CN104840489A - Application of andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers - Google Patents
Application of andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers Download PDFInfo
- Publication number
- CN104840489A CN104840489A CN201510280060.8A CN201510280060A CN104840489A CN 104840489 A CN104840489 A CN 104840489A CN 201510280060 A CN201510280060 A CN 201510280060A CN 104840489 A CN104840489 A CN 104840489A
- Authority
- CN
- China
- Prior art keywords
- andrias davidianus
- davidianus blanchard
- extract
- skin mucus
- blanchard skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an application of an andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers. The application is characterized in that a vacuum drying extract obtained after andrias davidianus Blanchard skin mucus is subjected to alkali liquor extraction, deproteinization, ion exchange and gel chromatographic purification has polysaccharide content of 33-35% and protein content of 67-65% by mass; the polysaccharide contains the following six monosaccharides: MaN, GlcUA, GalUA, GlcN, Glc and Gal; the protein molecular weight is 30KD. Through lung cancer cell A549 inhibition tests carried out on the andrias davidianus Blanchard skin mucus extract by adopting an MTT method, according to the mass fraction, the extract with concentration of 40mu g/ml has the inhibition ratio of 92.3% after being used for treating the lung cancer cell A549 for 48 hours.
Description
Technical field
The invention belongs to biomedicine field, Andrias davidianus Blanchard skin mucus extract is preparing the application in anti-lung-cancer medicament.
Background technology
Announce according to World Health Organization (WHO), by air pollution, the impact of the factor such as smokings, lung cancer morbidity rate and mortality rate in countries in the world all in the trend of obvious rising.At present, control based on surgery the treatment of pulmonary carcinoma, medical treatment is secondary, the combination of Chinese and Western medicine, and chemicotherapy controls, but many medicines and chemicotherapy side effect large.Being formed from animal evolutionary process in special different active component and excavate anti-pulmonary carcinoma new drug, is the new way of research and development anti-lung-cancer medicament.Andrias davidianus Blanchard (Andrias davidianus Blanchard) belongs to large-scale Amphibia, Caudata, Cryptobranchidae, there is the title of " living fossil ", for defending the infringement of pathogenic microorganism and natural enemy in natural evolution process, Andrias davidianus Blanchard skin mucus cell release mucus, covers and skin defines one natural protective barrier.Containing abundant, there is special bioactive molecule in Andrias davidianus Blanchard skin mucus thing, have that broad-spectrum is antibacterial, antitumor action.
Summary of the invention
Order of the present invention is to provide one to have anti-lung cancer activity composition, derives from Andrias davidianus Blanchard skin extract slimy, and apply in the anti-cancer therapy drug of preparation.
Realize the technical scheme of above-mentioned purpose: Andrias davidianus Blanchard more than health 3 age of artificial cultivation, after sterile water wash, mechanical stimulus scraping mucus, by mass fraction, add in mucus with 0.4molNaoH aqueous solution in 20: 1 ratios, under 45 DEG C of conditions, ultrasonic oscillation extraction 2h, filtrate simmer down to original volume is 1/5th, edible ethanol is added to concentration of alcohol 75% precipitate with ethanol in concentrated solution, 10-12h is left standstill 0-4 DEG C of condition, centrifugally remove supernatant, with SAVEG method deproteinization three to four times, with absolute ethanol washing 3 times, vacuum lyophilization Andrias davidianus Blanchard skin mucus extract.Mucus extract through DEAE-52 ion-exchange chromatography, then through G-100 sephadex chromatography purification, is determined wavelength with 280nm, collects out peak sample and concentrates, vacuum lyophilization.Mucus extraction purification thing, by SDS-polyacrylamide gel electrophoresis, has obvious protein band at 28KD molecular weight.Detect through efficient liquid phase and amino-acid analyzer, extraction purification thing contains 6 kinds of monosaccharide MaN, GlcUA, GalUA, GlcN, Glc, Gal, its molecule mole ratio is 63: 152: 40: 57: 37: 77, total sugar content is 33-35.0%, protein content is 67-65.0%, and Andrias davidianus Blanchard skin mucus extract is glycoprotein.Andrias davidianus Blanchard mucus extraction purification thing PBS buffer solution becomes 1, 10, 20, 40ug/mL, positive control is made with 1ug/ml paclitaxel, add in the logarithmic (log) phase A549 lung carcinoma cell of 5%CO2 cultivation, cultivate 48h, add 20ulMTT, continue to cultivate 4h, discard culture fluid, add the sub-wind of 150ul dimethyl to dissolve, 37 DEG C of low speed concussion 10min, with ultraviolet spectrophotometer, its absorbance is detected at 570nm place, logarithmic (log) phase A549 lung carcinoma cell suppression ratio is respectively 86.30%, 91.91%, the suppression ratio 59.6% of 92.32% positive control, Andrias davidianus Blanchard mucus extract to the suppression ratio of A549 lung carcinoma cell apparently higher than positive controls, and extract concentrations is lower than positive drug.After Andrias davidianus Blanchard mucus extract-treated A549 lung carcinoma cell 48h, by observation by light microscope form, outer form shrinkage, change garden, to come off.
The present invention is further illustrated with the following Examples, the extracting method of Andrias davidianus Blanchard skin mucus extract of the present invention and the test of suppression lung carcinoma cell.
1) by Andrias davidianus Blanchard more than health 3 age of artificial cultivation, after sterile water wash, white mucus scraping mucus is produced with mechanical stimulus Andrias davidianus Blanchard, add in mucus with 0.4molNaoH aqueous solution in 20: 1 ratios, under 45 DEG C of conditions, ultrasonic oscillation extraction 2h, filtrate simmer down to original volume is 1/5th, edible ethanol is added to concentration of alcohol 75% precipitate with ethanol in concentrated solution, leave standstill 10-12h 0-4 DEG C of condition, centrifugally remove supernatant, with SAVEG method deproteinization three to four times, with absolute ethanol washing 3 times, vacuum freezing is in dry Andrias davidianus Blanchard skin mucus extract.
2) again through DEAE-52 ion-exchange chromatography, using 0.2mol/NacL eluant solution, is determined wavelength with 280nm, collects out peak sample, is greater than the desalination of 7000D bag filter, concentrated vacuum lyophilization.
3) through SePhadexG-100 gel chromatography, being mobile phase with distilled water, is determined wavelength with 280nm, and collect out peak sample and concentrate, vacuum lyophilization becomes powder.
4) Andrias davidianus Blanchard skin mucus extract, in mass fraction, total sugar content is 33-35%, and protein content is 67-65%, and containing MaN, GlcUA, GalUA, GlcN, Glc, Gal6 kind monosaccharide in extract, molecular weight of albumen is 28.0KD.
5) mtt assay is adopted to have studied Andrias davidianus Blanchard skin mucus extract to the inhibitory action of lung cell A549, respectively with 1,10,20,40ug/mL to Andrias davidianus Blanchard skin mucus extract to lung cell A549 process 48h, 86.3% is respectively to lung carcinoma cell suppression ratio, 91.91%92.3%, show Andrias davidianus Blanchard skin mucus extract and have extremely strong inhibitory action to lung carcinoma cell.
6) by optical microscope to after extract-treated A549 lung carcinoma cell 48h, observe form, result lung carcinoma cell smaller volume, cell colony quantity is sparse, attached cell quantity reduces, and occurs shrinkage, becomes circle, show that Andrias davidianus Blanchard skin mucus extract has good anti-lung carcinoma cell effect.
Claims (3)
1. Andrias davidianus Blanchard skin mucus extract is preparing the application in anti-lung-cancer medicament.It is characterized in that Andrias davidianus Blanchard skin mucus is extracted by alkali liquor, deproteinization, vacuum drying extract after ion exchange and gel chromatography, in mass fraction, polyoses content is 33-35%, and protein content is 67-65%, containing MaN, GlcUA, GalUA, GlcN, Glc, Gal6 kind monosaccharide in polysaccharide, molecular weight of albumen is 30KD, and Andrias davidianus Blanchard skin mucus extract has inhibitory action to lung cell A549 test.
2. preparing the application in anti-lung-cancer medicament according to Andrias davidianus Blanchard skin mucus extract according to claim 1, it is characterized in that: Andrias davidianus Blanchard skin mucus extract is dissolved in PBS buffer, in mass fraction, 40ug/ml extract concentrations, after lung cell A549 process 48h, its suppression ratio reaches 92.3%.
3. preparing the application in anti-lung-cancer medicament according to Andrias davidianus Blanchard skin mucus extract according to claim 1, it is characterized in that: Andrias davidianus Blanchard skin mucus extract is making injection, capsule anti-lung-cancer medicament.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510280060.8A CN104840489A (en) | 2015-05-23 | 2015-05-23 | Application of andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510280060.8A CN104840489A (en) | 2015-05-23 | 2015-05-23 | Application of andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104840489A true CN104840489A (en) | 2015-08-19 |
Family
ID=53840738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510280060.8A Pending CN104840489A (en) | 2015-05-23 | 2015-05-23 | Application of andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104840489A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769926A (en) * | 2016-02-28 | 2016-07-20 | 汉中领维生物科技发展有限公司 | Skin mucus extracts of andrias davidianus Blanchard for preparing anti-breast cancer drugs and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103621869A (en) * | 2013-11-15 | 2014-03-12 | 贵阳学院 | Giant salamander oligosaccharide peptide health-care product |
-
2015
- 2015-05-23 CN CN201510280060.8A patent/CN104840489A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103621869A (en) * | 2013-11-15 | 2014-03-12 | 贵阳学院 | Giant salamander oligosaccharide peptide health-care product |
Non-Patent Citations (4)
| Title |
|---|
| WENTAO GUO ET AL.: "major biological activities of the skin secretion of the chinese giant salamander,andrias davidianus", 《BIOLOGICAL ACTIVITIES OF ANDRIAS DAVIDIANUS SKIN SECRETION》 * |
| 徐伟良等: "大鲵皮肤黏液糖蛋白的提取纯化及抗肺癌活性研究", 《中国生化药物杂志》 * |
| 李莉等: "中国养殖大鲵的食用、药用价值及其开发利用研究进展", 《食品工业科技》 * |
| 韩雅莉等: "土鳖虫糖蛋白的提取及抗肿瘤活性初步研究", 《汕头大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769926A (en) * | 2016-02-28 | 2016-07-20 | 汉中领维生物科技发展有限公司 | Skin mucus extracts of andrias davidianus Blanchard for preparing anti-breast cancer drugs and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen et al. | Catalytic synthesis and antitumor activities of sulfated polysaccharide from Gynostemma pentaphyllum Makino | |
| CN101560267B (en) | Preparation method of polysaccharide selenite | |
| CN104672339B (en) | Cordyceps cicadae rhzomorph as well as preparation method and application thereof | |
| CN105707072A (en) | Spirulina polysaccharide and application thereof | |
| CN105769926A (en) | Skin mucus extracts of andrias davidianus Blanchard for preparing anti-breast cancer drugs and application thereof | |
| Naqash et al. | Anticoagulant, antiherpetic and antibacterial activities of sulphated polysaccharide from Indian medicinal plant Tridax procumbens L.(Asteraceae) | |
| CN103735633B (en) | A kind of preparation method of instant gardenia powder | |
| CN105037577B (en) | Procoagulant blackberry seed polysaccharide, and extraction separation method and application thereof | |
| CN104042719B (en) | A kind of antibacterial modified chitosan spray and preparation method thereof | |
| CN106188328A (en) | A kind of preparation method of carboxymethylated polysaccharides | |
| CN108047343A (en) | The preparation method and applications of Siberian fritillary bulb total starches | |
| Mzoughi et al. | Pectic polysaccharides from edible halophytes: Insight on extraction processes, structural characterizations and immunomodulatory potentials | |
| CN103588891A (en) | Preparation method and application of agaricus bisporus polysaccharide with immunocompetence | |
| CN110790848A (en) | Preparation method and application of total polysaccharides of sea buckthorn | |
| CN107602521A (en) | A kind of method and obtained OPC and purposes of pitaya peel extraction OPC | |
| CN105037578A (en) | Anticoagulant blackberry seed polysaccharides, and extraction separation method and application thereof | |
| CN104840489A (en) | Application of andrias davidianus Blanchard skin mucus extract to preparation of medicines against lung cancers | |
| CN107184875A (en) | A kind of antibacterial Chinese medicine for veterinary use preparation | |
| CN110016084A (en) | A kind of mulberry fruit proteoglycan, preparation method and the usage | |
| CN106011091A (en) | Method for extracting and purifying SOD freeze-dried powder from meat of hyriopsis cumingii | |
| CN103536622A (en) | Application of sargassum fusiforme sulphated polysaccharide in preparation of anti-aging drugs and healthcare products | |
| CN105669874A (en) | Peach gum polysaccharide degradation product PGP-2 as well as preparation method and application thereof | |
| CN103788231B (en) | A kind of method of preparing high-purity sulfuric acid Chondroitin A from rabbit ear cartilage | |
| CN104004109A (en) | Ocean sulfated glycosaminoglycan SE-3 and preparation method thereof | |
| CN109528783A (en) | The hydrolysis of chestnut spiny involucre low molecular weight polyphenol extract and purification process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150819 |
|
| WD01 | Invention patent application deemed withdrawn after publication |