion coefficient, concrete outcome is as follows:
Content of the present invention merely illustrates some claimed specific embodiments; one of them or more described technical characteristic can be combined with arbitrary one or more technical scheme in technical scheme; these technical schemes obtained through combination also in the application's protection domain, just as these technical schemes obtained through combination in the disclosure of invention concrete record.
Claims (10)
1. a preparation method for polystyrene microsphere, its microballoon prepared can be used for the nucleic acid amplification in the preparation of gene order-checking library, and described microspherulite diameter is 25 microns, and concrete steps are as follows:
(1) dispersion agent is added in reaction medium, pass into nitrogen; Be stirred to homogeneous system;
(2) styrene monomer and initiator are added in reaction system; Reaction system is heated up; Then isothermal reaction is carried out under agitation; Be cooled to room temperature, washing, drying.
2. reaction medium described in is selected from the one or more kinds of arbitrary combination of water, ethanol, ethylene glycol monomethyl ether and methyl alcohol.
3. the dispersion agent described in is the one or more kinds of arbitrary combination in sodium phosphate, polyvinylpyrrolidone, polyoxyethylene glycol, polyacrylic acid, polyvinyl alcohol and hydroxypropylcellulose.
4. initiator is selected from one or both the arbitrary proportion combination of Diisopropyl azodicarboxylate (AIBN) or benzoyl peroxide (BPO).
5. the preparation method of polystyrene microsphere according to claim 1, it is characterized in that, described reaction medium is water and ethylene glycol monomethyl ether, and wherein, the weight percent of water and ethylene glycol monomethyl ether is 10 ~ 90% and 10 ~ 90%; Also can be 20 ~ 40% and 60 ~ 80%, be preferably 23% and 77%.
6. the preparation method of polystyrene microsphere according to claim 1, it is characterized in that, described dispersion agent is made up of polyvinyl alcohol, hydroxypropylcellulose and polyoxyethylene glycol, and the weight percent of polyvinyl alcohol, hydroxypropylcellulose and polyoxyethylene glycol is 10 ~ 80%, 10 ~ 80% and 10 ~ 80%; Also can be 20 ~ 40%, 30 ~ 50% and 20 ~ 50%, be preferably 25%, 35% and 40%.
7. the preparation method of polystyrene microsphere according to claim 1, is characterized in that, initiator is selected from one or both the arbitrary proportion combination of Diisopropyl azodicarboxylate (AIBN) or benzoyl peroxide (BPO).
8. in preferred embodiments, described initiator is Diisopropyl azodicarboxylate.
9. the preparation method of polystyrene microsphere according to claim 1, it is characterized in that, in final reaction system, the weight percent of styrene monomer, dispersion agent, initiator and reaction medium is followed successively by the styrene monomer of 5 ~ 40%, the dispersion agent of 0.1 ~ 1%, the initiator of 0.025 ~ 0.125%, reaction medium surplus; Be preferably the styrene monomer of 32%, the dispersion agent of 0.3%, the initiator of 0.05%, reaction medium surplus.
10. the preparation method of polystyrene microsphere according to claim 1, is characterized in that, in final reaction system, described step (2) reaction system is warming up to 10-90 DEG C, be preferably 75 DEG C; Stirring velocity controls at 80 ~ 120 revs/min, is preferably 100 revs/min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108250336A (en) * | 2018-01-23 | 2018-07-06 | 湖北新纵科病毒疾病工程技术有限公司 | A kind of preparation method of polystyrene microsphere |
| CN108264601A (en) * | 2018-01-23 | 2018-07-10 | 湖北新纵科病毒疾病工程技术有限公司 | A kind of preparation method and applications of carboxylic polystyrene microsphere |
| CN108929663A (en) * | 2018-06-22 | 2018-12-04 | 陕西科技大学 | Resin microsphere blocking agent and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108250336A (en) * | 2018-01-23 | 2018-07-06 | 湖北新纵科病毒疾病工程技术有限公司 | A kind of preparation method of polystyrene microsphere |
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| CN108929663A (en) * | 2018-06-22 | 2018-12-04 | 陕西科技大学 | Resin microsphere blocking agent and preparation method thereof |
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